Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005734

Physical Location & Seq

Genomic Coordinates Forward (+)

12211775 .. 12221594

Gene Structure 9820 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1162100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGGCGTCGAAGCTGAAGATTGCAGGGACATGGGCGGGCGTGCTGGAGGTTGAATTGGAGAATTGGACCGTGCCCATGTTGCGAGACGAAGTTGCAAAACGATCAAACTGCGACCCCAATTCGATCAATCTGATTTGCGCGGGCAGGGTCTTGAAAGACGGCGGCGGCGATGAGAAACTCGCCCAAGTGGGTATCAAGAACAACGCCAAGATTCTTGCCAGTCGGGTGTGCGCGGAGGAGGGCAAGTCGTTGAAGGAGGAGTTGATGGCTGAGGAGGAACGCTCCCGAAGATTGACTCGAGTCAAGGCAGCTGCCACTGCATTGGCCAAGAGACACGCAGATGGATCATTGCCGATTGAAGACTTCAATATAGTTCTTGAAGATCAAAGTGGAAAGAAAGTAGAGTTGGGATCCGAGACTGATCAGAGGGCAATTATGATGGGCCTGATGCTTCATACGAATGCAAAGCAACTAATTCAAAGACAAAATTACAAAGACGCGTTAGAAGTGCTTACCATGGGAGAGGAGGCTTTCTCTCTTTGTGACCCGAAGGTTATTGAGCTTGTTGACAATCCTCCTATACTCCAAATCGATATGGTTTGGTGCTATTTCATGCTCCGAGATATCAGCTGGCTTGCAGTGGCAGGAGTTCGCCTTGAAAAAGCCAGAAAAGGTATTGAGAGAGCTCATGGAAAGGACTCTTCTCGTGTCAGACTCCTTCAGGGAGGTCGCTATCCAGAACTTGCACTATATTTGAGACTGGAGCTACTGGAAGGGGTGGTTGCGTATCATAGTGGCCAGTACGATAAATCAAGGAAGGTGCTGACTTCTGCACAAGAAAAATTCACCAAGCTACAAGTGCCAGATGAAGCTCTATCACTTGTTATGAGCATGGGATTTAAAGAACGAGATTCTAAAAGGGCATTGCGGATGAGCAATCAAGATGTATCTAGTGCTGTTGATTTTCTTGTTGAGCAGAAGGCAAAGAGAGCCCAAAAACGGGAAGAAGATATTCAGCGACGAAATGAAATTATGGAGCAAAAGAAGTATGGGACAACACCATTGAAGAAAGCTGTGGATATCGAGAAATTGAACCAATTGGTATCCATTGGTTTTGAGAAGGAGCTTGCTGCTGAATCACTTAGAAGAAACGAGAATGATGCTGAGAAGGCATTGGATGATTTGACAAATCCAGAAGTTAATTCTTCCATACAGGTATACATTGAATCAAAGAAAAGGAAACGACAGCGCCAAGCAATTGATTCTTCAATTGAATCGCTTGTAGGCATGGGGTTTGAAAGATCAAGAGTGATTGAAGCTTTTCAGACTGCTGGTGATGGGGCAACACTGGAGCAAGCATTGCATCAGTTGCTCGCAGGAAATGCAACAGACCCCACAAATGCTGCCAACAACCAGTCTCAGGCGATTCCCACAGCTGCCGCTGACAGCAATATCCCTGCTGATGTTGCTAATGATATTGTGAATTCTCTTGTTTCAAGGCTAGATAATGCTGATCAAAACGGCGAAGCTGGAGGTCCATCAGCTACTAGTGAAGAACGTGATGCAGAAATGGAAGATGCACTCGCTGGAGAACTGGCACAAGGAGATGCATTCTCAGACTATGACCTTGAAGTTACTGAAGAAGGTGCGGCCATAAGCGAGTATTTGGCTTTGCTGGACTCGACAGGCAGAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0019222 GO:0019538 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:2000058 GO:2000060

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UBA ubiquitin

Protein Analysis

566

Amino Acids

62.37

Weight (kDa)

5.07

Isoelectric Point (pI)

46.22

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005734)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G12550
fragaria_vesca	FvH4_1g01990 FvH4_1g01990 FvH4_1g01990 FvH4_3g33700
malus_domestica	MD15G1162100.v1.1
prunus_persica	Prupe.7G257500_v2.0.a1
pyrus_communis	pycom15g14580
rosa_chinensis	RchiOBHm_Chr2g0087051 RchiOBHm_Chr5g0061231
rosa_laevigata	RLG00000015824 RLG00000035444
rosa_multiflora	Rmu_sc0007612.1_g000046 Rmu_sc0010698.1_g000014
rosa_roxburghii	Rroxscaffold_1G00019470 Rroxscaffold_2G00153940
rosa_rugosa	Rorug01G0471700.1 Rorug05G0339000
rosa_samantha	Rh2AG025200 Rh2BG025000 Rh2CG025500 Rh2DG025400 Rh5BG413000 Rh5CG437500
rosa_wichuraiana	Rw0G022890 Rw0G023050 Rw2G002000 Rw5G037700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1218
AccII	CGCG	3 cut(s)	140, 233, 500
AciI	CCGC	8 cut(s)	35, 140, 162, 165, 233, 928, 1440, 1649
AclWI	GGATC	3 cut(s)	352, 405, 418
AcoI	YGGCCR	3 cut(s)	324, 796, 1650
AcsI	RAATTY	2 cut(s)	842, 1483
AcuI	CTGAAG	3 cut(s)	35, 704, 1659
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	803
AfiI	CCNNNNNNNGG	3 cut(s)	999, 1000, 1420
AflIII	ACRYGT	1 cut(s)	498
AhlI	ACTAGT	1 cut(s)	1547
AjuI	GAANNNNNNNTTGG	2 cut(s)	389, 421
Alw21I	GWGCWC	1 cut(s)	688
Alw26I	GTCTC	5 cut(s)	78, 325, 410, 751, 1422
AlwI	GGATC	3 cut(s)	352, 405, 418
Ama87I	CYCGRG	1 cut(s)	297
AoxI	GGCC	4 cut(s)	324, 442, 796, 1650
ApeKI	GCWGC	5 cut(s)	308, 311, 1130, 1403, 1436
ApoI	RAATTY	2 cut(s)	842, 1483
Asp700I	GAANNNNTTC	2 cut(s)	1011, 1320
AspLEI	GCGC	3 cut(s)	140, 233, 1251
AspS9I	GGNCC	3 cut(s)	66, 442, 1535
AsuHPI	GGTGA	2 cut(s)	838, 1346
AvaI	CYCGRG	1 cut(s)	297
AvaII	GGWCC	2 cut(s)	66, 1535
BaeGI	GKGCMC	1 cut(s)	75
BalI	TGGCCA	2 cut(s)	326, 798
BamHI	GGATCC	1 cut(s)	410
BanII	GRGCYC	2 cut(s)	688, 994
BauI	CACGAG	1 cut(s)	705
BbsI	GAAGAC	1 cut(s)	366
Bbv12I	GWGCWC	1 cut(s)	688
BbvCI	CCTCAGC	1 cut(s)	270
BbvI	GCAGC	5 cut(s)	298, 320, 1117, 1390, 1423
BccI	CCATC	5 cut(s)	259, 335, 433, 1331, 1546
BceAI	ACGGC	2 cut(s)	175, 1537
BcgI	CGANNNNNNTGC	4 cut(s)	90, 124, 1142, 1176
BciVI	GTATCC	1 cut(s)	1114
BclI	TGATCA	2 cut(s)	421, 1513
BcoDI	GTCTC	5 cut(s)	78, 325, 410, 751, 1422
BcuI	ACTAGT	1 cut(s)	1547
BfaI	CTAG	3 cut(s)	951, 1502, 1548
BfoI	RGCGCY	1 cut(s)	1252
BfuI	GTATCC	1 cut(s)	1114
BisI	GCNGC	9 cut(s)	163, 166, 309, 312, 1131, 1404, 1437, 1440, 1650
BlsI	GCNGC	9 cut(s)	164, 167, 310, 313, 1132, 1405, 1438, 1441, 1651
Bme18I	GGWCC	2 cut(s)	66, 1535
BmeT110I	CYCGRG	1 cut(s)	297
BmgT120I	GGNCC	3 cut(s)	66, 442, 1535
BmiI	GGNNCC	1 cut(s)	412
BmsI	GCATC	6 cut(s)	438, 1150, 1372, 1552, 1567, 1597
BoxI	GACNNNNGTC	1 cut(s)	299
BpiI	GAAGAC	1 cut(s)	366
BplI	GAGNNNNNCTC	4 cut(s)	266, 298, 518, 550
BpmI	CTGGAG	5 cut(s)	65, 782, 1370, 1551, 1608
Bpu10I	CCTNAGC	1 cut(s)	270
Bsa29I	ATCGAT	1 cut(s)	591
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	1 cut(s)	516
BsaXI	ACNNNNNCTCC	2 cut(s)	230, 260
Bsc4I	CCNNNNNNNGG	3 cut(s)	999, 1000, 1420
Bse1I	ACTGG	7 cut(s)	219, 765, 774, 799, 1353, 1414, 1599
Bse3DI	GCAATG	3 cut(s)	347, 923, 1358
BseCI	ATCGAT	1 cut(s)	591
BseDI	CCNNGG	1 cut(s)	516
BseGI	GGATG	2 cut(s)	936, 1183
BseLI	CCNNNNNNNGG	3 cut(s)	999, 1000, 1420
BseMI	GCAATG	3 cut(s)	347, 923, 1358
BseMII	CTCAG	4 cut(s)	261, 1155, 1433, 1629
BseNI	ACTGG	7 cut(s)	219, 765, 774, 799, 1353, 1414, 1599
BseRI	GAGGAG	4 cut(s)	251, 272, 287, 539
BseSI	GKGCMC	1 cut(s)	75
BseXI	GCAGC	5 cut(s)	298, 320, 1117, 1390, 1423
BsgI	GTGCAG	1 cut(s)	816
Bsh1236I	CGCG	3 cut(s)	140, 233, 500
BshFI	GGCC	4 cut(s)	326, 444, 798, 1652
BshVI	ATCGAT	1 cut(s)	591
BsiHKAI	GWGCWC	1 cut(s)	688
BsiHKCI	CYCGRG	1 cut(s)	297
BslFI	GGGAC	2 cut(s)	40, 1066
BslI	CCNNNNNNNGG	3 cut(s)	999, 1000, 1420
BsmAI	GTCTC	5 cut(s)	78, 325, 410, 751, 1422
BsmBI	CGTCTC	1 cut(s)	78
BsmFI	GGGAC	2 cut(s)	40, 1066
BsmI	GAATGC	2 cut(s)	466, 1610
BsnI	GGCC	4 cut(s)	326, 444, 798, 1652
BsoBI	CYCGRG	1 cut(s)	297
Bsp1286I	GDGCHC	3 cut(s)	75, 688, 994
Bsp143I	GATC	8 cut(s)	101, 123, 344, 382, 410, 421, 1301, 1513
Bsp19I	CCATGG	1 cut(s)	516
BspACI	CCGC	8 cut(s)	35, 140, 162, 165, 233, 928, 1440, 1649
BspANI	GGCC	4 cut(s)	326, 444, 798, 1652
BspCNI	CTCAG	4 cut(s)	262, 1156, 1432, 1628
BspDI	ATCGAT	1 cut(s)	591
BspFNI	CGCG	3 cut(s)	140, 233, 500
BspLI	GGNNCC	1 cut(s)	412
BspPI	GGATC	3 cut(s)	352, 405, 418
BsrDI	GCAATG	3 cut(s)	347, 923, 1358
BsrI	ACTGG	7 cut(s)	219, 765, 774, 799, 1353, 1414, 1599
BssECI	CCNNGG	1 cut(s)	516
BssMI	GATC	8 cut(s)	101, 123, 344, 382, 410, 421, 1301, 1513
BssNAI	GTATAC	1 cut(s)	1219
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	705
BssT1I	CCWWGG	1 cut(s)	516
Bst1107I	GTATAC	1 cut(s)	1219
Bst2BI	CACGAG	1 cut(s)	705
Bst4CI	ACNGT	1 cut(s)	70
Bst6I	CTCTTC	1 cut(s)	706
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	3 cut(s)	1360, 1369, 1382
BstC8I	GCNNGC	8 cut(s)	37, 41, 142, 632, 636, 1128, 1356, 1374
BstDEI	CTNAG	5 cut(s)	270, 1142, 1164, 1419, 1615
BstDSI	CCRYGG	1 cut(s)	516
BstF5I	GGATG	2 cut(s)	936, 1183
BstFNI	CGCG	3 cut(s)	140, 233, 500
BstH2I	RGCGCY	1 cut(s)	1252
BstHHI	GCGC	3 cut(s)	140, 233, 1251
BstKTI	GATC	8 cut(s)	104, 126, 347, 385, 413, 424, 1304, 1516
BstMAI	GTCTC	5 cut(s)	78, 325, 410, 751, 1422
BstMBI	GATC	8 cut(s)	101, 123, 344, 382, 410, 421, 1301, 1513
BstMWI	GCNNNNNNNGC	9 cut(s)	79, 317, 859, 989, 1169, 1360, 1369, 1382, 1466
BstPAI	GACNNNNGTC	1 cut(s)	299
BstSLI	GKGCMC	1 cut(s)	75
BstUI	CGCG	3 cut(s)	140, 233, 500
BstV1I	GCAGC	5 cut(s)	298, 320, 1117, 1390, 1423
BstV2I	GAAGAC	1 cut(s)	366
BstX2I	RGATCY	1 cut(s)	410
BstXI	CCANNNNNNTGG	1 cut(s)	322
BstYI	RGATCY	1 cut(s)	410
BstZ17I	GTATAC	1 cut(s)	1219
Bsu15I	ATCGAT	1 cut(s)	591
BsuI	GTATCC	1 cut(s)	1114
BsuRI	GGCC	4 cut(s)	326, 444, 798, 1652
BsuTUI	ATCGAT	1 cut(s)	591
BtgI	CCRYGG	1 cut(s)	516
BtgZI	GCGATG	1 cut(s)	183
BtsCI	GGATG	2 cut(s)	936, 1183
BtsI	GCAGTG	2 cut(s)	315, 645
BtsIMutI	CAGTG	3 cut(s)	315, 645, 1346
Cac8I	GCNNGC	8 cut(s)	37, 41, 142, 632, 636, 1128, 1356, 1374
CfoI	GCGC	3 cut(s)	140, 233, 1251
Cfr13I	GGNCC	3 cut(s)	66, 442, 1535
ClaI	ATCGAT	1 cut(s)	591
CseI	GACGC	1 cut(s)	506
Csp6I	GTAC	1 cut(s)	802
CviAII	CATG	7 cut(s)	30, 76, 517, 613, 689, 892, 1288
CviQI	GTAC	1 cut(s)	802
DdeI	CTNAG	5 cut(s)	270, 1142, 1164, 1419, 1615
DpnI	GATC	8 cut(s)	103, 125, 346, 384, 412, 423, 1303, 1515
DpnII	GATC	8 cut(s)	101, 123, 344, 382, 410, 421, 1301, 1513
DraI	TTTAAA	1 cut(s)	901
EaeI	YGGCCR	3 cut(s)	324, 796, 1650
Eam1104I	CTCTTC	1 cut(s)	706
EarI	CTCTTC	1 cut(s)	706
Ecl136II	GAGCTC	1 cut(s)	686
Eco130I	CCWWGG	1 cut(s)	516
Eco24I	GRGCYC	2 cut(s)	688, 994
Eco32I	GATATC	2 cut(s)	625, 1081
Eco47I	GGWCC	2 cut(s)	66, 1535
Eco53kI	GAGCTC	1 cut(s)	686
Eco57I	CTGAAG	3 cut(s)	35, 704, 1659
Eco88I	CYCGRG	1 cut(s)	297
EcoICRI	GAGCTC	1 cut(s)	686
EcoRI	GAATTC	1 cut(s)	1483
EcoRV	GATATC	2 cut(s)	625, 1081
EcoT14I	CCWWGG	1 cut(s)	516
EcoT22I	ATGCAT	1 cut(s)	1612
EcoT38I	GRGCYC	2 cut(s)	688, 994
ErhI	CCWWGG	1 cut(s)	516
Esp3I	CGTCTC	1 cut(s)	78
FaeI	CATG	7 cut(s)	33, 79, 520, 616, 692, 895, 1291
FaqI	GGGAC	2 cut(s)	40, 1066
FatI	CATG	7 cut(s)	29, 75, 516, 612, 688, 891, 1287
FauI	CCCGC	2 cut(s)	28, 133
FbaI	TGATCA	2 cut(s)	421, 1513
FblI	GTMKAC	1 cut(s)	1218
Fnu4HI	GCNGC	9 cut(s)	163, 166, 309, 312, 1131, 1404, 1437, 1440, 1650
FokI	GGATG	2 cut(s)	943, 1190
FriOI	GRGCYC	2 cut(s)	688, 994
Fsp4HI	GCNGC	9 cut(s)	163, 166, 309, 312, 1131, 1404, 1437, 1440, 1650
FspBI	CTAG	3 cut(s)	951, 1502, 1548
GlaI	GCGC	3 cut(s)	139, 232, 1250
GluI	GCNGC	9 cut(s)	163, 166, 309, 312, 1131, 1404, 1437, 1440, 1650
GsuI	CTGGAG	5 cut(s)	65, 782, 1370, 1551, 1608
HaeII	RGCGCY	1 cut(s)	1252
HaeIII	GGCC	4 cut(s)	326, 444, 798, 1652
HgaI	GACGC	1 cut(s)	506
HhaI	GCGC	3 cut(s)	140, 233, 1251
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	7 cut(s)	33, 79, 520, 616, 692, 895, 1291
Hin6I	GCGC	3 cut(s)	138, 231, 1249
HinP1I	GCGC	3 cut(s)	138, 231, 1249
HincII	GTYRAC	1 cut(s)	568
HindII	GTYRAC	1 cut(s)	568
HindIII	AAGCTT	1 cut(s)	1317
HphI	GGTGA	2 cut(s)	838, 1346
Hpy166II	GTNNAC	2 cut(s)	568, 1219
Hpy188I	TCNGA	7 cut(s)	132, 415, 426, 620, 713, 1326, 1618
Hpy188III	TCNNGA	9 cut(s)	151, 196, 285, 377, 737, 941, 1084, 1193, 1305
Hpy8I	GTNNAC	2 cut(s)	568, 1219
Hpy99I	CGWCG	2 cut(s)	10, 1023
HpyAV	CCTTC	9 cut(s)	247, 544, 728, 767, 811, 973, 1114, 1162, 1637
HpyCH4III	ACNGT	1 cut(s)	70
HpyCH4IV	ACGT	1 cut(s)	1558
HpyF10VI	GCNNNNNNNGC	9 cut(s)	79, 317, 859, 989, 1169, 1360, 1369, 1382, 1466
HpyF3I	CTNAG	5 cut(s)	270, 1142, 1164, 1419, 1615
HpySE526I	ACGT	1 cut(s)	1558
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	7 cut(s)	33, 79, 520, 616, 692, 895, 1291
HspAI	GCGC	3 cut(s)	138, 231, 1249
Ksp22I	TGATCA	2 cut(s)	421, 1513
Kzo9I	GATC	8 cut(s)	101, 123, 344, 382, 410, 421, 1301, 1513
LmnI	GCTCC	6 cut(s)	287, 621, 763, 1036, 1123, 1351
Lsp1109I	GCAGC	5 cut(s)	298, 320, 1117, 1390, 1423
LweI	GCATC	6 cut(s)	438, 1150, 1372, 1552, 1567, 1597
MaeI	CTAG	3 cut(s)	951, 1502, 1548
MaeII	ACGT	1 cut(s)	1558
MaeIII	GTNAC	2 cut(s)	542, 1633
MalI	GATC	8 cut(s)	103, 125, 346, 384, 412, 423, 1303, 1515
MboI	GATC	8 cut(s)	101, 123, 344, 382, 410, 421, 1301, 1513
MfeI	CAATTG	3 cut(s)	1097, 1257, 1269
MflI	RGATCY	1 cut(s)	410
MhlI	GDGCHC	3 cut(s)	75, 688, 994
MlsI	TGGCCA	2 cut(s)	326, 798
MluI	ACGCGT	1 cut(s)	498
MluNI	TGGCCA	2 cut(s)	326, 798
MlyI	GAGTC	5 cut(s)	289, 309, 692, 708, 1673
Mox20I	TGGCCA	2 cut(s)	326, 798
Mph1103I	ATGCAT	1 cut(s)	1612
MroXI	GAANNNNTTC	2 cut(s)	1011, 1320
MscI	TGGCCA	2 cut(s)	326, 798
MseI	TTAA	2 cut(s)	900, 1200
MslI	CAYNNNNRTG	2 cut(s)	339, 459
Msp20I	TGGCCA	2 cut(s)	326, 798
MspA1I	CMGCKG	4 cut(s)	311, 630, 1436, 1442
MunI	CAATTG	3 cut(s)	1097, 1257, 1269
Mva1269I	GAATGC	2 cut(s)	466, 1610
MvnI	CGCG	3 cut(s)	140, 233, 500
MwoI	GCNNNNNNNGC	9 cut(s)	79, 317, 859, 989, 1169, 1360, 1369, 1382, 1466
NcoI	CCATGG	1 cut(s)	516
NdeII	GATC	8 cut(s)	101, 123, 344, 382, 410, 421, 1301, 1513
NlaIII	CATG	7 cut(s)	33, 79, 520, 616, 692, 895, 1291
NlaIV	GGNNCC	1 cut(s)	412
NmuCI	GTSAC	1 cut(s)	542
NsiI	ATGCAT	1 cut(s)	1612
PaeR7I	CTCGAG	1 cut(s)	297
PcsI	WCGNNNNNNNCGW	1 cut(s)	165
PctI	GAATGC	2 cut(s)	466, 1610
PdmI	GAANNNNTTC	2 cut(s)	1011, 1320
PfeI	GAWTC	7 cut(s)	211, 911, 1136, 1226, 1262, 1274, 1426
PkrI	GCNGC	9 cut(s)	164, 167, 310, 313, 1132, 1405, 1438, 1441, 1651
PleI	GAGTC	5 cut(s)	289, 308, 692, 708, 1673
PpsI	GAGTC	5 cut(s)	289, 308, 692, 708, 1673
PshAI	GACNNNNGTC	1 cut(s)	299
Psp124BI	GAGCTC	1 cut(s)	688
PspN4I	GGNNCC	1 cut(s)	412
PspPI	GGNCC	3 cut(s)	66, 442, 1535
PspXI	VCTCGAGB	1 cut(s)	297
PsuI	RGATCY	1 cut(s)	410
PvuII	CAGCTG	3 cut(s)	311, 630, 1436
RsaI	GTAC	1 cut(s)	803
RsaNI	GTAC	1 cut(s)	802
RseI	CAYNNNNRTG	2 cut(s)	339, 459
SacI	GAGCTC	1 cut(s)	688
SaqAI	TTAA	2 cut(s)	900, 1200
SatI	GCNGC	9 cut(s)	163, 166, 309, 312, 1131, 1404, 1437, 1440, 1650
Sau3AI	GATC	8 cut(s)	101, 123, 344, 382, 410, 421, 1301, 1513
Sau96I	GGNCC	3 cut(s)	66, 442, 1535
SchI	GAGTC	5 cut(s)	289, 309, 692, 708, 1673
SduI	GDGCHC	3 cut(s)	75, 688, 994
SfaNI	GCATC	6 cut(s)	438, 1150, 1372, 1552, 1567, 1597
Sfr274I	CTCGAG	1 cut(s)	297
SinI	GGWCC	2 cut(s)	66, 1535
SlaI	CTCGAG	1 cut(s)	297
SmiMI	CAYNNNNRTG	2 cut(s)	339, 459
SmlI	CTYRAG	1 cut(s)	297
SmoI	CTYRAG	1 cut(s)	297
SpeI	ACTAGT	1 cut(s)	1547
SsiI	CCGC	8 cut(s)	35, 140, 162, 165, 233, 928, 1440, 1649
SspMI	CTAG	3 cut(s)	951, 1502, 1548
SstI	GAGCTC	1 cut(s)	688
StyI	CCWWGG	1 cut(s)	516
TaaI	ACNGT	1 cut(s)	70
TaiI	ACGT	1 cut(s)	1561
TaqI	TCGA	6 cut(s)	8, 122, 298, 591, 1083, 1682
TauI	GCSGC	4 cut(s)	165, 168, 1442, 1652
TfiI	GAWTC	7 cut(s)	211, 911, 1136, 1226, 1262, 1274, 1426
Tru1I	TTAA	2 cut(s)	900, 1200
Tru9I	TTAA	2 cut(s)	900, 1200
TscAI	CASTG	3 cut(s)	322, 645, 1353
TseFI	GTSAC	1 cut(s)	542
TseI	GCWGC	5 cut(s)	308, 311, 1130, 1403, 1436
Tsp45I	GTSAC	1 cut(s)	542
TspDTI	ATGAA	4 cut(s)	443, 601, 882, 1041
TspRI	CASTG	3 cut(s)	322, 645, 1353
VpaK11BI	GGWCC	2 cut(s)	66, 1535
XapI	RAATTY	2 cut(s)	842, 1483
XhoI	CTCGAG	1 cut(s)	297
XmiI	GTMKAC	1 cut(s)	1218
XmnI	GAANNNNTTC	2 cut(s)	1011, 1320
XspI	CTAG	3 cut(s)	951, 1502, 1548
Zsp2I	ATGCAT	1 cut(s)	1612

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.