MD16G1009900.v1.1

Protein transport protein Sec24-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000554
Physical Location & Seq
Forward (+)
769380 .. 776749
7370 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1009900.v1.1.491

Sequence Viewer

Length: 3027 bp
ATGGCAGCTCCAGGGCCACCCCGACCCGGTAATCGTCCACCGCCCCCTTATTACAACCCTAACCCAAATCGTATAATTCCTCCCCCAAATTCCGATTCTTTGTCGGACAATTTGCACAATTTGAATCTCAATCGACCGCCTTCGATGCCCACCTCTGCCCCAAGACCTTCACCTCATTCCCAACCACCGCCGTCAGCACCGCCGGGCCCATTTTCTCGGACTGCGCAGTCCAGACCTTTACCGGGTCCCCCTCCAGTGGGACCCCCCTCCTCGTTTCCACCGTACGTGGGCCCGTCAATGTCTTCCGGGCCTCCTGTAGCTTCACCACTTGGCAACAGACCGCCTCCTCCCAATGCTTTCCCGCCGGCACCCCCACCAGGTGTTTTTCTCGGCGGGCCCCGTTTTCCTCCACCTGTTTGCGGGACTGGACCTTCGGCAACAATGGCTGCAGCTCGGGCACCGCCTCCTCATCATCAGCCTCAGCCTCCGCCAACCATGCATTCTGTATTGGGCAGTTCAGTGGGCAGAGTGCCTCCAGGTCCGCCCGTGCAGCCGCCGCAACCAGTTTCTCAACCTCCACAAACGTGGTCCATGCATAATCAAGCACCGCAGCTGCCGCCAACTTCAGCGTCTGTGCAACCACCAAGAATGTTTGGCATGCCACCACCGTTGCCAAACCAATCCATGACTGCCATATCACATGCTGTGGGGCAAACTGGAGCTCCGGCGGCAGGACCGTCAAAGATTGATCCGAATCAAATACCACGACCTATTCCAAGTTCCTCAGTGGTTGTGTATGAAACTCGTCAGGGCAATCAAACAAACCCTCCTCCGCCTTCTACAAGTGATTACACTGTCAGAGACACTGGGAACTGCAGTCCACGCAATATGAGGTGCACCATTAATCAGGTTCCATGCACTGCTGACCTTTTGGCGACATCGGGCATGCAGTTGGCTTTGCTGCTCCAACCTTTGGCCCTTCCTCATCCATCCGAAGAGCCTATTCAAGTTGTGGATTTTGGTGAAAGTGGTCCTGTTCGTTGTTCTCGTTGCAAAGGCTACATAAACCCTTCCATGAAGTTCATTGATCAGGGCAGGAAGTTCATCTGTAGCTTGTGTGGATTTAGTGATGAGACGCCCCGTGATTACCACTGCAATCTTGGTCCAGATGGTAGGCGTAGAGATGCTGACGATAGGCCCGAGCTTTGTAAAGGAATGGTTGAATTTGTTGCTTCAAAAGAGTACATGGTGCGTGATCCAATGCTGGCTGTGTATTTCTTCCTTATTGATGTATCTATGATTGCTATGCAAACTGGAGCAACTGCGGCGGCATGCAGTGCAATCAGTCAAGTTATTTCTGATCTTCCTGAAGGCCCTAGGACTATGGTGGGAATTGCAACATTTGACACTACAATTCATTTTTACAATTTGAAACGTTCATTACAGCAGCCACTGATGCTCATTGTTCCTGATGTACAAGATGTTTATACTCCTCTGCAGACTGATGTAATTGTACAGATTTCTGAGTGCCGTCAACATTTAGAACTACTTTTGGAAAGCATCCCATCCATGTTTCAAGATAATAAAACAGCTGACTCAGCTTTTGGTGCCGCAGTCAAGGCTGCATTCTTGGCAATGAAAAGGAATGGAGGAAAACTTTTGGTATTTCAGTCAGTCTTGCCATCAATTGGAATTGGAGCCCTTTCAGCTAGAGAGGCTGAAGGAAGAACTAATAGCTCTGCGGGGGAGAAGGAGGTACATAAATTACTTCAACCAGCTGACAAGATTTTAGAAACAATGGCTATGGAACTTGCTGAGTATCAGGTCTGCGTTGATATTTTTGTAACTACCCAGGTATACATAGACATAGCTTCTATGTCTGTCATCCCAAGAATCACTGGTGGCCAGGTCTATTATTATTACCCTTTCTCTGCGGTTGCTGACTCTGCAAAGATCTACAATGACCTTAGATGGAATGTCACACGGCCTCAAGGTTTTGAGGCAGTGATGCGTGTGAGATGCAGCCAGGGTCTCCAAGTTCAAGAGTACCATGGGAACTTCTGCAAGCGCGTTCCTACTGACGTTGACCTACCCAGTATTGACTGTGACAAAACTATAACGGTAACCATAAAATATGATGATAAGTTGCAGGATGGCTCAGAATGTGTGTTTCAGTGTGCGCTTCTATACACCACTGTGTACGGTCAAAGAAGAATTCGAGTTACAACGTTAGCTCTGCCATGTACAAGTATACTGAATAATCTTTTCCGTACAGCTGACTTGGATACCCAGTTCGCTTGTATATTGAAACAAGCTGCAATTGAAATTCCTTCTCGCCCGCTCATGCAAGTCCGTGAAAGACTGACTGACCGTTGCATTGGTATTCTGTGTTCTTATAGGAAATTTTGTGCTACAGTTTCATCATCTGGACAACTTGTTCTTCCGGAAACACTTAAGCTCCTACCGTTATACATCCTTGCACTAACTAAAAGCACGGGATTGCGAGCTGAAGGGAAATTAGATGAACGATCCTACTGGATCAATTATGTGTCCTCTGTTTCTACTCCTTTAGCAATAGCACTTGTGTATCCTCGAATGGTGGCAATCCATGATTTAATTTCCAAGACGGATGGATCAATTGTTCCTCCTGCAATTCCACTTTCTAGTGAGCATATCAGCGATGAGGGAATTTATCTTCTTGAGAATGGAGAGGATTGTCTAATATATATTGGCAGCTCGGTAGATCCAGATATCTTGAAGAAGTTGTTCGGTATTTCTTCAGTTGATGAAATTCCTGCTCAGTTTGTGCTGCAGCAGTACGATAATCCATTTTCAGCTAAGCTAAATGACATGGTAAATGAGATTCGACGCCAAAGATGTTCCTACCTCCGCATGAAATTATGCAAGAAAGGAGATCCATCAGGGATGCTCTTCTTCTCGTATATCGTCGAAGATAAAAATTTGAATGGCCTCTCCTATATCGAGTTTCTAATACATATCCATCGGCAGATTCAAATGAAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

1009

Amino Acids

110.07

Weight (kDa)

7.88

Isoelectric Point (pI)

53.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 345 - 383 7.7e-15 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 420 - 660 6e-77 Sec23/Sec24 trunk domain
Sec23_BS PF08033 665 - 749 7.7e-29 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 760 - 858 2.3e-24 Sec23/Sec24 helical domain
Gelsolin PF00626 879 - 948 3.1e-08 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000554)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT4G32640 AT4G32640
fragaria_vesca FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000
malus_domestica MD02G1169200.v1.1 MD13G1230500.v1.1 MD15G1281700.v1.1 MD16G1009900.v1.1 MD16G1235300.v1.1
prunus_persica Prupe.1G056500_v2.0.a1 Prupe.1G056500_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1
pyrus_communis pycom02g13590 pycom13g20290 pycom15g24480 pycom16g00970 pycom16g19630
rosa_chinensis RchiOBHm_Chr4g0396591 RchiOBHm_Chr4g0445631
rosa_laevigata RLG00000005723 RLG00000009518
rosa_multiflora Rmu_co8031254.1_g000001 Rmu_co8351119.1_g000001 Rmu_sc0003637.1_g000024 Rmu_sc0010969.1_g000001 Rmu_sc0019383.1_g000002
rosa_roxburghii Rroxscaffold_5G00341440 Rroxscaffold_5G00386110
rosa_rugosa Rorug03G0348600 Rorug03G0348700 Rorug04G0363200 Rorug04G0363300 Rorug04G0363400 Rorug04G0363500
rosa_samantha Rh4AG070600 Rh4AG424700 Rh4AG425100 Rh4BG068500 Rh4BG436900 Rh4CG076300 Rh4CG451400 Rh4DG065600 Rh4DG433400
rosa_wichuraiana Rw4G005630 Rw4G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 226
Acc16I TGCGCA 1 cut(s) 225
AccB1I GGYRCC 3 cut(s) 367, 457, 1607
AccB7I CCANNNNNTGG 2 cut(s) 973, 1688
AccBSI CCGCTC 1 cut(s) 2341
AccI GTMKAC 2 cut(s) 1857, 2251
AccII CGCG 1 cut(s) 2070
AccIII TCCGGA 1 cut(s) 2443
AclI AACGTT 2 cut(s) 1435, 2228
AclWI GGATC 7 cut(s) 743, 1250, 2523, 2546, 2641, 2738, 2909
AcoI YGGCCR 1 cut(s) 1903
AcsI RAATTY 8 cut(s) 88, 1223, 2214, 2325, 2402, 2688, 2790, 2959
AcuI CTGAAG 5 cut(s) 609, 1389, 1740, 2529, 2763
AcyI GRCGYC 2 cut(s) 1136, 2869
AdeI CACNNNGTG 1 cut(s) 380
AflII CTTAAG 1 cut(s) 2453
AjnI CCWGG 6 cut(s) 10, 376, 535, 1851, 1905, 2025
AjuI GAANNNNNNNTTGG 4 cut(s) 79, 111, 484, 516
AleI CACNNNNGTG 2 cut(s) 583, 2195
AloI GAACNNNNNNTCC 6 cut(s) 2276, 2308, 2421, 2453, 2625, 2657
Alw21I GWGCWC 2 cut(s) 724, 899
Alw26I GTCTC 3 cut(s) 855, 1127, 2036
Alw44I GTGCAC 1 cut(s) 895
AlwI GGATC 7 cut(s) 743, 1250, 2523, 2546, 2641, 2738, 2909
AlwNI CAGNNNCTG 2 cut(s) 632, 1453
Ama87I CYCGRG 2 cut(s) 453, 1199
Aor13HI TCCGGA 1 cut(s) 2443
ApaI GGGCCC 3 cut(s) 209, 293, 399
ApaLI GTGCAC 1 cut(s) 895
ApoI RAATTY 8 cut(s) 88, 1223, 2214, 2325, 2402, 2688, 2790, 2959
ArsI GACNNNNNNTTYG 4 cut(s) 415, 447, 1586, 1618
AseI ATTAAT 1 cut(s) 903
Asp700I GAANNNNTTC 1 cut(s) 2765
AspA2I CCTAGG 1 cut(s) 1376
AspLEI GCGC 3 cut(s) 226, 2070, 2182
AsuC2I CCSGG 4 cut(s) 27, 204, 243, 307
AsuHPI GGTGA 3 cut(s) 162, 315, 1034
AvaI CYCGRG 2 cut(s) 453, 1199
AvaII GGWCC 8 cut(s) 245, 260, 428, 539, 588, 734, 1031, 1163
AvrII CCTAGG 1 cut(s) 1376
BaeGI GKGCMC 5 cut(s) 209, 293, 399, 460, 899
BaeI ACNNNNGTAYC 2 cut(s) 2570, 2603
BalI TGGCCA 1 cut(s) 1905
BanI GGYRCC 3 cut(s) 367, 457, 1607
BanII GRGCYC 5 cut(s) 209, 293, 399, 724, 1702
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 724, 899
BbvCI CCTCAGC 1 cut(s) 480
BccI CCATC 9 cut(s) 997, 1163, 1573, 1690, 1965, 2147, 2624, 2926, 3009
BceAI ACGGC 3 cut(s) 175, 1515, 2000
BciT130I CCWGG 6 cut(s) 12, 378, 537, 1853, 1907, 2027
BciVI GTATCC 2 cut(s) 2278, 2598
BclI TGATCA 1 cut(s) 1087
BcnI CCSGG 4 cut(s) 27, 204, 243, 307
BcoDI GTCTC 3 cut(s) 855, 1127, 2036
BfaI CTAG 3 cut(s) 1377, 1710, 2664
BfmI CTRYAG 7 cut(s) 315, 447, 874, 1108, 1496, 2412, 2810
BfrI CTTAAG 1 cut(s) 2453
BfuI GTATCC 2 cut(s) 2278, 2598
BglII AGATCT 1 cut(s) 1953
BlnI CCTAGG 1 cut(s) 1376
BlpI GCTNAGC 1 cut(s) 2838
Bme18I GGWCC 8 cut(s) 245, 260, 428, 539, 588, 734, 1031, 1163
BmeT110I CYCGRG 2 cut(s) 453, 1199
BmrI ACTGGG 3 cut(s) 876, 2088, 2284
BmsI GCATC 7 cut(s) 135, 1174, 1446, 1569, 1998, 2009, 2916
BmuI ACTGGG 3 cut(s) 876, 2088, 2284
BpiI GAAGAC 1 cut(s) 294
BpmI CTGGAG 4 cut(s) 237, 519, 738, 1335
Bpu10I CCTNAGC 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 2838
BpuEI CTTGAG 2 cut(s) 1974, 2720
BpuMI CCSGG 4 cut(s) 27, 204, 243, 307
BsaAI YACGTR 1 cut(s) 286
BsaBI GATNNNNATC 1 cut(s) 753
BsaHI GRCGYC 2 cut(s) 1136, 2869
BsaI GGTCTC 1 cut(s) 2036
BsaJI CCNNGG 5 cut(s) 11, 1376, 1851, 2026, 2050
BsaWI WCCGGW 1 cut(s) 2443
BsaXI ACNNNNNCTCC 6 cut(s) 706, 736, 811, 841, 2442, 2472
Bse118I RCCGGY 1 cut(s) 364
Bse3DI GCAATG 1 cut(s) 1641
Bse8I GATNNNNATC 1 cut(s) 753
BseAI TCCGGA 1 cut(s) 2443
BseBI CCWGG 6 cut(s) 12, 378, 537, 1853, 1907, 2027
BseDI CCNNGG 5 cut(s) 11, 1376, 1851, 2026, 2050
BseGI GGATG 9 cut(s) 985, 989, 1560, 1565, 1884, 2158, 2472, 2635, 2931
BseJI GATNNNNATC 1 cut(s) 753
BseMI GCAATG 1 cut(s) 1641
BseMII CTCAG 7 cut(s) 494, 798, 1515, 1611, 1806, 2172, 2813
BseRI GAGGAG 5 cut(s) 259, 336, 456, 819, 1482
BseSI GKGCMC 5 cut(s) 209, 293, 399, 460, 899
BsgI GTGCAG 1 cut(s) 569
Bsh1236I CGCG 1 cut(s) 2070
Bsh1285I CGRYCG 1 cut(s) 137
BshNI GGYRCC 3 cut(s) 367, 457, 1607
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 724, 899
BsiHKCI CYCGRG 2 cut(s) 453, 1199
BsiSI CCGG 7 cut(s) 27, 203, 242, 306, 365, 725, 2444
BsiWI CGTACG 1 cut(s) 282
BslFI GGGAC 3 cut(s) 231, 273, 436
BsmAI GTCTC 3 cut(s) 855, 1127, 2036
BsmBI CGTCTC 1 cut(s) 1127
BsmFI GGGAC 3 cut(s) 231, 273, 436
BsmI GAATGC 2 cut(s) 499, 1625
Bso31I GGTCTC 1 cut(s) 2036
BsoBI CYCGRG 2 cut(s) 453, 1199
Bsp120I GGGCCC 3 cut(s) 205, 289, 395
Bsp1286I GDGCHC 7 cut(s) 209, 293, 399, 460, 724, 899, 1702
Bsp13I TCCGGA 1 cut(s) 2443
Bsp1407I TGTACA 3 cut(s) 1474, 1513, 2243
Bsp1720I GCTNAGC 1 cut(s) 2838
Bsp19I CCATGG 1 cut(s) 2050
BspCNI CTCAG 7 cut(s) 493, 797, 1516, 1610, 1807, 2171, 2812
BspEI TCCGGA 1 cut(s) 2443
BspFNI CGCG 1 cut(s) 2070
BspMAI CTGCAG 4 cut(s) 451, 878, 1500, 2814
BspPI GGATC 7 cut(s) 743, 1250, 2523, 2546, 2641, 2738, 2909
BspQI GCTCTTC 2 cut(s) 990, 2936
BspT107I GGYRCC 3 cut(s) 367, 457, 1607
BspTI CTTAAG 1 cut(s) 2453
BspTNI GGTCTC 1 cut(s) 2036
BsrBI CCGCTC 1 cut(s) 2341
BsrDI GCAATG 1 cut(s) 1641
BsrFI RCCGGY 1 cut(s) 364
BsrGI TGTACA 3 cut(s) 1474, 1513, 2243
BssAI RCCGGY 1 cut(s) 364
BssECI CCNNGG 5 cut(s) 11, 1376, 1851, 2026, 2050
BssNAI GTATAC 2 cut(s) 1858, 2252
BssNI GRCGYC 2 cut(s) 1136, 2869
BssT1I CCWWGG 2 cut(s) 1376, 2050
Bst1107I GTATAC 2 cut(s) 1858, 2252
Bst2UI CCWGG 6 cut(s) 12, 378, 537, 1853, 1907, 2027
Bst6I CTCTTC 2 cut(s) 990, 2936
BstACI GRCGYC 2 cut(s) 1136, 2869
BstAFI CTTAAG 1 cut(s) 2453
BstAPI GCANNNNNTGC 1 cut(s) 1337
BstAUI TGTACA 3 cut(s) 1474, 1513, 2243
BstBAI YACGTR 1 cut(s) 286
BstC8I GCNNGC 9 cut(s) 366, 395, 659, 947, 1266, 1333, 2066, 2339, 2505
BstDEI CTNAG 9 cut(s) 480, 784, 1524, 1597, 1815, 1967, 2158, 2799, 2838
BstDSI CCRYGG 1 cut(s) 2050
BstEII GGTNACC 1 cut(s) 2122
BstF5I GGATG 9 cut(s) 985, 989, 1560, 1565, 1884, 2158, 2472, 2635, 2931
BstFNI CGCG 1 cut(s) 2070
BstHHI GCGC 3 cut(s) 226, 2070, 2182
BstMAI GTCTC 3 cut(s) 855, 1127, 2036
BstMCI CGRYCG 1 cut(s) 137
BstNI CCWGG 6 cut(s) 12, 378, 537, 1853, 1907, 2027
BstNSI RCATGY 4 cut(s) 661, 704, 949, 1335
BstPI GGTNACC 1 cut(s) 2122
BstSFI CTRYAG 7 cut(s) 315, 447, 874, 1108, 1496, 2412, 2810
BstSLI GKGCMC 5 cut(s) 209, 293, 399, 460, 899
BstUI CGCG 1 cut(s) 2070
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 3 cut(s) 1953, 2743, 2914
BstXI CCANNNNNNTGG 1 cut(s) 585
BstYI RGATCY 3 cut(s) 1953, 2743, 2914
BstZ17I GTATAC 2 cut(s) 1858, 2252
BsuI GTATCC 2 cut(s) 2278, 2598
BtgI CCRYGG 1 cut(s) 2050
BtgZI GCGATG 1 cut(s) 2694
BtsCI GGATG 9 cut(s) 985, 989, 1560, 1565, 1884, 2158, 2472, 2635, 2931
BtsI GCAGTG 4 cut(s) 918, 1150, 1342, 2010
Cac8I GCNNGC 9 cut(s) 366, 395, 659, 947, 1266, 1333, 2066, 2339, 2505
CaiI CAGNNNCTG 2 cut(s) 632, 1453
CfoI GCGC 3 cut(s) 226, 2070, 2182
Cfr10I RCCGGY 1 cut(s) 364
CseI GACGC 3 cut(s) 618, 1144, 2877
CsiI ACCWGGT 1 cut(s) 376
CspCI CAANNNNNGTGG 2 cut(s) 651, 686
DdeI CTNAG 9 cut(s) 480, 784, 1524, 1597, 1815, 1967, 2158, 2799, 2838
DraIII CACNNNGTG 1 cut(s) 380
DrdI GACNNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 1903
Eam1104I CTCTTC 2 cut(s) 990, 2936
EarI CTCTTC 2 cut(s) 990, 2936
EciI GGCGGA 3 cut(s) 477, 531, 822
Ecl136II GAGCTC 1 cut(s) 722
Eco130I CCWWGG 2 cut(s) 1376, 2050
Eco24I GRGCYC 5 cut(s) 209, 293, 399, 724, 1702
Eco31I GGTCTC 1 cut(s) 2036
Eco32I GATATC 1 cut(s) 2752
Eco47I GGWCC 8 cut(s) 245, 260, 428, 539, 588, 734, 1031, 1163
Eco53kI GAGCTC 1 cut(s) 722
Eco57I CTGAAG 5 cut(s) 609, 1389, 1740, 2529, 2763
Eco88I CYCGRG 2 cut(s) 453, 1199
Eco91I GGTNACC 1 cut(s) 2122
EcoICRI GAGCTC 1 cut(s) 722
EcoO109I RGGNCCY 4 cut(s) 245, 260, 396, 1373
EcoO65I GGTNACC 1 cut(s) 2122
EcoRI GAATTC 1 cut(s) 2214
EcoRII CCWGG 6 cut(s) 10, 376, 535, 1851, 1905, 2025
EcoRV GATATC 1 cut(s) 2752
EcoT14I CCWWGG 2 cut(s) 1376, 2050
EcoT22I ATGCAT 2 cut(s) 501, 597
EcoT38I GRGCYC 5 cut(s) 209, 293, 399, 724, 1702
ErhI CCWWGG 2 cut(s) 1376, 2050
Esp3I CGTCTC 1 cut(s) 1127
FaqI GGGAC 3 cut(s) 231, 273, 436
FauI CCCGC 5 cut(s) 369, 386, 413, 1735, 2346
FbaI TGATCA 1 cut(s) 1087
FblI GTMKAC 2 cut(s) 1857, 2251
FokI GGATG 9 cut(s) 972, 976, 1547, 1552, 1871, 2165, 2459, 2642, 2938
FriOI GRGCYC 5 cut(s) 209, 293, 399, 724, 1702
FspBI CTAG 3 cut(s) 1377, 1710, 2664
FspI TGCGCA 1 cut(s) 225
GlaI GCGC 3 cut(s) 225, 2069, 2181
GsuI CTGGAG 4 cut(s) 237, 519, 738, 1335
HapII CCGG 7 cut(s) 27, 203, 242, 306, 365, 725, 2444
HgaI GACGC 3 cut(s) 618, 1144, 2877
HhaI GCGC 3 cut(s) 226, 2070, 2182
Hin1I GRCGYC 2 cut(s) 1136, 2869
Hin6I GCGC 3 cut(s) 224, 2068, 2180
HinP1I GCGC 3 cut(s) 224, 2068, 2180
HincII GTYRAC 2 cut(s) 1535, 2086
HindII GTYRAC 2 cut(s) 1535, 2086
HinfI GANTC 8 cut(s) 95, 124, 754, 1595, 1893, 1943, 2863, 3010
HpaII CCGG 7 cut(s) 27, 203, 242, 306, 365, 725, 2444
HphI GGTGA 3 cut(s) 162, 315, 1034
Hpy166II GTNNAC 8 cut(s) 38, 881, 897, 1535, 1858, 2086, 2200, 2252
Hpy188I TCNGA 9 cut(s) 94, 106, 219, 753, 860, 994, 1360, 1525, 2161
Hpy8I GTNNAC 8 cut(s) 38, 881, 897, 1535, 1858, 2086, 2200, 2252
Hpy99I CGWCG 2 cut(s) 2871, 2951
HpyCH4IV ACGT 5 cut(s) 285, 584, 1435, 2082, 2228
HpyF3I CTNAG 9 cut(s) 480, 784, 1524, 1597, 1815, 1967, 2158, 2799, 2838
HpySE526I ACGT 5 cut(s) 285, 584, 1435, 2082, 2228
Hsp92I GRCGYC 2 cut(s) 1136, 2869
HspAI GCGC 3 cut(s) 224, 2068, 2180
KflI GGGWCCC 2 cut(s) 245, 260
Kpn2I TCCGGA 1 cut(s) 2443
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 1087
LguI GCTCTTC 2 cut(s) 990, 2936
LmnI GCTCC 7 cut(s) 13, 719, 727, 969, 1316, 1697, 2463
LweI GCATC 7 cut(s) 135, 1174, 1446, 1569, 1998, 2009, 2916
MabI ACCWGGT 1 cut(s) 376
MaeI CTAG 3 cut(s) 1377, 1710, 2664
MaeII ACGT 5 cut(s) 285, 584, 1435, 2082, 2228
MaeIII GTNAC 5 cut(s) 1843, 1978, 2105, 2122, 2221
MbiI CCGCTC 1 cut(s) 2341
MfeI CAATTG 3 cut(s) 1686, 2319, 2637
MflI RGATCY 3 cut(s) 1953, 2743, 2914
MhlI GDGCHC 7 cut(s) 209, 293, 399, 460, 724, 899, 1702
MlsI TGGCCA 1 cut(s) 1905
MluNI TGGCCA 1 cut(s) 1905
MlyI GAGTC 2 cut(s) 1589, 1937
MmeI TCCRAC 2 cut(s) 84, 991
Mox20I TGGCCA 1 cut(s) 1905
Mph1103I ATGCAT 2 cut(s) 501, 597
MroI TCCGGA 1 cut(s) 2443
MroNI GCCGGC 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 2765
MscI TGGCCA 1 cut(s) 1905
MseI TTAA 3 cut(s) 903, 2454, 2615
MslI CAYNNNNRTG 2 cut(s) 583, 2195
Msp20I TGGCCA 1 cut(s) 1905
MspA1I CMGCKG 4 cut(s) 613, 1592, 1778, 2276
MspCI CTTAAG 1 cut(s) 2453
MspI CCGG 7 cut(s) 27, 203, 242, 306, 365, 725, 2444
MunI CAATTG 3 cut(s) 1686, 2319, 2637
Mva1269I GAATGC 2 cut(s) 499, 1625
MvaI CCWGG 6 cut(s) 12, 378, 537, 1853, 1907, 2027
MvnI CGCG 1 cut(s) 2070
NaeI GCCGGC 1 cut(s) 366
NciI CCSGG 4 cut(s) 27, 204, 243, 307
NcoI CCATGG 1 cut(s) 2050
NgoMIV GCCGGC 1 cut(s) 364
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 2 cut(s) 1978, 2105
NsbI TGCGCA 1 cut(s) 225
NsiI ATGCAT 2 cut(s) 501, 597
NspI RCATGY 4 cut(s) 661, 704, 949, 1335
OliI CACNNNNGTG 2 cut(s) 583, 2195
PaeI GCATGC 3 cut(s) 661, 949, 1335
PciSI GCTCTTC 2 cut(s) 990, 2936
PcsI WCGNNNNNNNCGW 3 cut(s) 278, 1045, 1197
PctI GAATGC 2 cut(s) 499, 1625
PdiI GCCGGC 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 2765
PfeI GAWTC 6 cut(s) 95, 124, 754, 1893, 2863, 3010
Pfl23II CGTACG 1 cut(s) 282
PflMI CCANNNNNTGG 2 cut(s) 973, 1688
PleI GAGTC 2 cut(s) 1589, 1937
PpsI GAGTC 2 cut(s) 1589, 1937
Ppu21I YACGTR 1 cut(s) 286
PpuMI RGGWCCY 2 cut(s) 245, 260
PshBI ATTAAT 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 724
Psp1406I AACGTT 2 cut(s) 1435, 2228
Psp5II RGGWCCY 2 cut(s) 245, 260
Psp6I CCWGG 6 cut(s) 10, 376, 535, 1851, 1905, 2025
PspEI GGTNACC 1 cut(s) 2122
PspGI CCWGG 6 cut(s) 10, 376, 535, 1851, 1905, 2025
PspLI CGTACG 1 cut(s) 282
PspOMI GGGCCC 3 cut(s) 205, 289, 395
PspPPI RGGWCCY 2 cut(s) 245, 260
PsrI GAACNNNNNNTAC 2 cut(s) 2374, 2406
PstI CTGCAG 4 cut(s) 451, 878, 1500, 2814
PstNI CAGNNNCTG 2 cut(s) 632, 1453
PsuI RGATCY 3 cut(s) 1953, 2743, 2914
PvuII CAGCTG 4 cut(s) 613, 1592, 1778, 2276
RseI CAYNNNNRTG 2 cut(s) 583, 2195
SacI GAGCTC 1 cut(s) 724
SapI GCTCTTC 2 cut(s) 990, 2936
SaqAI TTAA 3 cut(s) 903, 2454, 2615
SchI GAGTC 2 cut(s) 1589, 1937
SduI GDGCHC 7 cut(s) 209, 293, 399, 460, 724, 899, 1702
SexAI ACCWGGT 1 cut(s) 376
SfaNI GCATC 7 cut(s) 135, 1174, 1446, 1569, 1998, 2009, 2916
SfcI CTRYAG 7 cut(s) 315, 447, 874, 1108, 1496, 2412, 2810
SinI GGWCC 8 cut(s) 245, 260, 428, 539, 588, 734, 1031, 1163
SmiMI CAYNNNNRTG 2 cut(s) 583, 2195
SmlI CTYRAG 3 cut(s) 1989, 2453, 2699
SmoI CTYRAG 3 cut(s) 1989, 2453, 2699
SphI GCATGC 3 cut(s) 661, 949, 1335
SspMI CTAG 3 cut(s) 1377, 1710, 2664
SstI GAGCTC 1 cut(s) 724
StyI CCWWGG 2 cut(s) 1376, 2050
TaiI ACGT 5 cut(s) 288, 587, 1438, 2085, 2231
TaqI TCGA 7 cut(s) 133, 143, 2218, 2593, 2866, 2949, 2982
TatI WGTACW 4 cut(s) 1242, 1474, 1513, 2243
TauI GCSGC 7 cut(s) 556, 559, 619, 731, 1328, 1331, 1613
TfiI GAWTC 6 cut(s) 95, 124, 754, 1893, 2863, 3010
Tru1I TTAA 3 cut(s) 903, 2454, 2615
Tru9I TTAA 3 cut(s) 903, 2454, 2615
TseFI GTSAC 2 cut(s) 1978, 2105
Tsp45I GTSAC 2 cut(s) 1978, 2105
TspGWI ACGGA 3 cut(s) 2258, 2342, 2642
Van91I CCANNNNNTGG 2 cut(s) 973, 1688
Vha464I CTTAAG 1 cut(s) 2453
VneI GTGCAC 1 cut(s) 895
VpaK11BI GGWCC 8 cut(s) 245, 260, 428, 539, 588, 734, 1031, 1163
VspI ATTAAT 1 cut(s) 903
XapI RAATTY 8 cut(s) 88, 1223, 2214, 2325, 2402, 2688, 2790, 2959
XceI RCATGY 4 cut(s) 661, 704, 949, 1335
XcmI CCANNNNNNNNNTGG 1 cut(s) 1157
XmaJI CCTAGG 1 cut(s) 1376
XmiI GTMKAC 2 cut(s) 1857, 2251
XmnI GAANNNNTTC 1 cut(s) 2765
XspI CTAG 3 cut(s) 1377, 1710, 2664
Zsp2I ATGCAT 2 cut(s) 501, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.