Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0008134

Physical Location & Seq

Genomic Coordinates Reverse (-)

57193091 .. 57194491

Gene Structure 1401 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0485100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1401 bp

ATGATCAAGCTCTGTAGAATCCAACTACCCAAAGCCACCACCAGAATTTCAGACCTTCTCAACCGAAGCTGCTTGACTACAACGACAACTGCTGGAAATTTGGATGTGCAGTCTCCAAGTATCCCGGACCCGGAAACACTAGCAACCCAGTTGTCAAATTGCAGCACTAGTTTATCTGAACTGAACCAAATCTATGCCCAAATTATCAGAACCCAGTTCCTACATCTCTACCCAGCCCCATTTCACTGGAACAATATCATAAGGTCATACACCAGACGCGATGCTCCTACCAAGTCGCTCTTTGTTTACGTTTCTATGTCACGCGCCGGTGTTTTACCCGACTGCTACACCATCCCCATTGTTTTGAAAACACTGTGTCAGCTTTTTGCTGTGGAGATCGGCAGGCAGCTTCATTCTGTGGCTGTGAGGATTGGGCTCGACTCAAATGAGTTCTGCGAGACTGGGTTTATTAACTTGTATTCCAAGGCGGGAGAGTTTGAGAATGCACGGAACGTGTTCGGTCAAAACCCTGAGAGAAAGCTGGGTTCTTGGAATGCCGTCATTGCGGGGCTTTCGCAAGGCGGGCGTGCTAGGGAAGCTGTAGACATGTTTATAGAGCTTAGGAAATGCGGGTTACTGCCGGATGATGTGACCATGGTTAGTGTCACGTCGGCTTGTGGTGGTCTGGGTGACTTGGGATTGGCTCTTCAGTTGCATAAGTGTGTTTATCAAGCTGAAATCGTTGCGGAGAAATCGGATGTGCTGATGTTGAATTCGCTTGTTGATATGTATGGGAAATGTGGGAGGATGGACTTGGCTCACAGGGTGTTTTCCAGGATGGAGCAACAGAATGTGTCGTCGTGGACGTCGATGATCGTGGGGTATGGGATGCACGGGCATGTGGATGAAGCTCTGGAATGCTTTCAGTGCATGAGGGAGGCGGGAGTGAGGCCGAACCACGTGACCTTTGTTGGGGTGCTCAGTGCATGTGTTCATGGGGGGACAGTGCAAGAGGGAAGATATTACTTTGAAATGATGAACAAGGATTATGGGATAAACCCTCGCTTGCAGCATTATGGATGCATGGTGGATCTTCTTGGCAGAGCGGGGTTGCTTGAGGAGGCGAGGAAAATGGTGGAAGACATGCCGATGAAAGCAAATTCGGCAGTCTGGGGCTGCCTGATGGGAGCCTGTGAGAAATATGGGAATGTGAAGATGGGGGAGTGGGTGGCTAAGCATTTGCAAGAACTAGAGCCATGGAATGATGGGGCTTTTGTGGTTTTATCTAATATCTATGCCAGTAGAGGTTTATGGAAAGAGGTTGAGAGGGTAAGACGGATTATGCACCAGAGAAAGCTTGCTAAGGTTCCTGGTTATAGCTTGGCAGGAAGTGCAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N_2 ADH_zinc_N

Protein Analysis

466

Amino Acids

51.92

Weight (kDa)

7.85

Isoelectric Point (pI)

40.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	183 - 224	2.2e-06	PPR repeat family
PPR_2	PF13041	284 - 331	2e-10	PPR repeat family
PPR	PF01535	287 - 316	3.7e-08	PPR repeat
E_motif	PF20431	401 - 460	4.2e-16	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008134)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g03870
malus_domestica	MD15G1173200.v1.1 MD15G1173400.v1.1
prunus_persica	Prupe.7G242400_v2.0.a1 Prupe.7G242400_v2.0.a1
pyrus_communis	pycom15g15510 pycom15g15530 pycom15g15540
rosa_chinensis	RchiOBHm_Chr2g0088551 RchiOBHm_Chr2g0088561
rosa_laevigata	RLG00000015962 RLG00000015965
rosa_multiflora	Rmu_co8462133.1_g000001 Rmu_sc0004733.1_g000007 Rmu_sc0041148.1_g000001 Rmu_sc0041185.1_g000001
rosa_roxburghii	Rroxscaffold_2G00152610
rosa_rugosa	Rorug01G0485000 Rorug01G0485100
rosa_samantha	Rh2AG039800 Rh2CG039900 Rh2DG039200
rosa_wichuraiana	Rw2G003110 Rw2G003130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	869
AccBSI	CCGCTC	1 cut(s)	1106
AccI	GTMKAC	1 cut(s)	603
AccII	CGCG	2 cut(s)	279, 324
AciI	CCGC	7 cut(s)	488, 566, 582, 630, 746, 941, 1106
AclWI	GGATC	1 cut(s)	1098
AcsI	RAATTY	4 cut(s)	45, 97, 772, 1159
AcuI	CTGAAG	1 cut(s)	692
AcvI	CACGTG	1 cut(s)	961
AcyI	GRCGYC	1 cut(s)	866
AdeI	CACNNNGTG	1 cut(s)	826
AfiI	CCNNNNNNNGG	2 cut(s)	130, 972
AflIII	ACRYGT	2 cut(s)	513, 606
AgsI	TTSAA	3 cut(s)	367, 772, 1031
AhlI	ACTAGT	1 cut(s)	167
AjiI	CACGTC	1 cut(s)	669
AjnI	CCWGG	2 cut(s)	833, 1369
Alw21I	GWGCWC	1 cut(s)	981
Alw26I	GTCTC	2 cut(s)	117, 452
AlwI	GGATC	1 cut(s)	1098
AoxI	GGCC	1 cut(s)	950
ApeKI	GCWGC	5 cut(s)	69, 162, 406, 1069, 1176
ApoI	RAATTY	4 cut(s)	45, 97, 772, 1159
Asp700I	GAANNNNTTC	2 cut(s)	515, 921
AspLEI	GCGC	1 cut(s)	326
AspS9I	GGNCC	1 cut(s)	127
AsuC2I	CCSGG	2 cut(s)	125, 131
AsuHPI	GGTGA	1 cut(s)	701
AvaII	GGWCC	1 cut(s)	127
BanII	GRGCYC	1 cut(s)	438
BbrPI	CACGTG	1 cut(s)	961
BbsI	GAAGAC	1 cut(s)	1146
Bbv12I	GWGCWC	1 cut(s)	981
BbvI	GCAGC	5 cut(s)	56, 174, 418, 1081, 1163
BccI	CCATC	6 cut(s)	359, 802, 832, 1177, 1210, 1259
BceAI	ACGGC	1 cut(s)	542
BciT130I	CCWGG	2 cut(s)	835, 1371
BciVI	GTATCC	1 cut(s)	131
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	2 cut(s)	125, 131
BcoDI	GTCTC	2 cut(s)	117, 452
BcuI	ACTAGT	1 cut(s)	167
BfaI	CTAG	4 cut(s)	140, 168, 591, 1250
BfmI	CTRYAG	2 cut(s)	13, 600
BfuI	GTATCC	1 cut(s)	131
BisI	GCNGC	5 cut(s)	70, 163, 407, 1070, 1177
BlpI	GCTNAGC	1 cut(s)	1233
BlsI	GCNGC	5 cut(s)	71, 164, 408, 1071, 1178
Bme1390I	CCNGG	4 cut(s)	125, 131, 835, 1371
Bme18I	GGWCC	1 cut(s)	127
BmgBI	CACGTC	1 cut(s)	669
BmgT120I	GGNCC	1 cut(s)	127
BmiI	GGNNCC	3 cut(s)	129, 1189, 1368
BmrFI	CCNGG	4 cut(s)	125, 131, 835, 1371
BmrI	ACTGGG	3 cut(s)	142, 208, 471
BmsI	GCATC	3 cut(s)	271, 879, 1070
BmuI	ACTGGG	3 cut(s)	142, 208, 471
BpiI	GAAGAC	1 cut(s)	1146
Bpu10I	CCTNAGC	2 cut(s)	620, 1362
Bpu1102I	GCTNAGC	1 cut(s)	1233
BpuEI	CTTGAG	1 cut(s)	1136
BpuMI	CCSGG	2 cut(s)	125, 131
BsaAI	YACGTR	1 cut(s)	961
BsaHI	GRCGYC	1 cut(s)	866
BsaJI	CCNNGG	3 cut(s)	483, 654, 1256
Bsc4I	CCNNNNNNNGG	2 cut(s)	130, 972
Bse118I	RCCGGY	1 cut(s)	326
Bse1I	ACTGG	5 cut(s)	148, 214, 251, 466, 1299
Bse3DI	GCAATG	1 cut(s)	561
BseBI	CCWGG	2 cut(s)	835, 1371
BseDI	CCNNGG	3 cut(s)	483, 654, 1256
BseGI	GGATG	9 cut(s)	109, 351, 649, 763, 813, 843, 894, 910, 1085
BseLI	CCNNNNNNNGG	2 cut(s)	130, 972
BseMI	GCAATG	1 cut(s)	561
BseMII	CTCAG	2 cut(s)	522, 994
BseNI	ACTGG	5 cut(s)	148, 214, 251, 466, 1299
BseRI	GAGGAG	1 cut(s)	1133
BseXI	GCAGC	5 cut(s)	56, 174, 418, 1081, 1163
BseYI	CCCAGC	2 cut(s)	232, 541
BsgI	GTGCAG	1 cut(s)	128
Bsh1236I	CGCG	2 cut(s)	279, 324
BshFI	GGCC	1 cut(s)	952
BsiHKAI	GWGCWC	1 cut(s)	981
BsiSI	CCGG	4 cut(s)	125, 131, 327, 641
BslFI	GGGAC	1 cut(s)	1015
BslI	CCNNNNNNNGG	2 cut(s)	130, 972
BsmAI	GTCTC	2 cut(s)	117, 452
BsmFI	GGGAC	1 cut(s)	1015
BsmI	GAATGC	3 cut(s)	508, 559, 923
BsnI	GGCC	1 cut(s)	952
Bsp1286I	GDGCHC	2 cut(s)	438, 981
Bsp143I	GATC	4 cut(s)	3, 396, 873, 1090
Bsp1720I	GCTNAGC	1 cut(s)	1233
Bsp19I	CCATGG	2 cut(s)	654, 1256
BspACI	CCGC	7 cut(s)	488, 566, 582, 630, 746, 941, 1106
BspANI	GGCC	1 cut(s)	952
BspCNI	CTCAG	2 cut(s)	523, 993
BspFNI	CGCG	2 cut(s)	279, 324
BspLI	GGNNCC	3 cut(s)	129, 1189, 1368
BspPI	GGATC	1 cut(s)	1098
BspQI	GCTCTTC	1 cut(s)	711
BsrBI	CCGCTC	1 cut(s)	1106
BsrDI	GCAATG	1 cut(s)	561
BsrFI	RCCGGY	1 cut(s)	326
BsrI	ACTGG	5 cut(s)	148, 214, 251, 466, 1299
BssAI	RCCGGY	1 cut(s)	326
BssECI	CCNNGG	3 cut(s)	483, 654, 1256
BssMI	GATC	4 cut(s)	3, 396, 873, 1090
BssNI	GRCGYC	1 cut(s)	866
BssT1I	CCWWGG	3 cut(s)	483, 654, 1256
Bst2UI	CCWGG	2 cut(s)	835, 1371
Bst4CI	ACNGT	2 cut(s)	375, 1006
Bst6I	CTCTTC	1 cut(s)	711
BstACI	GRCGYC	1 cut(s)	866
BstAPI	GCANNNNNTGC	1 cut(s)	1391
BstBAI	YACGTR	1 cut(s)	961
BstC8I	GCNNGC	5 cut(s)	404, 584, 588, 1067, 1359
BstDEI	CTNAG	5 cut(s)	531, 620, 980, 1233, 1362
BstDSI	CCRYGG	2 cut(s)	654, 1256
BstF5I	GGATG	9 cut(s)	109, 351, 649, 763, 813, 843, 894, 910, 1085
BstFNI	CGCG	2 cut(s)	279, 324
BstHHI	GCGC	1 cut(s)	326
BstKTI	GATC	4 cut(s)	6, 399, 876, 1093
BstMAI	GTCTC	2 cut(s)	117, 452
BstMBI	GATC	4 cut(s)	3, 396, 873, 1090
BstMWI	GCNNNNNNNGC	6 cut(s)	563, 583, 596, 927, 1163, 1391
BstNI	CCWGG	2 cut(s)	835, 1371
BstNSI	RCATGY	4 cut(s)	610, 902, 990, 1147
BstSCI	CCNGG	4 cut(s)	123, 129, 833, 1369
BstSFI	CTRYAG	2 cut(s)	13, 600
BstUI	CGCG	2 cut(s)	279, 324
BstV1I	GCAGC	5 cut(s)	56, 174, 418, 1081, 1163
BstV2I	GAAGAC	1 cut(s)	1146
BstX2I	RGATCY	1 cut(s)	1090
BstXI	CCANNNNNNTGG	1 cut(s)	246
BstYI	RGATCY	1 cut(s)	1090
BsuI	GTATCC	1 cut(s)	131
BsuRI	GGCC	1 cut(s)	952
BtgI	CCRYGG	2 cut(s)	654, 1256
BtgZI	GCGATG	1 cut(s)	294
BtrI	CACGTC	1 cut(s)	669
BtsCI	GGATG	9 cut(s)	109, 351, 649, 763, 813, 843, 894, 910, 1085
BtsIMutI	CAGTG	5 cut(s)	244, 371, 932, 988, 1011
Cac8I	GCNNGC	5 cut(s)	404, 584, 588, 1067, 1359
CfoI	GCGC	1 cut(s)	326
Cfr10I	RCCGGY	1 cut(s)	326
Cfr13I	GGNCC	1 cut(s)	127
CseI	GACGC	1 cut(s)	285
CviAII	CATG	9 cut(s)	607, 655, 899, 931, 987, 995, 1084, 1144, 1257
DdeI	CTNAG	5 cut(s)	531, 620, 980, 1233, 1362
DpnI	GATC	4 cut(s)	5, 398, 875, 1092
DpnII	GATC	4 cut(s)	3, 396, 873, 1090
DraIII	CACNNNGTG	1 cut(s)	826
Eam1104I	CTCTTC	1 cut(s)	711
EarI	CTCTTC	1 cut(s)	711
Eco130I	CCWWGG	3 cut(s)	483, 654, 1256
Eco24I	GRGCYC	1 cut(s)	438
Eco47I	GGWCC	1 cut(s)	127
Eco57I	CTGAAG	1 cut(s)	692
Eco72I	CACGTG	1 cut(s)	961
EcoRI	GAATTC	1 cut(s)	772
EcoRII	CCWGG	2 cut(s)	833, 1369
EcoT14I	CCWWGG	3 cut(s)	483, 654, 1256
EcoT22I	ATGCAT	1 cut(s)	1085
EcoT38I	GRGCYC	1 cut(s)	438
ErhI	CCWWGG	3 cut(s)	483, 654, 1256
FaeI	CATG	9 cut(s)	610, 658, 902, 934, 990, 998, 1087, 1147, 1260
FalI	AAGNNNNNCTT	2 cut(s)	284, 316
FaqI	GGGAC	1 cut(s)	1015
FatI	CATG	9 cut(s)	606, 654, 898, 930, 986, 994, 1083, 1143, 1256
FauI	CCCGC	6 cut(s)	481, 559, 575, 623, 934, 1099
FbaI	TGATCA	1 cut(s)	3
FblI	GTMKAC	1 cut(s)	603
Fnu4HI	GCNGC	5 cut(s)	70, 163, 407, 1070, 1177
FokI	GGATG	9 cut(s)	116, 338, 656, 770, 820, 850, 901, 917, 1092
FriOI	GRGCYC	1 cut(s)	438
Fsp4HI	GCNGC	5 cut(s)	70, 163, 407, 1070, 1177
FspBI	CTAG	4 cut(s)	140, 168, 591, 1250
GlaI	GCGC	1 cut(s)	325
GluI	GCNGC	5 cut(s)	70, 163, 407, 1070, 1177
GsaI	CCCAGC	2 cut(s)	236, 545
HaeIII	GGCC	1 cut(s)	952
HapII	CCGG	4 cut(s)	125, 131, 327, 641
HgaI	GACGC	1 cut(s)	285
HhaI	GCGC	1 cut(s)	326
Hin1I	GRCGYC	1 cut(s)	866
Hin1II	CATG	9 cut(s)	610, 658, 902, 934, 990, 998, 1087, 1147, 1260
Hin6I	GCGC	1 cut(s)	324
HinP1I	GCGC	1 cut(s)	324
HindIII	AAGCTT	1 cut(s)	1355
HinfI	GANTC	2 cut(s)	18, 440
HpaII	CCGG	4 cut(s)	125, 131, 327, 641
HphI	GGTGA	1 cut(s)	701
Hpy166II	GTNNAC	3 cut(s)	307, 604, 864
Hpy188I	TCNGA	4 cut(s)	52, 178, 209, 757
Hpy188III	TCNNGA	1 cut(s)	914
Hpy8I	GTNNAC	3 cut(s)	307, 604, 864
Hpy99I	CGWCG	3 cut(s)	673, 862, 871
HpyAV	CCTTC	1 cut(s)	65
HpyCH4III	ACNGT	2 cut(s)	375, 1006
HpyCH4IV	ACGT	5 cut(s)	309, 513, 668, 866, 960
HpyF10VI	GCNNNNNNNGC	6 cut(s)	563, 583, 596, 927, 1163, 1391
HpyF3I	CTNAG	5 cut(s)	531, 620, 980, 1233, 1362
HpySE526I	ACGT	5 cut(s)	309, 513, 668, 866, 960
Hsp92I	GRCGYC	1 cut(s)	866
Hsp92II	CATG	9 cut(s)	610, 658, 902, 934, 990, 998, 1087, 1147, 1260
HspAI	GCGC	1 cut(s)	324
Ksp22I	TGATCA	1 cut(s)	3
Kzo9I	GATC	4 cut(s)	3, 396, 873, 1090
LguI	GCTCTTC	1 cut(s)	711
LmnI	GCTCC	3 cut(s)	289, 841, 1187
Lsp1109I	GCAGC	5 cut(s)	56, 174, 418, 1081, 1163
LweI	GCATC	3 cut(s)	271, 879, 1070
MaeI	CTAG	4 cut(s)	140, 168, 591, 1250
MaeII	ACGT	5 cut(s)	309, 513, 668, 866, 960
MaeIII	GTNAC	6 cut(s)	318, 633, 649, 664, 689, 961
MalI	GATC	4 cut(s)	5, 398, 875, 1092
MbiI	CCGCTC	1 cut(s)	1106
MboI	GATC	4 cut(s)	3, 396, 873, 1090
MboII	GAAGA	5 cut(s)	698, 1029, 1085, 1151, 1225
MflI	RGATCY	1 cut(s)	1090
MhlI	GDGCHC	2 cut(s)	438, 981
MluCI	AATT	6 cut(s)	45, 97, 157, 201, 772, 1159
MlyI	GAGTC	1 cut(s)	434
MmeI	TCCRAC	1 cut(s)	46
Mph1103I	ATGCAT	1 cut(s)	1085
MroXI	GAANNNNTTC	2 cut(s)	515, 921
MseI	TTAA	1 cut(s)	471
MslI	CAYNNNNRTG	4 cut(s)	720, 897, 903, 1148
MspI	CCGG	4 cut(s)	125, 131, 327, 641
MspR9I	CCNGG	4 cut(s)	125, 131, 835, 1371
Mva1269I	GAATGC	3 cut(s)	508, 559, 923
MvaI	CCWGG	2 cut(s)	835, 1371
MvnI	CGCG	2 cut(s)	279, 324
MwoI	GCNNNNNNNGC	6 cut(s)	563, 583, 596, 927, 1163, 1391
NciI	CCSGG	2 cut(s)	125, 131
NcoI	CCATGG	2 cut(s)	654, 1256
NdeII	GATC	4 cut(s)	3, 396, 873, 1090
NlaIII	CATG	9 cut(s)	610, 658, 902, 934, 990, 998, 1087, 1147, 1260
NlaIV	GGNNCC	3 cut(s)	129, 1189, 1368
NmuCI	GTSAC	5 cut(s)	318, 649, 664, 689, 961
NsiI	ATGCAT	1 cut(s)	1085
NspI	RCATGY	4 cut(s)	610, 902, 990, 1147
PciI	ACATGT	1 cut(s)	606
PciSI	GCTCTTC	1 cut(s)	711
PcsI	WCGNNNNNNNCGW	2 cut(s)	863, 866
PctI	GAATGC	3 cut(s)	508, 559, 923
PdmI	GAANNNNTTC	2 cut(s)	515, 921
PfeI	GAWTC	1 cut(s)	18
PfoI	TCCNGGA	2 cut(s)	123, 833
PkrI	GCNGC	5 cut(s)	71, 164, 408, 1071, 1178
PleI	GAGTC	1 cut(s)	434
PmaCI	CACGTG	1 cut(s)	961
PmlI	CACGTG	1 cut(s)	961
PpsI	GAGTC	1 cut(s)	434
Ppu21I	YACGTR	1 cut(s)	961
PscI	ACATGT	1 cut(s)	606
Psp6I	CCWGG	2 cut(s)	833, 1369
PspCI	CACGTG	1 cut(s)	961
PspFI	CCCAGC	2 cut(s)	232, 541
PspGI	CCWGG	2 cut(s)	833, 1369
PspN4I	GGNNCC	3 cut(s)	129, 1189, 1368
PspPI	GGNCC	1 cut(s)	127
PsuI	RGATCY	1 cut(s)	1090
RseI	CAYNNNNRTG	4 cut(s)	720, 897, 903, 1148
SapI	GCTCTTC	1 cut(s)	711
SaqAI	TTAA	1 cut(s)	471
SatI	GCNGC	5 cut(s)	70, 163, 407, 1070, 1177
Sau3AI	GATC	4 cut(s)	3, 396, 873, 1090
Sau96I	GGNCC	1 cut(s)	127
SchI	GAGTC	1 cut(s)	434
ScrFI	CCNGG	4 cut(s)	125, 131, 835, 1371
SduI	GDGCHC	2 cut(s)	438, 981
SfaNI	GCATC	3 cut(s)	271, 879, 1070
SfcI	CTRYAG	2 cut(s)	13, 600
SgrAI	CRCCGGYG	1 cut(s)	326
SinI	GGWCC	1 cut(s)	127
SmiMI	CAYNNNNRTG	4 cut(s)	720, 897, 903, 1148
SmlI	CTYRAG	1 cut(s)	1115
SmoI	CTYRAG	1 cut(s)	1115
SpeI	ACTAGT	1 cut(s)	167
Sse9I	AATT	6 cut(s)	45, 97, 157, 201, 772, 1159
SsiI	CCGC	7 cut(s)	488, 566, 582, 630, 746, 941, 1106
SspMI	CTAG	4 cut(s)	140, 168, 591, 1250
StyD4I	CCNGG	4 cut(s)	123, 129, 833, 1369
StyI	CCWWGG	3 cut(s)	483, 654, 1256
TaaI	ACNGT	2 cut(s)	375, 1006
TaiI	ACGT	5 cut(s)	312, 516, 671, 869, 963
TaqI	TCGA	2 cut(s)	438, 869
TaqII	GACCGA	1 cut(s)	509
TasI	AATT	6 cut(s)	45, 97, 157, 201, 772, 1159
TfiI	GAWTC	1 cut(s)	18
Tru1I	TTAA	1 cut(s)	471
Tru9I	TTAA	1 cut(s)	471
TscAI	CASTG	5 cut(s)	251, 378, 932, 988, 1011
TseFI	GTSAC	5 cut(s)	318, 649, 664, 689, 961
TseI	GCWGC	5 cut(s)	69, 162, 406, 1069, 1176
Tsp45I	GTSAC	5 cut(s)	318, 649, 664, 689, 961
TspDTI	ATGAA	5 cut(s)	401, 921, 983, 1052, 1166
TspGWI	ACGGA	2 cut(s)	523, 1351
TspRI	CASTG	5 cut(s)	251, 378, 932, 988, 1011
VpaK11BI	GGWCC	1 cut(s)	127
XapI	RAATTY	4 cut(s)	45, 97, 772, 1159
XceI	RCATGY	4 cut(s)	610, 902, 990, 1147
XmiI	GTMKAC	1 cut(s)	603
XmnI	GAANNNNTTC	2 cut(s)	515, 921
XspI	CTAG	4 cut(s)	140, 168, 591, 1250
ZraI	GACGTC	1 cut(s)	867
Zsp2I	ATGCAT	1 cut(s)	1085

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.