Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012580

Physical Location & Seq

Genomic Coordinates Reverse (-)

4846587 .. 4850040

Gene Structure 3454 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1070900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1683 bp

ATGAAGAGAAAAAGAGACGAAAATGGTGATCCTTTATCGCGGGACCTTTCTTGCCGAGAAAGTGACAGCTTTTTCTTCAACGGAGATTGTGGAGAAGACAAACCAATCCTCAATCTCAACAACTATTGTGATGGCTTTGCACTGGAAGAAGACCACTTGCAGAACTTAATTCTCAGAGAGATCGAATTTGGGTTCGAGAAAGACGAAGATTTGGTGATTCCCATTTGGGTTTTGAACGCTCGAGTGGAATCCATCACATGGGTTCTCCAAAGAAGAAGGGCTCTTGGATTTCATCCCAGAACAGCATATCTGTGCATGACATACTTTGATCGGTTCATTTCCCTGAGACAAATCCCTGTTAATAATAAAGAAGCTGTGGTTAGATTACTTTCAGTGGCGTGTCTATCTCTGGCTGCAAAGATGGACGAGCAGAACATTCCATCACTGTCACAGTACGAAGTCGAAAATCCTCACCTTTTTGAAGTAAAACTGATTCAGAGAATGGAGCTTACGGTGTTGGGAGCACTGGAATGGAAGATGAATATGATTACACCCTTCTCGTTTATCGATTACTTCGTCTCCAAGTTGTGCCAAGAATCTCTATCTGATGTAAAATCCAAGATTGCAGGCCTCCTCCTTTCCATAATCACAGAGATTAATTCAATGCATCAACGACCGTCCGCTGTAGCAGCCGCGGCCACTTTGATGGCATTGGATCAGAAACTAACAAAAGAAGCATTGGAATTAAAGATAAACTCTGTATCAGAACTGAAATTTTTGGAAATTAGCTTTCTTGCCAAGAAAGAAACAATGGACGGTGGCAATGACGATGATCCTTTATCGCCGGACCTTTCTTGCCAAGAAAATCCTGAATTTTTGTACGATGAGGTGCCAGTTGAAAACCCAGATGACGGCATCAACAACTTAATCGACATAGAGATCGGATTCGGGTTCCAGAGAGATGAAGCTTTGATCATTCCCAACTGGGTTTTGGAAGTTCGATCGGAATCCATCACATGGGCTCTCAACATTGTAGAAGAAAGACCTGAAATTAGATTGCTTTCGGTGGCCTGCCTTTCGTTGGCGGCATGGACGGAGAGGATGATGCTCCCACCGCTGTCACAACACGAAGACGGAGATTACTTCTTTGCAACGGGATCTATTGTACAAAAGGCGCACGGGGTGGTGACAGCATTAGGATGGGACATGGATATAATCACACCCTTCGCGTTTCTCGATTACTTGGTCTCCAAGTTATGGCAAGAATTTCCACCAGATGTGAAATTTCAGATTGCAGCACACCTCCGTTCTGTAATGACAGAGATCAATTTAATGCATCATCGGCCGTCCGCCGTAGCAGCTGCCGCCACCTTAATGGCATTGGATCAGAATTTAACTAATGAATCATTGAATGTCAGGATATCGGTTCCTGATTTGAATTTTTTGGAAACTGTGAGTTTTTTTGGTTCGTTAATTAATCTCATTAGTTGGTTAATCAAATGGATTAAAGACTGGATTTTGCTTAATGGGTTGATTAATCTTGGACTTGTTGGCCCTTGTGTTGCAGGACGCTGTGTTTTTCTTGTACCATCTAATGCAGGAGCGGTTAGGTGGTTGAAAGTAAATAACCCAAAAGGTGCAGTAAATGTTGTAGAAAATTCTTCAGTTACAGCAGAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0022402 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0071704 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyclin_C Cyclin_N

Protein Analysis

561

Amino Acids

63.18

Weight (kDa)

4.8

Isoelectric Point (pI)

41.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyclin_N	PF00134	79 - 181	3e-20	Cyclin, N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012580)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g33190
malus_domestica	MD08G1085300.v1.1 MD08G1085700.v1.1 MD15G1070900.v1.1
prunus_persica	Prupe.1G423600_v2.0.a1
pyrus_communis	pycom08g07030 pycom15g06670
rosa_chinensis	RchiOBHm_Chr6g0309481
rosa_laevigata	RLG00000010562
rosa_multiflora	Rmu_sc0001212.1_g000003 Rmu_sc0007283.1_g000002 Rmu_sc0022500.1_g000003
rosa_roxburghii	Rroxscaffold_7G00159370
rosa_rugosa	Rorug06G0372300
rosa_samantha	Rh6AG485500 Rh6BG494900 Rh6CG499900 Rh6DG485900
rosa_wichuraiana	Rw6G042290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	889
AccB7I	CCANNNNNTGG	3 cut(s)	258, 1017, 1257
AccBSI	CCGCTC	1 cut(s)	1604
AccII	CGCG	3 cut(s)	40, 695, 1229
AciI	CCGC	9 cut(s)	40, 681, 693, 695, 1085, 1115, 1350, 1365, 1604
AclWI	GGATC	5 cut(s)	23, 723, 827, 1165, 1392
AcoI	YGGCCR	2 cut(s)	696, 1343
AcsI	RAATTY	8 cut(s)	185, 773, 872, 1265, 1283, 1390, 1438, 1657
AcuI	CTGAAG	1 cut(s)	1647
AdeI	CACNNNGTG	1 cut(s)	1183
AfaI	GTAC	4 cut(s)	455, 881, 1167, 1587
AfiI	CCNNNNNNNGG	5 cut(s)	258, 911, 1017, 1081, 1257
AgsI	TTSAA	8 cut(s)	79, 235, 482, 663, 899, 1411, 1438, 1618
AluBI	AGCT	6 cut(s)	69, 374, 508, 789, 968, 1361
AluI	AGCT	6 cut(s)	69, 374, 508, 789, 968, 1361
Alw21I	GWGCWC	1 cut(s)	526
Alw26I	GTCTC	4 cut(s)	9, 340, 583, 1252
AlwI	GGATC	5 cut(s)	23, 723, 827, 1165, 1392
Ama87I	CYCGRG	1 cut(s)	240
AoxI	GGCC	5 cut(s)	628, 696, 1068, 1343, 1552
ApeKI	GCWGC	5 cut(s)	413, 689, 1295, 1358, 1361
ApoI	RAATTY	8 cut(s)	185, 773, 872, 1265, 1283, 1390, 1438, 1657
AseI	ATTAAT	3 cut(s)	657, 1476, 1536
AspLEI	GCGC	1 cut(s)	1177
AspS9I	GGNCC	3 cut(s)	43, 847, 1553
AsuHPI	GGTGA	4 cut(s)	38, 226, 464, 1198
AvaI	CYCGRG	1 cut(s)	240
AvaII	GGWCC	2 cut(s)	43, 847
BanI	GGYRCC	1 cut(s)	889
BanII	GRGCYC	2 cut(s)	283, 1024
BbsI	GAAGAC	3 cut(s)	102, 156, 1137
Bbv12I	GWGCWC	1 cut(s)	526
BbvI	GCAGC	5 cut(s)	400, 701, 1307, 1348, 1370
BccI	CCATC	8 cut(s)	125, 260, 415, 448, 700, 1019, 1194, 1597
BceAI	ACGGC	3 cut(s)	928, 1330, 1337
BclI	TGATCA	1 cut(s)	972
BcoDI	GTCTC	4 cut(s)	9, 340, 583, 1252
BfmI	CTRYAG	1 cut(s)	684
BisI	GCNGC	9 cut(s)	414, 690, 693, 696, 1086, 1296, 1359, 1362, 1365
BlsI	GCNGC	9 cut(s)	415, 691, 694, 697, 1087, 1297, 1360, 1363, 1366
Bme18I	GGWCC	2 cut(s)	43, 847
BmeT110I	CYCGRG	1 cut(s)	240
BmgT120I	GGNCC	3 cut(s)	43, 847, 1553
BmiI	GGNNCC	4 cut(s)	44, 891, 953, 1428
BmrI	ACTGGG	1 cut(s)	994
BmsI	GCATC	4 cut(s)	676, 924, 1095, 1345
BmuI	ACTGGG	1 cut(s)	994
BpiI	GAAGAC	3 cut(s)	102, 156, 1137
Bsa29I	ATCGAT	1 cut(s)	567
BsaBI	GATNNNNATC	1 cut(s)	1006
BsaI	GGTCTC	1 cut(s)	1252
BsaJI	CCNNGG	1 cut(s)	693
BsaXI	ACNNNNNCTCC	4 cut(s)	563, 593, 1232, 1262
Bsc4I	CCNNNNNNNGG	5 cut(s)	258, 911, 1017, 1081, 1257
Bse1I	ACTGG	5 cut(s)	147, 531, 893, 989, 1517
Bse3DI	GCAATG	1 cut(s)	829
Bse8I	GATNNNNATC	1 cut(s)	1006
BseCI	ATCGAT	1 cut(s)	567
BseDI	CCNNGG	1 cut(s)	693
BseGI	GGATG	3 cut(s)	292, 1107, 1205
BseJI	GATNNNNATC	1 cut(s)	1006
BseLI	CCNNNNNNNGG	5 cut(s)	258, 911, 1017, 1081, 1257
BseMI	GCAATG	1 cut(s)	829
BseMII	CTCAG	2 cut(s)	187, 335
BseNI	ACTGG	5 cut(s)	147, 531, 893, 989, 1517
BseRI	GAGGAG	1 cut(s)	623
BseX3I	CGGCCG	1 cut(s)	1343
BseXI	GCAGC	5 cut(s)	400, 701, 1307, 1348, 1370
BsgI	GTGCAG	1 cut(s)	1659
Bsh1236I	CGCG	3 cut(s)	40, 695, 1229
Bsh1285I	CGRYCG	3 cut(s)	677, 1004, 1346
BshFI	GGCC	5 cut(s)	630, 698, 1070, 1345, 1554
BshNI	GGYRCC	1 cut(s)	889
BshVI	ATCGAT	1 cut(s)	567
BsiEI	CGRYCG	3 cut(s)	677, 1004, 1346
BsiHKAI	GWGCWC	1 cut(s)	526
BsiHKCI	CYCGRG	1 cut(s)	240
BsiSI	CCGG	1 cut(s)	845
BslFI	GGGAC	2 cut(s)	56, 1217
BslI	CCNNNNNNNGG	5 cut(s)	258, 911, 1017, 1081, 1257
BsmAI	GTCTC	4 cut(s)	9, 340, 583, 1252
BsmBI	CGTCTC	2 cut(s)	9, 583
BsmFI	GGGAC	2 cut(s)	56, 1217
BsnI	GGCC	5 cut(s)	630, 698, 1070, 1345, 1554
Bso31I	GGTCTC	1 cut(s)	1252
BsoBI	CYCGRG	1 cut(s)	240
Bsp1286I	GDGCHC	3 cut(s)	283, 526, 1024
Bsp1407I	TGTACA	1 cut(s)	1165
BspACI	CCGC	9 cut(s)	40, 681, 693, 695, 1085, 1115, 1350, 1365, 1604
BspANI	GGCC	5 cut(s)	630, 698, 1070, 1345, 1554
BspCNI	CTCAG	2 cut(s)	186, 336
BspDI	ATCGAT	1 cut(s)	567
BspFNI	CGCG	3 cut(s)	40, 695, 1229
BspLI	GGNNCC	4 cut(s)	44, 891, 953, 1428
BspPI	GGATC	5 cut(s)	23, 723, 827, 1165, 1392
BspT107I	GGYRCC	1 cut(s)	889
BspTNI	GGTCTC	1 cut(s)	1252
BsrBI	CCGCTC	1 cut(s)	1604
BsrDI	GCAATG	1 cut(s)	829
BsrGI	TGTACA	1 cut(s)	1165
BsrI	ACTGG	5 cut(s)	147, 531, 893, 989, 1517
BssECI	CCNNGG	1 cut(s)	693
Bst4CI	ACNGT	6 cut(s)	447, 453, 514, 678, 818, 1453
BstAUI	TGTACA	1 cut(s)	1165
BstC8I	GCNNGC	2 cut(s)	628, 1072
BstDEI	CTNAG	2 cut(s)	173, 344
BstDSI	CCRYGG	1 cut(s)	693
BstF5I	GGATG	3 cut(s)	292, 1107, 1205
BstFNI	CGCG	3 cut(s)	40, 695, 1229
BstHHI	GCGC	1 cut(s)	1177
BstMAI	GTCTC	4 cut(s)	9, 340, 583, 1252
BstMCI	CGRYCG	3 cut(s)	677, 1004, 1346
BstMWI	GCNNNNNNNGC	6 cut(s)	689, 695, 1114, 1342, 1358, 1364
BstSFI	CTRYAG	1 cut(s)	684
BstUI	CGCG	3 cut(s)	40, 695, 1229
BstV1I	GCAGC	5 cut(s)	400, 701, 1307, 1348, 1370
BstV2I	GAAGAC	3 cut(s)	102, 156, 1137
BstX2I	RGATCY	1 cut(s)	1157
BstXI	CCANNNNNNTGG	2 cut(s)	706, 1375
BstYI	RGATCY	1 cut(s)	1157
BstZI	CGGCCG	1 cut(s)	1343
Bsu15I	ATCGAT	1 cut(s)	567
BsuRI	GGCC	5 cut(s)	630, 698, 1070, 1345, 1554
BsuTUI	ATCGAT	1 cut(s)	567
BtgI	CCRYGG	1 cut(s)	693
BtsCI	GGATG	3 cut(s)	292, 1107, 1205
BtsIMutI	CAGTG	4 cut(s)	140, 399, 443, 524
Cac8I	GCNNGC	2 cut(s)	628, 1072
CfoI	GCGC	1 cut(s)	1177
Cfr13I	GGNCC	3 cut(s)	43, 847, 1553
Cfr42I	CCGCGG	1 cut(s)	696
ClaI	ATCGAT	1 cut(s)	567
CseI	GACGC	1 cut(s)	1578
Csp6I	GTAC	4 cut(s)	454, 880, 1166, 1586
CviAII	CATG	5 cut(s)	258, 316, 1017, 1089, 1207
CviQI	GTAC	4 cut(s)	454, 880, 1166, 1586
DdeI	CTNAG	2 cut(s)	173, 344
DraIII	CACNNNGTG	1 cut(s)	1183
EaeI	YGGCCR	2 cut(s)	696, 1343
EagI	CGGCCG	1 cut(s)	1343
EciI	GGCGGA	1 cut(s)	1339
EclXI	CGGCCG	1 cut(s)	1343
Eco147I	AGGCCT	1 cut(s)	630
Eco24I	GRGCYC	2 cut(s)	283, 1024
Eco31I	GGTCTC	1 cut(s)	1252
Eco32I	GATATC	1 cut(s)	1422
Eco47I	GGWCC	2 cut(s)	43, 847
Eco52I	CGGCCG	1 cut(s)	1343
Eco57I	CTGAAG	1 cut(s)	1647
Eco88I	CYCGRG	1 cut(s)	240
EcoO109I	RGGNCCY	1 cut(s)	43
EcoRV	GATATC	1 cut(s)	1422
EcoT22I	ATGCAT	2 cut(s)	669, 1338
EcoT38I	GRGCYC	2 cut(s)	283, 1024
Esp3I	CGTCTC	2 cut(s)	9, 583
FaeI	CATG	5 cut(s)	261, 319, 1020, 1092, 1210
FaqI	GGGAC	2 cut(s)	56, 1217
FatI	CATG	5 cut(s)	257, 315, 1016, 1088, 1206
FauI	CCCGC	1 cut(s)	33
FbaI	TGATCA	1 cut(s)	972
Fnu4HI	GCNGC	9 cut(s)	414, 690, 693, 696, 1086, 1296, 1359, 1362, 1365
FokI	GGATG	3 cut(s)	279, 1114, 1212
FriOI	GRGCYC	2 cut(s)	283, 1024
Fsp4HI	GCNGC	9 cut(s)	414, 690, 693, 696, 1086, 1296, 1359, 1362, 1365
GlaI	GCGC	1 cut(s)	1176
GluI	GCNGC	9 cut(s)	414, 690, 693, 696, 1086, 1296, 1359, 1362, 1365
HaeIII	GGCC	5 cut(s)	630, 698, 1070, 1345, 1554
HapII	CCGG	1 cut(s)	845
HgaI	GACGC	1 cut(s)	1578
HhaI	GCGC	1 cut(s)	1177
Hin1II	CATG	5 cut(s)	261, 319, 1020, 1092, 1210
Hin6I	GCGC	1 cut(s)	1175
HinP1I	GCGC	1 cut(s)	1175
HindIII	AAGCTT	1 cut(s)	966
HinfI	GANTC	7 cut(s)	217, 248, 493, 596, 945, 1007, 1403
HpaII	CCGG	1 cut(s)	845
HphI	GGTGA	4 cut(s)	38, 226, 464, 1198
Hpy188I	TCNGA	9 cut(s)	176, 498, 607, 720, 766, 944, 1006, 1290, 1389
Hpy188III	TCNNGA	6 cut(s)	196, 869, 955, 1235, 1417, 1430
HpyAV	CCTTC	3 cut(s)	270, 565, 1234
HpyCH4III	ACNGT	6 cut(s)	447, 453, 514, 678, 818, 1453
HpyF10VI	GCNNNNNNNGC	6 cut(s)	689, 695, 1114, 1342, 1358, 1364
HpyF3I	CTNAG	2 cut(s)	173, 344
Hsp92II	CATG	5 cut(s)	261, 319, 1020, 1092, 1210
HspAI	GCGC	1 cut(s)	1175
Ksp22I	TGATCA	1 cut(s)	972
KspI	CCGCGG	1 cut(s)	696
LmnI	GCTCC	4 cut(s)	505, 521, 1113, 1601
Lsp1109I	GCAGC	5 cut(s)	400, 701, 1307, 1348, 1370
LweI	GCATC	4 cut(s)	676, 924, 1095, 1345
MaeIII	GTNAC	5 cut(s)	62, 447, 1119, 1186, 1666
MbiI	CCGCTC	1 cut(s)	1604
MflI	RGATCY	1 cut(s)	1157
MhlI	GDGCHC	3 cut(s)	283, 526, 1024
MnlI	CCTC	7 cut(s)	119, 480, 641, 644, 880, 1092, 1313
Mph1103I	ATGCAT	2 cut(s)	669, 1338
MslI	CAYNNNNRTG	5 cut(s)	310, 529, 704, 1198, 1373
MspA1I	CMGCKG	4 cut(s)	683, 695, 1117, 1361
MspI	CCGG	1 cut(s)	845
MvnI	CGCG	3 cut(s)	40, 695, 1229
MwoI	GCNNNNNNNGC	6 cut(s)	689, 695, 1114, 1342, 1358, 1364
NlaIII	CATG	5 cut(s)	261, 319, 1020, 1092, 1210
NlaIV	GGNNCC	4 cut(s)	44, 891, 953, 1428
NmeAIII	GCCGAG	1 cut(s)	80
NmuCI	GTSAC	4 cut(s)	62, 447, 1119, 1186
NsiI	ATGCAT	2 cut(s)	669, 1338
PacI	TTAATTAA	1 cut(s)	1476
PaeR7I	CTCGAG	1 cut(s)	240
PceI	AGGCCT	1 cut(s)	630
PcsI	WCGNNNNNNNCGW	3 cut(s)	201, 573, 1233
PfeI	GAWTC	7 cut(s)	217, 248, 493, 596, 945, 1007, 1403
PflMI	CCANNNNNTGG	3 cut(s)	258, 1017, 1257
PkrI	GCNGC	9 cut(s)	415, 691, 694, 697, 1087, 1297, 1360, 1363, 1366
Ple19I	CGATCG	1 cut(s)	1004
PpuMI	RGGWCCY	1 cut(s)	43
PshBI	ATTAAT	3 cut(s)	657, 1476, 1536
Psp5II	RGGWCCY	1 cut(s)	43
PspN4I	GGNNCC	4 cut(s)	44, 891, 953, 1428
PspPI	GGNCC	3 cut(s)	43, 847, 1553
PspPPI	RGGWCCY	1 cut(s)	43
PspXI	VCTCGAGB	1 cut(s)	240
PsuI	RGATCY	1 cut(s)	1157
PvuI	CGATCG	1 cut(s)	1004
PvuII	CAGCTG	1 cut(s)	1361
RsaI	GTAC	4 cut(s)	455, 881, 1167, 1587
RsaNI	GTAC	4 cut(s)	454, 880, 1166, 1586
RseI	CAYNNNNRTG	5 cut(s)	310, 529, 704, 1198, 1373
SacII	CCGCGG	1 cut(s)	696
SatI	GCNGC	9 cut(s)	414, 690, 693, 696, 1086, 1296, 1359, 1362, 1365
Sau96I	GGNCC	3 cut(s)	43, 847, 1553
SduI	GDGCHC	3 cut(s)	283, 526, 1024
SfaNI	GCATC	4 cut(s)	676, 924, 1095, 1345
SfcI	CTRYAG	1 cut(s)	684
Sfr274I	CTCGAG	1 cut(s)	240
Sfr303I	CCGCGG	1 cut(s)	696
SgrBI	CCGCGG	1 cut(s)	696
SinI	GGWCC	2 cut(s)	43, 847
SlaI	CTCGAG	1 cut(s)	240
SmiMI	CAYNNNNRTG	5 cut(s)	310, 529, 704, 1198, 1373
SmlI	CTYRAG	1 cut(s)	240
SmoI	CTYRAG	1 cut(s)	240
SseBI	AGGCCT	1 cut(s)	630
SsiI	CCGC	9 cut(s)	40, 681, 693, 695, 1085, 1115, 1350, 1365, 1604
StuI	AGGCCT	1 cut(s)	630
TaaI	ACNGT	6 cut(s)	447, 453, 514, 678, 818, 1453
TaqI	TCGA	8 cut(s)	183, 195, 241, 462, 567, 930, 1000, 1236
TatI	WGTACW	1 cut(s)	1165
TauI	GCSGC	4 cut(s)	695, 698, 1088, 1367
TfiI	GAWTC	7 cut(s)	217, 248, 493, 596, 945, 1007, 1403
TscAI	CASTG	4 cut(s)	147, 399, 450, 531
TseFI	GTSAC	4 cut(s)	62, 447, 1119, 1186
TseI	GCWGC	5 cut(s)	413, 689, 1295, 1358, 1361
Tsp45I	GTSAC	4 cut(s)	62, 447, 1119, 1186
TspDTI	ATGAA	6 cut(s)	17, 281, 325, 554, 978, 1416
TspGWI	ACGGA	4 cut(s)	96, 1109, 1149, 1295
TspRI	CASTG	4 cut(s)	147, 399, 450, 531
Van91I	CCANNNNNTGG	3 cut(s)	258, 1017, 1257
VpaK11BI	GGWCC	2 cut(s)	43, 847
VspI	ATTAAT	3 cut(s)	657, 1476, 1536
XapI	RAATTY	8 cut(s)	185, 773, 872, 1265, 1283, 1390, 1438, 1657
XcmI	CCANNNNNNNNNTGG	1 cut(s)	988
XhoI	CTCGAG	1 cut(s)	240
Zsp2I	ATGCAT	2 cut(s)	669, 1338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.