Rw7G000960

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011991
Physical Location & Seq
Forward (+)
814175 .. 815199
1025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000960.1

Sequence Viewer

Length: 546 bp
ATGCAGTTGGAGAACATGGTCTCCTTGGTTTCCCGGACCGGAAGGCATTTGCAGCGCTACGATAAAGGCTGCCGCCAAGTTGTAGGATGCATACCCTACAGATATAGGCAAACAAAACAGGAATCTTTATCTACGGAGGAAATAGAAGTTCTTGTGATTAGCTCACAAAATGGTAAAGGAATGTTGTTCCCAAAGGGAGGTTGGGAACTTGATGAGTCCATTGAAGAAGCGGCTTACAGAGAAACGCTAGAGGAGGCTGGTGTGACTGGCCATCTTGAGCCTAAATTGGGCAAATGGCGTTACAAGAGCAAAAGCCAGGGCACTTTTCATGAGGGGTACATGTTCCCTCTGCTTGTCAACAAGCAGTTCGAGTCTTGGCCTGAGAAGACTGTCAGGAAACGAGAATGGGTGACTCTTGCTGAAGCTAAAGAAGCATGCCAAAATACCAAAAGAAAGAAGAACAAGATGAGCCTGTTTGTACATAACAGAGTGAGAGAGCCATACTTCGGTTTTTGTTCAATTATTTGGACGACCAAACGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

181

Amino Acids

21.25

Weight (kDa)

9.43

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 49 - 147 3.4e-13 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011991)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18300 AT1G73540
fragaria_vesca FvH4_5g17620 FvH4_5g17620
malus_domestica MD06G1232000.v1.1 MD14G1238600.v1.1
prunus_persica Prupe.5G235900_v2.0.a1
pyrus_communis pycom06g20590 pycom14g19970
rosa_chinensis RchiOBHm_Chr7g0178041
rosa_laevigata RLG00000005479
rosa_multiflora Rmu_sc0000425.1_g000002
rosa_roxburghii Rroxscaffold_3G00275250
rosa_rugosa Rorug06G0412500 Rorug06G0412500
rosa_samantha Rh7AG012100 Rh7CG012100 Rh7DG012400
rosa_wichuraiana Rw7G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 73, 230
AcoI YGGCCR 1 cut(s) 268
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 2 cut(s) 338, 480
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 3 cut(s) 197, 287, 506
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 2 cut(s) 224, 519
AjnI CCWGG 1 cut(s) 315
AloI GAACNNNNNNTCC 1 cut(s) 37
AluBI AGCT 2 cut(s) 162, 425
AluI AGCT 2 cut(s) 162, 425
Alw26I GTCTC 1 cut(s) 25
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 2 cut(s) 268, 377
ApeKI GCWGC 2 cut(s) 52, 69
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 36
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 421
AvaII GGWCC 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 323
BalI TGGCCA 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 392
BbvI GCAGC 2 cut(s) 56, 64
BccI CCATC 1 cut(s) 279
BciT130I CCWGG 1 cut(s) 317
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 97
BfoI RGCGCY 1 cut(s) 58
BisI GCNGC 4 cut(s) 53, 70, 73, 231
BlsI GCNGC 4 cut(s) 54, 71, 74, 232
Bme1390I CCNGG 2 cut(s) 34, 317
Bme18I GGWCC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 36
BmrFI CCNGG 2 cut(s) 34, 317
BmsI GCATC 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 34
BsaI GGTCTC 1 cut(s) 25
BsaJI CCNNGG 2 cut(s) 24, 316
BsaWI WCCGGW 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 287, 506
Bse1I ACTGG 1 cut(s) 271
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 2 cut(s) 24, 316
BseGI GGATG 2 cut(s) 92, 546
BseLI CCNNNNNNNGG 3 cut(s) 197, 287, 506
BseMII CTCAG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 323
BseXI GCAGC 2 cut(s) 56, 64
BshFI GGCC 2 cut(s) 270, 379
BsiSI CCGG 2 cut(s) 34, 39
BslI CCNNNNNNNGG 3 cut(s) 197, 287, 506
BsmAI GTCTC 1 cut(s) 25
BsnI GGCC 2 cut(s) 270, 379
Bso31I GGTCTC 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 323
Bsp1407I TGTACA 1 cut(s) 478
BspACI CCGC 2 cut(s) 73, 230
BspANI GGCC 2 cut(s) 270, 379
BspCNI CTCAG 1 cut(s) 373
BspHI TCATGA 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 478
BsrI ACTGG 1 cut(s) 271
BssECI CCNNGG 2 cut(s) 24, 316
BssT1I CCWWGG 1 cut(s) 24
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 1 cut(s) 391
BstAUI TGTACA 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 1 cut(s) 381
BstF5I GGATG 2 cut(s) 92, 546
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 1 cut(s) 57
BstMAI GTCTC 1 cut(s) 25
BstMWI GCNNNNNNNGC 2 cut(s) 52, 431
BstNI CCWGG 1 cut(s) 317
BstNSI RCATGY 2 cut(s) 343, 438
BstSCI CCNGG 2 cut(s) 32, 315
BstSFI CTRYAG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 323
BstV1I GCAGC 2 cut(s) 56, 64
BstV2I GAAGAC 1 cut(s) 392
BsuRI GGCC 2 cut(s) 270, 379
BtsCI GGATG 2 cut(s) 92, 546
Cac8I GCNNGC 1 cut(s) 436
CciI TCATGA 1 cut(s) 328
CfoI GCGC 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 36
CpoI CGGWCCG 1 cut(s) 36
Csp6I GTAC 2 cut(s) 337, 479
CspI CGGWCCG 1 cut(s) 36
CviAII CATG 4 cut(s) 16, 329, 340, 435
CviQI GTAC 2 cut(s) 337, 479
DdeI CTNAG 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 36
Eco47III AGCGCT 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 441
EcoRII CCWGG 1 cut(s) 315
EcoT14I CCWWGG 1 cut(s) 24
EcoT22I ATGCAT 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 4 cut(s) 19, 332, 343, 438
FaiI YATR 8 cut(s) 17, 92, 105, 330, 341, 436, 483, 502
FatI CATG 4 cut(s) 15, 328, 339, 434
Fnu4HI GCNGC 4 cut(s) 53, 70, 73, 231
FokI GGATG 1 cut(s) 99
Fsp4HI GCNGC 4 cut(s) 53, 70, 73, 231
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 56
GluI GCNGC 4 cut(s) 53, 70, 73, 231
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 2 cut(s) 270, 379
HapII CCGG 2 cut(s) 34, 39
HhaI GCGC 1 cut(s) 57
Hin1II CATG 4 cut(s) 19, 332, 343, 438
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 4 cut(s) 122, 215, 371, 412
HpaII CCGG 2 cut(s) 34, 39
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 1 cut(s) 358
Hpy188III TCNNGA 3 cut(s) 275, 329, 394
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 3 cut(s) 4, 52, 90
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 431
HpyF3I CTNAG 1 cut(s) 381
Hsp92II CATG 4 cut(s) 19, 332, 343, 438
HspAI GCGC 1 cut(s) 55
Lsp1109I GCAGC 2 cut(s) 56, 64
LweI GCATC 1 cut(s) 77
MaeI CTAG 1 cut(s) 248
MaeIII GTNAC 3 cut(s) 262, 299, 409
MboII GAAGA 3 cut(s) 236, 397, 469
MhlI GDGCHC 1 cut(s) 323
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 2 cut(s) 284, 519
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 3 cut(s) 224, 380, 406
MnlI CCTC 6 cut(s) 130, 191, 244, 247, 325, 357
Mox20I TGGCCA 1 cut(s) 270
Mph1103I ATGCAT 1 cut(s) 92
MscI TGGCCA 1 cut(s) 270
Msp20I TGGCCA 1 cut(s) 270
MspI CCGG 2 cut(s) 34, 39
MspR9I CCNGG 2 cut(s) 34, 317
MvaI CCWGG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 52, 431
NciI CCSGG 1 cut(s) 34
NlaIII CATG 4 cut(s) 19, 332, 343, 438
NmuCI GTSAC 2 cut(s) 262, 409
NsiI ATGCAT 1 cut(s) 92
NspI RCATGY 2 cut(s) 343, 438
PaeI GCATGC 1 cut(s) 438
PagI TCATGA 1 cut(s) 328
PciI ACATGT 1 cut(s) 339
PfeI GAWTC 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 32
PkrI GCNGC 4 cut(s) 54, 71, 74, 232
PleI GAGTC 3 cut(s) 223, 379, 406
PpsI GAGTC 3 cut(s) 223, 379, 406
PscI ACATGT 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 315
PspGI CCWGG 1 cut(s) 315
PspPI GGNCC 1 cut(s) 36
RsaI GTAC 2 cut(s) 338, 480
RsaNI GTAC 2 cut(s) 337, 479
Rsr2I CGGWCCG 1 cut(s) 36
RsrII CGGWCCG 1 cut(s) 36
SatI GCNGC 4 cut(s) 53, 70, 73, 231
Sau96I GGNCC 1 cut(s) 36
SchI GAGTC 3 cut(s) 224, 380, 406
ScrFI CCNGG 2 cut(s) 34, 317
SduI GDGCHC 1 cut(s) 323
SetI ASST 3 cut(s) 164, 202, 427
SfaNI GCATC 1 cut(s) 77
SfcI CTRYAG 1 cut(s) 97
SinI GGWCC 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SphI GCATGC 1 cut(s) 438
Sse9I AATT 2 cut(s) 284, 519
SsiI CCGC 2 cut(s) 73, 230
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 32, 315
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 391
TaqI TCGA 1 cut(s) 369
TasI AATT 2 cut(s) 284, 519
TatI WGTACW 1 cut(s) 478
TauI GCSGC 2 cut(s) 75, 233
TfiI GAWTC 1 cut(s) 122
TseFI GTSAC 2 cut(s) 262, 409
TseI GCWGC 2 cut(s) 52, 69
Tsp45I GTSAC 2 cut(s) 262, 409
TspDTI ATGAA 1 cut(s) 317
TspGWI ACGGA 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 36
XceI RCATGY 2 cut(s) 343, 438
XcmI CCANNNNNNNNNTGG 1 cut(s) 198
XspI CTAG 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.