Rh6CG420700

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0011529
Physical Location & Seq
Forward (+)
60334157 .. 60337927
3771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG420700.1

Sequence Viewer

Length: 1107 bp
ATGGCGGAAGGAGCTTTAAAGGTGATGCCTAATGCGAGAAGGCCTCACTTCTTTGCCTTCTTTTCTGCTGATTTGAGCTCAGAGCGACTGAAAATACCAGAGAGGTTTATGATTCACATGGAAGGGAGAACATCTGGAATAGTTGTGTTGTCTGGTCCTAGCGGGAATGCATGGAATGTTCACTTGATTGAACAAAATGGTGATTTGTTCTTCCATTATGGGTGGCCGGCATTTGTAAGAGACCATTCCCTAGAATGTGGTGACTTCTTGATTTTCAGATATGATGGTGAGTTGCGTTTCACTGTGCAAATTTTTGATCAAACTGCGTGTGAGAAAGAAGCCGCATTTCATTCCCAATGTGGCCAAAGTTCTAGCTACTTTGAAAAACTTATTGGACGGAAGAGGGCCAGGGAGGAAGGATGTGGGCTTGACAGGCTGCTATTTGAAGAATCGACTTCTTCCCAGCAATCAAAGACATATGAAAATCCTGGTTTGGATGTTGTCATATACAAAGCAAAGCAAGCCATCCAGAATAATTTGAATGATAGAGTTTGTATGCAGATATCTTCGGCACATGAAGTAGCTCAATCATTTAGCTCCTCTAATCCTTATTTTCTGAGAATCATGAAAAGTTTCAACATCAGTGGTTCATATACTTTGAATATTCCATATAAATTTTCAGTGGCACATCTACCAAACTGTAAAGTAAAGATTGTCCTTCTAAACTTGAAAGGAGAAAGTTGGACCGTCAATTCTGTGCCGACCACAAGAGTGCATACAAGTCATACTCTTTGTGGAGGATGGATGGCTTTTGTCCGTTATAATGACATAAAGCTAGGAGATATCTGCATTTTTGAACTTGTGCGGGAGTGTGAATTTCGCGTCCATATTCAAACTGGAGAAACATCTAGTAGGTTATGTGCCGGGCTAGCTTTGAAAGGTTTGTCAAAGAAAGTGAAGGGGAACTCTCCTAAAGTCCATACAAAGAGCCTAAGGAAGATAGAGATGTCTGAAACAAGGAAACACGGAAGCACATCAAAATCTATTGCCAAAGGCGCACCTCAGTCAAAAGCAGCCAGTAAAAAGTTGGGTAAGGTTTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

368

Amino Acids

41.27

Weight (kDa)

9.37

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 18 - 107 3.5e-17 B3 DNA binding domain
B3 PF02362 212 - 297 2.1e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 822
AccII CGCG 1 cut(s) 882
AciI CCGC 4 cut(s) 5, 162, 342, 865
AcoI YGGCCR 2 cut(s) 224, 361
AcsI RAATTY 3 cut(s) 309, 674, 875
AjnI CCWGG 2 cut(s) 407, 487
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AleI CACNNNNGTG 1 cut(s) 770
AluBI AGCT 7 cut(s) 14, 78, 375, 584, 597, 835, 932
AluI AGCT 7 cut(s) 14, 78, 375, 584, 597, 835, 932
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 1 cut(s) 234
AoxI GGCC 4 cut(s) 41, 224, 361, 405
ApeKI GCWGC 2 cut(s) 436, 1073
ApoI RAATTY 3 cut(s) 309, 674, 875
Asp700I GAANNNNTTC 1 cut(s) 632
AspLEI GCGC 1 cut(s) 1058
AspS9I GGNCC 3 cut(s) 155, 405, 744
AsuC2I CCSGG 1 cut(s) 925
AsuHPI GGTGA 4 cut(s) 34, 212, 272, 299
AsuNHI GCTAGC 1 cut(s) 928
AvaII GGWCC 2 cut(s) 155, 744
AxyI CCTNAGG 1 cut(s) 992
BalI TGGCCA 1 cut(s) 363
BanII GRGCYC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 423, 1085
BccI CCATC 4 cut(s) 278, 533, 795, 799
BciT130I CCWGG 2 cut(s) 409, 489
BclI TGATCA 1 cut(s) 316
BcnI CCSGG 1 cut(s) 925
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 6 cut(s) 159, 251, 372, 836, 909, 929
BisI GCNGC 3 cut(s) 342, 437, 1074
BlsI GCNGC 3 cut(s) 343, 438, 1075
Bme1390I CCNGG 3 cut(s) 409, 489, 925
Bme18I GGWCC 2 cut(s) 155, 744
BmgT120I GGNCC 3 cut(s) 155, 405, 744
BmrFI CCNGG 3 cut(s) 409, 489, 925
BmsI GCATC 1 cut(s) 15
BmtI GCTAGC 1 cut(s) 932
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 918
BpuMI CCSGG 1 cut(s) 925
BsaI GGTCTC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 408
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 2 cut(s) 901, 1077
Bse21I CCTNAGG 1 cut(s) 992
BseBI CCWGG 2 cut(s) 409, 489
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 5 cut(s) 425, 502, 525, 806, 810
BseMII CTCAG 3 cut(s) 93, 608, 1076
BseNI ACTGG 2 cut(s) 901, 1077
BseRI GAGGAG 1 cut(s) 589
BseXI GCAGC 2 cut(s) 423, 1085
BseYI CCCAGC 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 882
BshFI GGCC 4 cut(s) 43, 226, 363, 407
BsiHKAI GWGCWC 1 cut(s) 80
BsiSI CCGG 2 cut(s) 227, 924
BsmAI GTCTC 1 cut(s) 234
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 4 cut(s) 43, 226, 363, 407
Bso31I GGTCTC 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 4 cut(s) 5, 162, 342, 865
BspANI GGCC 4 cut(s) 43, 226, 363, 407
BspCNI CTCAG 3 cut(s) 92, 609, 1075
BspFNI CGCG 1 cut(s) 882
BspHI TCATGA 1 cut(s) 624
BspOI GCTAGC 1 cut(s) 932
BspTNI GGTCTC 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 2 cut(s) 901, 1077
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 1 cut(s) 316
Bst2UI CCWGG 2 cut(s) 409, 489
Bst4CI ACNGT 3 cut(s) 304, 701, 748
Bst6I CTCTTC 1 cut(s) 395
BstC8I GCNNGC 3 cut(s) 228, 522, 930
BstDEI CTNAG 4 cut(s) 79, 617, 992, 1062
BstF5I GGATG 5 cut(s) 425, 502, 525, 806, 810
BstFNI CGCG 1 cut(s) 882
BstHHI GCGC 1 cut(s) 1058
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 5 cut(s) 11, 433, 521, 929, 1055
BstNI CCWGG 2 cut(s) 409, 489
BstSCI CCNGG 3 cut(s) 407, 487, 923
BstUI CGCG 1 cut(s) 882
BstV1I GCAGC 2 cut(s) 423, 1085
Bsu36I CCTNAGG 1 cut(s) 992
BsuRI GGCC 4 cut(s) 43, 226, 363, 407
BtsCI GGATG 5 cut(s) 425, 502, 525, 806, 810
BtsIMutI CAGTG 3 cut(s) 300, 649, 687
Cac8I GCNNGC 3 cut(s) 228, 522, 930
CciI TCATGA 1 cut(s) 624
CfoI GCGC 1 cut(s) 1058
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 3 cut(s) 155, 405, 744
CseI GACGC 1 cut(s) 871
CspCI CAANNNNNGTGG 2 cut(s) 625, 660
CviAII CATG 4 cut(s) 118, 171, 575, 625
DdeI CTNAG 4 cut(s) 79, 617, 992, 1062
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
DraI TTTAAA 1 cut(s) 18
EaeI YGGCCR 2 cut(s) 224, 361
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 78
Eco147I AGGCCT 1 cut(s) 43
Eco24I GRGCYC 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 234
Eco32I GATATC 2 cut(s) 564, 844
Eco47I GGWCC 2 cut(s) 155, 744
Eco53kI GAGCTC 1 cut(s) 78
Eco81I CCTNAGG 1 cut(s) 992
EcoICRI GAGCTC 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 407, 487
EcoRV GATATC 2 cut(s) 564, 844
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 80
FaeI CATG 4 cut(s) 121, 174, 578, 628
FatI CATG 4 cut(s) 117, 170, 574, 624
FauI CCCGC 2 cut(s) 155, 858
FauNDI CATATG 1 cut(s) 478
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 3 cut(s) 342, 437, 1074
FokI GGATG 5 cut(s) 432, 509, 512, 813, 817
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 3 cut(s) 342, 437, 1074
FspBI CTAG 6 cut(s) 159, 251, 372, 836, 909, 929
GlaI GCGC 1 cut(s) 1057
GluI GCNGC 3 cut(s) 342, 437, 1074
GsaI CCCAGC 1 cut(s) 466
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 4 cut(s) 43, 226, 363, 407
HapII CCGG 2 cut(s) 227, 924
HgaI GACGC 1 cut(s) 871
HhaI GCGC 1 cut(s) 1058
Hin1II CATG 4 cut(s) 121, 174, 578, 628
Hin6I GCGC 1 cut(s) 1056
HinP1I GCGC 1 cut(s) 1056
HinfI GANTC 3 cut(s) 112, 449, 621
HpaII CCGG 2 cut(s) 227, 924
HphI GGTGA 4 cut(s) 34, 212, 272, 299
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 82, 278, 618, 1012
Hpy188III TCNNGA 4 cut(s) 135, 268, 529, 625
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 6 cut(s) 33, 67, 116, 410, 728, 952
HpyCH4III ACNGT 3 cut(s) 304, 701, 748
HpyCH4V TGCA 5 cut(s) 170, 307, 559, 775, 849
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 433, 521, 929, 1055
HpyF3I CTNAG 4 cut(s) 79, 617, 992, 1062
Hsp92II CATG 4 cut(s) 121, 174, 578, 628
HspAI GCGC 1 cut(s) 1056
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 2 cut(s) 11, 602
Lsp1109I GCAGC 2 cut(s) 423, 1085
LweI GCATC 1 cut(s) 15
MaeI CTAG 6 cut(s) 159, 251, 372, 836, 909, 929
MaeIII GTNAC 1 cut(s) 260
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 6 cut(s) 202, 412, 450, 458, 558, 1009
MhlI GDGCHC 1 cut(s) 80
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 5 cut(s) 309, 535, 674, 751, 875
MluNI TGGCCA 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 722
MnlI CCTC 7 cut(s) 54, 96, 396, 406, 610, 791, 1071
Mox20I TGGCCA 1 cut(s) 363
Mph1103I ATGCAT 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 632
MscI TGGCCA 1 cut(s) 363
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 770
Msp20I TGGCCA 1 cut(s) 363
MspI CCGG 2 cut(s) 227, 924
MspR9I CCNGG 3 cut(s) 409, 489, 925
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 2 cut(s) 409, 489
MvnI CGCG 1 cut(s) 882
MwoI GCNNNNNNNGC 5 cut(s) 11, 433, 521, 929, 1055
NaeI GCCGGC 1 cut(s) 228
NciI CCSGG 1 cut(s) 925
NdeI CATATG 1 cut(s) 478
NdeII GATC 1 cut(s) 316
NgoMIV GCCGGC 1 cut(s) 226
NheI GCTAGC 1 cut(s) 928
NlaIII CATG 4 cut(s) 121, 174, 578, 628
NmuCI GTSAC 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 172
OliI CACNNNNGTG 1 cut(s) 770
PagI TCATGA 1 cut(s) 624
PceI AGGCCT 1 cut(s) 43
PctI GAATGC 1 cut(s) 172
PdiI GCCGGC 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 3 cut(s) 112, 449, 621
PkrI GCNGC 3 cut(s) 343, 438, 1075
PsiI TTATAA 1 cut(s) 822
Psp124BI GAGCTC 1 cut(s) 80
Psp6I CCWGG 2 cut(s) 407, 487
PspFI CCCAGC 1 cut(s) 462
PspGI CCWGG 2 cut(s) 407, 487
PspPI GGNCC 3 cut(s) 155, 405, 744
RseI CAYNNNNRTG 1 cut(s) 770
SacI GAGCTC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 3 cut(s) 342, 437, 1074
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 3 cut(s) 155, 405, 744
ScrFI CCNGG 3 cut(s) 409, 489, 925
SduI GDGCHC 1 cut(s) 80
SfaNI GCATC 1 cut(s) 15
SinI GGWCC 2 cut(s) 155, 744
SmiMI CAYNNNNRTG 1 cut(s) 770
Sse9I AATT 5 cut(s) 309, 535, 674, 751, 875
SseBI AGGCCT 1 cut(s) 43
SsiI CCGC 4 cut(s) 5, 162, 342, 865
SspI AATATT 1 cut(s) 664
SspMI CTAG 6 cut(s) 159, 251, 372, 836, 909, 929
SstI GAGCTC 1 cut(s) 80
StuI AGGCCT 1 cut(s) 43
StyD4I CCNGG 3 cut(s) 407, 487, 923
TaaI ACNGT 3 cut(s) 304, 701, 748
TaqI TCGA 1 cut(s) 452
TasI AATT 5 cut(s) 309, 535, 674, 751, 875
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 3 cut(s) 112, 449, 621
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 3 cut(s) 307, 649, 687
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 2 cut(s) 436, 1073
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 5 cut(s) 338, 495, 591, 639, 641
TspGWI ACGGA 3 cut(s) 412, 806, 1041
TspRI CASTG 3 cut(s) 307, 649, 687
VpaK11BI GGWCC 2 cut(s) 155, 744
XapI RAATTY 3 cut(s) 309, 674, 875
XcmI CCANNNNNNNNNTGG 2 cut(s) 893, 1084
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 6 cut(s) 159, 251, 372, 836, 909, 929
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.