Rw4G036410

Protein transport protein Sec24-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000554
Physical Location & Seq
Reverse (-)
63216174 .. 63225146
8973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036410.1

Sequence Viewer

Length: 3132 bp
ATGGTGACTCCGGTGCCTCCAGGGCCACCTAGACCTGGTAATCATCCACCAAACCCTAATGTTAACCCCAATACCCAATCTTTATCTGATAATATGCAAAATTTGAATCTCAATCGGCCGCCACCGCCTTCAATGCCCCATCCTAGACCTTCATCGACGCCGTATCCGGCGCCATTTTCTAGGCCTGGCCCTCCTACTCCGGCGCCGGTGCGGCCTTCTGGACCCCCACCGCCTTCATTTCCTCCGGGAAGGCCTTCTGGCCCGCCTGTTAGTCAAAGTCAACCTTCTCCACTTGGCTACAGACCACCTCCTCCTACCGGTACTTTCCCACCGAACCGTCAAATGGCACCACCTCGGCCACCGCCTGGTGCCCGACTGGGCGGGATGGGGCCTCCTCCTCCAGTTATGCCTTCACCAGGTGCTGTGATGAATAATGGGCAGCCGAATTTTGCACCAGGAGGTTTGCAGGCTGGGCCACGTTTTCCTCCTCCAGCTGGGATTGCATCACAACTGACGCCGCCGCCTCCTCTCGGACCTCCAACAGCAATGGCAGGAGCTTGGGCGCCACCGCAGCCTCAAACCATGCATGCTTTTTTGGGCAGTGCTAGTCCTCCAGTGCAACAGCAGCGATCACCATTTGCAGGGGCAGCCCAACCTTCTTCAGTTCCTCCTTATGGATCACAACCTTGGTCAATGCAACCCAACCAAGTGCCTCCACCTCCACCAATTTCCCAATCTCCGCAAGCACCTAGGATGTTTGGAATGCCACCACCCATGCCAAATCAGTCTATGACTGCCATATCACATGCTGTGGGTCAAACTGGAGCCCCAGTGGCAGGTCCATCTAAGATTGATCCTAGTCAAATTCCGCGACCCGTTCCAGATTCCTCCGTTGTTCTGCATGAAACTCGCCAAGGCAATCAGGCCAACCCTCCTCCGCCTGCTACAAGCGATTACATTGTCAGAGACACTGGAAACTGCAGCCCACGTAATATGAGGTGCACTATCAATCAGATCCCATGCACTGCTGACCTCTTGACGACATCAGGGATGCAGTTGGCTTTGCTGGTCCAACCCTTGGCCCTTCCTCATCCTTCTGAAGAGCCCATACAAGTCGTGGATTTAGGTGAAAGTGGTCCTGTTCGTTGTTCTCGTTGCAAAGGCTATATAAATCCTTTCATGAAGTTCATTGATCAGGGAAGGCAGTTCATCTGCAACTTGTGTGGATTTACTGATGAGACTCCCCGTGACTACCACTGCAACCTTGGTCCAGATGGTAGGCGTAGAGATGCTGATGATAGACCCGAGCTTTGTAGAGGAATGGTGGAAATTGTTGCTCCAAAAGAATACATGGTGCGTGATCCAATGCTGGCTATGTATTTCTTCCTTATTGATGTATCCATGAATGCCATACAAACTGGAGCAACTGCAGCTGCATGCACTGCAATCAGTCAAGTTATTTCCGATCTTCCTGAAGGTCCTCGGACAAAGGTGGGAATTGCAACATTTGACTGTACTATTCATTTTTATAATTTGAAACGTGCTTTACAGCAGCCATTGATGCTCATTGTTCCTGATATACAAGATGTTTATACTCCTCTGGAGACTGATGTGATTGTTCAGCTTTCTGAGTGCCGTCAGCATTTAGAGCTACTGCTTGAAAGCATCCCATCCATGTTTGAAAATAACAAAACAGCTGACTCAGCCTTTGGTGCTGCAGTCAAGGCTGCATTCTTGGCAATGAAAAGTAATGGAGGAAAACTTTTGGTATTTCAATCAGTATTGCCATCTGTTGGGACTGGAGCCCTTTCCGCTAGAGAGGCTGAAGGAAGAACGAATACTTCTGCTGGTGAGAAGGAGGCCCATAAATTACTTCAACCAGCCGATAAGACTTTAGAATCAATGGCAATGGAAGTTGCTGAGTATCAGGTTTGTGTAGATATTTTTATTACCACCCAGACTTACATGGACATAGCTTCTATCTCTGTCATCCCAAGAACTACTGGTGGACAGATCTATTATTATTGCCCTTTCTCTGCTGTTACTGACACTGCAAAGATCTACAATGATCTTAAATGGAATATTACAAGGCCTCAAGGTTTTGAGGCAGTGATGCGTGTGCGATGCAGCCAGGGTCTCCAAGTTAACGAGTACCGCGGGAACTTCTGCAAGCGCATTCCTACAGATGTTGACCTACCTGGGATCGACTCTGACAAAACTATAATGGTAACCATAAAACATGATGACAAATTGCAGGACGGCTCAGAATGTGTGTTTCAGTGTGCGCTTCTGTACACTACTGTGTATGGCCAAAGAATGATTCGAGTTTTAACACTAGCCCTACCATGCACAAGTATGCTGAATAATCTTTTCCGCACAGCTGATTTGGATACTCAGTTTGCTTGTATCTTGAAGCAAGGGGCTAATGAAATCCCTTCTAGCCCACTCCTGCAAGTCCGTGAACGACTGACTGACCGTTGCATCAGCATTCTCTGTTCTTATCGTAAATTTTGTGCTACAGTAACATCAAGTGGACAACTTATTCTTCCAGAAACACTGAAGCTTCTACCTCTATACATCCTTGCTTTAACGAAAAGTACAGGATTGCGATCTTCTGGGAAAATAGATGAACGATCTTTTTGGATCAACTATGTGTCCTCTGTTTCCACTGCTTTAGCAGTAGCACTTGTTTATCCACGGATGCTGGCAGTCCACAACCTTAATTGTAAGGATGACGCATCTCTTTGCCCTCCTGCAATTCCACTTTCTAGTGAGCATATCAGTGATGAGGGAATTTATCTTCTCGAAAATGGAGAGGATTGTCTAATTTATGTAGGGAGCTCAGTTGATCCAGATATCTTGAACAAGTTGTTTGGAATTCCCTCAGTTGATGAAATTCCTACTCAGTTTGTCCTGCAGCAGTATGACAATCCATTCTCAAAGAAACTAAATGATTTGATAAATGAGATACGACGCCAAAGATGTTCCTACCTCCGCTTGAAGTTATGCAAGAAAGGAGATCCATCAGGGATGCTTTTCTTCTCTTATATCGTAGAAGACAAGAGCTCGATTGGCCTCTCATATGTTGAGTTCCTAATACATATCCATCGGCAAATTCAAATGAAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

1043

Amino Acids

113.46

Weight (kDa)

7.66

Isoelectric Point (pI)

53.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 380 - 418 2.5e-16 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 455 - 695 5.3e-77 Sec23/Sec24 trunk domain
Sec23_BS PF08033 700 - 784 4.6e-26 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 795 - 892 6.5e-25 Sec23/Sec24 helical domain
Gelsolin PF00626 917 - 985 2.3e-07 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000554)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT4G32640 AT4G32640
fragaria_vesca FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000
malus_domestica MD02G1169200.v1.1 MD13G1230500.v1.1 MD15G1281700.v1.1 MD16G1009900.v1.1 MD16G1235300.v1.1
prunus_persica Prupe.1G056500_v2.0.a1 Prupe.1G056500_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1
pyrus_communis pycom02g13590 pycom13g20290 pycom15g24480 pycom16g00970 pycom16g19630
rosa_chinensis RchiOBHm_Chr4g0396591 RchiOBHm_Chr4g0445631
rosa_laevigata RLG00000005723 RLG00000009518
rosa_multiflora Rmu_co8031254.1_g000001 Rmu_co8351119.1_g000001 Rmu_sc0003637.1_g000024 Rmu_sc0010969.1_g000001 Rmu_sc0019383.1_g000002
rosa_roxburghii Rroxscaffold_5G00341440 Rroxscaffold_5G00386110
rosa_rugosa Rorug03G0348600 Rorug03G0348700 Rorug04G0363200 Rorug04G0363300 Rorug04G0363400 Rorug04G0363500
rosa_samantha Rh4AG070600 Rh4AG424700 Rh4AG425100 Rh4BG068500 Rh4BG436900 Rh4CG076300 Rh4CG451400 Rh4DG065600 Rh4DG433400
rosa_wichuraiana Rw4G005630 Rw4G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1530
Acc36I ACCTGC 1 cut(s) 827
AccB1I GGYRCC 6 cut(s) 13, 169, 202, 346, 368, 562
AccB7I CCANNNNNTGG 3 cut(s) 365, 1078, 1793
AccII CGCG 2 cut(s) 871, 2157
AclWI GGATC 8 cut(s) 685, 848, 1009, 1355, 2210, 2651, 2843, 3014
AcoI YGGCCR 3 cut(s) 116, 356, 2308
AcsI RAATTY 8 cut(s) 100, 445, 864, 2507, 2793, 2877, 2895, 3114
AcuI CTGAAG 5 cut(s) 645, 1119, 1494, 1845, 2579
AcyI GRCGYC 6 cut(s) 158, 170, 203, 515, 563, 2974
AdeI CACNNNGTG 1 cut(s) 419
AfaI GTAC 5 cut(s) 322, 1516, 2153, 2294, 2599
AgeI ACCGGT 1 cut(s) 317
AjnI CCWGG 8 cut(s) 19, 34, 184, 364, 415, 454, 2130, 2197
AjuI GAANNNNNNNTTGG 2 cut(s) 2304, 2336
AleI CACNNNNGTG 1 cut(s) 2300
Alw21I GWGCWC 3 cut(s) 1004, 2843, 3068
Alw26I GTCTC 4 cut(s) 960, 1232, 1598, 2141
Alw44I GTGCAC 1 cut(s) 1000
AlwI GGATC 8 cut(s) 685, 848, 1009, 1355, 2210, 2651, 2843, 3014
AlwNI CAGNNNCTG 1 cut(s) 422
Ama87I CYCGRG 1 cut(s) 1304
ApaLI GTGCAC 1 cut(s) 1000
ApoI RAATTY 8 cut(s) 100, 445, 864, 2507, 2793, 2877, 2895, 3114
ArsI GACNNNNNNTTYG 4 cut(s) 1293, 1325, 1691, 1723
AsiGI ACCGGT 1 cut(s) 317
Asp700I GAANNNNTTC 1 cut(s) 253
AspA2I CCTAGG 1 cut(s) 749
AspLEI GCGC 5 cut(s) 172, 205, 565, 2175, 2287
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 5 cut(s) 16, 405, 624, 1139, 1862
AvaI CYCGRG 1 cut(s) 1304
AvaII GGWCC 7 cut(s) 221, 533, 839, 1069, 1136, 1268, 1478
AvrII CCTAGG 1 cut(s) 749
BaeGI GKGCMC 2 cut(s) 373, 1004
BalI TGGCCA 1 cut(s) 2310
BanI GGYRCC 6 cut(s) 13, 169, 202, 346, 368, 562
BanII GRGCYC 5 cut(s) 829, 1107, 1807, 2843, 3068
BarI GAAGNNNNNNTAC 4 cut(s) 1092, 1124, 1822, 1854
BbsI GAAGAC 1 cut(s) 3063
Bbv12I GWGCWC 3 cut(s) 1004, 2843, 3068
BccI CCATC 8 cut(s) 147, 379, 850, 1268, 1678, 1795, 3031, 3114
BceAI ACGGC 3 cut(s) 145, 1620, 2275
BcgI CGANNNNNNTGC 2 cut(s) 890, 924
BciT130I CCWGG 8 cut(s) 21, 36, 186, 366, 417, 456, 2132, 2199
BciVI GTATCC 3 cut(s) 174, 1408, 2383
BclI TGATCA 1 cut(s) 1192
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 4 cut(s) 960, 1232, 1598, 2141
BfmI CTRYAG 7 cut(s) 298, 979, 1428, 1716, 2181, 2517, 2915
BfoI RGCGCY 3 cut(s) 173, 206, 566
BfuAI ACCTGC 1 cut(s) 827
BfuI GTATCC 3 cut(s) 174, 1408, 2383
BglI GCCNNNNNGGC 3 cut(s) 22, 211, 833
BglII AGATCT 2 cut(s) 2013, 2058
BlnI CCTAGG 1 cut(s) 749
Bme1390I CCNGG 9 cut(s) 21, 36, 186, 246, 366, 417, 456, 2132, 2199
Bme18I GGWCC 7 cut(s) 221, 533, 839, 1069, 1136, 1268, 1478
BmeT110I CYCGRG 1 cut(s) 1304
BmrFI CCNGG 9 cut(s) 21, 36, 186, 246, 366, 417, 456, 2132, 2199
BmrI ACTGGG 2 cut(s) 386, 824
BmuI ACTGGG 2 cut(s) 386, 824
BpiI GAAGAC 1 cut(s) 3063
BpmI CTGGAG 7 cut(s) 384, 474, 597, 843, 1440, 1622, 1821
BpuEI CTTGAG 1 cut(s) 2079
BpuMI CCSGG 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 989
BsaBI GATNNNNATC 1 cut(s) 2608
BsaHI GRCGYC 6 cut(s) 158, 170, 203, 515, 563, 2974
BsaI GGTCTC 1 cut(s) 2141
BsaWI WCCGGW 2 cut(s) 10, 317
BsaXI ACNNNNNCTCC 2 cut(s) 816, 846
Bse118I RCCGGY 2 cut(s) 205, 317
Bse1I ACTGG 9 cut(s) 381, 401, 614, 826, 830, 976, 1423, 1804, 2008
Bse3DI GCAATG 3 cut(s) 552, 1746, 1914
Bse8I GATNNNNATC 1 cut(s) 2608
BseBI CCWGG 8 cut(s) 21, 36, 186, 366, 417, 456, 2132, 2199
BseJI GATNNNNATC 1 cut(s) 2608
BseMI GCAATG 3 cut(s) 552, 1746, 1914
BseMII CTCAG 8 cut(s) 1620, 1716, 1911, 2277, 2408, 2856, 2898, 2918
BseNI ACTGG 9 cut(s) 381, 401, 614, 826, 830, 976, 1423, 1804, 2008
BseRI GAGGAG 7 cut(s) 300, 384, 387, 477, 516, 924, 1587
BseSI GKGCMC 2 cut(s) 373, 1004
BseX3I CGGCCG 1 cut(s) 116
BseYI CCCAGC 2 cut(s) 470, 494
Bsh1236I CGCG 2 cut(s) 871, 2157
Bsh1285I CGRYCG 1 cut(s) 119
BshNI GGYRCC 6 cut(s) 13, 169, 202, 346, 368, 562
BshTI ACCGGT 1 cut(s) 317
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 3 cut(s) 1004, 2843, 3068
BsiHKCI CYCGRG 1 cut(s) 1304
BsiSI CCGG 6 cut(s) 11, 167, 200, 206, 245, 318
BslFI GGGAC 1 cut(s) 1810
BsmAI GTCTC 4 cut(s) 960, 1232, 1598, 2141
BsmFI GGGAC 1 cut(s) 1810
BsmI GAATGC 5 cut(s) 768, 1411, 1730, 2175, 2487
Bso31I GGTCTC 1 cut(s) 2141
BsoBI CYCGRG 1 cut(s) 1304
Bsp1286I GDGCHC 7 cut(s) 373, 829, 1004, 1107, 1807, 2843, 3068
Bsp1407I TGTACA 1 cut(s) 2292
BspCNI CTCAG 8 cut(s) 1621, 1715, 1912, 2276, 2407, 2855, 2897, 2917
BspFNI CGCG 2 cut(s) 871, 2157
BspHI TCATGA 1 cut(s) 1179
BspMAI CTGCAG 4 cut(s) 983, 1432, 1720, 2919
BspMI ACCTGC 1 cut(s) 827
BspPI GGATC 8 cut(s) 685, 848, 1009, 1355, 2210, 2651, 2843, 3014
BspQI GCTCTTC 1 cut(s) 1095
BspT107I GGYRCC 6 cut(s) 13, 169, 202, 346, 368, 562
BspTNI GGTCTC 1 cut(s) 2141
BsrDI GCAATG 3 cut(s) 552, 1746, 1914
BsrFI RCCGGY 2 cut(s) 205, 317
BsrGI TGTACA 1 cut(s) 2292
BsrI ACTGG 9 cut(s) 381, 401, 614, 826, 830, 976, 1423, 1804, 2008
BssAI RCCGGY 2 cut(s) 205, 317
BssNI GRCGYC 6 cut(s) 158, 170, 203, 515, 563, 2974
BssT1I CCWWGG 5 cut(s) 686, 749, 913, 1077, 1264
Bst2UI CCWGG 8 cut(s) 21, 36, 186, 366, 417, 456, 2132, 2199
Bst4CI ACNGT 5 cut(s) 338, 1514, 2302, 2477, 2521
Bst6I CTCTTC 1 cut(s) 1095
BstACI GRCGYC 6 cut(s) 158, 170, 203, 515, 563, 2974
BstAPI GCANNNNNTGC 1 cut(s) 1442
BstAUI TGTACA 1 cut(s) 2292
BstBAI YACGTR 1 cut(s) 989
BstC8I GCNNGC 9 cut(s) 263, 468, 588, 744, 942, 1371, 1438, 2171, 2706
BstDEI CTNAG 9 cut(s) 846, 1629, 1702, 1920, 2263, 2394, 2842, 2884, 2904
BstDSI CCRYGG 2 cut(s) 2155, 2696
BstEII GGTNACC 1 cut(s) 2227
BstENI CCTNNNNNAGG 1 cut(s) 414
BstFNI CGCG 2 cut(s) 871, 2157
BstH2I RGCGCY 3 cut(s) 173, 206, 566
BstHHI GCGC 5 cut(s) 172, 205, 565, 2175, 2287
BstMAI GTCTC 4 cut(s) 960, 1232, 1598, 2141
BstMCI CGRYCG 1 cut(s) 119
BstNI CCWGG 8 cut(s) 21, 36, 186, 366, 417, 456, 2132, 2199
BstNSI RCATGY 3 cut(s) 590, 809, 1440
BstPI GGTNACC 1 cut(s) 2227
BstSCI CCNGG 9 cut(s) 19, 34, 184, 244, 364, 415, 454, 2130, 2197
BstSFI CTRYAG 7 cut(s) 298, 979, 1428, 1716, 2181, 2517, 2915
BstSLI GKGCMC 2 cut(s) 373, 1004
BstUI CGCG 2 cut(s) 871, 2157
BstV2I GAAGAC 1 cut(s) 3063
BstX2I RGATCY 4 cut(s) 1014, 2013, 2058, 3019
BstYI RGATCY 4 cut(s) 1014, 2013, 2058, 3019
BstZI CGGCCG 1 cut(s) 116
BsuI GTATCC 3 cut(s) 174, 1408, 2383
BtgI CCRYGG 2 cut(s) 2155, 2696
BtgZI GCGATG 1 cut(s) 2137
BtsI GCAGTG 7 cut(s) 607, 1023, 1255, 1440, 2049, 2115, 2667
BveI ACCTGC 1 cut(s) 827
Cac8I GCNNGC 9 cut(s) 263, 468, 588, 744, 942, 1371, 1438, 2171, 2706
CaiI CAGNNNCTG 1 cut(s) 422
CciI TCATGA 1 cut(s) 1179
CfoI GCGC 5 cut(s) 172, 205, 565, 2175, 2287
Cfr10I RCCGGY 2 cut(s) 205, 317
Cfr42I CCGCGG 1 cut(s) 2158
CseI GACGC 4 cut(s) 166, 523, 2744, 2982
CsiI ACCWGGT 2 cut(s) 34, 415
Csp6I GTAC 5 cut(s) 321, 1515, 2152, 2293, 2598
CspAI ACCGGT 1 cut(s) 317
CspCI CAANNNNNGTGG 2 cut(s) 1204, 1239
CviQI GTAC 5 cut(s) 321, 1515, 2152, 2293, 2598
DdeI CTNAG 9 cut(s) 846, 1629, 1702, 1920, 2263, 2394, 2842, 2884, 2904
DinI GGCGCC 3 cut(s) 171, 204, 564
DraIII CACNNNGTG 1 cut(s) 419
EaeI YGGCCR 3 cut(s) 116, 356, 2308
EagI CGGCCG 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 1095
EarI CTCTTC 1 cut(s) 1095
EciI GGCGGA 1 cut(s) 927
Ecl136II GAGCTC 2 cut(s) 2841, 3066
EclXI CGGCCG 1 cut(s) 116
Eco130I CCWWGG 5 cut(s) 686, 749, 913, 1077, 1264
Eco147I AGGCCT 3 cut(s) 184, 253, 2092
Eco24I GRGCYC 5 cut(s) 829, 1107, 1807, 2843, 3068
Eco31I GGTCTC 1 cut(s) 2141
Eco32I GATATC 1 cut(s) 2857
Eco47I GGWCC 7 cut(s) 221, 533, 839, 1069, 1136, 1268, 1478
Eco52I CGGCCG 1 cut(s) 116
Eco53kI GAGCTC 2 cut(s) 2841, 3066
Eco57I CTGAAG 5 cut(s) 645, 1119, 1494, 1845, 2579
Eco88I CYCGRG 1 cut(s) 1304
Eco91I GGTNACC 1 cut(s) 2227
EcoICRI GAGCTC 2 cut(s) 2841, 3066
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoO109I RGGNCCY 2 cut(s) 389, 1478
EcoO65I GGTNACC 1 cut(s) 2227
EcoRI GAATTC 1 cut(s) 2877
EcoRII CCWGG 8 cut(s) 19, 34, 184, 364, 415, 454, 2130, 2197
EcoRV GATATC 1 cut(s) 2857
EcoT14I CCWWGG 5 cut(s) 686, 749, 913, 1077, 1264
EcoT22I ATGCAT 1 cut(s) 588
EcoT38I GRGCYC 5 cut(s) 829, 1107, 1807, 2843, 3068
EgeI GGCGCC 3 cut(s) 171, 204, 564
EheI GGCGCC 3 cut(s) 171, 204, 564
ErhI CCWWGG 5 cut(s) 686, 749, 913, 1077, 1264
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FaqI GGGAC 1 cut(s) 1810
FauI CCCGC 3 cut(s) 270, 374, 2150
FauNDI CATATG 1 cut(s) 3082
FbaI TGATCA 1 cut(s) 1192
FriOI GRGCYC 5 cut(s) 829, 1107, 1807, 2843, 3068
GlaI GCGC 5 cut(s) 171, 204, 564, 2174, 2286
GsaI CCCAGC 2 cut(s) 474, 498
GsuI CTGGAG 7 cut(s) 384, 474, 597, 843, 1440, 1622, 1821
HaeII RGCGCY 3 cut(s) 173, 206, 566
HapII CCGG 6 cut(s) 11, 167, 200, 206, 245, 318
HgaI GACGC 4 cut(s) 166, 523, 2744, 2982
HhaI GCGC 5 cut(s) 172, 205, 565, 2175, 2287
Hin1I GRCGYC 6 cut(s) 158, 170, 203, 515, 563, 2974
Hin6I GCGC 5 cut(s) 170, 203, 563, 2173, 2285
HinP1I GCGC 5 cut(s) 170, 203, 563, 2173, 2285
HincII GTYRAC 4 cut(s) 64, 281, 2146, 2191
HindII GTYRAC 4 cut(s) 64, 281, 2146, 2191
HindIII AAGCTT 1 cut(s) 2561
HinfI GANTC 8 cut(s) 7, 106, 884, 1240, 1700, 1898, 2207, 2320
HpaI GTTAAC 2 cut(s) 64, 2146
HpaII CCGG 6 cut(s) 11, 167, 200, 206, 245, 318
HphI GGTGA 5 cut(s) 16, 405, 624, 1139, 1862
Hpy99I CGWCG 2 cut(s) 160, 2976
HpyCH4III ACNGT 5 cut(s) 338, 1514, 2302, 2477, 2521
HpyCH4IV ACGT 3 cut(s) 478, 988, 1540
HpyF3I CTNAG 9 cut(s) 846, 1629, 1702, 1920, 2263, 2394, 2842, 2884, 2904
HpySE526I ACGT 3 cut(s) 478, 988, 1540
Hsp92I GRCGYC 6 cut(s) 158, 170, 203, 515, 563, 2974
HspAI GCGC 5 cut(s) 170, 203, 563, 2173, 2285
KasI GGCGCC 3 cut(s) 169, 202, 562
Ksp22I TGATCA 1 cut(s) 1192
KspAI GTTAAC 2 cut(s) 64, 2146
KspI CCGCGG 1 cut(s) 2158
LguI GCTCTTC 1 cut(s) 1095
LmnI GCTCC 6 cut(s) 554, 824, 1342, 1421, 1802, 2838
MabI ACCWGGT 2 cut(s) 34, 415
MaeII ACGT 3 cut(s) 478, 988, 1540
MaeIII GTNAC 5 cut(s) 4, 1247, 2041, 2227, 2521
MflI RGATCY 4 cut(s) 1014, 2013, 2058, 3019
MhlI GDGCHC 7 cut(s) 373, 829, 1004, 1107, 1807, 2843, 3068
MlsI TGGCCA 1 cut(s) 2310
MluNI TGGCCA 1 cut(s) 2310
Mly113I GGCGCC 3 cut(s) 170, 203, 563
MlyI GAGTC 3 cut(s) 1234, 1694, 2201
MmeI TCCRAC 2 cut(s) 563, 1096
Mox20I TGGCCA 1 cut(s) 2310
Mph1103I ATGCAT 1 cut(s) 588
MroXI GAANNNNTTC 1 cut(s) 253
MscI TGGCCA 1 cut(s) 2310
MseI TTAA 6 cut(s) 63, 2073, 2145, 2330, 2588, 2721
MslI CAYNNNNRTG 3 cut(s) 2300, 2354, 2781
Msp20I TGGCCA 1 cut(s) 2310
MspA1I CMGCKG 5 cut(s) 494, 1433, 1697, 2157, 2381
MspI CCGG 6 cut(s) 11, 167, 200, 206, 245, 318
MspR9I CCNGG 9 cut(s) 21, 36, 186, 246, 366, 417, 456, 2132, 2199
Mva1269I GAATGC 5 cut(s) 768, 1411, 1730, 2175, 2487
MvaI CCWGG 8 cut(s) 21, 36, 186, 366, 417, 456, 2132, 2199
MvnI CGCG 2 cut(s) 871, 2157
NarI GGCGCC 3 cut(s) 170, 203, 563
NciI CCSGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 3082
NmeAIII GCCGAG 1 cut(s) 334
NmuCI GTSAC 2 cut(s) 4, 1247
NsiI ATGCAT 1 cut(s) 588
NspI RCATGY 3 cut(s) 590, 809, 1440
OliI CACNNNNGTG 1 cut(s) 2300
PaeI GCATGC 2 cut(s) 590, 1440
PagI TCATGA 1 cut(s) 1179
PceI AGGCCT 3 cut(s) 184, 253, 2092
PciSI GCTCTTC 1 cut(s) 1095
PcsI WCGNNNNNNNCGW 1 cut(s) 1150
PctI GAATGC 5 cut(s) 768, 1411, 1730, 2175, 2487
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 4 cut(s) 106, 884, 1898, 2320
PflMI CCANNNNNTGG 3 cut(s) 365, 1078, 1793
PfoI TCCNGGA 1 cut(s) 244
PinAI ACCGGT 1 cut(s) 317
PleI GAGTC 3 cut(s) 1234, 1694, 2201
PluTI GGCGCC 3 cut(s) 173, 206, 566
PpsI GAGTC 3 cut(s) 1234, 1694, 2201
Ppu21I YACGTR 1 cut(s) 989
PpuMI RGGWCCY 1 cut(s) 1478
PsiI TTATAA 1 cut(s) 1530
Psp124BI GAGCTC 2 cut(s) 2843, 3068
Psp5II RGGWCCY 1 cut(s) 1478
Psp6I CCWGG 8 cut(s) 19, 34, 184, 364, 415, 454, 2130, 2197
PspEI GGTNACC 1 cut(s) 2227
PspFI CCCAGC 2 cut(s) 470, 494
PspGI CCWGG 8 cut(s) 19, 34, 184, 364, 415, 454, 2130, 2197
PspPPI RGGWCCY 1 cut(s) 1478
PstI CTGCAG 4 cut(s) 983, 1432, 1720, 2919
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 4 cut(s) 1014, 2013, 2058, 3019
PvuII CAGCTG 4 cut(s) 494, 1433, 1697, 2381
RsaI GTAC 5 cut(s) 322, 1516, 2153, 2294, 2599
RsaNI GTAC 5 cut(s) 321, 1515, 2152, 2293, 2598
RseI CAYNNNNRTG 3 cut(s) 2300, 2354, 2781
SacI GAGCTC 2 cut(s) 2843, 3068
SacII CCGCGG 1 cut(s) 2158
SapI GCTCTTC 1 cut(s) 1095
SaqAI TTAA 6 cut(s) 63, 2073, 2145, 2330, 2588, 2721
SchI GAGTC 3 cut(s) 1234, 1694, 2201
ScrFI CCNGG 9 cut(s) 21, 36, 186, 246, 366, 417, 456, 2132, 2199
SduI GDGCHC 7 cut(s) 373, 829, 1004, 1107, 1807, 2843, 3068
SexAI ACCWGGT 2 cut(s) 34, 415
SfcI CTRYAG 7 cut(s) 298, 979, 1428, 1716, 2181, 2517, 2915
SfoI GGCGCC 3 cut(s) 171, 204, 564
Sfr303I CCGCGG 1 cut(s) 2158
SgrAI CRCCGGYG 1 cut(s) 205
SgrBI CCGCGG 1 cut(s) 2158
SinI GGWCC 7 cut(s) 221, 533, 839, 1069, 1136, 1268, 1478
SmiMI CAYNNNNRTG 3 cut(s) 2300, 2354, 2781
SmlI CTYRAG 1 cut(s) 2094
SmoI CTYRAG 1 cut(s) 2094
SphI GCATGC 2 cut(s) 590, 1440
SseBI AGGCCT 3 cut(s) 184, 253, 2092
SspDI GGCGCC 3 cut(s) 169, 202, 562
SspI AATATT 1 cut(s) 2083
SstI GAGCTC 2 cut(s) 2843, 3068
StuI AGGCCT 3 cut(s) 184, 253, 2092
StyD4I CCNGG 9 cut(s) 19, 34, 184, 244, 364, 415, 454, 2130, 2197
StyI CCWWGG 5 cut(s) 686, 749, 913, 1077, 1264
TaaI ACNGT 5 cut(s) 338, 1514, 2302, 2477, 2521
TaiI ACGT 3 cut(s) 481, 991, 1543
TaqI TCGA 5 cut(s) 155, 2205, 2323, 2805, 3068
TatI WGTACW 3 cut(s) 1514, 2292, 2597
TauI GCSGC 4 cut(s) 121, 214, 520, 523
TfiI GAWTC 4 cut(s) 106, 884, 1898, 2320
Tru1I TTAA 6 cut(s) 63, 2073, 2145, 2330, 2588, 2721
Tru9I TTAA 6 cut(s) 63, 2073, 2145, 2330, 2588, 2721
TseFI GTSAC 2 cut(s) 4, 1247
Tsp45I GTSAC 2 cut(s) 4, 1247
TspGWI ACGGA 3 cut(s) 880, 2447, 2713
Van91I CCANNNNNTGG 3 cut(s) 365, 1078, 1793
VneI GTGCAC 1 cut(s) 1000
VpaK11BI GGWCC 7 cut(s) 221, 533, 839, 1069, 1136, 1268, 1478
XagI CCTNNNNNAGG 1 cut(s) 414
XapI RAATTY 8 cut(s) 100, 445, 864, 2507, 2793, 2877, 2895, 3114
XceI RCATGY 3 cut(s) 590, 809, 1440
XcmI CCANNNNNNNNNTGG 2 cut(s) 1114, 1262
XmaJI CCTAGG 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 253
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.