MD15G1034200.v1.1

B3 domain-containing protein Os01g0723500-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011529
Physical Location & Seq
Reverse (-)
2413586 .. 2417680
4095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1034200.v1.1.491

Sequence Viewer

Length: 1698 bp
ATGGCAGAGGAAGCTCGGAATGTGAGGAGCCCTCATTTTTTTGCGTTTTATTCTGCTGATTTGAGCTCTGAAAGACTGAAAATCCCAGAAAGATTCATGAGACACATGGAAGGCAGAACATCTGGGTTAGTTTCGTTGTTCGGCCCTAGCGGCAGTGAATGGAGTGTTGAATTGATTCAACAGAATGGTGGTTTGTTCTTGCATCGTGGGTGGCCGGCATTTGTGAGAGACCATTATGTGGAATGCGGGGACTTCTTGATTTTTAGATACGATGGTGATTTGTGTTTCACCGTGCTAATTTTTGATCAAAGTGCATGTGAGAAAGAGGCTGCATTTCGTTTTGGATACGGGCAAGACTCCAGGAACTTTGACAAGTATATGTATATGGGACGAAAAAGGAGAAGGGAGGAGGTCGCATCATCTGGGAATAAGCTGGTTGATGGTGCGGTGAAGAAGATTAGAAACGGTTCGTCTCAATTCCGGTCAGAATGCATTGATGAGGGTCGAGAAGAAGCAACTTGTTCCACAGAAAAAAATCCACTTGAGAATCCTGGTTTGGATGTTGTTTCATATAAAGCGAAGGAATCGATCCAAAATATGTGTGGAAAAGGAGATGGATTAAATGTTAATGGTAGACTTTGTATGCAGATGTCATCAGCACATGAAGTAGCTCCATCATTTAGCTCCTCTAATCCTTATTTCGTGAGAATCATAAAAAGTTTCAACATCAGTGGTTCATATACTCTGAATATCCCATATAAATTTTCAGTGGCACATTTACCAAACTGCAAAGTAAAGATTGTCCTTCAAAATTGGAAAGGAGAAAGTTGGACTGTCAATTCTGTGCCAAGTAATAGAGTGCATACCAGTCACACTCTTTGTGGAGGATGGATGGCGTTTGTTCGTTATAATGACATACAGCTGGGAGATATCTGCACTTTTGAACTTGTGCGTGAGTTTGAATTCTGTGTCCACATTCAAAAGGGAGAAACATCTAGTTGGTTAAGTCCGGGGCTAGCTGTCACTACGCATAAAACTATCAAAGGCTTGTCAAAGAAAATGAAAGGGAACTCTCCCAAAGTCCTTTCAAAGAGCATAAGAGAGATAGCTGATAAACTATCATCAAAGAAGGATCAAGACGCTGCTTTCATTAATGACACAAGGAAATATGGCAGCACATCAAGATCTCTTACTAAAGCTGCACTCGCTCAGTCAAAAGTTGCTAGTAAAAAGTTGGTTAATCGCAGGAAAAAAGTTGTTGATGATGAACTGGATTCAAAATCAAGTGGTTTGAGAATCAAGTTAACACTTGATGAAGAAAGAATAGCTCGATCTTTCACTTCTTGTTTCCCGAATTTTGTGAAAATCATGAAGAAGTTCAACATCAGTGGATCTTATACATTGAAAATCCCATACCAGTTTTCCACAGAATACCTCCCCAACTTTAAAACAGAGATTGTGCTTCGAAACTCAAAGGGGGAATGTTGGACTGTGAACTCTGTCCCAGACTCGAAAGGGCGTGTGGTGCATACTTTTTGCGGAGGGTGGATGGCATTTGTACGCGGAAATGACATTAACATTGGAGATGTTTGCATTTTTGAGCTTGTTGGTAAGGATGAAATGCACGTGCACATTTCCGGTGTTGGTAAAAAGGGATTTGACCATCTAGGCGGACAAGGAACATCAAATGTAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

566

Amino Acids

63.46

Weight (kDa)

9.22

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 18 - 103 3e-16 B3 DNA binding domain
B3 PF02362 247 - 328 4.9e-13 B3 DNA binding domain
B3 PF02362 453 - 545 1.4e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
AccB7I CCANNNNNTGG 1 cut(s) 238
AccI GTMKAC 1 cut(s) 634
AccII CGCG 1 cut(s) 1563
AciI CCGC 6 cut(s) 150, 246, 446, 1539, 1563, 1671
AclWI GGATC 3 cut(s) 583, 1140, 1399
AcoI YGGCCR 1 cut(s) 212
AcsI RAATTY 3 cut(s) 761, 962, 1354
AcvI CACGTG 1 cut(s) 1627
AfaI GTAC 1 cut(s) 1560
AfiI CCNNNNNNNGG 1 cut(s) 238
AjnI CCWGG 2 cut(s) 359, 550
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
Alw21I GWGCWC 2 cut(s) 68, 1632
Alw26I GTCTC 3 cut(s) 94, 222, 477
Alw44I GTGCAC 1 cut(s) 1628
AlwI GGATC 3 cut(s) 583, 1140, 1399
AoxI GGCC 2 cut(s) 142, 212
ApaLI GTGCAC 1 cut(s) 1628
ApeKI GCWGC 4 cut(s) 329, 1142, 1173, 1199
ApoI RAATTY 3 cut(s) 761, 962, 1354
AseI ATTAAT 1 cut(s) 1152
Asp700I GAANNNNTTC 3 cut(s) 174, 466, 1376
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 1011
AsuHPI GGTGA 3 cut(s) 280, 287, 460
AsuII TTCGAA 1 cut(s) 1465
AsuNHI GCTAGC 1 cut(s) 1015
BaeGI GKGCMC 1 cut(s) 1632
BaeI ACNNNNGTAYC 2 cut(s) 259, 292
BanII GRGCYC 2 cut(s) 32, 68
BbrPI CACGTG 1 cut(s) 1627
Bbv12I GWGCWC 2 cut(s) 68, 1632
BbvI GCAGC 4 cut(s) 316, 1129, 1185, 1186
BccI CCATC 8 cut(s) 266, 434, 608, 682, 882, 886, 1543, 1671
BciT130I CCWGG 2 cut(s) 361, 552
BciVI GTATCC 1 cut(s) 338
BclI TGATCA 1 cut(s) 304
BcnI CCSGG 1 cut(s) 1011
BcoDI GTCTC 3 cut(s) 94, 222, 477
BfaI CTAG 5 cut(s) 147, 996, 1016, 1224, 1667
BfuI GTATCC 1 cut(s) 338
BglI GCCNNNNNGGC 1 cut(s) 150
BglII AGATCT 1 cut(s) 1184
BisI GCNGC 5 cut(s) 151, 330, 1143, 1174, 1200
BlsI GCNGC 5 cut(s) 152, 331, 1144, 1175, 1201
Bme1390I CCNGG 3 cut(s) 361, 552, 1011
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 1 cut(s) 29
BmrFI CCNGG 3 cut(s) 361, 552, 1011
BmsI GCATC 2 cut(s) 211, 425
BmtI GCTAGC 1 cut(s) 1019
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpmI CTGGAG 1 cut(s) 343
Bpu14I TTCGAA 1 cut(s) 1465
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 1011
Bsa29I ATCGAT 1 cut(s) 587
BsaAI YACGTR 1 cut(s) 1627
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 1010
BsaWI WCCGGW 2 cut(s) 480, 1637
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 3 cut(s) 867, 1275, 1417
BseBI CCWGG 2 cut(s) 361, 552
BseCI ATCGAT 1 cut(s) 587
BseDI CCNNGG 1 cut(s) 1010
BseGI GGATG 5 cut(s) 565, 893, 897, 1554, 1621
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMII CTCAG 1 cut(s) 1223
BseNI ACTGG 3 cut(s) 867, 1275, 1417
BseRI GAGGAG 3 cut(s) 40, 422, 676
BseSI GKGCMC 1 cut(s) 1632
BseXI GCAGC 4 cut(s) 316, 1129, 1185, 1186
BseYI CCCAGC 1 cut(s) 922
BsgI GTGCAG 2 cut(s) 919, 1185
Bsh1236I CGCG 1 cut(s) 1563
BshFI GGCC 2 cut(s) 144, 214
BshVI ATCGAT 1 cut(s) 587
BsiHKAI GWGCWC 2 cut(s) 68, 1632
BsiSI CCGG 4 cut(s) 215, 481, 1010, 1638
BslFI GGGAC 3 cut(s) 263, 402, 1487
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 3 cut(s) 94, 222, 477
BsmBI CGTCTC 1 cut(s) 477
BsmFI GGGAC 3 cut(s) 263, 402, 1487
BsmI GAATGC 2 cut(s) 248, 494
BsnI GGCC 2 cut(s) 144, 214
Bso31I GGTCTC 1 cut(s) 222
Bsp119I TTCGAA 1 cut(s) 1465
Bsp1286I GDGCHC 3 cut(s) 32, 68, 1632
Bsp143I GATC 6 cut(s) 304, 588, 1132, 1184, 1331, 1391
BspACI CCGC 6 cut(s) 150, 246, 446, 1539, 1563, 1671
BspANI GGCC 2 cut(s) 144, 214
BspCNI CTCAG 1 cut(s) 1222
BspDI ATCGAT 1 cut(s) 587
BspFNI CGCG 1 cut(s) 1563
BspHI TCATGA 2 cut(s) 96, 1368
BspLI GGNNCC 1 cut(s) 29
BspOI GCTAGC 1 cut(s) 1019
BspPI GGATC 3 cut(s) 583, 1140, 1399
BspT104I TTCGAA 1 cut(s) 1465
BspTNI GGTCTC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 3 cut(s) 867, 1275, 1417
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 1010
BssMI GATC 6 cut(s) 304, 588, 1132, 1184, 1331, 1391
Bst2UI CCWGG 2 cut(s) 361, 552
Bst4CI ACNGT 4 cut(s) 292, 467, 835, 1492
BstBAI YACGTR 1 cut(s) 1627
BstBI TTCGAA 1 cut(s) 1465
BstC8I GCNNGC 2 cut(s) 216, 1017
BstDEI CTNAG 1 cut(s) 1209
BstF5I GGATG 5 cut(s) 565, 893, 897, 1554, 1621
BstFNI CGCG 1 cut(s) 1563
BstKTI GATC 6 cut(s) 307, 591, 1135, 1187, 1334, 1394
BstMAI GTCTC 3 cut(s) 94, 222, 477
BstMBI GATC 6 cut(s) 304, 588, 1132, 1184, 1331, 1391
BstMWI GCNNNNNNNGC 4 cut(s) 11, 150, 1205, 1525
BstNI CCWGG 2 cut(s) 361, 552
BstNSI RCATGY 1 cut(s) 318
BstSCI CCNGG 3 cut(s) 359, 550, 1009
BstSLI GKGCMC 1 cut(s) 1632
BstUI CGCG 1 cut(s) 1563
BstV1I GCAGC 4 cut(s) 316, 1129, 1185, 1186
BstX2I RGATCY 2 cut(s) 1184, 1391
BstYI RGATCY 2 cut(s) 1184, 1391
Bsu15I ATCGAT 1 cut(s) 587
BsuI GTATCC 1 cut(s) 338
BsuRI GGCC 2 cut(s) 144, 214
BsuTUI ATCGAT 1 cut(s) 587
BtsCI GGATG 5 cut(s) 565, 893, 897, 1554, 1621
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 4 cut(s) 160, 736, 774, 1393
Cac8I GCNNGC 2 cut(s) 216, 1017
CciI TCATGA 2 cut(s) 96, 1368
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 143
ClaI ATCGAT 1 cut(s) 587
CseI GACGC 1 cut(s) 1148
Csp6I GTAC 1 cut(s) 1559
CspCI CAANNNNNGTGG 4 cut(s) 712, 747, 1369, 1404
CviAII CATG 5 cut(s) 97, 106, 315, 662, 1369
CviQI GTAC 1 cut(s) 1559
DdeI CTNAG 1 cut(s) 1209
DpnI GATC 6 cut(s) 306, 590, 1134, 1186, 1333, 1393
DpnII GATC 6 cut(s) 304, 588, 1132, 1184, 1331, 1391
DraI TTTAAA 1 cut(s) 1447
EaeI YGGCCR 1 cut(s) 212
EciI GGCGGA 1 cut(s) 1686
Ecl136II GAGCTC 1 cut(s) 66
Eco24I GRGCYC 2 cut(s) 32, 68
Eco31I GGTCTC 1 cut(s) 222
Eco32I GATATC 1 cut(s) 931
Eco53kI GAGCTC 1 cut(s) 66
Eco72I CACGTG 1 cut(s) 1627
EcoICRI GAGCTC 1 cut(s) 66
EcoRI GAATTC 1 cut(s) 962
EcoRII CCWGG 2 cut(s) 359, 550
EcoRV GATATC 1 cut(s) 931
EcoT22I ATGCAT 1 cut(s) 494
EcoT38I GRGCYC 2 cut(s) 32, 68
Esp3I CGTCTC 1 cut(s) 477
FaeI CATG 5 cut(s) 100, 109, 318, 665, 1372
FaqI GGGAC 3 cut(s) 263, 402, 1487
FatI CATG 5 cut(s) 96, 105, 314, 661, 1368
FauI CCCGC 1 cut(s) 239
FbaI TGATCA 1 cut(s) 304
FblI GTMKAC 1 cut(s) 634
Fnu4HI GCNGC 5 cut(s) 151, 330, 1143, 1174, 1200
FokI GGATG 5 cut(s) 572, 900, 904, 1561, 1628
FriOI GRGCYC 2 cut(s) 32, 68
Fsp4HI GCNGC 5 cut(s) 151, 330, 1143, 1174, 1200
FspBI CTAG 5 cut(s) 147, 996, 1016, 1224, 1667
GluI GCNGC 5 cut(s) 151, 330, 1143, 1174, 1200
GsaI CCCAGC 1 cut(s) 926
GsuI CTGGAG 1 cut(s) 343
HaeIII GGCC 2 cut(s) 144, 214
HapII CCGG 4 cut(s) 215, 481, 1010, 1638
HgaI GACGC 1 cut(s) 1148
Hin1II CATG 5 cut(s) 100, 109, 318, 665, 1372
HincII GTYRAC 1 cut(s) 1305
HindII GTYRAC 1 cut(s) 1305
HinfI GANTC 9 cut(s) 93, 175, 356, 547, 584, 708, 1274, 1296, 1508
HpaI GTTAAC 1 cut(s) 1305
HpaII CCGG 4 cut(s) 215, 481, 1010, 1638
HphI GGTGA 3 cut(s) 280, 287, 460
Hpy166II GTNNAC 5 cut(s) 635, 973, 1305, 1495, 1630
Hpy188I TCNGA 4 cut(s) 18, 70, 487, 747
Hpy188III TCNNGA 8 cut(s) 97, 256, 506, 703, 1136, 1182, 1351, 1369
Hpy8I GTNNAC 5 cut(s) 635, 973, 1305, 1495, 1630
HpyAV CCTTC 5 cut(s) 104, 396, 574, 815, 1123
HpyCH4III ACNGT 4 cut(s) 292, 467, 835, 1492
HpyCH4IV ACGT 1 cut(s) 1626
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 150, 1205, 1525
HpyF3I CTNAG 1 cut(s) 1209
HpySE526I ACGT 1 cut(s) 1626
Hsp92II CATG 5 cut(s) 100, 109, 318, 665, 1372
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 1305
Kzo9I GATC 6 cut(s) 304, 588, 1132, 1184, 1331, 1391
LmnI GCTCC 3 cut(s) 27, 676, 689
Lsp1109I GCAGC 4 cut(s) 316, 1129, 1185, 1186
LweI GCATC 2 cut(s) 211, 425
MaeI CTAG 5 cut(s) 147, 996, 1016, 1224, 1667
MaeII ACGT 1 cut(s) 1626
MaeIII GTNAC 2 cut(s) 869, 1021
MalI GATC 6 cut(s) 306, 590, 1134, 1186, 1333, 1393
MboI GATC 6 cut(s) 304, 588, 1132, 1184, 1331, 1391
MboII GAAGA 5 cut(s) 463, 466, 521, 1328, 1384
MflI RGATCY 2 cut(s) 1184, 1391
MhlI GDGCHC 3 cut(s) 32, 68, 1632
MluCI AATT 8 cut(s) 170, 297, 476, 761, 811, 838, 962, 1354
MlyI GAGTC 2 cut(s) 350, 1502
MmeI TCCRAC 2 cut(s) 809, 1466
Mph1103I ATGCAT 1 cut(s) 494
MroNI GCCGGC 1 cut(s) 214
MroXI GAANNNNTTC 3 cut(s) 174, 466, 1376
MseI TTAA 8 cut(s) 620, 627, 1004, 1152, 1239, 1304, 1446, 1575
MspA1I CMGCKG 1 cut(s) 922
MspI CCGG 4 cut(s) 215, 481, 1010, 1638
MspR9I CCNGG 3 cut(s) 361, 552, 1011
Mva1269I GAATGC 2 cut(s) 248, 494
MvaI CCWGG 2 cut(s) 361, 552
MvnI CGCG 1 cut(s) 1563
MwoI GCNNNNNNNGC 4 cut(s) 11, 150, 1205, 1525
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 1011
NdeII GATC 6 cut(s) 304, 588, 1132, 1184, 1331, 1391
NgoMIV GCCGGC 1 cut(s) 214
NheI GCTAGC 1 cut(s) 1015
NlaIII CATG 5 cut(s) 100, 109, 318, 665, 1372
NlaIV GGNNCC 1 cut(s) 29
NmuCI GTSAC 2 cut(s) 869, 1021
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 1 cut(s) 318
NspV TTCGAA 1 cut(s) 1465
PagI TCATGA 2 cut(s) 96, 1368
PctI GAATGC 2 cut(s) 248, 494
PdiI GCCGGC 1 cut(s) 216
PdmI GAANNNNTTC 3 cut(s) 174, 466, 1376
PfeI GAWTC 7 cut(s) 93, 175, 547, 584, 708, 1274, 1296
PflMI CCANNNNNTGG 1 cut(s) 238
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 5 cut(s) 152, 331, 1144, 1175, 1201
PleI GAGTC 2 cut(s) 350, 1502
PmaCI CACGTG 1 cut(s) 1627
PmlI CACGTG 1 cut(s) 1627
PpsI GAGTC 2 cut(s) 350, 1502
Ppu21I YACGTR 1 cut(s) 1627
PshBI ATTAAT 1 cut(s) 1152
PsiI TTATAA 1 cut(s) 909
Psp124BI GAGCTC 1 cut(s) 68
Psp6I CCWGG 2 cut(s) 359, 550
PspCI CACGTG 1 cut(s) 1627
PspFI CCCAGC 1 cut(s) 922
PspGI CCWGG 2 cut(s) 359, 550
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 143
PsuI RGATCY 2 cut(s) 1184, 1391
PvuII CAGCTG 1 cut(s) 922
RsaI GTAC 1 cut(s) 1560
RsaNI GTAC 1 cut(s) 1559
SacI GAGCTC 1 cut(s) 68
SaqAI TTAA 8 cut(s) 620, 627, 1004, 1152, 1239, 1304, 1446, 1575
SatI GCNGC 5 cut(s) 151, 330, 1143, 1174, 1200
Sau3AI GATC 6 cut(s) 304, 588, 1132, 1184, 1331, 1391
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 2 cut(s) 350, 1502
ScrFI CCNGG 3 cut(s) 361, 552, 1011
SduI GDGCHC 3 cut(s) 32, 68, 1632
SfaNI GCATC 2 cut(s) 211, 425
SfuI TTCGAA 1 cut(s) 1465
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 8 cut(s) 170, 297, 476, 761, 811, 838, 962, 1354
SsiI CCGC 6 cut(s) 150, 246, 446, 1539, 1563, 1671
SspMI CTAG 5 cut(s) 147, 996, 1016, 1224, 1667
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 3 cut(s) 359, 550, 1009
TaaI ACNGT 4 cut(s) 292, 467, 835, 1492
TaiI ACGT 1 cut(s) 1629
TaqI TCGA 5 cut(s) 505, 587, 1330, 1465, 1511
TasI AATT 8 cut(s) 170, 297, 476, 761, 811, 838, 962, 1354
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 7 cut(s) 93, 175, 547, 584, 708, 1274, 1296
Tru1I TTAA 8 cut(s) 620, 627, 1004, 1152, 1239, 1304, 1446, 1575
Tru9I TTAA 8 cut(s) 620, 627, 1004, 1152, 1239, 1304, 1446, 1575
TscAI CASTG 4 cut(s) 160, 736, 774, 1393
TseFI GTSAC 2 cut(s) 869, 1021
TseI GCWGC 4 cut(s) 329, 1142, 1173, 1199
Tsp45I GTSAC 2 cut(s) 869, 1021
TspRI CASTG 4 cut(s) 160, 736, 774, 1393
Van91I CCANNNNNTGG 1 cut(s) 238
VneI GTGCAC 1 cut(s) 1628
VspI ATTAAT 1 cut(s) 1152
XapI RAATTY 3 cut(s) 761, 962, 1354
XceI RCATGY 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XmiI GTMKAC 1 cut(s) 634
XmnI GAANNNNTTC 3 cut(s) 174, 466, 1376
XspI CTAG 5 cut(s) 147, 996, 1016, 1224, 1667
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.