MD15G1175900.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010344
Physical Location & Seq
Reverse (-)
13737799 .. 13740312
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1175900.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGAATTTTTCTGATCACTCACAATTTATGCCACCGCAAACGCCTTACAATGGCGGTGGCAGTGACGTGGTTAATTGCTGGCCGCCTGCGTTTTCAATGAACAATGATGACCAGCATTCTCAGGTTGAGCAGCAACCTTTTAAAAGGGCTAGGAATTCTGAAGAACGCCAATCGAATCCGATGCCATACCCGCCCATGAACTATAGGGTTCAGCAGCCGAGTGCCCCGGTTAACAAGGGAGCAAGCAACATTTTCTATAAGACCCGTTTGTGTGTAAATTTTAAATCCGGTATGTGTAGGAATGGTGAGAATTGTAACTATGCACATGGCATTGAAGATATCCGCCATCCCCCGCCCAATTGGCAAGAACTTGTTTCCGTACGCGATGAGGATCGGCCATTGTCTGGTAATTGGGATGATGATCAGAAAATAATCCACAGGATGAAGCTGTGCAAGAAGTTTTATAATGGTGAGGAGTGTCCTTATGGAGATAGGTGTAACTTTCTTCATGAAGATCCATCAAAGTTTAGGGATGATTCGGGTAGGTTTAGGGAGAACACAGCAATAAGCATTGGAACTACAGGAGCACCAGTGGCTCATGGGAGTGGCTCTAATCCTCCTGAGGAAAATAGGCTTGTAAACACGGGTATGGATGCTTCCCGGGGAAATGTGAAGCCAGTGTATTGGAAGACAAAGTTATGTAATAAGTGGGAGACAACGGGTAAATGCCCCTTCGGCGAGAAATGTCACTTTGCTCACGGGCTAACAGAGTTGCAAGTGCCCGGTGGACGTACTGTTGAGGCAGAATTTCTGAATACCCCTCCCTTTTCAATACCCCAAGTTGTTGCTGCTGTCAACTCATCCGCAACTGCAATAGCAGCACTTCCTCCTTTAAATGAAGAAGGGGGGCAGAGGAAGAAGGGCATGCTAAAATGGAAAGGGTCTAAGAAGATCAATCGCATATACGCTGACTGGCTTGATGATGTTCCGCTGGTGCACCAGCACAACTTAGTCGAGAGCTAA

Protein Analysis

341

Amino Acids

38.28

Weight (kDa)

7.59

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_UNK PF25427 85 - 117 7e-07 UNK-like, CCCH zinc finger
zf_CCCH_4 PF18345 91 - 109 7.1e-06 Zinc finger domain
zf-CCCH PF00642 148 - 170 2e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH_4 PF18044 149 - 170 9.4e-08 CCCH-type zinc finger
zf-CCCH PF00642 229 - 254 3.6e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH_DUS3L PF25585 230 - 253 3.3e-07 DUS3L CCCH zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AccB7I CCANNNNNTGG 1 cut(s) 404
AccII CGCG 1 cut(s) 384
AciI CCGC 8 cut(s) 35, 54, 83, 191, 343, 353, 864, 989
AclWI GGATC 2 cut(s) 399, 509
AcoI YGGCCR 2 cut(s) 80, 395
AcsI RAATTY 4 cut(s) 4, 154, 277, 806
AcuI CTGAAG 1 cut(s) 180
AfaI GTAC 2 cut(s) 381, 793
AfiI CCNNNNNNNGG 2 cut(s) 50, 404
AgsI TTSAA 3 cut(s) 96, 335, 831
AjiI CACGTC 1 cut(s) 67
AluBI AGCT 2 cut(s) 448, 1020
AluI AGCT 2 cut(s) 448, 1020
Alw21I GWGCWC 2 cut(s) 589, 999
Alw26I GTCTC 1 cut(s) 707
Alw44I GTGCAC 1 cut(s) 995
AlwI GGATC 2 cut(s) 399, 509
Ama87I CYCGRG 1 cut(s) 660
AoxI GGCC 2 cut(s) 80, 395
ApaLI GTGCAC 1 cut(s) 995
ApeKI GCWGC 4 cut(s) 130, 214, 848, 878
ApoI RAATTY 4 cut(s) 4, 154, 277, 806
AsuC2I CCSGG 4 cut(s) 227, 661, 662, 783
AsuHPI GGTGA 2 cut(s) 317, 482
AvaI CYCGRG 1 cut(s) 660
AxyI CCTNAGG 1 cut(s) 621
BaeGI GKGCMC 3 cut(s) 226, 783, 999
BarI GAAGNNNNNNTAC 2 cut(s) 640, 672
BbsI GAAGAC 1 cut(s) 695
Bbv12I GWGCWC 2 cut(s) 589, 999
BbvI GCAGC 4 cut(s) 142, 226, 835, 890
BccI CCATC 2 cut(s) 354, 526
BcgI CGANNNNNNTGC 2 cut(s) 163, 197
BclI TGATCA 2 cut(s) 13, 421
BcnI CCSGG 4 cut(s) 227, 661, 662, 783
BcoDI GTCTC 1 cut(s) 707
BfaI CTAG 1 cut(s) 150
BfmI CTRYAG 2 cut(s) 202, 579
BglI GCCNNNNNGGC 2 cut(s) 361, 735
BisI GCNGC 5 cut(s) 83, 131, 215, 849, 879
BlsI GCNGC 5 cut(s) 84, 132, 216, 850, 880
Bme1390I CCNGG 4 cut(s) 227, 661, 662, 783
BmeT110I CYCGRG 1 cut(s) 660
BmgBI CACGTC 1 cut(s) 67
BmrFI CCNGG 4 cut(s) 227, 661, 662, 783
BmsI GCATC 2 cut(s) 171, 643
BpiI GAAGAC 1 cut(s) 695
BpuMI CCSGG 4 cut(s) 227, 661, 662, 783
BsaBI GATNNNNATC 2 cut(s) 390, 420
BsaJI CCNNGG 3 cut(s) 225, 660, 661
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 404
Bse1I ACTGG 3 cut(s) 590, 677, 977
Bse21I CCTNAGG 1 cut(s) 621
Bse8I GATNNNNATC 2 cut(s) 390, 420
BseDI CCNNGG 3 cut(s) 225, 660, 661
BseGI GGATG 6 cut(s) 346, 421, 447, 538, 658, 860
BseJI GATNNNNATC 2 cut(s) 390, 420
BseLI CCNNNNNNNGG 2 cut(s) 50, 404
BseMII CTCAG 2 cut(s) 134, 612
BseNI ACTGG 3 cut(s) 590, 677, 977
BseRI GAGGAG 1 cut(s) 488
BseSI GKGCMC 3 cut(s) 226, 783, 999
BseXI GCAGC 4 cut(s) 142, 226, 835, 890
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 2 cut(s) 82, 397
BsiHKAI GWGCWC 2 cut(s) 589, 999
BsiHKCI CYCGRG 1 cut(s) 660
BsiSI CCGG 4 cut(s) 227, 288, 661, 783
BsiWI CGTACG 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 50, 404
BsmAI GTCTC 1 cut(s) 707
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 2 cut(s) 82, 397
BsoBI CYCGRG 1 cut(s) 660
Bsp1286I GDGCHC 4 cut(s) 226, 589, 783, 999
Bsp143I GATC 5 cut(s) 13, 391, 421, 514, 951
BspACI CCGC 8 cut(s) 35, 54, 83, 191, 343, 353, 864, 989
BspANI GGCC 2 cut(s) 82, 397
BspCNI CTCAG 2 cut(s) 133, 613
BspFNI CGCG 1 cut(s) 384
BspHI TCATGA 1 cut(s) 508
BspPI GGATC 2 cut(s) 399, 509
BsrI ACTGG 3 cut(s) 590, 677, 977
BssECI CCNNGG 3 cut(s) 225, 660, 661
BssMI GATC 5 cut(s) 13, 391, 421, 514, 951
Bst4CI ACNGT 1 cut(s) 796
BstC8I GCNNGC 4 cut(s) 80, 87, 244, 926
BstDEI CTNAG 4 cut(s) 120, 621, 945, 1009
BstF5I GGATG 6 cut(s) 346, 421, 447, 538, 658, 860
BstFNI CGCG 1 cut(s) 384
BstKTI GATC 5 cut(s) 16, 394, 424, 517, 954
BstMAI GTCTC 1 cut(s) 707
BstMBI GATC 5 cut(s) 13, 391, 421, 514, 951
BstMWI GCNNNNNNNGC 5 cut(s) 190, 361, 593, 735, 878
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 4 cut(s) 225, 659, 660, 781
BstSFI CTRYAG 2 cut(s) 202, 579
BstSLI GKGCMC 3 cut(s) 226, 783, 999
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 4 cut(s) 142, 226, 835, 890
BstV2I GAAGAC 1 cut(s) 695
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 1 cut(s) 684
BstYI RGATCY 1 cut(s) 514
Bsu36I CCTNAGG 1 cut(s) 621
BsuRI GGCC 2 cut(s) 82, 397
BtgZI GCGATG 1 cut(s) 399
BtrI CACGTC 1 cut(s) 67
BtsCI GGATG 6 cut(s) 346, 421, 447, 538, 658, 860
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 3 cut(s) 67, 597, 684
Cac8I GCNNGC 4 cut(s) 80, 87, 244, 926
CciI TCATGA 1 cut(s) 508
Cfr9I CCCGGG 1 cut(s) 660
Csp6I GTAC 2 cut(s) 380, 792
CspCI CAANNNNNGTGG 2 cut(s) 37, 72
CviAII CATG 5 cut(s) 196, 326, 509, 599, 925
CviQI GTAC 2 cut(s) 380, 792
DdeI CTNAG 4 cut(s) 120, 621, 945, 1009
DpnI GATC 5 cut(s) 15, 393, 423, 516, 953
DpnII GATC 5 cut(s) 13, 391, 421, 514, 951
DraI TTTAAA 3 cut(s) 142, 283, 894
EaeI YGGCCR 2 cut(s) 80, 395
EciI GGCGGA 1 cut(s) 332
Eco32I GATATC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 180
Eco81I CCTNAGG 1 cut(s) 621
Eco88I CYCGRG 1 cut(s) 660
EcoRI GAATTC 1 cut(s) 154
EcoRV GATATC 1 cut(s) 340
FaeI CATG 5 cut(s) 199, 329, 512, 602, 928
FatI CATG 5 cut(s) 195, 325, 508, 598, 924
FauI CCCGC 2 cut(s) 198, 360
FbaI TGATCA 2 cut(s) 13, 421
Fnu4HI GCNGC 5 cut(s) 83, 131, 215, 849, 879
FokI GGATG 6 cut(s) 333, 428, 454, 545, 665, 847
Fsp4HI GCNGC 5 cut(s) 83, 131, 215, 849, 879
FspBI CTAG 1 cut(s) 150
GluI GCNGC 5 cut(s) 83, 131, 215, 849, 879
HaeIII GGCC 2 cut(s) 82, 397
HapII CCGG 4 cut(s) 227, 288, 661, 783
Hin1II CATG 5 cut(s) 199, 329, 512, 602, 928
HincII GTYRAC 2 cut(s) 232, 856
HindII GTYRAC 2 cut(s) 232, 856
HinfI GANTC 2 cut(s) 175, 536
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 4 cut(s) 227, 288, 661, 783
HphI GGTGA 2 cut(s) 317, 482
Hpy166II GTNNAC 5 cut(s) 232, 640, 788, 856, 997
Hpy188I TCNGA 5 cut(s) 13, 160, 180, 426, 813
Hpy188III TCNNGA 3 cut(s) 509, 620, 1015
Hpy8I GTNNAC 5 cut(s) 232, 640, 788, 856, 997
HpyAV CCTTC 3 cut(s) 742, 896, 913
HpyCH4III ACNGT 1 cut(s) 796
HpyCH4IV ACGT 2 cut(s) 66, 790
HpyCH4V TGCA 5 cut(s) 323, 453, 775, 872, 997
HpyF10VI GCNNNNNNNGC 5 cut(s) 190, 361, 593, 735, 878
HpyF3I CTNAG 4 cut(s) 120, 621, 945, 1009
HpySE526I ACGT 2 cut(s) 66, 790
Hsp92II CATG 5 cut(s) 199, 329, 512, 602, 928
Ksp22I TGATCA 2 cut(s) 13, 421
KspAI GTTAAC 1 cut(s) 232
Kzo9I GATC 5 cut(s) 13, 391, 421, 514, 951
LmnI GCTCC 2 cut(s) 239, 584
Lsp1109I GCAGC 4 cut(s) 142, 226, 835, 890
LweI GCATC 2 cut(s) 171, 643
MaeI CTAG 1 cut(s) 150
MaeII ACGT 2 cut(s) 66, 790
MaeIII GTNAC 4 cut(s) 62, 314, 497, 746
MalI GATC 5 cut(s) 15, 393, 423, 516, 953
MboI GATC 5 cut(s) 13, 391, 421, 514, 951
MboII GAAGA 8 cut(s) 173, 347, 497, 524, 700, 911, 928, 961
MfeI CAATTG 1 cut(s) 358
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 4 cut(s) 226, 589, 783, 999
MluCI AATT 9 cut(s) 4, 23, 73, 154, 277, 310, 358, 409, 806
MnlI CCTC 8 cut(s) 382, 466, 616, 627, 793, 831, 897, 906
MseI TTAA 5 cut(s) 72, 141, 231, 282, 893
MslI CAYNNNNRTG 2 cut(s) 603, 647
MspA1I CMGCKG 1 cut(s) 991
MspI CCGG 4 cut(s) 227, 288, 661, 783
MspR9I CCNGG 4 cut(s) 227, 661, 662, 783
MunI CAATTG 1 cut(s) 358
Mva1269I GAATGC 1 cut(s) 115
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 5 cut(s) 190, 361, 593, 735, 878
NciI CCSGG 4 cut(s) 227, 661, 662, 783
NdeII GATC 5 cut(s) 13, 391, 421, 514, 951
NlaIII CATG 5 cut(s) 199, 329, 512, 602, 928
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 2 cut(s) 62, 746
NspI RCATGY 1 cut(s) 928
PaeI GCATGC 1 cut(s) 928
PagI TCATGA 1 cut(s) 508
PctI GAATGC 1 cut(s) 115
PfeI GAWTC 2 cut(s) 175, 536
Pfl23II CGTACG 1 cut(s) 379
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 5 cut(s) 84, 132, 216, 850, 880
PsiI TTATAA 1 cut(s) 465
PspLI CGTACG 1 cut(s) 379
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 2 cut(s) 381, 793
RsaNI GTAC 2 cut(s) 380, 792
RseI CAYNNNNRTG 2 cut(s) 603, 647
SaqAI TTAA 5 cut(s) 72, 141, 231, 282, 893
SatI GCNGC 5 cut(s) 83, 131, 215, 849, 879
Sau3AI GATC 5 cut(s) 13, 391, 421, 514, 951
ScrFI CCNGG 4 cut(s) 227, 661, 662, 783
SduI GDGCHC 4 cut(s) 226, 589, 783, 999
SetI ASST 8 cut(s) 69, 126, 139, 450, 497, 548, 793, 1022
SfaNI GCATC 2 cut(s) 171, 643
SfcI CTRYAG 2 cut(s) 202, 579
SmaI CCCGGG 1 cut(s) 662
SmiMI CAYNNNNRTG 2 cut(s) 603, 647
SphI GCATGC 1 cut(s) 928
Sse9I AATT 9 cut(s) 4, 23, 73, 154, 277, 310, 358, 409, 806
SsiI CCGC 8 cut(s) 35, 54, 83, 191, 343, 353, 864, 989
SspMI CTAG 1 cut(s) 150
StyD4I CCNGG 4 cut(s) 225, 659, 660, 781
TaaI ACNGT 1 cut(s) 796
TaiI ACGT 2 cut(s) 69, 793
TaqI TCGA 2 cut(s) 173, 1014
TasI AATT 9 cut(s) 4, 23, 73, 154, 277, 310, 358, 409, 806
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 2 cut(s) 175, 536
Tru1I TTAA 5 cut(s) 72, 141, 231, 282, 893
Tru9I TTAA 5 cut(s) 72, 141, 231, 282, 893
TscAI CASTG 3 cut(s) 67, 597, 684
TseFI GTSAC 2 cut(s) 62, 746
TseI GCWGC 4 cut(s) 130, 214, 848, 878
Tsp45I GTSAC 2 cut(s) 62, 746
TspDTI ATGAA 7 cut(s) 17, 113, 212, 458, 497, 525, 912
TspGWI ACGGA 1 cut(s) 367
TspMI CCCGGG 1 cut(s) 660
TspRI CASTG 3 cut(s) 67, 597, 684
Van91I CCANNNNNTGG 1 cut(s) 404
VneI GTGCAC 1 cut(s) 995
XapI RAATTY 4 cut(s) 4, 154, 277, 806
XceI RCATGY 1 cut(s) 928
XmaI CCCGGG 1 cut(s) 660
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.