MD13G1230500.v1.1

Protein transport protein Sec24-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000554
Physical Location & Seq
Forward (+)
22627593 .. 22641653
14061 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1230500.v1.1.491

Sequence Viewer

Length: 3192 bp
ATGCAGCCCCGCCCTGATCCATTAGCCGATAATATGCAGAATTTGAATCTTAATCGGCCGCCTTCCATGCCTAATTCTGCCCCTCCTAGACCATCCCCTTTCGGCCAACCGCCGCCTTTTCCTTCATCAGCCCCTCCTGCTGCTGCACCACCTCCTTTTTCACGGCCTGGCCCTCCCCCGGCTGCATTTGCAAGACCTACAGCACCAGCACCGCGATCGGGGGCCCCGCAACCAACATTATCCCCTGCCACCACCCCCGTAAAACCCTCCGGGCAGCCCGTTGGCCAGCCTTCATCTTTTCCGTCTCGACCGCCTCCTGTGGGTGGGGTAGCTACTGCGTCTGCGCCATCGCCAATTGGTCCAGTTTCGACGCCAGTGCCGGCATATCCTCGGCCAGGTCCTCAGAGCATGCCGCCGACGACTGCACCAGGTAGAATGATGAGTAATGGGCCGCCAATGTTTGGTTCCGGGGCTATGCCGGGTGGGCCTCGTTTCCCTCCGAGTGGAAATGCATCTCAACCACCTGTTGGGCATCCACCAGCAATGGCATGGGCACCACCAATGGGTCCTCCACGAACTCCAACCATGCACTCTGTGCTGGGTGATCCAGCAGTTAGTGGCCCACCAGGTACTACTGTTCAGCAGCCACCACCATTTTCAGCAGCACCGCCATTTCCAGCAGCACCTCAACCAATGCGGCCACCACCTCCAGGTTCTCCCTATGCCTCACAGACGTGGCAAGTGCAACAAGGACAGGTGGCTCCACCTTCACATTTTCCGGGTTCTGCTCAACCACCTAGGATGTTTGGAATGCCACCCCCACCGTTATCAAATCAGTCCATGACTACTATCTCACCTGCTGGTCAAACTGGTACTCCTTTGGCTGGGTCATCCAAGATTGATCCCACTCAAATTCCTCGGCCTATACCAAGTTCATCGGTGCTCATTCATGAAACTCGTCAGGGCAATCAGGCAAATCCACCTCCGCCTGCTACAACTGATTATATTGTCAGAGACACTGGGAATTGCAGTCCACGATACATGAGGTGCACCATCAATCAGATACCATGCACTGCTGATCTTTTAACAACATCAGGGATGCCCTTAGCTTTGTTGGTTGAACCTTTTGCCCTTCCTCATCCAAATGAGGAACCAATCCAAGTTGTGGATTTTGGTGAAAGTGGTCCCGTTCGATGCTCTCGTTGCAAAGGCTACATAAATCCTTTTATGAAGTTCATTGACCAGGGCAGGAAATTCATCTGTAATCTTTGTGGATTTACCGATGAGACTCCCCGTGACTACCACTGCAATCTTGGTCCAGATGGTAGACGTAGAGATGCTGATGAGAGGCCTGAGCTATGTAGAGGAACAGTTGAATTTGTTGCTTCAAAAGAATACATGGTCCGTGATCCCATGCCAGCTGTGTACTTCTTCCTCATTGATGTATCCATGAATGCCATTCAAACTGGTGCAACTGCTGCAGCTTGCAGTGCAATCAGTCAAGTTATTGCTGATCTTCCAGAAGGTCCTCGGACAATGGTGGGAATTGCAACATTTGATTCAACGATCCATTTTTACAATTTGAAACGTGCATTGCAGCAGCCTTTGATGCTTATTGTTCCTGATGTGCAAGATGTTTACACTCCTCTGGAGACTGACGTAGTTGTCCAGCTTTCTGAGTGCCACCAACATTTGGAGCAATTGTTGGAAAGTATTCCAACCATGTTTCAAAACAGTAAAATCGCTGAATCAGCCTTTGGTGCAGCAATCAAGGCTGCTTTCTTGGCAATTAAGAGTACTGGAGGGAAACTTTTGGTGTTTCAGTCAGTGTTGGCATCAACTGGAATTGGAGCACTCTCTGCTAGAGAAGCTGAAGGAAGAGCTAATATATCTTCAGCTGATAAGGAGCCCCATAAATTACTCCAACCAGCTGACAAAACTTTAAGGACAATGGCAGTTGAATTTGCTGAATATCAGGTTTGTGTCGACTTATTTATCACTACCCAGTCATACATAGACATTGCTTCTGTCTCTGTCATCCCAAGAACAACTGGAGGGCAGGTTTACTATTATTATCCCTTCTCAGCTGTCTCTGATCCTGCAAAGCTCTACAACGATCTTAAATGGAATGTCACAAGGCCTCAAGGTTTTGAGGCGGTGATGCGTGTAAGATGCTGCCAGGGTATCCAAGTTCAAGAGTACCATGGAAGCTTTTGCAAACGTATTCCTACGGATGTTGACCTACCTGGGATTGACTGTGACAAAACTATTATGGTGACCTTAAAACATGATGATAAATTACAGGATGGCTCAGAATGTGGTTTCCAGTGTGCCGTTTTATACACAACTGTGTATGGCCAACGGAGAATTCGAGTTGCAACTTTATCTCTGCCATGCACAAGTATGCTAAGTAATCTGTTCCGTGCTGCTGATTTGGACGCTCAATTTGCATGTTTCATGAAGCAAGCGGCAATCGAAATCCCTTCAAGTCCCCTCTTGCGTGTACGGGAACAAGTGACAAATCTGTGCATAAGTAGTCTGCTTTCATATCGTAAATTTTGTGCTACTGTATCATCTTCTGGACAGCTTATTCTTCCGGAGGCACTGAAGCTCCTGCCTCTGTACACCCTTGCATTAATCAAAAGTACGGGGCTACGGACTGATGGAAAAATAGATGAGCGGTCCTTCTGGATCAATCATGTTTCTTCCCTTTCTGTTCCTTTGGCAGTTCCATTGGTGTACCCCAGAATGGTGGCTATTCATGACTTCGAATCAAAAAAGGAGGGTGATGAGTCTCTAATTCCTCCGGTGATTCCGCTTTCTAGCGAACATGTGAGTGACGAGGGAATTTATCTTCTCGAGAATGGCGAGGACTGTTTAATATATATTGGGAACTTGGTAGATTCTAGAATTTTGCAACAACTGTTTGGAATCGCCTCTGCTGATGAACTTCCTACTCAGTTTGTGCTACAGTGGTATGATAATCCATTATCGAAGAAGCTGAATGATGTGGTAAATGAAATACGGCGCCAGAGATGTTCCTACCTTCGCTTAAAGTTATGCAAGAAAGGAGACCCATCAGGAACATTGTTTTTCTCATATATGGTCGAAGACCAGAGTCCGAATGGCCCCACCTATGTTGAGTTTCTGGTACACGTCCATCGGCAAATACAGATAAAAATGGTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

1064

Amino Acids

115.04

Weight (kDa)

7.19

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 396 - 434 1.5e-16 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 471 - 711 4.5e-79 Sec23/Sec24 trunk domain
Sec23_BS PF08033 716 - 800 6e-25 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 811 - 909 1.8e-24 Sec23/Sec24 helical domain
Gelsolin PF00626 935 - 1001 1.6e-06 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000554)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT4G32640 AT4G32640
fragaria_vesca FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000
malus_domestica MD02G1169200.v1.1 MD13G1230500.v1.1 MD15G1281700.v1.1 MD16G1009900.v1.1 MD16G1235300.v1.1
prunus_persica Prupe.1G056500_v2.0.a1 Prupe.1G056500_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1
pyrus_communis pycom02g13590 pycom13g20290 pycom15g24480 pycom16g00970 pycom16g19630
rosa_chinensis RchiOBHm_Chr4g0396591 RchiOBHm_Chr4g0445631
rosa_laevigata RLG00000005723 RLG00000009518
rosa_multiflora Rmu_co8031254.1_g000001 Rmu_co8351119.1_g000001 Rmu_sc0003637.1_g000024 Rmu_sc0010969.1_g000001 Rmu_sc0019383.1_g000002
rosa_roxburghii Rroxscaffold_5G00341440 Rroxscaffold_5G00386110
rosa_rugosa Rorug03G0348600 Rorug03G0348700 Rorug04G0363200 Rorug04G0363300 Rorug04G0363400 Rorug04G0363500
rosa_samantha Rh4AG070600 Rh4AG424700 Rh4AG425100 Rh4BG068500 Rh4BG436900 Rh4CG076300 Rh4CG451400 Rh4DG065600 Rh4DG433400
rosa_wichuraiana Rw4G005630 Rw4G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 865
AasI GACNNNNNNGTC 1 cut(s) 1664
Acc36I ACCTGC 2 cut(s) 865, 2050
AccB1I GGYRCC 2 cut(s) 553, 3027
AccB7I CCANNNNNTGG 4 cut(s) 461, 527, 1165, 1693
AccBSI CCGCTC 1 cut(s) 2680
AccI GTMKAC 2 cut(s) 1327, 1986
AccII CGCG 1 cut(s) 214
AccIII TCCGGA 1 cut(s) 2596
AclWI GGATC 7 cut(s) 11, 599, 896, 1403, 1561, 2090, 2699
AcoI YGGCCR 6 cut(s) 56, 103, 283, 392, 698, 2356
AcsI RAATTY 9 cut(s) 40, 912, 1253, 1376, 1961, 2367, 2555, 2847, 2910
AcuI CTGAAG 3 cut(s) 1878, 1893, 2627
AcyI GRCGYC 2 cut(s) 371, 3028
AdeI CACNNNGTG 1 cut(s) 595
AflIII ACRYGT 2 cut(s) 2830, 3154
AjiI CACGTC 2 cut(s) 735, 3157
AjnI CCWGG 8 cut(s) 166, 394, 427, 625, 709, 1242, 2178, 2245
AjuI GAANNNNNNNTTGG 6 cut(s) 448, 480, 1740, 1772, 2352, 2384
AleI CACNNNNGTG 2 cut(s) 733, 2348
Alw21I GWGCWC 3 cut(s) 945, 1052, 1855
Alw26I GTCTC 8 cut(s) 309, 1008, 1280, 1646, 2035, 2095, 2799, 3066
Alw44I GTGCAC 1 cut(s) 1048
AlwI GGATC 7 cut(s) 11, 599, 896, 1403, 1561, 2090, 2699
Ama87I CYCGRG 1 cut(s) 2858
Aor13HI TCCGGA 1 cut(s) 2596
ApaI GGGCCC 1 cut(s) 226
ApaLI GTGCAC 1 cut(s) 1048
ApoI RAATTY 9 cut(s) 40, 912, 1253, 1376, 1961, 2367, 2555, 2847, 2910
ArsI GACNNNNNNTTYG 2 cut(s) 2429, 2461
AseI ATTAAT 1 cut(s) 2636
Asp700I GAANNNNTTC 1 cut(s) 1713
AspA2I CCTAGG 1 cut(s) 797
AspLEI GCGC 2 cut(s) 346, 3030
AsuC2I CCSGG 5 cut(s) 179, 271, 469, 480, 780
AsuHPI GGTGA 7 cut(s) 614, 846, 1187, 2170, 2287, 2798, 2821
AsuII TTCGAA 1 cut(s) 2769
AvaI CYCGRG 1 cut(s) 2858
AvaII GGWCC 8 cut(s) 359, 398, 566, 1184, 1316, 1402, 1526, 2682
AvrII CCTAGG 1 cut(s) 797
BaeGI GKGCMC 3 cut(s) 226, 556, 1052
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BalI TGGCCA 2 cut(s) 285, 2358
BanI GGYRCC 2 cut(s) 553, 3027
BanII GRGCYC 2 cut(s) 226, 1911
BbsI GAAGAC 1 cut(s) 3117
Bbv12I GWGCWC 3 cut(s) 945, 1052, 1855
BccI CCATC 8 cut(s) 100, 355, 1061, 1316, 2300, 2657, 3085, 3168
BceAI ACGGC 3 cut(s) 179, 2318, 3041
BcgI CGANNNNNNTGC 4 cut(s) 198, 232, 358, 392
BciT130I CCWGG 8 cut(s) 168, 396, 429, 627, 711, 1244, 2180, 2247
BciVI GTATCC 2 cut(s) 1456, 2195
BcnI CCSGG 5 cut(s) 179, 271, 469, 480, 780
BcoDI GTCTC 8 cut(s) 309, 1008, 1280, 1646, 2035, 2095, 2799, 3066
BfaI CTAG 5 cut(s) 87, 798, 1863, 2823, 2907
BfmI CTRYAG 3 cut(s) 198, 1479, 2969
BfoI RGCGCY 1 cut(s) 3031
BfuAI ACCTGC 2 cut(s) 865, 2050
BfuI GTATCC 2 cut(s) 1456, 2195
BglI GCCNNNNNGGC 1 cut(s) 484
BlnI CCTAGG 1 cut(s) 797
BmcAI AGTACT 1 cut(s) 1798
Bme18I GGWCC 8 cut(s) 359, 398, 566, 1184, 1316, 1402, 1526, 2682
BmeT110I CYCGRG 1 cut(s) 2858
BmgBI CACGTC 2 cut(s) 735, 3157
BmrI ACTGGG 2 cut(s) 1029, 1999
BmsI GCATC 9 cut(s) 521, 541, 1089, 1184, 1327, 1599, 1844, 2151, 2162
BmuI ACTGGG 2 cut(s) 1029, 1999
BoxI GACNNNNGTC 1 cut(s) 3117
BpiI GAAGAC 1 cut(s) 3117
BpmI CTGGAG 4 cut(s) 693, 1670, 1821, 2073
Bpu10I CCTNAGC 2 cut(s) 1105, 1353
Bpu14I TTCGAA 1 cut(s) 2769
BpuEI CTTGAG 1 cut(s) 2127
BpuMI CCSGG 5 cut(s) 179, 271, 469, 480, 780
BsaHI GRCGYC 2 cut(s) 371, 3028
BsaI GGTCTC 1 cut(s) 3066
BsaWI WCCGGW 2 cut(s) 2596, 2806
BsaXI ACNNNNNCTCC 8 cut(s) 859, 889, 1688, 1718, 2046, 2076, 2595, 2625
Bse118I RCCGGY 1 cut(s) 379
Bse3DI GCAATG 3 cut(s) 549, 1592, 2019
BseAI TCCGGA 1 cut(s) 2596
BseBI CCWGG 8 cut(s) 168, 396, 429, 627, 711, 1244, 2180, 2247
BseGI GGATG 9 cut(s) 92, 532, 807, 890, 1104, 1138, 2037, 2239, 2311
BseMI GCAATG 3 cut(s) 549, 1592, 2019
BseMII CTCAG 6 cut(s) 416, 1344, 1668, 2097, 2325, 2972
BseRI GAGGAG 1 cut(s) 1635
BseSI GKGCMC 3 cut(s) 226, 556, 1052
BseX3I CGGCCG 1 cut(s) 56
BseYI CCCAGC 2 cut(s) 598, 884
BsgI GTGCAG 3 cut(s) 129, 408, 1782
Bsh1236I CGCG 1 cut(s) 214
Bsh1285I CGRYCG 3 cut(s) 59, 218, 311
BshNI GGYRCC 2 cut(s) 553, 3027
BsiEI CGRYCG 3 cut(s) 59, 218, 311
BsiHKAI GWGCWC 3 cut(s) 945, 1052, 1855
BsiHKCI CYCGRG 1 cut(s) 2858
BsiSI CCGG 8 cut(s) 179, 270, 380, 468, 479, 779, 2597, 2807
BslFI GGGAC 2 cut(s) 1170, 2475
BsmAI GTCTC 8 cut(s) 309, 1008, 1280, 1646, 2035, 2095, 2799, 3066
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 2 cut(s) 1170, 2475
BsmI GAATGC 2 cut(s) 816, 1459
Bso31I GGTCTC 1 cut(s) 3066
BsoBI CYCGRG 1 cut(s) 2858
Bsp119I TTCGAA 1 cut(s) 2769
Bsp120I GGGCCC 1 cut(s) 222
Bsp1286I GDGCHC 6 cut(s) 226, 556, 945, 1052, 1855, 1911
Bsp13I TCCGGA 1 cut(s) 2596
Bsp1407I TGTACA 1 cut(s) 2622
Bsp19I CCATGG 1 cut(s) 2203
BspCNI CTCAG 6 cut(s) 415, 1345, 1669, 2096, 2324, 2971
BspEI TCCGGA 1 cut(s) 2596
BspFNI CGCG 1 cut(s) 214
BspHI TCATGA 3 cut(s) 949, 2457, 2761
BspMAI CTGCAG 1 cut(s) 1483
BspMI ACCTGC 2 cut(s) 865, 2050
BspPI GGATC 7 cut(s) 11, 599, 896, 1403, 1561, 2090, 2699
BspQI GCTCTTC 1 cut(s) 1873
BspT104I TTCGAA 1 cut(s) 2769
BspT107I GGYRCC 2 cut(s) 553, 3027
BspTNI GGTCTC 1 cut(s) 3066
BsrBI CCGCTC 1 cut(s) 2680
BsrDI GCAATG 3 cut(s) 549, 1592, 2019
BsrFI RCCGGY 1 cut(s) 379
BsrGI TGTACA 1 cut(s) 2622
BssAI RCCGGY 1 cut(s) 379
BssNI GRCGYC 2 cut(s) 371, 3028
BssT1I CCWWGG 2 cut(s) 797, 2203
Bst2UI CCWGG 8 cut(s) 168, 396, 429, 627, 711, 1244, 2180, 2247
Bst6I CTCTTC 1 cut(s) 1873
BstACI GRCGYC 2 cut(s) 371, 3028
BstAPI GCANNNNNTGC 3 cut(s) 595, 1478, 1859
BstAUI TGTACA 1 cut(s) 2622
BstBI TTCGAA 1 cut(s) 2769
BstC8I GCNNGC 7 cut(s) 287, 381, 410, 990, 1419, 1486, 2466
BstDEI CTNAG 8 cut(s) 402, 1105, 1353, 1677, 2083, 2311, 2408, 2958
BstDSI CCRYGG 1 cut(s) 2203
BstEII GGTNACC 1 cut(s) 2275
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 9 cut(s) 92, 532, 807, 890, 1104, 1138, 2037, 2239, 2311
BstFNI CGCG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 3031
BstHHI GCGC 2 cut(s) 346, 3030
BstMAI GTCTC 8 cut(s) 309, 1008, 1280, 1646, 2035, 2095, 2799, 3066
BstMCI CGRYCG 3 cut(s) 59, 218, 311
BstNI CCWGG 8 cut(s) 168, 396, 429, 627, 711, 1244, 2180, 2247
BstNSI RCATGY 3 cut(s) 412, 2454, 2834
BstPAI GACNNNNGTC 1 cut(s) 3117
BstPI GGTNACC 1 cut(s) 2275
BstSFI CTRYAG 3 cut(s) 198, 1479, 2969
BstSLI GKGCMC 3 cut(s) 226, 556, 1052
BstUI CGCG 1 cut(s) 214
BstV2I GAAGAC 1 cut(s) 3117
BstXI CCANNNNNNTGG 1 cut(s) 2752
BstZI CGGCCG 1 cut(s) 56
BsuI GTATCC 2 cut(s) 1456, 2195
BtgI CCRYGG 1 cut(s) 2203
BtgZI GCGATG 1 cut(s) 333
BtrI CACGTC 2 cut(s) 735, 3157
BtsCI GGATG 9 cut(s) 92, 532, 807, 890, 1104, 1138, 2037, 2239, 2311
BtsI GCAGTG 3 cut(s) 1071, 1303, 1495
BtsIMutI CAGTG 9 cut(s) 381, 1017, 1071, 1303, 1495, 1833, 2333, 2603, 2978
BveI ACCTGC 2 cut(s) 865, 2050
Cac8I GCNNGC 7 cut(s) 287, 381, 410, 990, 1419, 1486, 2466
CciI TCATGA 3 cut(s) 949, 2457, 2761
CfoI GCGC 2 cut(s) 346, 3030
Cfr10I RCCGGY 1 cut(s) 379
CseI GACGC 3 cut(s) 327, 379, 2447
CsiI ACCWGGT 2 cut(s) 427, 625
CspCI CAANNNNNGTGG 2 cut(s) 3121, 3156
DdeI CTNAG 8 cut(s) 402, 1105, 1353, 1677, 2083, 2311, 2408, 2958
DinI GGCGCC 1 cut(s) 3029
DraIII CACNNNGTG 1 cut(s) 595
DrdI GACNNNNNNGTC 1 cut(s) 1664
DseDI GACNNNNNNGTC 1 cut(s) 1664
EaeI YGGCCR 6 cut(s) 56, 103, 283, 392, 698, 2356
EagI CGGCCG 1 cut(s) 56
Eam1104I CTCTTC 1 cut(s) 1873
EarI CTCTTC 1 cut(s) 1873
EciI GGCGGA 1 cut(s) 975
EclXI CGGCCG 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 797, 2203
Eco147I AGGCCT 2 cut(s) 1351, 2140
Eco24I GRGCYC 2 cut(s) 226, 1911
Eco31I GGTCTC 1 cut(s) 3066
Eco47I GGWCC 8 cut(s) 359, 398, 566, 1184, 1316, 1402, 1526, 2682
Eco52I CGGCCG 1 cut(s) 56
Eco57I CTGAAG 3 cut(s) 1878, 1893, 2627
Eco88I CYCGRG 1 cut(s) 2858
Eco91I GGTNACC 1 cut(s) 2275
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoO109I RGGNCCY 5 cut(s) 222, 223, 398, 566, 1526
EcoO65I GGTNACC 1 cut(s) 2275
EcoRI GAATTC 1 cut(s) 2367
EcoRII CCWGG 8 cut(s) 166, 394, 427, 625, 709, 1242, 2178, 2245
EcoT14I CCWWGG 2 cut(s) 797, 2203
EcoT22I ATGCAT 1 cut(s) 514
EcoT38I GRGCYC 2 cut(s) 226, 1911
EgeI GGCGCC 1 cut(s) 3029
EheI GGCGCC 1 cut(s) 3029
ErhI CCWWGG 2 cut(s) 797, 2203
Esp3I CGTCTC 1 cut(s) 309
FaqI GGGAC 2 cut(s) 1170, 2475
FauI CCCGC 2 cut(s) 17, 234
FblI GTMKAC 2 cut(s) 1327, 1986
FokI GGATG 9 cut(s) 79, 519, 814, 877, 1111, 1125, 2024, 2246, 2318
FriOI GRGCYC 2 cut(s) 226, 1911
FspBI CTAG 5 cut(s) 87, 798, 1863, 2823, 2907
GlaI GCGC 2 cut(s) 345, 3029
GsaI CCCAGC 2 cut(s) 602, 888
GsuI CTGGAG 4 cut(s) 693, 1670, 1821, 2073
HaeII RGCGCY 1 cut(s) 3031
HapII CCGG 8 cut(s) 179, 270, 380, 468, 479, 779, 2597, 2807
HgaI GACGC 3 cut(s) 327, 379, 2447
HhaI GCGC 2 cut(s) 346, 3030
Hin1I GRCGYC 2 cut(s) 371, 3028
Hin6I GCGC 2 cut(s) 344, 3028
HinP1I GCGC 2 cut(s) 344, 3028
HincII GTYRAC 2 cut(s) 1987, 2239
HindII GTYRAC 2 cut(s) 1987, 2239
HindIII AAGCTT 1 cut(s) 2209
HpaII CCGG 8 cut(s) 179, 270, 380, 468, 479, 779, 2597, 2807
HphI GGTGA 7 cut(s) 614, 846, 1187, 2170, 2287, 2798, 2821
Hpy188I TCNGA 9 cut(s) 405, 501, 1013, 1062, 1533, 1678, 2095, 2314, 3123
Hpy99I CGWCG 2 cut(s) 373, 421
HpyCH4IV ACGT 6 cut(s) 734, 1330, 1588, 1659, 2221, 3156
HpyF3I CTNAG 8 cut(s) 402, 1105, 1353, 1677, 2083, 2311, 2408, 2958
HpySE526I ACGT 6 cut(s) 734, 1330, 1588, 1659, 2221, 3156
Hsp92I GRCGYC 2 cut(s) 371, 3028
HspAI GCGC 2 cut(s) 344, 3028
KasI GGCGCC 1 cut(s) 3027
Kpn2I TCCGGA 1 cut(s) 2596
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
LguI GCTCTTC 1 cut(s) 1873
LmnI GCTCC 5 cut(s) 766, 1696, 1850, 1906, 2616
LweI GCATC 9 cut(s) 521, 541, 1089, 1184, 1327, 1599, 1844, 2151, 2162
MabI ACCWGGT 2 cut(s) 427, 625
MaeI CTAG 5 cut(s) 87, 798, 1863, 2823, 2907
MaeII ACGT 6 cut(s) 734, 1330, 1588, 1659, 2221, 3156
MaeIII GTNAC 6 cut(s) 1295, 2131, 2258, 2275, 2515, 2837
MbiI CCGCTC 1 cut(s) 2680
MfeI CAATTG 2 cut(s) 354, 1700
MhlI GDGCHC 6 cut(s) 226, 556, 945, 1052, 1855, 1911
MlsI TGGCCA 2 cut(s) 285, 2358
MluNI TGGCCA 2 cut(s) 285, 2358
Mly113I GGCGCC 1 cut(s) 3028
MlyI GAGTC 3 cut(s) 1282, 2801, 3127
MmeI TCCRAC 4 cut(s) 605, 1686, 1742, 1948
Mox20I TGGCCA 2 cut(s) 285, 2358
Mph1103I ATGCAT 1 cut(s) 514
MroI TCCGGA 1 cut(s) 2596
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 1713
MscI TGGCCA 2 cut(s) 285, 2358
MseI TTAA 9 cut(s) 51, 1085, 1791, 1943, 2121, 2282, 2636, 2879, 3053
MslI CAYNNNNRTG 5 cut(s) 733, 1143, 2348, 2402, 2835
Msp20I TGGCCA 2 cut(s) 285, 2358
MspA1I CMGCKG 4 cut(s) 1421, 1898, 1931, 2087
MspI CCGG 8 cut(s) 179, 270, 380, 468, 479, 779, 2597, 2807
MunI CAATTG 2 cut(s) 354, 1700
Mva1269I GAATGC 2 cut(s) 816, 1459
MvaI CCWGG 8 cut(s) 168, 396, 429, 627, 711, 1244, 2180, 2247
MvnI CGCG 1 cut(s) 214
NaeI GCCGGC 1 cut(s) 381
NarI GGCGCC 1 cut(s) 3028
NciI CCSGG 5 cut(s) 179, 271, 469, 480, 780
NcoI CCATGG 1 cut(s) 2203
NgoMIV GCCGGC 1 cut(s) 379
NmeAIII GCCGAG 2 cut(s) 370, 898
NmuCI GTSAC 6 cut(s) 1295, 2131, 2258, 2275, 2515, 2837
NsiI ATGCAT 1 cut(s) 514
NspI RCATGY 3 cut(s) 412, 2454, 2834
NspV TTCGAA 1 cut(s) 2769
OliI CACNNNNGTG 2 cut(s) 733, 2348
PaeI GCATGC 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 2858
PagI TCATGA 3 cut(s) 949, 2457, 2761
PaqCI CACCTGC 1 cut(s) 865
PceI AGGCCT 2 cut(s) 1351, 2140
PciI ACATGT 1 cut(s) 2830
PciSI GCTCTTC 1 cut(s) 1873
PcsI WCGNNNNNNNCGW 3 cut(s) 1198, 2368, 2865
PctI GAATGC 2 cut(s) 816, 1459
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 1713
PfeI GAWTC 7 cut(s) 46, 1559, 1748, 2771, 2812, 2903, 2931
PflMI CCANNNNNTGG 4 cut(s) 461, 527, 1165, 1693
Ple19I CGATCG 1 cut(s) 218
PleI GAGTC 3 cut(s) 1282, 2800, 3126
PluTI GGCGCC 1 cut(s) 3031
PpsI GAGTC 3 cut(s) 1282, 2800, 3126
PpuMI RGGWCCY 3 cut(s) 398, 566, 1526
PscI ACATGT 1 cut(s) 2830
PshAI GACNNNNGTC 1 cut(s) 3117
PshBI ATTAAT 1 cut(s) 2636
Psp5II RGGWCCY 3 cut(s) 398, 566, 1526
Psp6I CCWGG 8 cut(s) 166, 394, 427, 625, 709, 1242, 2178, 2245
PspEI GGTNACC 1 cut(s) 2275
PspFI CCCAGC 2 cut(s) 598, 884
PspGI CCWGG 8 cut(s) 166, 394, 427, 625, 709, 1242, 2178, 2245
PspOMI GGGCCC 1 cut(s) 222
PspPPI RGGWCCY 3 cut(s) 398, 566, 1526
PstI CTGCAG 1 cut(s) 1483
PvuI CGATCG 1 cut(s) 218
PvuII CAGCTG 4 cut(s) 1421, 1898, 1931, 2087
RseI CAYNNNNRTG 5 cut(s) 733, 1143, 2348, 2402, 2835
SalI GTCGAC 1 cut(s) 1985
SapI GCTCTTC 1 cut(s) 1873
SaqAI TTAA 9 cut(s) 51, 1085, 1791, 1943, 2121, 2282, 2636, 2879, 3053
ScaI AGTACT 1 cut(s) 1798
SchI GAGTC 3 cut(s) 1282, 2801, 3127
SduI GDGCHC 6 cut(s) 226, 556, 945, 1052, 1855, 1911
SexAI ACCWGGT 2 cut(s) 427, 625
SfaNI GCATC 9 cut(s) 521, 541, 1089, 1184, 1327, 1599, 1844, 2151, 2162
SfcI CTRYAG 3 cut(s) 198, 1479, 2969
SfoI GGCGCC 1 cut(s) 3029
Sfr274I CTCGAG 1 cut(s) 2858
SfuI TTCGAA 1 cut(s) 2769
SinI GGWCC 8 cut(s) 359, 398, 566, 1184, 1316, 1402, 1526, 2682
SlaI CTCGAG 1 cut(s) 2858
SmiMI CAYNNNNRTG 5 cut(s) 733, 1143, 2348, 2402, 2835
SmlI CTYRAG 2 cut(s) 2142, 2858
SmoI CTYRAG 2 cut(s) 2142, 2858
SphI GCATGC 1 cut(s) 412
SseBI AGGCCT 2 cut(s) 1351, 2140
SspDI GGCGCC 1 cut(s) 3027
SspMI CTAG 5 cut(s) 87, 798, 1863, 2823, 2907
StuI AGGCCT 2 cut(s) 1351, 2140
StyI CCWWGG 2 cut(s) 797, 2203
TaiI ACGT 6 cut(s) 737, 1333, 1591, 1662, 2224, 3159
TatI WGTACW 3 cut(s) 1425, 1796, 2622
TauI GCSGC 6 cut(s) 61, 115, 415, 454, 700, 2471
TfiI GAWTC 7 cut(s) 46, 1559, 1748, 2771, 2812, 2903, 2931
Tru1I TTAA 9 cut(s) 51, 1085, 1791, 1943, 2121, 2282, 2636, 2879, 3053
Tru9I TTAA 9 cut(s) 51, 1085, 1791, 1943, 2121, 2282, 2636, 2879, 3053
TscAI CASTG 9 cut(s) 381, 1024, 1078, 1310, 1495, 1833, 2333, 2610, 2978
TseFI GTSAC 6 cut(s) 1295, 2131, 2258, 2275, 2515, 2837
Tsp45I GTSAC 6 cut(s) 1295, 2131, 2258, 2275, 2515, 2837
TspGWI ACGGA 6 cut(s) 291, 1394, 2246, 2377, 2411, 2671
TspRI CASTG 9 cut(s) 381, 1024, 1078, 1310, 1495, 1833, 2333, 2610, 2978
Van91I CCANNNNNTGG 4 cut(s) 461, 527, 1165, 1693
VneI GTGCAC 1 cut(s) 1048
VpaK11BI GGWCC 8 cut(s) 359, 398, 566, 1184, 1316, 1402, 1526, 2682
VspI ATTAAT 1 cut(s) 2636
XagI CCTNNNNNAGG 1 cut(s) 393
XapI RAATTY 9 cut(s) 40, 912, 1253, 1376, 1961, 2367, 2555, 2847, 2910
XbaI TCTAGA 1 cut(s) 2906
XceI RCATGY 3 cut(s) 412, 2454, 2834
XcmI CCANNNNNNNNNTGG 3 cut(s) 546, 1310, 3122
XhoI CTCGAG 1 cut(s) 2858
XmaJI CCTAGG 1 cut(s) 797
XmiI GTMKAC 2 cut(s) 1327, 1986
XmnI GAANNNNTTC 1 cut(s) 1713
XspI CTAG 5 cut(s) 87, 798, 1863, 2823, 2907
ZrmI AGTACT 1 cut(s) 1798
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.