AT3G25270

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000204
Physical Location & Seq
Reverse (-)
9203934 .. 9204965
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25270.1

Sequence Viewer

Length: 1032 bp
ATGGATCCAGAAATTCAACCACCCCCAGGAAAAGCAGAGATTAAAGCTAAGATATGGAAACTCAAAACGGCGCCAAAAATCAAACACTTCCTCTGGAAGCTACTCTCAGGAGCCTTAGCCACAGGAGACAATCTAAAACGAAGACATATACGGAATCACCCGCAATGTCACCGGTGCTGCCAAGAGGACGAAACGTCTCAACACTTATTCTTTGATTGTTTTTATGCCCAACAGGTTTGGCGAGCTTCAGGGATACCACACCAAGAGTTACGAACTACAGGAATTACAATGGAGACAAAGATGGAGCTGCTGTTATCGAGTTGTTTAGCCAACAGACAACCACAACTTTTTAATTTGGCCATCTGGATCTTATGGAGACTATGGAAGAGCAGAAATCAATTGGTGTTTCAACAAAAAAGCATATCATGGCAAAACACCCTACAACGTGCACGGAATGATGTTCAAGAGTGGGAAGACACAAACACTTATGTTCAGAGTCTCAACCAACAAGTGCATTCATCAAGACATCAACAACCAACTATGGCACGTACGAAGTGGCAACGGCCGCCAAGCACTTGGATAAAGTACAATTATGATGGAGCTTTCAACCACCAAACACGAAATGCGAAAGCTGGCTGGCTAATGAGAGATGAGAATGGAGTATACATGGGTTCGGGTCAAGCAATAGGATCAACAACATCAGACTCATTGGAGAGTGAATTTCAGGCTTTGATTATAGCAATGCAACATGCTTGGAGCCAAGGCTATCGCAAAGTTATCTTTGAAGGGGACAGTAAACAAGTGGAAGAGCTTATGAACAACGAAAAGCTAAACTTTGGAAGATTCAACTGGATAAGAGAAGGCAGATTTTGGCAAAAACGATTTGAGGAGGCTGTCTTCAAATGGGTTCCAAGAACAAACAACCAACCTGCAGATATCCTCGCAAAGCATCATCTTCAACCAAATCAGAGTTTTAAATTTCACTACTATGTACCCGCTTTCATAACATCAACTTTGTATTATGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Exo_endo_phos_2 RVT_3 zf-RVT

Protein Analysis

343

Amino Acids

40.58

Weight (kDa)

9.53

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 11 - 80 1.8e-20 zinc-binding in reverse transcriptase
RVT_3 PF13456 197 - 318 1.4e-39 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 937
AccB1I GGYRCC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 663
AciI CCGC 3 cut(s) 161, 566, 996
AclWI GGATC 3 cut(s) 12, 374, 697
AcoI YGGCCR 2 cut(s) 357, 563
AcsI RAATTY 3 cut(s) 12, 719, 977
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 3 cut(s) 550, 587, 993
AfiI CCNNNNNNNGG 1 cut(s) 26
AgeI ACCGGT 1 cut(s) 171
AgsI TTSAA 8 cut(s) 17, 410, 464, 607, 785, 847, 901, 959
AhdI GACNNNNNGTC 1 cut(s) 193
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 8 cut(s) 47, 100, 245, 307, 602, 632, 811, 829
AluI AGCT 8 cut(s) 47, 100, 245, 307, 602, 632, 811, 829
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 5 cut(s) 120, 201, 287, 370, 503
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 3 cut(s) 12, 374, 697
AoxI GGCC 2 cut(s) 357, 563
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 2 cut(s) 177, 307
ApoI RAATTY 3 cut(s) 12, 719, 977
AsiGI ACCGGT 1 cut(s) 171
AspLEI GCGC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 149, 161
BaeGI GKGCMC 1 cut(s) 451
BalI TGGCCA 1 cut(s) 359
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 70
BbsI GAAGAC 3 cut(s) 148, 480, 889
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 2 cut(s) 164, 294
BccI CCATC 3 cut(s) 295, 368, 590
BceAI ACGGC 2 cut(s) 84, 578
BciT130I CCWGG 1 cut(s) 27
BciVI GTATCC 1 cut(s) 246
BclI TGATCA 1 cut(s) 1024
BcoDI GTCTC 5 cut(s) 120, 201, 287, 370, 503
BfmI CTRYAG 2 cut(s) 276, 930
BfoI RGCGCY 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 937
BfuI GTATCC 1 cut(s) 246
BisI GCNGC 3 cut(s) 178, 308, 566
BlsI GCNGC 3 cut(s) 179, 309, 567
Bme1390I CCNGG 1 cut(s) 27
BmeRI GACNNNNNGTC 1 cut(s) 193
BmiI GGNNCC 5 cut(s) 6, 72, 112, 758, 909
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 1 cut(s) 958
BpiI GAAGAC 3 cut(s) 148, 480, 889
Bpu10I CCTNAGC 1 cut(s) 115
BsaAI YACGTR 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 25, 760
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 171
Bse1I ACTGG 1 cut(s) 854
Bse3DI GCAATG 2 cut(s) 170, 747
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 25, 760
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 2 cut(s) 170, 747
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 854
BseRI GAGGAG 1 cut(s) 902
BseSI GKGCMC 1 cut(s) 451
BseX3I CGGCCG 1 cut(s) 563
BseXI GCAGC 2 cut(s) 164, 294
Bsh1285I CGRYCG 1 cut(s) 566
BshFI GGCC 2 cut(s) 359, 565
BshNI GGYRCC 1 cut(s) 70
BshTI ACCGGT 1 cut(s) 171
BsiEI CGRYCG 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 451
BsiSI CCGG 1 cut(s) 172
BsiWI CGTACG 1 cut(s) 548
BslFI GGGAC 1 cut(s) 803
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 5 cut(s) 120, 201, 287, 370, 503
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 803
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 2 cut(s) 359, 565
Bsp1286I GDGCHC 1 cut(s) 451
Bsp143I GATC 4 cut(s) 4, 366, 689, 1024
BspACI CCGC 3 cut(s) 161, 566, 996
BspANI GGCC 2 cut(s) 359, 565
BspCNI CTCAG 1 cut(s) 119
BspLI GGNNCC 5 cut(s) 6, 72, 112, 758, 909
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 1 cut(s) 937
BspPI GGATC 3 cut(s) 12, 374, 697
BspQI GCTCTTC 2 cut(s) 380, 801
BspT107I GGYRCC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 170, 747
BsrFI RCCGGY 1 cut(s) 171
BsrI ACTGG 1 cut(s) 854
BssAI RCCGGY 1 cut(s) 171
BssECI CCNNGG 2 cut(s) 25, 760
BssMI GATC 4 cut(s) 4, 366, 689, 1024
BssNAI GTATAC 1 cut(s) 664
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 760
Bst1107I GTATAC 1 cut(s) 664
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 794
Bst6I CTCTTC 2 cut(s) 380, 801
BstACI GRCGYC 1 cut(s) 71
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 3 cut(s) 243, 634, 638
BstDEI CTNAG 3 cut(s) 48, 106, 115
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 4 cut(s) 7, 369, 692, 1027
BstMAI GTCTC 5 cut(s) 120, 201, 287, 370, 503
BstMBI GATC 4 cut(s) 4, 366, 689, 1024
BstMCI CGRYCG 1 cut(s) 566
BstMWI GCNNNNNNNGC 1 cut(s) 565
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 1 cut(s) 25
BstSFI CTRYAG 2 cut(s) 276, 930
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 2 cut(s) 164, 294
BstV2I GAAGAC 3 cut(s) 148, 480, 889
BstX2I RGATCY 2 cut(s) 4, 366
BstXI CCANNNNNNTGG 1 cut(s) 576
BstYI RGATCY 2 cut(s) 4, 366
BstZ17I GTATAC 1 cut(s) 664
BstZI CGGCCG 1 cut(s) 563
BsuI GTATCC 1 cut(s) 246
BsuRI GGCC 2 cut(s) 359, 565
BveI ACCTGC 1 cut(s) 937
Cac8I GCNNGC 3 cut(s) 243, 634, 638
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 171
Csp6I GTAC 3 cut(s) 549, 586, 992
CspAI ACCGGT 1 cut(s) 171
CviAII CATG 3 cut(s) 426, 667, 749
CviQI GTAC 3 cut(s) 549, 586, 992
DdeI CTNAG 3 cut(s) 48, 106, 115
DinI GGCGCC 1 cut(s) 72
DpnI GATC 4 cut(s) 6, 368, 691, 1026
DpnII GATC 4 cut(s) 4, 366, 689, 1024
DraI TTTAAA 1 cut(s) 976
DriI GACNNNNNGTC 1 cut(s) 193
EaeI YGGCCR 2 cut(s) 357, 563
EagI CGGCCG 1 cut(s) 563
Eam1104I CTCTTC 2 cut(s) 380, 801
Eam1105I GACNNNNNGTC 1 cut(s) 193
EarI CTCTTC 2 cut(s) 380, 801
EclXI CGGCCG 1 cut(s) 563
Eco130I CCWWGG 1 cut(s) 760
Eco32I GATATC 1 cut(s) 937
Eco52I CGGCCG 1 cut(s) 563
Eco57I CTGAAG 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 25
EcoRV GATATC 1 cut(s) 937
EcoT14I CCWWGG 1 cut(s) 760
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 760
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 3 cut(s) 429, 670, 752
FalI AAGNNNNNCTT 2 cut(s) 818, 850
FaqI GGGAC 1 cut(s) 803
FatI CATG 3 cut(s) 425, 666, 748
FauI CCCGC 2 cut(s) 168, 1003
FbaI TGATCA 1 cut(s) 1024
FblI GTMKAC 1 cut(s) 663
Fnu4HI GCNGC 3 cut(s) 178, 308, 566
Fsp4HI GCNGC 3 cut(s) 178, 308, 566
GlaI GCGC 1 cut(s) 72
GluI GCNGC 3 cut(s) 178, 308, 566
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 2 cut(s) 359, 565
HapII CCGG 1 cut(s) 172
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 3 cut(s) 429, 670, 752
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HinfI GANTC 4 cut(s) 154, 496, 704, 843
HpaII CCGG 1 cut(s) 172
HphI GGTGA 2 cut(s) 149, 161
Hpy166II GTNNAC 3 cut(s) 449, 664, 797
Hpy188I TCNGA 3 cut(s) 495, 703, 969
Hpy188III TCNNGA 6 cut(s) 8, 94, 108, 364, 464, 522
Hpy8I GTNNAC 3 cut(s) 449, 664, 797
HpyAV CCTTC 2 cut(s) 779, 854
HpyCH4III ACNGT 1 cut(s) 794
HpyCH4IV ACGT 3 cut(s) 194, 445, 547
HpyCH4V TGCA 4 cut(s) 449, 514, 745, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 565
HpyF3I CTNAG 3 cut(s) 48, 106, 115
HpySE526I ACGT 3 cut(s) 194, 445, 547
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 3 cut(s) 429, 670, 752
HspAI GCGC 1 cut(s) 71
KasI GGCGCC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 1024
Kzo9I GATC 4 cut(s) 4, 366, 689, 1024
LguI GCTCTTC 2 cut(s) 380, 801
LmnI GCTCC 4 cut(s) 110, 304, 599, 756
Lsp1109I GCAGC 2 cut(s) 164, 294
LweI GCATC 1 cut(s) 958
MaeII ACGT 3 cut(s) 194, 445, 547
MaeIII GTNAC 2 cut(s) 167, 267
MalI GATC 4 cut(s) 6, 368, 691, 1026
MboI GATC 4 cut(s) 4, 366, 689, 1024
MboII GAAGA 7 cut(s) 153, 397, 485, 818, 852, 889, 947
MfeI CAATTG 1 cut(s) 398
MflI RGATCY 2 cut(s) 4, 366
MhlI GDGCHC 1 cut(s) 451
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 7 cut(s) 12, 282, 352, 398, 589, 719, 977
MluNI TGGCCA 1 cut(s) 359
Mly113I GGCGCC 1 cut(s) 71
MlyI GAGTC 2 cut(s) 505, 698
MnlI CCTC 5 cut(s) 101, 178, 880, 883, 950
Mox20I TGGCCA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 359
MseI TTAA 3 cut(s) 42, 351, 975
MslI CAYNNNNRTG 1 cut(s) 987
Msp20I TGGCCA 1 cut(s) 359
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 1 cut(s) 27
MunI CAATTG 1 cut(s) 398
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 565
NarI GGCGCC 1 cut(s) 71
NdeII GATC 4 cut(s) 4, 366, 689, 1024
NlaIII CATG 3 cut(s) 429, 670, 752
NlaIV GGNNCC 5 cut(s) 6, 72, 112, 758, 909
NmuCI GTSAC 1 cut(s) 167
NspI RCATGY 1 cut(s) 752
PciSI GCTCTTC 2 cut(s) 380, 801
PctI GAATGC 1 cut(s) 514
PfeI GAWTC 2 cut(s) 154, 843
Pfl23II CGTACG 1 cut(s) 548
PinAI ACCGGT 1 cut(s) 171
PkrI GCNGC 3 cut(s) 179, 309, 567
PleI GAGTC 2 cut(s) 504, 698
PluTI GGCGCC 1 cut(s) 74
PpsI GAGTC 2 cut(s) 504, 698
Ppu21I YACGTR 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspLI CGTACG 1 cut(s) 548
PspN4I GGNNCC 5 cut(s) 6, 72, 112, 758, 909
PstI CTGCAG 1 cut(s) 934
PsuI RGATCY 2 cut(s) 4, 366
RsaI GTAC 3 cut(s) 550, 587, 993
RsaNI GTAC 3 cut(s) 549, 586, 992
RseI CAYNNNNRTG 1 cut(s) 987
SapI GCTCTTC 2 cut(s) 380, 801
SaqAI TTAA 3 cut(s) 42, 351, 975
SatI GCNGC 3 cut(s) 178, 308, 566
Sau3AI GATC 4 cut(s) 4, 366, 689, 1024
SchI GAGTC 2 cut(s) 505, 698
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 451
SfaNI GCATC 1 cut(s) 958
SfcI CTRYAG 2 cut(s) 276, 930
SfoI GGCGCC 1 cut(s) 72
SgrAI CRCCGGYG 1 cut(s) 171
SmiMI CAYNNNNRTG 1 cut(s) 987
Sse9I AATT 7 cut(s) 12, 282, 352, 398, 589, 719, 977
SsiI CCGC 3 cut(s) 161, 566, 996
SspDI GGCGCC 1 cut(s) 70
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 760
TaaI ACNGT 1 cut(s) 794
TaiI ACGT 3 cut(s) 197, 448, 550
TaqI TCGA 1 cut(s) 317
TasI AATT 7 cut(s) 12, 282, 352, 398, 589, 719, 977
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 568
TfiI GAWTC 2 cut(s) 154, 843
Tru1I TTAA 3 cut(s) 42, 351, 975
Tru9I TTAA 3 cut(s) 42, 351, 975
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 2 cut(s) 177, 307
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 3 cut(s) 507, 830, 991
TspGWI ACGGA 2 cut(s) 166, 466
VneI GTGCAC 1 cut(s) 447
XapI RAATTY 3 cut(s) 12, 719, 977
XceI RCATGY 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.