Rorug04G0080000

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000204
Physical Location & Seq
Forward (+)
12215077 .. 12217803
2727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0080000.1

Sequence Viewer

Length: 843 bp
ATGGCGACGAAATCCGATTCCCCAAAGAGGGAGTGGTCCCTGAACGACTTCGAGATCGGAAAGCCTCTCGGCAAAGGCAAGTTCGGGCGGGTCTACGTGGCCCGAGAAGTCAAGAGCAAGTACGTGGTGGCGTTGAAGGTGTTGTTCAAGAAGCAGATAAAGAAGTACAAGATTCACCACCAGCTGAAGAGAGAGATGGAGATCCAGACCAGTCTCAGACACCCCAACATTCTTCGCCTCTACGGTTGGTTCCACGACGACGAACGCATCGTCTTGATCCTCGAGTACGCCCACGGCGGCGAGCTGTATGGGCTGCTCAGGGAGAATACTCATCTCTCTGAGAAACAGACCGCCACGTATATACTGAGCCTGGCGCAGGCTTTGGCTTATTGTCACGAGAAGCATGTGATTCATAGGGATATCAAGCCGGAAAATTTGTTGCTTGATCATGAGGGTCGACTGAAAATTGCAGACTTTGGATGGTCTGTACAGTCAAGCAGCAAGAGACGAACAATGTGTGGAACATTAGATTATTTAGCACCAGAGATGGTGGAGAACAGAGCTCATGATTATGCAGTGGATAACTGGACTTTGGGAATTCTCTGCTATGAGTTTCTTTATGGCACTCCTCCATTTGAGGCTGAAAGTCAAAGAGATACTTTCAAAAGGATTACGAACATTGACCTGAGCTTTCCTTCTAGCCCTCAAGTTTCTGCAGAAGCTAAACATCTCATTAGCCAGCTTCTGGTGAAGGACTCCTCTAAGAGGCTTTCTCTTCAGAAGATCATGGAACACCCTTGGATAATGAAGAATGCAGATCCTTCCGGTATCTGCAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

280

Amino Acids

32.42

Weight (kDa)

9.07

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 17 - 268 2.7e-77 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 18 - 264 3.2e-45 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 108 - 214 6.2e-07 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 745
AccI GTMKAC 2 cut(s) 93, 457
AciI CCGC 3 cut(s) 88, 297, 351
AclWI GGATC 3 cut(s) 196, 271, 812
AcsI RAATTY 2 cut(s) 433, 597
AcuI CTGAAG 2 cut(s) 206, 761
AfaI GTAC 4 cut(s) 122, 167, 287, 489
AfiI CCNNNNNNNGG 5 cut(s) 27, 28, 376, 745, 765
AgsI TTSAA 3 cut(s) 136, 148, 664
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 6 cut(s) 184, 304, 563, 690, 722, 742
AluI AGCT 6 cut(s) 184, 304, 563, 690, 722, 742
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 2 cut(s) 218, 499
AlwI GGATC 3 cut(s) 196, 271, 812
AlwNI CAGNNNCTG 1 cut(s) 745
Ama87I CYCGRG 2 cut(s) 102, 281
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 2 cut(s) 313, 498
ApoI RAATTY 2 cut(s) 433, 597
ArsI GACNNNNNNTTYG 2 cut(s) 255, 287
Asp700I GAANNNNTTC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 2 cut(s) 36, 100
AsuHPI GGTGA 2 cut(s) 167, 760
AvaI CYCGRG 2 cut(s) 102, 281
AvaII GGWCC 1 cut(s) 36
BanII GRGCYC 1 cut(s) 565
BauI CACGAG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 2 cut(s) 300, 510
BccI CCATC 3 cut(s) 190, 474, 541
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 1 cut(s) 371
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 218, 499
BfaI CTAG 2 cut(s) 699, 841
BfmI CTRYAG 1 cut(s) 714
BisI GCNGC 3 cut(s) 298, 314, 499
BlsI GCNGC 3 cut(s) 299, 315, 500
Bme1390I CCNGG 1 cut(s) 371
Bme18I GGWCC 1 cut(s) 36
BmeT110I CYCGRG 2 cut(s) 102, 281
BmgT120I GGNCC 2 cut(s) 36, 100
BmiI GGNNCC 2 cut(s) 38, 251
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 1 cut(s) 276
BplI GAGNNNNNCTC 2 cut(s) 757, 789
Bpu10I CCTNAGC 2 cut(s) 317, 686
BpuEI CTTGAG 1 cut(s) 690
BsaAI YACGTR 3 cut(s) 97, 124, 357
BsaBI GATNNNNATC 1 cut(s) 200
BsaJI CCNNGG 2 cut(s) 292, 797
BsaWI WCCGGW 1 cut(s) 824
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 28, 376, 745, 765
Bse1I ACTGG 2 cut(s) 210, 590
Bse8I GATNNNNATC 1 cut(s) 200
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 2 cut(s) 292, 797
BseGI GGATG 1 cut(s) 485
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 5 cut(s) 27, 28, 376, 745, 765
BseMII CTCAG 5 cut(s) 229, 330, 331, 356, 677
BseNI ACTGG 2 cut(s) 210, 590
BseRI GAGGAG 2 cut(s) 618, 748
BseXI GCAGC 2 cut(s) 300, 510
BshFI GGCC 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 565
BsiHKCI CYCGRG 2 cut(s) 102, 281
BsiSI CCGG 2 cut(s) 428, 825
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 5 cut(s) 27, 28, 376, 745, 765
BsmAI GTCTC 2 cut(s) 218, 499
BsmBI CGTCTC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 1 cut(s) 101
BsoBI CYCGRG 2 cut(s) 102, 281
Bsp1286I GDGCHC 1 cut(s) 565
Bsp1407I TGTACA 1 cut(s) 487
Bsp143I GATC 6 cut(s) 54, 201, 276, 445, 783, 817
BspACI CCGC 3 cut(s) 88, 297, 351
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 5 cut(s) 228, 330, 331, 357, 678
BspHI TCATGA 2 cut(s) 448, 565
BspLI GGNNCC 2 cut(s) 38, 251
BspMAI CTGCAG 1 cut(s) 718
BspPI GGATC 3 cut(s) 196, 271, 812
BsrGI TGTACA 1 cut(s) 487
BsrI ACTGG 2 cut(s) 210, 590
BssECI CCNNGG 2 cut(s) 292, 797
BssMI GATC 6 cut(s) 54, 201, 276, 445, 783, 817
BssSI CACGAG 1 cut(s) 395
BssT1I CCWWGG 1 cut(s) 797
Bst2BI CACGAG 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 2 cut(s) 245, 492
Bst6I CTCTTC 2 cut(s) 182, 780
BstAUI TGTACA 1 cut(s) 487
BstBAI YACGTR 3 cut(s) 97, 124, 357
BstC8I GCNNGC 3 cut(s) 302, 378, 740
BstDEI CTNAG 6 cut(s) 215, 317, 339, 365, 686, 762
BstDSI CCRYGG 1 cut(s) 292
BstENI CCTNNNNNAGG 2 cut(s) 374, 763
BstF5I GGATG 1 cut(s) 485
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 6 cut(s) 57, 204, 279, 448, 786, 820
BstMAI GTCTC 2 cut(s) 218, 499
BstMBI GATC 6 cut(s) 54, 201, 276, 445, 783, 817
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 369
BstSFI CTRYAG 1 cut(s) 714
BstV1I GCAGC 2 cut(s) 300, 510
BstX2I RGATCY 2 cut(s) 201, 817
BstYI RGATCY 2 cut(s) 201, 817
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 292
BtsCI GGATG 1 cut(s) 485
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 3 cut(s) 302, 378, 740
CaiI CAGNNNCTG 1 cut(s) 745
CciI TCATGA 2 cut(s) 448, 565
CfoI GCGC 1 cut(s) 376
Cfr13I GGNCC 2 cut(s) 36, 100
Csp6I GTAC 4 cut(s) 121, 166, 286, 488
CviAII CATG 4 cut(s) 404, 449, 566, 787
CviQI GTAC 4 cut(s) 121, 166, 286, 488
DdeI CTNAG 6 cut(s) 215, 317, 339, 365, 686, 762
DpnI GATC 6 cut(s) 56, 203, 278, 447, 785, 819
DpnII GATC 6 cut(s) 54, 201, 276, 445, 783, 817
Eam1104I CTCTTC 2 cut(s) 182, 780
EarI CTCTTC 2 cut(s) 182, 780
Ecl136II GAGCTC 1 cut(s) 563
Eco130I CCWWGG 1 cut(s) 797
Eco24I GRGCYC 1 cut(s) 565
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 563
Eco57I CTGAAG 2 cut(s) 206, 761
Eco88I CYCGRG 2 cut(s) 102, 281
EcoICRI GAGCTC 1 cut(s) 563
EcoNI CCTNNNNNAGG 2 cut(s) 374, 763
EcoRI GAATTC 1 cut(s) 597
EcoRII CCWGG 1 cut(s) 369
EcoRV GATATC 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 797
EcoT38I GRGCYC 1 cut(s) 565
ErhI CCWWGG 1 cut(s) 797
Esp3I CGTCTC 1 cut(s) 499
FaeI CATG 4 cut(s) 407, 452, 569, 790
FalI AAGNNNNNCTT 2 cut(s) 643, 675
FaqI GGGAC 1 cut(s) 22
FatI CATG 4 cut(s) 403, 448, 565, 786
FauI CCCGC 1 cut(s) 81
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 2 cut(s) 93, 457
Fnu4HI GCNGC 3 cut(s) 298, 314, 499
FokI GGATG 1 cut(s) 492
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 3 cut(s) 298, 314, 499
FspBI CTAG 2 cut(s) 699, 841
GlaI GCGC 1 cut(s) 375
GluI GCNGC 3 cut(s) 298, 314, 499
HaeIII GGCC 1 cut(s) 101
HapII CCGG 2 cut(s) 428, 825
HhaI GCGC 1 cut(s) 376
Hin1II CATG 4 cut(s) 407, 452, 569, 790
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 1 cut(s) 458
HindII GTYRAC 1 cut(s) 458
HinfI GANTC 4 cut(s) 17, 172, 409, 755
HpaII CCGG 2 cut(s) 428, 825
HphI GGTGA 2 cut(s) 167, 760
Hpy166II GTNNAC 2 cut(s) 94, 458
Hpy188I TCNGA 5 cut(s) 16, 59, 218, 340, 780
Hpy188III TCNNGA 8 cut(s) 52, 112, 148, 205, 274, 395, 449, 566
Hpy8I GTNNAC 2 cut(s) 94, 458
Hpy99I CGWCG 3 cut(s) 10, 260, 263
HpyAV CCTTC 4 cut(s) 130, 705, 745, 831
HpyCH4III ACNGT 2 cut(s) 245, 492
HpyCH4IV ACGT 3 cut(s) 96, 123, 356
HpyCH4V TGCA 5 cut(s) 470, 575, 716, 815, 834
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 6 cut(s) 215, 317, 339, 365, 686, 762
HpySE526I ACGT 3 cut(s) 96, 123, 356
Hsp92II CATG 4 cut(s) 407, 452, 569, 790
HspAI GCGC 1 cut(s) 374
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 6 cut(s) 54, 201, 276, 445, 783, 817
Lsp1109I GCAGC 2 cut(s) 300, 510
LweI GCATC 1 cut(s) 276
MaeI CTAG 2 cut(s) 699, 841
MaeII ACGT 3 cut(s) 96, 123, 356
MaeIII GTNAC 1 cut(s) 392
MalI GATC 6 cut(s) 56, 203, 278, 447, 785, 819
MboI GATC 6 cut(s) 54, 201, 276, 445, 783, 817
MboII GAAGA 5 cut(s) 199, 224, 767, 793, 820
MflI RGATCY 2 cut(s) 201, 817
MhlI GDGCHC 1 cut(s) 565
MluCI AATT 3 cut(s) 433, 465, 597
MlyI GAGTC 1 cut(s) 749
MroXI GAANNNNTTC 1 cut(s) 47
MslI CAYNNNNRTG 1 cut(s) 570
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 2 cut(s) 428, 825
MspR9I CCNGG 1 cut(s) 371
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 310
NdeII GATC 6 cut(s) 54, 201, 276, 445, 783, 817
NlaIII CATG 4 cut(s) 407, 452, 569, 790
NlaIV GGNNCC 2 cut(s) 38, 251
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 392
NspI RCATGY 1 cut(s) 407
PaeR7I CTCGAG 1 cut(s) 281
PagI TCATGA 2 cut(s) 448, 565
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 817
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 3 cut(s) 17, 172, 409
PflMI CCANNNNNTGG 1 cut(s) 745
PkrI GCNGC 3 cut(s) 299, 315, 500
PleI GAGTC 1 cut(s) 749
PpsI GAGTC 1 cut(s) 749
Ppu21I YACGTR 3 cut(s) 97, 124, 357
Psp124BI GAGCTC 1 cut(s) 565
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 38, 251
PspPI GGNCC 2 cut(s) 36, 100
PspXI VCTCGAGB 1 cut(s) 281
PstI CTGCAG 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 745
PsuI RGATCY 2 cut(s) 201, 817
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 4 cut(s) 122, 167, 287, 489
RsaNI GTAC 4 cut(s) 121, 166, 286, 488
RseI CAYNNNNRTG 1 cut(s) 570
SacI GAGCTC 1 cut(s) 565
SalI GTCGAC 1 cut(s) 456
SatI GCNGC 3 cut(s) 298, 314, 499
Sau3AI GATC 6 cut(s) 54, 201, 276, 445, 783, 817
Sau96I GGNCC 2 cut(s) 36, 100
SchI GAGTC 1 cut(s) 749
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 1 cut(s) 565
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 714
Sfr274I CTCGAG 1 cut(s) 281
SinI GGWCC 1 cut(s) 36
SlaI CTCGAG 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 2 cut(s) 281, 705
SmoI CTYRAG 2 cut(s) 281, 705
Sse9I AATT 3 cut(s) 433, 465, 597
SsiI CCGC 3 cut(s) 88, 297, 351
SspMI CTAG 2 cut(s) 699, 841
SstI GAGCTC 1 cut(s) 565
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 1 cut(s) 797
TaaI ACNGT 2 cut(s) 245, 492
TaiI ACGT 3 cut(s) 99, 126, 359
TaqI TCGA 3 cut(s) 51, 282, 457
TasI AATT 3 cut(s) 433, 465, 597
TatI WGTACW 2 cut(s) 165, 487
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 3 cut(s) 17, 172, 409
TscAI CASTG 1 cut(s) 582
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 2 cut(s) 313, 498
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 401, 821
TspRI CASTG 1 cut(s) 582
Van91I CCANNNNNTGG 1 cut(s) 745
VpaK11BI GGWCC 1 cut(s) 36
XagI CCTNNNNNAGG 2 cut(s) 374, 763
XapI RAATTY 2 cut(s) 433, 597
XceI RCATGY 1 cut(s) 407
XcmI CCANNNNNNNNNTGG 1 cut(s) 30
XhoI CTCGAG 1 cut(s) 281
XmiI GTMKAC 2 cut(s) 93, 457
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 2 cut(s) 699, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.