Rorug01G0485000

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008134
Physical Location & Seq
Forward (+)
57187732 .. 57192422
4691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0485000.1

Sequence Viewer

Length: 3234 bp
ATGGAGTCGCTCCATTTCAGCAGCTCAAACTACTCCTCTTTTCTCTGTACCACTATTCCACTGACCACTTGTTCTCCCTTGAGCAGCTCCTCAAGAAAACCCAAGATTCGCGTCCGCATCCGAATCCGATCCTCAGTGACCCCAGACCCGTGGTCTCTCAGCGACGGAAACCCGGACCGGCCCAAGCCCAAGTCCAAGCACCCAAAGAACCCTCTCTCCGACGACAACGCCCGCCGCATAATCAAGTCCAAGGCCCTGTACCTGAGCGCCCTCCGCCGCAACCAGGGCCCACACGCCCAGACCCCTAAGTGGATCAAGAGGACGCCGGAGCAGATGGTCCGCTATTTGCAGGACGACAGAAACGGCCACCTCTACGGCCGCCACGTCGTCGCCGCCATCAAGCGCGTGAGGTCACTCTCCGAGAAGGCCGAGGGCGAGTACGACATGAGAACCGTCATGTCGTCTTTTGTGGGGAAGCTCAGCTTCAGAGAAATGTGCGTGGTGCTTAAGGAACAAAAGGGGTGGAGGCAAGTCAGGGACTTGTTTTCCTGGATGAAATTGCAGTTATGCTACCGCCCAAGTGTGATTGTGTACACTATAGTTCTAAGGACGTATGGGCAAATTGGGAAGATCAAGCTTGCTGAGCAGACCTTCTTGGAGATGCTTGAAGCTGGTTGCGAACCAGATGAAGTTGCCTGTGGAACCATGCTCTGCACATATGCCCGATGGGGCCGCGAAAAGGCTATGCTGGCATTTTATTCAGCTGTGCAGGAGAGAGGGATTCTACTCTCTGTTGCTGTTTATAATTTCATGTTGTCCTCCTTGCAGAAGAAATCGCTCCACGAAAAAGTCGTGCAGGTTTGGAGACAGATGGTGGGGGAGGGAGTGGCGCCGAATAAGTTTACTTATACTGTAGTGATTAGCTCGCTTGTCAAAGAAGGCCTCGTTGAGGAGGCTTTCAAGTGTTTTGATGAGTGTAAGAGTGTTGGATTTGTTCCTGAGGAGGCGACTTATAGTTTGCTCATCAGTTTAAGCACCAAAAGTGGCGACTATGACCAAGCACTAAGATTGTATGAGGACATGAGATCTATGAAAATTGTTCCGAGCAACTACACCTGTGCTTCATTGCTCACTTTGTATTACAAAAAGGAGGATTATTCTAAAGCCCTTTCTCTCTTTTCAGAAATGGAGAGAGAGAAAATTGCGGCTGATGAAGTCATATATGGGTTGCTGATCAGAATATATGGAAAGCTTGGTCTTTATGAGGACGCTCGGACAACATTTAAAGAGATGGAGCAGCTGGGTCTACTTAGTGATCAGAAAACATATTTAGCAATGGCACAAGTCAATCTCAATTCAGGGGATTATGACAAAGCCTTGGAGGTCATTGAACTAATGAAGTCTAGAAACAACATTTGGCTCTCAAGGTTTGCTTATATTGTCTTATTGCAGTGTTATGTTATGAAGGAAGATCTTAGTTCTGCCGAAGTTACATTTCAGGCTCTATCCAAGACTGGACTCCCAGATGCAGGTTCTTGTAATGATATGCTCAATTTGTATATAAGACTCGACTTAATGGAAAAGGCCAAGGACTTTATTGTCCAGATAAGAAGAGACCGAGTAGAATTTGACGAGGAGCTTTGCAGAACAGTTATGAGTATGTATTGCAAGGAGGGAATGGTTGGAGATACTGAACAGTTAATAAAAGAGATCAGCTCAAGTCGATTATTCAAAGATAGTAGATTCATCCAAACAATTTGTAGGGCTATGTCTGAACACAAGGATGATGAACAACTTGAAGGTAAACTTGTGACCTTTTTCCAGCCGGATACTACTGCCCTTGGATTGGTGCTTAGTCTCTATCTTGCAAATGGCAATATGTGTAAGACAGACAAAGTTGTGGCGTTGTTGCTTGAGAATTCTGGTGGCTTATCAACTGCTAGTCAGCTCATCAGGAATATTATCAGAGATGGTGATGCATATAAAGCAGAAATTCATATCAATCAATTGCTCAAGCTTGGCTGCAGAGTGGATAATGCAACAATTTCTTCTTTGATTAATTTATATGGAAAGAAACACAAGCTGAAAAAGGCCAAAGAGATATATACAGCATTTGCAGATTCACCTCTAGCTAAAAAGTTACTATGTAACTCACTGCTTGATGCTTATTCCAAATGTGGTAAATCAGAGGAAGCATACTCACTTTACAGACGGCTTACAGAAGAAGGGCATGATCTGGATGCTGTTGCCATCAGCATAGTTGTGAATGCTTTGACTCATAGTGGAAAACATAGAGAAGCAGAGAACATCATCCGCCAAAGTCTTGAACACCGATTGGAGCTTGATACTGTGGCATACAATACTTTCATTAAGGCTATGCTGGGAGCAGGTAGGTTACATTTTGCTTCTAGTATCTATGAGTGCATGCTTTCTGAGGGGGTTACTCCATCAATTCAGACATATAACACCATGATCAGTGTTTACGGACGTGGTAGAAAGTTGGATAGGGCTGTGGAAATGTTTAATACAGCTTGTAGCTTGGGTTTGTCTCCGGATGAAAAGGCATACATGAATTTGATTAGCTACTATGGAAAAGCTGGAAAAAGGCATGAAGCTTCCATGCTATTCACCAAAATGCGTGAAAGCATAAAACCTGGAATGGTAAGTTACAATATAATGATGAATGTATATGCTACTGGAGGACTTTATCAAGAAGCTGAGAAATTATTCAAAGCCATGAAGCAAGATGGTTGGTTACCTGACTCATTCACTTACCTCTCCCTAGTTCGAGCTTACACAGAGAATTTGAAATATTCAGAAGCAGAGGAAACCATTAATTCTATGCAAGAAGACGGTGTCTATCCTTCATGTTCACATTTTTACCTCATTCTCTCTGCTTTTGCCAAAATGGGATTGATAGGGGAAGCAGAAAGAGTTTATGAGGAACTACTTGCAGCAGGTTTAAGCCCAGATGCAGCTTGTTATGGGACTATGCTGCGAGGTTACATGGACTACGGACACGTGGAAGAAGGCATCAAGTTCTATGAACAAATAAGTGAGTCTATAAAGGCAGACAGGTTTATTTTGAGTGCAGCTGTGCATTTTTACAGATCTGTAGGAAAGGAAGTAGAAGCTCAGAGTGTTTTGCATTCCATGAGTAGCATGGGAATTTCATTTCTGGAGAACCTAGAAGTTGGATCCAAGCTAAAATCTCCACCAATGTTGCAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

1077

Amino Acids

121.98

Weight (kDa)

8.35

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 193 - 241 1e-07 PPR repeat family
PPR_long PF17177 247 - 378 2e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 264 - 312 2e-09 PPR repeat family
PPR_1 PF12854 295 - 325 3.9e-06 PPR repeat
PPR PF01535 301 - 331 4.8e-06 PPR repeat
PPR_2 PF13041 305 - 343 4e-06 PPR repeat family
PPR_long PF17177 316 - 419 8.3e-10 Pentacotripeptide-repeat region of PRORP
PPR_long PF17177 382 - 495 1.4e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 716 - 758 5e-06 PPR repeat family
PPR_3 PF13812 778 - 829 1.5e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 782 - 828 1e-09 PPR repeat family
PPR_2 PF13041 816 - 856 5.2e-06 PPR repeat family
PPR_3 PF13812 847 - 897 8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 885 - 932 2.5e-11 PPR repeat family
PPR PF01535 888 - 917 7.5e-07 PPR repeat
PPR_long PF17177 904 - 1021 9.7e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 908 - 969 1e-06 Pentatricopeptide repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 804
AasI GACNNNNNNGTC 1 cut(s) 1598
Acc36I ACCTGC 4 cut(s) 847, 1520, 2378, 2948
AccB1I GGYRCC 1 cut(s) 889
AccI GTMKAC 1 cut(s) 1306
AccII CGCG 3 cut(s) 111, 405, 735
AccIII TCCGGA 1 cut(s) 2551
AclWI GGATC 4 cut(s) 123, 320, 3192, 3205
AcoI YGGCCR 2 cut(s) 364, 376
AcsI RAATTY 6 cut(s) 1625, 1918, 1992, 2572, 2803, 3168
AcuI CTGAAG 1 cut(s) 469
AcvI CACGTG 1 cut(s) 3022
AcyI GRCGYC 2 cut(s) 323, 890
AfaI GTAC 4 cut(s) 49, 260, 440, 593
AfiI CCNNNNNNNGG 6 cut(s) 283, 309, 739, 949, 1529, 1846
AflII CTTAAG 1 cut(s) 506
AflIII ACRYGT 1 cut(s) 3019
AgsI TTSAA 8 cut(s) 668, 961, 1391, 1732, 1799, 2327, 2731, 2809
AhdI GACNNNNNGTC 1 cut(s) 151
AjiI CACGTC 2 cut(s) 385, 2489
AjnI CCWGG 3 cut(s) 282, 548, 2653
AjuI GAANNNNNNNTTGG 2 cut(s) 1399, 1431
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
Alw26I GTCTC 5 cut(s) 159, 859, 1608, 1862, 2553
AlwI GGATC 4 cut(s) 123, 320, 3192, 3205
Aor13HI TCCGGA 1 cut(s) 2551
ApaI GGGCCC 1 cut(s) 290
ApeKI GCWGC 8 cut(s) 21, 84, 1297, 2022, 2954, 2975, 2995, 3092
ApoI RAATTY 6 cut(s) 1625, 1918, 1992, 2572, 2803, 3168
ArsI GACNNNNNNTTYG 2 cut(s) 1000, 1032
AseI ATTAAT 2 cut(s) 2058, 2835
Asp700I GAANNNNTTC 1 cut(s) 2726
AspLEI GCGC 3 cut(s) 269, 405, 892
AspS9I GGNCC 7 cut(s) 175, 180, 253, 286, 287, 337, 730
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 3 cut(s) 1985, 2115, 2620
AvaII GGWCC 2 cut(s) 175, 337
AxyI CCTNAGG 1 cut(s) 999
BaeGI GKGCMC 1 cut(s) 290
BamHI GGATCC 1 cut(s) 3197
BanI GGYRCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 290
BbrPI CACGTG 1 cut(s) 3022
BbsI GAAGAC 1 cut(s) 2856
BbvI GCAGC 8 cut(s) 33, 96, 1309, 2009, 2966, 2982, 2987, 3104
BccI CCATC 9 cut(s) 328, 404, 720, 865, 1285, 1964, 2258, 2455, 2741
BceAI ACGGC 3 cut(s) 379, 391, 2228
BciT130I CCWGG 3 cut(s) 284, 550, 2655
BciVI GTATCC 1 cut(s) 1822
BclI TGATCA 3 cut(s) 1233, 1315, 2472
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 5 cut(s) 159, 859, 1608, 1862, 2553
BfaI CTAG 6 cut(s) 1404, 1941, 2129, 2409, 2783, 3188
BfmI CTRYAG 4 cut(s) 597, 912, 2023, 3114
BfoI RGCGCY 2 cut(s) 270, 893
BfrI CTTAAG 1 cut(s) 506
BfuAI ACCTGC 4 cut(s) 847, 1520, 2378, 2948
BfuI GTATCC 1 cut(s) 1822
BglII AGATCT 3 cut(s) 1085, 1471, 3110
BlpI GCTNAGC 2 cut(s) 479, 642
Bme1390I CCNGG 4 cut(s) 173, 284, 550, 2655
Bme18I GGWCC 2 cut(s) 175, 337
BmeRI GACNNNNNGTC 1 cut(s) 151
BmgBI CACGTC 2 cut(s) 385, 2489
BmgT120I GGNCC 7 cut(s) 175, 180, 253, 286, 287, 337, 730
BmiI GGNNCC 5 cut(s) 288, 703, 731, 891, 3199
BmrFI CCNGG 4 cut(s) 173, 284, 550, 2655
BmsI GCATC 8 cut(s) 126, 651, 1516, 1966, 2152, 2230, 2962, 3042
BpiI GAAGAC 1 cut(s) 2856
BplI GAGNNNNNCTC 2 cut(s) 1700, 1732
BpmI CTGGAG 2 cut(s) 2718, 3200
Bpu10I CCTNAGC 1 cut(s) 263
Bpu1102I GCTNAGC 2 cut(s) 479, 642
BpuEI CTTGAG 6 cut(s) 76, 100, 1408, 1702, 1934, 1997
BpuMI CCSGG 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 3022
BsaHI GRCGYC 2 cut(s) 323, 890
BsaI GGTCTC 2 cut(s) 159, 1608
BsaJI CCNNGG 7 cut(s) 149, 249, 283, 429, 1377, 1587, 1840
BsaWI WCCGGW 1 cut(s) 2551
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 200, 230
Bsc4I CCNNNNNNNGG 6 cut(s) 283, 309, 739, 949, 1529, 1846
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 1519, 2701
Bse21I CCTNAGG 1 cut(s) 999
Bse3DI GCAATG 2 cut(s) 1124, 1341
BseAI TCCGGA 1 cut(s) 2551
BseBI CCWGG 3 cut(s) 284, 550, 2655
BseDI CCNNGG 7 cut(s) 149, 249, 283, 429, 1377, 1587, 1840
BseGI GGATG 7 cut(s) 117, 558, 1746, 1789, 2245, 2310, 2560
BseLI CCNNNNNNNGG 6 cut(s) 283, 309, 739, 949, 1529, 1846
BseMI GCAATG 2 cut(s) 1124, 1341
BseMII CTCAG 9 cut(s) 147, 172, 254, 493, 633, 990, 2424, 2709, 3149
BseNI ACTGG 2 cut(s) 1519, 2701
BseRI GAGGAG 5 cut(s) 25, 79, 965, 1016, 1649
BseSI GKGCMC 1 cut(s) 290
BseX3I CGGCCG 1 cut(s) 376
BseXI GCAGC 8 cut(s) 33, 96, 1309, 2009, 2966, 2982, 2987, 3104
BseYI CCCAGC 2 cut(s) 1300, 2380
BsgI GTGCAG 4 cut(s) 697, 788, 875, 3111
Bsh1236I CGCG 3 cut(s) 111, 405, 735
Bsh1285I CGRYCG 1 cut(s) 379
BshNI GGYRCC 1 cut(s) 889
BsiEI CGRYCG 1 cut(s) 379
BsiSI CCGG 5 cut(s) 173, 178, 326, 1826, 2552
BslFI GGGAC 2 cut(s) 551, 3001
BslI CCNNNNNNNGG 6 cut(s) 283, 309, 739, 949, 1529, 1846
BsmAI GTCTC 5 cut(s) 159, 859, 1608, 1862, 2553
BsmFI GGGAC 2 cut(s) 551, 3001
BsmI GAATGC 2 cut(s) 2272, 3148
Bso31I GGTCTC 2 cut(s) 159, 1608
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 1 cut(s) 290
Bsp13I TCCGGA 1 cut(s) 2551
Bsp1407I TGTACA 1 cut(s) 591
Bsp1720I GCTNAGC 2 cut(s) 479, 642
BspCNI CTCAG 9 cut(s) 146, 171, 255, 492, 634, 991, 2425, 2710, 3148
BspEI TCCGGA 1 cut(s) 2551
BspFNI CGCG 3 cut(s) 111, 405, 735
BspLI GGNNCC 5 cut(s) 288, 703, 731, 891, 3199
BspMAI CTGCAG 1 cut(s) 2027
BspMI ACCTGC 4 cut(s) 847, 1520, 2378, 2948
BspPI GGATC 4 cut(s) 123, 320, 3192, 3205
BspT107I GGYRCC 1 cut(s) 889
BspTI CTTAAG 1 cut(s) 506
BspTNI GGTCTC 2 cut(s) 159, 1608
BsrDI GCAATG 2 cut(s) 1124, 1341
BsrFI RCCGGY 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 2 cut(s) 1519, 2701
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 7 cut(s) 149, 249, 283, 429, 1377, 1587, 1840
BssNI GRCGYC 2 cut(s) 323, 890
BssT1I CCWWGG 4 cut(s) 249, 1377, 1587, 1840
Bst2UI CCWGG 3 cut(s) 284, 550, 2655
Bst4CI ACNGT 6 cut(s) 454, 913, 1651, 1698, 2350, 2855
Bst6I CTCTTC 1 cut(s) 1606
BstACI GRCGYC 2 cut(s) 323, 890
BstAFI CTTAAG 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 3022
BstC8I GCNNGC 5 cut(s) 232, 639, 750, 926, 2426
BstDSI CCRYGG 1 cut(s) 149
BstEII GGTNACC 1 cut(s) 2754
BstENI CCTNNNNNAGG 1 cut(s) 947
BstF5I GGATG 7 cut(s) 117, 558, 1746, 1789, 2245, 2310, 2560
BstFNI CGCG 3 cut(s) 111, 405, 735
BstH2I RGCGCY 2 cut(s) 270, 893
BstHHI GCGC 3 cut(s) 269, 405, 892
BstMAI GTCTC 5 cut(s) 159, 859, 1608, 1862, 2553
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 5 cut(s) 273, 285, 643, 749, 1985
BstNI CCWGG 3 cut(s) 284, 550, 2655
BstNSI RCATGY 1 cut(s) 2428
BstPI GGTNACC 1 cut(s) 2754
BstSCI CCNGG 4 cut(s) 171, 282, 548, 2653
BstSFI CTRYAG 4 cut(s) 597, 912, 2023, 3114
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 3 cut(s) 111, 405, 735
BstV1I GCAGC 8 cut(s) 33, 96, 1309, 2009, 2966, 2982, 2987, 3104
BstV2I GAAGAC 1 cut(s) 2856
BstX2I RGATCY 4 cut(s) 1085, 1471, 3110, 3197
BstXI CCANNNNNNTGG 1 cut(s) 150
BstYI RGATCY 4 cut(s) 1085, 1471, 3110, 3197
BstZI CGGCCG 1 cut(s) 376
Bsu36I CCTNAGG 1 cut(s) 999
BsuI GTATCC 1 cut(s) 1822
BtgI CCRYGG 1 cut(s) 149
BtrI CACGTC 2 cut(s) 385, 2489
BtsCI GGATG 7 cut(s) 117, 558, 1746, 1789, 2245, 2310, 2560
BtsI GCAGTG 2 cut(s) 1457, 2153
BtsIMutI CAGTG 5 cut(s) 59, 141, 1457, 2153, 2482
BveI ACCTGC 4 cut(s) 847, 1520, 2378, 2948
Cac8I GCNNGC 5 cut(s) 232, 639, 750, 926, 2426
CfoI GCGC 3 cut(s) 269, 405, 892
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 7 cut(s) 175, 180, 253, 286, 287, 337, 730
CpoI CGGWCCG 1 cut(s) 175
CseI GACGC 3 cut(s) 100, 331, 1277
Csp6I GTAC 4 cut(s) 48, 259, 439, 592
CspCI CAANNNNNGTGG 3 cut(s) 503, 538, 3204
CspI CGGWCCG 1 cut(s) 175
CviQI GTAC 4 cut(s) 48, 259, 439, 592
DinI GGCGCC 1 cut(s) 891
DraI TTTAAA 1 cut(s) 1285
DrdI GACNNNNNNGTC 1 cut(s) 1598
DriI GACNNNNNGTC 1 cut(s) 151
DseDI GACNNNNNNGTC 1 cut(s) 1598
EaeI YGGCCR 2 cut(s) 364, 376
EagI CGGCCG 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 1606
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 1606
EciI GGCGGA 2 cut(s) 263, 2303
EclXI CGGCCG 1 cut(s) 376
Eco130I CCWWGG 4 cut(s) 249, 1377, 1587, 1840
Eco147I AGGCCT 1 cut(s) 942
Eco24I GRGCYC 1 cut(s) 290
Eco31I GGTCTC 2 cut(s) 159, 1608
Eco47I GGWCC 2 cut(s) 175, 337
Eco52I CGGCCG 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 469
Eco72I CACGTG 1 cut(s) 3022
Eco81I CCTNAGG 1 cut(s) 999
Eco91I GGTNACC 1 cut(s) 2754
EcoNI CCTNNNNNAGG 1 cut(s) 947
EcoO109I RGGNCCY 2 cut(s) 253, 286
EcoO65I GGTNACC 1 cut(s) 2754
EcoRI GAATTC 1 cut(s) 1918
EcoRII CCWGG 3 cut(s) 282, 548, 2653
EcoT14I CCWWGG 4 cut(s) 249, 1377, 1587, 1840
EcoT22I ATGCAT 1 cut(s) 1981
EcoT38I GRGCYC 1 cut(s) 290
EgeI GGCGCC 1 cut(s) 891
EheI GGCGCC 1 cut(s) 891
ErhI CCWWGG 4 cut(s) 249, 1377, 1587, 1840
FalI AAGNNNNNCTT 6 cut(s) 467, 499, 1417, 1449, 1791, 1823
FaqI GGGAC 2 cut(s) 551, 3001
FauI CCCGC 1 cut(s) 239
FauNDI CATATG 1 cut(s) 718
FbaI TGATCA 3 cut(s) 1233, 1315, 2472
FblI GTMKAC 1 cut(s) 1306
FokI GGATG 7 cut(s) 104, 565, 1733, 1796, 2252, 2297, 2567
FriOI GRGCYC 1 cut(s) 290
FspBI CTAG 6 cut(s) 1404, 1941, 2129, 2409, 2783, 3188
GlaI GCGC 3 cut(s) 268, 404, 891
GsaI CCCAGC 2 cut(s) 1304, 2384
GsuI CTGGAG 2 cut(s) 2718, 3200
HaeII RGCGCY 2 cut(s) 270, 893
HapII CCGG 5 cut(s) 173, 178, 326, 1826, 2552
HgaI GACGC 3 cut(s) 100, 331, 1277
HhaI GCGC 3 cut(s) 269, 405, 892
Hin1I GRCGYC 2 cut(s) 323, 890
Hin6I GCGC 3 cut(s) 267, 403, 890
HinP1I GCGC 3 cut(s) 267, 403, 890
HindIII AAGCTT 4 cut(s) 635, 1250, 2015, 2613
HpaII CCGG 5 cut(s) 173, 178, 326, 1826, 2552
HphI GGTGA 3 cut(s) 1985, 2115, 2620
Hpy166II GTNNAC 7 cut(s) 592, 594, 903, 1307, 1805, 2482, 2873
Hpy8I GTNNAC 7 cut(s) 592, 594, 903, 1307, 1805, 2482, 2873
Hpy99I CGWCG 4 cut(s) 167, 224, 389, 392
HpyAV CCTTC 8 cut(s) 418, 661, 932, 1459, 1793, 2219, 2874, 3023
HpyCH4III ACNGT 6 cut(s) 454, 913, 1651, 1698, 2350, 2855
HpyCH4IV ACGT 4 cut(s) 384, 611, 2488, 3021
HpyF10VI GCNNNNNNNGC 5 cut(s) 273, 285, 643, 749, 1985
HpySE526I ACGT 4 cut(s) 384, 611, 2488, 3021
Hsp92I GRCGYC 2 cut(s) 323, 890
HspAI GCGC 3 cut(s) 267, 403, 890
KasI GGCGCC 1 cut(s) 889
Kpn2I TCCGGA 1 cut(s) 2551
Ksp22I TGATCA 3 cut(s) 1233, 1315, 2472
LmnI GCTCC 8 cut(s) 15, 92, 328, 843, 1294, 1636, 2338, 2384
Lsp1109I GCAGC 8 cut(s) 33, 96, 1309, 2009, 2966, 2982, 2987, 3104
LweI GCATC 8 cut(s) 126, 651, 1516, 1966, 2152, 2230, 2962, 3042
MaeI CTAG 6 cut(s) 1404, 1941, 2129, 2409, 2783, 3188
MaeII ACGT 4 cut(s) 384, 611, 2488, 3021
MboII GAAGA 8 cut(s) 640, 841, 1481, 1623, 2040, 2234, 2861, 3038
MfeI CAATTG 1 cut(s) 2006
MflI RGATCY 4 cut(s) 1085, 1471, 3110, 3197
MhlI GDGCHC 1 cut(s) 290
Mly113I GGCGCC 1 cut(s) 890
MlyI GAGTC 6 cut(s) 14, 1512, 1560, 2269, 2756, 3068
MmeI TCCRAC 5 cut(s) 243, 967, 1663, 2481, 3175
Mph1103I ATGCAT 1 cut(s) 1981
MroI TCCGGA 1 cut(s) 2551
MroXI GAANNNNTTC 1 cut(s) 2726
MslI CAYNNNNRTG 3 cut(s) 1782, 2261, 2633
MspA1I CMGCKG 3 cut(s) 764, 1300, 3095
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 5 cut(s) 173, 178, 326, 1826, 2552
MspR9I CCNGG 4 cut(s) 173, 284, 550, 2655
MunI CAATTG 1 cut(s) 2006
Mva1269I GAATGC 2 cut(s) 2272, 3148
MvaI CCWGG 3 cut(s) 284, 550, 2655
MvnI CGCG 3 cut(s) 111, 405, 735
MwoI GCNNNNNNNGC 5 cut(s) 273, 285, 643, 749, 1985
NarI GGCGCC 1 cut(s) 890
NciI CCSGG 1 cut(s) 173
NdeI CATATG 1 cut(s) 718
NlaIV GGNNCC 5 cut(s) 288, 703, 731, 891, 3199
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 3 cut(s) 136, 411, 1810
NsiI ATGCAT 1 cut(s) 1981
NspI RCATGY 1 cut(s) 2428
PaeI GCATGC 1 cut(s) 2428
PceI AGGCCT 1 cut(s) 942
PcsI WCGNNNNNNNCGW 2 cut(s) 381, 849
PctI GAATGC 2 cut(s) 2272, 3148
PdmI GAANNNNTTC 1 cut(s) 2726
PfeI GAWTC 5 cut(s) 106, 123, 781, 1743, 2120
PflFI GACNNNGTC 1 cut(s) 2855
PfoI TCCNGGA 1 cut(s) 548
PleI GAGTC 6 cut(s) 13, 1512, 1560, 2269, 2756, 3067
PluTI GGCGCC 1 cut(s) 893
PmaCI CACGTG 1 cut(s) 3022
PmlI CACGTG 1 cut(s) 3022
PpsI GAGTC 6 cut(s) 13, 1512, 1560, 2269, 2756, 3067
Ppu21I YACGTR 1 cut(s) 3022
PshBI ATTAAT 2 cut(s) 2058, 2835
PsiI TTATAA 1 cut(s) 804
Psp6I CCWGG 3 cut(s) 282, 548, 2653
PspCI CACGTG 1 cut(s) 3022
PspEI GGTNACC 1 cut(s) 2754
PspFI CCCAGC 2 cut(s) 1300, 2380
PspGI CCWGG 3 cut(s) 282, 548, 2653
PspN4I GGNNCC 5 cut(s) 288, 703, 731, 891, 3199
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 7 cut(s) 175, 180, 253, 286, 287, 337, 730
PstI CTGCAG 1 cut(s) 2027
PsuI RGATCY 4 cut(s) 1085, 1471, 3110, 3197
PsyI GACNNNGTC 1 cut(s) 2855
PvuII CAGCTG 3 cut(s) 764, 1300, 3095
RsaI GTAC 4 cut(s) 49, 260, 440, 593
RsaNI GTAC 4 cut(s) 48, 259, 439, 592
RseI CAYNNNNRTG 3 cut(s) 1782, 2261, 2633
Rsr2I CGGWCCG 1 cut(s) 175
RsrII CGGWCCG 1 cut(s) 175
Sau96I GGNCC 7 cut(s) 175, 180, 253, 286, 287, 337, 730
SchI GAGTC 6 cut(s) 14, 1512, 1560, 2269, 2756, 3068
ScrFI CCNGG 4 cut(s) 173, 284, 550, 2655
SduI GDGCHC 1 cut(s) 290
SfaNI GCATC 8 cut(s) 126, 651, 1516, 1966, 2152, 2230, 2962, 3042
SfcI CTRYAG 4 cut(s) 597, 912, 2023, 3114
SfoI GGCGCC 1 cut(s) 891
SinI GGWCC 2 cut(s) 175, 337
SmiMI CAYNNNNRTG 3 cut(s) 1782, 2261, 2633
SmlI CTYRAG 7 cut(s) 79, 91, 506, 1423, 1717, 1913, 2012
SmoI CTYRAG 7 cut(s) 79, 91, 506, 1423, 1717, 1913, 2012
SphI GCATGC 1 cut(s) 2428
SseBI AGGCCT 1 cut(s) 942
SspDI GGCGCC 1 cut(s) 889
SspI AATATT 2 cut(s) 1960, 2813
SspMI CTAG 6 cut(s) 1404, 1941, 2129, 2409, 2783, 3188
StuI AGGCCT 1 cut(s) 942
StyD4I CCNGG 4 cut(s) 171, 282, 548, 2653
StyI CCWWGG 4 cut(s) 249, 1377, 1587, 1840
TaaI ACNGT 6 cut(s) 454, 913, 1651, 1698, 2350, 2855
TaiI ACGT 4 cut(s) 387, 614, 2491, 3024
TaqI TCGA 3 cut(s) 1569, 1723, 2788
TaqII GACCGA 1 cut(s) 1632
TatI WGTACW 1 cut(s) 591
TauI GCSGC 6 cut(s) 237, 279, 381, 395, 735, 1208
TfiI GAWTC 5 cut(s) 106, 123, 781, 1743, 2120
TscAI CASTG 5 cut(s) 66, 141, 1457, 2160, 2482
TseFI GTSAC 3 cut(s) 136, 411, 1810
TseI GCWGC 8 cut(s) 21, 84, 1297, 2022, 2954, 2975, 2995, 3092
Tsp45I GTSAC 3 cut(s) 136, 411, 1810
TspGWI ACGGA 3 cut(s) 180, 2499, 3030
TspRI CASTG 5 cut(s) 66, 141, 1457, 2160, 2482
Tth111I GACNNNGTC 1 cut(s) 2855
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 2 cut(s) 175, 337
VspI ATTAAT 2 cut(s) 2058, 2835
XagI CCTNNNNNAGG 1 cut(s) 947
XapI RAATTY 6 cut(s) 1625, 1918, 1992, 2572, 2803, 3168
XbaI TCTAGA 1 cut(s) 1403
XceI RCATGY 1 cut(s) 2428
XcmI CCANNNNNNNNNTGG 1 cut(s) 3160
XmiI GTMKAC 1 cut(s) 1306
XmnI GAANNNNTTC 1 cut(s) 2726
XspI CTAG 6 cut(s) 1404, 1941, 2129, 2409, 2783, 3188
Zsp2I ATGCAT 1 cut(s) 1981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.