MD16G1009400.v1.1

SWI SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD H box

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017196
Physical Location & Seq
Forward (+)
703728 .. 709721
5994 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1009400.v1.1.491

Sequence Viewer

Length: 2241 bp
ATGAAGCGCGGGTTCGACGATTTCGAAATCTCCGACGAGGACTGGGAGGAGCACTCCTCCGCCTTCAAGCCCTCCCGCGTCCTCAAGAAGCCTCGCACCCCAACGCCGCGCCCAATCGAGTCCTTCGCCTTCCGATCATCCCCCAAGCCCCAACAACTCTACGACGAAGATGAAGACTCCGATTGCGTCGAGATTAAACCCGAACTGGAGGACAACGATTCCGATTGTGTCGTGATTAAGGACGAATTGGAGGACGACGACGCCGACGAGGTCCCGGAGGCAAGGACGGTCAATCGTGGCCGTCGGTTCGTGGTCGAGGACGAGGATAGCGATGGGGATTGGGCTAATATTGACTCAACGAGTGAGGAAGAGGAGGATGCGGTAGACGAGTTGGAGGATGACGATGTGGTTGGGAAAGCTTTGCAGAAGTGTGCCAAAATATCGGCGGATTTGAGAAAGGAGCTCCACGGGTCGTCGGCTCCGGCGGTGTCTGATAGGTACGCCGAAGTGGAAGCTGCCTCTGTGAGGATTGTCAATCAGGATGACATTATTGAGGCATGTAGGTCTGAGGATTCCGATTTTCAACCGATTCTCAAGCCGTACCAGTTGGTTGGTGTTAACTTTCTTCTTCTGCTGTATCAGAAGGGTATTGGCGGAGCCATTTTGGCAGACGAGATGGGTCTCGGGAAGACCATTCAGGCTATAACGTACTTGATGTTATTGAAACATTTAAATGATGACCCGGGTCCACATTTGATTGTATGCCCTGCTTCTGTTTTGGAAAATTGGGAAAGAGAACTTAAAAAGTGGTGCCCGTCATTCACTGTTCTCCAGTATCATGGGGCTGCAAGATCGGCTTATTCAAAAGAGTTGAACTCTTTGTCCAAAGCTGGATTACCACCTCCTTTTAATGTTATTCTCGTGTGCTACTCACTATTTGAAAGACACAGTGCACAGCAGAAAGATGATCGTAAAATTCTGAAACGGTGGCAATGGAGTTGTGTGCTTATGGACGAGGCACATGCTTTGAAGGACAAAAATAGCTACAGGTGGAAAAACCTAATGTCTGTTGCACGAAGTGCAAACCAGCGTCTAATGCTGACAGGGACACCTCTACAAAATGATTTGCATGAGCTTTGGTCTATGCTGGAGTTTATGATGCCAGATCTTTTCACTACAGAGGATGTGGACTTGAAAAAGCTGTTAAGTGGTGAAGATAGAGATTTGATTGGCCGCATGAAATCCATATTAGGACCCTTTATTTTGAGACGCTTAAAATCTGATGTTATGCAACAACTTGTTCCAAAAATACAACGGGTTGAGTATGTTGTCATGGAAAAGAAGCAAGATGACGCCTATAAAGAAGCCATCGAGGAGTATCGGGCAGCTTCACGAACTCGTATTGCAAAAACTTCAGAGGTTAACTCGAACAGTATTTTTAAGGTTATTCCTCGGCGGCAGATCTCCAACTATTTCGTTCAGTTTCGTAAGATTGCAAATCATCCTTTACTAGTAAGGCGTATTTACAGTGATGAGGATGTGGTTCGTTTTGCCAGAAAGTTACATCCAATGGGTGCATTTGGCTATGAATGTACCTTAGACAAAGTAATTGGGGAACTTAAGAGTTATAGTGACTTCTCTATCCATCGGCTTTTGCTGTGTTATGGTGTCACTGATAAGAAAGGATTTCTTCCGGACAAATATGCATTGCGTTCTGCAAAATCTCAGGCATTGGCTGAACTTCTTCCTTCACTGAAGCAAGCTGGGCATCGTGTGTTGATTTTCAGCCAGTGGACGTCCATGCTTGATATTTTGGAGTGGACTTTGGATGTGATAGGTGTTACATACAGACGCCTTGATGGAAGCACTCAGGTGACGGAAAGACAGACGATAGTTGATACATTCAATAATGATACTTCTATATTTGCATGCTTGCTGTCCACAAGAGCTGGAGGCCAGGGCTTGAACTTGATTGGAGCTGATACCGTAGTCATACATGATATGGATTTCAACCCGCAAATTGACCGACAGGCTGAAGATCGTTGTCATCGCATTGGCCAAGTGAAGCCTGTTACCATATACAGGCTAGTGACTAAGGGTACAGTTGATGAAAATGTCTACGAGATTGCGAAAAGGAAGTTAGTGCTAGATGCTGCAGTCCTGGAATCTGGTGTGGAGATGGATAATGAAGGTGACACATCCACCATGGGAGAGATATTATCGAAACTTTTACTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000729 GO:0000785 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006325 GO:0006338 GO:0006355 GO:0006464 GO:0006476 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0008150 GO:0008152 GO:0009117 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010225 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016363 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0019637 GO:0022402 GO:0022607 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0033554 GO:0034399 GO:0034641 GO:0034644 GO:0035601 GO:0035861 GO:0036211 GO:0043044 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043596 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045893 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051259 GO:0051260 GO:0051276 GO:0051304 GO:0051716 GO:0055086 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070932 GO:0070933 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098732 GO:0104004 GO:1901360 GO:1901363 GO:1901564 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

747

Amino Acids

84.82

Weight (kDa)

5.11

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 198 - 371 2.1e-06 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 201 - 502 3.3e-64 SNF2-related domain
DEAD PF00270 222 - 374 5.8e-06 DEAD/DEAH box helicase
Helicase_C PF00271 574 - 686 1.1e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02090
fragaria_vesca FvH4_4g36060
malus_domestica MD16G1009400.v1.1
prunus_persica Prupe.1G341200_v2.0.a1 Prupe.1G341200_v2.0.a1
pyrus_communis pycom16g00910
rosa_chinensis RchiOBHm_Chr4g0445681
rosa_laevigata RLG00000005717
rosa_multiflora Rmu_sc0003637.1_g000018
rosa_roxburghii Rroxscaffold_5G00386160
rosa_rugosa Rorug04G0364700
rosa_samantha Rh4AG425600
rosa_wichuraiana Rw4G036460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1799
AccB1I GGYRCC 1 cut(s) 810
AccI GTMKAC 2 cut(s) 384, 2118
AccII CGCG 3 cut(s) 9, 78, 109
AccIII TCCGGA 1 cut(s) 1693
AcoI YGGCCR 3 cut(s) 298, 1231, 2056
AcsI RAATTY 1 cut(s) 975
AcuI CTGAAG 3 cut(s) 1398, 1775, 2055
AcyI GRCGYC 4 cut(s) 261, 1353, 1796, 1852
AdeI CACNNNGTG 1 cut(s) 1079
AfaI GTAC 5 cut(s) 500, 602, 710, 1594, 2101
AfiI CCNNNNNNNGG 2 cut(s) 525, 2082
AflII CTTAAG 1 cut(s) 1619
AhlI ACTAGT 1 cut(s) 1510
AjnI CCWGG 2 cut(s) 1956, 2160
AleI CACNNNNGTG 1 cut(s) 1871
AloI GAACNNNNNNTCC 2 cut(s) 866, 898
Alw21I GWGCWC 3 cut(s) 54, 465, 955
Alw26I GTCTC 2 cut(s) 686, 1261
Alw44I GTGCAC 1 cut(s) 951
Ama87I CYCGRG 2 cut(s) 683, 742
Aor13HI TCCGGA 1 cut(s) 1693
AoxI GGCC 4 cut(s) 298, 1231, 1954, 2056
ApaLI GTGCAC 1 cut(s) 951
ApeKI GCWGC 4 cut(s) 515, 845, 1385, 2153
ApoI RAATTY 1 cut(s) 975
Asp700I GAANNNNTTC 1 cut(s) 1743
AspLEI GCGC 2 cut(s) 9, 111
AspS9I GGNCC 3 cut(s) 271, 746, 1253
AsuC2I CCSGG 3 cut(s) 275, 743, 744
AsuHPI GGTGA 3 cut(s) 1223, 1885, 2204
AsuII TTCGAA 1 cut(s) 24
AvaI CYCGRG 2 cut(s) 683, 742
AvaII GGWCC 3 cut(s) 271, 746, 1253
BaeGI GKGCMC 2 cut(s) 815, 955
BaeI ACNNNNGTAYC 2 cut(s) 1974, 2007
BalI TGGCCA 1 cut(s) 2058
BanI GGYRCC 1 cut(s) 810
BanII GRGCYC 1 cut(s) 465
BauI CACGAG 1 cut(s) 920
BbsI GAAGAC 2 cut(s) 180, 695
Bbv12I GWGCWC 3 cut(s) 54, 465, 955
BbvI GCAGC 4 cut(s) 502, 832, 1397, 2140
BccI CCATC 6 cut(s) 326, 670, 1376, 1653, 1853, 2173
BceAI ACGGC 2 cut(s) 285, 583
BcgI CGANNNNNNTGC 2 cut(s) 1004, 1038
BciT130I CCWGG 2 cut(s) 1958, 2162
BcnI CCSGG 3 cut(s) 275, 743, 744
BcoDI GTCTC 2 cut(s) 686, 1261
BcuI ACTAGT 1 cut(s) 1510
BfaI CTAG 3 cut(s) 1511, 2087, 2147
BfmI CTRYAG 3 cut(s) 1045, 1176, 2154
BfrI CTTAAG 1 cut(s) 1619
BglI GCCNNNNNGGC 1 cut(s) 665
BglII AGATCT 2 cut(s) 1165, 1461
BisI GCNGC 7 cut(s) 107, 516, 846, 1234, 1386, 1457, 2154
BlsI GCNGC 7 cut(s) 108, 517, 847, 1235, 1387, 1458, 2155
Bme1390I CCNGG 5 cut(s) 275, 743, 744, 1958, 2162
Bme18I GGWCC 3 cut(s) 271, 746, 1253
BmeT110I CYCGRG 2 cut(s) 683, 742
BmgT120I GGNCC 3 cut(s) 271, 746, 1253
BmiI GGNNCC 6 cut(s) 273, 480, 658, 747, 812, 1255
BmrFI CCNGG 5 cut(s) 275, 743, 744, 1958, 2162
BmrI ACTGGG 1 cut(s) 52
BmsI GCATC 4 cut(s) 367, 1149, 1777, 2140
BmuI ACTGGG 1 cut(s) 52
BoxI GACNNNNGTC 1 cut(s) 744
BpiI GAAGAC 2 cut(s) 180, 695
BplI GAGNNNNNCTC 8 cut(s) 38, 70, 41, 73, 860, 892, 1409, 1441
BpmI CTGGAG 4 cut(s) 227, 815, 1169, 1971
Bpu14I TTCGAA 1 cut(s) 24
BpuEI CTTGAG 2 cut(s) 68, 578
BpuMI CCSGG 3 cut(s) 275, 743, 744
BsaHI GRCGYC 4 cut(s) 261, 1353, 1796, 1852
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 5 cut(s) 466, 742, 1451, 1957, 2205
BsaWI WCCGGW 1 cut(s) 1693
Bsc4I CCNNNNNNNGG 2 cut(s) 525, 2082
Bse1I ACTGG 5 cut(s) 47, 210, 604, 832, 1789
Bse3DI GCAATG 2 cut(s) 998, 1706
BseAI TCCGGA 1 cut(s) 1693
BseBI CCWGG 2 cut(s) 1958, 2162
BseDI CCNNGG 5 cut(s) 466, 742, 1451, 1957, 2205
BseLI CCNNNNNNNGG 2 cut(s) 525, 2082
BseMI GCAATG 2 cut(s) 998, 1706
BseMII CTCAG 3 cut(s) 558, 1739, 1883
BseNI ACTGG 5 cut(s) 47, 210, 604, 832, 1789
BseRI GAGGAG 4 cut(s) 46, 62, 386, 1388
BseSI GKGCMC 2 cut(s) 815, 955
BseXI GCAGC 4 cut(s) 502, 832, 1397, 2140
BseYI CCCAGC 1 cut(s) 1763
Bsh1236I CGCG 3 cut(s) 9, 78, 109
BshFI GGCC 4 cut(s) 300, 1233, 1956, 2058
BshNI GGYRCC 1 cut(s) 810
BsiHKAI GWGCWC 3 cut(s) 54, 465, 955
BsiHKCI CYCGRG 2 cut(s) 683, 742
BsiSI CCGG 4 cut(s) 275, 482, 743, 1694
BslFI GGGAC 2 cut(s) 257, 1120
BslI CCNNNNNNNGG 2 cut(s) 525, 2082
BsmAI GTCTC 2 cut(s) 686, 1261
BsmBI CGTCTC 1 cut(s) 1261
BsmFI GGGAC 2 cut(s) 257, 1120
BsnI GGCC 4 cut(s) 300, 1233, 1956, 2058
Bso31I GGTCTC 1 cut(s) 686
BsoBI CYCGRG 2 cut(s) 683, 742
Bsp119I TTCGAA 1 cut(s) 24
Bsp1286I GDGCHC 4 cut(s) 54, 465, 815, 955
Bsp13I TCCGGA 1 cut(s) 1693
Bsp143I GATC 6 cut(s) 134, 851, 967, 1165, 1461, 2038
Bsp19I CCATGG 1 cut(s) 2205
BspANI GGCC 4 cut(s) 300, 1233, 1956, 2058
BspCNI CTCAG 3 cut(s) 559, 1738, 1882
BspEI TCCGGA 1 cut(s) 1693
BspFNI CGCG 3 cut(s) 9, 78, 109
BspLI GGNNCC 6 cut(s) 273, 480, 658, 747, 812, 1255
BspMAI CTGCAG 1 cut(s) 2158
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 810
BspTI CTTAAG 1 cut(s) 1619
BspTNI GGTCTC 1 cut(s) 686
BsrDI GCAATG 2 cut(s) 998, 1706
BsrI ACTGG 5 cut(s) 47, 210, 604, 832, 1789
BssECI CCNNGG 5 cut(s) 466, 742, 1451, 1957, 2205
BssMI GATC 6 cut(s) 134, 851, 967, 1165, 1461, 2038
BssNI GRCGYC 4 cut(s) 261, 1353, 1796, 1852
BssSI CACGAG 1 cut(s) 920
BssT1I CCWWGG 1 cut(s) 2205
Bst2BI CACGAG 1 cut(s) 920
Bst2UI CCWGG 2 cut(s) 1958, 2162
Bst4CI ACNGT 8 cut(s) 289, 826, 950, 987, 1433, 1529, 1987, 2104
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 4 cut(s) 261, 1353, 1796, 1852
BstAFI CTTAAG 1 cut(s) 1619
BstAPI GCANNNNNTGC 1 cut(s) 1079
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 3 cut(s) 1761, 1930, 1934
BstDEI CTNAG 5 cut(s) 567, 1597, 1725, 1869, 2094
BstDSI CCRYGG 2 cut(s) 466, 2205
BstENI CCTNNNNNAGG 1 cut(s) 523
BstFNI CGCG 3 cut(s) 9, 78, 109
BstHHI GCGC 2 cut(s) 9, 111
BstKTI GATC 6 cut(s) 137, 854, 970, 1168, 1464, 2041
BstMAI GTCTC 2 cut(s) 686, 1261
BstMBI GATC 6 cut(s) 134, 851, 967, 1165, 1461, 2038
BstMWI GCNNNNNNNGC 5 cut(s) 665, 854, 1079, 1096, 1765
BstNI CCWGG 2 cut(s) 1958, 2162
BstNSI RCATGY 3 cut(s) 561, 1025, 1932
BstPAI GACNNNNGTC 1 cut(s) 744
BstSCI CCNGG 5 cut(s) 273, 741, 742, 1956, 2160
BstSFI CTRYAG 3 cut(s) 1045, 1176, 2154
BstSLI GKGCMC 2 cut(s) 815, 955
BstUI CGCG 3 cut(s) 9, 78, 109
BstV1I GCAGC 4 cut(s) 502, 832, 1397, 2140
BstV2I GAAGAC 2 cut(s) 180, 695
BstX2I RGATCY 2 cut(s) 1165, 1461
BstXI CCANNNNNNTGG 2 cut(s) 611, 839
BstYI RGATCY 2 cut(s) 1165, 1461
BsuRI GGCC 4 cut(s) 300, 1233, 1956, 2058
BtgI CCRYGG 2 cut(s) 466, 2205
BtgZI GCGATG 2 cut(s) 345, 2033
BtsIMutI CAGTG 6 cut(s) 822, 955, 1534, 1671, 1751, 1796
Cac8I GCNNGC 3 cut(s) 1761, 1930, 1934
CfoI GCGC 2 cut(s) 9, 111
Cfr13I GGNCC 3 cut(s) 271, 746, 1253
Cfr9I CCCGGG 1 cut(s) 742
CseI GACGC 7 cut(s) 67, 175, 269, 1079, 1278, 1361, 1860
Csp6I GTAC 5 cut(s) 499, 601, 709, 1593, 2100
CviQI GTAC 5 cut(s) 499, 601, 709, 1593, 2100
DdeI CTNAG 5 cut(s) 567, 1597, 1725, 1869, 2094
DpnI GATC 6 cut(s) 136, 853, 969, 1167, 1463, 2040
DpnII GATC 6 cut(s) 134, 851, 967, 1165, 1461, 2038
DraI TTTAAA 1 cut(s) 732
DraIII CACNNNGTG 1 cut(s) 1079
EaeI YGGCCR 3 cut(s) 298, 1231, 2056
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
EciI GGCGGA 3 cut(s) 49, 461, 669
Ecl136II GAGCTC 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 2205
Eco24I GRGCYC 1 cut(s) 465
Eco31I GGTCTC 1 cut(s) 686
Eco47I GGWCC 3 cut(s) 271, 746, 1253
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 3 cut(s) 1398, 1775, 2055
Eco88I CYCGRG 2 cut(s) 683, 742
EcoICRI GAGCTC 1 cut(s) 463
EcoNI CCTNNNNNAGG 1 cut(s) 523
EcoO109I RGGNCCY 2 cut(s) 271, 1253
EcoRII CCWGG 2 cut(s) 1956, 2160
EcoT14I CCWWGG 1 cut(s) 2205
EcoT22I ATGCAT 1 cut(s) 1708
EcoT38I GRGCYC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 2205
Esp3I CGTCTC 1 cut(s) 1261
FalI AAGNNNNNCTT 4 cut(s) 841, 873, 1674, 1706
FaqI GGGAC 2 cut(s) 257, 1120
FauI CCCGC 3 cut(s) 2, 83, 2022
FblI GTMKAC 2 cut(s) 384, 2118
Fnu4HI GCNGC 7 cut(s) 107, 516, 846, 1234, 1386, 1457, 2154
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 7 cut(s) 107, 516, 846, 1234, 1386, 1457, 2154
FspBI CTAG 3 cut(s) 1511, 2087, 2147
GlaI GCGC 2 cut(s) 8, 110
GluI GCNGC 7 cut(s) 107, 516, 846, 1234, 1386, 1457, 2154
GsaI CCCAGC 1 cut(s) 1767
GsuI CTGGAG 4 cut(s) 227, 815, 1169, 1971
HaeIII GGCC 4 cut(s) 300, 1233, 1956, 2058
HapII CCGG 4 cut(s) 275, 482, 743, 1694
HgaI GACGC 7 cut(s) 67, 175, 269, 1079, 1278, 1361, 1860
HhaI GCGC 2 cut(s) 9, 111
Hin1I GRCGYC 4 cut(s) 261, 1353, 1796, 1852
Hin6I GCGC 2 cut(s) 7, 109
HinP1I GCGC 2 cut(s) 7, 109
HincII GTYRAC 2 cut(s) 619, 1423
HindII GTYRAC 2 cut(s) 619, 1423
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 7 cut(s) 119, 176, 218, 353, 572, 589, 2165
HpaI GTTAAC 2 cut(s) 619, 1423
HpaII CCGG 4 cut(s) 275, 482, 743, 1694
HphI GGTGA 3 cut(s) 1223, 1885, 2204
Hpy188III TCNNGA 7 cut(s) 85, 190, 232, 539, 685, 1392, 1694
Hpy99I CGWCG 9 cut(s) 20, 38, 167, 191, 260, 263, 269, 306, 478
HpyAV CCTTC 7 cut(s) 73, 133, 139, 637, 1024, 1758, 2183
HpyCH4III ACNGT 8 cut(s) 289, 826, 950, 987, 1433, 1529, 1987, 2104
HpyCH4IV ACGT 2 cut(s) 707, 1796
HpyF10VI GCNNNNNNNGC 5 cut(s) 665, 854, 1079, 1096, 1765
HpyF3I CTNAG 5 cut(s) 567, 1597, 1725, 1869, 2094
HpySE526I ACGT 2 cut(s) 707, 1796
Hsp92I GRCGYC 4 cut(s) 261, 1353, 1796, 1852
HspAI GCGC 2 cut(s) 7, 109
Kpn2I TCCGGA 1 cut(s) 1693
KspAI GTTAAC 2 cut(s) 619, 1423
Kzo9I GATC 6 cut(s) 134, 851, 967, 1165, 1461, 2038
LmnI GCTCC 6 cut(s) 49, 460, 468, 484, 656, 1976
Lsp1109I GCAGC 4 cut(s) 502, 832, 1397, 2140
LweI GCATC 4 cut(s) 367, 1149, 1777, 2140
MaeI CTAG 3 cut(s) 1511, 2087, 2147
MaeII ACGT 2 cut(s) 707, 1796
MaeIII GTNAC 8 cut(s) 1560, 1631, 1669, 1840, 1873, 2071, 2089, 2192
MalI GATC 6 cut(s) 136, 853, 969, 1167, 1463, 2040
MboI GATC 6 cut(s) 134, 851, 967, 1165, 1461, 2038
MflI RGATCY 2 cut(s) 1165, 1461
MhlI GDGCHC 4 cut(s) 54, 465, 815, 955
MlsI TGGCCA 1 cut(s) 2058
MluCI AATT 5 cut(s) 245, 784, 975, 1608, 2019
MluNI TGGCCA 1 cut(s) 2058
MlyI GAGTC 3 cut(s) 128, 170, 347
MmeI TCCRAC 3 cut(s) 57, 372, 1491
Mox20I TGGCCA 1 cut(s) 2058
Mph1103I ATGCAT 1 cut(s) 1708
MroI TCCGGA 1 cut(s) 1693
MroXI GAANNNNTTC 1 cut(s) 1743
MscI TGGCCA 1 cut(s) 2058
MslI CAYNNNNRTG 2 cut(s) 732, 1871
Msp20I TGGCCA 1 cut(s) 2058
MspCI CTTAAG 1 cut(s) 1619
MspI CCGG 4 cut(s) 275, 482, 743, 1694
MspR9I CCNGG 5 cut(s) 275, 743, 744, 1958, 2162
MvaI CCWGG 2 cut(s) 1958, 2162
MvnI CGCG 3 cut(s) 9, 78, 109
MwoI GCNNNNNNNGC 5 cut(s) 665, 854, 1079, 1096, 1765
NciI CCSGG 3 cut(s) 275, 743, 744
NcoI CCATGG 1 cut(s) 2205
NdeII GATC 6 cut(s) 134, 851, 967, 1165, 1461, 2038
NlaIV GGNNCC 6 cut(s) 273, 480, 658, 747, 812, 1255
NmeAIII GCCGAG 1 cut(s) 1432
NmuCI GTSAC 5 cut(s) 1631, 1669, 1873, 2089, 2192
NsiI ATGCAT 1 cut(s) 1708
NspI RCATGY 3 cut(s) 561, 1025, 1932
NspV TTCGAA 1 cut(s) 24
OliI CACNNNNGTG 1 cut(s) 1871
PaeI GCATGC 1 cut(s) 1932
PcsI WCGNNNNNNNCGW 5 cut(s) 21, 30, 261, 264, 327
PdmI GAANNNNTTC 1 cut(s) 1743
PfeI GAWTC 4 cut(s) 218, 572, 589, 2165
PflFI GACNNNGTC 1 cut(s) 269
PfoI TCCNGGA 2 cut(s) 273, 2160
PkrI GCNGC 7 cut(s) 108, 517, 847, 1235, 1387, 1458, 2155
PleI GAGTC 3 cut(s) 127, 170, 347
PpsI GAGTC 3 cut(s) 127, 170, 347
PpuMI RGGWCCY 2 cut(s) 271, 1253
PshAI GACNNNNGTC 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 465
Psp5II RGGWCCY 2 cut(s) 271, 1253
Psp6I CCWGG 2 cut(s) 1956, 2160
PspFI CCCAGC 1 cut(s) 1763
PspGI CCWGG 2 cut(s) 1956, 2160
PspN4I GGNNCC 6 cut(s) 273, 480, 658, 747, 812, 1255
PspPI GGNCC 3 cut(s) 271, 746, 1253
PspPPI RGGWCCY 2 cut(s) 271, 1253
PstI CTGCAG 1 cut(s) 2158
PsuI RGATCY 2 cut(s) 1165, 1461
PsyI GACNNNGTC 1 cut(s) 269
RsaI GTAC 5 cut(s) 500, 602, 710, 1594, 2101
RsaNI GTAC 5 cut(s) 499, 601, 709, 1593, 2100
RseI CAYNNNNRTG 2 cut(s) 732, 1871
SacI GAGCTC 1 cut(s) 465
SatI GCNGC 7 cut(s) 107, 516, 846, 1234, 1386, 1457, 2154
Sau3AI GATC 6 cut(s) 134, 851, 967, 1165, 1461, 2038
Sau96I GGNCC 3 cut(s) 271, 746, 1253
SchI GAGTC 3 cut(s) 128, 170, 347
ScrFI CCNGG 5 cut(s) 275, 743, 744, 1958, 2162
SduI GDGCHC 4 cut(s) 54, 465, 815, 955
SfaNI GCATC 4 cut(s) 367, 1149, 1777, 2140
SfcI CTRYAG 3 cut(s) 1045, 1176, 2154
SfuI TTCGAA 1 cut(s) 24
SinI GGWCC 3 cut(s) 271, 746, 1253
SmaI CCCGGG 1 cut(s) 744
SmiI ATTTAAAT 1 cut(s) 732
SmiMI CAYNNNNRTG 2 cut(s) 732, 1871
SmlI CTYRAG 3 cut(s) 83, 593, 1619
SmoI CTYRAG 3 cut(s) 83, 593, 1619
SpeI ACTAGT 1 cut(s) 1510
SphI GCATGC 1 cut(s) 1932
Sse9I AATT 5 cut(s) 245, 784, 975, 1608, 2019
SspI AATATT 1 cut(s) 349
SspMI CTAG 3 cut(s) 1511, 2087, 2147
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 5 cut(s) 273, 741, 742, 1956, 2160
StyI CCWWGG 1 cut(s) 2205
SwaI ATTTAAAT 1 cut(s) 732
TaaI ACNGT 8 cut(s) 289, 826, 950, 987, 1433, 1529, 1987, 2104
TaiI ACGT 2 cut(s) 710, 1799
TaqI TCGA 8 cut(s) 15, 24, 117, 189, 315, 1371, 1427, 2222
TaqII GACCGA 1 cut(s) 2040
TasI AATT 5 cut(s) 245, 784, 975, 1608, 2019
TauI GCSGC 3 cut(s) 109, 1236, 1459
TfiI GAWTC 4 cut(s) 218, 572, 589, 2165
TscAI CASTG 6 cut(s) 829, 955, 1534, 1678, 1758, 1796
TseFI GTSAC 5 cut(s) 1631, 1669, 1873, 2089, 2192
TseI GCWGC 4 cut(s) 515, 845, 1385, 2153
Tsp45I GTSAC 5 cut(s) 1631, 1669, 1873, 2089, 2192
TspDTI ATGAA 6 cut(s) 17, 186, 1253, 1602, 2124, 2202
TspGWI ACGGA 1 cut(s) 1892
TspMI CCCGGG 1 cut(s) 742
TspRI CASTG 6 cut(s) 829, 955, 1534, 1678, 1758, 1796
Tth111I GACNNNGTC 1 cut(s) 269
Vha464I CTTAAG 1 cut(s) 1619
VneI GTGCAC 1 cut(s) 951
VpaK11BI GGWCC 3 cut(s) 271, 746, 1253
XagI CCTNNNNNAGG 1 cut(s) 523
XapI RAATTY 1 cut(s) 975
XceI RCATGY 3 cut(s) 561, 1025, 1932
XmaI CCCGGG 1 cut(s) 742
XmiI GTMKAC 2 cut(s) 384, 2118
XmnI GAANNNNTTC 1 cut(s) 1743
XspI CTAG 3 cut(s) 1511, 2087, 2147
ZraI GACGTC 1 cut(s) 1797
Zsp2I ATGCAT 1 cut(s) 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.