pycom14g10440

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000204
Physical Location & Seq
Forward (+)
13111144 .. 13111767
624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g10440.1

Sequence Viewer

Length: 624 bp
ATGTTGGCGAATCTGGAAGTGCAGAAATTCTGCTACTTTCCACGGAGAAACCTGCTGCCCGGTGGAAGCTCTCAACAATCTAAAATATCCAATCGAAGGAGTGGGTCGCGGCCGGCGGCAAGAAGAGAGGAGCAGGTCACCGCGGAAAAGAATACAAGGAGGGAGGAGACGGTTTGGAGGAAGCCTGCAATTGGTTGTTTGAAGGTTAATTGTGTGGTGCATGGAGATCGAGAAGAGACGATGATTGGAGGTACGGGTTGGGTGATTAGAAACAGTGCTGGGTGTGGGTTGTTCCAAATGGGAGGGGGACGTGGTGGAGTGAGGGCCGCCTCCTGTTTAGCCATCGAAGCTGTGGCGGTGAAGCCGGTGAGGGAGGCCTTATGGGCTTGCGTTGAGAAGGGATACCAGCAAATAATTGTGGAGTGTGATTCCTTTAATCTCATCTCGATGCTTAATGGTAAAGAAGTATATGATGCTGCCATTGAAAGTTTAATCTTTGACATTCGAGAGCTTGTCAAACAATTGAGTGCTGTCGAGTATGCTTTCGTGACTAGAAACTGCAATAAGGCGGCTCACTTGATGGCCTCATACACTAATACTAAAGGGATCAGGATCCTCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

208

Amino Acids

22.87

Weight (kDa)

9.3

Isoelectric Point (pI)

55.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 79 - 197 8.9e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 60, 124
AccII CGCG 2 cut(s) 109, 143
AciI CCGC 7 cut(s) 109, 116, 141, 143, 327, 356, 569
AclWI GGATC 3 cut(s) 607, 614, 620
AcoI YGGCCR 1 cut(s) 110
AcsI RAATTY 1 cut(s) 26
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 2 cut(s) 96, 191
AgsI TTSAA 2 cut(s) 202, 485
AjiI CACGTC 1 cut(s) 311
AluBI AGCT 3 cut(s) 69, 350, 511
AluI AGCT 3 cut(s) 69, 350, 511
Alw26I GTCTC 2 cut(s) 161, 230
AlwI GGATC 3 cut(s) 607, 614, 620
AoxI GGCC 4 cut(s) 110, 324, 375, 582
ApeKI GCWGC 2 cut(s) 55, 476
ApoI RAATTY 1 cut(s) 26
AspS9I GGNCC 1 cut(s) 324
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 4 cut(s) 130, 274, 370, 379
BamHI GGATCC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 42, 463
BccI CCATC 2 cut(s) 350, 574
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciVI GTATCC 1 cut(s) 395
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 2 cut(s) 161, 230
BfaI CTAG 1 cut(s) 552
BfuAI ACCTGC 2 cut(s) 60, 124
BfuI GTATCC 1 cut(s) 395
BglI GCCNNNNNGGC 1 cut(s) 383
BisI GCNGC 6 cut(s) 56, 110, 117, 327, 477, 570
BlsI GCNGC 6 cut(s) 57, 111, 118, 328, 478, 571
Bme1390I CCNGG 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 324
BmiI GGNNCC 1 cut(s) 614
BmrFI CCNGG 1 cut(s) 60
BmsI GCATC 2 cut(s) 438, 463
BpuMI CCSGG 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 41, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 191
Bse118I RCCGGY 2 cut(s) 112, 364
Bse8I GATNNNNATC 1 cut(s) 611
BseDI CCNNGG 2 cut(s) 41, 141
BseJI GATNNNNATC 1 cut(s) 611
BseLI CCNNNNNNNGG 2 cut(s) 96, 191
BseRI GAGGAG 2 cut(s) 143, 179
BseX3I CGGCCG 1 cut(s) 110
BseXI GCAGC 2 cut(s) 42, 463
BseYI CCCAGC 1 cut(s) 278
BsgI GTGCAG 1 cut(s) 41
Bsh1236I CGCG 2 cut(s) 109, 143
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 4 cut(s) 112, 326, 377, 584
BsiEI CGRYCG 1 cut(s) 113
BsiSI CCGG 3 cut(s) 60, 113, 365
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 96, 191
BsmAI GTCTC 2 cut(s) 161, 230
BsmBI CGTCTC 2 cut(s) 161, 230
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 4 cut(s) 112, 326, 377, 584
Bsp143I GATC 3 cut(s) 226, 606, 612
BspACI CCGC 7 cut(s) 109, 116, 141, 143, 327, 356, 569
BspANI GGCC 4 cut(s) 112, 326, 377, 584
BspFNI CGCG 2 cut(s) 109, 143
BspLI GGNNCC 1 cut(s) 614
BspMI ACCTGC 2 cut(s) 60, 124
BspPI GGATC 3 cut(s) 607, 614, 620
BsrFI RCCGGY 2 cut(s) 112, 364
BssAI RCCGGY 2 cut(s) 112, 364
BssECI CCNNGG 2 cut(s) 41, 141
BssMI GATC 3 cut(s) 226, 606, 612
Bst4CI ACNGT 2 cut(s) 172, 275
Bst6I CTCTTC 2 cut(s) 118, 228
BstC8I GCNNGC 3 cut(s) 114, 186, 388
BstDSI CCRYGG 2 cut(s) 41, 141
BstEII GGTNACC 1 cut(s) 136
BstFNI CGCG 2 cut(s) 109, 143
BstKTI GATC 3 cut(s) 229, 609, 615
BstMAI GTCTC 2 cut(s) 161, 230
BstMBI GATC 3 cut(s) 226, 606, 612
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 2 cut(s) 347, 383
BstPI GGTNACC 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 58
BstUI CGCG 2 cut(s) 109, 143
BstV1I GCAGC 2 cut(s) 42, 463
BstX2I RGATCY 1 cut(s) 612
BstYI RGATCY 1 cut(s) 612
BstZI CGGCCG 1 cut(s) 110
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 4 cut(s) 112, 326, 377, 584
BtgI CCRYGG 2 cut(s) 41, 141
BtrI CACGTC 1 cut(s) 311
BtsIMutI CAGTG 1 cut(s) 280
BveI ACCTGC 2 cut(s) 60, 124
Cac8I GCNNGC 3 cut(s) 114, 186, 388
Cfr10I RCCGGY 2 cut(s) 112, 364
Cfr13I GGNCC 1 cut(s) 324
Cfr42I CCGCGG 1 cut(s) 144
Csp6I GTAC 1 cut(s) 252
CviAII CATG 1 cut(s) 221
CviQI GTAC 1 cut(s) 252
DpnI GATC 3 cut(s) 228, 608, 614
DpnII GATC 3 cut(s) 226, 606, 612
EaeI YGGCCR 1 cut(s) 110
EagI CGGCCG 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 118, 228
EarI CTCTTC 2 cut(s) 118, 228
EclXI CGGCCG 1 cut(s) 110
Eco147I AGGCCT 1 cut(s) 377
Eco52I CGGCCG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 136
Esp3I CGTCTC 2 cut(s) 161, 230
FaeI CATG 1 cut(s) 224
FaiI YATR 6 cut(s) 222, 382, 469, 471, 540, 589
FaqI GGGAC 1 cut(s) 321
FatI CATG 1 cut(s) 220
Fnu4HI GCNGC 6 cut(s) 56, 110, 117, 327, 477, 570
Fsp4HI GCNGC 6 cut(s) 56, 110, 117, 327, 477, 570
FspBI CTAG 1 cut(s) 552
GluI GCNGC 6 cut(s) 56, 110, 117, 327, 477, 570
GsaI CCCAGC 1 cut(s) 282
HaeIII GGCC 4 cut(s) 112, 326, 377, 584
HapII CCGG 3 cut(s) 60, 113, 365
Hin1II CATG 1 cut(s) 224
HinfI GANTC 2 cut(s) 10, 428
HpaII CCGG 3 cut(s) 60, 113, 365
HphI GGTGA 4 cut(s) 130, 274, 370, 379
Hpy188III TCNNGA 6 cut(s) 14, 230, 445, 506, 547, 610
HpyAV CCTTC 3 cut(s) 90, 196, 391
HpyCH4III ACNGT 2 cut(s) 172, 275
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 4 cut(s) 22, 188, 220, 561
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 383
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 1 cut(s) 224
KroI GCCGGC 1 cut(s) 112
KroNI GCCGGC 1 cut(s) 114
KspI CCGCGG 1 cut(s) 144
Kzo9I GATC 3 cut(s) 226, 606, 612
LmnI GCTCC 1 cut(s) 130
Lsp1109I GCAGC 2 cut(s) 42, 463
LweI GCATC 2 cut(s) 438, 463
MaeI CTAG 1 cut(s) 552
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 2 cut(s) 136, 547
MalI GATC 3 cut(s) 228, 608, 614
MboI GATC 3 cut(s) 226, 606, 612
MboII GAAGA 2 cut(s) 135, 245
MfeI CAATTG 2 cut(s) 189, 521
MflI RGATCY 1 cut(s) 612
MluCI AATT 5 cut(s) 26, 189, 208, 414, 521
MroNI GCCGGC 1 cut(s) 112
MseI TTAA 4 cut(s) 207, 435, 453, 491
MslI CAYNNNNRTG 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 3 cut(s) 60, 113, 365
MspR9I CCNGG 1 cut(s) 60
MunI CAATTG 2 cut(s) 189, 521
MvnI CGCG 2 cut(s) 109, 143
MwoI GCNNNNNNNGC 2 cut(s) 347, 383
NaeI GCCGGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 60
NdeII GATC 3 cut(s) 226, 606, 612
NgoMIV GCCGGC 1 cut(s) 112
NlaIII CATG 1 cut(s) 224
NlaIV GGNNCC 1 cut(s) 614
NmuCI GTSAC 2 cut(s) 136, 547
PceI AGGCCT 1 cut(s) 377
PdiI GCCGGC 1 cut(s) 114
PfeI GAWTC 2 cut(s) 10, 428
PkrI GCNGC 6 cut(s) 57, 111, 118, 328, 478, 571
PspEI GGTNACC 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 614
PspPI GGNCC 1 cut(s) 324
PsuI RGATCY 1 cut(s) 612
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 446
SacII CCGCGG 1 cut(s) 144
SaqAI TTAA 4 cut(s) 207, 435, 453, 491
SatI GCNGC 6 cut(s) 56, 110, 117, 327, 477, 570
Sau3AI GATC 3 cut(s) 226, 606, 612
Sau96I GGNCC 1 cut(s) 324
ScrFI CCNGG 1 cut(s) 60
SetI ASST 8 cut(s) 54, 71, 138, 207, 253, 313, 352, 513
SfaNI GCATC 2 cut(s) 438, 463
Sfr303I CCGCGG 1 cut(s) 144
SgrBI CCGCGG 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 5 cut(s) 26, 189, 208, 414, 521
SseBI AGGCCT 1 cut(s) 377
SsiI CCGC 7 cut(s) 109, 116, 141, 143, 327, 356, 569
SspMI CTAG 1 cut(s) 552
StuI AGGCCT 1 cut(s) 377
StyD4I CCNGG 1 cut(s) 58
TaaI ACNGT 2 cut(s) 172, 275
TaiI ACGT 1 cut(s) 313
TaqI TCGA 6 cut(s) 94, 229, 345, 446, 505, 534
TasI AATT 5 cut(s) 26, 189, 208, 414, 521
TauI GCSGC 4 cut(s) 112, 119, 329, 572
TfiI GAWTC 2 cut(s) 10, 428
Tru1I TTAA 4 cut(s) 207, 435, 453, 491
Tru9I TTAA 4 cut(s) 207, 435, 453, 491
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 2 cut(s) 136, 547
TseI GCWGC 2 cut(s) 55, 476
Tsp45I GTSAC 2 cut(s) 136, 547
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 1 cut(s) 280
XapI RAATTY 1 cut(s) 26
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.