pycom08g07030

Belongs to the cyclin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012580
Physical Location & Seq
Reverse (-)
5633879 .. 5635461
1583 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07030.1

Sequence Viewer

Length: 1146 bp
ATGCTTGTTGTAATACTATGTTTTACTCTGCGAAACCTGCAACCGTCAAAACGGTGTCACATCTCTGCATCCACTTTCACTATAAATATATTCAGTCGTATTCAGTCTTTGCTGGTCAACCATTTTCTCTCTCCAAATCTTTGGCTTCAGTACTGGGTTGGCTCACTGAATTCATACTATTTGATTCCTTTTTTCTACTACATTTTCTGCACCTTTCTTGCCAAGAAAGAAACAACGGAGGTCGACGGTGGTGATAACTACGATCCCTTCTCGCCGGACGCTTGTTGCCACGAAAGTCTAACATTTTTGGACGGAGAGATGGCAGATGAAAACGCATTGATCAGCACGAACTACTGCTCCGATAGTGCACTGGAACAAGAGCAACTGAAGAACTTAATCAACAGAGAGATGGAATTTGGGTTCAAGAAAGACGAAAATGTGATCATTCCCAGCTGGGTTTTGGAAGCTCGATCTGAATCCATCAAATGGGTTCTCAAGAGAAGTGCAGAACTTGGGTTTCAGCCCTTGACAGCATATCTGGCCATTGCATATTTCGATCGATTCTATTCGCTGAGTTCCGACCCTAAAGAAGAGCCAAAATCTGCAAAAAGGTTACTTTCGGTGGTGTGCCTGTCGTTGGCGGCAAAGATGGAGGAGTTGGACGTGAAGCTTCCACCACTGTCACAATACGCTGTCGGAGACTACACCTTTCCATGTCATTTCGTTGGGACGACGGAGTTGGCGTTTCTCGATCACTTAGATTGGAGGCTGAGTTTGATCACACCCTTTGCGTTTCTCGGTTACTTGATCTTCAAGTTATGCCACGGACCTGCATCAGATGTGAAGTCTCGGATTGAAGGACACCTCCTTTCTGGAATGACAGAGATTAACTTAATGCATCATCGACCGTCCGCCGTAGCAGCAGCCGCCACCTTAATGGCAATGGATCAGAAGTTAACAAAGGAAGCAGTGGAAGTCAAGATAAAATCGGTTCCTGACTTGAATTTTCTGGAAATTGAAGCTGTGTTTTTATGCTACAATCGGATGCAGGAGAAGGAGCTAATGAGTCTAAAGGACCGATCAATCCCGGTAAATGTTGCAGTCAGTTCTTCAGTTCCAGCAGAGAAGTCTGTAAAAACCGATTGA

Protein Analysis

382

Amino Acids

43.18

Weight (kDa)

5.47

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_N PF00134 150 - 258 5.8e-15 Cyclin, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 45, 838
AccB7I CCANNNNNTGG 1 cut(s) 486
AccI GTMKAC 1 cut(s) 243
AciI CCGC 3 cut(s) 641, 912, 927
AclWI GGATC 2 cut(s) 257, 954
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 3 cut(s) 169, 413, 1003
AcuI CTGAAG 3 cut(s) 131, 407, 1095
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 486, 637
AgsI TTSAA 5 cut(s) 424, 814, 857, 1003, 1019
AjiI CACGTC 1 cut(s) 664
AloI GAACNNNNNNTCC 4 cut(s) 341, 373, 404, 436
AluBI AGCT 5 cut(s) 453, 467, 670, 1022, 1060
AluI AGCT 5 cut(s) 453, 467, 670, 1022, 1060
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 2 cut(s) 693, 852
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 2 cut(s) 257, 954
AoxI GGCC 1 cut(s) 540
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 2 cut(s) 920, 923
ApoI RAATTY 3 cut(s) 169, 413, 1003
AspS9I GGNCC 2 cut(s) 827, 1075
AsuC2I CCSGG 1 cut(s) 1088
AsuHPI GGTGA 1 cut(s) 263
AvaII GGWCC 2 cut(s) 827, 1075
BaeGI GKGCMC 1 cut(s) 370
BalI TGGCCA 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 932, 935
BccI CCATC 4 cut(s) 313, 403, 488, 643
BceAI ACGGC 1 cut(s) 899
BclI TGATCA 3 cut(s) 339, 441, 777
BcnI CCSGG 1 cut(s) 1088
BcoDI GTCTC 2 cut(s) 693, 852
BfuAI ACCTGC 2 cut(s) 45, 838
BisI GCNGC 4 cut(s) 642, 921, 924, 927
BlsI GCNGC 4 cut(s) 643, 922, 925, 928
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 1088
Bme18I GGWCC 2 cut(s) 827, 1075
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 2 cut(s) 827, 1075
BmiI GGNNCC 1 cut(s) 993
BmrFI CCNGG 1 cut(s) 1088
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 4 cut(s) 77, 842, 907, 1035
BmuI ACTGGG 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 479
BpuMI CCSGG 1 cut(s) 1088
Bsa29I ATCGAT 1 cut(s) 559
BsaBI GATNNNNATC 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 823
BsaXI ACNNNNNCTCC 6 cut(s) 341, 371, 728, 757, 758, 787
Bsc4I CCNNNNNNNGG 2 cut(s) 486, 637
Bse1I ACTGG 2 cut(s) 158, 375
Bse3DI GCAATG 2 cut(s) 543, 948
Bse8I GATNNNNATC 1 cut(s) 475
BseCI ATCGAT 1 cut(s) 559
BseDI CCNNGG 1 cut(s) 823
BseGI GGATG 2 cut(s) 68, 1050
BseJI GATNNNNATC 1 cut(s) 475
BseLI CCNNNNNNNGG 2 cut(s) 486, 637
BseMI GCAATG 2 cut(s) 543, 948
BseMII CTCAG 2 cut(s) 563, 761
BseNI ACTGG 2 cut(s) 158, 375
BseRI GAGGAG 1 cut(s) 668
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 2 cut(s) 932, 935
BseYI CCCAGC 2 cut(s) 449, 453
BsgI GTGCAG 2 cut(s) 193, 525
Bsh1285I CGRYCG 2 cut(s) 559, 908
BshFI GGCC 1 cut(s) 542
BshVI ATCGAT 1 cut(s) 559
BsiEI CGRYCG 2 cut(s) 559, 908
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 2 cut(s) 275, 1088
BslFI GGGAC 1 cut(s) 742
BslI CCNNNNNNNGG 2 cut(s) 486, 637
BsmAI GTCTC 2 cut(s) 693, 852
BsmFI GGGAC 1 cut(s) 742
BsnI GGCC 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 370
BspACI CCGC 3 cut(s) 641, 912, 927
BspANI GGCC 1 cut(s) 542
BspCNI CTCAG 2 cut(s) 564, 762
BspDI ATCGAT 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 993
BspMI ACCTGC 2 cut(s) 45, 838
BspPI GGATC 2 cut(s) 257, 954
BspQI GCTCTTC 1 cut(s) 585
BsrDI GCAATG 2 cut(s) 543, 948
BsrI ACTGG 2 cut(s) 158, 375
BssECI CCNNGG 1 cut(s) 823
Bst4CI ACNGT 5 cut(s) 45, 54, 248, 681, 909
Bst6I CTCTTC 1 cut(s) 585
BstDEI CTNAG 3 cut(s) 572, 757, 770
BstDSI CCRYGG 1 cut(s) 823
BstF5I GGATG 2 cut(s) 68, 1050
BstMAI GTCTC 2 cut(s) 693, 852
BstMCI CGRYCG 2 cut(s) 559, 908
BstMWI GCNNNNNNNGC 4 cut(s) 37, 539, 920, 926
BstSCI CCNGG 1 cut(s) 1086
BstSLI GKGCMC 1 cut(s) 370
BstV1I GCAGC 2 cut(s) 932, 935
BstXI CCANNNNNNTGG 2 cut(s) 141, 937
Bsu15I ATCGAT 1 cut(s) 559
BsuRI GGCC 1 cut(s) 542
BsuTUI ATCGAT 1 cut(s) 559
BtgI CCRYGG 1 cut(s) 823
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 2 cut(s) 68, 1050
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 4 cut(s) 164, 368, 677, 975
BveI ACCTGC 2 cut(s) 45, 838
Cfr13I GGNCC 2 cut(s) 827, 1075
ClaI ATCGAT 1 cut(s) 559
CseI GACGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 151
CviAII CATG 1 cut(s) 714
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 3 cut(s) 572, 757, 770
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
EciI GGCGGA 1 cut(s) 901
Eco47I GGWCC 2 cut(s) 827, 1075
Eco57I CTGAAG 3 cut(s) 131, 407, 1095
EcoRI GAATTC 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 900
FaeI CATG 1 cut(s) 717
FaiI YATR 9 cut(s) 19, 83, 89, 175, 535, 550, 715, 820, 1033
FaqI GGGAC 1 cut(s) 742
FatI CATG 1 cut(s) 713
FbaI TGATCA 3 cut(s) 339, 441, 777
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 4 cut(s) 642, 921, 924, 927
FokI GGATG 2 cut(s) 55, 1057
Fsp4HI GCNGC 4 cut(s) 642, 921, 924, 927
GluI GCNGC 4 cut(s) 642, 921, 924, 927
GsaI CCCAGC 2 cut(s) 453, 457
HaeIII GGCC 1 cut(s) 542
HapII CCGG 2 cut(s) 275, 1088
HgaI GACGC 1 cut(s) 287
Hin1II CATG 1 cut(s) 717
HincII GTYRAC 3 cut(s) 118, 244, 957
HindII GTYRAC 3 cut(s) 118, 244, 957
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 4 cut(s) 184, 476, 561, 1066
HpaI GTTAAC 1 cut(s) 957
HpaII CCGG 2 cut(s) 275, 1088
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 4 cut(s) 118, 244, 368, 957
Hpy188I TCNGA 8 cut(s) 361, 475, 580, 698, 838, 852, 951, 1044
Hpy188III TCNNGA 7 cut(s) 424, 496, 749, 873, 979, 995, 1010
Hpy8I GTNNAC 4 cut(s) 118, 244, 368, 957
Hpy99I CGWCG 2 cut(s) 248, 736
HpyAV CCTTC 3 cut(s) 277, 851, 1048
HpyCH4III ACNGT 5 cut(s) 45, 54, 248, 681, 909
HpyCH4IV ACGT 1 cut(s) 663
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 539, 920, 926
HpyF3I CTNAG 3 cut(s) 572, 757, 770
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 1 cut(s) 717
Ksp22I TGATCA 3 cut(s) 339, 441, 777
KspAI GTTAAC 1 cut(s) 957
LguI GCTCTTC 1 cut(s) 585
LmnI GCTCC 2 cut(s) 362, 1057
Lsp1109I GCAGC 2 cut(s) 932, 935
LweI GCATC 4 cut(s) 77, 842, 907, 1035
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 4 cut(s) 56, 612, 681, 800
MboII GAAGA 4 cut(s) 400, 602, 802, 1101
MhlI GDGCHC 1 cut(s) 370
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 4 cut(s) 169, 413, 1003, 1014
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 1 cut(s) 1075
MmeI TCCRAC 3 cut(s) 603, 639, 676
MnlI CCTC 4 cut(s) 232, 646, 759, 875
Mox20I TGGCCA 1 cut(s) 542
Mph1103I ATGCAT 1 cut(s) 900
MscI TGGCCA 1 cut(s) 542
MseI TTAA 5 cut(s) 395, 888, 893, 935, 956
MslI CAYNNNNRTG 1 cut(s) 935
Msp20I TGGCCA 1 cut(s) 542
MspA1I CMGCKG 1 cut(s) 453
MspI CCGG 2 cut(s) 275, 1088
MspR9I CCNGG 1 cut(s) 1088
MwoI GCNNNNNNNGC 4 cut(s) 37, 539, 920, 926
NciI CCSGG 1 cut(s) 1088
NlaIII CATG 1 cut(s) 717
NlaIV GGNNCC 1 cut(s) 993
NmuCI GTSAC 2 cut(s) 56, 681
NsiI ATGCAT 1 cut(s) 900
PciSI GCTCTTC 1 cut(s) 585
PcsI WCGNNNNNNNCGW 1 cut(s) 740
PfeI GAWTC 3 cut(s) 184, 476, 561
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 4 cut(s) 643, 922, 925, 928
Ple19I CGATCG 1 cut(s) 559
PleI GAGTC 1 cut(s) 1074
PpsI GAGTC 1 cut(s) 1074
PspFI CCCAGC 2 cut(s) 449, 453
PspN4I GGNNCC 1 cut(s) 993
PspPI GGNCC 2 cut(s) 827, 1075
PvuI CGATCG 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 453
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 935
SalI GTCGAC 1 cut(s) 242
SapI GCTCTTC 1 cut(s) 585
SaqAI TTAA 5 cut(s) 395, 888, 893, 935, 956
SatI GCNGC 4 cut(s) 642, 921, 924, 927
Sau96I GGNCC 2 cut(s) 827, 1075
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 1 cut(s) 1075
ScrFI CCNGG 1 cut(s) 1088
SduI GDGCHC 1 cut(s) 370
SfaNI GCATC 4 cut(s) 77, 842, 907, 1035
SinI GGWCC 2 cut(s) 827, 1075
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 4 cut(s) 169, 413, 1003, 1014
SsiI CCGC 3 cut(s) 641, 912, 927
StyD4I CCNGG 1 cut(s) 1086
TaaI ACNGT 5 cut(s) 45, 54, 248, 681, 909
TaiI ACGT 1 cut(s) 666
TaqI TCGA 6 cut(s) 243, 469, 555, 559, 750, 904
TaqII GACCGA 1 cut(s) 1092
TasI AATT 4 cut(s) 169, 413, 1003, 1014
TatI WGTACW 1 cut(s) 150
TauI GCSGC 2 cut(s) 644, 929
TfiI GAWTC 3 cut(s) 184, 476, 561
Tru1I TTAA 5 cut(s) 395, 888, 893, 935, 956
Tru9I TTAA 5 cut(s) 395, 888, 893, 935, 956
TscAI CASTG 4 cut(s) 171, 375, 684, 975
TseFI GTSAC 2 cut(s) 56, 681
TseI GCWGC 2 cut(s) 920, 923
Tsp45I GTSAC 2 cut(s) 56, 681
TspDTI ATGAA 2 cut(s) 162, 342
TspGWI ACGGA 4 cut(s) 251, 327, 749, 840
TspRI CASTG 4 cut(s) 171, 375, 684, 975
Van91I CCANNNNNTGG 1 cut(s) 486
VneI GTGCAC 1 cut(s) 366
VpaK11BI GGWCC 2 cut(s) 827, 1075
XapI RAATTY 3 cut(s) 169, 413, 1003
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 243
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.