Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015480

Physical Location & Seq

Genomic Coordinates Forward (+)

729840 .. 733527

Gene Structure 3688 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1013300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1356 bp

ATGGAGATTTCTGAAAGTGAGCCCCAAATCGAATTATCTGAAAACCCTAGAGCCACCTGGGATGGTTGCTCCGTCTTGCTCGACATCAACGACGGCGACCGTCTGGTTTTCGCCCGCCTCTCCGCCGGAGCGAAAGTGAAAATTGGGAATACAAATTGCTCTCTGCAGCCCTTGATTGGCTGTCCCTTTGGCTCTTTCTTTCGGGTCGAAAACGGAGCTGAAGGCCCGTATTTGGCCCGCTACACTCCGTCCACGGAAGGTAATAATGTTCTGGAAAAAGGTGAAATTCAATCAGTAGATGAGTTTAGAGATAATCGAGCATTAGTCGATAACAATAAAGCGCAGAGCCTAACCGGTGAAGAAATTAACGCAATGCGACAACAGGGTGCAACGGGCGATGAAATAGTTGAAGCTCTCATTGCAAACAGTGCGACGTTTGAGAAGAAAACTGTTTTCTCACAGGAGAAGTATAGGCTTAGGAAGCAAAAGAAGTATGCTCCCATTGTACTTATGAGGCGCCCATACACTAGAAGTATATGTGAGGCGTACTTGAAGAAATATCCGAATAAAATTGGATTCTTGCGGATGGATACATTGTCTCTTCTGCTTTCCATGGCTAATGTTTCTGCAAATTCTGATGTCCTCGTAGTGGATATGGTTGGAGGACTTCTAACAGGTGCTGTGGCGGAGCGTTTAGGAGGTATGGGCTATGTCTGCAATACATATCTTGGTGGCTCACCTTATTCTATGGATATAGCGAGGATATTCAACTTTGGAGATGAAATTTGCAAGAGGATTGTGAGGTCTTCTCTCACTGACCTCTTATCTTTCCAAAACGGACTGCCCAAACCAGTCAGTCAGGACACGGACTCTTGTAACATGGAATTTCAGCCAAATGGCCAAATTTCTTCAGCAGTCAGTACAGAAGAAGTATCTCTTACATCTGAAAATGTGAAATCAGAACCTGTTCCCGAGTCTACCAGTTCTCCAGTAGTCAAAATATCCAAACCCGCAAAATTGGGAGAAAAAGCACCACAGGAAATCATTAGCTCGTGGAAAGAGAATGGTTTCCCTAGTTTAATAATAGCAGCACCGGAGCTGGATGCATGGAGCTTAGTTAAAGAGATCTTGCCACTTATGTCAAATTCTGCTCCTTTTGCTATTTTTCATCAGTACCTTCAGCCTCTTGCAACATGCATGCACAATTTACAGCAGGGGAAAATAGCGATTGGCATGCAAATTTCAGAACCTTGGTTACGCGAATATCAGGTGCTTCCATCAAGAACTCATCCATGCATGCAGATGAATGGATTCGGTGGGTATATTCTCAGCGGGACCAAAATTTCACCGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0030488 GO:0031515 GO:0032259 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0034708 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043527 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gcd10p

Protein Analysis

452

Amino Acids

49.56

Weight (kDa)

5.44

Isoelectric Point (pI)

47.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gcd10p	PF04189	24 - 276	4.4e-60	Gcd10p family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015480)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45730
fragaria_vesca	FvH4_2g38570
malus_domestica	MD08G1013700.v1.1 MD15G1013300.v1.1
prunus_persica	Prupe.1G366700_v2.0.a1
pyrus_communis	pycom08g01240
rosa_chinensis	RchiOBHm_Chr6g0302731
rosa_laevigata	RLG00000011143
rosa_multiflora	Rmu_sc0009897.1_g000006
rosa_roxburghii	Rroxscaffold_7G00165370
rosa_rugosa	Rorug06G0316600
rosa_samantha	Rh6AG429900 Rh6BG472600 Rh6CG442400 Rh6DG429100
rosa_wichuraiana	Rw6G037230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	516
AccI	GTMKAC	1 cut(s)	977
AccII	CGCG	1 cut(s)	1260
AciI	CCGC	7 cut(s)	115, 123, 238, 583, 686, 1011, 1332
AcoI	YGGCCR	1 cut(s)	898
AcsI	RAATTY	8 cut(s)	285, 631, 783, 884, 903, 1144, 1239, 1341
AcuI	CTGAAG	3 cut(s)	240, 894, 1163
AcyI	GRCGYC	1 cut(s)	517
AfaI	GTAC	4 cut(s)	507, 548, 922, 1175
AfiI	CCNNNNNNNGG	3 cut(s)	176, 232, 649
AgeI	ACCGGT	1 cut(s)	353
AgsI	TTSAA	4 cut(s)	290, 410, 553, 769
AjnI	CCWGG	1 cut(s)	56
AluBI	AGCT	5 cut(s)	218, 413, 1050, 1099, 1113
AluI	AGCT	5 cut(s)	218, 413, 1050, 1099, 1113
Alw26I	GTCTC	1 cut(s)	603
AlwNI	CAGNNNCTG	2 cut(s)	680, 965
Ama87I	CYCGRG	1 cut(s)	971
AoxI	GGCC	3 cut(s)	223, 234, 898
ApeKI	GCWGC	2 cut(s)	166, 1088
ApoI	RAATTY	8 cut(s)	285, 631, 783, 884, 903, 1144, 1239, 1341
AsiGI	ACCGGT	1 cut(s)	353
Asp700I	GAANNNNTTC	3 cut(s)	966, 1067, 1310
AspLEI	GCGC	2 cut(s)	343, 519
AspS9I	GGNCC	3 cut(s)	224, 235, 1335
AsuHPI	GGTGA	4 cut(s)	293, 368, 729, 1338
AvaI	CYCGRG	1 cut(s)	971
AvaII	GGWCC	1 cut(s)	1335
BalI	TGGCCA	1 cut(s)	900
BanI	GGYRCC	1 cut(s)	516
BanII	GRGCYC	1 cut(s)	24
BauI	CACGAG	1 cut(s)	1051
BbsI	GAAGAC	1 cut(s)	798
BbvI	GCAGC	2 cut(s)	178, 1100
BccI	CCATC	3 cut(s)	56, 580, 1285
BceAI	ACGGC	1 cut(s)	109
BcgI	CGANNNNNNTGC	2 cut(s)	1216, 1250
BciT130I	CCWGG	1 cut(s)	58
BciVI	GTATCC	1 cut(s)	583
BcoDI	GTCTC	1 cut(s)	603
BfaI	CTAG	3 cut(s)	48, 528, 1074
BfmI	CTRYAG	1 cut(s)	164
BfoI	RGCGCY	1 cut(s)	520
BfuI	GTATCC	1 cut(s)	583
BglII	AGATCT	1 cut(s)	1125
BisI	GCNGC	2 cut(s)	167, 1089
BlsI	GCNGC	2 cut(s)	168, 1090
Bme1390I	CCNGG	1 cut(s)	58
Bme18I	GGWCC	1 cut(s)	1335
BmeT110I	CYCGRG	1 cut(s)	971
BmgT120I	GGNCC	3 cut(s)	224, 235, 1335
BmiI	GGNNCC	2 cut(s)	518, 1336
BmrFI	CCNGG	1 cut(s)	58
BmsI	GCATC	1 cut(s)	1093
BpiI	GAAGAC	1 cut(s)	798
BplI	GAGNNNNNCTC	2 cut(s)	793, 825
BpmI	CTGGAG	1 cut(s)	972
Bpu10I	CCTNAGC	1 cut(s)	476
BsaHI	GRCGYC	1 cut(s)	517
BsaJI	CCNNGG	4 cut(s)	57, 252, 612, 1250
BsaWI	WCCGGW	2 cut(s)	353, 1093
BsaXI	ACNNNNNCTCC	4 cut(s)	120, 150, 970, 1000
Bsc4I	CCNNNNNNNGG	3 cut(s)	176, 232, 649
Bse118I	RCCGGY	1 cut(s)	353
Bse1I	ACTGG	3 cut(s)	851, 981, 989
Bse3DI	GCAATG	2 cut(s)	378, 417
BseBI	CCWGG	1 cut(s)	58
BseDI	CCNNGG	4 cut(s)	57, 252, 612, 1250
BseGI	GGATG	4 cut(s)	67, 591, 1108, 1288
BseLI	CCNNNNNNNGG	3 cut(s)	176, 232, 649
BseMI	GCAATG	2 cut(s)	378, 417
BseMII	CTCAG	1 cut(s)	1342
BseNI	ACTGG	3 cut(s)	851, 981, 989
BseXI	GCAGC	2 cut(s)	178, 1100
Bsh1236I	CGCG	1 cut(s)	1260
Bsh1285I	CGRYCG	1 cut(s)	100
BshFI	GGCC	3 cut(s)	225, 236, 900
BshNI	GGYRCC	1 cut(s)	516
BshTI	ACCGGT	1 cut(s)	353
BsiEI	CGRYCG	1 cut(s)	100
BsiHKCI	CYCGRG	1 cut(s)	971
BsiSI	CCGG	3 cut(s)	126, 354, 1094
BslFI	GGGAC	2 cut(s)	168, 1348
BslI	CCNNNNNNNGG	3 cut(s)	176, 232, 649
BsmAI	GTCTC	1 cut(s)	603
BsmFI	GGGAC	2 cut(s)	168, 1348
BsnI	GGCC	3 cut(s)	225, 236, 900
BsoBI	CYCGRG	1 cut(s)	971
Bsp1286I	GDGCHC	1 cut(s)	24
Bsp143I	GATC	1 cut(s)	1125
Bsp19I	CCATGG	1 cut(s)	612
BspACI	CCGC	7 cut(s)	115, 123, 238, 583, 686, 1011, 1332
BspANI	GGCC	3 cut(s)	225, 236, 900
BspCNI	CTCAG	1 cut(s)	1341
BspFNI	CGCG	1 cut(s)	1260
BspLI	GGNNCC	2 cut(s)	518, 1336
BspMAI	CTGCAG	1 cut(s)	168
BspT107I	GGYRCC	1 cut(s)	516
BsrDI	GCAATG	2 cut(s)	378, 417
BsrFI	RCCGGY	1 cut(s)	353
BsrI	ACTGG	3 cut(s)	851, 981, 989
BssAI	RCCGGY	1 cut(s)	353
BssECI	CCNNGG	4 cut(s)	57, 252, 612, 1250
BssMI	GATC	1 cut(s)	1125
BssNI	GRCGYC	1 cut(s)	517
BssSI	CACGAG	1 cut(s)	1051
BssT1I	CCWWGG	2 cut(s)	612, 1250
Bst2BI	CACGAG	1 cut(s)	1051
Bst2UI	CCWGG	1 cut(s)	58
Bst4CI	ACNGT	3 cut(s)	101, 428, 451
Bst6I	CTCTTC	1 cut(s)	606
BstACI	GRCGYC	1 cut(s)	517
BstAPI	GCANNNNNTGC	1 cut(s)	428
BstC8I	GCNNGC	5 cut(s)	115, 238, 1199, 1235, 1298
BstDEI	CTNAG	3 cut(s)	476, 1114, 1328
BstDSI	CCRYGG	2 cut(s)	252, 612
BstF5I	GGATG	4 cut(s)	67, 591, 1108, 1288
BstFNI	CGCG	1 cut(s)	1260
BstH2I	RGCGCY	1 cut(s)	520
BstHHI	GCGC	2 cut(s)	343, 519
BstKTI	GATC	1 cut(s)	1128
BstMAI	GTCTC	1 cut(s)	603
BstMBI	GATC	1 cut(s)	1125
BstMCI	CGRYCG	1 cut(s)	100
BstMWI	GCNNNNNNNGC	5 cut(s)	419, 428, 481, 714, 1157
BstNI	CCWGG	1 cut(s)	58
BstNSI	RCATGY	4 cut(s)	1197, 1201, 1237, 1300
BstSCI	CCNGG	1 cut(s)	56
BstSFI	CTRYAG	1 cut(s)	164
BstUI	CGCG	1 cut(s)	1260
BstV1I	GCAGC	2 cut(s)	178, 1100
BstV2I	GAAGAC	1 cut(s)	798
BstX2I	RGATCY	1 cut(s)	1125
BstYI	RGATCY	1 cut(s)	1125
BsuI	GTATCC	1 cut(s)	583
BsuRI	GGCC	3 cut(s)	225, 236, 900
BtgI	CCRYGG	2 cut(s)	252, 612
BtgZI	GCGATG	1 cut(s)	411
BtsCI	GGATG	4 cut(s)	67, 591, 1108, 1288
BtsIMutI	CAGTG	2 cut(s)	433, 813
Cac8I	GCNNGC	5 cut(s)	115, 238, 1199, 1235, 1298
CaiI	CAGNNNCTG	2 cut(s)	680, 965
CfoI	GCGC	2 cut(s)	343, 519
Cfr10I	RCCGGY	1 cut(s)	353
Cfr13I	GGNCC	3 cut(s)	224, 235, 1335
Csp6I	GTAC	4 cut(s)	506, 547, 921, 1174
CspAI	ACCGGT	1 cut(s)	353
CviAII	CATG	8 cut(s)	613, 880, 1107, 1194, 1198, 1234, 1293, 1297
CviQI	GTAC	4 cut(s)	506, 547, 921, 1174
DdeI	CTNAG	3 cut(s)	476, 1114, 1328
DinI	GGCGCC	1 cut(s)	518
DpnI	GATC	1 cut(s)	1127
DpnII	GATC	1 cut(s)	1125
EaeI	YGGCCR	1 cut(s)	898
Eam1104I	CTCTTC	1 cut(s)	606
EarI	CTCTTC	1 cut(s)	606
EciI	GGCGGA	2 cut(s)	112, 701
Eco130I	CCWWGG	2 cut(s)	612, 1250
Eco24I	GRGCYC	1 cut(s)	24
Eco47I	GGWCC	1 cut(s)	1335
Eco57I	CTGAAG	3 cut(s)	240, 894, 1163
Eco88I	CYCGRG	1 cut(s)	971
EcoRII	CCWGG	1 cut(s)	56
EcoT14I	CCWWGG	2 cut(s)	612, 1250
EcoT22I	ATGCAT	3 cut(s)	1108, 1199, 1298
EcoT38I	GRGCYC	1 cut(s)	24
EgeI	GGCGCC	1 cut(s)	518
EheI	GGCGCC	1 cut(s)	518
ErhI	CCWWGG	2 cut(s)	612, 1250
FaeI	CATG	8 cut(s)	616, 883, 1110, 1197, 1201, 1237, 1296, 1300
FalI	AAGNNNNNCTT	2 cut(s)	921, 953
FaqI	GGGAC	2 cut(s)	168, 1348
FatI	CATG	8 cut(s)	612, 879, 1106, 1193, 1197, 1233, 1292, 1296
FauI	CCCGC	4 cut(s)	122, 245, 1018, 1325
FblI	GTMKAC	1 cut(s)	977
Fnu4HI	GCNGC	2 cut(s)	167, 1089
FokI	GGATG	4 cut(s)	74, 598, 1115, 1275
FriOI	GRGCYC	1 cut(s)	24
Fsp4HI	GCNGC	2 cut(s)	167, 1089
FspBI	CTAG	3 cut(s)	48, 528, 1074
GlaI	GCGC	2 cut(s)	342, 518
GluI	GCNGC	2 cut(s)	167, 1089
GsuI	CTGGAG	1 cut(s)	972
HaeII	RGCGCY	1 cut(s)	520
HaeIII	GGCC	3 cut(s)	225, 236, 900
HapII	CCGG	3 cut(s)	126, 354, 1094
HhaI	GCGC	2 cut(s)	343, 519
Hin1I	GRCGYC	1 cut(s)	517
Hin1II	CATG	8 cut(s)	616, 883, 1110, 1197, 1201, 1237, 1296, 1300
Hin6I	GCGC	2 cut(s)	341, 517
HinP1I	GCGC	2 cut(s)	341, 517
HinfI	GANTC	4 cut(s)	576, 869, 974, 1311
HpaII	CCGG	3 cut(s)	126, 354, 1094
HphI	GGTGA	4 cut(s)	293, 368, 729, 1338
Hpy166II	GTNNAC	2 cut(s)	252, 978
Hpy188I	TCNGA	7 cut(s)	13, 40, 564, 637, 946, 961, 1246
Hpy188III	TCNNGA	4 cut(s)	272, 860, 971, 1281
Hpy8I	GTNNAC	2 cut(s)	252, 978
Hpy99I	CGWCG	2 cut(s)	95, 436
HpyAV	CCTTC	3 cut(s)	215, 251, 1187
HpyCH4III	ACNGT	3 cut(s)	101, 428, 451
HpyCH4IV	ACGT	1 cut(s)	434
HpyF10VI	GCNNNNNNNGC	5 cut(s)	419, 428, 481, 714, 1157
HpyF3I	CTNAG	3 cut(s)	476, 1114, 1328
HpySE526I	ACGT	1 cut(s)	434
Hsp92I	GRCGYC	1 cut(s)	517
Hsp92II	CATG	8 cut(s)	616, 883, 1110, 1197, 1201, 1237, 1296, 1300
HspAI	GCGC	2 cut(s)	341, 517
KasI	GGCGCC	1 cut(s)	516
Kzo9I	GATC	1 cut(s)	1125
LmnI	GCTCC	8 cut(s)	74, 128, 215, 502, 688, 1096, 1110, 1156
Lsp1109I	GCAGC	2 cut(s)	178, 1100
LweI	GCATC	1 cut(s)	1093
MaeI	CTAG	3 cut(s)	48, 528, 1074
MaeII	ACGT	1 cut(s)	434
MaeIII	GTNAC	2 cut(s)	875, 1254
MalI	GATC	1 cut(s)	1127
MboI	GATC	1 cut(s)	1125
MboII	GAAGA	7 cut(s)	371, 454, 565, 593, 798, 900, 938
MflI	RGATCY	1 cut(s)	1125
MhlI	GDGCHC	1 cut(s)	24
MlsI	TGGCCA	1 cut(s)	900
MluNI	TGGCCA	1 cut(s)	900
Mly113I	GGCGCC	1 cut(s)	517
MlyI	GAGTC	2 cut(s)	863, 983
MmeI	TCCRAC	1 cut(s)	640
Mox20I	TGGCCA	1 cut(s)	900
Mph1103I	ATGCAT	3 cut(s)	1108, 1199, 1298
MroXI	GAANNNNTTC	3 cut(s)	966, 1067, 1310
MscI	TGGCCA	1 cut(s)	900
MseI	TTAA	3 cut(s)	366, 1079, 1119
MslI	CAYNNNNRTG	1 cut(s)	1301
Msp20I	TGGCCA	1 cut(s)	900
MspA1I	CMGCKG	1 cut(s)	1332
MspI	CCGG	3 cut(s)	126, 354, 1094
MspR9I	CCNGG	1 cut(s)	58
MvaI	CCWGG	1 cut(s)	58
MvnI	CGCG	1 cut(s)	1260
MwoI	GCNNNNNNNGC	5 cut(s)	419, 428, 481, 714, 1157
NarI	GGCGCC	1 cut(s)	517
NcoI	CCATGG	1 cut(s)	612
NdeII	GATC	1 cut(s)	1125
NlaIII	CATG	8 cut(s)	616, 883, 1110, 1197, 1201, 1237, 1296, 1300
NlaIV	GGNNCC	2 cut(s)	518, 1336
NsiI	ATGCAT	3 cut(s)	1108, 1199, 1298
NspI	RCATGY	4 cut(s)	1197, 1201, 1237, 1300
PaeI	GCATGC	3 cut(s)	1201, 1237, 1300
PcsI	WCGNNNNNNNCGW	1 cut(s)	87
PdmI	GAANNNNTTC	3 cut(s)	966, 1067, 1310
PfeI	GAWTC	2 cut(s)	576, 1311
PinAI	ACCGGT	1 cut(s)	353
PkrI	GCNGC	2 cut(s)	168, 1090
PleI	GAGTC	2 cut(s)	863, 982
PluTI	GGCGCC	1 cut(s)	520
PpsI	GAGTC	2 cut(s)	863, 982
Psp6I	CCWGG	1 cut(s)	56
PspGI	CCWGG	1 cut(s)	56
PspN4I	GGNNCC	2 cut(s)	518, 1336
PspPI	GGNCC	3 cut(s)	224, 235, 1335
PsrI	GAACNNNNNNTAC	2 cut(s)	1239, 1271
PstI	CTGCAG	1 cut(s)	168
PstNI	CAGNNNCTG	2 cut(s)	680, 965
PsuI	RGATCY	1 cut(s)	1125
RsaI	GTAC	4 cut(s)	507, 548, 922, 1175
RsaNI	GTAC	4 cut(s)	506, 547, 921, 1174
RseI	CAYNNNNRTG	1 cut(s)	1301
SaqAI	TTAA	3 cut(s)	366, 1079, 1119
SatI	GCNGC	2 cut(s)	167, 1089
Sau3AI	GATC	1 cut(s)	1125
Sau96I	GGNCC	3 cut(s)	224, 235, 1335
SchI	GAGTC	2 cut(s)	863, 983
ScrFI	CCNGG	1 cut(s)	58
SduI	GDGCHC	1 cut(s)	24
SfaNI	GCATC	1 cut(s)	1093
SfcI	CTRYAG	1 cut(s)	164
SfoI	GGCGCC	1 cut(s)	518
SinI	GGWCC	1 cut(s)	1335
SmiMI	CAYNNNNRTG	1 cut(s)	1301
SphI	GCATGC	3 cut(s)	1201, 1237, 1300
SsiI	CCGC	7 cut(s)	115, 123, 238, 583, 686, 1011, 1332
SspDI	GGCGCC	1 cut(s)	516
SspMI	CTAG	3 cut(s)	48, 528, 1074
StyD4I	CCNGG	1 cut(s)	56
StyI	CCWWGG	2 cut(s)	612, 1250
TaaI	ACNGT	3 cut(s)	101, 428, 451
TaiI	ACGT	1 cut(s)	437
TaqI	TCGA	5 cut(s)	30, 81, 207, 316, 327
TatI	WGTACW	2 cut(s)	505, 920
TfiI	GAWTC	2 cut(s)	576, 1311
Tru1I	TTAA	3 cut(s)	366, 1079, 1119
Tru9I	TTAA	3 cut(s)	366, 1079, 1119
TscAI	CASTG	2 cut(s)	433, 820
TseI	GCWGC	2 cut(s)	166, 1088
TspDTI	ATGAA	4 cut(s)	414, 795, 1157, 1319
TspGWI	ACGGA	6 cut(s)	61, 228, 237, 269, 852, 881
TspRI	CASTG	2 cut(s)	433, 820
VpaK11BI	GGWCC	1 cut(s)	1335
XapI	RAATTY	8 cut(s)	285, 631, 783, 884, 903, 1144, 1239, 1341
XceI	RCATGY	4 cut(s)	1197, 1201, 1237, 1300
XmiI	GTMKAC	1 cut(s)	977
XmnI	GAANNNNTTC	3 cut(s)	966, 1067, 1310
XspI	CTAG	3 cut(s)	48, 528, 1074
Zsp2I	ATGCAT	3 cut(s)	1108, 1199, 1298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.