Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0005734

Physical Location & Seq

Genomic Coordinates Forward (+)

1523527 .. 1527337

Gene Structure 3811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG025400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1683 bp

ATGGCGAAAGTAAAGATTGCAGGGACATGGGCGGGTGTGCTGGAGGTCGAACTGGACAATTGGACGGTGCCCATGTTGAGAGAAGAGGTTGCAAAGCGATCCAACTGCGAGCCCGGCTCGATCAACTTGATATGCGCCGGCCGAGTTTTGAAGGACGGCGATGGCAGCGAGAATTTGACCCAACTGGGTGTTAAGAACAACGCAAAGATTCTGGCTAGTCGGGTTTCTGCTCAGGAGGGTAAGTCTTTGGGTCAGGAGCTGATGGCCGAAAGCGAACGCGCTCAGAGACTCGCTCGAGTCAAGGCTGCTGCAACTGCGCTCTCCAAGAGACATGCTGATGGTTCATTGCCAATTGAAGATTTCAATATAGTACTTGAAGATCAGAGTGGAAAGAAAGTACAAATGGGATCGGAGACTGATCAGCGGGCAATCATGATGGGCCTGATGCTCCATACCAATGCAAAGCAATTGATTAAAGCACAAAATTACCAAGATGCATTGGAAGTGCTCACCATGGGAGAGGAGGCTTTCTCTCTTTGTGATCCGAAGGTTATTGAGCTGGTTGATAATCCTTCTATACTGCAAATCGATATGGTTTGGTGTTATTTTATGCTTAGAGACATCAACTGGCTCTCAGTGGCAGGCTTTCGCCTTGAGAAAGCCAGAGAAGGACTTGAACGAGCTCATGGAAAGGACTCTTCTCGTGTTAGACTCCTTCAGGAAGGTCGCTATCCAGAGCGTGCACTATATTTGAGATTGGAGCTGCTGGAAGGGGTGGTGGCCTATCACAGTGGTCAGGTTAATAAGTCAATGAAGGTGTTGACTTCTGCACAAGAGAAGTTCACCCAGCTTCAGGTGCCGGATGAATCTCTTTCTCTTGTGATGAGCATGGGCTTCAGAGAGCGAGATGCAAAAAGGGCACTGAGGATGAGCAATCAAGATGTTTCCAGTGCTGTTGATTTTCTTGTTGAGCAGAAAGCAAAGACAGCCCAAAAACGGGAAGATGATATCCGGCGGCGAGATGAAATTAAGGAGCAAAAGAAGTATGGTACTACACCCCTGAAGAAAGCTGTGGATATCGAGAAATTGAACCAACTGGTCTCCATTGGGTTTGAAAAGGAGCTTGCTGCTGAAGCACTACGAAGAAATGAGAATGACAGTGAGAAGGCATTGGATGATTTGACAAATCCAGAGACAAATTCTTCCATACAGCAACATCTTGAATCAAGGAAAAGTAAACGACAGCGCCAAGCAGTAGATGCTTCAGTTGAAGCTCTTGTACGCATGGGCTTTGACAGATCATTAGCGGTCGAAGCTTTGCAGGGTGGAGGGACAATGGACCAAGCAATCCGTCGACTGCTCTCGGGACAGGCACCAAACCCCACAAATGCTGCTGACCAACCTGAGGCAATTCCTGCAGTCGATGCTAACAACAATAACCAATCTGATGCTGCTACTGACATTGCAAATTCTCTTGCTTCAATGCTTGATGATCAAAATGGTGAAGTCGGAGTTCCTTCAACATCTAGTAATGCATCAGAGCGTGATGCAGAAATGGAAAGCGAACTTGCTGAAGAACTAGCACAAGCAGATGCTTTCACTGACTATGACATCGAAGTTACTAAAGAAGGTGAAGCCATAAGTGAGTACTTGGCTCTACTGAATTCAGCGGGAACAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0019222 GO:0019538 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:2000058 GO:2000060

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UBA ubiquitin

Protein Analysis

560

Amino Acids

61.79

Weight (kDa)

5.03

Isoelectric Point (pI)

41.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UBA_7	PF22562	291 - 325	9.1e-06	UBA-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005734)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G12550
fragaria_vesca	FvH4_1g01990 FvH4_1g01990 FvH4_1g01990 FvH4_3g33700
malus_domestica	MD15G1162100.v1.1
prunus_persica	Prupe.7G257500_v2.0.a1
pyrus_communis	pycom15g14580
rosa_chinensis	RchiOBHm_Chr2g0087051 RchiOBHm_Chr5g0061231
rosa_laevigata	RLG00000015824 RLG00000035444
rosa_multiflora	Rmu_sc0007612.1_g000046 Rmu_sc0010698.1_g000014
rosa_roxburghii	Rroxscaffold_1G00019470 Rroxscaffold_2G00153940
rosa_rugosa	Rorug01G0471700.1 Rorug05G0339000
rosa_samantha	Rh2AG025200 Rh2BG025000 Rh2CG025500 Rh2DG025400 Rh5BG413000 Rh5CG437500
rosa_wichuraiana	Rw0G022890 Rw0G023050 Rw2G002000 Rw5G037700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	67, 856, 1372
AccI	GTMKAC	1 cut(s)	1354
AccII	CGCG	1 cut(s)	279
AciI	CCGC	5 cut(s)	32, 424, 1015, 1307, 1670
AclWI	GGATC	3 cut(s)	93, 415, 536
AcoI	YGGCCR	2 cut(s)	139, 264
AcsI	RAATTY	4 cut(s)	172, 1198, 1468, 1663
AcuI	CTGAAG	7 cut(s)	701, 836, 880, 1082, 1152, 1248, 1593
AfaI	GTAC	5 cut(s)	372, 399, 1051, 1281, 1649
AfiI	CCNNNNNNNGG	4 cut(s)	853, 996, 997, 1405
AluBI	AGCT	9 cut(s)	259, 559, 683, 763, 850, 1070, 1123, 1274, 1316
AluI	AGCT	9 cut(s)	259, 559, 683, 763, 850, 1070, 1123, 1274, 1316
Alw21I	GWGCWC	3 cut(s)	510, 685, 745
Alw26I	GTCTC	6 cut(s)	280, 322, 407, 612, 1105, 1187
Alw44I	GTGCAC	1 cut(s)	741
AlwI	GGATC	3 cut(s)	93, 415, 536
AlwNI	CAGNNNCTG	1 cut(s)	259
Ama87I	CYCGRG	2 cut(s)	294, 1363
AoxI	GGCC	4 cut(s)	139, 264, 439, 780
ApaLI	GTGCAC	1 cut(s)	741
ApeKI	GCWGC	7 cut(s)	165, 305, 308, 763, 1127, 1391, 1451
ApoI	RAATTY	4 cut(s)	172, 1198, 1468, 1663
AspLEI	GCGC	4 cut(s)	137, 281, 319, 1248
AspS9I	GGNCC	2 cut(s)	439, 1339
AsuC2I	CCSGG	1 cut(s)	114
AsuHPI	GGTGA	4 cut(s)	502, 835, 1514, 1643
AvaI	CYCGRG	2 cut(s)	294, 1363
AvaII	GGWCC	1 cut(s)	1339
AxyI	CCTNAGG	1 cut(s)	1404
BaeGI	GKGCMC	3 cut(s)	72, 745, 922
BanI	GGYRCC	3 cut(s)	67, 856, 1372
BanII	GRGCYC	2 cut(s)	114, 685
BarI	GAAGNNNNNNTAC	2 cut(s)	1263, 1295
BauI	CACGAG	1 cut(s)	702
Bbv12I	GWGCWC	3 cut(s)	510, 685, 745
BbvI	GCAGC	7 cut(s)	177, 292, 295, 750, 1114, 1378, 1438
BccI	CCATC	4 cut(s)	155, 256, 332, 430
BceAI	ACGGC	1 cut(s)	172
BcgI	CGANNNNNNTGC	2 cut(s)	87, 121
BclI	TGATCA	2 cut(s)	418, 1492
BcnI	CCSGG	1 cut(s)	114
BcoDI	GTCTC	6 cut(s)	280, 322, 407, 612, 1105, 1187
BfaI	CTAG	3 cut(s)	216, 1527, 1580
BfmI	CTRYAG	1 cut(s)	1416
BfoI	RGCGCY	1 cut(s)	1249
BisI	GCNGC	8 cut(s)	166, 306, 309, 764, 1016, 1128, 1392, 1452
BlsI	GCNGC	8 cut(s)	167, 307, 310, 765, 1017, 1129, 1393, 1453
BmcAI	AGTACT	2 cut(s)	372, 1649
Bme1390I	CCNGG	1 cut(s)	114
Bme18I	GGWCC	1 cut(s)	1339
BmeT110I	CYCGRG	2 cut(s)	294, 1363
BmgT120I	GGNCC	2 cut(s)	439, 1339
BmiI	GGNNCC	3 cut(s)	69, 858, 1374
BmrFI	CCNGG	1 cut(s)	114
BmrI	ACTGGG	1 cut(s)	194
BmsI	GCATC	9 cut(s)	435, 484, 898, 1249, 1414, 1438, 1537, 1544, 1582
BmuI	ACTGGG	1 cut(s)	194
BplI	GAGNNNNNCTC	6 cut(s)	101, 133, 277, 309, 515, 547
BpmI	CTGGAG	1 cut(s)	62
Bpu10I	CCTNAGC	1 cut(s)	231
BpuEI	CTTGAG	1 cut(s)	674
BpuMI	CCSGG	1 cut(s)	114
Bsa29I	ATCGAT	1 cut(s)	588
BsaI	GGTCTC	1 cut(s)	1105
BsaJI	CCNNGG	1 cut(s)	513
BsaXI	ACNNNNNCTCC	2 cut(s)	227, 257
Bsc4I	CCNNNNNNNGG	4 cut(s)	853, 996, 997, 1405
Bse118I	RCCGGY	1 cut(s)	137
Bse1I	ACTGG	5 cut(s)	57, 189, 632, 948, 1101
Bse21I	CCTNAGG	1 cut(s)	1404
Bse3DI	GCAATG	2 cut(s)	344, 1461
BseCI	ATCGAT	1 cut(s)	588
BseDI	CCNNGG	1 cut(s)	513
BseGI	GGATG	3 cut(s)	868, 933, 1180
BseLI	CCNNNNNNNGG	4 cut(s)	853, 996, 997, 1405
BseMI	GCAATG	2 cut(s)	344, 1461
BseMII	CTCAG	5 cut(s)	245, 296, 648, 914, 1395
BseNI	ACTGG	5 cut(s)	57, 189, 632, 948, 1101
BseRI	GAGGAG	1 cut(s)	536
BseSI	GKGCMC	3 cut(s)	72, 745, 922
BseX3I	CGGCCG	1 cut(s)	139
BseXI	GCAGC	7 cut(s)	177, 292, 295, 750, 1114, 1378, 1438
BseYI	CCCAGC	1 cut(s)	846
BsgI	GTGCAG	1 cut(s)	813
Bsh1236I	CGCG	1 cut(s)	279
Bsh1285I	CGRYCG	2 cut(s)	142, 1311
BshFI	GGCC	4 cut(s)	141, 266, 441, 782
BshNI	GGYRCC	3 cut(s)	67, 856, 1372
BshVI	ATCGAT	1 cut(s)	588
BsiEI	CGRYCG	2 cut(s)	142, 1311
BsiHKAI	GWGCWC	3 cut(s)	510, 685, 745
BsiHKCI	CYCGRG	2 cut(s)	294, 1363
BsiSI	CCGG	4 cut(s)	114, 138, 860, 1012
BslFI	GGGAC	3 cut(s)	37, 1345, 1380
BslI	CCNNNNNNNGG	4 cut(s)	853, 996, 997, 1405
BsmAI	GTCTC	6 cut(s)	280, 322, 407, 612, 1105, 1187
BsmFI	GGGAC	3 cut(s)	37, 1345, 1380
BsnI	GGCC	4 cut(s)	141, 266, 441, 782
Bso31I	GGTCTC	1 cut(s)	1105
BsoBI	CYCGRG	2 cut(s)	294, 1363
Bsp1286I	GDGCHC	6 cut(s)	72, 114, 510, 685, 745, 922
Bsp143I	GATC	8 cut(s)	98, 120, 379, 407, 418, 541, 1298, 1492
Bsp19I	CCATGG	1 cut(s)	513
BspACI	CCGC	5 cut(s)	32, 424, 1015, 1307, 1670
BspANI	GGCC	4 cut(s)	141, 266, 441, 782
BspCNI	CTCAG	5 cut(s)	244, 295, 647, 915, 1396
BspDI	ATCGAT	1 cut(s)	588
BspFNI	CGCG	1 cut(s)	279
BspHI	TCATGA	1 cut(s)	432
BspLI	GGNNCC	3 cut(s)	69, 858, 1374
BspMAI	CTGCAG	1 cut(s)	1420
BspPI	GGATC	3 cut(s)	93, 415, 536
BspT107I	GGYRCC	3 cut(s)	67, 856, 1372
BspTNI	GGTCTC	1 cut(s)	1105
BsrDI	GCAATG	2 cut(s)	344, 1461
BsrFI	RCCGGY	1 cut(s)	137
BsrI	ACTGG	5 cut(s)	57, 189, 632, 948, 1101
BssAI	RCCGGY	1 cut(s)	137
BssECI	CCNNGG	1 cut(s)	513
BssMI	GATC	8 cut(s)	98, 120, 379, 407, 418, 541, 1298, 1492
BssSI	CACGAG	1 cut(s)	702
BssT1I	CCWWGG	1 cut(s)	513
Bst2BI	CACGAG	1 cut(s)	702
Bst4CI	ACNGT	3 cut(s)	67, 791, 1160
Bst6I	CTCTTC	2 cut(s)	78, 703
BstAPI	GCANNNNNTGC	3 cut(s)	1259, 1415, 1424
BstC8I	GCNNGC	6 cut(s)	110, 139, 426, 643, 741, 1125
BstDEI	CTNAG	6 cut(s)	231, 282, 614, 634, 923, 1404
BstDSI	CCRYGG	1 cut(s)	513
BstF5I	GGATG	3 cut(s)	868, 933, 1180
BstFNI	CGCG	1 cut(s)	279
BstH2I	RGCGCY	1 cut(s)	1249
BstHHI	GCGC	4 cut(s)	137, 281, 319, 1248
BstKTI	GATC	8 cut(s)	101, 123, 382, 410, 421, 544, 1301, 1495
BstMAI	GTCTC	6 cut(s)	280, 322, 407, 612, 1105, 1187
BstMBI	GATC	8 cut(s)	98, 120, 379, 407, 418, 541, 1298, 1492
BstMCI	CGRYCG	2 cut(s)	142, 1311
BstNSI	RCATGY	1 cut(s)	335
BstSCI	CCNGG	1 cut(s)	112
BstSFI	CTRYAG	1 cut(s)	1416
BstSLI	GKGCMC	3 cut(s)	72, 745, 922
BstUI	CGCG	1 cut(s)	279
BstV1I	GCAGC	7 cut(s)	177, 292, 295, 750, 1114, 1378, 1438
BstZI	CGGCCG	1 cut(s)	139
Bsu15I	ATCGAT	1 cut(s)	588
Bsu36I	CCTNAGG	1 cut(s)	1404
BsuRI	GGCC	4 cut(s)	141, 266, 441, 782
BsuTUI	ATCGAT	1 cut(s)	588
BtgI	CCRYGG	1 cut(s)	513
BtgZI	GCGATG	1 cut(s)	174
BtsCI	GGATG	3 cut(s)	868, 933, 1180
BtsIMutI	CAGTG	6 cut(s)	642, 796, 920, 955, 1165, 1599
Cac8I	GCNNGC	6 cut(s)	110, 139, 426, 643, 741, 1125
CaiI	CAGNNNCTG	1 cut(s)	259
CciI	TCATGA	1 cut(s)	432
CfoI	GCGC	4 cut(s)	137, 281, 319, 1248
Cfr10I	RCCGGY	1 cut(s)	137
Cfr13I	GGNCC	2 cut(s)	439, 1339
ClaI	ATCGAT	1 cut(s)	588
Csp6I	GTAC	5 cut(s)	371, 398, 1050, 1280, 1648
CviAII	CATG	8 cut(s)	27, 73, 332, 433, 514, 686, 889, 1285
CviQI	GTAC	5 cut(s)	371, 398, 1050, 1280, 1648
DdeI	CTNAG	6 cut(s)	231, 282, 614, 634, 923, 1404
DpnI	GATC	8 cut(s)	100, 122, 381, 409, 420, 543, 1300, 1494
DpnII	GATC	8 cut(s)	98, 120, 379, 407, 418, 541, 1298, 1492
EaeI	YGGCCR	2 cut(s)	139, 264
EagI	CGGCCG	1 cut(s)	139
Eam1104I	CTCTTC	2 cut(s)	78, 703
EarI	CTCTTC	2 cut(s)	78, 703
Ecl136II	GAGCTC	1 cut(s)	683
EclXI	CGGCCG	1 cut(s)	139
Eco130I	CCWWGG	1 cut(s)	513
Eco24I	GRGCYC	2 cut(s)	114, 685
Eco31I	GGTCTC	1 cut(s)	1105
Eco32I	GATATC	2 cut(s)	1009, 1078
Eco47I	GGWCC	1 cut(s)	1339
Eco52I	CGGCCG	1 cut(s)	139
Eco53kI	GAGCTC	1 cut(s)	683
Eco57I	CTGAAG	7 cut(s)	701, 836, 880, 1082, 1152, 1248, 1593
Eco81I	CCTNAGG	1 cut(s)	1404
Eco88I	CYCGRG	2 cut(s)	294, 1363
EcoICRI	GAGCTC	1 cut(s)	683
EcoRI	GAATTC	1 cut(s)	1663
EcoRV	GATATC	2 cut(s)	1009, 1078
EcoT14I	CCWWGG	1 cut(s)	513
EcoT22I	ATGCAT	2 cut(s)	499, 1537
EcoT38I	GRGCYC	2 cut(s)	114, 685
ErhI	CCWWGG	1 cut(s)	513
FaeI	CATG	8 cut(s)	30, 76, 335, 436, 517, 689, 892, 1288
FaqI	GGGAC	3 cut(s)	37, 1345, 1380
FatI	CATG	8 cut(s)	26, 72, 331, 432, 513, 685, 888, 1284
FauI	CCCGC	3 cut(s)	25, 417, 1663
FbaI	TGATCA	2 cut(s)	418, 1492
FblI	GTMKAC	1 cut(s)	1354
Fnu4HI	GCNGC	8 cut(s)	166, 306, 309, 764, 1016, 1128, 1392, 1452
FokI	GGATG	3 cut(s)	875, 940, 1187
FriOI	GRGCYC	2 cut(s)	114, 685
Fsp4HI	GCNGC	8 cut(s)	166, 306, 309, 764, 1016, 1128, 1392, 1452
FspBI	CTAG	3 cut(s)	216, 1527, 1580
GlaI	GCGC	4 cut(s)	136, 280, 318, 1247
GluI	GCNGC	8 cut(s)	166, 306, 309, 764, 1016, 1128, 1392, 1452
GsaI	CCCAGC	1 cut(s)	850
GsuI	CTGGAG	1 cut(s)	62
HaeII	RGCGCY	1 cut(s)	1249
HaeIII	GGCC	4 cut(s)	141, 266, 441, 782
HapII	CCGG	4 cut(s)	114, 138, 860, 1012
HhaI	GCGC	4 cut(s)	137, 281, 319, 1248
Hin1II	CATG	8 cut(s)	30, 76, 335, 436, 517, 689, 892, 1288
Hin6I	GCGC	4 cut(s)	135, 279, 317, 1246
HinP1I	GCGC	4 cut(s)	135, 279, 317, 1246
HincII	GTYRAC	2 cut(s)	822, 1355
HindII	GTYRAC	2 cut(s)	822, 1355
HindIII	AAGCTT	1 cut(s)	1314
HinfI	GANTC	7 cut(s)	208, 288, 297, 695, 711, 866, 1223
HpaII	CCGG	4 cut(s)	114, 138, 860, 1012
HphI	GGTGA	4 cut(s)	502, 835, 1514, 1643
Hpy166II	GTNNAC	5 cut(s)	743, 822, 843, 1238, 1355
Hpy188I	TCNGA	8 cut(s)	285, 384, 412, 546, 899, 1447, 1511, 1540
Hpy8I	GTNNAC	5 cut(s)	743, 822, 843, 1238, 1355
Hpy99I	CGWCG	1 cut(s)	1356
HpyCH4III	ACNGT	3 cut(s)	67, 791, 1160
HpyF3I	CTNAG	6 cut(s)	231, 282, 614, 634, 923, 1404
Hsp92II	CATG	8 cut(s)	30, 76, 335, 436, 517, 689, 892, 1288
HspAI	GCGC	4 cut(s)	135, 279, 317, 1246
KroI	GCCGGC	1 cut(s)	137
KroNI	GCCGGC	1 cut(s)	139
Ksp22I	TGATCA	2 cut(s)	418, 1492
Kzo9I	GATC	8 cut(s)	98, 120, 379, 407, 418, 541, 1298, 1492
LmnI	GCTCC	5 cut(s)	256, 453, 760, 1033, 1120
Lsp1109I	GCAGC	7 cut(s)	177, 292, 295, 750, 1114, 1378, 1438
LweI	GCATC	9 cut(s)	435, 484, 898, 1249, 1414, 1438, 1537, 1544, 1582
MaeI	CTAG	3 cut(s)	216, 1527, 1580
MaeIII	GTNAC	1 cut(s)	1618
MalI	GATC	8 cut(s)	100, 122, 381, 409, 420, 543, 1300, 1494
MboI	GATC	8 cut(s)	98, 120, 379, 407, 418, 541, 1298, 1492
MboII	GAAGA	9 cut(s)	95, 368, 389, 690, 1013, 1075, 1155, 1194, 1586
MfeI	CAATTG	3 cut(s)	58, 351, 467
MhlI	GDGCHC	6 cut(s)	72, 114, 510, 685, 745, 922
MlyI	GAGTC	4 cut(s)	282, 306, 689, 705
MmeI	TCCRAC	2 cut(s)	126, 1489
MnlI	CCTC	8 cut(s)	37, 79, 229, 514, 517, 918, 1322, 1399
Mph1103I	ATGCAT	2 cut(s)	499, 1537
MroNI	GCCGGC	1 cut(s)	137
MseI	TTAA	4 cut(s)	192, 474, 801, 1029
MslI	CAYNNNNRTG	2 cut(s)	336, 456
MspA1I	CMGCKG	2 cut(s)	424, 1670
MspI	CCGG	4 cut(s)	114, 138, 860, 1012
MspR9I	CCNGG	1 cut(s)	114
MunI	CAATTG	3 cut(s)	58, 351, 467
MvnI	CGCG	1 cut(s)	279
NaeI	GCCGGC	1 cut(s)	139
NciI	CCSGG	1 cut(s)	114
NcoI	CCATGG	1 cut(s)	513
NdeII	GATC	8 cut(s)	98, 120, 379, 407, 418, 541, 1298, 1492
NgoMIV	GCCGGC	1 cut(s)	137
NlaIII	CATG	8 cut(s)	30, 76, 335, 436, 517, 689, 892, 1288
NlaIV	GGNNCC	3 cut(s)	69, 858, 1374
NmeAIII	GCCGAG	1 cut(s)	167
NsiI	ATGCAT	2 cut(s)	499, 1537
NspI	RCATGY	1 cut(s)	335
PaeR7I	CTCGAG	1 cut(s)	294
PagI	TCATGA	1 cut(s)	432
PdiI	GCCGGC	1 cut(s)	139
PfeI	GAWTC	3 cut(s)	208, 866, 1223
PkrI	GCNGC	8 cut(s)	167, 307, 310, 765, 1017, 1129, 1393, 1453
PleI	GAGTC	4 cut(s)	282, 305, 689, 705
PpsI	GAGTC	4 cut(s)	282, 305, 689, 705
Psp124BI	GAGCTC	1 cut(s)	685
PspFI	CCCAGC	1 cut(s)	846
PspN4I	GGNNCC	3 cut(s)	69, 858, 1374
PspPI	GGNCC	2 cut(s)	439, 1339
PspXI	VCTCGAGB	1 cut(s)	294
PstI	CTGCAG	1 cut(s)	1420
PstNI	CAGNNNCTG	1 cut(s)	259
RsaI	GTAC	5 cut(s)	372, 399, 1051, 1281, 1649
RsaNI	GTAC	5 cut(s)	371, 398, 1050, 1280, 1648
RseI	CAYNNNNRTG	2 cut(s)	336, 456
SacI	GAGCTC	1 cut(s)	685
SalI	GTCGAC	1 cut(s)	1353
SaqAI	TTAA	4 cut(s)	192, 474, 801, 1029
SatI	GCNGC	8 cut(s)	166, 306, 309, 764, 1016, 1128, 1392, 1452
Sau3AI	GATC	8 cut(s)	98, 120, 379, 407, 418, 541, 1298, 1492
Sau96I	GGNCC	2 cut(s)	439, 1339
ScaI	AGTACT	2 cut(s)	372, 1649
SchI	GAGTC	4 cut(s)	282, 306, 689, 705
ScrFI	CCNGG	1 cut(s)	114
SduI	GDGCHC	6 cut(s)	72, 114, 510, 685, 745, 922
SfaNI	GCATC	9 cut(s)	435, 484, 898, 1249, 1414, 1438, 1537, 1544, 1582
SfcI	CTRYAG	1 cut(s)	1416
Sfr274I	CTCGAG	1 cut(s)	294
SinI	GGWCC	1 cut(s)	1339
SlaI	CTCGAG	1 cut(s)	294
SmiMI	CAYNNNNRTG	2 cut(s)	336, 456
SmlI	CTYRAG	2 cut(s)	294, 653
SmoI	CTYRAG	2 cut(s)	294, 653
SsiI	CCGC	5 cut(s)	32, 424, 1015, 1307, 1670
SspMI	CTAG	3 cut(s)	216, 1527, 1580
SstI	GAGCTC	1 cut(s)	685
StyD4I	CCNGG	1 cut(s)	112
StyI	CCWWGG	1 cut(s)	513
TaaI	ACNGT	3 cut(s)	67, 791, 1160
TaqI	TCGA	9 cut(s)	48, 119, 295, 588, 1080, 1311, 1354, 1422, 1614
TatI	WGTACW	3 cut(s)	370, 397, 1647
TauI	GCSGC	1 cut(s)	1018
TfiI	GAWTC	3 cut(s)	208, 866, 1223
Tru1I	TTAA	4 cut(s)	192, 474, 801, 1029
Tru9I	TTAA	4 cut(s)	192, 474, 801, 1029
TscAI	CASTG	6 cut(s)	642, 796, 927, 955, 1165, 1606
TseI	GCWGC	7 cut(s)	165, 305, 308, 763, 1127, 1391, 1451
TspDTI	ATGAA	4 cut(s)	333, 827, 879, 1038
TspGWI	ACGGA	1 cut(s)	1340
TspRI	CASTG	6 cut(s)	642, 796, 927, 955, 1165, 1606
VneI	GTGCAC	1 cut(s)	741
VpaK11BI	GGWCC	1 cut(s)	1339
XapI	RAATTY	4 cut(s)	172, 1198, 1468, 1663
XceI	RCATGY	1 cut(s)	335
XhoI	CTCGAG	1 cut(s)	294
XmiI	GTMKAC	1 cut(s)	1354
XspI	CTAG	3 cut(s)	216, 1527, 1580
ZrmI	AGTACT	2 cut(s)	372, 1649
Zsp2I	ATGCAT	2 cut(s)	499, 1537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.