hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 683 |
| AccIII | TCCGGA | 1 cut(s) | 41 |
| AciI | CCGC | 3 cut(s) | 515, 663, 981 |
| AclWI | GGATC | 3 cut(s) | 191, 196, 770 |
| AcsI | RAATTY | 2 cut(s) | 25, 704 |
| AcvI | CACGTG | 1 cut(s) | 821 |
| AfaI | GTAC | 2 cut(s) | 255, 903 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 763, 796 |
| AflIII | ACRYGT | 2 cut(s) | 818, 820 |
| AgsI | TTSAA | 3 cut(s) | 282, 552, 704 |
| AjnI | CCWGG | 1 cut(s) | 333 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 400, 432 |
| AluBI | AGCT | 7 cut(s) | 301, 380, 463, 485, 633, 678, 1027 |
| AluI | AGCT | 7 cut(s) | 301, 380, 463, 485, 633, 678, 1027 |
| Alw26I | GTCTC | 1 cut(s) | 523 |
| AlwI | GGATC | 3 cut(s) | 191, 196, 770 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 973 |
| Aor13HI | TCCGGA | 1 cut(s) | 41 |
| AoxI | GGCC | 1 cut(s) | 331 |
| ApeKI | GCWGC | 7 cut(s) | 11, 151, 463, 675, 983, 1024, 1123 |
| ApoI | RAATTY | 2 cut(s) | 25, 704 |
| AseI | ATTAAT | 1 cut(s) | 716 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 885, 1167 |
| AspS9I | GGNCC | 1 cut(s) | 125 |
| AsuC2I | CCSGG | 1 cut(s) | 758 |
| AsuHPI | GGTGA | 5 cut(s) | 515, 778, 794, 959, 970 |
| AsuII | TTCGAA | 1 cut(s) | 400 |
| AvaII | GGWCC | 1 cut(s) | 125 |
| BbrPI | CACGTG | 1 cut(s) | 821 |
| BbsI | GAAGAC | 2 cut(s) | 821, 996 |
| BbvI | GCAGC | 6 cut(s) | 138, 450, 687, 970, 1036, 1110 |
| BccI | CCATC | 4 cut(s) | 24, 323, 1064, 1085 |
| BceAI | ACGGC | 1 cut(s) | 128 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 605, 639 |
| BciT130I | CCWGG | 1 cut(s) | 335 |
| BciVI | GTATCC | 1 cut(s) | 913 |
| BclI | TGATCA | 1 cut(s) | 487 |
| BcnI | CCSGG | 1 cut(s) | 758 |
| BcoDI | GTCTC | 1 cut(s) | 523 |
| BfaI | CTAG | 3 cut(s) | 302, 749, 1061 |
| BfmI | CTRYAG | 3 cut(s) | 423, 987, 1124 |
| BfuAI | ACCTGC | 1 cut(s) | 683 |
| BfuI | GTATCC | 1 cut(s) | 913 |
| BisI | GCNGC | 7 cut(s) | 12, 152, 464, 676, 984, 1025, 1124 |
| BlsI | GCNGC | 7 cut(s) | 13, 153, 465, 677, 985, 1026, 1125 |
| BmcAI | AGTACT | 1 cut(s) | 903 |
| Bme1390I | CCNGG | 2 cut(s) | 335, 758 |
| Bme18I | GGWCC | 1 cut(s) | 125 |
| BmgT120I | GGNCC | 1 cut(s) | 125 |
| BmrFI | CCNGG | 2 cut(s) | 335, 758 |
| BmsI | GCATC | 4 cut(s) | 70, 163, 246, 855 |
| BpiI | GAAGAC | 2 cut(s) | 821, 996 |
| BpmI | CTGGAG | 2 cut(s) | 161, 177 |
| Bpu14I | TTCGAA | 1 cut(s) | 400 |
| BpuMI | CCSGG | 1 cut(s) | 758 |
| BsaAI | YACGTR | 1 cut(s) | 821 |
| BsaJI | CCNNGG | 2 cut(s) | 265, 687 |
| BsaWI | WCCGGW | 1 cut(s) | 41 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 763, 796 |
| Bse1I | ACTGG | 2 cut(s) | 845, 909 |
| Bse3DI | GCAATG | 1 cut(s) | 40 |
| BseAI | TCCGGA | 1 cut(s) | 41 |
| BseBI | CCWGG | 1 cut(s) | 335 |
| BseDI | CCNNGG | 2 cut(s) | 265, 687 |
| BseGI | GGATG | 1 cut(s) | 766 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 763, 796 |
| BseMI | GCAATG | 1 cut(s) | 40 |
| BseMII | CTCAG | 1 cut(s) | 945 |
| BseNI | ACTGG | 2 cut(s) | 845, 909 |
| BseRI | GAGGAG | 2 cut(s) | 443, 882 |
| BseXI | GCAGC | 6 cut(s) | 138, 450, 687, 970, 1036, 1110 |
| BseYI | CCCAGC | 2 cut(s) | 633, 1027 |
| BshFI | GGCC | 1 cut(s) | 333 |
| BsiSI | CCGG | 2 cut(s) | 42, 757 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 763, 796 |
| BsmAI | GTCTC | 1 cut(s) | 523 |
| BsnI | GGCC | 1 cut(s) | 333 |
| Bsp119I | TTCGAA | 1 cut(s) | 400 |
| Bsp13I | TCCGGA | 1 cut(s) | 41 |
| Bsp1407I | TGTACA | 1 cut(s) | 253 |
| Bsp143I | GATC | 6 cut(s) | 64, 106, 188, 196, 487, 775 |
| Bsp19I | CCATGG | 1 cut(s) | 687 |
| BspACI | CCGC | 3 cut(s) | 515, 663, 981 |
| BspANI | GGCC | 1 cut(s) | 333 |
| BspCNI | CTCAG | 1 cut(s) | 946 |
| BspEI | TCCGGA | 1 cut(s) | 41 |
| BspMAI | CTGCAG | 1 cut(s) | 1128 |
| BspMI | ACCTGC | 1 cut(s) | 683 |
| BspPI | GGATC | 3 cut(s) | 191, 196, 770 |
| BspT104I | TTCGAA | 1 cut(s) | 400 |
| BsrDI | GCAATG | 1 cut(s) | 40 |
| BsrGI | TGTACA | 1 cut(s) | 253 |
| BsrI | ACTGG | 2 cut(s) | 845, 909 |
| BssECI | CCNNGG | 2 cut(s) | 265, 687 |
| BssMI | GATC | 6 cut(s) | 64, 106, 188, 196, 487, 775 |
| BssT1I | CCWWGG | 2 cut(s) | 265, 687 |
| Bst2UI | CCWGG | 1 cut(s) | 335 |
| Bst4CI | ACNGT | 1 cut(s) | 361 |
| BstAUI | TGTACA | 1 cut(s) | 253 |
| BstBAI | YACGTR | 1 cut(s) | 821 |
| BstBI | TTCGAA | 1 cut(s) | 400 |
| BstC8I | GCNNGC | 4 cut(s) | 382, 386, 618, 1045 |
| BstDEI | CTNAG | 2 cut(s) | 954, 1118 |
| BstDSI | CCRYGG | 1 cut(s) | 687 |
| BstEII | GGTNACC | 2 cut(s) | 800, 976 |
| BstF5I | GGATG | 1 cut(s) | 766 |
| BstKTI | GATC | 6 cut(s) | 67, 109, 191, 199, 490, 778 |
| BstMAI | GTCTC | 1 cut(s) | 523 |
| BstMBI | GATC | 6 cut(s) | 64, 106, 188, 196, 487, 775 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 989 |
| BstNI | CCWGG | 1 cut(s) | 335 |
| BstNSI | RCATGY | 1 cut(s) | 388 |
| BstPI | GGTNACC | 2 cut(s) | 800, 976 |
| BstSCI | CCNGG | 2 cut(s) | 333, 756 |
| BstSFI | CTRYAG | 3 cut(s) | 423, 987, 1124 |
| BstV1I | GCAGC | 6 cut(s) | 138, 450, 687, 970, 1036, 1110 |
| BstV2I | GAAGAC | 2 cut(s) | 821, 996 |
| BstX2I | RGATCY | 2 cut(s) | 188, 196 |
| BstYI | RGATCY | 2 cut(s) | 188, 196 |
| BsuI | GTATCC | 1 cut(s) | 913 |
| BsuRI | GGCC | 1 cut(s) | 333 |
| BtgI | CCRYGG | 1 cut(s) | 687 |
| BtgZI | GCGATG | 1 cut(s) | 1096 |
| BtsCI | GGATG | 1 cut(s) | 766 |
| BtsIMutI | CAGTG | 3 cut(s) | 300, 816, 852 |
| BveI | ACCTGC | 1 cut(s) | 683 |
| Cac8I | GCNNGC | 4 cut(s) | 382, 386, 618, 1045 |
| CaiI | CAGNNNCTG | 1 cut(s) | 973 |
| Cfr13I | GGNCC | 1 cut(s) | 125 |
| Csp6I | GTAC | 2 cut(s) | 254, 902 |
| CviAII | CATG | 2 cut(s) | 385, 688 |
| CviQI | GTAC | 2 cut(s) | 254, 902 |
| DdeI | CTNAG | 2 cut(s) | 954, 1118 |
| DpnI | GATC | 6 cut(s) | 66, 108, 190, 198, 489, 777 |
| DpnII | GATC | 6 cut(s) | 64, 106, 188, 196, 487, 775 |
| DraI | TTTAAA | 2 cut(s) | 216, 477 |
| Eco130I | CCWWGG | 2 cut(s) | 265, 687 |
| Eco47I | GGWCC | 1 cut(s) | 125 |
| Eco72I | CACGTG | 1 cut(s) | 821 |
| Eco91I | GGTNACC | 2 cut(s) | 800, 976 |
| EcoO65I | GGTNACC | 2 cut(s) | 800, 976 |
| EcoRII | CCWGG | 1 cut(s) | 333 |
| EcoT14I | CCWWGG | 2 cut(s) | 265, 687 |
| ErhI | CCWWGG | 2 cut(s) | 265, 687 |
| FaeI | CATG | 2 cut(s) | 388, 691 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 544, 576 |
| FatI | CATG | 2 cut(s) | 384, 687 |
| FauI | CCCGC | 1 cut(s) | 522 |
| FbaI | TGATCA | 1 cut(s) | 487 |
| Fnu4HI | GCNGC | 7 cut(s) | 12, 152, 464, 676, 984, 1025, 1124 |
| FokI | GGATG | 1 cut(s) | 773 |
| Fsp4HI | GCNGC | 7 cut(s) | 12, 152, 464, 676, 984, 1025, 1124 |
| FspBI | CTAG | 3 cut(s) | 302, 749, 1061 |
| GluI | GCNGC | 7 cut(s) | 12, 152, 464, 676, 984, 1025, 1124 |
| GsaI | CCCAGC | 2 cut(s) | 637, 1031 |
| GsuI | CTGGAG | 2 cut(s) | 161, 177 |
| HaeIII | GGCC | 1 cut(s) | 333 |
| HapII | CCGG | 2 cut(s) | 42, 757 |
| Hin1II | CATG | 2 cut(s) | 388, 691 |
| HincII | GTYRAC | 1 cut(s) | 166 |
| HindII | GTYRAC | 1 cut(s) | 166 |
| HindIII | AAGCTT | 1 cut(s) | 378 |
| HinfI | GANTC | 7 cut(s) | 416, 493, 647, 841, 874, 886, 1016 |
| HpaII | CCGG | 2 cut(s) | 42, 757 |
| HphI | GGTGA | 5 cut(s) | 515, 778, 794, 959, 970 |
| Hpy166II | GTNNAC | 5 cut(s) | 128, 166, 254, 364, 727 |
| Hpy188I | TCNGA | 5 cut(s) | 79, 492, 498, 652, 737 |
| Hpy188III | TCNNGA | 4 cut(s) | 20, 42, 194, 878 |
| Hpy8I | GTNNAC | 5 cut(s) | 128, 166, 254, 364, 727 |
| Hpy99I | CGWCG | 1 cut(s) | 114 |
| HpyAV | CCTTC | 2 cut(s) | 528, 664 |
| HpyCH4III | ACNGT | 1 cut(s) | 361 |
| HpyCH4IV | ACGT | 2 cut(s) | 729, 820 |
| HpyCH4V | TGCA | 9 cut(s) | 83, 154, 237, 274, 384, 466, 794, 1024, 1126 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 989 |
| HpyF3I | CTNAG | 2 cut(s) | 954, 1118 |
| HpySE526I | ACGT | 2 cut(s) | 729, 820 |
| Hsp92II | CATG | 2 cut(s) | 388, 691 |
| Kpn2I | TCCGGA | 1 cut(s) | 41 |
| Ksp22I | TGATCA | 1 cut(s) | 487 |
| Kzo9I | GATC | 6 cut(s) | 64, 106, 188, 196, 487, 775 |
| LmnI | GCTCC | 1 cut(s) | 430 |
| Lsp1109I | GCAGC | 6 cut(s) | 138, 450, 687, 970, 1036, 1110 |
| LweI | GCATC | 4 cut(s) | 70, 163, 246, 855 |
| MaeI | CTAG | 3 cut(s) | 302, 749, 1061 |
| MaeII | ACGT | 2 cut(s) | 729, 820 |
| MaeIII | GTNAC | 4 cut(s) | 626, 766, 800, 976 |
| MalI | GATC | 6 cut(s) | 66, 108, 190, 198, 489, 777 |
| MboI | GATC | 6 cut(s) | 64, 106, 188, 196, 487, 775 |
| MboII | GAAGA | 7 cut(s) | 16, 411, 528, 540, 651, 826, 996 |
| MfeI | CAATTG | 1 cut(s) | 88 |
| MflI | RGATCY | 2 cut(s) | 188, 196 |
| MluCI | AATT | 6 cut(s) | 25, 88, 116, 544, 704, 717 |
| MlyI | GAGTC | 2 cut(s) | 641, 1025 |
| MmeI | TCCRAC | 2 cut(s) | 691, 1140 |
| MnlI | CCTC | 8 cut(s) | 421, 731, 900, 903, 943, 981, 1133, 1182 |
| MroI | TCCGGA | 1 cut(s) | 41 |
| MroXI | GAANNNNTTC | 2 cut(s) | 885, 1167 |
| MseI | TTAA | 4 cut(s) | 215, 476, 716, 720 |
| MspA1I | CMGCKG | 4 cut(s) | 485, 633, 983, 1027 |
| MspI | CCGG | 2 cut(s) | 42, 757 |
| MspR9I | CCNGG | 2 cut(s) | 335, 758 |
| MunI | CAATTG | 1 cut(s) | 88 |
| MvaI | CCWGG | 1 cut(s) | 335 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 989 |
| NciI | CCSGG | 1 cut(s) | 758 |
| NcoI | CCATGG | 1 cut(s) | 687 |
| NdeII | GATC | 6 cut(s) | 64, 106, 188, 196, 487, 775 |
| NlaIII | CATG | 2 cut(s) | 388, 691 |
| NmuCI | GTSAC | 3 cut(s) | 766, 800, 976 |
| NspI | RCATGY | 1 cut(s) | 388 |
| NspV | TTCGAA | 1 cut(s) | 400 |
| PacI | TTAATTAA | 1 cut(s) | 720 |
| PaeI | GCATGC | 1 cut(s) | 388 |
| PdmI | GAANNNNTTC | 2 cut(s) | 885, 1167 |
| PfeI | GAWTC | 5 cut(s) | 416, 493, 841, 874, 886 |
| PfoI | TCCNGGA | 1 cut(s) | 756 |
| PkrI | GCNGC | 7 cut(s) | 13, 153, 465, 677, 985, 1026, 1125 |
| PleI | GAGTC | 2 cut(s) | 641, 1024 |
| PmaCI | CACGTG | 1 cut(s) | 821 |
| PmlI | CACGTG | 1 cut(s) | 821 |
| PpsI | GAGTC | 2 cut(s) | 641, 1024 |
| Ppu21I | YACGTR | 1 cut(s) | 821 |
| PshBI | ATTAAT | 1 cut(s) | 716 |
| Psp6I | CCWGG | 1 cut(s) | 333 |
| PspCI | CACGTG | 1 cut(s) | 821 |
| PspEI | GGTNACC | 2 cut(s) | 800, 976 |
| PspFI | CCCAGC | 2 cut(s) | 633, 1027 |
| PspGI | CCWGG | 1 cut(s) | 333 |
| PspPI | GGNCC | 1 cut(s) | 125 |
| PstI | CTGCAG | 1 cut(s) | 1128 |
| PstNI | CAGNNNCTG | 1 cut(s) | 973 |
| PsuI | RGATCY | 2 cut(s) | 188, 196 |
| PvuII | CAGCTG | 3 cut(s) | 485, 633, 1027 |
| RsaI | GTAC | 2 cut(s) | 255, 903 |
| RsaNI | GTAC | 2 cut(s) | 254, 902 |
| SaqAI | TTAA | 4 cut(s) | 215, 476, 716, 720 |
| SatI | GCNGC | 7 cut(s) | 12, 152, 464, 676, 984, 1025, 1124 |
| Sau3AI | GATC | 6 cut(s) | 64, 106, 188, 196, 487, 775 |
| Sau96I | GGNCC | 1 cut(s) | 125 |
| ScaI | AGTACT | 1 cut(s) | 903 |
| SchI | GAGTC | 2 cut(s) | 641, 1025 |
| ScrFI | CCNGG | 2 cut(s) | 335, 758 |
| SfaNI | GCATC | 4 cut(s) | 70, 163, 246, 855 |
| SfcI | CTRYAG | 3 cut(s) | 423, 987, 1124 |
| SfuI | TTCGAA | 1 cut(s) | 400 |
| SinI | GGWCC | 1 cut(s) | 125 |
| SphI | GCATGC | 1 cut(s) | 388 |
| Sse9I | AATT | 6 cut(s) | 25, 88, 116, 544, 704, 717 |
| SsiI | CCGC | 3 cut(s) | 515, 663, 981 |
| SspI | AATATT | 1 cut(s) | 355 |
| SspMI | CTAG | 3 cut(s) | 302, 749, 1061 |
| StyD4I | CCNGG | 2 cut(s) | 333, 756 |
| StyI | CCWWGG | 2 cut(s) | 265, 687 |
| TaaI | ACNGT | 1 cut(s) | 361 |
| TaiI | ACGT | 2 cut(s) | 732, 823 |
| TaqI | TCGA | 3 cut(s) | 109, 400, 872 |
| TasI | AATT | 6 cut(s) | 25, 88, 116, 544, 704, 717 |
| TatI | WGTACW | 2 cut(s) | 253, 901 |
| TfiI | GAWTC | 5 cut(s) | 416, 493, 841, 874, 886 |
| Tru1I | TTAA | 4 cut(s) | 215, 476, 716, 720 |
| Tru9I | TTAA | 4 cut(s) | 215, 476, 716, 720 |
| TscAI | CASTG | 3 cut(s) | 300, 816, 852 |
| TseFI | GTSAC | 3 cut(s) | 766, 800, 976 |
| TseI | GCWGC | 7 cut(s) | 11, 151, 463, 675, 983, 1024, 1123 |
| Tsp45I | GTSAC | 3 cut(s) | 766, 800, 976 |
| TspDTI | ATGAA | 5 cut(s) | 17, 321, 333, 390, 870 |
| TspRI | CASTG | 3 cut(s) | 300, 816, 852 |
| VpaK11BI | GGWCC | 1 cut(s) | 125 |
| VspI | ATTAAT | 1 cut(s) | 716 |
| XapI | RAATTY | 2 cut(s) | 25, 704 |
| XceI | RCATGY | 1 cut(s) | 388 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 201, 694 |
| XmnI | GAANNNNTTC | 2 cut(s) | 885, 1167 |
| XspI | CTAG | 3 cut(s) | 302, 749, 1061 |
| ZrmI | AGTACT | 1 cut(s) | 903 |