MD15G1266900.v1.1

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013371
Physical Location & Seq
Reverse (-)
22947252 .. 22949459
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1266900.v1.1.491

Sequence Viewer

Length: 648 bp
ATGACAACCAACAGCCACCAAATTGTTGAAGACGATCATCAGATGCTACTGATCTCTCACTTGTACTCGGCCTCTGATGTCTACACTCAAATTGTACCCCCCCAAGGAGCCTCAAAGCCGGCAAGACGACGTCGCAGGAAGAGCAAAGGTGGAGAAGCTGGTGGTGTTGGGCTTAAGAAGAGGAAGCTCACAGCTGAACAAGTAGACCTTCTTGAGCTCAATTTTGGCAACGAACACAAACTGGAGTCTGAGAAGAAAGACAGGCTTGCTTCTGACCTGGGTCTTGATCCTCGTCAAGTTGCTGTTTGGTTCCAAAACCGTAGGGCTCGTTGGAAGAACAAGAAGTTGGAGGAAGAGTACTCAACCTTAAAGAAAGAAAATGAAATAACTGTTGCTGAGAAATGTAGACTTGAATCCGAGATGTCGAAGCTCAAGGATCAACTCTCGGAGGCTGAGAAGGAGATCCAACGGCTCTTGCAGCGGGTCGATCACGGCGGATCCAGCAACAGTCCGAGCTCCTCGCTCTCCATGGAAGCCATTGATCCTCCATTTTTAGGAGAATTTGGGGTGGAAGAGTACGATGATGTTTTTTACATGCCCCAGAACAATTACATTCATGGCATGGAATGGATGAACCTGTATATGTGA

Protein Analysis

216

Amino Acids

24.76

Weight (kDa)

6.12

Isoelectric Point (pI)

57.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 60 - 113 1.5e-16 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18550 AT4G36740 AT4G36740
fragaria_vesca FvH4_1g13930 FvH4_1g13930
malus_domestica MD02G1152500.v1.1 MD15G1266900.v1.1
prunus_persica Prupe.7G149700_v2.0.a1
pyrus_communis pycom02g12010
rosa_chinensis RchiOBHm_Chr2g0102221
rosa_laevigata RLG00000017139
rosa_multiflora Rmu_sc0005611.1_g000012 Rmu_sc0006727.1_g000013
rosa_roxburghii Rroxscaffold_2G00140640
rosa_rugosa Rorug02G0105900
rosa_samantha Rh2AG153800 Rh2CG159400
rosa_wichuraiana Rw2G012020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 133
AccI GTMKAC 3 cut(s) 81, 204, 406
AciI CCGC 2 cut(s) 481, 495
AclWI GGATC 6 cut(s) 281, 444, 457, 492, 505, 536
AcsI RAATTY 1 cut(s) 560
AcyI GRCGYC 1 cut(s) 130
AfaI GTAC 4 cut(s) 65, 96, 359, 578
AfiI CCNNNNNNNGG 2 cut(s) 104, 554
AflII CTTAAG 1 cut(s) 173
AgsI TTSAA 2 cut(s) 29, 413
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 6 cut(s) 158, 187, 194, 217, 430, 516
AluI AGCT 6 cut(s) 158, 187, 194, 217, 430, 516
Alw21I GWGCWC 2 cut(s) 219, 518
AlwI GGATC 6 cut(s) 281, 444, 457, 492, 505, 536
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 1 cut(s) 478
ApoI RAATTY 1 cut(s) 560
BamHI GGATCC 1 cut(s) 497
BanII GRGCYC 3 cut(s) 219, 328, 518
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 2 cut(s) 219, 518
BbvI GCAGC 1 cut(s) 490
BceAI ACGGC 2 cut(s) 485, 508
BciT130I CCWGG 1 cut(s) 278
BfrI CTTAAG 1 cut(s) 173
BisI GCNGC 1 cut(s) 479
BlsI GCNGC 1 cut(s) 480
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 278
BmiI GGNNCC 3 cut(s) 109, 311, 499
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 33
BoxI GACNNNNGTC 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 263
BpuEI CTTGAG 2 cut(s) 233, 416
BsaHI GRCGYC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 103, 277, 528
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 554
Bse118I RCCGGY 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 246
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 103, 277, 528
BseGI GGATG 1 cut(s) 636
BseLI CCNNNNNNNGG 2 cut(s) 104, 554
BseMII CTCAG 3 cut(s) 240, 387, 444
BseNI ACTGG 1 cut(s) 246
BseRI GAGGAG 1 cut(s) 508
BseXI GCAGC 1 cut(s) 490
BshFI GGCC 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 219, 518
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 104, 554
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 3 cut(s) 219, 328, 518
Bsp143I GATC 8 cut(s) 34, 51, 286, 436, 462, 487, 497, 541
Bsp19I CCATGG 1 cut(s) 528
BspACI CCGC 2 cut(s) 481, 495
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 3 cut(s) 241, 388, 445
BspLI GGNNCC 3 cut(s) 109, 311, 499
BspPI GGATC 6 cut(s) 281, 444, 457, 492, 505, 536
BspQI GCTCTTC 1 cut(s) 134
BspTI CTTAAG 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 118
BsrI ACTGG 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 3 cut(s) 103, 277, 528
BssMI GATC 8 cut(s) 34, 51, 286, 436, 462, 487, 497, 541
BssNI GRCGYC 1 cut(s) 130
BssT1I CCWWGG 2 cut(s) 103, 528
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 3 cut(s) 320, 391, 509
Bst6I CTCTTC 4 cut(s) 134, 173, 348, 567
BstACI GRCGYC 1 cut(s) 130
BstAFI CTTAAG 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 120, 267
BstDEI CTNAG 3 cut(s) 249, 396, 453
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 1 cut(s) 636
BstKTI GATC 8 cut(s) 37, 54, 289, 439, 465, 490, 500, 544
BstMBI GATC 8 cut(s) 34, 51, 286, 436, 462, 487, 497, 541
BstMWI GCNNNNNNNGC 3 cut(s) 141, 478, 501
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 598
BstPAI GACNNNNGTC 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 490
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 2 cut(s) 462, 497
BstYI RGATCY 2 cut(s) 462, 497
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 1 cut(s) 636
Cac8I GCNNGC 2 cut(s) 120, 267
Cfr10I RCCGGY 1 cut(s) 118
Csp6I GTAC 4 cut(s) 64, 95, 358, 577
CviAII CATG 4 cut(s) 529, 595, 617, 622
CviQI GTAC 4 cut(s) 64, 95, 358, 577
DdeI CTNAG 3 cut(s) 249, 396, 453
DpnI GATC 8 cut(s) 36, 53, 288, 438, 464, 489, 499, 543
DpnII GATC 8 cut(s) 34, 51, 286, 436, 462, 487, 497, 541
Eam1104I CTCTTC 4 cut(s) 134, 173, 348, 567
EarI CTCTTC 4 cut(s) 134, 173, 348, 567
EciI GGCGGA 1 cut(s) 510
Ecl136II GAGCTC 2 cut(s) 217, 516
Eco130I CCWWGG 2 cut(s) 103, 528
Eco24I GRGCYC 3 cut(s) 219, 328, 518
Eco53kI GAGCTC 2 cut(s) 217, 516
EcoICRI GAGCTC 2 cut(s) 217, 516
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 2 cut(s) 103, 528
EcoT38I GRGCYC 3 cut(s) 219, 328, 518
ErhI CCWWGG 2 cut(s) 103, 528
FaeI CATG 4 cut(s) 532, 598, 620, 625
FaiI YATR 6 cut(s) 530, 596, 618, 623, 642, 644
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 249, 281
FatI CATG 4 cut(s) 528, 594, 616, 621
FauI CCCGC 1 cut(s) 474
FblI GTMKAC 3 cut(s) 81, 204, 406
Fnu4HI GCNGC 1 cut(s) 479
FokI GGATG 1 cut(s) 643
FriOI GRGCYC 3 cut(s) 219, 328, 518
Fsp4HI GCNGC 1 cut(s) 479
GluI GCNGC 1 cut(s) 479
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 119
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 4 cut(s) 532, 598, 620, 625
HinfI GANTC 2 cut(s) 245, 413
HpaII CCGG 1 cut(s) 119
Hpy166II GTNNAC 3 cut(s) 82, 205, 407
Hpy188I TCNGA 7 cut(s) 42, 76, 250, 274, 418, 448, 513
Hpy188III TCNNGA 2 cut(s) 212, 284
Hpy8I GTNNAC 3 cut(s) 82, 205, 407
Hpy99I CGWCG 2 cut(s) 132, 135
HpyAV CCTTC 2 cut(s) 218, 451
HpyCH4III ACNGT 3 cut(s) 320, 391, 509
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 1 cut(s) 478
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 478, 501
HpyF3I CTNAG 3 cut(s) 249, 396, 453
HpySE526I ACGT 1 cut(s) 130
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 4 cut(s) 532, 598, 620, 625
KroI GCCGGC 1 cut(s) 118
KroNI GCCGGC 1 cut(s) 120
Kzo9I GATC 8 cut(s) 34, 51, 286, 436, 462, 487, 497, 541
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 2 cut(s) 107, 521
LpnPI CCDG 9 cut(s) 121, 132, 144, 227, 247, 263, 290, 514, 614
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 1 cut(s) 33
MaeII ACGT 1 cut(s) 130
MalI GATC 8 cut(s) 36, 53, 288, 438, 464, 489, 499, 543
MboI GATC 8 cut(s) 34, 51, 286, 436, 462, 487, 497, 541
MboII GAAGA 7 cut(s) 41, 151, 190, 265, 346, 365, 584
MflI RGATCY 2 cut(s) 462, 497
MhlI GDGCHC 3 cut(s) 219, 328, 518
MluCI AATT 5 cut(s) 21, 90, 220, 560, 607
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 3 cut(s) 311, 327, 490
MnlI CCTC 8 cut(s) 82, 121, 174, 300, 343, 442, 529, 555
MroNI GCCGGC 1 cut(s) 118
MseI TTAA 2 cut(s) 174, 368
MspA1I CMGCKG 2 cut(s) 194, 481
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 3 cut(s) 141, 478, 501
NaeI GCCGGC 1 cut(s) 120
NcoI CCATGG 1 cut(s) 528
NdeII GATC 8 cut(s) 34, 51, 286, 436, 462, 487, 497, 541
NgoMIV GCCGGC 1 cut(s) 118
NlaIII CATG 4 cut(s) 532, 598, 620, 625
NlaIV GGNNCC 3 cut(s) 109, 311, 499
NmeAIII GCCGAG 1 cut(s) 47
NspI RCATGY 1 cut(s) 598
PciSI GCTCTTC 1 cut(s) 134
PdiI GCCGGC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 129
PkrI GCNGC 1 cut(s) 480
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PshAI GACNNNNGTC 1 cut(s) 279
Psp124BI GAGCTC 2 cut(s) 219, 518
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 3 cut(s) 109, 311, 499
PsuI RGATCY 2 cut(s) 462, 497
PsyI GACNNNGTC 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 4 cut(s) 65, 96, 359, 578
RsaNI GTAC 4 cut(s) 64, 95, 358, 577
SacI GAGCTC 2 cut(s) 219, 518
SapI GCTCTTC 1 cut(s) 134
SaqAI TTAA 2 cut(s) 174, 368
SatI GCNGC 1 cut(s) 479
Sau3AI GATC 8 cut(s) 34, 51, 286, 436, 462, 487, 497, 541
ScaI AGTACT 1 cut(s) 359
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 3 cut(s) 219, 328, 518
SfaNI GCATC 1 cut(s) 33
SmlI CTYRAG 3 cut(s) 173, 212, 431
SmoI CTYRAG 3 cut(s) 173, 212, 431
Sse9I AATT 5 cut(s) 21, 90, 220, 560, 607
SsiI CCGC 2 cut(s) 481, 495
SstI GAGCTC 2 cut(s) 219, 518
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 2 cut(s) 103, 528
TaaI ACNGT 3 cut(s) 320, 391, 509
TaiI ACGT 1 cut(s) 133
TaqI TCGA 2 cut(s) 425, 486
TasI AATT 5 cut(s) 21, 90, 220, 560, 607
TatI WGTACW 2 cut(s) 63, 357
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 2 cut(s) 174, 368
Tru9I TTAA 2 cut(s) 174, 368
TseI GCWGC 1 cut(s) 478
TspDTI ATGAA 3 cut(s) 396, 605, 647
Tth111I GACNNNGTC 1 cut(s) 129
Vha464I CTTAAG 1 cut(s) 173
XapI RAATTY 1 cut(s) 560
XceI RCATGY 1 cut(s) 598
XmiI GTMKAC 3 cut(s) 81, 204, 406
ZraI GACGTC 1 cut(s) 131
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.