MD16G1010000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012271
Physical Location & Seq
Forward (+)
780085 .. 782332
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1010000.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCGATACCGGTGGAGGCGGCGGCGGCGGAAGTAGAAAGAGAGAAAGAGATGGAGTCGACTAGGAAAGAGGAGATGGCGCCATGGGAGCAACACTCGACTGTAATCAGCATCCCTCGCTTCGACTACAACGCCCCTTCTTCTCTTCTCCAACGCTCCCACTCCGGCTTCCTCATTACCTGCCCCATCAGTCTGGAGAAAAGCGCCACAAAAGAAGCAATTTCCCTCCTTGCAAAGTATGTTCAGTGCTTTAATAGTTGCAGCTCAGAGGACAATACCAGTTCGAAAAGAAGGAAGCTTTCGAAAGAGGATATCGATAGCGAGTGCTGCAATGGCGGCGGGGAAAGCAAAGATGCTGCTACTGCTAACTCTGAGGACGCTCGTGATGATCTTCAGGAGCGTACTCCTCGTTTGTCTCCTGCAAAGGTCGATGCGGAAGGAGATGCAAGTCCACTTCTTTCGCTGGTTAAGTTGACAAAGAGTGGTCTGCTTCTCTTTACTTTCCGCAGAAACACTTCTCCTGACGTTGTTGATACTGTATCGAAAATTATTCAGGCTGTGGAATCCAAGAGTGTTGGTTCACCACTTTGGTGTAATCGCATTTTCCCTATCCAAGCTACTTGTAGTTTGAAAGAGAAGGAACTGCATGAAGTTGTATCAAGGCTTGTTCTTCAATTCGTGAACGATAATCAGAAGCTTGTGCGGCCCATAAAGTTTGCAATTGCATATAATAGAAGAGGGATAGAGACAGAACTGAATATTCCCAAAGAATCTTCAAATGCAATTGATATGTTGGACCGTGACAAATGCTTTGCCGCAGTGGCTACTGCTGTCAAGGGTGTTCTATCAGATTCGGTGGTGGACCTCAAATCTCCGGAACTCTGTGTTCTTCTTGAGCTACTACCCCTTTCTGGTGTACCCAAAGAGTCGTTAGTGGTTGCTGTATCAGTTCTTCCTCGATACATTGTTAATGCAAAGCCGAAACTATGCATCAAGGCACTGGTTTCCGATACAAAAACGAAGGGGAAAAAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
THUMP

Protein Analysis

346

Amino Acids

37.87

Weight (kDa)

7.47

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09290
fragaria_vesca FvH4_4g35990
malus_domestica MD16G1010000.v1.1
prunus_persica Prupe.1G340600_v2.0.a1 Prupe.1G340600_v2.0.a1 Prupe.1G340600_v2.0.a1
pyrus_communis pycom16g00980
rosa_chinensis RchiOBHm_Chr4g0445621
rosa_laevigata RLG00000005724
rosa_multiflora Rmu_sc0019383.1_g000001
rosa_roxburghii Rroxscaffold_5G00386100
rosa_rugosa Rorug04G0363100 Rorug04G0363200
rosa_samantha Rh4AG424600 Rh4AG425000 Rh4BG436800 Rh4CG451300 Rh4DG433300
rosa_wichuraiana Rw4G036400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 79
AccI GTMKAC 1 cut(s) 59
AccIII TCCGGA 1 cut(s) 874
AcuI CTGAAG 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 2 cut(s) 403, 918
AfiI CCNNNNNNNGG 1 cut(s) 911
AgeI ACCGGT 1 cut(s) 10
AgsI TTSAA 3 cut(s) 631, 674, 777
AleI CACNNNNGTG 1 cut(s) 589
AluBI AGCT 5 cut(s) 264, 298, 617, 697, 898
AluI AGCT 5 cut(s) 264, 298, 617, 697, 898
Alw26I GTCTC 2 cut(s) 420, 740
Aor13HI TCCGGA 1 cut(s) 874
AoxI GGCC 1 cut(s) 704
ApeKI GCWGC 3 cut(s) 261, 327, 356
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
AsiGI ACCGGT 1 cut(s) 10
Asp700I GAANNNNTTC 1 cut(s) 514
AspLEI GCGC 2 cut(s) 82, 206
AspS9I GGNCC 3 cut(s) 705, 796, 862
AsuHPI GGTGA 1 cut(s) 573
AsuII TTCGAA 2 cut(s) 284, 302
AvaII GGWCC 2 cut(s) 796, 862
BanI GGYRCC 1 cut(s) 79
BarI GAAGNNNNNNTAC 2 cut(s) 936, 968
BauI CACGAG 1 cut(s) 381
BbvI GCAGC 3 cut(s) 273, 314, 343
BccI CCATC 3 cut(s) 46, 70, 194
BcoDI GTCTC 2 cut(s) 420, 740
BfaI CTAG 1 cut(s) 63
BfoI RGCGCY 2 cut(s) 83, 207
BfuAI ACCTGC 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 821
BisI GCNGC 9 cut(s) 21, 24, 27, 262, 328, 337, 357, 704, 816
BlsI GCNGC 9 cut(s) 22, 25, 28, 263, 329, 338, 358, 705, 817
Bme18I GGWCC 2 cut(s) 796, 862
BmgT120I GGNCC 3 cut(s) 705, 796, 862
BmiI GGNNCC 1 cut(s) 81
BmsI GCATC 5 cut(s) 120, 343, 421, 433, 999
BplI GAGNNNNNCTC 2 cut(s) 80, 112
BpmI CTGGAG 1 cut(s) 215
Bpu14I TTCGAA 2 cut(s) 284, 302
BpuEI CTTGAG 1 cut(s) 914
Bsa29I ATCGAT 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 83
BsaWI WCCGGW 2 cut(s) 10, 874
Bsc4I CCNNNNNNNGG 1 cut(s) 911
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 2 cut(s) 279, 1005
Bse3DI GCAATG 1 cut(s) 337
BseAI TCCGGA 1 cut(s) 874
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 83
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 911
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 279, 363
BseNI ACTGG 2 cut(s) 279, 1005
BseRI GAGGAG 2 cut(s) 86, 396
BseXI GCAGC 3 cut(s) 273, 314, 343
BshFI GGCC 1 cut(s) 706
BshNI GGYRCC 1 cut(s) 79
BshTI ACCGGT 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 315
BsiSI CCGG 3 cut(s) 11, 165, 875
BslI CCNNNNNNNGG 1 cut(s) 911
BsmAI GTCTC 2 cut(s) 420, 740
BsnI GGCC 1 cut(s) 706
Bsp119I TTCGAA 2 cut(s) 284, 302
Bsp13I TCCGGA 1 cut(s) 874
Bsp143I GATC 1 cut(s) 388
Bsp19I CCATGG 1 cut(s) 83
BspANI GGCC 1 cut(s) 706
BspCNI CTCAG 2 cut(s) 278, 364
BspDI ATCGAT 1 cut(s) 315
BspEI TCCGGA 1 cut(s) 874
BspLI GGNNCC 1 cut(s) 81
BspMI ACCTGC 1 cut(s) 188
BspT104I TTCGAA 2 cut(s) 284, 302
BspT107I GGYRCC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 2 cut(s) 279, 1005
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 83
BssMI GATC 1 cut(s) 388
BssNI GRCGYC 1 cut(s) 80
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 83
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 3 cut(s) 103, 538, 800
Bst6I CTCTTC 2 cut(s) 150, 730
BstACI GRCGYC 1 cut(s) 80
BstBI TTCGAA 2 cut(s) 284, 302
BstDEI CTNAG 2 cut(s) 265, 372
BstDSI CCRYGG 1 cut(s) 83
BstF5I GGATG 1 cut(s) 111
BstH2I RGCGCY 2 cut(s) 83, 207
BstHHI GCGC 2 cut(s) 82, 206
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 2 cut(s) 420, 740
BstMBI GATC 1 cut(s) 388
BstV1I GCAGC 3 cut(s) 273, 314, 343
BstXI CCANNNNNNTGG 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 315
BsuRI GGCC 1 cut(s) 706
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 3 cut(s) 251, 825, 998
BveI ACCTGC 1 cut(s) 188
CfoI GCGC 2 cut(s) 82, 206
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 3 cut(s) 705, 796, 862
ClaI ATCGAT 1 cut(s) 315
CseI GACGC 1 cut(s) 386
Csp6I GTAC 2 cut(s) 402, 917
CspAI ACCGGT 1 cut(s) 10
CviAII CATG 2 cut(s) 84, 647
CviQI GTAC 2 cut(s) 402, 917
DdeI CTNAG 2 cut(s) 265, 372
DinI GGCGCC 1 cut(s) 81
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
Eam1104I CTCTTC 2 cut(s) 150, 730
EarI CTCTTC 2 cut(s) 150, 730
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 83
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 796, 862
Eco57I CTGAAG 1 cut(s) 377
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 83
EcoT22I ATGCAT 1 cut(s) 992
EgeI GGCGCC 1 cut(s) 81
EheI GGCGCC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 83
FaeI CATG 2 cut(s) 87, 650
FaiI YATR 8 cut(s) 85, 240, 648, 710, 727, 729, 791, 988
FatI CATG 2 cut(s) 83, 646
FauI CCCGC 1 cut(s) 332
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 9 cut(s) 21, 24, 27, 262, 328, 337, 357, 704, 816
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 9 cut(s) 21, 24, 27, 262, 328, 337, 357, 704, 816
FspBI CTAG 1 cut(s) 63
GlaI GCGC 2 cut(s) 81, 205
GluI GCNGC 9 cut(s) 21, 24, 27, 262, 328, 337, 357, 704, 816
GsuI CTGGAG 1 cut(s) 215
HaeII RGCGCY 2 cut(s) 83, 207
HaeIII GGCC 1 cut(s) 706
HapII CCGG 3 cut(s) 11, 165, 875
HgaI GACGC 1 cut(s) 386
HhaI GCGC 2 cut(s) 82, 206
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 2 cut(s) 87, 650
Hin6I GCGC 2 cut(s) 80, 204
HinP1I GCGC 2 cut(s) 80, 204
HincII GTYRAC 2 cut(s) 60, 474
HindII GTYRAC 2 cut(s) 60, 474
HindIII AAGCTT 2 cut(s) 296, 695
HinfI GANTC 5 cut(s) 56, 563, 770, 851, 926
HpaII CCGG 3 cut(s) 11, 165, 875
HphI GGTGA 1 cut(s) 573
Hpy166II GTNNAC 7 cut(s) 60, 452, 474, 581, 682, 862, 917
Hpy188I TCNGA 5 cut(s) 268, 373, 693, 850, 1009
Hpy188III TCNNGA 7 cut(s) 194, 383, 395, 521, 679, 875, 893
Hpy8I GTNNAC 7 cut(s) 60, 452, 474, 581, 682, 862, 917
HpyAV CCTTC 5 cut(s) 147, 285, 431, 631, 1015
HpyCH4III ACNGT 3 cut(s) 103, 538, 800
HpyCH4IV ACGT 1 cut(s) 525
HpyF3I CTNAG 2 cut(s) 265, 372
HpySE526I ACGT 1 cut(s) 525
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 2 cut(s) 87, 650
HspAI GCGC 2 cut(s) 80, 204
KasI GGCGCC 1 cut(s) 79
Kpn2I TCCGGA 1 cut(s) 874
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 3 cut(s) 88, 161, 397
Lsp1109I GCAGC 3 cut(s) 273, 314, 343
LweI GCATC 5 cut(s) 120, 343, 421, 433, 999
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 1 cut(s) 800
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 8 cut(s) 132, 137, 383, 662, 747, 765, 881, 944
MfeI CAATTG 2 cut(s) 720, 783
MluCI AATT 5 cut(s) 219, 546, 674, 720, 783
Mly113I GGCGCC 1 cut(s) 80
MlyI GAGTC 2 cut(s) 65, 935
MmeI TCCRAC 2 cut(s) 175, 774
Mph1103I ATGCAT 1 cut(s) 992
MroI TCCGGA 1 cut(s) 874
MroXI GAANNNNTTC 1 cut(s) 514
MseI TTAA 3 cut(s) 252, 468, 969
MslI CAYNNNNRTG 1 cut(s) 589
MspI CCGG 3 cut(s) 11, 165, 875
MunI CAATTG 2 cut(s) 720, 783
NarI GGCGCC 1 cut(s) 80
NcoI CCATGG 1 cut(s) 83
NdeII GATC 1 cut(s) 388
NlaIII CATG 2 cut(s) 87, 650
NlaIV GGNNCC 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 800
NsiI ATGCAT 1 cut(s) 992
NspV TTCGAA 2 cut(s) 284, 302
OliI CACNNNNGTG 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 514
PfeI GAWTC 3 cut(s) 563, 770, 851
PinAI ACCGGT 1 cut(s) 10
PkrI GCNGC 9 cut(s) 22, 25, 28, 263, 329, 338, 358, 705, 817
PleI GAGTC 2 cut(s) 64, 934
PluTI GGCGCC 1 cut(s) 83
PpsI GAGTC 2 cut(s) 64, 934
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 3 cut(s) 705, 796, 862
RsaI GTAC 2 cut(s) 403, 918
RsaNI GTAC 2 cut(s) 402, 917
RseI CAYNNNNRTG 1 cut(s) 589
SalI GTCGAC 1 cut(s) 58
SaqAI TTAA 3 cut(s) 252, 468, 969
SatI GCNGC 9 cut(s) 21, 24, 27, 262, 328, 337, 357, 704, 816
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 3 cut(s) 705, 796, 862
SchI GAGTC 2 cut(s) 65, 935
SetI ASST 9 cut(s) 182, 266, 300, 429, 528, 619, 699, 867, 900
SfaNI GCATC 5 cut(s) 120, 343, 421, 433, 999
SfoI GGCGCC 1 cut(s) 81
SfuI TTCGAA 2 cut(s) 284, 302
SinI GGWCC 2 cut(s) 796, 862
SmiMI CAYNNNNRTG 1 cut(s) 589
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
Sse9I AATT 5 cut(s) 219, 546, 674, 720, 783
SspDI GGCGCC 1 cut(s) 79
SspI AATATT 1 cut(s) 760
SspMI CTAG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 3 cut(s) 103, 538, 800
TaiI ACGT 1 cut(s) 528
TaqI TCGA 9 cut(s) 59, 98, 123, 284, 302, 315, 429, 542, 958
TasI AATT 5 cut(s) 219, 546, 674, 720, 783
TauI GCSGC 6 cut(s) 23, 26, 29, 339, 706, 818
TfiI GAWTC 3 cut(s) 563, 770, 851
Tru1I TTAA 3 cut(s) 252, 468, 969
Tru9I TTAA 3 cut(s) 252, 468, 969
TscAI CASTG 3 cut(s) 251, 825, 1005
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 3 cut(s) 261, 327, 356
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 1 cut(s) 663
TspRI CASTG 3 cut(s) 251, 825, 1005
VpaK11BI GGWCC 2 cut(s) 796, 862
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 514
XspI CTAG 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.