Rh4CG451300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012271
Physical Location & Seq
Reverse (-)
68461404 .. 68464018
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG451300.1

Sequence Viewer

Length: 984 bp
ATGGCGGTGGCGGACTGCGGAAGAGAGATAGAAACAGAGCTGAGCAAGAAGAAGGAAATGACGCCATGGGAGCAGCACTCTGCCGTCATCAGCATCCCTCGCTTCGACTACAACGCCCCTTCGTCTCTTCTTCATCACTCCCACTCCGGCTTCCTCATCACCTGCCCCATCAAGCGGGAGAAAAGCGCCACTAAAGAAGCAATCTCCCTCTTTGCAAAGTATATTCAGTGCTGTAATAGTAGCGGTAGTGAAGAAAATTCGGCTTCTAAAAGAAGGAAGCTTTCGGAGGAAAATGTGGATGGTGAGGATAGAGTGAATGATAATGATGTGGCAGGTGGTGATGTTGAAGAAGGTGCTTGCTCTCATGTGATGGTGGATACAGATGGAGAGACAGGTCCTCTTCTTAAGCTAGTTAAGCTGACAAAGAGTGGTTTGCTTCTCTTTATTTTCGGCAAAAATGTTTCTCCTGATGTCGTTGGGATTGTTTCAAAGATTGTACAGTCTGTGGAATCCGGGAGCTCTGGTTCACTTCTTTGGTGTAATCGTATTTTCCCTATCCAAGCTACTTGTAGTTTGAATGAAAAGGAACTGCGTGAAGTTGTATCAAATCTTGTGCTTCAATTTATGAAAGATAATCAAAAACTTGTGCGGCCCATAAAGTTTGCAATTGGGTATAACAGAAGAGGAATAGAGACCGAATTGAACATTCCAAAAGAAGCCCCAAATGCACTCGGGTTGTTGGACCGTAACAAATGCTTTGCTTTAGTGGCTTCTGCAGTCAAAGATGTTGTATCGGATTCAGTGGTGGATCTCAAAGCTCCAGAGCTCTCTGTTCTTATTGAGCTACTGCCCCTCTCTGGTATACCAAAAGGGTCTTTAGTGGTTGCTGTATCAGTTCTTCCTCAAAACGTTGTTAGTGCAAAGCCAAAACTATCCATCAAGGCACTGATTTCTGATACCAGGACAAAGGGTGAAAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
THUMP

Protein Analysis

327

Amino Acids

35.46

Weight (kDa)

6.96

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09290
fragaria_vesca FvH4_4g35990
malus_domestica MD16G1010000.v1.1
prunus_persica Prupe.1G340600_v2.0.a1 Prupe.1G340600_v2.0.a1 Prupe.1G340600_v2.0.a1
pyrus_communis pycom16g00980
rosa_chinensis RchiOBHm_Chr4g0445621
rosa_laevigata RLG00000005724
rosa_multiflora Rmu_sc0019383.1_g000001
rosa_roxburghii Rroxscaffold_5G00386100
rosa_rugosa Rorug04G0363100 Rorug04G0363200
rosa_samantha Rh4AG424600 Rh4AG425000 Rh4BG436800 Rh4CG451300 Rh4DG433300
rosa_wichuraiana Rw4G036400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 170, 323
Acc36I ACCTGC 2 cut(s) 170, 323
AccI GTMKAC 1 cut(s) 862
AciI CCGC 6 cut(s) 5, 11, 18, 175, 243, 649
AclI AACGTT 1 cut(s) 909
AclWI GGATC 1 cut(s) 816
AcsI RAATTY 1 cut(s) 256
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 2 cut(s) 174, 857
AflII CTTAAG 1 cut(s) 404
AgsI TTSAA 5 cut(s) 347, 489, 577, 620, 703
AjnI CCWGG 1 cut(s) 959
AloI GAACNNNNNNTCC 2 cut(s) 508, 540
AluBI AGCT 9 cut(s) 40, 280, 409, 418, 519, 563, 818, 826, 844
AluI AGCT 9 cut(s) 40, 280, 409, 418, 519, 563, 818, 826, 844
Alw21I GWGCWC 2 cut(s) 521, 828
Alw26I GTCTC 3 cut(s) 129, 383, 686
AlwI GGATC 1 cut(s) 816
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 1 cut(s) 650
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 1 cut(s) 256
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 3 cut(s) 395, 651, 742
AsuC2I CCSGG 1 cut(s) 514
AsuHPI GGTGA 4 cut(s) 151, 314, 350, 983
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 2 cut(s) 395, 742
BanII GRGCYC 2 cut(s) 521, 828
BarI GAAGNNNNNNTAC 2 cut(s) 882, 914
Bbv12I GWGCWC 2 cut(s) 521, 828
BbvI GCAGC 1 cut(s) 85
BccI CCATC 5 cut(s) 176, 293, 364, 377, 944
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 961
BciVI GTATCC 1 cut(s) 370
BcnI CCSGG 1 cut(s) 514
BcoDI GTCTC 3 cut(s) 129, 383, 686
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 1 cut(s) 774
BfoI RGCGCY 1 cut(s) 189
BfrI CTTAAG 1 cut(s) 404
BfuAI ACCTGC 2 cut(s) 170, 323
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 2 cut(s) 74, 650
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 2 cut(s) 75, 651
Bme1390I CCNGG 2 cut(s) 514, 961
Bme18I GGWCC 2 cut(s) 395, 742
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 3 cut(s) 395, 651, 742
BmrFI CCNGG 2 cut(s) 514, 961
BmsI GCATC 1 cut(s) 102
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpmI CTGGAG 1 cut(s) 804
Bpu1102I GCTNAGC 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 514
BsaHI GRCGYC 1 cut(s) 62
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 1 cut(s) 65
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 508, 538
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 857
BseBI CCWGG 1 cut(s) 961
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 2 cut(s) 93, 304
BseLI CCNNNNNNNGG 2 cut(s) 174, 857
BseMII CTCAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 85
BshFI GGCC 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 521, 828
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 2 cut(s) 147, 513
BslI CCNNNNNNNGG 2 cut(s) 174, 857
BsmAI GTCTC 3 cut(s) 129, 383, 686
BsmBI CGTCTC 1 cut(s) 129
BsnI GGCC 1 cut(s) 652
Bso31I GGTCTC 1 cut(s) 686
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 521, 828
Bsp1407I TGTACA 1 cut(s) 496
Bsp143I GATC 1 cut(s) 808
Bsp1720I GCTNAGC 1 cut(s) 41
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 6 cut(s) 5, 11, 18, 175, 243, 649
BspANI GGCC 1 cut(s) 652
BspCNI CTCAG 1 cut(s) 33
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 2 cut(s) 170, 323
BspPI GGATC 1 cut(s) 816
BspTI CTTAAG 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 686
BsrGI TGTACA 1 cut(s) 496
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 1 cut(s) 808
BssNAI GTATAC 1 cut(s) 863
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 65
Bst1107I GTATAC 1 cut(s) 863
Bst2UI CCWGG 1 cut(s) 961
Bst4CI ACNGT 2 cut(s) 501, 746
Bst6I CTCTTC 4 cut(s) 16, 132, 405, 676
BstACI GRCGYC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 404
BstAUI TGTACA 1 cut(s) 496
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 93, 304
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 1 cut(s) 811
BstMAI GTCTC 3 cut(s) 129, 383, 686
BstMBI GATC 1 cut(s) 808
BstMWI GCNNNNNNNGC 5 cut(s) 70, 99, 415, 725, 767
BstNI CCWGG 1 cut(s) 961
BstSCI CCNGG 2 cut(s) 512, 959
BstSFI CTRYAG 1 cut(s) 774
BstV1I GCAGC 1 cut(s) 85
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
BstZ17I GTATAC 1 cut(s) 863
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 1 cut(s) 652
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 2 cut(s) 93, 304
BtsIMutI CAGTG 3 cut(s) 233, 807, 944
BveI ACCTGC 2 cut(s) 170, 323
Cac8I GCNNGC 1 cut(s) 358
CfoI GCGC 1 cut(s) 188
Cfr13I GGNCC 3 cut(s) 395, 651, 742
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 497
CviAII CATG 2 cut(s) 66, 365
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 810
DpnII GATC 1 cut(s) 808
Eam1104I CTCTTC 4 cut(s) 16, 132, 405, 676
EarI CTCTTC 4 cut(s) 16, 132, 405, 676
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 2 cut(s) 519, 826
Eco130I CCWWGG 1 cut(s) 65
Eco24I GRGCYC 2 cut(s) 521, 828
Eco31I GGTCTC 1 cut(s) 686
Eco47I GGWCC 2 cut(s) 395, 742
Eco53kI GAGCTC 2 cut(s) 519, 826
Eco88I CYCGRG 1 cut(s) 731
EcoICRI GAGCTC 2 cut(s) 519, 826
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 959
EcoT14I CCWWGG 1 cut(s) 65
EcoT38I GRGCYC 2 cut(s) 521, 828
ErhI CCWWGG 1 cut(s) 65
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 2 cut(s) 69, 368
FaiI YATR 7 cut(s) 67, 222, 366, 626, 656, 675, 863
FatI CATG 2 cut(s) 65, 364
FauI CCCGC 1 cut(s) 168
FblI GTMKAC 1 cut(s) 862
Fnu4HI GCNGC 2 cut(s) 74, 650
FokI GGATG 2 cut(s) 80, 311
FriOI GRGCYC 2 cut(s) 521, 828
Fsp4HI GCNGC 2 cut(s) 74, 650
FspBI CTAG 1 cut(s) 410
GlaI GCGC 1 cut(s) 187
GluI GCNGC 2 cut(s) 74, 650
GsuI CTGGAG 1 cut(s) 804
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 1 cut(s) 652
HapII CCGG 2 cut(s) 147, 513
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 188
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 2 cut(s) 69, 368
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 2 cut(s) 509, 797
HpaII CCGG 2 cut(s) 147, 513
HphI GGTGA 4 cut(s) 151, 314, 350, 983
Hpy166II GTNNAC 2 cut(s) 527, 863
Hpy188I TCNGA 3 cut(s) 286, 796, 955
Hpy188III TCNNGA 2 cut(s) 467, 821
Hpy8I GTNNAC 2 cut(s) 527, 863
HpyAV CCTTC 4 cut(s) 46, 129, 267, 344
HpyCH4III ACNGT 2 cut(s) 501, 746
HpyCH4IV ACGT 1 cut(s) 909
HpyCH4V TGCA 5 cut(s) 215, 665, 728, 776, 920
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 99, 415, 725, 767
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 1 cut(s) 909
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 2 cut(s) 69, 368
HspAI GCGC 1 cut(s) 186
Kzo9I GATC 1 cut(s) 808
LmnI GCTCC 3 cut(s) 70, 516, 823
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 1 cut(s) 102
MaeI CTAG 1 cut(s) 410
MaeII ACGT 1 cut(s) 909
MaeIII GTNAC 1 cut(s) 746
MalI GATC 1 cut(s) 810
MboI GATC 1 cut(s) 808
MboII GAAGA 9 cut(s) 33, 61, 119, 122, 263, 359, 392, 693, 890
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 2 cut(s) 521, 828
MluCI AATT 4 cut(s) 256, 620, 666, 698
MmeI TCCRAC 1 cut(s) 720
MnlI CCTC 9 cut(s) 108, 164, 218, 280, 298, 408, 677, 863, 912
MseI TTAA 2 cut(s) 405, 414
MspCI CTTAAG 1 cut(s) 404
MspI CCGG 2 cut(s) 147, 513
MspR9I CCNGG 2 cut(s) 514, 961
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 961
MwoI GCNNNNNNNGC 5 cut(s) 70, 99, 415, 725, 767
NciI CCSGG 1 cut(s) 514
NcoI CCATGG 1 cut(s) 65
NdeII GATC 1 cut(s) 808
NlaIII CATG 2 cut(s) 69, 368
PaqCI CACCTGC 2 cut(s) 170, 323
PfeI GAWTC 2 cut(s) 509, 797
PfoI TCCNGGA 1 cut(s) 512
PkrI GCNGC 2 cut(s) 75, 651
PpuMI RGGWCCY 1 cut(s) 395
Psp124BI GAGCTC 2 cut(s) 521, 828
Psp1406I AACGTT 1 cut(s) 909
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 959
PspGI CCWGG 1 cut(s) 959
PspPI GGNCC 3 cut(s) 395, 651, 742
PspPPI RGGWCCY 1 cut(s) 395
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 1 cut(s) 808
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
SacI GAGCTC 2 cut(s) 521, 828
SaqAI TTAA 2 cut(s) 405, 414
SatI GCNGC 2 cut(s) 74, 650
Sau3AI GATC 1 cut(s) 808
Sau96I GGNCC 3 cut(s) 395, 651, 742
ScrFI CCNGG 2 cut(s) 514, 961
SduI GDGCHC 2 cut(s) 521, 828
SfaNI GCATC 1 cut(s) 102
SfcI CTRYAG 1 cut(s) 774
SinI GGWCC 2 cut(s) 395, 742
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 4 cut(s) 256, 620, 666, 698
SsiI CCGC 6 cut(s) 5, 11, 18, 175, 243, 649
SspMI CTAG 1 cut(s) 410
SstI GAGCTC 2 cut(s) 521, 828
StyD4I CCNGG 2 cut(s) 512, 959
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 2 cut(s) 501, 746
TaiI ACGT 1 cut(s) 912
TaqI TCGA 1 cut(s) 105
TaqII GACCGA 1 cut(s) 710
TasI AATT 4 cut(s) 256, 620, 666, 698
TatI WGTACW 1 cut(s) 496
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 2 cut(s) 509, 797
Tru1I TTAA 2 cut(s) 405, 414
Tru9I TTAA 2 cut(s) 405, 414
TscAI CASTG 3 cut(s) 233, 807, 951
TseI GCWGC 1 cut(s) 73
TspDTI ATGAA 3 cut(s) 122, 594, 641
TspRI CASTG 3 cut(s) 233, 807, 951
Vha464I CTTAAG 1 cut(s) 404
VpaK11BI GGWCC 2 cut(s) 395, 742
XapI RAATTY 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 862
XspI CTAG 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.