Rorug04G0363200

Protein transport protein Sec24-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000554
Physical Location & Seq
Forward (+)
53567916 .. 53570357
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0363200.1

Sequence Viewer

Length: 960 bp
ATGCCTGAATATTGTGTGACTGGAGGTACAGGGTTCATAGCTGCTTACTTGGTGAAGGCTTTGCTAGAAAATGGCCATGTTGTGCGGGCCACTGTGCGAGACCCAGAGGATTTGGGGAAGGTCGGTTATCTGCGGGAGTTGAATGAAGCCAAGGAAAGGCTTAAGCTGATCAAAGCTGATCTAATGGTGGAAGGAAGCTTTGATGAAGCCATACAAGGCGTGGATGGGGTTTTCCACACCGCATCACCAGTGCTCGTTCCGTACGATAACAATGTTCAGGCGACTTTGATTGATCCATGTATAAAGGGCACCTTGAATGTGCTGAGTTCCTGTTCGAAGGCACGCGTGAAAAGGGTTGTGCTCACATCTTCTTGTTCTTCAATAAGATACCGTTACGATGCCCAACAGGCCTCTCCTCTCAATGACTCACATTGGAGCGATCCTGAATACTGCAAACGCTTCAATCTTTGGTATGCCTATGCAAAGACAACAGCGGAAAAAGAGGCGTGGAGAATCGCAGAGGAGAGTGGAATGGATCTAGTGGTGGTGAATCCGTCTTTCGTGGTTGGTCCTCTGCTAGCACCACAACCTTCAAGCACAATACTGATGATACTGGCAATACTTAAAGGTTTAAGAGGGGAATACCCAAATACAACAGTAGGATTTGTGCACATAGATGATGTTGTAGCTACACACATTCTGGCAATGGAGGAAAGAAAAGCATCAGGCAGGCTTATATGTTCAAGTTCGGTAGCGCACTGGTCGCAGATCATTGAGATGCTCAAGGCCAAATATCCATCTTATTCATATGAAAGCAAGTGTAGCAGCAAGGAAGGAGACAATAACCCACATAGCATGGACACCACTAAAATTGCTCAACTGGGTTTTCCTCCTTTCAAAACGCTCGAAAAAATGTTTGATGACTGCATCACAAGTTTTCAAGAGAAAGGGTTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

319

Amino Acids

35.36

Weight (kDa)

6.01

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 4 - 128 9.5e-06 KR domain
Epimerase PF01370 5 - 241 3.3e-29 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 5 - 124 1.2e-07 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 6 - 237 5.5e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 6 - 265 2.7e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 6 - 198 1.7e-14 Male sterility protein
NmrA PF05368 6 - 87 9.1e-10 NmrA-like family
NAD_binding_10 PF13460 8 - 187 6.1e-16 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000554)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT4G32640 AT4G32640
fragaria_vesca FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000
malus_domestica MD02G1169200.v1.1 MD13G1230500.v1.1 MD15G1281700.v1.1 MD16G1009900.v1.1 MD16G1235300.v1.1
prunus_persica Prupe.1G056500_v2.0.a1 Prupe.1G056500_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1
pyrus_communis pycom02g13590 pycom13g20290 pycom15g24480 pycom16g00970 pycom16g19630
rosa_chinensis RchiOBHm_Chr4g0396591 RchiOBHm_Chr4g0445631
rosa_laevigata RLG00000005723 RLG00000009518
rosa_multiflora Rmu_co8031254.1_g000001 Rmu_co8351119.1_g000001 Rmu_sc0003637.1_g000024 Rmu_sc0010969.1_g000001 Rmu_sc0019383.1_g000002
rosa_roxburghii Rroxscaffold_5G00341440 Rroxscaffold_5G00386110
rosa_rugosa Rorug03G0348600 Rorug03G0348700 Rorug04G0363200 Rorug04G0363300 Rorug04G0363400 Rorug04G0363500
rosa_samantha Rh4AG070600 Rh4AG424700 Rh4AG425100 Rh4BG068500 Rh4BG436900 Rh4CG076300 Rh4CG451400 Rh4DG065600 Rh4DG433400
rosa_wichuraiana Rw4G005630 Rw4G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 1 cut(s) 345
AciI CCGC 4 cut(s) 85, 133, 240, 494
AclWI GGATC 3 cut(s) 287, 434, 543
AcoI YGGCCR 1 cut(s) 73
AfaI GTAC 2 cut(s) 28, 263
AfiI CCNNNNNNNGG 1 cut(s) 156
AflII CTTAAG 1 cut(s) 161
AflIII ACRYGT 1 cut(s) 343
AgsI TTSAA 8 cut(s) 142, 316, 381, 463, 594, 744, 898, 941
AluBI AGCT 5 cut(s) 41, 166, 176, 198, 689
AluI AGCT 5 cut(s) 41, 166, 176, 198, 689
Alw21I GWGCWC 3 cut(s) 255, 363, 672
Alw26I GTCTC 2 cut(s) 93, 831
Alw44I GTGCAC 1 cut(s) 668
AlwI GGATC 3 cut(s) 287, 434, 543
AoxI GGCC 4 cut(s) 73, 87, 408, 786
ApaLI GTGCAC 1 cut(s) 668
ApeKI GCWGC 2 cut(s) 41, 825
AspLEI GCGC 1 cut(s) 757
AspS9I GGNCC 2 cut(s) 87, 569
AsuHPI GGTGA 3 cut(s) 64, 237, 559
AsuII TTCGAA 1 cut(s) 335
AsuNHI GCTAGC 1 cut(s) 577
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 2 cut(s) 311, 672
BalI TGGCCA 1 cut(s) 75
BanI GGYRCC 1 cut(s) 308
Bbv12I GWGCWC 3 cut(s) 255, 363, 672
BbvI GCAGC 2 cut(s) 28, 837
BccI CCATC 2 cut(s) 218, 805
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 93, 831
BfaI CTAG 3 cut(s) 65, 539, 578
BfrI CTTAAG 1 cut(s) 161
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 2 cut(s) 42, 826
BlsI GCNGC 2 cut(s) 43, 827
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 2 cut(s) 87, 569
BmiI GGNNCC 1 cut(s) 310
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 5 cut(s) 251, 388, 731, 768, 936
BmtI GCTAGC 1 cut(s) 581
BmuI ACTGGG 1 cut(s) 890
BpmI CTGGAG 1 cut(s) 42
Bpu14I TTCGAA 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 767
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 5 cut(s) 25, 248, 618, 764, 885
Bse3DI GCAATG 1 cut(s) 711
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 5 cut(s) 25, 248, 618, 764, 885
BseRI GAGGAG 2 cut(s) 405, 536
BseSI GKGCMC 2 cut(s) 311, 672
BseXI GCAGC 2 cut(s) 28, 837
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 4 cut(s) 75, 89, 410, 788
BshNI GGYRCC 1 cut(s) 308
BsiHKAI GWGCWC 3 cut(s) 255, 363, 672
BsiWI CGTACG 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 93, 831
BsnI GGCC 4 cut(s) 75, 89, 410, 788
Bso31I GGTCTC 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 335
Bsp1286I GDGCHC 4 cut(s) 255, 311, 363, 672
Bsp143I GATC 6 cut(s) 168, 178, 292, 439, 535, 768
BspACI CCGC 4 cut(s) 85, 133, 240, 494
BspANI GGCC 4 cut(s) 75, 89, 410, 788
BspCNI CTCAG 1 cut(s) 315
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 310
BspOI GCTAGC 1 cut(s) 581
BspPI GGATC 3 cut(s) 287, 434, 543
BspT104I TTCGAA 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 308
BspTI CTTAAG 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 5 cut(s) 25, 248, 618, 764, 885
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 6 cut(s) 168, 178, 292, 439, 535, 768
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 94, 392, 658
BstAFI CTTAAG 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 4 cut(s) 87, 343, 579, 731
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 1 cut(s) 345
BstHHI GCGC 1 cut(s) 757
BstKTI GATC 6 cut(s) 171, 181, 295, 442, 538, 771
BstMAI GTCTC 2 cut(s) 93, 831
BstMBI GATC 6 cut(s) 168, 178, 292, 439, 535, 768
BstMWI GCNNNNNNNGC 3 cut(s) 407, 763, 822
BstSLI GKGCMC 2 cut(s) 311, 672
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 28, 837
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BsuRI GGCC 4 cut(s) 75, 89, 410, 788
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 3 cut(s) 90, 255, 757
Cac8I GCNNGC 4 cut(s) 87, 343, 579, 731
CfoI GCGC 1 cut(s) 757
Cfr13I GGNCC 2 cut(s) 87, 569
Csp6I GTAC 2 cut(s) 27, 262
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 3 cut(s) 77, 297, 856
CviQI GTAC 2 cut(s) 27, 262
DdeI CTNAG 1 cut(s) 323
DpnI GATC 6 cut(s) 170, 180, 294, 441, 537, 770
DpnII GATC 6 cut(s) 168, 178, 292, 439, 535, 768
EaeI YGGCCR 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 569
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 80, 300, 859
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FatI CATG 3 cut(s) 76, 296, 855
FauI CCCGC 2 cut(s) 78, 126
FauNDI CATATG 1 cut(s) 808
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 2 cut(s) 42, 826
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 42, 826
FspBI CTAG 3 cut(s) 65, 539, 578
GlaI GCGC 1 cut(s) 756
GluI GCNGC 2 cut(s) 42, 826
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 4 cut(s) 75, 89, 410, 788
HhaI GCGC 1 cut(s) 757
Hin1II CATG 3 cut(s) 80, 300, 859
Hin6I GCGC 1 cut(s) 755
HinP1I GCGC 1 cut(s) 755
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 3 cut(s) 425, 513, 550
HphI GGTGA 3 cut(s) 64, 237, 559
Hpy166II GTNNAC 1 cut(s) 670
Hpy188III TCNNGA 2 cut(s) 443, 941
Hpy8I GTNNAC 1 cut(s) 670
HpyAV CCTTC 6 cut(s) 49, 112, 185, 331, 600, 827
HpyCH4III ACNGT 3 cut(s) 94, 392, 658
HpyCH4V TGCA 4 cut(s) 453, 482, 670, 927
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 763, 822
HpyF3I CTNAG 1 cut(s) 323
Hsp92II CATG 3 cut(s) 80, 300, 859
HspAI GCGC 1 cut(s) 755
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 6 cut(s) 168, 178, 292, 439, 535, 768
LmnI GCTCC 1 cut(s) 435
Lsp1109I GCAGC 2 cut(s) 28, 837
LweI GCATC 5 cut(s) 251, 388, 731, 768, 936
MaeI CTAG 3 cut(s) 65, 539, 578
MaeIII GTNAC 2 cut(s) 16, 392
MalI GATC 6 cut(s) 170, 180, 294, 441, 537, 770
MboI GATC 6 cut(s) 168, 178, 292, 439, 535, 768
MboII GAAGA 2 cut(s) 360, 369
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 4 cut(s) 255, 311, 363, 672
MlsI TGGCCA 1 cut(s) 75
MluCI AATT 1 cut(s) 870
MluI ACGCGT 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 75
MlyI GAGTC 1 cut(s) 419
Mox20I TGGCCA 1 cut(s) 75
MscI TGGCCA 1 cut(s) 75
MseI TTAA 3 cut(s) 162, 624, 632
MslI CAYNNNNRTG 2 cut(s) 675, 776
Msp20I TGGCCA 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 494
MspCI CTTAAG 1 cut(s) 161
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 407, 763, 822
NdeI CATATG 1 cut(s) 808
NdeII GATC 6 cut(s) 168, 178, 292, 439, 535, 768
NheI GCTAGC 1 cut(s) 577
NlaIII CATG 3 cut(s) 80, 300, 859
NlaIV GGNNCC 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 16
NspV TTCGAA 1 cut(s) 335
PceI AGGCCT 1 cut(s) 410
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PfeI GAWTC 2 cut(s) 513, 550
Pfl23II CGTACG 1 cut(s) 261
PkrI GCNGC 2 cut(s) 43, 827
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PspLI CGTACG 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 310
PspPI GGNCC 2 cut(s) 87, 569
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 2 cut(s) 28, 263
RsaNI GTAC 2 cut(s) 27, 262
RseI CAYNNNNRTG 2 cut(s) 675, 776
SaqAI TTAA 3 cut(s) 162, 624, 632
SatI GCNGC 2 cut(s) 42, 826
Sau3AI GATC 6 cut(s) 168, 178, 292, 439, 535, 768
Sau96I GGNCC 2 cut(s) 87, 569
SchI GAGTC 1 cut(s) 419
SduI GDGCHC 4 cut(s) 255, 311, 363, 672
SfaNI GCATC 5 cut(s) 251, 388, 731, 768, 936
SfuI TTCGAA 1 cut(s) 335
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 2 cut(s) 675, 776
SmlI CTYRAG 2 cut(s) 161, 782
SmoI CTYRAG 2 cut(s) 161, 782
Sse9I AATT 1 cut(s) 870
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 4 cut(s) 85, 133, 240, 494
SspI AATATT 1 cut(s) 11
SspMI CTAG 3 cut(s) 65, 539, 578
StuI AGGCCT 1 cut(s) 410
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 3 cut(s) 94, 392, 658
TaqI TCGA 2 cut(s) 335, 906
TasI AATT 1 cut(s) 870
TfiI GAWTC 2 cut(s) 513, 550
Tru1I TTAA 3 cut(s) 162, 624, 632
Tru9I TTAA 3 cut(s) 162, 624, 632
TscAI CASTG 3 cut(s) 97, 255, 764
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 2 cut(s) 41, 825
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 5 cut(s) 25, 159, 219, 795, 825
TspGWI ACGGA 2 cut(s) 249, 543
TspRI CASTG 3 cut(s) 97, 255, 764
Vha464I CTTAAG 1 cut(s) 161
VneI GTGCAC 1 cut(s) 668
VpaK11BI GGWCC 1 cut(s) 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XspI CTAG 3 cut(s) 65, 539, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.