pycom13g02130

B3 domain-containing protein Os01g0723500-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011529
Physical Location & Seq
Reverse (-)
1455563 .. 1455871
309 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02130.1

Sequence Viewer

Length: 309 bp
ATGTATATGGAACGAAAAAGGGGAAGGGATGAGAAATGGCTTGTTCAATTCCAGTCAGAATGCATTGATGAGGGCCGACAAGAAAAAGCCTGGTTCGGATGTGTTTTATTTAAGGTGAAGGCATCGATCCAAAATATGTGTGGAAAAAGAGATGGATTAAATGTTAACGGTAGAACTTGTTTGCTGATGTCATCAGCACATGAAGTAGCTCTATCGTTTAGTCCTCTAATTTCTATTCCGCTTGCTGCTCGTCCAGCCTTTCTCTTTGATTGTACTCATAAGAATTGCCCTAGGGGTTTTTCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

103

Amino Acids

11.65

Weight (kDa)

9.05

Isoelectric Point (pI)

53.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 239
AclWI GGATC 1 cut(s) 121
AfaI GTAC 1 cut(s) 274
AgsI TTSAA 1 cut(s) 47
AjnI CCWGG 1 cut(s) 89
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
AlwI GGATC 1 cut(s) 121
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 1 cut(s) 245
AspA2I CCTAGG 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 1 cut(s) 127
AvrII CCTAGG 1 cut(s) 290
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 91
BfaI CTAG 1 cut(s) 291
BisI GCNGC 1 cut(s) 246
BlnI CCTAGG 1 cut(s) 290
BlsI GCNGC 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 91
BmsI GCATC 1 cut(s) 131
Bsa29I ATCGAT 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 91
BseCI ATCGAT 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 2 cut(s) 34, 104
BseNI ACTGG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 232
BshFI GGCC 1 cut(s) 75
BshVI ATCGAT 1 cut(s) 125
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 1 cut(s) 75
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 1 cut(s) 239
BspANI GGCC 1 cut(s) 75
BspDI ATCGAT 1 cut(s) 125
BspPI GGATC 1 cut(s) 121
BsrI ACTGG 1 cut(s) 52
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 1 cut(s) 170
BstC8I GCNNGC 1 cut(s) 243
BstF5I GGATG 2 cut(s) 34, 104
BstKTI GATC 1 cut(s) 129
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 89
BstV1I GCAGC 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 125
BsuRI GGCC 1 cut(s) 75
BsuTUI ATCGAT 1 cut(s) 125
BtsCI GGATG 2 cut(s) 34, 104
Cac8I GCNNGC 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 73
ClaI ATCGAT 1 cut(s) 125
Csp6I GTAC 1 cut(s) 273
CviAII CATG 1 cut(s) 200
CviJI RGCY 5 cut(s) 40, 75, 89, 209, 257
CviKI_1 RGCY 5 cut(s) 40, 75, 89, 209, 257
CviQI GTAC 1 cut(s) 273
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 89
EcoT14I CCWWGG 1 cut(s) 290
EcoT22I ATGCAT 1 cut(s) 65
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 1 cut(s) 203
FaiI YATR 5 cut(s) 6, 8, 137, 201, 279
FatI CATG 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 2 cut(s) 41, 111
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 1 cut(s) 291
GluI GCNGC 1 cut(s) 246
HaeIII GGCC 1 cut(s) 75
Hin1II CATG 1 cut(s) 203
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HpaI GTTAAC 1 cut(s) 166
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 2 cut(s) 58, 98
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 2 cut(s) 18, 112
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4V TGCA 1 cut(s) 63
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
Hsp92II CATG 1 cut(s) 203
KspAI GTTAAC 1 cut(s) 166
Kzo9I GATC 1 cut(s) 126
LpnPI CCDG 4 cut(s) 65, 76, 103, 267
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 1 cut(s) 131
MaeI CTAG 1 cut(s) 291
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MluCI AATT 3 cut(s) 47, 228, 283
MnlI CCTC 2 cut(s) 64, 234
Mph1103I ATGCAT 1 cut(s) 65
MseI TTAA 4 cut(s) 111, 158, 165, 307
MspR9I CCNGG 1 cut(s) 91
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 1 cut(s) 91
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeII GATC 1 cut(s) 126
NlaIII CATG 1 cut(s) 203
NsiI ATGCAT 1 cut(s) 65
PctI GAATGC 1 cut(s) 65
PkrI GCNGC 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 89
PspPI GGNCC 1 cut(s) 73
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
SaqAI TTAA 4 cut(s) 111, 158, 165, 307
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 73
ScrFI CCNGG 1 cut(s) 91
SetI ASST 2 cut(s) 117, 211
SfaNI GCATC 1 cut(s) 131
Sse9I AATT 3 cut(s) 47, 228, 283
SsiI CCGC 1 cut(s) 239
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 89
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 170
TaqI TCGA 1 cut(s) 125
TasI AATT 3 cut(s) 47, 228, 283
TatI WGTACW 1 cut(s) 272
Tru1I TTAA 4 cut(s) 111, 158, 165, 307
Tru9I TTAA 4 cut(s) 111, 158, 165, 307
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 1 cut(s) 216
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmaJI CCTAGG 1 cut(s) 290
XspI CTAG 1 cut(s) 291
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.