Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006442

Physical Location & Seq

Genomic Coordinates Forward (+)

8925428 .. 8931986

Gene Structure 6559 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1123200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3219 bp

ATGGAAGCCAATGCGGGACTGGTGGCCGGCTCTTACAAGAGGAACGAGCTCGTTCGGATTCGCCACGATTCAGATAGCGCGCCCAAACCCGTAAAGAATCTGAATGGGCAGATATGTCAGATCTGTGGTGATACCGTTGGAGTTACAGCCACCGGTGATGTCTTTGTTGCCTGCAATGAGTGTGCCTTTCCAGTCTGTCGACCTTGTTATGAGTATGAGCGGAAAGATGGGAACCAGGCTTGCCCACAGTGCAAGACTAGATATAAAAGGCACAAAGGGAGTCCTCGAGTGGATGGAGATGATGATGAGGATGACATTGATGATCTGGAGAATGAGTTCAATTATGCGCAAGGAACTAGCAATGCCAGACGCCAATGGCAAGGAGAAGATGCTGATCTCTCATCTTCCTCGAGACATGAATCTCAACAGCCGATCCCCCTTCTCACGAATGGGCAGTCGGTGTCTGGTGAGATTCCCTGTGCCACACCTGATAACCAATCTGTGCGAACTACATCAGGTCCTTTGGATCCGCGACAGCCAGTTCCAGTAAGAATAGTGGACCCATCAAAGGACTTGAATTCTTATGGGCTAGGAAATGTTGACTGGAAAGAAAGGGTTGAAGGCTGGAAACTCAAACAGGACAAAAATATGATGCATATGACTAATAAATACGCTGAAGGAAAGGGGGACATGGAAGGCACTGGTTCAAATGGTGAAGAACTCCAAATGGCTGATGATGCTAGACAACCTTTGAGTCGCATCGTGCCTATTTCTTCATCTCATCTGACACCTTATCGTGTTGTGATCATACTCCGGCTTATTATTCTGGGATTCTTCTTGCAATACCGTGCAACTCACCCAGTGAAAGATGCATACCCATTATGGTTAACATCAGTCATCTGTGAGATTTGGTTTGCTTTATCCTGGCTTCTGGATCAGTTTCCAAAATGGTTTCCCATTAACCGTGAGACCTATCTTGATAGGCTCGCACTGAGGTATGATCGTGATGGAGAGCCATCCCAGTTAGCTCCCATAGATGTGTTTGTCAGTACAGTGGATCCCATGAAAGAGCCTCCTCTGATTACGGCTAACACTGTTTTGTCCATACTTGCTGTGGATTACCCAGTGGACAAAGTCTCTTGCTATGTTTCAGATGATGGGTCAGCAATGCTTACATTTGAATCTCTTTCTGAAACTGCGGAGTTTGCTAGGAAGTGGGTACCTTTTTGCAAGAAACACAACATTGAACCTAGAGCCCCTGAGTTTTATTTTGCCCAAAAGATTGATTACTTAAAGGACAAGATACAGCCTTCTTTTGTAAAAGAGCGTAGAGCAATGAAGAGAGAGTATGAAGAATTCAAGGTTCGGATCAATGCCTTAGTTGCCAAGGCACAGAAAATGCCTGAAGAAGGTTGGACAATGCAGGATGGGACTCCATGGCCTGGGAACAACTCTAGGGACCATCCAGGAATGATCCAGGTTTTCTTAGGCCACAGTGGGGGCCTTGATACTGATGGCAATGAGCTGCCTCGACTTGTTTATGTTTCTCGTGAGAAGCGACCTGGATTCCAGCATCACAAAAAAGCTGGAGCAATGAATGCATTGATTCGAGTTTCAGCCGTCCTGACAAATGGTGCATATCTCTTGAATGTCGATTGTGATCACTACTTCAATAATAGTAAAGCTCTTAAGGAAGCCATGTGTTTCATGATGGACCCAGCTTATGGGAAGAAGACCTGCTATGTCCAGTTCCCTCAGCGATTTGATGGCATTGATTTGCACGATAGATATGCCAATCGGAACATTGTCTTCTTTGATATCAACTTGAAAGGGCTGGATGGCATCCAGGGCCCAGTCTATGTGGGAACTGGTTGTTGTTTCAACAGACAAGCTCTATATGGGTACGATCCGGTTTTGACTGAGGAAGATTTGCAACCAAACATTATTGTCAAGAGTTGTTGTGGTTCAAGAAAGAAGAATAAGACTAGCAATAAGAAGTATATTGACAAGAAAAGGGCGGTTAAAAGAACGGAATCCACCATTCCCATTTTTAATATGGAAGACATTGAAGAGGGTGTTGAAGGTTATGATGATGAGAGGACTCTTCTTATGTCCCAGAAGAGCTTAGAGAAGCGTTTTGGTCAATCACCAGTTTTTATTGCAGCCACATTCATGGAACAAGGAGGCATTCCGCCTACAACCAATCCTGCAACTCTTTTGAAGGAAGCCATACATGTTATTAGCTGTGGATATGAAGACAAGAGTGAATGGGGAAAAGAGATTGGATGGATCTATGGCTCTGTGACTGAAGATATTTTAACTGGGTTTAAGATGCATGCTCGTGGTTGGATATCAGTCTATTGCATGCCTCCCCGCCCAGCATTTAAGGGCTCTGCTCCCATCAATCTTTCTGATCGTTTGAACCAAGTGCTTCGATGGGCCTTGGGTTCCATTGAAATTTTGCTCAGCAGGCATTGTCCCATATGGTATGGCTACAATGGCAAGTTGAAACTTTTGGAGAGACTTGCATACATCAATACCATCGTTTACCCCCTCACATCTATACCACTGATTGCATACTGTTTACTTCCTGCCTTTTGCCTTCTCACAGAAAAGTTTATCATTCCTGAGATAAGTAATTTCGCTAGCATGTGGTTCATTCTGCTCTTTGTTTCTATTATTGCAACCGGTATCCTTGAGCTAAGGTGGAGTGGAGTTAGTATTGAAGACTGGTGGAGAAACGAACAGTTCTGGATCATTGGTGGAACATCAGCTCATCTCTTCGCTGTCTTCCAAGGTCTCCTGAAAGTTCTTGCTGGGATTGATACCAACTTCACTGTGACGTCAAAGGCATCTGATGAGGATGGGGATTTTGCTGAGCTGTACGTGTTCAAATGGACATCACTTCTCATCCCTCCAACCACGGTTCTTCTTGTGAACATGGTAGGTATTGTGGCCGGTGTTTCATATGCCATAAATAGCGGTTACCAATCTTGGGGTCCACTCTTCGGCAAACTGTTTTTCGCCCTATGGGTCGTAGCACATCTGTATCCATTCTTGAAGGGTCTGTTGGGTCGGCAAAATCGCACCCCTACAATCGTCATCGTGTGGTCTATTCTTCTTGCATCAATATTTTCCTTGTTGTGGGTGCGCATCGACCCATTCACTTCGGACAAGACAAAAGCTGCATCAAACGGTCAATGTGGCATCAACTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0006950 GO:0006970 GO:0006972 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009628 GO:0009651 GO:0009832 GO:0009833 GO:0009987 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0022402 GO:0030243 GO:0030244 GO:0031410 GO:0031982 GO:0031984 GO:0033692 GO:0034637 GO:0034645 GO:0042538 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046527 GO:0050896 GO:0051273 GO:0051274 GO:0051301 GO:0061640 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0097708 GO:0098791 GO:1901576 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-UDP

Protein Analysis

1073

Amino Acids

120.85

Weight (kDa)

6.37

Isoelectric Point (pI)

40.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UDP	PF14569	29 - 105	2.2e-44	Zinc-binding RING-finger
Cellulose_synt	PF03552	345 - 1063	0.0	Cellulose synthase
Glyco_trans_2_3	PF13632	761 - 866	2.9e-07	Glycosyl transferase family group 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006442)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32410
fragaria_vesca	FvH4_2g29420 FvH4_2g29420 FvH4_2g29420 FvH4_2g29420 FvH4_2g29420 FvH4_2g29420 FvH4_2g29420 FvH4_2g29420
malus_domestica	MD08G1147200.v1.1 MD15G1123200.v1.1
prunus_persica	Prupe.1G478600_v2.0.a1 Prupe.1G478600_v2.0.a1
pyrus_communis	pycom08g12420 pycom15g11100
rosa_chinensis	RchiOBHm_Chr6g0298221
rosa_laevigata	RLG00000011525
rosa_multiflora	Rmu_sc0017507.1_g000003
rosa_roxburghii	Rroxscaffold_7G00169850
rosa_rugosa	Rorug06G0279600 Rorug06G0279600
rosa_samantha	Rh6AG389900 Rh6BG398300 Rh6CG404200 Rh6DG390900
rosa_wichuraiana	Rw6G034040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2846
Acc16I	TGCGCA	2 cut(s)	348, 3152
Acc36I	ACCTGC	1 cut(s)	1745
Acc65I	GGTACC	1 cut(s)	1219
AccB1I	GGYRCC	1 cut(s)	1219
AccB7I	CCANNNNNTGG	2 cut(s)	1442, 1724
AccBSI	CCGCTC	1 cut(s)	220
AccI	GTMKAC	1 cut(s)	199
AccII	CGCG	2 cut(s)	80, 532
AciI	CCGC	8 cut(s)	14, 220, 530, 1199, 2018, 2192, 2374, 2982
AcoI	YGGCCR	2 cut(s)	24, 2955
AcsI	RAATTY	3 cut(s)	577, 1355, 2457
AcuI	CTGAAG	3 cut(s)	696, 1425, 2328
AcyI	GRCGYC	2 cut(s)	370, 2843
AdeI	CACNNNGTG	1 cut(s)	862
AfaI	GTAC	4 cut(s)	1051, 1221, 1904, 2885
AfiI	CCNNNNNNNGG	8 cut(s)	568, 1409, 1442, 1498, 1724, 2995, 3008, 3144
AflII	CTTAAG	1 cut(s)	1688
AflIII	ACRYGT	2 cut(s)	2233, 2886
AgeI	ACCGGT	2 cut(s)	152, 2687
AjnI	CCWGG	7 cut(s)	234, 923, 1441, 1465, 1476, 1561, 1845
AjuI	GAANNNNNNNTTGG	2 cut(s)	3053, 3085
AloI	GAACNNNNNNTCC	2 cut(s)	320, 352
Alw21I	GWGCWC	1 cut(s)	51
Alw26I	GTCTC	5 cut(s)	406, 962, 1141, 2515, 2804
Ama87I	CYCGRG	2 cut(s)	285, 409
AoxI	GGCC	7 cut(s)	24, 1439, 1489, 1501, 1849, 2439, 2955
ApaI	GGGCCC	1 cut(s)	1853
ApeKI	GCWGC	3 cut(s)	1525, 2162, 3185
ApoI	RAATTY	3 cut(s)	577, 1355, 2457
ArsI	GACNNNNNNTTYG	2 cut(s)	440, 472
AsiGI	ACCGGT	2 cut(s)	152, 2687
Asp700I	GAANNNNTTC	1 cut(s)	335
Asp718I	GGTACC	1 cut(s)	1219
AspLEI	GCGC	4 cut(s)	80, 82, 349, 3153
AspS9I	GGNCC	9 cut(s)	518, 559, 1459, 1501, 1714, 1849, 1850, 2439, 2999
AsuHPI	GGTGA	6 cut(s)	140, 167, 479, 725, 848, 2139
AsuNHI	GCTAGC	1 cut(s)	2645
AvaI	CYCGRG	2 cut(s)	285, 409
AvaII	GGWCC	5 cut(s)	518, 559, 1459, 1714, 2999
BaeGI	GKGCMC	1 cut(s)	1853
BaeI	ACNNNNGTAYC	2 cut(s)	3032, 3065
BamHI	GGATCC	2 cut(s)	526, 1057
BanI	GGYRCC	1 cut(s)	1219
BanII	GRGCYC	4 cut(s)	51, 1258, 1853, 2393
BauI	CACGAG	2 cut(s)	1548, 2340
BbsI	GAAGAC	6 cut(s)	1739, 1801, 2067, 2262, 2733, 2782
Bbv12I	GWGCWC	1 cut(s)	51
BbvCI	CCTCAGC	1 cut(s)	1755
BbvI	GCAGC	3 cut(s)	1512, 2174, 3172
BceAI	ACGGC	2 cut(s)	1101, 1604
BcgI	CGANNNNNNTGC	2 cut(s)	1772, 1806
BciT130I	CCWGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
BciVI	GTATCC	2 cut(s)	2702, 3060
BclI	TGATCA	2 cut(s)	804, 1660
BcoDI	GTCTC	5 cut(s)	406, 962, 1141, 2515, 2804
BfaI	CTAG	9 cut(s)	258, 357, 590, 741, 1209, 1251, 1455, 1986, 2646
BfrI	CTTAAG	1 cut(s)	1688
BfuAI	ACCTGC	1 cut(s)	1745
BfuI	GTATCC	2 cut(s)	2702, 3060
BglII	AGATCT	1 cut(s)	120
BisI	GCNGC	3 cut(s)	1526, 2163, 3186
BlpI	GCTNAGC	2 cut(s)	2465, 2877
BlsI	GCNGC	3 cut(s)	1527, 2164, 3187
Bme1390I	CCNGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
Bme18I	GGWCC	5 cut(s)	518, 559, 1459, 1714, 2999
BmeT110I	CYCGRG	2 cut(s)	285, 409
BmgT120I	GGNCC	9 cut(s)	518, 559, 1459, 1501, 1714, 1849, 1850, 2439, 2999
BmrFI	CCNGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
BmrI	ACTGGG	5 cut(s)	854, 1015, 1118, 1847, 2331
BmtI	GCTAGC	1 cut(s)	2649
BmuI	ACTGGG	5 cut(s)	854, 1015, 1118, 1847, 2331
BpiI	GAAGAC	6 cut(s)	1739, 1801, 2067, 2262, 2733, 2782
BpmI	CTGGAG	2 cut(s)	347, 1608
Bpu10I	CCTNAGC	2 cut(s)	1755, 2702
Bpu1102I	GCTNAGC	2 cut(s)	2465, 2877
BpuEI	CTTGAG	1 cut(s)	2717
BsaAI	YACGTR	1 cut(s)	2887
BsaBI	GATNNNNATC	2 cut(s)	393, 1659
BsaHI	GRCGYC	2 cut(s)	370, 2843
BsaI	GGTCTC	2 cut(s)	962, 2804
BsaJI	CCNNGG	7 cut(s)	1386, 1436, 1442, 1846, 2442, 2794, 2922
BsaWI	WCCGGW	3 cut(s)	152, 1909, 2687
BsaXI	ACNNNNNCTCC	2 cut(s)	320, 350
Bsc4I	CCNNNNNNNGG	8 cut(s)	568, 1409, 1442, 1498, 1724, 2995, 3008, 3144
Bse118I	RCCGGY	4 cut(s)	26, 152, 2687, 2957
Bse3DI	GCAATG	6 cut(s)	181, 367, 1173, 1341, 1525, 1599
Bse8I	GATNNNNATC	2 cut(s)	393, 1659
BseBI	CCWGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
BseDI	CCNNGG	7 cut(s)	1386, 1436, 1442, 1846, 2442, 2794, 2922
BseJI	GATNNNNATC	2 cut(s)	393, 1659
BseLI	CCNNNNNNNGG	8 cut(s)	568, 1409, 1442, 1498, 1724, 2995, 3008, 3144
BseMI	GCAATG	6 cut(s)	181, 367, 1173, 1341, 1525, 1599
BseMII	CTCAG	7 cut(s)	983, 1251, 1769, 1911, 2479, 2619, 2868
BsePI	GCGCGC	1 cut(s)	78
BseRI	GAGGAG	1 cut(s)	1065
BseSI	GKGCMC	1 cut(s)	1853
BseXI	GCAGC	3 cut(s)	1512, 2174, 3172
BseYI	CCCAGC	3 cut(s)	1717, 2377, 2816
Bsh1236I	CGCG	2 cut(s)	80, 532
BshFI	GGCC	7 cut(s)	26, 1441, 1491, 1503, 1851, 2441, 2957
BshNI	GGYRCC	1 cut(s)	1219
BshTI	ACCGGT	2 cut(s)	152, 2687
BsiHKAI	GWGCWC	1 cut(s)	51
BsiHKCI	CYCGRG	2 cut(s)	285, 409
BsiSI	CCGG	6 cut(s)	27, 153, 814, 1910, 2688, 2958
BslFI	GGGAC	6 cut(s)	30, 701, 1444, 1472, 2098, 2463
BslI	CCNNNNNNNGG	8 cut(s)	568, 1409, 1442, 1498, 1724, 2995, 3008, 3144
BsmAI	GTCTC	5 cut(s)	406, 962, 1141, 2515, 2804
BsmFI	GGGAC	6 cut(s)	30, 701, 1444, 1472, 2098, 2463
BsmI	GAATGC	2 cut(s)	1603, 2187
BsnI	GGCC	7 cut(s)	26, 1441, 1491, 1503, 1851, 2441, 2957
Bso31I	GGTCTC	2 cut(s)	962, 2804
BsoBI	CYCGRG	2 cut(s)	285, 409
Bsp120I	GGGCCC	1 cut(s)	1849
Bsp1286I	GDGCHC	4 cut(s)	51, 1258, 1853, 2393
Bsp1720I	GCTNAGC	2 cut(s)	2465, 2877
Bsp19I	CCATGG	1 cut(s)	1436
BspACI	CCGC	8 cut(s)	14, 220, 530, 1199, 2018, 2192, 2374, 2982
BspANI	GGCC	7 cut(s)	26, 1441, 1491, 1503, 1851, 2441, 2957
BspCNI	CTCAG	7 cut(s)	984, 1252, 1768, 1912, 2478, 2620, 2869
BspFNI	CGCG	2 cut(s)	80, 532
BspHI	TCATGA	1 cut(s)	1707
BspMI	ACCTGC	1 cut(s)	1745
BspOI	GCTAGC	1 cut(s)	2649
BspQI	GCTCTTC	1 cut(s)	2114
BspT107I	GGYRCC	1 cut(s)	1219
BspTI	CTTAAG	1 cut(s)	1688
BspTNI	GGTCTC	2 cut(s)	962, 2804
BsrBI	CCGCTC	1 cut(s)	220
BsrDI	GCAATG	6 cut(s)	181, 367, 1173, 1341, 1525, 1599
BsrFI	RCCGGY	4 cut(s)	26, 152, 2687, 2957
BssAI	RCCGGY	4 cut(s)	26, 152, 2687, 2957
BssECI	CCNNGG	7 cut(s)	1386, 1436, 1442, 1846, 2442, 2794, 2922
BssHII	GCGCGC	1 cut(s)	78
BssNI	GRCGYC	2 cut(s)	370, 2843
BssSI	CACGAG	2 cut(s)	1548, 2340
BssT1I	CCWWGG	4 cut(s)	1386, 1436, 2442, 2794
Bst2BI	CACGAG	2 cut(s)	1548, 2340
Bst2UI	CCWGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
Bst6I	CTCTTC	6 cut(s)	1334, 2064, 2109, 2114, 2786, 3011
BstACI	GRCGYC	2 cut(s)	370, 2843
BstAFI	CTTAAG	1 cut(s)	1688
BstAPI	GCANNNNNTGC	1 cut(s)	1598
BstBAI	YACGTR	1 cut(s)	2887
BstC8I	GCNNGC	9 cut(s)	28, 80, 172, 241, 987, 2337, 2366, 2471, 2647
BstDSI	CCRYGG	2 cut(s)	1436, 2922
BstEII	GGTNACC	1 cut(s)	2984
BstENI	CCTNNNNNAGG	1 cut(s)	1407
BstFNI	CGCG	2 cut(s)	80, 532
BstHHI	GCGC	4 cut(s)	80, 82, 349, 3153
BstMAI	GTCTC	5 cut(s)	406, 962, 1141, 2515, 2804
BstMWI	GCNNNNNNNGC	8 cut(s)	249, 737, 1205, 1382, 1598, 1848, 2470, 2499
BstNI	CCWGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
BstNSI	RCATGY	4 cut(s)	2237, 2339, 2368, 2653
BstPI	GGTNACC	1 cut(s)	2984
BstSCI	CCNGG	7 cut(s)	234, 923, 1441, 1465, 1476, 1561, 1845
BstSLI	GKGCMC	1 cut(s)	1853
BstUI	CGCG	2 cut(s)	80, 532
BstV1I	GCAGC	3 cut(s)	1512, 2174, 3172
BstV2I	GAAGAC	6 cut(s)	1739, 1801, 2067, 2262, 2733, 2782
BstX2I	RGATCY	4 cut(s)	120, 526, 1057, 2289
BstXI	CCANNNNNNTGG	1 cut(s)	2173
BstYI	RGATCY	4 cut(s)	120, 526, 1057, 2289
BsuI	GTATCC	2 cut(s)	2702, 3060
BsuRI	GGCC	7 cut(s)	26, 1441, 1491, 1503, 1851, 2441, 2957
BtgI	CCRYGG	2 cut(s)	1436, 2922
BveI	ACCTGC	1 cut(s)	1745
Cac8I	GCNNGC	9 cut(s)	28, 80, 172, 241, 987, 2337, 2366, 2471, 2647
CciI	TCATGA	1 cut(s)	1707
CfoI	GCGC	4 cut(s)	80, 82, 349, 3153
Cfr10I	RCCGGY	4 cut(s)	26, 152, 2687, 2957
Cfr13I	GGNCC	9 cut(s)	518, 559, 1459, 1501, 1714, 1849, 1850, 2439, 2999
CseI	GACGC	1 cut(s)	378
Csp6I	GTAC	4 cut(s)	1050, 1220, 1903, 2884
CspAI	ACCGGT	2 cut(s)	152, 2687
CviQI	GTAC	4 cut(s)	1050, 1220, 1903, 2884
DraIII	CACNNNGTG	1 cut(s)	862
EaeI	YGGCCR	2 cut(s)	24, 2955
Eam1104I	CTCTTC	6 cut(s)	1334, 2064, 2109, 2114, 2786, 3011
EarI	CTCTTC	6 cut(s)	1334, 2064, 2109, 2114, 2786, 3011
EciI	GGCGGA	1 cut(s)	2181
Ecl136II	GAGCTC	1 cut(s)	49
Eco130I	CCWWGG	4 cut(s)	1386, 1436, 2442, 2794
Eco24I	GRGCYC	4 cut(s)	51, 1258, 1853, 2393
Eco31I	GGTCTC	2 cut(s)	962, 2804
Eco32I	GATATC	2 cut(s)	1819, 2352
Eco47I	GGWCC	5 cut(s)	518, 559, 1459, 1714, 2999
Eco53kI	GAGCTC	1 cut(s)	49
Eco57I	CTGAAG	3 cut(s)	696, 1425, 2328
Eco88I	CYCGRG	2 cut(s)	285, 409
Eco91I	GGTNACC	1 cut(s)	2984
EcoICRI	GAGCTC	1 cut(s)	49
EcoNI	CCTNNNNNAGG	1 cut(s)	1407
EcoO109I	RGGNCCY	3 cut(s)	518, 1501, 1849
EcoO65I	GGTNACC	1 cut(s)	2984
EcoRI	GAATTC	2 cut(s)	577, 1355
EcoRII	CCWGG	7 cut(s)	234, 923, 1441, 1465, 1476, 1561, 1845
EcoRV	GATATC	2 cut(s)	1819, 2352
EcoT14I	CCWWGG	4 cut(s)	1386, 1436, 2442, 2794
EcoT22I	ATGCAT	4 cut(s)	657, 874, 1603, 2337
EcoT38I	GRGCYC	4 cut(s)	51, 1258, 1853, 2393
ErhI	CCWWGG	4 cut(s)	1386, 1436, 2442, 2794
FaqI	GGGAC	6 cut(s)	30, 701, 1444, 1472, 2098, 2463
FauI	CCCGC	2 cut(s)	7, 2381
FauNDI	CATATG	3 cut(s)	657, 2483, 2968
FbaI	TGATCA	2 cut(s)	804, 1660
FblI	GTMKAC	1 cut(s)	199
Fnu4HI	GCNGC	3 cut(s)	1526, 2163, 3186
FriOI	GRGCYC	4 cut(s)	51, 1258, 1853, 2393
Fsp4HI	GCNGC	3 cut(s)	1526, 2163, 3186
FspAI	RTGCGCAY	1 cut(s)	3152
FspBI	CTAG	9 cut(s)	258, 357, 590, 741, 1209, 1251, 1455, 1986, 2646
FspI	TGCGCA	2 cut(s)	348, 3152
GlaI	GCGC	4 cut(s)	79, 81, 348, 3152
GluI	GCNGC	3 cut(s)	1526, 2163, 3186
GsaI	CCCAGC	3 cut(s)	1721, 2381, 2820
GsuI	CTGGAG	2 cut(s)	347, 1608
HaeIII	GGCC	7 cut(s)	26, 1441, 1491, 1503, 1851, 2441, 2957
HapII	CCGG	6 cut(s)	27, 153, 814, 1910, 2688, 2958
HgaI	GACGC	1 cut(s)	378
HhaI	GCGC	4 cut(s)	80, 82, 349, 3153
Hin1I	GRCGYC	2 cut(s)	370, 2843
Hin6I	GCGC	4 cut(s)	78, 80, 347, 3151
HinP1I	GCGC	4 cut(s)	78, 80, 347, 3151
HincII	GTYRAC	3 cut(s)	200, 601, 888
HindII	GTYRAC	3 cut(s)	200, 601, 888
HpaI	GTTAAC	1 cut(s)	888
HpaII	CCGG	6 cut(s)	27, 153, 814, 1910, 2688, 2958
HphI	GGTGA	6 cut(s)	140, 167, 479, 725, 848, 2139
Hpy166II	GTNNAC	9 cut(s)	200, 559, 601, 888, 1129, 2548, 2585, 2938, 3002
Hpy8I	GTNNAC	9 cut(s)	200, 559, 601, 888, 1129, 2548, 2585, 2938, 3002
HpyCH4IV	ACGT	2 cut(s)	2843, 2886
HpyF10VI	GCNNNNNNNGC	8 cut(s)	249, 737, 1205, 1382, 1598, 1848, 2470, 2499
HpySE526I	ACGT	2 cut(s)	2843, 2886
Hsp92I	GRCGYC	2 cut(s)	370, 2843
HspAI	GCGC	4 cut(s)	78, 80, 347, 3151
KpnI	GGTACC	1 cut(s)	1223
KroI	GCCGGC	1 cut(s)	26
KroNI	GCCGGC	1 cut(s)	28
Ksp22I	TGATCA	2 cut(s)	804, 1660
KspAI	GTTAAC	1 cut(s)	888
LguI	GCTCTTC	1 cut(s)	2114
LmnI	GCTCC	3 cut(s)	1033, 1589, 2401
Lsp1109I	GCAGC	3 cut(s)	1512, 2174, 3172
MaeI	CTAG	9 cut(s)	258, 357, 590, 741, 1209, 1251, 1455, 1986, 2646
MaeII	ACGT	2 cut(s)	2843, 2886
MaeIII	GTNAC	4 cut(s)	142, 2302, 2839, 2984
MbiI	CCGCTC	1 cut(s)	220
MflI	RGATCY	4 cut(s)	120, 526, 1057, 2289
MhlI	GDGCHC	4 cut(s)	51, 1258, 1853, 2393
MluCI	AATT	5 cut(s)	340, 577, 1355, 2457, 2638
MlyI	GAGTC	4 cut(s)	289, 763, 1426, 2095
MmeI	TCCRAC	4 cut(s)	118, 1394, 2327, 2942
Mph1103I	ATGCAT	4 cut(s)	657, 874, 1603, 2337
MroNI	GCCGGC	1 cut(s)	26
MroXI	GAANNNNTTC	1 cut(s)	335
MseI	TTAA	9 cut(s)	887, 960, 1292, 1689, 2022, 2052, 2318, 2328, 2385
MslI	CAYNNNNRTG	3 cut(s)	1037, 2171, 2340
MspCI	CTTAAG	1 cut(s)	1688
MspI	CCGG	6 cut(s)	27, 153, 814, 1910, 2688, 2958
MspR9I	CCNGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
Mva1269I	GAATGC	2 cut(s)	1603, 2187
MvaI	CCWGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
MvnI	CGCG	2 cut(s)	80, 532
MwoI	GCNNNNNNNGC	8 cut(s)	249, 737, 1205, 1382, 1598, 1848, 2470, 2499
NaeI	GCCGGC	1 cut(s)	28
NcoI	CCATGG	1 cut(s)	1436
NdeI	CATATG	3 cut(s)	657, 2483, 2968
NgoMIV	GCCGGC	1 cut(s)	26
NheI	GCTAGC	1 cut(s)	2645
NmuCI	GTSAC	2 cut(s)	2302, 2839
NsbI	TGCGCA	2 cut(s)	348, 3152
NsiI	ATGCAT	4 cut(s)	657, 874, 1603, 2337
NspI	RCATGY	4 cut(s)	2237, 2339, 2368, 2653
PaeI	GCATGC	2 cut(s)	2339, 2368
PaeR7I	CTCGAG	2 cut(s)	285, 409
PagI	TCATGA	1 cut(s)	1707
PauI	GCGCGC	1 cut(s)	78
PciI	ACATGT	1 cut(s)	2233
PciSI	GCTCTTC	1 cut(s)	2114
PcsI	WCGNNNNNNNCGW	1 cut(s)	1555
PctI	GAATGC	2 cut(s)	1603, 2187
PdiI	GCCGGC	1 cut(s)	28
PdmI	GAANNNNTTC	1 cut(s)	335
PflFI	GACNNNGTC	1 cut(s)	1133
PflMI	CCANNNNNTGG	2 cut(s)	1442, 1724
PfoI	TCCNGGA	1 cut(s)	1465
PinAI	ACCGGT	2 cut(s)	152, 2687
PkrI	GCNGC	3 cut(s)	1527, 2164, 3187
PleI	GAGTC	4 cut(s)	288, 762, 1426, 2095
PpsI	GAGTC	4 cut(s)	288, 762, 1426, 2095
Ppu21I	YACGTR	1 cut(s)	2887
PpuMI	RGGWCCY	1 cut(s)	518
PscI	ACATGT	1 cut(s)	2233
Psp124BI	GAGCTC	1 cut(s)	51
Psp5II	RGGWCCY	1 cut(s)	518
Psp6I	CCWGG	7 cut(s)	234, 923, 1441, 1465, 1476, 1561, 1845
PspEI	GGTNACC	1 cut(s)	2984
PspFI	CCCAGC	3 cut(s)	1717, 2377, 2816
PspGI	CCWGG	7 cut(s)	234, 923, 1441, 1465, 1476, 1561, 1845
PspOMI	GGGCCC	1 cut(s)	1849
PspPI	GGNCC	9 cut(s)	518, 559, 1459, 1501, 1714, 1849, 1850, 2439, 2999
PspPPI	RGGWCCY	1 cut(s)	518
PspXI	VCTCGAGB	1 cut(s)	285
PsuI	RGATCY	4 cut(s)	120, 526, 1057, 2289
PsyI	GACNNNGTC	1 cut(s)	1133
PteI	GCGCGC	1 cut(s)	78
RsaI	GTAC	4 cut(s)	1051, 1221, 1904, 2885
RsaNI	GTAC	4 cut(s)	1050, 1220, 1903, 2884
RseI	CAYNNNNRTG	3 cut(s)	1037, 2171, 2340
SacI	GAGCTC	1 cut(s)	51
SalI	GTCGAC	1 cut(s)	198
SapI	GCTCTTC	1 cut(s)	2114
SaqAI	TTAA	9 cut(s)	887, 960, 1292, 1689, 2022, 2052, 2318, 2328, 2385
SatI	GCNGC	3 cut(s)	1526, 2163, 3186
Sau96I	GGNCC	9 cut(s)	518, 559, 1459, 1501, 1714, 1849, 1850, 2439, 2999
SchI	GAGTC	4 cut(s)	289, 763, 1426, 2095
ScrFI	CCNGG	7 cut(s)	236, 925, 1443, 1467, 1478, 1563, 1847
SduI	GDGCHC	4 cut(s)	51, 1258, 1853, 2393
Sfr274I	CTCGAG	2 cut(s)	285, 409
SgrAI	CRCCGGYG	1 cut(s)	152
SinI	GGWCC	5 cut(s)	518, 559, 1459, 1714, 2999
SlaI	CTCGAG	2 cut(s)	285, 409
SmiMI	CAYNNNNRTG	3 cut(s)	1037, 2171, 2340
SmlI	CTYRAG	4 cut(s)	285, 409, 1688, 2696
SmoI	CTYRAG	4 cut(s)	285, 409, 1688, 2696
SphI	GCATGC	2 cut(s)	2339, 2368
Sse9I	AATT	5 cut(s)	340, 577, 1355, 2457, 2638
SsiI	CCGC	8 cut(s)	14, 220, 530, 1199, 2018, 2192, 2374, 2982
SspI	AATATT	1 cut(s)	3132
SspMI	CTAG	9 cut(s)	258, 357, 590, 741, 1209, 1251, 1455, 1986, 2646
SstI	GAGCTC	1 cut(s)	51
StyD4I	CCNGG	7 cut(s)	234, 923, 1441, 1465, 1476, 1561, 1845
StyI	CCWWGG	4 cut(s)	1386, 1436, 2442, 2794
TaiI	ACGT	2 cut(s)	2846, 2889
TaqI	TCGA	8 cut(s)	199, 286, 410, 1531, 1609, 1653, 2434, 3156
TasI	AATT	5 cut(s)	340, 577, 1355, 2457, 2638
TatI	WGTACW	1 cut(s)	1049
Tru1I	TTAA	9 cut(s)	887, 960, 1292, 1689, 2022, 2052, 2318, 2328, 2385
Tru9I	TTAA	9 cut(s)	887, 960, 1292, 1689, 2022, 2052, 2318, 2328, 2385
TseFI	GTSAC	2 cut(s)	2302, 2839
TseI	GCWGC	3 cut(s)	1525, 2162, 3185
Tsp45I	GTSAC	2 cut(s)	2302, 2839
TspGWI	ACGGA	1 cut(s)	2045
Tth111I	GACNNNGTC	1 cut(s)	1133
Van91I	CCANNNNNTGG	2 cut(s)	1442, 1724
Vha464I	CTTAAG	1 cut(s)	1688
VpaK11BI	GGWCC	5 cut(s)	518, 559, 1459, 1714, 2999
XagI	CCTNNNNNAGG	1 cut(s)	1407
XapI	RAATTY	3 cut(s)	577, 1355, 2457
XceI	RCATGY	4 cut(s)	2237, 2339, 2368, 2653
XcmI	CCANNNNNNNNNTGG	3 cut(s)	16, 1111, 2053
XhoI	CTCGAG	2 cut(s)	285, 409
XmiI	GTMKAC	1 cut(s)	199
XmnI	GAANNNNTTC	1 cut(s)	335
XspI	CTAG	9 cut(s)	258, 357, 590, 741, 1209, 1251, 1455, 1986, 2646
ZraI	GACGTC	1 cut(s)	2844
Zsp2I	ATGCAT	4 cut(s)	657, 874, 1603, 2337

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.