pycom08g21670

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000204
Physical Location & Seq
Reverse (-)
21090924 .. 21091493
570 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21670.1

Sequence Viewer

Length: 570 bp
ATGTCTAAGTGTAAGGAAGCACAACGGGGAAGCAGAACAACCATAAATGCAATTGAAGGAACCAATAAACTGGAATTTGCAGGAAGAAAGGTGACATGGAAAATACCACAGTTTGGGACACTTAAGGTGAATTGTGATGCAGCTTGGAATCCACAATCACACAAAGGTGGTGTGGGATGGGTTATAAGGAACTTTGCCGGCCTGCTGCACTCTGCAGGAGGATGGGAAAATCTGCACCGTTCCGTTGAAATTGTTGAAGCTACCGCGTTGAGGGCAGCGCTGCAGGAGTGTGCAAAGAAAGACATCCATTCTATTGAGATTGAAACTGATGCAAAGACAATCATGCAGATGATCAATAAGGAAACTGGAACAGAAGCATCTTTGGAGGATATCATTCATGACATCTGGAAACTCGCTCAAACATTCCAAAATGCAACGTTGACGTATGCCCATCGGGAGTGCAACCACGCTGCTCGTGCTGTTGCCTCTTATGTCTCTAAGGATGGAAGTGTCCATTTATGGTATTGTATTGGCCCACATTTTATTTTCAATATCATAGCGAAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

190

Amino Acids

21.08

Weight (kDa)

8.59

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 44 - 164 5.4e-26 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 113
AccII CGCG 1 cut(s) 266
AciI CCGC 1 cut(s) 264
AclI AACGTT 1 cut(s) 437
AcsI RAATTY 1 cut(s) 74
AfeI AGCGCT 1 cut(s) 279
AfiI CCNNNNNNNGG 2 cut(s) 113, 270
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 5 cut(s) 56, 248, 257, 323, 550
AleI CACNNNNGTG 1 cut(s) 165
AluBI AGCT 2 cut(s) 143, 260
AluI AGCT 2 cut(s) 143, 260
Alw26I GTCTC 1 cut(s) 499
Aor51HI AGCGCT 1 cut(s) 279
AoxI GGCC 2 cut(s) 199, 532
ApeKI GCWGC 5 cut(s) 140, 205, 275, 280, 470
ApoI RAATTY 1 cut(s) 74
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 2 cut(s) 103, 139
BauI CACGAG 1 cut(s) 474
BbvI GCAGC 5 cut(s) 152, 192, 267, 287, 457
BccI CCATC 4 cut(s) 171, 216, 459, 497
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 499
BfmI CTRYAG 2 cut(s) 213, 281
BfoI RGCGCY 1 cut(s) 281
BfrI CTTAAG 1 cut(s) 122
BisI GCNGC 5 cut(s) 141, 206, 276, 281, 471
BlsI GCNGC 5 cut(s) 142, 207, 277, 282, 472
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 1 cut(s) 61
BmsI GCATC 3 cut(s) 127, 319, 386
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 270
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 2 cut(s) 75, 370
BseGI GGATG 4 cut(s) 182, 227, 303, 508
BseLI CCNNNNNNNGG 2 cut(s) 113, 270
BseNI ACTGG 2 cut(s) 75, 370
BseXI GCAGC 5 cut(s) 152, 192, 267, 287, 457
BsgI GTGCAG 2 cut(s) 191, 218
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 2 cut(s) 201, 534
BsiSI CCGG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 2 cut(s) 113, 270
BsmAI GTCTC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 2 cut(s) 201, 534
Bsp143I GATC 1 cut(s) 351
BspACI CCGC 1 cut(s) 264
BspANI GGCC 2 cut(s) 201, 534
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 1 cut(s) 61
BspMAI CTGCAG 2 cut(s) 217, 285
BspTI CTTAAG 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 2 cut(s) 75, 370
BssAI RCCGGY 1 cut(s) 197
BssMI GATC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 474
Bst4CI ACNGT 2 cut(s) 111, 239
BstAFI CTTAAG 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 199, 203
BstDEI CTNAG 2 cut(s) 6, 498
BstF5I GGATG 4 cut(s) 182, 227, 303, 508
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 1 cut(s) 354
BstMAI GTCTC 1 cut(s) 499
BstMBI GATC 1 cut(s) 351
BstMWI GCNNNNNNNGC 2 cut(s) 272, 476
BstSFI CTRYAG 2 cut(s) 213, 281
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 5 cut(s) 152, 192, 267, 287, 457
BstXI CCANNNNNNTGG 1 cut(s) 70
BsuRI GGCC 2 cut(s) 201, 534
BtsCI GGATG 4 cut(s) 182, 227, 303, 508
Cac8I GCNNGC 2 cut(s) 199, 203
CciI TCATGA 1 cut(s) 397
CfoI GCGC 1 cut(s) 280
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 533
CviAII CATG 3 cut(s) 96, 343, 398
CviJI RGCY 4 cut(s) 143, 201, 260, 534
CviKI_1 RGCY 4 cut(s) 143, 201, 260, 534
DdeI CTNAG 2 cut(s) 6, 498
DpnI GATC 1 cut(s) 353
DpnII GATC 1 cut(s) 351
Eco32I GATATC 1 cut(s) 391
Eco47III AGCGCT 1 cut(s) 279
EcoRV GATATC 1 cut(s) 391
FaeI CATG 3 cut(s) 99, 346, 401
FaiI YATR 9 cut(s) 44, 97, 185, 344, 399, 447, 492, 520, 557
FaqI GGGAC 1 cut(s) 130
FatI CATG 3 cut(s) 95, 342, 397
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 5 cut(s) 141, 206, 276, 281, 471
FokI GGATG 4 cut(s) 189, 234, 290, 515
Fsp4HI GCNGC 5 cut(s) 141, 206, 276, 281, 471
GlaI GCGC 1 cut(s) 279
GluI GCNGC 5 cut(s) 141, 206, 276, 281, 471
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 2 cut(s) 201, 534
HapII CCGG 1 cut(s) 198
HhaI GCGC 1 cut(s) 280
Hin1II CATG 3 cut(s) 99, 346, 401
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 1 cut(s) 441
HindII GTYRAC 1 cut(s) 441
HinfI GANTC 1 cut(s) 148
HpaII CCGG 1 cut(s) 198
HphI GGTGA 2 cut(s) 103, 139
Hpy166II GTNNAC 1 cut(s) 441
Hpy188III TCNNGA 3 cut(s) 398, 406, 455
Hpy8I GTNNAC 1 cut(s) 441
HpyAV CCTTC 1 cut(s) 50
HpyCH4III ACNGT 2 cut(s) 111, 239
HpyCH4IV ACGT 2 cut(s) 437, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 476
HpyF3I CTNAG 2 cut(s) 6, 498
HpySE526I ACGT 2 cut(s) 437, 443
Hsp92II CATG 3 cut(s) 99, 346, 401
HspAI GCGC 1 cut(s) 278
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 1 cut(s) 351
LpnPI CCDG 8 cut(s) 56, 66, 201, 211, 215, 269, 351, 391
Lsp1109I GCAGC 5 cut(s) 152, 192, 267, 287, 457
LweI GCATC 3 cut(s) 127, 319, 386
MaeII ACGT 2 cut(s) 437, 443
MaeIII GTNAC 1 cut(s) 91
MalI GATC 1 cut(s) 353
MboI GATC 1 cut(s) 351
MboII GAAGA 1 cut(s) 96
MfeI CAATTG 1 cut(s) 51
MluCI AATT 4 cut(s) 51, 74, 130, 249
MnlI CCTC 4 cut(s) 212, 264, 379, 496
MroNI GCCGGC 1 cut(s) 197
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 2 cut(s) 165, 347
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 1 cut(s) 198
MunI CAATTG 1 cut(s) 51
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 272, 476
NaeI GCCGGC 1 cut(s) 199
NdeII GATC 1 cut(s) 351
NgoMIV GCCGGC 1 cut(s) 197
NlaIII CATG 3 cut(s) 99, 346, 401
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 91
OliI CACNNNNGTG 1 cut(s) 165
PagI TCATGA 1 cut(s) 397
PdiI GCCGGC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 113
PkrI GCNGC 5 cut(s) 142, 207, 277, 282, 472
PsiI TTATAA 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 437
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 1 cut(s) 533
PstI CTGCAG 2 cut(s) 217, 285
RseI CAYNNNNRTG 2 cut(s) 165, 347
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 5 cut(s) 141, 206, 276, 281, 471
Sau3AI GATC 1 cut(s) 351
Sau96I GGNCC 1 cut(s) 533
SetI ASST 7 cut(s) 93, 129, 145, 169, 262, 440, 446
SfaNI GCATC 3 cut(s) 127, 319, 386
SfcI CTRYAG 2 cut(s) 213, 281
SmiMI CAYNNNNRTG 2 cut(s) 165, 347
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 4 cut(s) 51, 74, 130, 249
SsiI CCGC 1 cut(s) 264
TaaI ACNGT 2 cut(s) 111, 239
TaiI ACGT 2 cut(s) 440, 446
TasI AATT 4 cut(s) 51, 74, 130, 249
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 5 cut(s) 140, 205, 275, 280, 470
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 386
TspGWI ACGGA 1 cut(s) 232
Van91I CCANNNNNTGG 1 cut(s) 113
Vha464I CTTAAG 1 cut(s) 122
XapI RAATTY 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.