MD15G1420400.v1.1

helicase MAGATAMA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003781
Physical Location & Seq
Forward (+)
52198953 .. 52201061
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1420400.v1.1.491

Sequence Viewer

Length: 600 bp
ATGCTCTTGTATCATAATTTAGTGGATAAATATCCAGAGCTTAAATCAAGTCAGCGGTTCACGATTATATCCCCGTATCGTGGACAAGTAACTCTCTTCAAAGAACGTTTCAGAAGTACTTTCGGAGTGGAGTCTGACAAATTCGTGGATATTACTACTATTGATGGTTGCCAGGGACGTGAAAAGGATGTTGCAATATTTTCATGTGTCAGGACAAACGAGCATGGAAAAATTGGGTTTCTGTCTGATTTTCGGCGAATGAATGTTGCGATCACTAGAGCAAAATCTTCTGTCTTGGTAGTGGGATCTGCATCAACATTAAGGAAGCGCGATGAGCATTGGAATAACCTTATCGAAAGCGCTGAAAAGAGGGATTGTTTGCTTAAGGTCTCACAGCCATACACTTCATTTTTCAGTGATGAGAATCTGGAAACCATGGCTATCAAGAAAGAATTCTCATTGGAGGAGGTTGAGAATGATGAGGTGGATAACTATGCAGGTTCAAACAATTTTGGGGGTGCCGACCCGGCGGAGGGAGAGGAGAACGATTATGGAGACGGGGGTATGGACATGGATGATGGAGGCAACGGCGATGACTGA

Protein Analysis

200

Amino Acids

22.4

Weight (kDa)

4.71

Isoelectric Point (pI)

29.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_12 PF13087 3 - 104 1.5e-33 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 518
AccII CGCG 1 cut(s) 330
AciI CCGC 2 cut(s) 55, 530
AclI AACGTT 1 cut(s) 106
AclWI GGATC 1 cut(s) 313
AcsI RAATTY 2 cut(s) 140, 452
AfaI GTAC 1 cut(s) 118
AfeI AGCGCT 1 cut(s) 361
AfiI CCNNNNNNNGG 3 cut(s) 80, 532, 533
AflII CTTAAG 1 cut(s) 383
AgsI TTSAA 2 cut(s) 100, 504
AjiI CACGTC 1 cut(s) 179
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
Alw26I GTCTC 2 cut(s) 394, 549
AlwI GGATC 1 cut(s) 313
Aor51HI AGCGCT 1 cut(s) 361
ApoI RAATTY 2 cut(s) 140, 452
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 2 cut(s) 330, 362
AsuC2I CCSGG 1 cut(s) 527
BanI GGYRCC 1 cut(s) 518
BccI CCATC 2 cut(s) 158, 572
BciT130I CCWGG 1 cut(s) 173
BcnI CCSGG 1 cut(s) 527
BcoDI GTCTC 2 cut(s) 394, 549
BfaI CTAG 1 cut(s) 276
BfoI RGCGCY 1 cut(s) 363
BfrI CTTAAG 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 488
BglI GCCNNNNNGGC 1 cut(s) 527
BmcAI AGTACT 1 cut(s) 118
Bme1390I CCNGG 2 cut(s) 173, 527
BmgBI CACGTC 1 cut(s) 179
BmiI GGNNCC 1 cut(s) 520
BmrFI CCNGG 2 cut(s) 173, 527
BmsI GCATC 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 527
BsaBI GATNNNNATC 3 cut(s) 30, 310, 423
BsaI GGTCTC 1 cut(s) 394
BsaJI CCNNGG 2 cut(s) 172, 435
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 532, 533
Bse8I GATNNNNATC 3 cut(s) 30, 310, 423
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 2 cut(s) 172, 435
BseGI GGATG 2 cut(s) 193, 580
BseJI GATNNNNATC 3 cut(s) 30, 310, 423
BseLI CCNNNNNNNGG 3 cut(s) 80, 532, 533
BseRI GAGGAG 2 cut(s) 479, 554
Bsh1236I CGCG 1 cut(s) 330
BshNI GGYRCC 1 cut(s) 518
BsiSI CCGG 1 cut(s) 527
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 3 cut(s) 80, 532, 533
BsmAI GTCTC 2 cut(s) 394, 549
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 189
Bso31I GGTCTC 1 cut(s) 394
Bsp143I GATC 2 cut(s) 270, 305
Bsp19I CCATGG 1 cut(s) 435
BspACI CCGC 2 cut(s) 55, 530
BspFNI CGCG 1 cut(s) 330
BspLI GGNNCC 1 cut(s) 520
BspMI ACCTGC 1 cut(s) 488
BspPI GGATC 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 518
BspTI CTTAAG 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 394
BssECI CCNNGG 2 cut(s) 172, 435
BssMI GATC 2 cut(s) 270, 305
BssT1I CCWWGG 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 173
Bst6I CTCTTC 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 383
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 2 cut(s) 193, 580
BstFNI CGCG 1 cut(s) 330
BstH2I RGCGCY 1 cut(s) 363
BstHHI GCGC 2 cut(s) 330, 362
BstKTI GATC 2 cut(s) 273, 308
BstMAI GTCTC 2 cut(s) 394, 549
BstMBI GATC 2 cut(s) 270, 305
BstMWI GCNNNNNNNGC 2 cut(s) 334, 527
BstNI CCWGG 1 cut(s) 173
BstSCI CCNGG 2 cut(s) 171, 525
BstUI CGCG 1 cut(s) 330
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 435
BtgZI GCGATG 1 cut(s) 345
BtrI CACGTC 1 cut(s) 179
BtsCI GGATG 2 cut(s) 193, 580
BtsIMutI CAGTG 1 cut(s) 421
BveI ACCTGC 1 cut(s) 488
CfoI GCGC 2 cut(s) 330, 362
Csp6I GTAC 1 cut(s) 117
CviAII CATG 4 cut(s) 204, 224, 436, 571
CviJI RGCY 3 cut(s) 40, 397, 440
CviKI_1 RGCY 3 cut(s) 40, 397, 440
CviQI GTAC 1 cut(s) 117
DpnI GATC 2 cut(s) 272, 307
DpnII GATC 2 cut(s) 270, 305
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
EciI GGCGGA 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 394
Eco47III AGCGCT 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 4 cut(s) 207, 227, 439, 574
FaqI GGGAC 1 cut(s) 189
FatI CATG 4 cut(s) 203, 223, 435, 570
FokI GGATG 2 cut(s) 200, 587
FspBI CTAG 1 cut(s) 276
GlaI GCGC 2 cut(s) 329, 361
HaeII RGCGCY 1 cut(s) 363
HapII CCGG 1 cut(s) 527
HhaI GCGC 2 cut(s) 330, 362
Hin1II CATG 4 cut(s) 207, 227, 439, 574
Hin6I GCGC 2 cut(s) 328, 360
HinP1I GCGC 2 cut(s) 328, 360
HinfI GANTC 2 cut(s) 131, 424
HpaII CCGG 1 cut(s) 527
Hpy166II GTNNAC 2 cut(s) 60, 83
Hpy188I TCNGA 4 cut(s) 113, 125, 136, 247
Hpy188III TCNNGA 5 cut(s) 35, 61, 211, 428, 445
Hpy8I GTNNAC 2 cut(s) 60, 83
HpyCH4IV ACGT 2 cut(s) 106, 178
HpyCH4V TGCA 3 cut(s) 194, 311, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 334, 527
HpySE526I ACGT 2 cut(s) 106, 178
Hsp92II CATG 4 cut(s) 207, 227, 439, 574
HspAI GCGC 2 cut(s) 328, 360
Kzo9I GATC 2 cut(s) 270, 305
LpnPI CCDG 7 cut(s) 48, 158, 185, 196, 413, 483, 540
LweI GCATC 1 cut(s) 320
MaeI CTAG 1 cut(s) 276
MaeII ACGT 2 cut(s) 106, 178
MaeIII GTNAC 1 cut(s) 88
MalI GATC 2 cut(s) 272, 307
MboI GATC 2 cut(s) 270, 305
MboII GAAGA 2 cut(s) 88, 279
MflI RGATCY 1 cut(s) 305
MluCI AATT 5 cut(s) 16, 140, 231, 452, 508
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 7 cut(s) 363, 457, 460, 475, 526, 532, 575
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 3 cut(s) 42, 320, 384
MspA1I CMGCKG 1 cut(s) 55
MspCI CTTAAG 1 cut(s) 383
MspI CCGG 1 cut(s) 527
MspR9I CCNGG 2 cut(s) 173, 527
MvaI CCWGG 1 cut(s) 173
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 2 cut(s) 334, 527
NciI CCSGG 1 cut(s) 527
NcoI CCATGG 1 cut(s) 435
NdeII GATC 2 cut(s) 270, 305
NlaIII CATG 4 cut(s) 207, 227, 439, 574
NlaIV GGNNCC 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 1 cut(s) 424
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
Psp1406I AACGTT 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 520
PsuI RGATCY 1 cut(s) 305
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
SaqAI TTAA 3 cut(s) 42, 320, 384
Sau3AI GATC 2 cut(s) 270, 305
ScaI AGTACT 1 cut(s) 118
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 173, 527
SetI ASST 8 cut(s) 42, 109, 181, 351, 390, 471, 486, 502
SfaNI GCATC 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 5 cut(s) 16, 140, 231, 452, 508
SsiI CCGC 2 cut(s) 55, 530
SspI AATATT 1 cut(s) 198
SspMI CTAG 1 cut(s) 276
StyD4I CCNGG 2 cut(s) 171, 525
StyI CCWWGG 1 cut(s) 435
TaiI ACGT 2 cut(s) 109, 181
TaqI TCGA 1 cut(s) 354
TasI AATT 5 cut(s) 16, 140, 231, 452, 508
TatI WGTACW 1 cut(s) 116
TfiI GAWTC 1 cut(s) 424
Tru1I TTAA 3 cut(s) 42, 320, 384
Tru9I TTAA 3 cut(s) 42, 320, 384
TscAI CASTG 1 cut(s) 421
TspDTI ATGAA 3 cut(s) 192, 275, 396
TspRI CASTG 1 cut(s) 421
Vha464I CTTAAG 1 cut(s) 383
XapI RAATTY 2 cut(s) 140, 452
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 1 cut(s) 276
ZrmI AGTACT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.