MD15G1173200.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008134
Physical Location & Seq
Forward (+)
13518475 .. 13525235
6761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1173200.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGACGTAACCAACAGGTACATTACCATAAAATCACCCCTCGACGACGAAGAACCGAAAGAGTCAGACTTCGAGCTCAAAACTTCACCCCTTGCTCTATCAGTTTCCCCTGGTTCCAGTGATGTCATCGTGAAAAATCTCTATGTGTCAATCGATCCGTACCAGCTCAACCGCATGAAAAGTTTCAGCTCCTCCCACAAAGCCATAAGCTATGCAGCTGCCATCGCCCCAGGAGATGAAATTGATGCTTACGGTGTGGCAAAAGTTGTGGCTTCTGGGAATCCAGAGTTTGAGAAGGATGACTTGGTTGTGGGGCTCATCAGTTGGGGAGAGTACACTGTTCTAAAAGAAGGGACCATGTTTAGGAAATTTGACCCTTTGGGATTTCCACTTTCTTATCAAGTTGGAATTCTAGGTTTTAGTGGACTTACAGCATATGGGGGATTTTTTGAAGTGTGCAAGCCTAAAAAGGGGGAGAGAGTTTTCGTATCTGCAGCTTCTGGATCGGTTGGAAATTTGGTCGGACAGTATGCAAAACTCTTCGGTTGCTATGTTGTTGGCTGTGCCGGAAGCAAAGAAAAGGTGGCATTGCTCAAGGCAAAGCTCGGATTTGATGATGCCTTCAACTACAAGGAAGAGCCCGATTTAAAGTCGACTCTCAAAAAGTACTTCCCGGATGGGATTGATATATATTTTGACAATGTGGGATCTGAGATGCTGGAAGCAGCAGTTGAGAACATGAACACCTTCGGTAGGGTTGCTGTCTGCGGCGTGATTTCTGAGTACATAGATGCCAGAAAGCGAGCTGCGCCAAATATGCTAGACGTTGTTTACAAGAGAATTAAGATCCAAGGGTTTTTAGCAGCTGATCTCATGAATGTGTACTCTGATTTTATCTCCACCACCGTTGATCATCTTCGCACGGGCAATTTGCACGTGGTTGAAGACATCTCGAGTGGTTTGGAGAGTTTTCCATCTGCTTTCATTGGACTTTTTCGCGGTCATAACACCGGAAAGAAAATGGTTAAGCTTGCAGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

348

Amino Acids

37.9

Weight (kDa)

5.34

Isoelectric Point (pI)

21.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 17 - 120 2.4e-17 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 170 - 295 2.3e-23 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 202 - 339 3.5e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 653
AccII CGCG 1 cut(s) 999
AciI CCGC 3 cut(s) 172, 768, 999
AclWI GGATC 4 cut(s) 149, 511, 715, 841
AcsI RAATTY 3 cut(s) 368, 408, 514
AcvI CACGTG 1 cut(s) 937
AfaI GTAC 6 cut(s) 20, 161, 335, 668, 785, 884
AfiI CCNNNNNNNGG 3 cut(s) 363, 470, 753
AgsI TTSAA 3 cut(s) 452, 625, 944
AjnI CCWGG 2 cut(s) 109, 229
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
Alw21I GWGCWC 1 cut(s) 78
AlwI GGATC 4 cut(s) 149, 511, 715, 841
AlwNI CAGNNNCTG 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 952
ApeKI GCWGC 6 cut(s) 215, 218, 494, 725, 806, 863
ApoI RAATTY 3 cut(s) 368, 408, 514
Asp700I GAANNNNTTC 2 cut(s) 182, 746
AspLEI GCGC 1 cut(s) 811
AspS9I GGNCC 1 cut(s) 354
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 2 cut(s) 27, 78
AvaI CYCGRG 1 cut(s) 952
AvaII GGWCC 1 cut(s) 354
BanII GRGCYC 3 cut(s) 78, 318, 642
BbrPI CACGTG 1 cut(s) 937
BbsI GAAGAC 1 cut(s) 951
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 6 cut(s) 205, 227, 506, 737, 793, 875
BccI CCATC 3 cut(s) 230, 671, 982
BciT130I CCWGG 2 cut(s) 111, 231
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 1 cut(s) 674
BfaI CTAG 2 cut(s) 413, 821
BfmI CTRYAG 1 cut(s) 492
BisI GCNGC 7 cut(s) 216, 219, 495, 726, 769, 807, 864
BlsI GCNGC 7 cut(s) 217, 220, 496, 727, 770, 808, 865
BmcAI AGTACT 1 cut(s) 668
Bme1390I CCNGG 3 cut(s) 111, 231, 674
Bme18I GGWCC 1 cut(s) 354
BmeT110I CYCGRG 1 cut(s) 952
BmgT120I GGNCC 1 cut(s) 354
BmiI GGNNCC 2 cut(s) 115, 355
BmrFI CCNGG 3 cut(s) 111, 231, 674
BmsI GCATC 4 cut(s) 235, 607, 705, 781
BpiI GAAGAC 1 cut(s) 951
BpuEI CTTGAG 1 cut(s) 578
BpuMI CCSGG 1 cut(s) 674
Bsa29I ATCGAT 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 937
BsaJI CCNNGG 3 cut(s) 109, 229, 850
BsaWI WCCGGW 1 cut(s) 1010
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 470, 753
Bse1I ACTGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 587
BseBI CCWGG 2 cut(s) 111, 231
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 3 cut(s) 109, 229, 850
BseGI GGATG 2 cut(s) 304, 682
BseLI CCNNNNNNNGG 3 cut(s) 363, 470, 753
BseMI GCAATG 1 cut(s) 587
BseMII CTCAG 2 cut(s) 702, 771
BseNI ACTGG 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 181
BseXI GCAGC 6 cut(s) 205, 227, 506, 737, 793, 875
Bsh1236I CGCG 1 cut(s) 999
BshVI ATCGAT 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 952
BsiSI CCGG 3 cut(s) 567, 674, 1011
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 363, 470, 753
BsmFI GGGAC 1 cut(s) 367
BsoBI CYCGRG 1 cut(s) 952
Bsp1286I GDGCHC 3 cut(s) 78, 318, 642
Bsp143I GATC 6 cut(s) 154, 503, 707, 846, 868, 910
BspACI CCGC 3 cut(s) 172, 768, 999
BspCNI CTCAG 2 cut(s) 703, 772
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 1 cut(s) 999
BspHI TCATGA 1 cut(s) 873
BspLI GGNNCC 2 cut(s) 115, 355
BspMAI CTGCAG 1 cut(s) 496
BspPI GGATC 4 cut(s) 149, 511, 715, 841
BspQI GCTCTTC 1 cut(s) 630
BsrDI GCAATG 1 cut(s) 587
BsrI ACTGG 1 cut(s) 117
BssECI CCNNGG 3 cut(s) 109, 229, 850
BssMI GATC 6 cut(s) 154, 503, 707, 846, 868, 910
BssT1I CCWWGG 1 cut(s) 850
Bst2UI CCWGG 2 cut(s) 111, 231
Bst4CI ACNGT 4 cut(s) 254, 340, 528, 907
Bst6I CTCTTC 2 cut(s) 545, 630
BstBAI YACGTR 1 cut(s) 937
BstC8I GCNNGC 3 cut(s) 461, 804, 1032
BstDEI CTNAG 2 cut(s) 711, 780
BstENI CCTNNNNNAGG 1 cut(s) 751
BstF5I GGATG 2 cut(s) 304, 682
BstFNI CGCG 1 cut(s) 999
BstHHI GCGC 1 cut(s) 811
BstKTI GATC 6 cut(s) 157, 506, 710, 849, 871, 913
BstMBI GATC 6 cut(s) 154, 503, 707, 846, 868, 910
BstMWI GCNNNNNNNGC 3 cut(s) 224, 808, 817
BstNI CCWGG 2 cut(s) 111, 231
BstSCI CCNGG 3 cut(s) 109, 229, 672
BstSFI CTRYAG 1 cut(s) 492
BstUI CGCG 1 cut(s) 999
BstV1I GCAGC 6 cut(s) 205, 227, 506, 737, 793, 875
BstV2I GAAGAC 1 cut(s) 951
BstX2I RGATCY 2 cut(s) 707, 846
BstYI RGATCY 2 cut(s) 707, 846
Bsu15I ATCGAT 1 cut(s) 153
BsuTUI ATCGAT 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 208
BtsCI GGATG 2 cut(s) 304, 682
BtsIMutI CAGTG 2 cut(s) 124, 336
Cac8I GCNNGC 3 cut(s) 461, 804, 1032
CaiI CAGNNNCTG 1 cut(s) 500
CciI TCATGA 1 cut(s) 873
CfoI GCGC 1 cut(s) 811
Cfr13I GGNCC 1 cut(s) 354
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 6 cut(s) 19, 160, 334, 667, 784, 883
CspCI CAANNNNNGTGG 4 cut(s) 249, 284, 889, 924
CviAII CATG 4 cut(s) 175, 358, 739, 874
CviQI GTAC 6 cut(s) 19, 160, 334, 667, 784, 883
DdeI CTNAG 2 cut(s) 711, 780
DpnI GATC 6 cut(s) 156, 505, 709, 848, 870, 912
DpnII GATC 6 cut(s) 154, 503, 707, 846, 868, 910
DraI TTTAAA 1 cut(s) 648
Eam1104I CTCTTC 2 cut(s) 545, 630
EarI CTCTTC 2 cut(s) 545, 630
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 850
Eco24I GRGCYC 3 cut(s) 78, 318, 642
Eco47I GGWCC 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 76
Eco72I CACGTG 1 cut(s) 937
Eco88I CYCGRG 1 cut(s) 952
EcoICRI GAGCTC 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 751
EcoRI GAATTC 1 cut(s) 408
EcoRII CCWGG 2 cut(s) 109, 229
EcoT14I CCWWGG 1 cut(s) 850
EcoT38I GRGCYC 3 cut(s) 78, 318, 642
ErhI CCWWGG 1 cut(s) 850
FaeI CATG 4 cut(s) 178, 361, 742, 877
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 367
FatI CATG 4 cut(s) 174, 357, 738, 873
FauNDI CATATG 1 cut(s) 436
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 1 cut(s) 653
Fnu4HI GCNGC 7 cut(s) 216, 219, 495, 726, 769, 807, 864
FokI GGATG 2 cut(s) 311, 689
FriOI GRGCYC 3 cut(s) 78, 318, 642
Fsp4HI GCNGC 7 cut(s) 216, 219, 495, 726, 769, 807, 864
FspBI CTAG 2 cut(s) 413, 821
GlaI GCGC 1 cut(s) 810
GluI GCNGC 7 cut(s) 216, 219, 495, 726, 769, 807, 864
HapII CCGG 3 cut(s) 567, 674, 1011
HhaI GCGC 1 cut(s) 811
Hin1II CATG 4 cut(s) 178, 361, 742, 877
Hin6I GCGC 1 cut(s) 809
HinP1I GCGC 1 cut(s) 809
HincII GTYRAC 1 cut(s) 654
HindII GTYRAC 1 cut(s) 654
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 3 cut(s) 62, 280, 655
HpaII CCGG 3 cut(s) 567, 674, 1011
HphI GGTGA 2 cut(s) 27, 78
Hpy166II GTNNAC 5 cut(s) 336, 425, 654, 832, 883
Hpy188I TCNGA 6 cut(s) 67, 524, 608, 712, 781, 889
Hpy188III TCNNGA 5 cut(s) 130, 284, 501, 874, 952
Hpy8I GTNNAC 5 cut(s) 336, 425, 654, 832, 883
Hpy99I CGWCG 2 cut(s) 47, 50
HpyAV CCTTC 4 cut(s) 289, 344, 631, 757
HpyCH4III ACNGT 4 cut(s) 254, 340, 528, 907
HpyCH4IV ACGT 3 cut(s) 6, 825, 936
HpyCH4V TGCA 6 cut(s) 215, 459, 494, 533, 934, 1034
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 808, 817
HpyF3I CTNAG 2 cut(s) 711, 780
HpySE526I ACGT 3 cut(s) 6, 825, 936
Hsp92II CATG 4 cut(s) 178, 361, 742, 877
HspAI GCGC 1 cut(s) 809
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 6 cut(s) 154, 503, 707, 846, 868, 910
LguI GCTCTTC 1 cut(s) 630
LmnI GCTCC 1 cut(s) 194
Lsp1109I GCAGC 6 cut(s) 205, 227, 506, 737, 793, 875
LweI GCATC 4 cut(s) 235, 607, 705, 781
MaeI CTAG 2 cut(s) 413, 821
MaeII ACGT 3 cut(s) 6, 825, 936
MaeIII GTNAC 1 cut(s) 7
MalI GATC 6 cut(s) 156, 505, 709, 848, 870, 912
MboI GATC 6 cut(s) 154, 503, 707, 846, 868, 910
MboII GAAGA 5 cut(s) 62, 532, 647, 908, 956
MflI RGATCY 2 cut(s) 707, 846
MhlI GDGCHC 3 cut(s) 78, 318, 642
MluCI AATT 6 cut(s) 240, 368, 408, 514, 840, 928
MlyI GAGTC 2 cut(s) 71, 649
MmeI TCCRAC 3 cut(s) 385, 490, 502
MnlI CCTC 2 cut(s) 50, 202
MroXI GAANNNNTTC 2 cut(s) 182, 746
MseI TTAA 3 cut(s) 647, 843, 1026
MslI CAYNNNNRTG 1 cut(s) 878
MspA1I CMGCKG 2 cut(s) 218, 866
MspI CCGG 3 cut(s) 567, 674, 1011
MspR9I CCNGG 3 cut(s) 111, 231, 674
MvaI CCWGG 2 cut(s) 111, 231
MvnI CGCG 1 cut(s) 999
MwoI GCNNNNNNNGC 3 cut(s) 224, 808, 817
NciI CCSGG 1 cut(s) 674
NdeI CATATG 1 cut(s) 436
NdeII GATC 6 cut(s) 154, 503, 707, 846, 868, 910
NlaIII CATG 4 cut(s) 178, 361, 742, 877
NlaIV GGNNCC 2 cut(s) 115, 355
PaeR7I CTCGAG 1 cut(s) 952
PagI TCATGA 1 cut(s) 873
PciSI GCTCTTC 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 182, 746
PfeI GAWTC 1 cut(s) 280
PfoI TCCNGGA 1 cut(s) 672
PkrI GCNGC 7 cut(s) 217, 220, 496, 727, 770, 808, 865
PleI GAGTC 2 cut(s) 70, 649
PmaCI CACGTG 1 cut(s) 937
PmlI CACGTG 1 cut(s) 937
PpsI GAGTC 2 cut(s) 70, 649
Ppu21I YACGTR 1 cut(s) 937
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 109, 229
PspCI CACGTG 1 cut(s) 937
PspGI CCWGG 2 cut(s) 109, 229
PspN4I GGNNCC 2 cut(s) 115, 355
PspPI GGNCC 1 cut(s) 354
PstI CTGCAG 1 cut(s) 496
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 2 cut(s) 707, 846
PvuII CAGCTG 2 cut(s) 218, 866
RsaI GTAC 6 cut(s) 20, 161, 335, 668, 785, 884
RsaNI GTAC 6 cut(s) 19, 160, 334, 667, 784, 883
RseI CAYNNNNRTG 1 cut(s) 878
SacI GAGCTC 1 cut(s) 78
SalI GTCGAC 1 cut(s) 652
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 3 cut(s) 647, 843, 1026
SatI GCNGC 7 cut(s) 216, 219, 495, 726, 769, 807, 864
Sau3AI GATC 6 cut(s) 154, 503, 707, 846, 868, 910
Sau96I GGNCC 1 cut(s) 354
ScaI AGTACT 1 cut(s) 668
SchI GAGTC 2 cut(s) 71, 649
ScrFI CCNGG 3 cut(s) 111, 231, 674
SduI GDGCHC 3 cut(s) 78, 318, 642
SfaNI GCATC 4 cut(s) 235, 607, 705, 781
SfcI CTRYAG 1 cut(s) 492
Sfr274I CTCGAG 1 cut(s) 952
SinI GGWCC 1 cut(s) 354
SlaI CTCGAG 1 cut(s) 952
SmiMI CAYNNNNRTG 1 cut(s) 878
SmlI CTYRAG 2 cut(s) 593, 952
SmoI CTYRAG 2 cut(s) 593, 952
Sse9I AATT 6 cut(s) 240, 368, 408, 514, 840, 928
SsiI CCGC 3 cut(s) 172, 768, 999
SspMI CTAG 2 cut(s) 413, 821
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 3 cut(s) 109, 229, 672
StyI CCWWGG 1 cut(s) 850
TaaI ACNGT 4 cut(s) 254, 340, 528, 907
TaiI ACGT 3 cut(s) 9, 828, 939
TaqI TCGA 5 cut(s) 42, 72, 153, 653, 953
TasI AATT 6 cut(s) 240, 368, 408, 514, 840, 928
TatI WGTACW 4 cut(s) 333, 666, 783, 882
TauI GCSGC 1 cut(s) 771
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 3 cut(s) 647, 843, 1026
Tru9I TTAA 3 cut(s) 647, 843, 1026
TscAI CASTG 2 cut(s) 124, 343
TseI GCWGC 6 cut(s) 215, 218, 494, 725, 806, 863
TspDTI ATGAA 5 cut(s) 191, 252, 755, 890, 973
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 2 cut(s) 124, 343
VpaK11BI GGWCC 1 cut(s) 354
XagI CCTNNNNNAGG 1 cut(s) 751
XapI RAATTY 3 cut(s) 368, 408, 514
XhoI CTCGAG 1 cut(s) 952
XmiI GTMKAC 1 cut(s) 653
XmnI GAANNNNTTC 2 cut(s) 182, 746
XspI CTAG 2 cut(s) 413, 821
ZrmI AGTACT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.