Rh5BG044700

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000204
Physical Location & Seq
Forward (+)
3544377 .. 3546716
2340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG044700.1

Sequence Viewer

Length: 630 bp
ATGAGAAAGTGCAGGACAATGTTGGCCTTCGCTAAGATTTCAAGGTTCGAAAAACTAATACACCACCCTGCAGTTTGTCAATTGATCCGGAGTTTGCACAAGGAGTCAATTCCGGTAGCATGGGAGAAACCAAAAATTGGTTGGACTAAGCTGAACTTTGATGGATCATCCAAGGGCAAAGCAGAAAAGGCAAGCATTGGAGGGCTATTTAGAAATCACAAGGCAGAGTTTTTACTTGGATATGCTGAATCCATAGGAAGAGCAAACAGCGTCATTGCAGAACTTGCTGCACTAAGAAGAGGCCTTGAGTTGGTTTTGGAAAATGGTTGGAGTGATGTCTGGCTCGAAGGTGATGCCAAGACATTGCTTCATATCATTGCAAAAAGAAAACAGGTTAGATGTGCAGAAGCACAGAGGCATGTTTGTGAGATAAACTCAATTATTCCAGAACTTAACAACTGTGTTTTGACTCACATTTATAGAGAAGGTAACCGAGCTGCTGATAAATTTGCTAAAATGGGGCATTGGTATCAGAAGCCTCAAATTTGGGAGCACATTCCTCCTCATCAAGTGTTGTCCATCATGCATGAAGATGCTGAGGGCAAGGTTCTTTTCCGTAAAATAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

209

Amino Acids

23.93

Weight (kDa)

9.65

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 52 - 173 9.5e-29 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 87
AclWI GGATC 2 cut(s) 79, 172
AcsI RAATTY 2 cut(s) 506, 543
AfiI CCNNNNNNNGG 2 cut(s) 137, 310
AgsI TTSAA 1 cut(s) 42
AluBI AGCT 2 cut(s) 151, 497
AluI AGCT 2 cut(s) 151, 497
Alw21I GWGCWC 1 cut(s) 555
AlwI GGATC 2 cut(s) 79, 172
Aor13HI TCCGGA 1 cut(s) 87
AoxI GGCC 2 cut(s) 24, 301
ApeKI GCWGC 2 cut(s) 287, 497
ApoI RAATTY 2 cut(s) 506, 543
AsuHPI GGTGA 1 cut(s) 362
AsuII TTCGAA 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 555
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 2 cut(s) 274, 484
BccI CCATC 2 cut(s) 155, 587
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BfmI CTRYAG 1 cut(s) 69
BisI GCNGC 2 cut(s) 288, 498
BlsI GCNGC 2 cut(s) 289, 499
BmsI GCATC 2 cut(s) 343, 583
BplI GAGNNNNNCTC 2 cut(s) 419, 451
Bpu10I CCTNAGC 1 cut(s) 597
Bpu14I TTCGAA 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 2 cut(s) 87, 112
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 310
Bse3DI GCAATG 3 cut(s) 273, 362, 375
BseAI TCCGGA 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 137, 310
BseMI GCAATG 3 cut(s) 273, 362, 375
BseMII CTCAG 1 cut(s) 588
BseRI GAGGAG 1 cut(s) 552
BseXI GCAGC 2 cut(s) 274, 484
BsgI GTGCAG 3 cut(s) 31, 273, 423
BshFI GGCC 2 cut(s) 26, 303
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 2 cut(s) 88, 113
BslI CCNNNNNNNGG 2 cut(s) 137, 310
BsnI GGCC 2 cut(s) 26, 303
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 555
Bsp13I TCCGGA 1 cut(s) 87
Bsp143I GATC 2 cut(s) 84, 164
BspANI GGCC 2 cut(s) 26, 303
BspCNI CTCAG 1 cut(s) 589
BspEI TCCGGA 1 cut(s) 87
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 2 cut(s) 79, 172
BspQI GCTCTTC 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 48
BsrDI GCAATG 3 cut(s) 273, 362, 375
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 2 cut(s) 84, 164
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 2 cut(s) 253, 292
BstAPI GCANNNNNTGC 1 cut(s) 284
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 4 cut(s) 33, 147, 293, 597
BstEII GGTNACC 1 cut(s) 488
BstF5I GGATG 1 cut(s) 167
BstKTI GATC 2 cut(s) 87, 167
BstMBI GATC 2 cut(s) 84, 164
BstMWI GCNNNNNNNGC 2 cut(s) 188, 284
BstNSI RCATGY 1 cut(s) 422
BstPI GGTNACC 1 cut(s) 488
BstSFI CTRYAG 1 cut(s) 69
BstV1I GCAGC 2 cut(s) 274, 484
BsuRI GGCC 2 cut(s) 26, 303
BtsCI GGATG 1 cut(s) 167
Cac8I GCNNGC 1 cut(s) 193
CseI GACGC 1 cut(s) 259
CviAII CATG 4 cut(s) 120, 419, 583, 587
CviJI RGCY 7 cut(s) 26, 151, 205, 303, 343, 497, 538
CviKI_1 RGCY 7 cut(s) 26, 151, 205, 303, 343, 497, 538
DdeI CTNAG 4 cut(s) 33, 147, 293, 597
DpnI GATC 2 cut(s) 86, 166
DpnII GATC 2 cut(s) 84, 164
Eam1104I CTCTTC 2 cut(s) 253, 292
EarI CTCTTC 2 cut(s) 253, 292
Eco130I CCWWGG 1 cut(s) 171
Eco147I AGGCCT 1 cut(s) 303
Eco91I GGTNACC 1 cut(s) 488
EcoO65I GGTNACC 1 cut(s) 488
EcoT14I CCWWGG 1 cut(s) 171
EcoT22I ATGCAT 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 4 cut(s) 123, 422, 586, 590
FaiI YATR 8 cut(s) 121, 243, 254, 372, 420, 480, 584, 588
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FatI CATG 4 cut(s) 119, 418, 582, 586
Fnu4HI GCNGC 2 cut(s) 288, 498
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 2 cut(s) 288, 498
GluI GCNGC 2 cut(s) 288, 498
HaeIII GGCC 2 cut(s) 26, 303
HapII CCGG 2 cut(s) 88, 113
HgaI GACGC 1 cut(s) 259
Hin1II CATG 4 cut(s) 123, 422, 586, 590
HinfI GANTC 3 cut(s) 104, 248, 469
HpaII CCGG 2 cut(s) 88, 113
HphI GGTGA 1 cut(s) 362
Hpy188I TCNGA 1 cut(s) 534
Hpy188III TCNNGA 2 cut(s) 88, 446
HpyAV CCTTC 3 cut(s) 37, 341, 479
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4V TGCA 8 cut(s) 12, 71, 97, 278, 290, 380, 404, 586
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 284
HpyF3I CTNAG 4 cut(s) 33, 147, 293, 597
Hsp92II CATG 4 cut(s) 123, 422, 586, 590
Kpn2I TCCGGA 1 cut(s) 87
Kzo9I GATC 2 cut(s) 84, 164
LguI GCTCTTC 1 cut(s) 253
LmnI GCTCC 1 cut(s) 550
LpnPI CCDG 6 cut(s) 81, 101, 126, 325, 377, 459
Lsp1109I GCAGC 2 cut(s) 274, 484
LweI GCATC 2 cut(s) 343, 583
MaeIII GTNAC 1 cut(s) 488
MalI GATC 2 cut(s) 86, 166
MboI GATC 2 cut(s) 84, 164
MboII GAAGA 3 cut(s) 270, 309, 602
MfeI CAATTG 1 cut(s) 80
MhlI GDGCHC 1 cut(s) 555
MluCI AATT 6 cut(s) 80, 108, 135, 438, 506, 543
MlyI GAGTC 2 cut(s) 113, 463
MmeI TCCRAC 2 cut(s) 122, 308
MnlI CCTC 7 cut(s) 194, 293, 408, 549, 570, 573, 592
Mph1103I ATGCAT 1 cut(s) 588
MroI TCCGGA 1 cut(s) 87
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 2 cut(s) 423, 591
MspI CCGG 2 cut(s) 88, 113
MunI CAATTG 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 188, 284
NdeII GATC 2 cut(s) 84, 164
NlaIII CATG 4 cut(s) 123, 422, 586, 590
NsiI ATGCAT 1 cut(s) 588
NspI RCATGY 1 cut(s) 422
NspV TTCGAA 1 cut(s) 48
PceI AGGCCT 1 cut(s) 303
PciSI GCTCTTC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 137
PkrI GCNGC 2 cut(s) 289, 499
PleI GAGTC 2 cut(s) 112, 463
PpsI GAGTC 2 cut(s) 112, 463
PspEI GGTNACC 1 cut(s) 488
PstI CTGCAG 1 cut(s) 73
RseI CAYNNNNRTG 2 cut(s) 423, 591
SapI GCTCTTC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 288, 498
Sau3AI GATC 2 cut(s) 84, 164
SchI GAGTC 2 cut(s) 113, 463
SduI GDGCHC 1 cut(s) 555
SetI ASST 7 cut(s) 47, 153, 352, 396, 490, 499, 609
SfaNI GCATC 2 cut(s) 343, 583
SfcI CTRYAG 1 cut(s) 69
SfuI TTCGAA 1 cut(s) 48
SmiMI CAYNNNNRTG 2 cut(s) 423, 591
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 6 cut(s) 80, 108, 135, 438, 506, 543
SseBI AGGCCT 1 cut(s) 303
StuI AGGCCT 1 cut(s) 303
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 1 cut(s) 461
TaqI TCGA 2 cut(s) 48, 345
TasI AATT 6 cut(s) 80, 108, 135, 438, 506, 543
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TseI GCWGC 2 cut(s) 287, 497
TspDTI ATGAA 2 cut(s) 359, 603
TspGWI ACGGA 1 cut(s) 605
Van91I CCANNNNNTGG 1 cut(s) 137
XapI RAATTY 2 cut(s) 506, 543
XceI RCATGY 1 cut(s) 422
XcmI CCANNNNNNNNNTGG 1 cut(s) 138
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.