pycom03g12410

ribonuclease H protein At1g65750

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000204
Physical Location & Seq
Forward (+)
12848177 .. 12849508
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g12410.2

Sequence Viewer

Length: 645 bp
ATGCCTGCCAACAAAGAAAAATCATATGCGGATGGAAATGGCCGGAAGAAACCAAGCTTTTGGGTGCTCAAGGTTAATTGTGATGCAGCGTGGGATTCAAAAACTAAGAAAGGAGGAGTTGGTTGGGTCGTACGAATTTTTGCGGGCATGTGGCATTTCTCAGGTGGCACGGGAGGCCTGTTTTTCCCTTCTGCAGTGGCGGGGGAGGTTGCTGCGGTGAGAGCAACTTCATACGCCTGTTTGAAGGAAGGGATCACAGGAATTATTGTGGTATCTGATGCTAAGATTGTCCTAAACATGATCACAAGAGAATTACAATTGGGAGCTGCGGATGTTGGTGTCTTGGAGTTCGCATACTCTCCTCATCGTGCCAACAACACTGCGCATGTAGTTGCAGCAGATGTCTTCAAGACAGAAGAAAATGTGATGTGGGATTGTATCGGTCCTGATTTTCTTTTCAATATTTTGGCAAAGGATATTGAACGGAAGACGTATGGTCCCCACGTTTCTCTTTACTGCAATGTGAAATTGAGGGTAGGCCTTAGCCTAAAGAAGAAGAAGCTGGTGCCCAGGTCCAAGACTTGGGCTAGGTATTATGCAGGGTGGTTTAGAGGCCCACAACTAGCTGGGTTGCAGACGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

215

Amino Acids

23.44

Weight (kDa)

9.63

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04625 AT1G27220 AT1G27250 AT1G27870 AT1G47497 AT1G47497 AT1G50160 AT1G52990 AT1G52990 AT1G77815 AT2G02650 AT2G04420 AT2G11205 AT2G13980 AT2G33160 AT2G46460 AT3G09510 AT3G23320 AT3G25270 AT3G32130 AT4G03292 AT4G09775 AT5G26617 AT5G44470 AT5G52115 AT5G65005
fragaria_vesca FvH4_1g25222 FvH4_1g28481 FvH4_3g03751 FvH4_3g24081 FvH4_3g35341 FvH4_5g31961 FvH4_6g20891 FvH4_6g23253 FvH4_6g31361 FvH4_6g53051
malus_domestica MD14G1209300.v1.1
prunus_persica Prupe.1G168800_v2.0.a1 Prupe.1G509200_v2.0.a1 Prupe.2G042000_v2.0.a1 Prupe.2G133200_v2.0.a1 Prupe.2G140300_v2.0.a1 Prupe.3G103800_v2.0.a1 Prupe.4G217300_v2.0.a1 Prupe.4G245300_v2.0.a1 Prupe.5G002600_v2.0.a1 Prupe.5G134600_v2.0.a1 Prupe.6G185900_v2.0.a1 Prupe.6G308300_v2.0.a1 Prupe.7G010700_v2.0.a1 Prupe.8G078100_v2.0.a1 Prupe.8G128400_v2.0.a1
pyrus_communis pycom01g14230 pycom03g12410 pycom08g15160 pycom08g21670 pycom10g25730 pycom10g25740 pycom11g15380 pycom14g10440 pycom14g11510 pycom15g31840 pycom16g02370
rosa_chinensis RchiOBHm_Chr4g0440421 RchiOBHm_Chr5g0005841 RchiOBHm_Chr6g0289481 RchiOBHm_Chr7g0218671
rosa_laevigata RLG00000011653 RLG00000031330 RLG00000031331 RLG00000032722
rosa_multiflora Rmu_co8243061.1_g000001 Rmu_sc0000470.1_g000036 Rmu_sc0000581.1_g000008 Rmu_sc0000666.1_g000017 Rmu_sc0000905.1_g000019 Rmu_sc0000976.1_g000013 Rmu_sc0001066.1_g000001 Rmu_sc0001103.1_g000007 Rmu_sc0001180.1_g000001 Rmu_sc0001643.1_g000034 Rmu_sc0001759.1_g000007 Rmu_sc0001850.1_g000051 Rmu_sc0001910.1_g000008 Rmu_sc0001981.1_g000015 Rmu_sc0001981.1_g000029 Rmu_sc0002209.1_g000015 Rmu_sc0002406.1_g000010 Rmu_sc0003743.1_g000002 Rmu_sc0006416.1_g000026 Rmu_sc0006513.1_g000006 Rmu_sc0006577.1_g000010 Rmu_sc0014652.1_g000001 Rmu_sc0016202.1_g000003
rosa_roxburghii Rroxscaffold_1G00001530 Rroxscaffold_1G00047250 Rroxscaffold_5G00340720 Rroxscaffold_7G00171110 Rroxscaffold_7G00177650
rosa_rugosa Rorug01G0044800 Rorug01G0204500 Rorug01G0224700 Rorug01G0267800 Rorug02G0038400 Rorug02G0107100 Rorug02G0180700 Rorug02G0363800 Rorug02G0374300 Rorug02G0491400 Rorug02G0491400 Rorug03G0104200 Rorug03G0109800 Rorug03G0334200 Rorug04G0007100 Rorug04G0061400.1 Rorug04G0080000 Rorug05G0152200 Rorug05G0188800 Rorug05G0234800 Rorug05G0239900 Rorug05G0284800 Rorug06G0044700 Rorug06G0205700 Rorug06G0266900 Rorug06G0440700 Rorug07G0037400 Rorug07G0192100 Rorug07G0196900 Rorug07G0237500 Rorug07G0336400.1 RorugPtG0002500.1
rosa_samantha Rh1BG014800 Rh1CG017500 Rh2CG332500 Rh3BG371800 Rh5AG046000 Rh5AG046100 Rh5BG044700 Rh5BG044800 Rh5BG248000 Rh5CG053600 Rh5CG053700 Rh5DG044500 Rh5DG044600 Rh6AG052800 Rh6AG315400 Rh6DG021400 Rh7AG409500
rosa_wichuraiana Rw3G007750 Rw3G018750 Rw4G008820 Rw5G004630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 384
AccB1I GGYRCC 1 cut(s) 565
AccB7I CCANNNNNTGG 1 cut(s) 582
AciI CCGC 5 cut(s) 29, 143, 200, 215, 329
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 1 cut(s) 135
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 582
AgsI TTSAA 5 cut(s) 99, 244, 409, 460, 482
AhdI GACNNNNNGTC 1 cut(s) 495
AjnI CCWGG 1 cut(s) 569
AluBI AGCT 4 cut(s) 57, 326, 562, 626
AluI AGCT 4 cut(s) 57, 326, 562, 626
Alw21I GWGCWC 1 cut(s) 69
AlwI GGATC 1 cut(s) 260
AoxI GGCC 4 cut(s) 40, 175, 538, 613
ApeKI GCWGC 4 cut(s) 86, 212, 326, 395
ApoI RAATTY 1 cut(s) 135
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 4 cut(s) 443, 497, 573, 614
AsuHPI GGTGA 1 cut(s) 229
AvaII GGWCC 3 cut(s) 443, 497, 573
BaeGI GKGCMC 1 cut(s) 570
BanI GGYRCC 1 cut(s) 565
BbsI GAAGAC 2 cut(s) 397, 494
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 4 cut(s) 98, 199, 313, 407
BccI CCATC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 571
BclI TGATCA 1 cut(s) 300
BfaI CTAG 2 cut(s) 588, 623
BfmI CTRYAG 1 cut(s) 192
BisI GCNGC 4 cut(s) 87, 213, 327, 396
BlsI GCNGC 4 cut(s) 88, 214, 328, 397
Bme1390I CCNGG 1 cut(s) 571
Bme18I GGWCC 3 cut(s) 443, 497, 573
BmeRI GACNNNNNGTC 1 cut(s) 495
BmgT120I GGNCC 4 cut(s) 443, 497, 573, 614
BmiI GGNNCC 2 cut(s) 499, 567
BmrFI CCNGG 1 cut(s) 571
BmsI GCATC 2 cut(s) 73, 268
BpiI GAAGAC 2 cut(s) 397, 494
Bpu10I CCTNAGC 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 569
Bsc4I CCNNNNNNNGG 1 cut(s) 582
Bse3DI GCAATG 1 cut(s) 526
BseBI CCWGG 1 cut(s) 571
BseDI CCNNGG 1 cut(s) 569
BseGI GGATG 2 cut(s) 37, 337
BseLI CCNNNNNNNGG 1 cut(s) 582
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 129, 351
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 4 cut(s) 98, 199, 313, 407
BseYI CCCAGC 1 cut(s) 626
BshFI GGCC 4 cut(s) 42, 177, 540, 615
BshNI GGYRCC 1 cut(s) 565
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 43
BsiWI CGTACG 1 cut(s) 130
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 1 cut(s) 582
BsmFI GGGAC 1 cut(s) 483
BsnI GGCC 4 cut(s) 42, 177, 540, 615
Bsp1286I GDGCHC 2 cut(s) 69, 570
Bsp143I GATC 2 cut(s) 252, 300
BspACI CCGC 5 cut(s) 29, 143, 200, 215, 329
BspANI GGCC 4 cut(s) 42, 177, 540, 615
BspCNI CTCAG 1 cut(s) 173
BspLI GGNNCC 2 cut(s) 499, 567
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 260
BspT107I GGYRCC 1 cut(s) 565
BsrDI GCAATG 1 cut(s) 526
BssECI CCNNGG 1 cut(s) 569
BssMI GATC 2 cut(s) 252, 300
Bst2UI CCWGG 1 cut(s) 571
BstC8I GCNNGC 2 cut(s) 6, 145
BstDEI CTNAG 4 cut(s) 105, 160, 282, 542
BstF5I GGATG 2 cut(s) 37, 337
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 2 cut(s) 255, 303
BstMBI GATC 2 cut(s) 252, 300
BstMWI GCNNNNNNNGC 2 cut(s) 174, 221
BstNI CCWGG 1 cut(s) 571
BstNSI RCATGY 2 cut(s) 151, 389
BstSCI CCNGG 1 cut(s) 569
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 570
BstV1I GCAGC 4 cut(s) 98, 199, 313, 407
BstV2I GAAGAC 2 cut(s) 397, 494
BstXI CCANNNNNNTGG 1 cut(s) 60
BsuRI GGCC 4 cut(s) 42, 177, 540, 615
BtsCI GGATG 2 cut(s) 37, 337
BtsI GCAGTG 2 cut(s) 201, 378
BtsIMutI CAGTG 2 cut(s) 201, 378
Cac8I GCNNGC 2 cut(s) 6, 145
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 4 cut(s) 443, 497, 573, 614
Csp6I GTAC 1 cut(s) 131
CviAII CATG 3 cut(s) 148, 298, 386
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 4 cut(s) 105, 160, 282, 542
DpnI GATC 2 cut(s) 254, 302
DpnII GATC 2 cut(s) 252, 300
DriI GACNNNNNGTC 1 cut(s) 495
EaeI YGGCCR 1 cut(s) 40
Eam1105I GACNNNNNGTC 1 cut(s) 495
Eco147I AGGCCT 2 cut(s) 177, 540
Eco47I GGWCC 3 cut(s) 443, 497, 573
EcoRII CCWGG 1 cut(s) 569
FaeI CATG 3 cut(s) 151, 301, 389
FaiI YATR 9 cut(s) 25, 27, 149, 232, 299, 355, 387, 495, 597
FaqI GGGAC 1 cut(s) 483
FatI CATG 3 cut(s) 147, 297, 385
FauI CCCGC 2 cut(s) 136, 193
FauNDI CATATG 1 cut(s) 25
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 4 cut(s) 87, 213, 327, 396
FokI GGATG 2 cut(s) 44, 344
Fsp4HI GCNGC 4 cut(s) 87, 213, 327, 396
FspBI CTAG 2 cut(s) 588, 623
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 1 cut(s) 384
GluI GCNGC 4 cut(s) 87, 213, 327, 396
GsaI CCCAGC 1 cut(s) 630
HaeIII GGCC 4 cut(s) 42, 177, 540, 615
HapII CCGG 1 cut(s) 43
HhaI GCGC 1 cut(s) 385
Hin1II CATG 3 cut(s) 151, 301, 389
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 1 cut(s) 95
HpaII CCGG 1 cut(s) 43
HphI GGTGA 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 2 cut(s) 409, 446
HpyAV CCTTC 3 cut(s) 198, 238, 242
HpyCH4IV ACGT 2 cut(s) 491, 504
HpyCH4V TGCA 6 cut(s) 86, 194, 395, 519, 599, 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 221
HpyF3I CTNAG 4 cut(s) 105, 160, 282, 542
HpySE526I ACGT 2 cut(s) 491, 504
Hsp92II CATG 3 cut(s) 151, 301, 389
HspAI GCGC 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 2 cut(s) 252, 300
LmnI GCTCC 1 cut(s) 323
Lsp1109I GCAGC 4 cut(s) 98, 199, 313, 407
LweI GCATC 2 cut(s) 73, 268
MaeI CTAG 2 cut(s) 588, 623
MaeII ACGT 2 cut(s) 491, 504
MalI GATC 2 cut(s) 254, 302
MboI GATC 2 cut(s) 252, 300
MboII GAAGA 6 cut(s) 58, 397, 428, 499, 565, 568
MfeI CAATTG 1 cut(s) 317
MhlI GDGCHC 2 cut(s) 69, 570
MluCI AATT 6 cut(s) 76, 135, 261, 311, 317, 527
MnlI CCTC 6 cut(s) 107, 167, 199, 372, 525, 605
MseI TTAA 1 cut(s) 75
MspI CCGG 1 cut(s) 43
MspR9I CCNGG 1 cut(s) 571
MunI CAATTG 1 cut(s) 317
MvaI CCWGG 1 cut(s) 571
MwoI GCNNNNNNNGC 2 cut(s) 174, 221
NdeI CATATG 1 cut(s) 25
NdeII GATC 2 cut(s) 252, 300
NlaIII CATG 3 cut(s) 151, 301, 389
NlaIV GGNNCC 2 cut(s) 499, 567
NsbI TGCGCA 1 cut(s) 384
NspI RCATGY 2 cut(s) 151, 389
PceI AGGCCT 2 cut(s) 177, 540
PfeI GAWTC 1 cut(s) 95
Pfl23II CGTACG 1 cut(s) 130
PflMI CCANNNNNTGG 1 cut(s) 582
PkrI GCNGC 4 cut(s) 88, 214, 328, 397
Psp6I CCWGG 1 cut(s) 569
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 1 cut(s) 569
PspLI CGTACG 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 499, 567
PspPI GGNCC 4 cut(s) 443, 497, 573, 614
PstI CTGCAG 1 cut(s) 196
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 4 cut(s) 87, 213, 327, 396
Sau3AI GATC 2 cut(s) 252, 300
Sau96I GGNCC 4 cut(s) 443, 497, 573, 614
ScrFI CCNGG 1 cut(s) 571
SduI GDGCHC 2 cut(s) 69, 570
SfaNI GCATC 2 cut(s) 73, 268
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 3 cut(s) 443, 497, 573
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 6 cut(s) 76, 135, 261, 311, 317, 527
SseBI AGGCCT 2 cut(s) 177, 540
SsiI CCGC 5 cut(s) 29, 143, 200, 215, 329
SspI AATATT 1 cut(s) 463
SspMI CTAG 2 cut(s) 588, 623
StuI AGGCCT 2 cut(s) 177, 540
StyD4I CCNGG 1 cut(s) 569
TaiI ACGT 2 cut(s) 494, 507
TaqII GACCGA 1 cut(s) 431
TasI AATT 6 cut(s) 76, 135, 261, 311, 317, 527
TfiI GAWTC 1 cut(s) 95
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 2 cut(s) 201, 385
TseI GCWGC 4 cut(s) 86, 212, 326, 395
TspDTI ATGAA 1 cut(s) 219
TspGWI ACGGA 1 cut(s) 499
TspRI CASTG 2 cut(s) 201, 385
Van91I CCANNNNNTGG 1 cut(s) 582
VpaK11BI GGWCC 3 cut(s) 443, 497, 573
XapI RAATTY 1 cut(s) 135
XceI RCATGY 2 cut(s) 151, 389
XspI CTAG 2 cut(s) 588, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.