Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0004380

Physical Location & Seq

Genomic Coordinates Reverse (-)

59876738 .. 59881908

Gene Structure 5171 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G035330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2943 bp

ATGGGGAAGCCTCTCTGCTACGATGCAGGGAATGCAGAAGTTTCTCAAGCTCTAATCAACAAGATCTACGGCGTTTTCTCCCATGGCCTCCATTTCGCAACACCAGTCTCCTCCCTGAGAACCAATGAACTCGATTTGGTTCGGAGTGTGTTACAAATGTTACAAGGATATTCCAGTTCACTATTTTATTGGGATCATAATGCAAACTCTTTTCAAGCCAAAACTGGGATATATGTTAATCACCTCTCCCATACAAGTCTTGACGCCATTGTCAGTCAGTTTATGTATGCTGCAACTTGCTTGCAACTCGTAGAAATTGTGGTCAACAAAGTCGAGAAATCGGCGAGATTACCTCCTCCTCCTACTTTGAGGGCTTTTGTGTGTTCTGTTTCTGCATGGCTTAAGAGGTGGCGTGATGTTGCTCTAAAGGAGGAAGTGAGGGTAAGCAAAGATGACAATGGAACGACCCCTACATTGTTGGGATTGGCCAGCTCCTTATCCAGTCTATGCTCAGGTGCTGAGTCTCTGTTGCAAATAGTGCATTGTGCCATTCCCCAAGTGTATTTTGAGTCCGATCCTTCTCTTTCTGCTGCTGAGTTGACTCTTCATGTCCTTGACTCTCTTTACAAGAAACTAGATGAAGTATGCCTTGTCCGAGGTGGTGAGGATGAAGATTATCAGATGTTACTTCATTTATTTATTGGAAGTATATTGCCATATATTGAGGGTCTTGATTCTTGGCTTTTTGAAGGAACACTTGATGATCCCTATGAGGAGATGTTCTTCTATCCCAATAGAGCAGTTTCAGTTGATAAGGCTGACTTCTGGGAGAAGAGCTATCTATTGAGGCAAGTGCGGGGTCAAATCTTAGACGTTGGGTGTGCTGCTCCATCTCTTGCCAGTGACCGTGCATCTGTAATAAATGACAAAAGGGGAGCGGGTCAGAGGGAATCCATCTCATGTCCGTTGTTTATTAAGGACATAGCTAAGTCGATCGTTTCTGCTGGAAAATCATTACAGCTGATCCGGCATATTCCTATGACTTCTTTAGGTGAAGGAAATTACTGTGCAATTGATGGTTTTGGCAGTTCGAATAAAGGTGTTCACCATGGGGAGAGCATTGCAGGTTTAACTTTGTCAGAAGTTTTTTGCGTGTCATTAGCAGGTCTTGTAGGACATGGTGATCATATCTTTCAGGCTATTGCAAGTAAACAAAAACTGGAAAGTGATGATGGTGTGATTGAGTCATTAAGAAGCTCAGAAAAACCTTGGTGTAAGTTCTTGGTTGATACGCTGTTGGAAAAAAGATTGATTGAAACCAACTCTCCACATGCGGATGGAAAAGCATTCCCTGGTGCGAAAGAAGAGAGTATGTTTTCAGATTTGGTAGACAAATGTCCCCTTTCAAGATCCTTCTGTCAAGAAAATCCAGTTACAACAGTCTGCCAAAAAATCCTTTTTAAGAATGGGGATGCATGGAAAACCTTGAACTTATCTAGAAATTTTAGCCTGCCCCCTTTAAATGATGAAGTTCTACGAGCAGCTATATTTGGCGGGGATAGTGGATCAACTTCTTCGGCCAAAGGAACCAACTATACATTTGGTTTTCGGTTTGGTGAATCCGAACATATTCGTTCACAAGACGACTCAAAAATGTTACAAATGTTGTTTCCTTTTCCCACTCTTCTTCCTTCTTTTGAGGATGATCTCCGCATGTCAGAGCTCTTACCTTTCCAGAAAAATAGCACCCTTCCATCTAGAGTTCTTACTTGGATTCAGTATTTTGAACCGAGAAGTACTCCACTTCCCGTGGTTATAGTGCAGGAATGTCTTACTGCCTACATTCAGAAGCAGGTGGATTGTATTGGCAGGCATGTATTGTCGAAATTGATGAATGATTGGAAGTTGATGGATGAACTGGCAGTGTTGCGTGCTATTTACTTGTTAGGGTCAGGTGATCTACTACAGCACTTCTTGACCATAATTTTCAATAAGCTGGATAAAGGAGAAACATGGGATGATGATTTTGAGTTAAACACTATATTGCAGGAATCTATAAGAAACTCTGCTGATGGTGTGCTACTAAGTGTTCCTGATTCATTGATTGTATCCCTCACCAAAAATCAAGATTTGAATAGCAATGAGCAGCCTAGTATGGCTTCCCTTCCCTCAACTCCTCATAAAAGTCGTGCTAACAGCTTTGGAATGGATGGACTTGATCTACTGCAGTTTACATACAAGGTCTCTTGGCCACTTGAACTTATTGCAAATGCAGAAGCGATCAAGAAATATAACCAAGTCATGGGATTCTTGCTGAAGGTCAAGCGAGCAAAGTTTGTACTGGATAAAGCTCGAAGATGGATGTGGAAGGGTAGAGGCAGGGCAGCGAAGAGCTTTAAACATCACTGGTTAGTGGAGCAGAAACTTCTCCATTTTGTGGATGCGTTTCACCAGTATGTGATGGACAGAGTGTATCACAATGCATGGCGTGAACTTTGTGAAGGTATGGCAGCCGCTCGTTCTCTGGATGAAGTCATTGAAGTTCATGAGTCGTACTTGTTGTCAATTCAGCGACAGTGCTTTGTAGTTCCAGATAAGCTGTGGGCTCTCATTGCAACCAGGATAAACAACATTTTGGGATTAGCATTGGACTTTTACTCTATACAGCTGACACTTAGTGGTGGAGCAGTTTCTGCAATCAAGGCCAAATGTGAAATGGAAGTTGAACGTATTGAGAAACAGTTCGATGATTGCATCGCTTTTCTGCTCAGAGTACTCTCCTTCAAACTCAATGTAGGACACTTCCCTCATTTAGCAGATTTGGTTACTAGGATCAACTACAACCACTTTTACATGTCAGATACTGGAAACCTACGGACTCTCCCAAGCTCTGAAAATGTTGCTTCTAGACTGGGAAAAGCTTTTTTAGGTAGAACAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009653 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0099120 GO:0099568 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spc97_Spc98

Protein Analysis

980

Amino Acids

109.72

Weight (kDa)

6.06

Isoelectric Point (pI)

41.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCP5-Mod21_C	PF20685	43 - 95	3.9e-06	Gamma-Tubulin ring complex non-core subunit mod21, C-terminal
GCP_N_terminal	PF17681	47 - 345	3.6e-21	Gamma tubulin complex component N-terminal
GCP_C_terminal	PF04130	636 - 951	4e-66	Gamma tubulin complex component C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004380)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20570 AT1G20570 AT1G20570 AT1G20570 AT1G80260 AT1G80260 AT1G80260
fragaria_vesca	FvH4_2g40750 FvH4_2g40750 FvH4_2g40750
malus_domestica	MD15G1036000.v1.1
prunus_persica	Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1 Prupe.1G389900_v2.0.a1
pyrus_communis	pycom15g03350
rosa_chinensis	RchiOBHm_Chr6g0299841
rosa_laevigata	RLG00000011384
rosa_multiflora	Rmu_ssc0000048.1_g000001
rosa_roxburghii	Rroxscaffold_7G00168330
rosa_rugosa	Rorug06G0292600
rosa_samantha	Rh6AG403800 Rh6BG412100 Rh6CG418200 Rh6DG405000
rosa_wichuraiana	Rw6G035330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1843
AasI	GACNNNNNNGTC	1 cut(s)	269
Acc36I	ACCTGC	3 cut(s)	1115, 1154, 1843
AccB7I	CCANNNNNTGG	2 cut(s)	2302, 2437
AccBSI	CCGCTC	2 cut(s)	938, 2516
AccI	GTMKAC	1 cut(s)	1389
AciI	CCGC	6 cut(s)	856, 938, 1334, 1554, 1711, 2514
AclWI	GGATC	7 cut(s)	201, 569, 758, 1018, 1404, 1573, 2840
AcoI	YGGCCR	3 cut(s)	486, 1578, 2249
AcsI	RAATTY	1 cut(s)	1501
AcuI	CTGAAG	1 cut(s)	2336
AcyI	GRCGYC	1 cut(s)	264
AdeI	CACNNNGTG	1 cut(s)	2678
AfaI	GTAC	4 cut(s)	1798, 2340, 2555, 2775
AfiI	CCNNNNNNNGG	3 cut(s)	225, 2302, 2437
AflII	CTTAAG	1 cut(s)	401
AflIII	ACRYGT	1 cut(s)	2853
AjnI	CCWGG	2 cut(s)	1351, 2618
Alw21I	GWGCWC	1 cut(s)	1725
Alw26I	GTCTC	3 cut(s)	112, 528, 2248
AlwI	GGATC	7 cut(s)	201, 569, 758, 1018, 1404, 1573, 2840
AlwNI	CAGNNNCTG	3 cut(s)	518, 2693, 2864
AoxI	GGCC	5 cut(s)	85, 486, 1578, 2249, 2703
ApeKI	GCWGC	7 cut(s)	290, 590, 884, 1541, 2146, 2384, 2510
ApoI	RAATTY	1 cut(s)	1501
Asp700I	GAANNNNTTC	2 cut(s)	1629, 2741
AsuHPI	GGTGA	9 cut(s)	233, 674, 1064, 1097, 1193, 1628, 1967, 2107, 2441
AsuII	TTCGAA	1 cut(s)	1091
BalI	TGGCCA	2 cut(s)	488, 2251
BanII	GRGCYC	2 cut(s)	1725, 2608
Bbv12I	GWGCWC	1 cut(s)	1725
BbvI	GCAGC	7 cut(s)	277, 577, 871, 1553, 2158, 2396, 2522
BceAI	ACGGC	1 cut(s)	85
BciT130I	CCWGG	2 cut(s)	1353, 2620
BciVI	GTATCC	1 cut(s)	2119
BclI	TGATCA	1 cut(s)	1183
BcoDI	GTCTC	3 cut(s)	112, 528, 2248
BfaI	CTAG	6 cut(s)	635, 1497, 1758, 2151, 2829, 2907
BfmI	CTRYAG	2 cut(s)	1964, 2225
BfrI	CTTAAG	1 cut(s)	401
BfuAI	ACCTGC	3 cut(s)	1115, 1154, 1843
BfuI	GTATCC	1 cut(s)	2119
BglII	AGATCT	1 cut(s)	63
BisI	GCNGC	8 cut(s)	291, 591, 885, 1542, 2147, 2385, 2511, 2514
BlsI	GCNGC	8 cut(s)	292, 592, 886, 1543, 2148, 2386, 2512, 2515
BmcAI	AGTACT	2 cut(s)	1798, 2775
Bme1390I	CCNGG	2 cut(s)	1353, 2620
BmiI	GGNNCC	1 cut(s)	1588
BmrFI	CCNGG	2 cut(s)	1353, 2620
BmrI	ACTGGG	2 cut(s)	234, 2921
BmsI	GCATC	5 cut(s)	13, 920, 1462, 2431, 2763
BmuI	ACTGGG	2 cut(s)	234, 2921
BoxI	GACNNNNGTC	1 cut(s)	1395
BplI	GAGNNNNNCTC	4 cut(s)	337, 369, 1783, 1815
Bpu10I	CCTNAGC	1 cut(s)	511
Bpu14I	TTCGAA	1 cut(s)	1091
BpuEI	CTTGAG	1 cut(s)	30
BsaHI	GRCGYC	1 cut(s)	264
BsaI	GGTCTC	1 cut(s)	2248
BsaJI	CCNNGG	6 cut(s)	82, 655, 1108, 1268, 1351, 1809
BsaXI	ACNNNNNCTCC	4 cut(s)	1309, 1339, 2865, 2895
Bsc4I	CCNNNNNNNGG	3 cut(s)	225, 2302, 2437
Bse3DI	GCAATG	3 cut(s)	1119, 2146, 2610
BseBI	CCWGG	2 cut(s)	1353, 2620
BseDI	CCNNGG	6 cut(s)	82, 655, 1108, 1268, 1351, 1809
BseLI	CCNNNNNNNGG	3 cut(s)	225, 2302, 2437
BseMI	GCAATG	3 cut(s)	1119, 2146, 2610
BseMII	CTCAG	6 cut(s)	107, 510, 525, 585, 1272, 2782
BseRI	GAGGAG	5 cut(s)	100, 345, 348, 788, 2166
BseXI	GCAGC	7 cut(s)	277, 577, 871, 1553, 2158, 2396, 2522
BsgI	GTGCAG	1 cut(s)	1841
Bsh1285I	CGRYCG	1 cut(s)	996
BshFI	GGCC	5 cut(s)	87, 488, 1580, 2251, 2705
BsiEI	CGRYCG	1 cut(s)	996
BsiHKAI	GWGCWC	1 cut(s)	1725
BsiSI	CCGG	1 cut(s)	1027
BslFI	GGGAC	1 cut(s)	1383
BslI	CCNNNNNNNGG	3 cut(s)	225, 2302, 2437
BsmAI	GTCTC	3 cut(s)	112, 528, 2248
BsmFI	GGGAC	1 cut(s)	1383
BsmI	GAATGC	2 cut(s)	37, 1346
BsnI	GGCC	5 cut(s)	87, 488, 1580, 2251, 2705
Bso31I	GGTCTC	1 cut(s)	2248
Bsp119I	TTCGAA	1 cut(s)	1091
Bsp1286I	GDGCHC	2 cut(s)	1725, 2608
Bsp19I	CCATGG	2 cut(s)	82, 1108
BspACI	CCGC	6 cut(s)	856, 938, 1334, 1554, 1711, 2514
BspANI	GGCC	5 cut(s)	87, 488, 1580, 2251, 2705
BspCNI	CTCAG	6 cut(s)	108, 511, 524, 586, 1271, 2781
BspHI	TCATGA	1 cut(s)	2545
BspLI	GGNNCC	1 cut(s)	1588
BspMAI	CTGCAG	1 cut(s)	2229
BspMI	ACCTGC	3 cut(s)	1115, 1154, 1843
BspPI	GGATC	7 cut(s)	201, 569, 758, 1018, 1404, 1573, 2840
BspQI	GCTCTTC	2 cut(s)	827, 2384
BspT104I	TTCGAA	1 cut(s)	1091
BspTI	CTTAAG	1 cut(s)	401
BspTNI	GGTCTC	1 cut(s)	2248
BsrBI	CCGCTC	2 cut(s)	938, 2516
BsrDI	GCAATG	3 cut(s)	1119, 2146, 2610
BssECI	CCNNGG	6 cut(s)	82, 655, 1108, 1268, 1351, 1809
BssNI	GRCGYC	1 cut(s)	264
BssT1I	CCWWGG	3 cut(s)	82, 1108, 1268
Bst2UI	CCWGG	2 cut(s)	1353, 2620
Bst4CI	ACNGT	5 cut(s)	908, 1067, 1441, 2577, 2742
Bst6I	CTCTTC	5 cut(s)	609, 827, 1359, 1689, 2384
BstACI	GRCGYC	1 cut(s)	264
BstAFI	CTTAAG	1 cut(s)	401
BstAPI	GCANNNNNTGC	3 cut(s)	32, 538, 2693
BstBI	TTCGAA	1 cut(s)	1091
BstC8I	GCNNGC	6 cut(s)	302, 490, 1511, 1871, 1932, 2328
BstDSI	CCRYGG	3 cut(s)	82, 1108, 1809
BstMAI	GTCTC	3 cut(s)	112, 528, 2248
BstMCI	CGRYCG	1 cut(s)	996
BstMWI	GCNNNNNNNGC	6 cut(s)	32, 538, 1027, 2612, 2693, 2702
BstNI	CCWGG	2 cut(s)	1353, 2620
BstNSI	RCATGY	4 cut(s)	1334, 1717, 1877, 2857
BstPAI	GACNNNNGTC	1 cut(s)	1395
BstSCI	CCNGG	2 cut(s)	1351, 2618
BstSFI	CTRYAG	2 cut(s)	1964, 2225
BstV1I	GCAGC	7 cut(s)	277, 577, 871, 1553, 2158, 2396, 2522
BstX2I	RGATCY	2 cut(s)	63, 1409
BstYI	RGATCY	2 cut(s)	63, 1409
BsuI	GTATCC	1 cut(s)	2119
BsuRI	GGCC	5 cut(s)	87, 488, 1580, 2251, 2705
BtgI	CCRYGG	3 cut(s)	82, 1108, 1809
BtgZI	GCGATG	1 cut(s)	2740
BtsI	GCAGTG	1 cut(s)	1929
BtsIMutI	CAGTG	4 cut(s)	907, 1929, 2404, 2582
BveI	ACCTGC	3 cut(s)	1115, 1154, 1843
Cac8I	GCNNGC	6 cut(s)	302, 490, 1511, 1871, 1932, 2328
CaiI	CAGNNNCTG	3 cut(s)	518, 2693, 2864
CciI	TCATGA	1 cut(s)	2545
CseI	GACGC	1 cut(s)	272
Csp6I	GTAC	4 cut(s)	1797, 2339, 2554, 2774
CviQI	GTAC	4 cut(s)	1797, 2339, 2554, 2774
DraI	TTTAAA	2 cut(s)	1521, 2398
DraIII	CACNNNGTG	1 cut(s)	2678
DrdI	GACNNNNNNGTC	1 cut(s)	269
DseDI	GACNNNNNNGTC	1 cut(s)	269
EaeI	YGGCCR	3 cut(s)	486, 1578, 2249
Eam1104I	CTCTTC	5 cut(s)	609, 827, 1359, 1689, 2384
EarI	CTCTTC	5 cut(s)	609, 827, 1359, 1689, 2384
Ecl136II	GAGCTC	1 cut(s)	1723
Eco130I	CCWWGG	3 cut(s)	82, 1108, 1268
Eco24I	GRGCYC	2 cut(s)	1725, 2608
Eco31I	GGTCTC	1 cut(s)	2248
Eco53kI	GAGCTC	1 cut(s)	1723
Eco57I	CTGAAG	1 cut(s)	2336
EcoICRI	GAGCTC	1 cut(s)	1723
EcoRII	CCWGG	2 cut(s)	1351, 2618
EcoT14I	CCWWGG	3 cut(s)	82, 1108, 1268
EcoT22I	ATGCAT	2 cut(s)	1477, 2485
EcoT38I	GRGCYC	2 cut(s)	1725, 2608
ErhI	CCWWGG	3 cut(s)	82, 1108, 1268
FalI	AAGNNNNNCTT	6 cut(s)	633, 665, 741, 773, 806, 838
FaqI	GGGAC	1 cut(s)	1383
FauI	CCCGC	3 cut(s)	849, 931, 1547
FbaI	TGATCA	1 cut(s)	1183
FblI	GTMKAC	1 cut(s)	1389
Fnu4HI	GCNGC	8 cut(s)	291, 591, 885, 1542, 2147, 2385, 2511, 2514
FriOI	GRGCYC	2 cut(s)	1725, 2608
Fsp4HI	GCNGC	8 cut(s)	291, 591, 885, 1542, 2147, 2385, 2511, 2514
FspBI	CTAG	6 cut(s)	635, 1497, 1758, 2151, 2829, 2907
GluI	GCNGC	8 cut(s)	291, 591, 885, 1542, 2147, 2385, 2511, 2514
HaeIII	GGCC	5 cut(s)	87, 488, 1580, 2251, 2705
HapII	CCGG	1 cut(s)	1027
HgaI	GACGC	1 cut(s)	272
Hin1I	GRCGYC	1 cut(s)	264
HincII	GTYRAC	2 cut(s)	325, 600
HindII	GTYRAC	2 cut(s)	325, 600
HindIII	AAGCTT	1 cut(s)	2919
HpaII	CCGG	1 cut(s)	1027
HphI	GGTGA	9 cut(s)	233, 674, 1064, 1097, 1193, 1628, 1967, 2107, 2441
Hpy166II	GTNNAC	9 cut(s)	179, 325, 600, 1105, 1211, 1390, 1637, 2232, 2492
Hpy8I	GTNNAC	9 cut(s)	179, 325, 600, 1105, 1211, 1390, 1637, 2232, 2492
HpyCH4III	ACNGT	5 cut(s)	908, 1067, 1441, 2577, 2742
HpyCH4IV	ACGT	2 cut(s)	873, 2728
HpyF10VI	GCNNNNNNNGC	6 cut(s)	32, 538, 1027, 2612, 2693, 2702
HpySE526I	ACGT	2 cut(s)	873, 2728
Hsp92I	GRCGYC	1 cut(s)	264
Ksp22I	TGATCA	1 cut(s)	1183
LguI	GCTCTTC	2 cut(s)	827, 2384
LmnI	GCTCC	5 cut(s)	497, 892, 935, 2416, 2684
Lsp1109I	GCAGC	7 cut(s)	277, 577, 871, 1553, 2158, 2396, 2522
LweI	GCATC	5 cut(s)	13, 920, 1462, 2431, 2763
MaeI	CTAG	6 cut(s)	635, 1497, 1758, 2151, 2829, 2907
MaeII	ACGT	2 cut(s)	873, 2728
MaeIII	GTNAC	7 cut(s)	150, 159, 684, 902, 1432, 1656, 2824
MbiI	CCGCTC	2 cut(s)	938, 2516
MfeI	CAATTG	1 cut(s)	1071
MflI	RGATCY	2 cut(s)	63, 1409
MhlI	GDGCHC	2 cut(s)	1725, 2608
MlsI	TGGCCA	2 cut(s)	488, 2251
MluCI	AATT	7 cut(s)	315, 1060, 1071, 1501, 1886, 1983, 2565
MluNI	TGGCCA	2 cut(s)	488, 2251
MlyI	GAGTC	8 cut(s)	530, 578, 595, 611, 1253, 1640, 2558, 2872
MmeI	TCCRAC	1 cut(s)	1278
Mox20I	TGGCCA	2 cut(s)	488, 2251
Mph1103I	ATGCAT	2 cut(s)	1477, 2485
MroXI	GAANNNNTTC	2 cut(s)	1629, 2741
MscI	TGGCCA	2 cut(s)	488, 2251
MseI	TTAA	9 cut(s)	237, 402, 975, 1130, 1250, 1461, 1520, 2033, 2397
MslI	CAYNNNNRTG	2 cut(s)	1335, 2454
Msp20I	TGGCCA	2 cut(s)	488, 2251
MspA1I	CMGCKG	2 cut(s)	1021, 2668
MspCI	CTTAAG	1 cut(s)	401
MspI	CCGG	1 cut(s)	1027
MspR9I	CCNGG	2 cut(s)	1353, 2620
MunI	CAATTG	1 cut(s)	1071
Mva1269I	GAATGC	2 cut(s)	37, 1346
MvaI	CCWGG	2 cut(s)	1353, 2620
MwoI	GCNNNNNNNGC	6 cut(s)	32, 538, 1027, 2612, 2693, 2702
NcoI	CCATGG	2 cut(s)	82, 1108
NlaIV	GGNNCC	1 cut(s)	1588
NmuCI	GTSAC	1 cut(s)	902
NsiI	ATGCAT	2 cut(s)	1477, 2485
NspI	RCATGY	4 cut(s)	1334, 1717, 1877, 2857
NspV	TTCGAA	1 cut(s)	1091
PagI	TCATGA	1 cut(s)	2545
PaqCI	CACCTGC	1 cut(s)	1843
PciI	ACATGT	1 cut(s)	2853
PciSI	GCTCTTC	2 cut(s)	827, 2384
PctI	GAATGC	2 cut(s)	37, 1346
PdmI	GAANNNNTTC	2 cut(s)	1629, 2741
PfeI	GAWTC	7 cut(s)	734, 950, 1619, 1774, 2051, 2096, 2307
PflMI	CCANNNNNTGG	2 cut(s)	2302, 2437
PkrI	GCNGC	8 cut(s)	292, 592, 886, 1543, 2148, 2386, 2512, 2515
Ple19I	CGATCG	1 cut(s)	996
PleI	GAGTC	8 cut(s)	529, 577, 595, 611, 1252, 1640, 2557, 2872
PpsI	GAGTC	8 cut(s)	529, 577, 595, 611, 1252, 1640, 2557, 2872
PscI	ACATGT	1 cut(s)	2853
PshAI	GACNNNNGTC	1 cut(s)	1395
Psp124BI	GAGCTC	1 cut(s)	1725
Psp6I	CCWGG	2 cut(s)	1351, 2618
PspGI	CCWGG	2 cut(s)	1351, 2618
PspN4I	GGNNCC	1 cut(s)	1588
PsrI	GAACNNNNNNTAC	4 cut(s)	454, 486, 1579, 1611
PstI	CTGCAG	1 cut(s)	2229
PstNI	CAGNNNCTG	3 cut(s)	518, 2693, 2864
PsuI	RGATCY	2 cut(s)	63, 1409
PvuI	CGATCG	1 cut(s)	996
PvuII	CAGCTG	2 cut(s)	1021, 2668
RsaI	GTAC	4 cut(s)	1798, 2340, 2555, 2775
RsaNI	GTAC	4 cut(s)	1797, 2339, 2554, 2774
RseI	CAYNNNNRTG	2 cut(s)	1335, 2454
SacI	GAGCTC	1 cut(s)	1725
SapI	GCTCTTC	2 cut(s)	827, 2384
SaqAI	TTAA	9 cut(s)	237, 402, 975, 1130, 1250, 1461, 1520, 2033, 2397
SatI	GCNGC	8 cut(s)	291, 591, 885, 1542, 2147, 2385, 2511, 2514
ScaI	AGTACT	2 cut(s)	1798, 2775
SchI	GAGTC	8 cut(s)	530, 578, 595, 611, 1253, 1640, 2558, 2872
ScrFI	CCNGG	2 cut(s)	1353, 2620
SduI	GDGCHC	2 cut(s)	1725, 2608
SfaNI	GCATC	5 cut(s)	13, 920, 1462, 2431, 2763
SfcI	CTRYAG	2 cut(s)	1964, 2225
SfuI	TTCGAA	1 cut(s)	1091
SmiMI	CAYNNNNRTG	2 cut(s)	1335, 2454
SmlI	CTYRAG	2 cut(s)	45, 401
SmoI	CTYRAG	2 cut(s)	45, 401
Sse9I	AATT	7 cut(s)	315, 1060, 1071, 1501, 1886, 1983, 2565
SsiI	CCGC	6 cut(s)	856, 938, 1334, 1554, 1711, 2514
SspMI	CTAG	6 cut(s)	635, 1497, 1758, 2151, 2829, 2907
SstI	GAGCTC	1 cut(s)	1725
StyD4I	CCNGG	2 cut(s)	1351, 2618
StyI	CCWWGG	3 cut(s)	82, 1108, 1268
TaaI	ACNGT	5 cut(s)	908, 1067, 1441, 2577, 2742
TaiI	ACGT	2 cut(s)	876, 2731
TaqI	TCGA	7 cut(s)	132, 333, 992, 1091, 1883, 2353, 2745
TasI	AATT	7 cut(s)	315, 1060, 1071, 1501, 1886, 1983, 2565
TatI	WGTACW	3 cut(s)	1796, 2338, 2773
TauI	GCSGC	1 cut(s)	2516
TfiI	GAWTC	7 cut(s)	734, 950, 1619, 1774, 2051, 2096, 2307
Tru1I	TTAA	9 cut(s)	237, 402, 975, 1130, 1250, 1461, 1520, 2033, 2397
Tru9I	TTAA	9 cut(s)	237, 402, 975, 1130, 1250, 1461, 1520, 2033, 2397
TscAI	CASTG	4 cut(s)	907, 1929, 2411, 2582
TseFI	GTSAC	1 cut(s)	902
TseI	GCWGC	7 cut(s)	290, 590, 884, 1541, 2146, 2384, 2510
Tsp45I	GTSAC	1 cut(s)	902
TspGWI	ACGGA	2 cut(s)	954, 2890
TspRI	CASTG	4 cut(s)	907, 1929, 2411, 2582
Van91I	CCANNNNNTGG	2 cut(s)	2302, 2437
Vha464I	CTTAAG	1 cut(s)	401
XapI	RAATTY	1 cut(s)	1501
XbaI	TCTAGA	3 cut(s)	1496, 1757, 2906
XceI	RCATGY	4 cut(s)	1334, 1717, 1877, 2857
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2598, 2713
XmiI	GTMKAC	1 cut(s)	1389
XmnI	GAANNNNTTC	2 cut(s)	1629, 2741
XspI	CTAG	6 cut(s)	635, 1497, 1758, 2151, 2829, 2907
ZrmI	AGTACT	2 cut(s)	1798, 2775
Zsp2I	ATGCAT	2 cut(s)	1477, 2485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.