AT1G27870

Contains the following InterPro domains Polynucleotidyl transferase, ribonuclease H fold (InterPro IPR012337)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000204
Physical Location & Seq
Reverse (-)
9706657 .. 9707298
642 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27870.1

Sequence Viewer

Length: 642 bp
ATGAACGTTTTATATTTTCAAAGAAGACATATTCCCTGGGAAATTACTTTGCGTCTTGCTCAAACTGATGCTCATGAGTATACCGAGGCGAATACAGTAGCACAAATAAATACACGTAGCAGAGGGGTGAGAGAGGAGGATAACCATGACAGATGGAGACGACCTGAAAGAGGATGGATCAAATGCAACTTTGATGGCTCATTCGTTAATGGTGATGTAAAAAGTAAAGCAGGTTGGGTTGTTCGGGATAGTAATGGAAGTTATCTACTGGCGGGGCAGGCTATAGGAAGAAAGGTGGACAATGCTCTGGAGAGTGAAATTCAAGCGCTAATCATTTCAATGCAGCACTGTTGGAGTCATGGTTACAAACGGGTTTGCTTTGAAGGAGATAACAAGATGCTGTTTGACCTAATTAACGGATCCAAGGTATATTTTGGCGTTCACAACTGGATACGGGACATTCATTGGTGGTTAAGGAAGTTTGAATCAATCCATTTCAATTGGATAAGAAGACATCACAATACTCCAGCTGATATTCTTGCTAAAGCTCCTCTCCAAAACCAATCTTTATTCCTGAATCATTTCTGGGTTCCTAATGTAATAACCTATGCTCTACATTGTGACTACACTGCTGATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

213

Amino Acids

25.12

Weight (kDa)

8.68

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 63 - 182 2e-35 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 640
Acc36I ACCTGC 1 cut(s) 221
AccI GTMKAC 1 cut(s) 80
AciI CCGC 1 cut(s) 272
AclI AACGTT 1 cut(s) 6
AclWI GGATC 3 cut(s) 185, 414, 427
AcsI RAATTY 1 cut(s) 318
AfeI AGCGCT 1 cut(s) 327
AfiI CCNNNNNNNGG 1 cut(s) 170
AflIII ACRYGT 1 cut(s) 113
AgsI TTSAA 6 cut(s) 20, 323, 339, 383, 485, 499
AjnI CCWGG 1 cut(s) 35
AluBI AGCT 2 cut(s) 530, 548
AluI AGCT 2 cut(s) 530, 548
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 3 cut(s) 185, 414, 427
Aor51HI AGCGCT 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 343
ApoI RAATTY 1 cut(s) 318
Asp700I GAANNNNTTC 1 cut(s) 581
AspLEI GCGC 1 cut(s) 328
AsuHPI GGTGA 2 cut(s) 139, 224
BamHI GGATCC 1 cut(s) 419
BbsI GAAGAC 2 cut(s) 31, 517
BbvI GCAGC 1 cut(s) 355
BccI CCATC 3 cut(s) 147, 168, 188
BciT130I CCWGG 1 cut(s) 37
BciVI GTATCC 1 cut(s) 444
BcoDI GTCTC 1 cut(s) 151
BfmI CTRYAG 1 cut(s) 282
BfoI RGCGCY 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 221
BfuI GTATCC 1 cut(s) 444
BisI GCNGC 1 cut(s) 344
BlsI GCNGC 1 cut(s) 345
Bme1390I CCNGG 1 cut(s) 37
BmiI GGNNCC 2 cut(s) 421, 591
BmrFI CCNGG 1 cut(s) 37
BmsI GCATC 2 cut(s) 58, 387
BpiI GAAGAC 2 cut(s) 31, 517
BpmI CTGGAG 2 cut(s) 329, 510
BsaAI YACGTR 1 cut(s) 116
BsaJI CCNNGG 4 cut(s) 35, 36, 84, 423
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 273, 452
BseBI CCWGG 1 cut(s) 37
BseDI CCNNGG 4 cut(s) 35, 36, 84, 423
BseGI GGATG 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 170
BseNI ACTGG 2 cut(s) 273, 452
BseRI GAGGAG 2 cut(s) 149, 540
BseXI GCAGC 1 cut(s) 355
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 151
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 470
Bsp143I GATC 2 cut(s) 177, 419
BspACI CCGC 1 cut(s) 272
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 421, 591
BspMI ACCTGC 1 cut(s) 221
BspPI GGATC 3 cut(s) 185, 414, 427
BsrI ACTGG 2 cut(s) 273, 452
BssECI CCNNGG 4 cut(s) 35, 36, 84, 423
BssMI GATC 2 cut(s) 177, 419
BssNAI GTATAC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 423
Bst1107I GTATAC 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 37
Bst4CI ACNGT 2 cut(s) 97, 350
BstBAI YACGTR 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 279
BstENI CCTNNNNNAGG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 179
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 2 cut(s) 180, 422
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 2 cut(s) 177, 419
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNI CCWGG 1 cut(s) 37
BstSCI CCNGG 1 cut(s) 35
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 355
BstV2I GAAGAC 2 cut(s) 31, 517
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BstZ17I GTATAC 1 cut(s) 81
BsuI GTATCC 1 cut(s) 444
BtsCI GGATG 1 cut(s) 179
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 2 cut(s) 346, 627
BveI ACCTGC 1 cut(s) 221
Cac8I GCNNGC 1 cut(s) 279
CciI TCATGA 1 cut(s) 73
CfoI GCGC 1 cut(s) 328
CseI GACGC 1 cut(s) 41
CviAII CATG 3 cut(s) 74, 146, 359
CviJI RGCY 4 cut(s) 198, 281, 530, 548
CviKI_1 RGCY 4 cut(s) 198, 281, 530, 548
DpnI GATC 2 cut(s) 179, 421
DpnII GATC 2 cut(s) 177, 419
Eco130I CCWWGG 1 cut(s) 423
Eco47III AGCGCT 1 cut(s) 327
EcoNI CCTNNNNNAGG 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 35
EcoT14I CCWWGG 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 423
Esp3I CGTCTC 1 cut(s) 151
FaeI CATG 3 cut(s) 77, 149, 362
FaqI GGGAC 1 cut(s) 470
FatI CATG 3 cut(s) 73, 145, 358
FauI CCCGC 1 cut(s) 265
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 1 cut(s) 344
FokI GGATG 1 cut(s) 186
Fsp4HI GCNGC 1 cut(s) 344
GlaI GCGC 1 cut(s) 327
GluI GCNGC 1 cut(s) 344
GsuI CTGGAG 2 cut(s) 329, 510
HaeII RGCGCY 1 cut(s) 329
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 328
Hin1II CATG 3 cut(s) 77, 149, 362
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HinfI GANTC 3 cut(s) 355, 485, 577
HphI GGTGA 2 cut(s) 139, 224
Hpy166II GTNNAC 3 cut(s) 81, 298, 442
Hpy188III TCNNGA 4 cut(s) 74, 245, 308, 574
Hpy8I GTNNAC 3 cut(s) 81, 298, 442
HpyAV CCTTC 1 cut(s) 377
HpyCH4III ACNGT 2 cut(s) 97, 350
HpyCH4IV ACGT 2 cut(s) 6, 115
HpyCH4V TGCA 2 cut(s) 186, 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpySE526I ACGT 2 cut(s) 6, 115
Hsp92II CATG 3 cut(s) 77, 149, 362
HspAI GCGC 1 cut(s) 326
Kzo9I GATC 2 cut(s) 177, 419
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 2 cut(s) 58, 387
MaeII ACGT 2 cut(s) 6, 115
MaeIII GTNAC 2 cut(s) 362, 620
MalI GATC 2 cut(s) 179, 421
MboI GATC 2 cut(s) 177, 419
MboII GAAGA 3 cut(s) 36, 300, 522
MfeI CAATTG 1 cut(s) 499
MflI RGATCY 1 cut(s) 419
MluCI AATT 4 cut(s) 42, 318, 411, 499
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 332
MnlI CCTC 6 cut(s) 79, 116, 127, 130, 164, 561
MroXI GAANNNNTTC 1 cut(s) 581
MseI TTAA 3 cut(s) 207, 414, 473
MslI CAYNNNNRTG 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 530
MspR9I CCNGG 1 cut(s) 37
MunI CAATTG 1 cut(s) 499
MvaI CCWGG 1 cut(s) 37
MwoI GCNNNNNNNGC 1 cut(s) 278
NdeII GATC 2 cut(s) 177, 419
NlaIII CATG 3 cut(s) 77, 149, 362
NlaIV GGNNCC 2 cut(s) 421, 591
NmuCI GTSAC 1 cut(s) 620
PagI TCATGA 1 cut(s) 73
PasI CCCWGGG 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 2 cut(s) 485, 577
PkrI GCNGC 1 cut(s) 345
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
Ppu21I YACGTR 1 cut(s) 116
PsiI TTATAA 1 cut(s) 640
Psp1406I AACGTT 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 35
PspGI CCWGG 1 cut(s) 35
PspN4I GGNNCC 2 cut(s) 421, 591
PsuI RGATCY 1 cut(s) 419
PvuII CAGCTG 1 cut(s) 530
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 3 cut(s) 207, 414, 473
SatI GCNGC 1 cut(s) 344
Sau3AI GATC 2 cut(s) 177, 419
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 1 cut(s) 37
SfaNI GCATC 2 cut(s) 58, 387
SfcI CTRYAG 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 4 cut(s) 42, 318, 411, 499
SsiI CCGC 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 35
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 2 cut(s) 97, 350
TaiI ACGT 2 cut(s) 9, 118
TasI AATT 4 cut(s) 42, 318, 411, 499
TfiI GAWTC 2 cut(s) 485, 577
Tru1I TTAA 3 cut(s) 207, 414, 473
Tru9I TTAA 3 cut(s) 207, 414, 473
TscAI CASTG 2 cut(s) 353, 634
TseFI GTSAC 1 cut(s) 620
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 2 cut(s) 17, 452
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 2 cut(s) 353, 634
XagI CCTNNNNNAGG 1 cut(s) 168
XapI RAATTY 1 cut(s) 318
XmiI GTMKAC 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.