Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0015480

Physical Location & Seq

Genomic Coordinates Reverse (-)

61631428 .. 61635271

Gene Structure 3844 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G037230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGTCTGAAGATAAGCTCCACGGCGATGCTGTGGAAAACCCTAGACTCACCTGGGACGGTTGCAGCGTCTTGCTTGACATCAACGACGGCGACCGTTTGGTTTTCTCGCGCCTCTCCGCCGGAGCAAAATTGAAAATTGGGAATAAGAATTGCTCCCTGCAGCCGTTGATTGGCTGCCCCTTCGGTTCCTTTTTTCGAGTCGAAAACGGAGCACAAGGGCCTTATTTGTCAAGATATACTCCCTCCAATGAAGGTAATAGTATTCAAGAACAAGGGGATTGCCAATCGAAAGACGAGTTAAGAGATAATCGAGCATTGGTTGATAACAATAAAGCGCAAAGCCTAACGGGGGATGCCATCAATGAGATGCGACGACAGGGCGCAAGTGGTGATGAAATAGTTGAAGCTCTCATTGCCAACAGCGCGACGTTTGAGACAAAGACACTGTTCTCACAGGAGAAATATAGGCTTAAGAAGCAAAAAAAGTATGCTCCCATTGTACTTCTCAGGCGGCCTTTCACTAGAAGTATATGTGAGGCGTACTTGAAGAAATATCCGGTTAGAATTGGATTCTTGCGGATGGATACGTTATGTCTGCTGCTTTCCATGGCTAATGTTTCTGCTAATTCCGATGTCCTTGTAGTCGACATGGTTGGAGGGCTTCTTACTGGTGCTGTGGCAGAGCGTTTAGGAGGTACAGGTTATGTCTGCAATACATATCCTGGTGGCTCACCTTATCCTATGGATATAGCGAGGATATTCAACTTTGGAGAAGAAATTTGCAAGAGGATTGTGAGGTCTCCTCTGGCTGACCTTTTCTCCGTCCAGACTGAAAAGTCAAAAGTCTCGAGTCACAATGGGGACTCTTGTAGCATGGAAATTGAATCAAATGAACAAATATCTTCAACGGCTGGCACGGAAGAAGTTTCTCTTGCGTCTGAGAATATGATATCAGATCTTATTCCTGAAACTACCTATTCTGTAGAAAGAAAACCGAGCAAATCTGCAAAACTAGGAGAAAAAGCACCACATGAAATCATCAGCTTGTGGAAAGAGAATGGCTTTACTAGTTTAATAATAGCAGCACCAGAGCTGGATACGTGGAGTCTAGTTAAAAAGATATTGCCACTTCTGTCAAATTCTGCTCCTTTTGCTATTTATCACCAGTATCTTCAGCCGCTCGCAACATGCATGCACAATCTACAAGTGGGGAAAATGGCAGTTGGCTTGCAAATTTCAGAACCTTGGTTACGTGAATATCAGGTACTTCCATCAAGAACCCATCCATGTATGCAGATGAATGGATTTGGTGGGTACATTCTCAGCGGGACCAAGATATGTACCAACAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0030488 GO:0031515 GO:0032259 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0034708 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043527 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gcd10p

Protein Analysis

450

Amino Acids

49.58

Weight (kDa)

6.36

Isoelectric Point (pI)

44.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gcd10p	PF04189	22 - 282	8.9e-58	Gcd10p family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015480)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45730
fragaria_vesca	FvH4_2g38570
malus_domestica	MD08G1013700.v1.1 MD15G1013300.v1.1
prunus_persica	Prupe.1G366700_v2.0.a1
pyrus_communis	pycom08g01240
rosa_chinensis	RchiOBHm_Chr6g0302731
rosa_laevigata	RLG00000011143
rosa_multiflora	Rmu_sc0009897.1_g000006
rosa_roxburghii	Rroxscaffold_7G00165370
rosa_rugosa	Rorug06G0316600
rosa_samantha	Rh6AG429900 Rh6BG472600 Rh6CG442400 Rh6DG429100
rosa_wichuraiana	Rw6G037230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	835
AccBSI	CCGCTC	1 cut(s)	1180
AccI	GTMKAC	1 cut(s)	645
AccII	CGCG	2 cut(s)	109, 425
AciI	CCGC	5 cut(s)	117, 511, 577, 1178, 1326
AcsI	RAATTY	3 cut(s)	777, 1138, 1233
AcuI	CTGAAG	2 cut(s)	27, 1157
AfaI	GTAC	6 cut(s)	501, 542, 697, 1266, 1316, 1342
AfiI	CCNNNNNNNGG	2 cut(s)	170, 349
AflII	CTTAAG	1 cut(s)	470
AgsI	TTSAA	7 cut(s)	133, 266, 404, 547, 763, 884, 906
AhlI	ACTAGT	1 cut(s)	1067
AjnI	CCWGG	2 cut(s)	50, 721
AluBI	AGCT	4 cut(s)	16, 407, 1044, 1093
AluI	AGCT	4 cut(s)	16, 407, 1044, 1093
Alw21I	GWGCWC	1 cut(s)	214
Alw26I	GTCTC	3 cut(s)	428, 804, 850
Ama87I	CYCGRG	1 cut(s)	847
AoxI	GGCC	2 cut(s)	218, 512
ApeKI	GCWGC	5 cut(s)	63, 160, 174, 598, 1082
ApoI	RAATTY	3 cut(s)	777, 1138, 1233
AspLEI	GCGC	4 cut(s)	111, 337, 383, 425
AspS9I	GGNCC	2 cut(s)	218, 1329
AsuHPI	GGTGA	4 cut(s)	40, 401, 723, 1154
AvaI	CYCGRG	1 cut(s)	847
AvaII	GGWCC	1 cut(s)	1329
Bbv12I	GWGCWC	1 cut(s)	214
BbvI	GCAGC	5 cut(s)	75, 161, 172, 585, 1094
BccI	CCATC	4 cut(s)	365, 574, 1279, 1290
BceAI	ACGGC	4 cut(s)	37, 103, 148, 924
BciT130I	CCWGG	2 cut(s)	52, 723
BciVI	GTATCC	2 cut(s)	577, 1090
BcoDI	GTCTC	3 cut(s)	428, 804, 850
BcuI	ACTAGT	1 cut(s)	1067
BfaI	CTAG	5 cut(s)	42, 522, 1013, 1068, 1109
BfmI	CTRYAG	2 cut(s)	158, 981
BfrI	CTTAAG	1 cut(s)	470
BfuI	GTATCC	2 cut(s)	577, 1090
BglII	AGATCT	1 cut(s)	955
BisI	GCNGC	7 cut(s)	64, 161, 175, 512, 599, 1083, 1178
BlsI	GCNGC	7 cut(s)	65, 162, 176, 513, 600, 1084, 1179
Bme1390I	CCNGG	2 cut(s)	52, 723
Bme18I	GGWCC	1 cut(s)	1329
BmeT110I	CYCGRG	1 cut(s)	847
BmgT120I	GGNCC	2 cut(s)	218, 1329
BmiI	GGNNCC	2 cut(s)	187, 1330
BmrFI	CCNGG	2 cut(s)	52, 723
BmsI	GCATC	3 cut(s)	16, 343, 357
BplI	GAGNNNNNCTC	2 cut(s)	787, 819
BsaAI	YACGTR	2 cut(s)	1101, 1253
BsaI	GGTCTC	1 cut(s)	804
BsaJI	CCNNGG	4 cut(s)	19, 51, 606, 1244
BsaWI	WCCGGW	1 cut(s)	556
BsaXI	ACNNNNNCTCC	2 cut(s)	803, 833
Bsc4I	CCNNNNNNNGG	2 cut(s)	170, 349
Bse1I	ACTGG	2 cut(s)	673, 1165
Bse3DI	GCAATG	1 cut(s)	411
BseBI	CCWGG	2 cut(s)	52, 723
BseDI	CCNNGG	4 cut(s)	19, 51, 606, 1244
BseGI	GGATG	3 cut(s)	358, 585, 1282
BseLI	CCNNNNNNNGG	2 cut(s)	170, 349
BseMI	GCAATG	1 cut(s)	411
BseMII	CTCAG	3 cut(s)	520, 930, 1336
BseNI	ACTGG	2 cut(s)	673, 1165
BseRI	GAGGAG	1 cut(s)	792
BseXI	GCAGC	5 cut(s)	75, 161, 172, 585, 1094
Bsh1236I	CGCG	2 cut(s)	109, 425
Bsh1285I	CGRYCG	1 cut(s)	94
BshFI	GGCC	2 cut(s)	220, 514
BsiEI	CGRYCG	1 cut(s)	94
BsiHKAI	GWGCWC	1 cut(s)	214
BsiHKCI	CYCGRG	1 cut(s)	847
BsiSI	CCGG	2 cut(s)	120, 557
BslFI	GGGAC	3 cut(s)	68, 875, 1342
BslI	CCNNNNNNNGG	2 cut(s)	170, 349
BsmAI	GTCTC	3 cut(s)	428, 804, 850
BsmFI	GGGAC	3 cut(s)	68, 875, 1342
BsnI	GGCC	2 cut(s)	220, 514
Bso31I	GGTCTC	1 cut(s)	804
BsoBI	CYCGRG	1 cut(s)	847
Bsp1286I	GDGCHC	1 cut(s)	214
Bsp143I	GATC	1 cut(s)	955
Bsp19I	CCATGG	1 cut(s)	606
BspACI	CCGC	5 cut(s)	117, 511, 577, 1178, 1326
BspANI	GGCC	2 cut(s)	220, 514
BspCNI	CTCAG	3 cut(s)	519, 931, 1335
BspFNI	CGCG	2 cut(s)	109, 425
BspLI	GGNNCC	2 cut(s)	187, 1330
BspMAI	CTGCAG	1 cut(s)	162
BspTI	CTTAAG	1 cut(s)	470
BspTNI	GGTCTC	1 cut(s)	804
BsrBI	CCGCTC	1 cut(s)	1180
BsrDI	GCAATG	1 cut(s)	411
BsrI	ACTGG	2 cut(s)	673, 1165
BssECI	CCNNGG	4 cut(s)	19, 51, 606, 1244
BssMI	GATC	1 cut(s)	955
BssT1I	CCWWGG	2 cut(s)	606, 1244
Bst2UI	CCWGG	2 cut(s)	52, 723
Bst4CI	ACNGT	3 cut(s)	59, 95, 447
BstAFI	CTTAAG	1 cut(s)	470
BstBAI	YACGTR	2 cut(s)	1101, 1253
BstC8I	GCNNGC	4 cut(s)	913, 1182, 1193, 1229
BstDEI	CTNAG	3 cut(s)	506, 939, 1322
BstDSI	CCRYGG	2 cut(s)	19, 606
BstF5I	GGATG	3 cut(s)	358, 585, 1282
BstFNI	CGCG	2 cut(s)	109, 425
BstHHI	GCGC	4 cut(s)	111, 337, 383, 425
BstKTI	GATC	1 cut(s)	958
BstMAI	GTCTC	3 cut(s)	428, 804, 850
BstMBI	GATC	1 cut(s)	955
BstMCI	CGRYCG	1 cut(s)	94
BstMWI	GCNNNNNNNGC	4 cut(s)	413, 422, 475, 1151
BstNI	CCWGG	2 cut(s)	52, 723
BstNSI	RCATGY	2 cut(s)	1191, 1195
BstSCI	CCNGG	2 cut(s)	50, 721
BstSFI	CTRYAG	2 cut(s)	158, 981
BstUI	CGCG	2 cut(s)	109, 425
BstV1I	GCAGC	5 cut(s)	75, 161, 172, 585, 1094
BstX2I	RGATCY	1 cut(s)	955
BstYI	RGATCY	1 cut(s)	955
BsuI	GTATCC	2 cut(s)	577, 1090
BsuRI	GGCC	2 cut(s)	220, 514
BtgI	CCRYGG	2 cut(s)	19, 606
BtgZI	GCGATG	1 cut(s)	39
BtsCI	GGATG	3 cut(s)	358, 585, 1282
BtsIMutI	CAGTG	1 cut(s)	443
Cac8I	GCNNGC	4 cut(s)	913, 1182, 1193, 1229
CfoI	GCGC	4 cut(s)	111, 337, 383, 425
Cfr13I	GGNCC	2 cut(s)	218, 1329
CseI	GACGC	2 cut(s)	55, 924
Csp6I	GTAC	6 cut(s)	500, 541, 696, 1265, 1315, 1341
CviAII	CATG	7 cut(s)	607, 649, 874, 1031, 1188, 1192, 1287
CviQI	GTAC	6 cut(s)	500, 541, 696, 1265, 1315, 1341
DdeI	CTNAG	3 cut(s)	506, 939, 1322
DpnI	GATC	1 cut(s)	957
DpnII	GATC	1 cut(s)	955
DrdI	GACNNNNNNGTC	1 cut(s)	835
DseDI	GACNNNNNNGTC	1 cut(s)	835
EciI	GGCGGA	1 cut(s)	106
Eco130I	CCWWGG	2 cut(s)	606, 1244
Eco31I	GGTCTC	1 cut(s)	804
Eco32I	GATATC	1 cut(s)	951
Eco47I	GGWCC	1 cut(s)	1329
Eco57I	CTGAAG	2 cut(s)	27, 1157
Eco88I	CYCGRG	1 cut(s)	847
EcoO109I	RGGNCCY	1 cut(s)	218
EcoRII	CCWGG	2 cut(s)	50, 721
EcoRV	GATATC	1 cut(s)	951
EcoT14I	CCWWGG	2 cut(s)	606, 1244
EcoT22I	ATGCAT	1 cut(s)	1193
ErhI	CCWWGG	2 cut(s)	606, 1244
FaeI	CATG	7 cut(s)	610, 652, 877, 1034, 1191, 1195, 1290
FalI	AAGNNNNNCTT	2 cut(s)	915, 947
FaqI	GGGAC	3 cut(s)	68, 875, 1342
FatI	CATG	7 cut(s)	606, 648, 873, 1030, 1187, 1191, 1286
FauI	CCCGC	1 cut(s)	1319
FblI	GTMKAC	1 cut(s)	645
Fnu4HI	GCNGC	7 cut(s)	64, 161, 175, 512, 599, 1083, 1178
FokI	GGATG	3 cut(s)	365, 592, 1269
Fsp4HI	GCNGC	7 cut(s)	64, 161, 175, 512, 599, 1083, 1178
FspBI	CTAG	5 cut(s)	42, 522, 1013, 1068, 1109
GlaI	GCGC	4 cut(s)	110, 336, 382, 424
GluI	GCNGC	7 cut(s)	64, 161, 175, 512, 599, 1083, 1178
HaeIII	GGCC	2 cut(s)	220, 514
HapII	CCGG	2 cut(s)	120, 557
HgaI	GACGC	2 cut(s)	55, 924
HhaI	GCGC	4 cut(s)	111, 337, 383, 425
Hin1II	CATG	7 cut(s)	610, 652, 877, 1034, 1191, 1195, 1290
Hin6I	GCGC	4 cut(s)	109, 335, 381, 423
HinP1I	GCGC	4 cut(s)	109, 335, 381, 423
HincII	GTYRAC	1 cut(s)	646
HindII	GTYRAC	1 cut(s)	646
HinfI	GANTC	7 cut(s)	45, 198, 570, 850, 863, 884, 1105
HpaII	CCGG	2 cut(s)	120, 557
HphI	GGTGA	4 cut(s)	40, 401, 723, 1154
Hpy166II	GTNNAC	1 cut(s)	646
Hpy188I	TCNGA	5 cut(s)	7, 631, 940, 955, 1240
Hpy188III	TCNNGA	6 cut(s)	231, 266, 826, 847, 965, 1275
Hpy8I	GTNNAC	1 cut(s)	646
Hpy99I	CGWCG	3 cut(s)	89, 375, 430
HpyAV	CCTTC	2 cut(s)	190, 245
HpyCH4III	ACNGT	3 cut(s)	59, 95, 447
HpyCH4IV	ACGT	4 cut(s)	428, 587, 1100, 1252
HpyCH4V	TGCA	9 cut(s)	63, 160, 711, 783, 1007, 1191, 1195, 1231, 1294
HpyF10VI	GCNNNNNNNGC	4 cut(s)	413, 422, 475, 1151
HpyF3I	CTNAG	3 cut(s)	506, 939, 1322
HpySE526I	ACGT	4 cut(s)	428, 587, 1100, 1252
Hsp92II	CATG	7 cut(s)	610, 652, 877, 1034, 1191, 1195, 1290
HspAI	GCGC	4 cut(s)	109, 335, 381, 423
Kzo9I	GATC	1 cut(s)	955
LmnI	GCTCC	6 cut(s)	21, 122, 158, 209, 496, 1150
Lsp1109I	GCAGC	5 cut(s)	75, 161, 172, 585, 1094
LweI	GCATC	3 cut(s)	16, 343, 357
MaeI	CTAG	5 cut(s)	42, 522, 1013, 1068, 1109
MaeII	ACGT	4 cut(s)	428, 587, 1100, 1252
MaeIII	GTNAC	2 cut(s)	851, 1248
MalI	GATC	1 cut(s)	957
MbiI	CCGCTC	1 cut(s)	1180
MboI	GATC	1 cut(s)	955
MboII	GAAGA	6 cut(s)	20, 559, 785, 894, 932, 1163
MflI	RGATCY	1 cut(s)	955
MhlI	GDGCHC	1 cut(s)	214
MluCI	AATT	9 cut(s)	128, 135, 148, 564, 625, 777, 879, 1138, 1233
MlyI	GAGTC	5 cut(s)	39, 207, 857, 859, 1114
MmeI	TCCRAC	1 cut(s)	634
MnlI	CCTC	9 cut(s)	122, 253, 529, 650, 686, 747, 780, 789, 813
Mph1103I	ATGCAT	1 cut(s)	1193
MseI	TTAA	4 cut(s)	299, 471, 1073, 1113
MslI	CAYNNNNRTG	1 cut(s)	24
MspA1I	CMGCKG	1 cut(s)	1326
MspCI	CTTAAG	1 cut(s)	470
MspI	CCGG	2 cut(s)	120, 557
MspR9I	CCNGG	2 cut(s)	52, 723
MvaI	CCWGG	2 cut(s)	52, 723
MvnI	CGCG	2 cut(s)	109, 425
MwoI	GCNNNNNNNGC	4 cut(s)	413, 422, 475, 1151
NcoI	CCATGG	1 cut(s)	606
NdeII	GATC	1 cut(s)	955
NlaIII	CATG	7 cut(s)	610, 652, 877, 1034, 1191, 1195, 1290
NlaIV	GGNNCC	2 cut(s)	187, 1330
NmuCI	GTSAC	1 cut(s)	851
NsiI	ATGCAT	1 cut(s)	1193
NspI	RCATGY	2 cut(s)	1191, 1195
PaeI	GCATGC	1 cut(s)	1195
PaeR7I	CTCGAG	1 cut(s)	847
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PfeI	GAWTC	2 cut(s)	570, 884
PkrI	GCNGC	7 cut(s)	65, 162, 176, 513, 600, 1084, 1179
PleI	GAGTC	5 cut(s)	39, 206, 857, 858, 1113
PpsI	GAGTC	5 cut(s)	39, 206, 857, 858, 1113
Ppu21I	YACGTR	2 cut(s)	1101, 1253
Psp6I	CCWGG	2 cut(s)	50, 721
PspGI	CCWGG	2 cut(s)	50, 721
PspN4I	GGNNCC	2 cut(s)	187, 1330
PspPI	GGNCC	2 cut(s)	218, 1329
PsrI	GAACNNNNNNTAC	2 cut(s)	1233, 1265
PstI	CTGCAG	1 cut(s)	162
PsuI	RGATCY	1 cut(s)	955
RsaI	GTAC	6 cut(s)	501, 542, 697, 1266, 1316, 1342
RsaNI	GTAC	6 cut(s)	500, 541, 696, 1265, 1315, 1341
RseI	CAYNNNNRTG	1 cut(s)	24
SalI	GTCGAC	1 cut(s)	644
SaqAI	TTAA	4 cut(s)	299, 471, 1073, 1113
SatI	GCNGC	7 cut(s)	64, 161, 175, 512, 599, 1083, 1178
Sau3AI	GATC	1 cut(s)	955
Sau96I	GGNCC	2 cut(s)	218, 1329
SchI	GAGTC	5 cut(s)	39, 207, 857, 859, 1114
ScrFI	CCNGG	2 cut(s)	52, 723
SduI	GDGCHC	1 cut(s)	214
SfaNI	GCATC	3 cut(s)	16, 343, 357
SfcI	CTRYAG	2 cut(s)	158, 981
Sfr274I	CTCGAG	1 cut(s)	847
SinI	GGWCC	1 cut(s)	1329
SlaI	CTCGAG	1 cut(s)	847
SmiMI	CAYNNNNRTG	1 cut(s)	24
SmlI	CTYRAG	2 cut(s)	470, 847
SmoI	CTYRAG	2 cut(s)	470, 847
SpeI	ACTAGT	1 cut(s)	1067
SphI	GCATGC	1 cut(s)	1195
Sse9I	AATT	9 cut(s)	128, 135, 148, 564, 625, 777, 879, 1138, 1233
SsiI	CCGC	5 cut(s)	117, 511, 577, 1178, 1326
SspMI	CTAG	5 cut(s)	42, 522, 1013, 1068, 1109
StyD4I	CCNGG	2 cut(s)	50, 721
StyI	CCWWGG	2 cut(s)	606, 1244
TaaI	ACNGT	3 cut(s)	59, 95, 447
TaiI	ACGT	4 cut(s)	431, 590, 1103, 1255
TaqI	TCGA	6 cut(s)	196, 201, 287, 310, 645, 848
TasI	AATT	9 cut(s)	128, 135, 148, 564, 625, 777, 879, 1138, 1233
TatI	WGTACW	1 cut(s)	499
TauI	GCSGC	2 cut(s)	514, 1180
TfiI	GAWTC	2 cut(s)	570, 884
Tru1I	TTAA	4 cut(s)	299, 471, 1073, 1113
Tru9I	TTAA	4 cut(s)	299, 471, 1073, 1113
TscAI	CASTG	1 cut(s)	450
TseFI	GTSAC	1 cut(s)	851
TseI	GCWGC	5 cut(s)	63, 160, 174, 598, 1082
Tsp45I	GTSAC	1 cut(s)	851
TspDTI	ATGAA	5 cut(s)	264, 408, 906, 1047, 1313
TspGWI	ACGGA	3 cut(s)	222, 811, 932
TspRI	CASTG	1 cut(s)	450
Vha464I	CTTAAG	1 cut(s)	470
VpaK11BI	GGWCC	1 cut(s)	1329
XapI	RAATTY	3 cut(s)	777, 1138, 1233
XceI	RCATGY	2 cut(s)	1191, 1195
XhoI	CTCGAG	1 cut(s)	847
XmiI	GTMKAC	1 cut(s)	645
XspI	CTAG	5 cut(s)	42, 522, 1013, 1068, 1109
Zsp2I	ATGCAT	1 cut(s)	1193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.