MD15G1377000.v1.1

Blue copper

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011789
Physical Location & Seq
Reverse (-)
46127884 .. 46129243
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1377000.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGCCAGAATTGGGGGTTTAGCCCTAGTTGTTGCCATGGTCATGGTGCCAAGCTGCGTAGCCACTGTCTACACCGTTGGAGACTCCTCTGGGTGGGCTAGTGGAGTGGATTATACCACTTGGACTAGTGGCAAGACCTTTAAAGTTGGTGACAGCCTAGTTTTCAACTATGGAAGTGGACACACGGTGGATGAAGTGACTGCTAGTGACTACAAAACATGCACTGTGGGAAATTCAATTACAAGTGATAGCAGCGGAGCCACCACCATCCTCCTCAAGACTGCCGGGACTCATAACTACATTTGCGCTACGATTGGGCATTGCGGTATGGGGATGAAGCTTTCTGTGTCTGTAGGGTCAGGATCAACCGCAGCACCATCCAGTAATTCAGCATCCGGTGGTTCCGGCGGTAACTCTACAGTTACAACCCCTGCAAGTACCACATCCTCAACAACTGGTGTTGGGATTAGCCACTCTAGCTCTCTTGTTGTGGCTTTGTGGAGTGTCATTAGTTTGGTTGGTTTTTGTATGTTCCTTCTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

182

Amino Acids

18.01

Weight (kDa)

6.37

Isoelectric Point (pI)

21.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 32 - 112 3.8e-22 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22480 AT1G72230
fragaria_vesca FvH4_5g36400
malus_domestica MD08G1189500.v1.1 MD15G1377000.v1.1
prunus_persica Prupe.1G523700_v2.0.a1
pyrus_communis pycom08g16280 pycom15g33670
rosa_chinensis RchiOBHm_Chr7g0238371
rosa_laevigata RLG00000000914
rosa_multiflora Rmu_sc0001371.1_g000024
rosa_roxburghii Rroxscaffold_3G00223070
rosa_rugosa Rorug07G0312300
rosa_samantha Rh7AG467100 Rh7BG437900 Rh7CG485200 Rh7DG453600
rosa_wichuraiana Rw7G038730 Rw7G038800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 69
AciI CCGC 4 cut(s) 255, 324, 369, 408
AclWI GGATC 1 cut(s) 370
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 232
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 1 cut(s) 439
AfiI CCNNNNNNNGG 2 cut(s) 12, 93
AgsI TTSAA 2 cut(s) 166, 237
AhlI ACTAGT 1 cut(s) 125
AluBI AGCT 3 cut(s) 54, 340, 480
AluI AGCT 3 cut(s) 54, 340, 480
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 1 cut(s) 370
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 54, 252, 371
ApoI RAATTY 1 cut(s) 232
AspLEI GCGC 1 cut(s) 308
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 1 cut(s) 161
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 46
BbvI GCAGC 3 cut(s) 41, 264, 383
BccI CCATC 2 cut(s) 275, 385
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 75
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 6 cut(s) 26, 99, 126, 158, 204, 477
BfmI CTRYAG 2 cut(s) 351, 417
BisI GCNGC 3 cut(s) 55, 253, 372
BlsI GCNGC 3 cut(s) 56, 254, 373
Bme1390I CCNGG 1 cut(s) 286
BmiI GGNNCC 3 cut(s) 48, 259, 403
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 1 cut(s) 401
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 286
BsaJI CCNNGG 1 cut(s) 36
BsaWI WCCGGW 1 cut(s) 395
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 93
Bse1I ACTGG 2 cut(s) 381, 460
Bse3DI GCAATG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 6 cut(s) 196, 267, 339, 377, 392, 443
BseLI CCNNNNNNNGG 2 cut(s) 12, 93
BseMI GCAATG 1 cut(s) 319
BseNI ACTGG 2 cut(s) 381, 460
BseRI GAGGAG 2 cut(s) 76, 263
BseXI GCAGC 3 cut(s) 41, 264, 383
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 46
BsiSI CCGG 3 cut(s) 285, 396, 405
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 12, 93
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 301
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 362
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 4 cut(s) 255, 324, 369, 408
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 3 cut(s) 48, 259, 403
BspPI GGATC 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 319
BsrI ACTGG 2 cut(s) 381, 460
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 1 cut(s) 362
BssT1I CCWWGG 1 cut(s) 36
Bst4CI ACNGT 5 cut(s) 67, 76, 187, 226, 421
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 6 cut(s) 196, 267, 339, 377, 392, 443
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 1 cut(s) 365
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 1 cut(s) 362
BstMWI GCNNNNNNNGC 1 cut(s) 477
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 284
BstSFI CTRYAG 2 cut(s) 351, 417
BstV1I GCAGC 3 cut(s) 41, 264, 383
BstXI CCANNNNNNTGG 1 cut(s) 43
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 6 cut(s) 196, 267, 339, 377, 392, 443
BtsIMutI CAGTG 2 cut(s) 63, 222
CfoI GCGC 1 cut(s) 308
Csp6I GTAC 1 cut(s) 438
CviAII CATG 3 cut(s) 37, 43, 219
CviQI GTAC 1 cut(s) 438
DpnI GATC 1 cut(s) 364
DpnII GATC 1 cut(s) 362
DraI TTTAAA 1 cut(s) 142
DraIII CACNNNGTG 1 cut(s) 187
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 3 cut(s) 40, 46, 222
FaiI YATR 8 cut(s) 38, 44, 114, 171, 220, 294, 329, 530
FaqI GGGAC 1 cut(s) 301
FatI CATG 3 cut(s) 36, 42, 218
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 55, 253, 372
FokI GGATG 6 cut(s) 203, 254, 346, 364, 379, 430
Fsp4HI GCNGC 3 cut(s) 55, 253, 372
FspBI CTAG 6 cut(s) 26, 99, 126, 158, 204, 477
GlaI GCGC 1 cut(s) 307
GluI GCNGC 3 cut(s) 55, 253, 372
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 285, 396, 405
HhaI GCGC 1 cut(s) 308
Hin1II CATG 3 cut(s) 40, 46, 222
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 2 cut(s) 83, 289
HpaII CCGG 3 cut(s) 285, 396, 405
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 70, 179
Hpy188III TCNNGA 2 cut(s) 277, 360
Hpy8I GTNNAC 2 cut(s) 70, 179
HpyAV CCTTC 1 cut(s) 545
HpyCH4III ACNGT 5 cut(s) 67, 76, 187, 226, 421
HpyCH4V TGCA 2 cut(s) 222, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 477
Hsp92II CATG 3 cut(s) 40, 46, 222
HspAI GCGC 1 cut(s) 306
Kzo9I GATC 1 cut(s) 362
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 9 cut(s) 19, 75, 298, 345, 394, 409, 418, 441, 444
Lsp1109I GCAGC 3 cut(s) 41, 264, 383
LweI GCATC 1 cut(s) 401
MaeI CTAG 6 cut(s) 26, 99, 126, 158, 204, 477
MaeIII GTNAC 5 cut(s) 149, 196, 206, 410, 421
MalI GATC 1 cut(s) 364
MboI GATC 1 cut(s) 362
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 9, 232, 237, 385
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 77, 283
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 4 cut(s) 97, 281, 284, 457
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 141, 544
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 3 cut(s) 285, 396, 405
MspR9I CCNGG 1 cut(s) 286
MwoI GCNNNNNNNGC 1 cut(s) 477
NciI CCSGG 1 cut(s) 286
NcoI CCATGG 1 cut(s) 36
NdeII GATC 1 cut(s) 362
NlaIII CATG 3 cut(s) 40, 46, 222
NlaIV GGNNCC 3 cut(s) 48, 259, 403
NmuCI GTSAC 3 cut(s) 149, 196, 206
NspI RCATGY 1 cut(s) 222
PkrI GCNGC 3 cut(s) 56, 254, 373
PleI GAGTC 2 cut(s) 77, 283
PpsI GAGTC 2 cut(s) 77, 283
PspN4I GGNNCC 3 cut(s) 48, 259, 403
RsaI GTAC 1 cut(s) 439
RsaNI GTAC 1 cut(s) 438
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 2 cut(s) 141, 544
SatI GCNGC 3 cut(s) 55, 253, 372
Sau3AI GATC 1 cut(s) 362
SchI GAGTC 2 cut(s) 77, 283
ScrFI CCNGG 1 cut(s) 286
SetI ASST 4 cut(s) 56, 140, 342, 482
SfaNI GCATC 1 cut(s) 401
SfcI CTRYAG 2 cut(s) 351, 417
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SpeI ACTAGT 1 cut(s) 125
Sse9I AATT 4 cut(s) 9, 232, 237, 385
SsiI CCGC 4 cut(s) 255, 324, 369, 408
SspMI CTAG 6 cut(s) 26, 99, 126, 158, 204, 477
StyD4I CCNGG 1 cut(s) 284
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 5 cut(s) 67, 76, 187, 226, 421
TasI AATT 4 cut(s) 9, 232, 237, 385
Tru1I TTAA 2 cut(s) 141, 544
Tru9I TTAA 2 cut(s) 141, 544
TscAI CASTG 2 cut(s) 70, 229
TseFI GTSAC 3 cut(s) 149, 196, 206
TseI GCWGC 3 cut(s) 54, 252, 371
Tsp45I GTSAC 3 cut(s) 149, 196, 206
TspDTI ATGAA 2 cut(s) 207, 350
TspRI CASTG 2 cut(s) 70, 229
XapI RAATTY 1 cut(s) 232
XceI RCATGY 1 cut(s) 222
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 6 cut(s) 26, 99, 126, 158, 204, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.