MD15G1411500.v1.1

FabA-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007315
Physical Location & Seq
Forward (+)
51095040 .. 51098997
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1411500.v1.1.491

Sequence Viewer

Length: 672 bp
ATGTCGACCGCAGCTTACTCCGCCTCTCTCACTTGCCACCCCCCGCTCCTTCACCGACCAAATGCTCCCTCCCGAATCTCTGTCGCCGTCGCGATTCCCAGATCTCTGCCGCCGTTGCCCCATTTGAAAACTAAGAGGAGGAACTCAACCACCACCTATTGCTCCGCCGACGCTGCGAAAGACAAAGACTCCACTCCCATTGAGCTCAGATATGAGGCTTTTCCTACCGTCATGGACATCAATCAAATTCGTGAAATTTTGCCTCACCGGTTTCCGTTTCTGTTAGTGGATCGAGTGATTGAGTACAATGCTGGAGTTTCAGCTGTTGCAATCAAGAATGTCACCATCAATGATAATTTCTTTCCTGGGCATTTCCCCGAGAGGCCGATTATGCCTGGTGTTCTCATGGTTGAGGCAATGGCACAGGTCGGTGGCTTGGTTATGCTGCAACCAGAAGTGGGAGGCTCTCGTGACACTTTCTTTTTCGCTGGAATCGACAAAGTTCGATTCAGGAAACCAGTGATTGCCGGCGACACGTTAGTGATGAGAATGACGCTTATTAAGTTGCAGAAACGCTTTGGAATTGCAAAGATGGAAGGCAAGGCCTACGTTGGAGGTGAGGTCGTATGTGAGGGGGAGTTTTTGATGGCTACTGGTACCGGTGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00780 ko01100 ko01212 map00061 map00780 map01100 map01212
Pfam Domains
Protein Families
FabA

Protein Analysis

224

Amino Acids

24.45

Weight (kDa)

9.19

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ApeP-like PF22817 82 - 212 3.4e-06 ApeP dehydratase
FabA PF07977 87 - 212 1.7e-34 FabA-like domain
ApeI-like PF22818 108 - 206 8e-14 ApeI dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 656
AccBSI CCGCTC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 92
AciI CCGC 5 cut(s) 9, 21, 44, 110, 165
AclWI GGATC 1 cut(s) 297
AcsI RAATTY 2 cut(s) 246, 255
AfaI GTAC 2 cut(s) 305, 658
AfiI CCNNNNNNNGG 1 cut(s) 458
AflIII ACRYGT 1 cut(s) 534
AgeI ACCGGT 2 cut(s) 267, 659
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 2 cut(s) 364, 394
AleI CACNNNNGTG 1 cut(s) 539
AluBI AGCT 3 cut(s) 14, 205, 323
AluI AGCT 3 cut(s) 14, 205, 323
Alw21I GWGCWC 1 cut(s) 207
AlwI GGATC 1 cut(s) 297
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 2 cut(s) 383, 603
ApeKI GCWGC 3 cut(s) 11, 173, 445
ApoI RAATTY 2 cut(s) 246, 255
AsiGI ACCGGT 2 cut(s) 267, 659
Asp718I GGTACC 1 cut(s) 656
AsuHPI GGTGA 4 cut(s) 44, 257, 334, 629
AvaI CYCGRG 1 cut(s) 377
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 1 cut(s) 207
BauI CACGAG 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 3 cut(s) 23, 160, 432
BccI CCATC 3 cut(s) 353, 586, 640
BceAI ACGGC 2 cut(s) 71, 97
BciT130I CCWGG 2 cut(s) 366, 396
BglII AGATCT 1 cut(s) 101
BisI GCNGC 4 cut(s) 12, 110, 174, 446
BlsI GCNGC 4 cut(s) 13, 111, 175, 447
Bme1390I CCNGG 2 cut(s) 366, 396
BmeT110I CYCGRG 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 2 cut(s) 366, 396
BpmI CTGGAG 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 365
BsaWI WCCGGW 2 cut(s) 267, 659
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 606, 636
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse118I RCCGGY 3 cut(s) 267, 527, 659
Bse1I ACTGG 2 cut(s) 518, 658
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 2 cut(s) 366, 396
BseDI CCNNGG 1 cut(s) 365
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 220
BseNI ACTGG 2 cut(s) 518, 658
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 3 cut(s) 23, 160, 432
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 385, 605
BshNI GGYRCC 1 cut(s) 656
BshTI ACCGGT 2 cut(s) 267, 659
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 3 cut(s) 268, 528, 660
BslI CCNNNNNNNGG 1 cut(s) 458
BsnI GGCC 2 cut(s) 385, 605
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 2 cut(s) 101, 289
Bsp68I TCGCGA 1 cut(s) 92
BspACI CCGC 5 cut(s) 9, 21, 44, 110, 165
BspANI GGCC 2 cut(s) 385, 605
BspCNI CTCAG 1 cut(s) 219
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 658
BspPI GGATC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 656
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 3 cut(s) 267, 527, 659
BsrI ACTGG 2 cut(s) 518, 658
BssAI RCCGGY 3 cut(s) 267, 527, 659
BssECI CCNNGG 1 cut(s) 365
BssMI GATC 2 cut(s) 101, 289
BssSI CACGAG 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 2 cut(s) 366, 396
Bst4CI ACNGT 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 529
BstDEI CTNAG 2 cut(s) 132, 206
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 2 cut(s) 104, 292
BstMBI GATC 2 cut(s) 101, 289
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 20, 115, 173, 391
BstNI CCWGG 2 cut(s) 366, 396
BstSCI CCNGG 2 cut(s) 364, 394
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 3 cut(s) 23, 160, 432
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 2 cut(s) 385, 605
BtsIMutI CAGTG 1 cut(s) 525
BtuMI TCGCGA 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 529
Cfr10I RCCGGY 3 cut(s) 267, 527, 659
CseI GACGC 2 cut(s) 179, 562
Csp6I GTAC 2 cut(s) 304, 657
CspAI ACCGGT 2 cut(s) 267, 659
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 2 cut(s) 232, 406
CviJI RGCY 9 cut(s) 14, 205, 218, 323, 385, 435, 465, 605, 650
CviKI_1 RGCY 9 cut(s) 14, 205, 218, 323, 385, 435, 465, 605, 650
CviQI GTAC 2 cut(s) 304, 657
DdeI CTNAG 2 cut(s) 132, 206
DpnI GATC 2 cut(s) 103, 291
DpnII GATC 2 cut(s) 101, 289
EciI GGCGGA 2 cut(s) 10, 154
Ecl136II GAGCTC 1 cut(s) 205
Eco147I AGGCCT 1 cut(s) 605
Eco24I GRGCYC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 364, 394
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 2 cut(s) 235, 409
FaiI YATR 6 cut(s) 213, 233, 392, 407, 443, 628
FatI CATG 2 cut(s) 231, 405
FauI CCCGC 1 cut(s) 51
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 12, 110, 174, 446
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 4 cut(s) 12, 110, 174, 446
GluI GCNGC 4 cut(s) 12, 110, 174, 446
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 2 cut(s) 385, 605
HapII CCGG 3 cut(s) 268, 528, 660
HgaI GACGC 2 cut(s) 179, 562
Hin1II CATG 2 cut(s) 235, 409
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 5 cut(s) 75, 94, 188, 492, 507
HpaII CCGG 3 cut(s) 268, 528, 660
HphI GGTGA 4 cut(s) 44, 257, 334, 629
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 6 cut(s) 72, 91, 251, 334, 470, 511
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 92, 173
HpyAV CCTTC 2 cut(s) 59, 590
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 2 cut(s) 536, 609
HpyCH4V TGCA 4 cut(s) 329, 448, 568, 587
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 115, 173, 391
HpyF3I CTNAG 2 cut(s) 132, 206
HpySE526I ACGT 2 cut(s) 536, 609
Hsp92II CATG 2 cut(s) 235, 409
KpnI GGTACC 1 cut(s) 660
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Kzo9I GATC 2 cut(s) 101, 289
LmnI GCTCC 3 cut(s) 51, 70, 167
Lsp1109I GCAGC 3 cut(s) 23, 160, 432
MaeII ACGT 2 cut(s) 536, 609
MaeIII GTNAC 3 cut(s) 340, 470, 665
MalI GATC 2 cut(s) 103, 291
MbiI CCGCTC 1 cut(s) 46
MboI GATC 2 cut(s) 101, 289
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 4 cut(s) 246, 255, 355, 582
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 592
MroNI GCCGGC 1 cut(s) 527
MseI TTAA 1 cut(s) 561
MslI CAYNNNNRTG 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 3 cut(s) 268, 528, 660
MspR9I CCNGG 2 cut(s) 366, 396
MvaI CCWGG 2 cut(s) 366, 396
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 20, 115, 173, 391
NaeI GCCGGC 1 cut(s) 529
NdeII GATC 2 cut(s) 101, 289
NgoMIV GCCGGC 1 cut(s) 527
NlaIII CATG 2 cut(s) 235, 409
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 3 cut(s) 340, 470, 665
NruI TCGCGA 1 cut(s) 92
OliI CACNNNNGTG 1 cut(s) 539
PceI AGGCCT 1 cut(s) 605
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PdiI GCCGGC 1 cut(s) 529
PfeI GAWTC 4 cut(s) 75, 94, 492, 507
PinAI ACCGGT 2 cut(s) 267, 659
PkrI GCNGC 4 cut(s) 13, 111, 175, 447
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 364, 394
PspGI CCWGG 2 cut(s) 364, 394
PspN4I GGNNCC 1 cut(s) 658
PsuI RGATCY 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 323
RruI TCGCGA 1 cut(s) 92
RsaI GTAC 2 cut(s) 305, 658
RsaNI GTAC 2 cut(s) 304, 657
RseI CAYNNNNRTG 1 cut(s) 539
SacI GAGCTC 1 cut(s) 207
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 4 cut(s) 12, 110, 174, 446
Sau3AI GATC 2 cut(s) 101, 289
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 366, 396
SduI GDGCHC 1 cut(s) 207
SetI ASST 9 cut(s) 16, 158, 207, 325, 429, 539, 612, 619, 624
SmiMI CAYNNNNRTG 1 cut(s) 539
Sse9I AATT 4 cut(s) 246, 255, 355, 582
SseBI AGGCCT 1 cut(s) 605
SsiI CCGC 5 cut(s) 9, 21, 44, 110, 165
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 1 cut(s) 605
StyD4I CCNGG 2 cut(s) 364, 394
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 2 cut(s) 539, 612
TaqI TCGA 4 cut(s) 5, 292, 495, 505
TasI AATT 4 cut(s) 246, 255, 355, 582
TatI WGTACW 1 cut(s) 303
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 4 cut(s) 75, 94, 492, 507
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TscAI CASTG 1 cut(s) 525
TseFI GTSAC 3 cut(s) 340, 470, 665
TseI GCWGC 3 cut(s) 11, 173, 445
Tsp45I GTSAC 3 cut(s) 340, 470, 665
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 1 cut(s) 525
XapI RAATTY 2 cut(s) 246, 255
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.