pycom10g25730

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000204
Physical Location & Seq
Reverse (-)
27264443 .. 27265057
615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25730.1

Sequence Viewer

Length: 615 bp
ATGTTAGCCTTTACAAAAATTTGGAGGTGCCAAAGTCTAACTAGTACTACAAAGCCCGGTTGGTTTTCAGTAAGAAATTATCGAAAAAAGCCGATTCCGGTGAAATGGGAAAAGCCCAAGATTGGATGGACCAAGTTGAACTTTGATGGATCTTCAAAAGGCAAAACAGGAAAAGCTAGCATTGGAGGGGTCTTTAGGAACCACAAGGCAGAATTCTTGCTAGGGTTTGCTGAGTCCATTGGAGAAGCCAACAGCACCATTGCTGAGTTGGCAGCCCTAAAAAGAGGCCTCGAGTTGGTGTTAGAAAATGGCTTTAGAGACGTGTGGCTTGAAGGAGATGCCAAGGCATTGCTCGATATTATTGTGAAAAGAAAGAAAGATTTTAAATGTGTAGAAGTTCAAAGGCAAGTTAGCGATGTGAATTCGATTTTGCCAAAACTCAACGGTTGCATTGTGAGTCACGTTTATAGGGAAGGCAATCGAGCCGCAGATAAATTTGCTCAAATTGGACATCAGCTAGAGGAGCCTAGGATCTGGTGGAACGTTCCTCCTGATGAAGTTGTAGCCATTGTGCATGAAGATGCAGAAGGCAAGATTGTCCTCCGCAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

205

Amino Acids

23.09

Weight (kDa)

9.68

Isoelectric Point (pI)

21.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 47 - 168 3e-28 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 27
AciI CCGC 2 cut(s) 486, 604
AclI AACGTT 1 cut(s) 543
AclWI GGATC 2 cut(s) 157, 539
AcsI RAATTY 4 cut(s) 18, 212, 421, 494
AfaI GTAC 1 cut(s) 46
AfiI CCNNNNNNNGG 2 cut(s) 122, 295
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 4 cut(s) 139, 156, 332, 401
AhlI ACTAGT 1 cut(s) 41
AjiI CACGTC 1 cut(s) 322
AluBI AGCT 2 cut(s) 176, 517
AluI AGCT 2 cut(s) 176, 517
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 2 cut(s) 157, 539
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 4 cut(s) 18, 212, 421, 494
AspA2I CCTAGG 1 cut(s) 527
AspS9I GGNCC 1 cut(s) 129
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 112
AsuNHI GCTAGC 1 cut(s) 176
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 1 cut(s) 129
AvrII CCTAGG 1 cut(s) 527
BanI GGYRCC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 284
BccI CCATC 2 cut(s) 120, 140
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 312
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 5 cut(s) 42, 177, 221, 518, 528
BisI GCNGC 2 cut(s) 273, 486
BlnI CCTAGG 1 cut(s) 527
BlsI GCNGC 2 cut(s) 274, 487
BmcAI AGTACT 1 cut(s) 46
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 1 cut(s) 129
BmeT110I CYCGRG 1 cut(s) 290
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 3 cut(s) 29, 200, 525
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 2 cut(s) 328, 571
BmtI GCTAGC 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 342, 527
BsaWI WCCGGW 1 cut(s) 97
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 295
Bse3DI GCAATG 2 cut(s) 258, 347
BseDI CCNNGG 2 cut(s) 342, 527
BseGI GGATG 1 cut(s) 131
BseLI CCNNNNNNNGG 2 cut(s) 122, 295
BseMI GCAATG 2 cut(s) 258, 347
BseMII CTCAG 2 cut(s) 222, 255
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 1 cut(s) 284
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 27
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 2 cut(s) 57, 98
BslI CCNNNNNNNGG 2 cut(s) 122, 295
BsmAI GTCTC 1 cut(s) 312
BsmBI CGTCTC 1 cut(s) 312
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 290
Bsp143I GATC 2 cut(s) 149, 531
BspACI CCGC 2 cut(s) 486, 604
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 2 cut(s) 223, 256
BspLI GGNNCC 3 cut(s) 29, 200, 525
BspOI GCTAGC 1 cut(s) 180
BspPI GGATC 2 cut(s) 157, 539
BspT107I GGYRCC 1 cut(s) 27
BsrDI GCAATG 2 cut(s) 258, 347
BssECI CCNNGG 2 cut(s) 342, 527
BssMI GATC 2 cut(s) 149, 531
BssT1I CCWWGG 2 cut(s) 342, 527
Bst4CI ACNGT 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 2 cut(s) 231, 264
BstF5I GGATG 1 cut(s) 131
BstKTI GATC 2 cut(s) 152, 534
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 2 cut(s) 149, 531
BstMWI GCNNNNNNNGC 2 cut(s) 269, 523
BstSCI CCNGG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 284
BstX2I RGATCY 2 cut(s) 149, 531
BstYI RGATCY 2 cut(s) 149, 531
BsuRI GGCC 1 cut(s) 288
BtgZI GCGATG 1 cut(s) 429
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 1 cut(s) 131
Cac8I GCNNGC 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 129
Csp6I GTAC 1 cut(s) 45
CviAII CATG 1 cut(s) 575
CviQI GTAC 1 cut(s) 45
DdeI CTNAG 2 cut(s) 231, 264
DpnI GATC 2 cut(s) 151, 533
DpnII GATC 2 cut(s) 149, 531
DraI TTTAAA 1 cut(s) 385
Eco130I CCWWGG 2 cut(s) 342, 527
Eco147I AGGCCT 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 290
EcoRI GAATTC 2 cut(s) 212, 421
EcoT14I CCWWGG 2 cut(s) 342, 527
ErhI CCWWGG 2 cut(s) 342, 527
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 1 cut(s) 578
FaiI YATR 2 cut(s) 468, 576
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FatI CATG 1 cut(s) 574
Fnu4HI GCNGC 2 cut(s) 273, 486
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 273, 486
FspBI CTAG 5 cut(s) 42, 177, 221, 518, 528
GluI GCNGC 2 cut(s) 273, 486
HaeIII GGCC 1 cut(s) 288
HapII CCGG 2 cut(s) 57, 98
Hin1II CATG 1 cut(s) 578
HinfI GANTC 3 cut(s) 94, 233, 457
HpaII CCGG 2 cut(s) 57, 98
HphI GGTGA 1 cut(s) 112
Hpy188III TCNNGA 1 cut(s) 551
HpyAV CCTTC 3 cut(s) 326, 467, 581
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4IV ACGT 3 cut(s) 321, 462, 543
HpyCH4V TGCA 3 cut(s) 450, 574, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 523
HpyF3I CTNAG 2 cut(s) 231, 264
HpySE526I ACGT 3 cut(s) 321, 462, 543
Hsp92II CATG 1 cut(s) 578
Kzo9I GATC 2 cut(s) 149, 531
LmnI GCTCC 1 cut(s) 523
LpnPI CCDG 5 cut(s) 70, 111, 153, 520, 564
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 2 cut(s) 328, 571
MaeI CTAG 5 cut(s) 42, 177, 221, 518, 528
MaeII ACGT 3 cut(s) 321, 462, 543
MaeIII GTNAC 1 cut(s) 458
MalI GATC 2 cut(s) 151, 533
MboI GATC 2 cut(s) 149, 531
MboII GAAGA 2 cut(s) 144, 590
MflI RGATCY 2 cut(s) 149, 531
MluCI AATT 6 cut(s) 18, 76, 212, 421, 494, 504
MlyI GAGTC 2 cut(s) 242, 466
MnlI CCTC 7 cut(s) 18, 179, 278, 299, 514, 558, 611
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 579
MspI CCGG 2 cut(s) 57, 98
MspR9I CCNGG 1 cut(s) 57
MwoI GCNNNNNNNGC 2 cut(s) 269, 523
NciI CCSGG 1 cut(s) 57
NdeII GATC 2 cut(s) 149, 531
NheI GCTAGC 1 cut(s) 176
NlaIII CATG 1 cut(s) 578
NlaIV GGNNCC 3 cut(s) 29, 200, 525
NmuCI GTSAC 1 cut(s) 458
PaeR7I CTCGAG 1 cut(s) 290
PceI AGGCCT 1 cut(s) 288
PfeI GAWTC 1 cut(s) 94
PkrI GCNGC 2 cut(s) 274, 487
PleI GAGTC 2 cut(s) 241, 465
PpsI GAGTC 2 cut(s) 241, 465
Psp1406I AACGTT 1 cut(s) 543
PspN4I GGNNCC 3 cut(s) 29, 200, 525
PspPI GGNCC 1 cut(s) 129
PspXI VCTCGAGB 1 cut(s) 290
PsuI RGATCY 2 cut(s) 149, 531
RsaI GTAC 1 cut(s) 46
RsaNI GTAC 1 cut(s) 45
RseI CAYNNNNRTG 1 cut(s) 579
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 273, 486
Sau3AI GATC 2 cut(s) 149, 531
Sau96I GGNCC 1 cut(s) 129
ScaI AGTACT 1 cut(s) 46
SchI GAGTC 2 cut(s) 242, 466
ScrFI CCNGG 1 cut(s) 57
SetI ASST 6 cut(s) 29, 178, 324, 465, 519, 546
SfaNI GCATC 2 cut(s) 328, 571
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 1 cut(s) 129
SlaI CTCGAG 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 6 cut(s) 18, 76, 212, 421, 494, 504
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 2 cut(s) 486, 604
SspMI CTAG 5 cut(s) 42, 177, 221, 518, 528
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 2 cut(s) 342, 527
TaaI ACNGT 1 cut(s) 446
TaiI ACGT 3 cut(s) 324, 465, 546
TaqI TCGA 5 cut(s) 82, 291, 354, 425, 481
TasI AATT 6 cut(s) 18, 76, 212, 421, 494, 504
TatI WGTACW 1 cut(s) 44
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 1 cut(s) 94
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 1 cut(s) 272
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 2 cut(s) 570, 591
VpaK11BI GGWCC 1 cut(s) 129
XapI RAATTY 4 cut(s) 18, 212, 421, 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 290
XmaJI CCTAGG 1 cut(s) 527
XspI CTAG 5 cut(s) 42, 177, 221, 518, 528
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.