pycom08g19160

FabA-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007315
Physical Location & Seq
Forward (+)
19197221 .. 19198988
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19160.1

Sequence Viewer

Length: 489 bp
ATGTCGACCGCAGCTTCCTCCACCTCCTTCACTTGCCACTCCCCGCTCCTTCCCCGCCCAAATGCTCCATCCGGGATCTCTGTCGCCGTCGCGATTCCCAGGTGTCTGCCGCCGTTGCCGCATTTGAAAACTAAGAGGAGGAGTTCAACCACCACCTATTGCTCCGCCGACGCTGCGAAAGATAAAGATTCCACCCCCATTGAGCTCAGATATGAGGCTTTTCCTACCGTCATGGACATCAATCAAATTCGTGATATTTTGCCTCACCGGTTTCCGTTTCTGTTAGTGGATAGAGTGATTGAATACAATGCTGGAGTTTCAGCTGTTGCAATCAAGAATGTCACCATCAATGATAATTTCTTTCCTGGGCATTTCCCCGAGAGGCCGATTATGCCTGGTGTTCTCATGGTTGAGATTTTGGATCGGGTTTCTAAACTAGAGCATGTAGATTGGAGTCCTATAAATGAGAAGGAAGCTTGGTCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00780 ko01100 ko01212 map00061 map00780 map01100 map01212
Pfam Domains
Protein Families
FabA

Protein Analysis

163

Amino Acids

18.04

Weight (kDa)

6.51

Isoelectric Point (pI)

53.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FabA PF07977 87 - 138 5.8e-17 FabA-like domain
ApeI-like PF22818 108 - 144 1.5e-07 ApeI dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 92
AciI CCGC 6 cut(s) 9, 44, 55, 110, 119, 165
AclWI GGATC 2 cut(s) 83, 429
AcsI RAATTY 1 cut(s) 246
AgeI ACCGGT 1 cut(s) 267
AgsI TTSAA 3 cut(s) 127, 147, 302
AjnI CCWGG 3 cut(s) 98, 364, 394
AluBI AGCT 4 cut(s) 14, 205, 323, 476
AluI AGCT 4 cut(s) 14, 205, 323, 476
Alw21I GWGCWC 1 cut(s) 207
AlwI GGATC 2 cut(s) 83, 429
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 1 cut(s) 383
ApeKI GCWGC 2 cut(s) 11, 173
ApoI RAATTY 1 cut(s) 246
AsiGI ACCGGT 1 cut(s) 267
AsuC2I CCSGG 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 257, 334
AvaI CYCGRG 1 cut(s) 377
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 23, 160
BccI CCATC 2 cut(s) 76, 353
BceAI ACGGC 2 cut(s) 71, 97
BciT130I CCWGG 3 cut(s) 100, 366, 396
BcnI CCSGG 1 cut(s) 73
BfaI CTAG 1 cut(s) 437
BisI GCNGC 4 cut(s) 12, 110, 119, 174
BlsI GCNGC 4 cut(s) 13, 111, 120, 175
Bme1390I CCNGG 4 cut(s) 73, 100, 366, 396
BmeT110I CYCGRG 1 cut(s) 377
BmrFI CCNGG 4 cut(s) 73, 100, 366, 396
BpmI CTGGAG 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 73
BsaJI CCNNGG 2 cut(s) 98, 365
BsaWI WCCGGW 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 267
BseBI CCWGG 3 cut(s) 100, 366, 396
BseDI CCNNGG 2 cut(s) 98, 365
BseGI GGATG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 220
BseRI GAGGAG 2 cut(s) 151, 154
BseXI GCAGC 2 cut(s) 23, 160
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 385
BshTI ACCGGT 1 cut(s) 267
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 2 cut(s) 72, 268
BsnI GGCC 1 cut(s) 385
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 2 cut(s) 75, 421
Bsp68I TCGCGA 1 cut(s) 92
BspACI CCGC 6 cut(s) 9, 44, 55, 110, 119, 165
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 1 cut(s) 219
BspFNI CGCG 1 cut(s) 92
BspPI GGATC 2 cut(s) 83, 429
BsrBI CCGCTC 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 2 cut(s) 98, 365
BssMI GATC 2 cut(s) 75, 421
Bst2UI CCWGG 3 cut(s) 100, 366, 396
Bst4CI ACNGT 1 cut(s) 229
BstDEI CTNAG 2 cut(s) 132, 206
BstF5I GGATG 1 cut(s) 68
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 2 cut(s) 78, 424
BstMBI GATC 2 cut(s) 75, 421
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 115, 118, 173, 391
BstNI CCWGG 3 cut(s) 100, 366, 396
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 4 cut(s) 71, 98, 364, 394
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 2 cut(s) 23, 160
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuRI GGCC 1 cut(s) 385
BtsCI GGATG 1 cut(s) 68
BtuMI TCGCGA 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 267
CseI GACGC 1 cut(s) 179
CspAI ACCGGT 1 cut(s) 267
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 3 cut(s) 232, 406, 443
CviJI RGCY 6 cut(s) 14, 205, 218, 323, 385, 476
CviKI_1 RGCY 6 cut(s) 14, 205, 218, 323, 385, 476
DdeI CTNAG 2 cut(s) 132, 206
DpnI GATC 2 cut(s) 77, 423
DpnII GATC 2 cut(s) 75, 421
EciI GGCGGA 1 cut(s) 154
Ecl136II GAGCTC 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 205
EcoRII CCWGG 3 cut(s) 98, 364, 394
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 3 cut(s) 235, 409, 446
FaiI YATR 6 cut(s) 213, 233, 392, 407, 444, 461
FatI CATG 3 cut(s) 231, 405, 442
FauI CCCGC 2 cut(s) 51, 62
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 12, 110, 119, 174
FokI GGATG 1 cut(s) 55
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 4 cut(s) 12, 110, 119, 174
FspBI CTAG 1 cut(s) 437
GluI GCNGC 4 cut(s) 12, 110, 119, 174
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 1 cut(s) 385
HapII CCGG 2 cut(s) 72, 268
HgaI GACGC 1 cut(s) 179
Hin1II CATG 3 cut(s) 235, 409, 446
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 3 cut(s) 94, 188, 454
HpaII CCGG 2 cut(s) 72, 268
HphI GGTGA 2 cut(s) 257, 334
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 209, 484
Hpy188III TCNNGA 3 cut(s) 91, 251, 334
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 92, 173
HpyAV CCTTC 3 cut(s) 37, 59, 463
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 118, 173, 391
HpyF3I CTNAG 2 cut(s) 132, 206
Hsp92II CATG 3 cut(s) 235, 409, 446
Kzo9I GATC 2 cut(s) 75, 421
LmnI GCTCC 3 cut(s) 51, 70, 167
LpnPI CCDG 9 cut(s) 85, 85, 112, 281, 297, 351, 378, 381, 408
Lsp1109I GCAGC 2 cut(s) 23, 160
MaeI CTAG 1 cut(s) 437
MaeIII GTNAC 1 cut(s) 340
MalI GATC 2 cut(s) 77, 423
MbiI CCGCTC 1 cut(s) 46
MboI GATC 2 cut(s) 75, 421
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 2 cut(s) 246, 355
MlyI GAGTC 1 cut(s) 463
MnlI CCTC 7 cut(s) 28, 34, 129, 132, 208, 273, 375
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 2 cut(s) 72, 268
MspR9I CCNGG 4 cut(s) 73, 100, 366, 396
MvaI CCWGG 3 cut(s) 100, 366, 396
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 115, 118, 173, 391
NciI CCSGG 1 cut(s) 73
NdeII GATC 2 cut(s) 75, 421
NlaIII CATG 3 cut(s) 235, 409, 446
NmuCI GTSAC 1 cut(s) 340
NruI TCGCGA 1 cut(s) 92
NspI RCATGY 1 cut(s) 446
PfeI GAWTC 2 cut(s) 94, 188
PfoI TCCNGGA 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 267
PkrI GCNGC 4 cut(s) 13, 111, 120, 175
PleI GAGTC 1 cut(s) 462
PpsI GAGTC 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 3 cut(s) 98, 364, 394
PspGI CCWGG 3 cut(s) 98, 364, 394
PsuI RGATCY 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 323
RruI TCGCGA 1 cut(s) 92
SacI GAGCTC 1 cut(s) 207
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 4 cut(s) 12, 110, 119, 174
Sau3AI GATC 2 cut(s) 75, 421
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 4 cut(s) 73, 100, 366, 396
SduI GDGCHC 1 cut(s) 207
SetI ASST 7 cut(s) 16, 26, 104, 158, 207, 325, 478
Sse9I AATT 2 cut(s) 246, 355
SsiI CCGC 6 cut(s) 9, 44, 55, 110, 119, 165
SspMI CTAG 1 cut(s) 437
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 4 cut(s) 71, 98, 364, 394
TaaI ACNGT 1 cut(s) 229
TaqI TCGA 1 cut(s) 5
TasI AATT 2 cut(s) 246, 355
TauI GCSGC 2 cut(s) 112, 121
TfiI GAWTC 2 cut(s) 94, 188
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 11, 173
Tsp45I GTSAC 1 cut(s) 340
TspGWI ACGGA 1 cut(s) 264
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.