pycom10g25740

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000204
Physical Location & Seq
Reverse (-)
27267371 .. 27267979
609 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25740.1

Sequence Viewer

Length: 609 bp
ATGTTAGCTTTCAATAAGATTTTGAGATTCGAAAAACTAATACAACAACGTCCGGTTTGCCTATTCCGGAGTTTGCACAAGGAGTCAATTCCGGTGTCATGGGAAAAACCGAAAATTGGCTGGACTAAACTGAACTTTGATGGATCTTCCAAACCCGAAGTTGGAAAGGCTAGCATTGGAGGGCTGTTCAGAAACCACAAGGCCGAGTTCCTACTTGGTTATGCTGAATCCATAGGAAAGGCCAACAGCATCATTGCGGAACTGGCTGCGCTCAGAAGAGGCCTTGAGTTGGTGTTGGAAAATGGTTGGAGCGACATGTGGCTCGAAGGAGACGCGAAGACATTGCTTCAGATTATCGCGCAGAGGAGGCAGATTCGATGCGTGGAGGCACAAAGGCAGATTAGTGACATAAACTTGATCATACCGGAGATTAACAACTGCGTCCTGACTCACGTTTATAGAGAAGGCAACAGGGCCGCTGATAAGTTTGCGAAATTGGGACATTGTTATGGAAAGCCTAAAATTTGGCAGCACATTCCTCCCGATGAAGTGTTATCCATCGTGCAAGAAGATGCCGAGGGTAAGATCGTTTTTCGTCGTAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

203

Amino Acids

23.08

Weight (kDa)

9.51

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 45 - 166 1.2e-26 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 335, 359
AccIII TCCGGA 1 cut(s) 66
AciI CCGC 2 cut(s) 257, 477
AclWI GGATC 1 cut(s) 151
AcsI RAATTY 1 cut(s) 522
AcuI CTGAAG 1 cut(s) 332
AfiI CCNNNNNNNGG 3 cut(s) 116, 161, 289
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 1 cut(s) 13
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 1 cut(s) 151
Aor13HI TCCGGA 1 cut(s) 66
AoxI GGCC 4 cut(s) 201, 240, 280, 474
ApeKI GCWGC 2 cut(s) 266, 529
ApoI RAATTY 1 cut(s) 522
AspLEI GCGC 2 cut(s) 271, 361
AspS9I GGNCC 1 cut(s) 474
AsuII TTCGAA 1 cut(s) 30
AsuNHI GCTAGC 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 344
BbvI GCAGC 2 cut(s) 253, 541
BccI CCATC 2 cut(s) 134, 566
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BclI TGATCA 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 1 cut(s) 171
BisI GCNGC 3 cut(s) 267, 477, 530
BlsI GCNGC 3 cut(s) 268, 478, 531
BmgT120I GGNCC 1 cut(s) 474
BmsI GCATC 3 cut(s) 258, 368, 562
BmtI GCTAGC 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 344
Bpu14I TTCGAA 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 576
BsaWI WCCGGW 4 cut(s) 52, 66, 91, 424
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 161, 289
Bse1I ACTGG 1 cut(s) 267
Bse3DI GCAATG 2 cut(s) 252, 341
BseAI TCCGGA 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 576
BseLI CCNNNNNNNGG 3 cut(s) 116, 161, 289
BseMI GCAATG 2 cut(s) 252, 341
BseMII CTCAG 1 cut(s) 286
BseNI ACTGG 1 cut(s) 267
BseRI GAGGAG 1 cut(s) 379
BseXI GCAGC 2 cut(s) 253, 541
Bsh1236I CGCG 2 cut(s) 335, 359
BshFI GGCC 4 cut(s) 203, 242, 282, 476
BsiSI CCGG 4 cut(s) 53, 67, 92, 425
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 3 cut(s) 116, 161, 289
BsmAI GTCTC 1 cut(s) 324
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 513
BsnI GGCC 4 cut(s) 203, 242, 282, 476
Bsp119I TTCGAA 1 cut(s) 30
Bsp13I TCCGGA 1 cut(s) 66
Bsp143I GATC 3 cut(s) 143, 417, 585
BspACI CCGC 2 cut(s) 257, 477
BspANI GGCC 4 cut(s) 203, 242, 282, 476
BspCNI CTCAG 1 cut(s) 285
BspEI TCCGGA 1 cut(s) 66
BspFNI CGCG 2 cut(s) 335, 359
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 30
BsrDI GCAATG 2 cut(s) 252, 341
BsrI ACTGG 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 3 cut(s) 143, 417, 585
Bst6I CTCTTC 1 cut(s) 271
BstBI TTCGAA 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 1 cut(s) 272
BstFNI CGCG 2 cut(s) 335, 359
BstHHI GCGC 2 cut(s) 271, 361
BstKTI GATC 3 cut(s) 146, 420, 588
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 3 cut(s) 143, 417, 585
BstMWI GCNNNNNNNGC 2 cut(s) 263, 367
BstNSI RCATGY 1 cut(s) 319
BstUI CGCG 2 cut(s) 335, 359
BstV1I GCAGC 2 cut(s) 253, 541
BstV2I GAAGAC 1 cut(s) 344
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuRI GGCC 4 cut(s) 203, 242, 282, 476
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 2 cut(s) 271, 361
Cfr13I GGNCC 1 cut(s) 474
CseI GACGC 2 cut(s) 341, 430
CviAII CATG 2 cut(s) 99, 316
DdeI CTNAG 1 cut(s) 272
DpnI GATC 3 cut(s) 145, 419, 587
DpnII GATC 3 cut(s) 143, 417, 585
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 332
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 2 cut(s) 102, 319
FaiI YATR 8 cut(s) 100, 222, 233, 317, 410, 422, 459, 510
FaqI GGGAC 1 cut(s) 513
FatI CATG 2 cut(s) 98, 315
FbaI TGATCA 1 cut(s) 417
Fnu4HI GCNGC 3 cut(s) 267, 477, 530
Fsp4HI GCNGC 3 cut(s) 267, 477, 530
FspBI CTAG 1 cut(s) 171
GlaI GCGC 2 cut(s) 270, 360
GluI GCNGC 3 cut(s) 267, 477, 530
HaeIII GGCC 4 cut(s) 203, 242, 282, 476
HapII CCGG 4 cut(s) 53, 67, 92, 425
HgaI GACGC 2 cut(s) 341, 430
HhaI GCGC 2 cut(s) 271, 361
Hin1II CATG 2 cut(s) 102, 319
Hin6I GCGC 2 cut(s) 269, 359
HinP1I GCGC 2 cut(s) 269, 359
HinfI GANTC 5 cut(s) 27, 83, 227, 373, 448
HpaII CCGG 4 cut(s) 53, 67, 92, 425
Hpy188I TCNGA 3 cut(s) 191, 275, 351
Hpy188III TCNNGA 3 cut(s) 67, 445, 542
Hpy99I CGWCG 1 cut(s) 600
HpyAV CCTTC 2 cut(s) 320, 458
HpyCH4IV ACGT 2 cut(s) 49, 453
HpyCH4V TGCA 2 cut(s) 76, 565
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 367
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 2 cut(s) 49, 453
Hsp92II CATG 2 cut(s) 102, 319
HspAI GCGC 2 cut(s) 269, 359
Kpn2I TCCGGA 1 cut(s) 66
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 3 cut(s) 143, 417, 585
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 8 cut(s) 66, 80, 105, 106, 248, 438, 457, 458
Lsp1109I GCAGC 2 cut(s) 253, 541
LweI GCATC 3 cut(s) 258, 368, 562
MaeI CTAG 1 cut(s) 171
MaeII ACGT 2 cut(s) 49, 453
MaeIII GTNAC 1 cut(s) 404
MalI GATC 3 cut(s) 145, 419, 587
MboI GATC 3 cut(s) 143, 417, 585
MboII GAAGA 4 cut(s) 138, 288, 349, 581
MflI RGATCY 1 cut(s) 143
MluCI AATT 4 cut(s) 87, 114, 494, 522
MlyI GAGTC 2 cut(s) 92, 442
MmeI TCCRAC 3 cut(s) 142, 276, 287
MnlI CCTC 7 cut(s) 173, 272, 357, 360, 379, 549, 571
MroI TCCGGA 1 cut(s) 66
MseI TTAA 1 cut(s) 432
MslI CAYNNNNRTG 1 cut(s) 507
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 4 cut(s) 53, 67, 92, 425
MvnI CGCG 2 cut(s) 335, 359
MwoI GCNNNNNNNGC 2 cut(s) 263, 367
NdeII GATC 3 cut(s) 143, 417, 585
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 2 cut(s) 102, 319
NmeAIII GCCGAG 2 cut(s) 229, 601
NmuCI GTSAC 1 cut(s) 404
NspI RCATGY 1 cut(s) 319
NspV TTCGAA 1 cut(s) 30
PceI AGGCCT 1 cut(s) 282
PciI ACATGT 1 cut(s) 315
PfeI GAWTC 3 cut(s) 27, 227, 373
PkrI GCNGC 3 cut(s) 268, 478, 531
PleI GAGTC 2 cut(s) 91, 442
PpsI GAGTC 2 cut(s) 91, 442
PscI ACATGT 1 cut(s) 315
PspPI GGNCC 1 cut(s) 474
PsuI RGATCY 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 507
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 3 cut(s) 267, 477, 530
Sau3AI GATC 3 cut(s) 143, 417, 585
Sau96I GGNCC 1 cut(s) 474
SchI GAGTC 2 cut(s) 92, 442
SetI ASST 3 cut(s) 10, 52, 456
SfaNI GCATC 3 cut(s) 258, 368, 562
SfuI TTCGAA 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 507
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 4 cut(s) 87, 114, 494, 522
SseBI AGGCCT 1 cut(s) 282
SsiI CCGC 2 cut(s) 257, 477
SspMI CTAG 1 cut(s) 171
StuI AGGCCT 1 cut(s) 282
TaiI ACGT 2 cut(s) 52, 456
TaqI TCGA 3 cut(s) 30, 324, 376
TasI AATT 4 cut(s) 87, 114, 494, 522
TauI GCSGC 1 cut(s) 479
TfiI GAWTC 3 cut(s) 27, 227, 373
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 2 cut(s) 266, 529
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 1 cut(s) 561
XapI RAATTY 1 cut(s) 522
XceI RCATGY 1 cut(s) 319
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.