Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004380

Physical Location & Seq

Genomic Coordinates Forward (+)

2520533 .. 2526485

Gene Structure 5953 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1036000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2931 bp

ATGGGGAAGCCTCTTTGCTACGACACAGGGAATGCTGAAGTTTCTCAAGGGCTGATCAATCGGCTTTACAGCGTCTTCTCCGATGGCATACATTTTGCAACGCCGGCTACCTCCTTGAGGACCAATGAACTTGAATTGGTTCGGAGCGTGTTACAAATGTTGCAAGGTTTTTCTAGCTCACTATTTTATTGGGACCAAAATGGAAAATCCTTTCAAGTCAAGAGTGGGATACATGTCAGTCACCTCTCTTACTCGAGTCTTCACGCCCTTGTTCATCAATTTATGCATGCTGCAACATGCTTACAACTGGTGGAAATTATAGTCAACAAAATCGAGAAGTCTGCTGGATTACCACCGCCTACTTTGAGGGCTTTTGCATGTTCTGTTTCTGCGTGGCTTAAGAGGTTGCGCGATATTGCTTTAAAGAAGGAAATGAAGATAAGAGGAGATGGCATTAGTACAACCCCTACGTTATTGGGATTGGCCAATTCTTTGTCTAGTCTCTGCTCAGGTGCTGAGTATCTGTTGCAAATAGTGCATGGTGCCCTCCCACAGGTGTATTTTGAGTCTAATTCTTCTCTTCCCGCTGCTTACTTGGCTGTTCACATCCTTGACCATCTTTACAAGAAGCTGGATGAAGTGTGTCTAGTGCGAGGTGGTGAGGAGGAAGATTACCTGATGCTTCTTTATTTATTTATTGGAAGTATATTGCCGTATATTGAGGGTCTTGATTCTTGGCTTTTTGAAGGAACACTAGATGATCCATATGAGGAGATGTTCTTTTATGCTAATAAAGCAATATCTGTTGACGAGGCTGACTTTTGGGAGAAGAGTTATCTATTAAGGCAAGTACAGTATCAAATGTTAGATGTTGGGACCTCTGCCTCAATGACAAGTTTTATGAAAGGAAAGGAATGTGGCAATAAAGACCTTCAATCATGTCCATTGTTTATTAAGGACATAGCTAAGTCTATTGTTTCTGCTGGAAAATCGTTACAGCTGATCCGGCATATTCCCATGACATCCTCAGTTGTATATCGAAACGGAAATGACTCTGAGGTTGATGGTTTTGGAAGTTTTAATAAAGGTGTTTACCATGGGCAAAGCATTGCAGGTTTGACTTTGTCAGAAGTTTTCTGTGTGTCGTTAGCAGGCCTTATAGGCCATGGTGACCATATCTTTCAACATATATCCACTAAACAAAAAGTGGAGAGTGATGATAGTGTGATTGTGCCGGTAAAATGTTCGGAGAAAATTTGGTGTAAGTTCTTGGTTGATACACTGGCAGAGAAAAGAGTAACTGAACCAGAGTCTGCATGTGATAATGGAAAAAGATTTACAGATGCAAATGAAGAGAAAATGTTTGCAGGTGTAGTAAATGGGTTTCCTCATTCAAGATCCTTCTGTCAAGAAAATCCAGTTCTAACGGTCTGCCAAAAGATCCTTTCTAAGAATGGGGATGCATGGAAATCCTTGAACTTATCCAGAAATTTATGTCTGCCCCCTTTAAACGATGAAGCTTTACGAAAGGCTATTTTCGGTGTGGAGAGTGGATCAACTTCTTTGGCTGAAGGAACAAATTATACTTTTGGTTTCCGCTTTGGTGAATCAGAATATCTTCGTTCACAAGACGACTCGCATATGTTACAATCGTTGTTTCCTTTTCCTACTCTTCTACCATCTGTTCAGGATGAGCTCTGTATGTCAGAGCTCTTACCTTTCCAAAAAAATAGCACCCTTCCATCAAGAGTTCTTGCCTGGATTCAACATTTTGAACCAAGAAGTACTCCACTTCCGGTGGTGATAGTGCAGGAATGTCTTACTGTCTACATGCAGAAGCAGGTGGATTGTATTGGCAGGCATATTTTGTCAAAACTGATGAATGACTGGAAATTGATGGATGAACTGGCAGTCTTGCGCGCGATTTACTTGCTAGGGTCAGGTGATCTGCTACAGCACTTCTTGACTGTAATATTCAATAAGCTGGATAAAGGAGAAACATGGGATGATGATTTTGAGTTAAACACGATATTGCAGGAATCTATAAGAAACTCTGCTGATGGAGTGCTACTAAGTTTTCCTGATTCATTGATTGTATCCCTCACCAAAAATCACGACTTAAATGGAAATGAGCAACCTAAGATGGCTTCCCTTCCCTCAACTCCTCGTAAAAGTCGTGCTCAGAGCTTTGGAATGGATGGACTTGATCTGCTAAACTTCACATATAAGGTCTCTTGGCCACTTGAACTTATTGCAAACGTGGAGGCAATCAAGAAATATAACCAGGTCATGGGGTTCTTGTTGAAGGTTAAGCGAGCAAAATTCGTACTTGATAAAGCTCGAAGGTGGATGTGGAAGGGTAGAGGCAGTGCCGCAAACAACCACAAGCGTCACTGGTTAGTGGAGCAAAAACTTCTCCATTTTGTCGATGCTTTTCACCAGTATGTGATGGACAGAGTATATCACAATGCATGGCGTGAACTATGTGAAGGTATGGCAGCTGCTCGATCTCTGGACGAAGTCATAGAAGTACATGAATTGTACTTGTTGACAATTCAGCGGCAGTGCTTTGTAGTTCCAGATAAGCTGTGGGCTCTCATAGCAAGCAGGATAAACAACATCTTGGGATTAGCATTGGACTTCTATTCTATACAACTGACGCTTAGCGGCGGAACAGTTTCTGCAATTAAGGCCAAATGCGAAATGGAAGTTGATCGTATTGAGAAGCAGTTTGATGATTGCATTGCTTTCCTGCTCAGAGTGCTTTCGTTCAAGCTCAACGTCGGGCATTTTCCTCATTTAGCAGATTTGGTTACTAGGATCAATTACAATTACTTTTACATGTCGGATGCTGGAAACCTAAGGACTCTCCCGAGCTCCGAAAATGTTGCTTCGAGGCTGGGGAAGGCTTTTCTAGGCAGAACAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009653 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0099120 GO:0099568 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spc97_Spc98

Protein Analysis

977

Amino Acids

109.8

Weight (kDa)

6.24

Isoelectric Point (pI)

45.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCP_N_terminal	PF17681	47 - 343	3.5e-22	Gamma tubulin complex component N-terminal
GCP_C_terminal	PF04130	632 - 947	2.2e-65	Gamma tubulin complex component C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004380)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20570 AT1G20570 AT1G20570 AT1G20570 AT1G80260 AT1G80260 AT1G80260
fragaria_vesca	FvH4_2g40750 FvH4_2g40750 FvH4_2g40750
malus_domestica	MD15G1036000.v1.1
prunus_persica	Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1
pyrus_communis	pycom15g03350
rosa_chinensis	RchiOBHm_Chr6g0299841
rosa_laevigata	RLG00000011384
rosa_multiflora	Rmu_ssc0000048.1_g000001
rosa_roxburghii	Rroxscaffold_7G00168330
rosa_rugosa	Rorug06G0292600
rosa_samantha	Rh6AG403800 Rh6BG412100 Rh6CG418200 Rh6DG405000
rosa_wichuraiana	Rw6G035330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1358, 1831
Acc36I	ACCTGC	3 cut(s)	1103, 1358, 1831
AccB1I	GGYRCC	1 cut(s)	542
AccB7I	CCANNNNNTGG	1 cut(s)	2290
AccI	GTMKAC	1 cut(s)	1827
AccII	CGCG	3 cut(s)	411, 1920, 1922
AciI	CCGC	7 cut(s)	356, 585, 1597, 2373, 2560, 2667, 2670
AclWI	GGATC	6 cut(s)	755, 997, 1392, 1435, 1561, 2828
AcoI	YGGCCR	2 cut(s)	483, 2237
AcsI	RAATTY	3 cut(s)	1254, 1489, 2321
AcuI	CTGAAG	2 cut(s)	57, 1590
AfaI	GTAC	6 cut(s)	460, 852, 1786, 2328, 2532, 2543
AfiI	CCNNNNNNNGG	3 cut(s)	117, 553, 2290
AflII	CTTAAG	1 cut(s)	398
AflIII	ACRYGT	2 cut(s)	232, 2841
AjnI	CCWGG	2 cut(s)	1757, 2283
Alw21I	GWGCWC	4 cut(s)	1698, 1713, 2182, 2879
Alw26I	GTCTC	2 cut(s)	506, 2236
AlwI	GGATC	6 cut(s)	755, 997, 1392, 1435, 1561, 2828
AlwNI	CAGNNNCTG	3 cut(s)	515, 1313, 2681
Ama87I	CYCGRG	2 cut(s)	253, 2872
AoxI	GGCC	5 cut(s)	483, 1153, 1162, 2237, 2691
ApeKI	GCWGC	4 cut(s)	290, 587, 2498, 2501
ApoI	RAATTY	3 cut(s)	1254, 1489, 2321
Asp700I	GAANNNNTTC	3 cut(s)	138, 1617, 2677
AspLEI	GCGC	3 cut(s)	411, 1920, 1922
AspS9I	GGNCC	3 cut(s)	120, 193, 876
AsuHPI	GGTGA	8 cut(s)	233, 671, 1181, 1616, 1813, 1955, 2095, 2429
AvaI	CYCGRG	2 cut(s)	253, 2872
AvaII	GGWCC	3 cut(s)	120, 193, 876
AxyI	CCTNAGG	1 cut(s)	2861
BaeGI	GKGCMC	1 cut(s)	547
BalI	TGGCCA	2 cut(s)	485, 2239
BanI	GGYRCC	1 cut(s)	542
BanII	GRGCYC	4 cut(s)	1698, 1713, 2596, 2879
BbsI	GAAGAC	2 cut(s)	67, 251
Bbv12I	GWGCWC	4 cut(s)	1698, 1713, 2182, 2879
BbvI	GCAGC	4 cut(s)	277, 574, 2488, 2510
BceAI	ACGGC	1 cut(s)	697
BcgI	CGANNNNNNTGC	6 cut(s)	323, 357, 1912, 1946, 2870, 2904
BciT130I	CCWGG	2 cut(s)	1759, 2285
BciVI	GTATCC	2 cut(s)	222, 2107
BclI	TGATCA	1 cut(s)	54
BcoDI	GTCTC	2 cut(s)	506, 2236
BfaI	CTAG	7 cut(s)	174, 498, 647, 755, 1934, 2817, 2915
BfmI	CTRYAG	1 cut(s)	1952
BfrI	CTTAAG	1 cut(s)	398
BfuAI	ACCTGC	3 cut(s)	1103, 1358, 1831
BfuI	GTATCC	2 cut(s)	222, 2107
BglI	GCCNNNNNGGC	1 cut(s)	1161
BisI	GCNGC	7 cut(s)	291, 588, 2373, 2499, 2502, 2561, 2668
BlpI	GCTNAGC	1 cut(s)	2663
BlsI	GCNGC	7 cut(s)	292, 589, 2374, 2500, 2503, 2562, 2669
BmcAI	AGTACT	1 cut(s)	1786
Bme1390I	CCNGG	2 cut(s)	1759, 2285
Bme18I	GGWCC	3 cut(s)	120, 193, 876
BmeT110I	CYCGRG	2 cut(s)	253, 2872
BmgT120I	GGNCC	3 cut(s)	120, 193, 876
BmiI	GGNNCC	3 cut(s)	194, 544, 877
BmrFI	CCNGG	2 cut(s)	1759, 2285
BmsI	GCATC	5 cut(s)	669, 1333, 1450, 2419, 2839
BpiI	GAAGAC	2 cut(s)	67, 251
Bpu10I	CCTNAGC	1 cut(s)	508
Bpu1102I	GCTNAGC	1 cut(s)	2663
BpuEI	CTTGAG	2 cut(s)	30, 136
BsaI	GGTCTC	1 cut(s)	2236
BsaJI	CCNNGG	2 cut(s)	1096, 1165
BsaWI	WCCGGW	1 cut(s)	1795
Bsc4I	CCNNNNNNNGG	3 cut(s)	117, 553, 2290
Bse118I	RCCGGY	2 cut(s)	103, 1234
Bse1I	ACTGG	7 cut(s)	312, 1287, 1418, 1892, 1911, 2399, 2440
Bse21I	CCTNAGG	1 cut(s)	2861
Bse3DI	GCAATG	2 cut(s)	1107, 2742
BseBI	CCWGG	2 cut(s)	1759, 2285
BseDI	CCNNGG	2 cut(s)	1096, 1165
BseLI	CCNNNNNNNGG	3 cut(s)	117, 553, 2290
BseMI	GCAATG	2 cut(s)	1107, 2742
BseMII	CTCAG	6 cut(s)	507, 522, 1041, 1047, 2195, 2770
BseNI	ACTGG	7 cut(s)	312, 1287, 1418, 1892, 1911, 2399, 2440
BsePI	GCGCGC	1 cut(s)	1918
BseRI	GAGGAG	4 cut(s)	459, 677, 785, 2154
BseSI	GKGCMC	1 cut(s)	547
BseXI	GCAGC	4 cut(s)	277, 574, 2488, 2510
BseYI	CCCAGC	1 cut(s)	2899
BsgI	GTGCAG	1 cut(s)	1829
Bsh1236I	CGCG	3 cut(s)	411, 1920, 1922
BshFI	GGCC	5 cut(s)	485, 1155, 1164, 2239, 2693
BshNI	GGYRCC	1 cut(s)	542
BsiHKAI	GWGCWC	4 cut(s)	1698, 1713, 2182, 2879
BsiHKCI	CYCGRG	2 cut(s)	253, 2872
BsiSI	CCGG	4 cut(s)	104, 1006, 1235, 1796
BslFI	GGGAC	2 cut(s)	206, 889
BslI	CCNNNNNNNGG	3 cut(s)	117, 553, 2290
BsmAI	GTCTC	2 cut(s)	506, 2236
BsmFI	GGGAC	2 cut(s)	206, 889
BsmI	GAATGC	1 cut(s)	37
BsnI	GGCC	5 cut(s)	485, 1155, 1164, 2239, 2693
Bso31I	GGTCTC	1 cut(s)	2236
BsoBI	CYCGRG	2 cut(s)	253, 2872
Bsp1286I	GDGCHC	6 cut(s)	547, 1698, 1713, 2182, 2596, 2879
Bsp1720I	GCTNAGC	1 cut(s)	2663
Bsp19I	CCATGG	2 cut(s)	1096, 1165
BspACI	CCGC	7 cut(s)	356, 585, 1597, 2373, 2560, 2667, 2670
BspANI	GGCC	5 cut(s)	485, 1155, 1164, 2239, 2693
BspCNI	CTCAG	6 cut(s)	508, 521, 1040, 1048, 2194, 2769
BspFNI	CGCG	3 cut(s)	411, 1920, 1922
BspLI	GGNNCC	3 cut(s)	194, 544, 877
BspMI	ACCTGC	3 cut(s)	1103, 1358, 1831
BspPI	GGATC	6 cut(s)	755, 997, 1392, 1435, 1561, 2828
BspT107I	GGYRCC	1 cut(s)	542
BspTI	CTTAAG	1 cut(s)	398
BspTNI	GGTCTC	1 cut(s)	2236
BsrDI	GCAATG	2 cut(s)	1107, 2742
BsrFI	RCCGGY	2 cut(s)	103, 1234
BsrI	ACTGG	7 cut(s)	312, 1287, 1418, 1892, 1911, 2399, 2440
BssAI	RCCGGY	2 cut(s)	103, 1234
BssECI	CCNNGG	2 cut(s)	1096, 1165
BssHII	GCGCGC	1 cut(s)	1918
BssT1I	CCWWGG	2 cut(s)	1096, 1165
Bst2UI	CCWGG	2 cut(s)	1759, 2285
Bst4CI	ACNGT	5 cut(s)	855, 1429, 1825, 1969, 2677
Bst6I	CTCTTC	4 cut(s)	585, 824, 1347, 1677
BstAFI	CTTAAG	1 cut(s)	398
BstAPI	GCANNNNNTGC	1 cut(s)	535
BstC8I	GCNNGC	7 cut(s)	105, 288, 1153, 1859, 1920, 2316, 2605
BstDSI	CCRYGG	2 cut(s)	1096, 1165
BstEII	GGTNACC	1 cut(s)	1169
BstENI	CCTNNNNNAGG	2 cut(s)	115, 551
BstFNI	CGCG	3 cut(s)	411, 1920, 1922
BstHHI	GCGC	3 cut(s)	411, 1920, 1922
BstMAI	GTCTC	2 cut(s)	506, 2236
BstMWI	GCNNNNNNNGC	9 cut(s)	104, 535, 596, 794, 1006, 1161, 2600, 2690, 2761
BstNI	CCWGG	2 cut(s)	1759, 2285
BstNSI	RCATGY	7 cut(s)	236, 290, 300, 381, 1320, 1834, 2845
BstPI	GGTNACC	1 cut(s)	1169
BstSCI	CCNGG	2 cut(s)	1757, 2283
BstSFI	CTRYAG	1 cut(s)	1952
BstSLI	GKGCMC	1 cut(s)	547
BstUI	CGCG	3 cut(s)	411, 1920, 1922
BstV1I	GCAGC	4 cut(s)	277, 574, 2488, 2510
BstV2I	GAAGAC	2 cut(s)	67, 251
BstX2I	RGATCY	2 cut(s)	1397, 1440
BstYI	RGATCY	2 cut(s)	1397, 1440
Bsu36I	CCTNAGG	1 cut(s)	2861
BsuI	GTATCC	2 cut(s)	222, 2107
BsuRI	GGCC	5 cut(s)	485, 1155, 1164, 2239, 2693
BtgI	CCRYGG	2 cut(s)	1096, 1165
BtsI	GCAGTG	2 cut(s)	2374, 2570
BtsIMutI	CAGTG	4 cut(s)	1280, 2374, 2392, 2570
BveI	ACCTGC	3 cut(s)	1103, 1358, 1831
Cac8I	GCNNGC	7 cut(s)	105, 288, 1153, 1859, 1920, 2316, 2605
CaiI	CAGNNNCTG	3 cut(s)	515, 1313, 2681
CfoI	GCGC	3 cut(s)	411, 1920, 1922
Cfr10I	RCCGGY	2 cut(s)	103, 1234
Cfr13I	GGNCC	3 cut(s)	120, 193, 876
CseI	GACGC	3 cut(s)	61, 2378, 2668
CsiI	ACCWGGT	1 cut(s)	2283
Csp6I	GTAC	6 cut(s)	459, 851, 1785, 2327, 2531, 2542
CviQI	GTAC	6 cut(s)	459, 851, 1785, 2327, 2531, 2542
DraI	TTTAAA	2 cut(s)	423, 1509
EaeI	YGGCCR	2 cut(s)	483, 2237
Eam1104I	CTCTTC	4 cut(s)	585, 824, 1347, 1677
EarI	CTCTTC	4 cut(s)	585, 824, 1347, 1677
EciI	GGCGGA	1 cut(s)	2685
Ecl136II	GAGCTC	3 cut(s)	1696, 1711, 2877
Eco130I	CCWWGG	2 cut(s)	1096, 1165
Eco147I	AGGCCT	1 cut(s)	1155
Eco24I	GRGCYC	4 cut(s)	1698, 1713, 2596, 2879
Eco31I	GGTCTC	1 cut(s)	2236
Eco47I	GGWCC	3 cut(s)	120, 193, 876
Eco53kI	GAGCTC	3 cut(s)	1696, 1711, 2877
Eco57I	CTGAAG	2 cut(s)	57, 1590
Eco81I	CCTNAGG	1 cut(s)	2861
Eco88I	CYCGRG	2 cut(s)	253, 2872
Eco91I	GGTNACC	1 cut(s)	1169
EcoICRI	GAGCTC	3 cut(s)	1696, 1711, 2877
EcoNI	CCTNNNNNAGG	2 cut(s)	115, 551
EcoO109I	RGGNCCY	1 cut(s)	876
EcoO65I	GGTNACC	1 cut(s)	1169
EcoRII	CCWGG	2 cut(s)	1757, 2283
EcoT14I	CCWWGG	2 cut(s)	1096, 1165
EcoT22I	ATGCAT	3 cut(s)	288, 1465, 2473
EcoT38I	GRGCYC	4 cut(s)	1698, 1713, 2596, 2879
ErhI	CCWWGG	2 cut(s)	1096, 1165
FaqI	GGGAC	2 cut(s)	206, 889
FauI	CCCGC	1 cut(s)	592
FauNDI	CATATG	2 cut(s)	766, 1641
FbaI	TGATCA	1 cut(s)	54
FblI	GTMKAC	1 cut(s)	1827
Fnu4HI	GCNGC	7 cut(s)	291, 588, 2373, 2499, 2502, 2561, 2668
FriOI	GRGCYC	4 cut(s)	1698, 1713, 2596, 2879
Fsp4HI	GCNGC	7 cut(s)	291, 588, 2373, 2499, 2502, 2561, 2668
FspBI	CTAG	7 cut(s)	174, 498, 647, 755, 1934, 2817, 2915
GlaI	GCGC	3 cut(s)	410, 1919, 1921
GluI	GCNGC	7 cut(s)	291, 588, 2373, 2499, 2502, 2561, 2668
GsaI	CCCAGC	1 cut(s)	2903
HaeIII	GGCC	5 cut(s)	485, 1155, 1164, 2239, 2693
HapII	CCGG	4 cut(s)	104, 1006, 1235, 1796
HgaI	GACGC	3 cut(s)	61, 2378, 2668
HhaI	GCGC	3 cut(s)	411, 1920, 1922
Hin6I	GCGC	3 cut(s)	409, 1918, 1920
HinP1I	GCGC	3 cut(s)	409, 1918, 1920
HincII	GTYRAC	3 cut(s)	325, 808, 2550
HindII	GTYRAC	3 cut(s)	325, 808, 2550
HindIII	AAGCTT	1 cut(s)	1518
HpaII	CCGG	4 cut(s)	104, 1006, 1235, 1796
HphI	GGTGA	8 cut(s)	233, 671, 1181, 1616, 1813, 1955, 2095, 2429
Hpy166II	GTNNAC	8 cut(s)	325, 604, 808, 1093, 1625, 1828, 2480, 2550
Hpy8I	GTNNAC	8 cut(s)	325, 604, 808, 1093, 1625, 1828, 2480, 2550
Hpy99I	CGWCG	1 cut(s)	2786
HpyCH4III	ACNGT	5 cut(s)	855, 1429, 1825, 1969, 2677
HpyCH4IV	ACGT	3 cut(s)	470, 2259, 2781
HpyF10VI	GCNNNNNNNGC	9 cut(s)	104, 535, 596, 794, 1006, 1161, 2600, 2690, 2761
HpySE526I	ACGT	3 cut(s)	470, 2259, 2781
HspAI	GCGC	3 cut(s)	409, 1918, 1920
KroI	GCCGGC	1 cut(s)	103
KroNI	GCCGGC	1 cut(s)	105
Ksp22I	TGATCA	1 cut(s)	54
LmnI	GCTCC	3 cut(s)	144, 2404, 2882
Lsp1109I	GCAGC	4 cut(s)	277, 574, 2488, 2510
LweI	GCATC	5 cut(s)	669, 1333, 1450, 2419, 2839
MabI	ACCWGGT	1 cut(s)	2283
MaeI	CTAG	7 cut(s)	174, 498, 647, 755, 1934, 2817, 2915
MaeII	ACGT	3 cut(s)	470, 2259, 2781
MaeIII	GTNAC	8 cut(s)	150, 239, 993, 1169, 1297, 1644, 2390, 2812
MflI	RGATCY	2 cut(s)	1397, 1440
MhlI	GDGCHC	6 cut(s)	547, 1698, 1713, 2182, 2596, 2879
MlsI	TGGCCA	2 cut(s)	485, 2239
MluNI	TGGCCA	2 cut(s)	485, 2239
MlyI	GAGTC	6 cut(s)	265, 575, 1046, 1319, 1628, 2860
MmeI	TCCRAC	1 cut(s)	2826
Mox20I	TGGCCA	2 cut(s)	485, 2239
Mph1103I	ATGCAT	3 cut(s)	288, 1465, 2473
MroNI	GCCGGC	1 cut(s)	103
MroXI	GAANNNNTTC	3 cut(s)	138, 1617, 2677
MscI	TGGCCA	2 cut(s)	485, 2239
MslI	CAYNNNNRTG	1 cut(s)	2442
Msp20I	TGGCCA	2 cut(s)	485, 2239
MspA1I	CMGCKG	4 cut(s)	587, 1000, 2501, 2560
MspCI	CTTAAG	1 cut(s)	398
MspI	CCGG	4 cut(s)	104, 1006, 1235, 1796
MspR9I	CCNGG	2 cut(s)	1759, 2285
Mva1269I	GAATGC	1 cut(s)	37
MvaI	CCWGG	2 cut(s)	1759, 2285
MvnI	CGCG	3 cut(s)	411, 1920, 1922
MwoI	GCNNNNNNNGC	9 cut(s)	104, 535, 596, 794, 1006, 1161, 2600, 2690, 2761
NaeI	GCCGGC	1 cut(s)	105
NcoI	CCATGG	2 cut(s)	1096, 1165
NdeI	CATATG	2 cut(s)	766, 1641
NgoMIV	GCCGGC	1 cut(s)	103
NlaIV	GGNNCC	3 cut(s)	194, 544, 877
NmuCI	GTSAC	3 cut(s)	239, 1169, 2390
NsiI	ATGCAT	3 cut(s)	288, 1465, 2473
NspI	RCATGY	7 cut(s)	236, 290, 300, 381, 1320, 1834, 2845
PaeI	GCATGC	1 cut(s)	290
PaeR7I	CTCGAG	1 cut(s)	253
PaqCI	CACCTGC	2 cut(s)	1358, 1831
PauI	GCGCGC	1 cut(s)	1918
PceI	AGGCCT	1 cut(s)	1155
PciI	ACATGT	2 cut(s)	232, 2841
PcsI	WCGNNNNNNNCGW	1 cut(s)	2173
PctI	GAATGC	1 cut(s)	37
PdiI	GCCGGC	1 cut(s)	105
PdmI	GAANNNNTTC	3 cut(s)	138, 1617, 2677
PfeI	GAWTC	5 cut(s)	731, 1607, 1762, 2039, 2084
PflFI	GACNNNGTC	2 cut(s)	1123, 2519
PflMI	CCANNNNNTGG	1 cut(s)	2290
PkrI	GCNGC	7 cut(s)	292, 589, 2374, 2500, 2503, 2562, 2669
PleI	GAGTC	6 cut(s)	264, 574, 1046, 1318, 1628, 2860
PpsI	GAGTC	6 cut(s)	264, 574, 1046, 1318, 1628, 2860
PpuMI	RGGWCCY	1 cut(s)	876
PscI	ACATGT	2 cut(s)	232, 2841
Psp124BI	GAGCTC	3 cut(s)	1698, 1713, 2879
Psp5II	RGGWCCY	1 cut(s)	876
Psp6I	CCWGG	2 cut(s)	1757, 2283
PspEI	GGTNACC	1 cut(s)	1169
PspFI	CCCAGC	1 cut(s)	2899
PspGI	CCWGG	2 cut(s)	1757, 2283
PspN4I	GGNNCC	3 cut(s)	194, 544, 877
PspPI	GGNCC	3 cut(s)	120, 193, 876
PspPPI	RGGWCCY	1 cut(s)	876
PspXI	VCTCGAGB	1 cut(s)	253
PsrI	GAACNNNNNNTAC	2 cut(s)	1567, 1599
PstNI	CAGNNNCTG	3 cut(s)	515, 1313, 2681
PsuI	RGATCY	2 cut(s)	1397, 1440
PsyI	GACNNNGTC	2 cut(s)	1123, 2519
PteI	GCGCGC	1 cut(s)	1918
PvuII	CAGCTG	2 cut(s)	1000, 2501
RsaI	GTAC	6 cut(s)	460, 852, 1786, 2328, 2532, 2543
RsaNI	GTAC	6 cut(s)	459, 851, 1785, 2327, 2531, 2542
RseI	CAYNNNNRTG	1 cut(s)	2442
SacI	GAGCTC	3 cut(s)	1698, 1713, 2879
SatI	GCNGC	7 cut(s)	291, 588, 2373, 2499, 2502, 2561, 2668
Sau96I	GGNCC	3 cut(s)	120, 193, 876
ScaI	AGTACT	1 cut(s)	1786
SchI	GAGTC	6 cut(s)	265, 575, 1046, 1319, 1628, 2860
ScrFI	CCNGG	2 cut(s)	1759, 2285
SduI	GDGCHC	6 cut(s)	547, 1698, 1713, 2182, 2596, 2879
SexAI	ACCWGGT	1 cut(s)	2283
SfaNI	GCATC	5 cut(s)	669, 1333, 1450, 2419, 2839
SfcI	CTRYAG	1 cut(s)	1952
SfiI	GGCCNNNNNGGCC	1 cut(s)	1161
Sfr274I	CTCGAG	1 cut(s)	253
SinI	GGWCC	3 cut(s)	120, 193, 876
SlaI	CTCGAG	1 cut(s)	253
SmiMI	CAYNNNNRTG	1 cut(s)	2442
SmlI	CTYRAG	4 cut(s)	45, 115, 253, 398
SmoI	CTYRAG	4 cut(s)	45, 115, 253, 398
SphI	GCATGC	1 cut(s)	290
SseBI	AGGCCT	1 cut(s)	1155
SsiI	CCGC	7 cut(s)	356, 585, 1597, 2373, 2560, 2667, 2670
SspI	AATATT	1 cut(s)	1974
SspMI	CTAG	7 cut(s)	174, 498, 647, 755, 1934, 2817, 2915
SstI	GAGCTC	3 cut(s)	1698, 1713, 2879
StuI	AGGCCT	1 cut(s)	1155
StyD4I	CCNGG	2 cut(s)	1757, 2283
StyI	CCWWGG	2 cut(s)	1096, 1165
TaaI	ACNGT	5 cut(s)	855, 1429, 1825, 1969, 2677
TaiI	ACGT	3 cut(s)	473, 2262, 2784
TaqI	TCGA	7 cut(s)	254, 333, 1039, 2341, 2427, 2506, 2894
TatI	WGTACW	5 cut(s)	458, 850, 1784, 2530, 2541
TauI	GCSGC	3 cut(s)	2375, 2563, 2670
TfiI	GAWTC	5 cut(s)	731, 1607, 1762, 2039, 2084
TscAI	CASTG	4 cut(s)	1287, 2374, 2399, 2570
TseFI	GTSAC	3 cut(s)	239, 1169, 2390
TseI	GCWGC	4 cut(s)	290, 587, 2498, 2501
Tsp45I	GTSAC	3 cut(s)	239, 1169, 2390
TspGWI	ACGGA	1 cut(s)	1059
TspRI	CASTG	4 cut(s)	1287, 2374, 2399, 2570
Tth111I	GACNNNGTC	2 cut(s)	1123, 2519
Van91I	CCANNNNNTGG	1 cut(s)	2290
Vha464I	CTTAAG	1 cut(s)	398
VpaK11BI	GGWCC	3 cut(s)	120, 193, 876
XagI	CCTNNNNNAGG	2 cut(s)	115, 551
XapI	RAATTY	3 cut(s)	1254, 1489, 2321
XceI	RCATGY	7 cut(s)	236, 290, 300, 381, 1320, 1834, 2845
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2586, 2701
XhoI	CTCGAG	1 cut(s)	253
XmiI	GTMKAC	1 cut(s)	1827
XmnI	GAANNNNTTC	3 cut(s)	138, 1617, 2677
XspI	CTAG	7 cut(s)	174, 498, 647, 755, 1934, 2817, 2915
ZrmI	AGTACT	1 cut(s)	1786
Zsp2I	ATGCAT	3 cut(s)	288, 1465, 2473

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.