MD16G1235300.v1.1

Protein transport protein Sec24-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000554
Physical Location & Seq
Forward (+)
24206465 .. 24221206
14742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1235300.v1.1.491

Sequence Viewer

Length: 3231 bp
ATGCAGCCCCGCCCTGATTTATTAGCCGATAATATCCAGAATATGAATCTTAATCGGCCGCCTTCCATGCCTAATTCTGCCCCTCCTAGACCATCCCCTTTCGGCCAACAGCCCCCTTTTCCTTCAACAGCCCCTCCTGCCGGTGCCGGGGCTCCTTCTGCACCACCTCCTTTTTCTCGGCCCGGCCCTCCCCCGGCTGCCTTTGCAAGACCCGCAGCACCAGCACCGCGATCGGGACCCCCGCAACCAACACTATCCCCAGCCACCACCCCAGTAAGACCCGCCGGGCCGCCGGTTGGCCAGCCTTCATCTTTCCCGTCTCGACCGCCTCCTGGGTCGTTTCCCCCTGTGGGCGGGGTAGCTCCTGCTTCTGGGCCACCACCAATTGGTCCAATTGGTCCGGCATCAGGTCCTCCCCCAGGTCTTCAGAGCATGCCGCCTACGACTGCACCGGGTAGAATGTTGAGTAATGGGCCACCAATGTTTGGTTACGGTGCTATGCCGGGTGGACCTCGTTTCCCTCCCAGTGGAAATGCACCTCAACCACCTGTTGGACATCCACCAGCAATGGCACCAGCACCAGCCGCGGGTCCACCACGAACCCCATCCATGCACTCCGTGCTGGGTGGTTCGGCAGTTAGTTCTCCACCAGGTCCTACTGTTCAGCAGCCACCACCATTGTCGGCAGCCCCGCCATTTTCAGCTCCACCTCAAAGCATGCGGCCACCAGCTTCAGGCTCTCCCTATGGCTCACAGACATGGCCAGTGCAACAAGGGCAGGTGGCTCCACCTTTGCAATTTCCTGGTTCTGCTCAACCACCTAGAATGTTTGGAATGCCACCCCCACCACTACCAAATCAGTCCATGACTACCATCTCACCTGCTGGTCAAACTGGTACTCCTTTGGCTGGGTCATCCAAGATTGATCCCACTCAAATTCCTCGGCCTATACCAAGTTCATCGATGCTCATTCATGAAACTCGTCAGGGCAATCAGGCGAATCCACCTCCGCCTGCTACAACTGATTATATTGTCAGAGACACTGGGAATTGCAGTCCACGTTACATGAGGTGCACCATCAATCAGATACCATGCACTGCAGATCTTTTAACGACGTCAGGAATGCCATTAGCTTTGTTGGTTGAACCTTTTGCCCTTCCTCATCCAAATGAGGAACCAATCCAAGTTGTGGATTTCGGTGAAAGTGGTCCTGTTCGATGCTCTCGTTGCAAAGGCTACATAAATCCTTTCATGAAGTTCATTGACCAGGGCAGGAAATTCATCTGTAATCTTTGTGGATTTACCGATGAGACTCCGCGTGACTACCACTGCAATCTTGGTCCAGATGGTAGACGCAGAGATGCTGATGAGAGGCCTGAGCTATGTAGAGGAACAGTTGAATTTGTTGCTTCAAAAGAATACATGGTACGTGATCCCATGCCAGCTGTGTACTTCTTCCTCATTGATGTATCCATGAATGCCATTCAAACTGGTGCAACAGCTGCAGCTTGCAGTGCACTCAGTCAAGTTATTGCTGATCTTCCGTCAATTTGTGAGTGTGAAGGTCCTCGGACAATGGTGGGAATTGCAACATTTGACTCAACGATCCATTTTTACAATTTGAAACGTGCACTGCAGCAGCCTTTGATGCTTATTGTTCCTGATGTGCAAGATGTTTACACTCCTCTGGAGACTGATGTAGTGGTCCAGCTTTCCGAGTGCCGCCAACATTTGCAGCAATTGTTGGAAAGTATTCCAACCATGTTTCAAAACGGTAAAACTGCTGAATCAGCCTTTGGTGCGGCAATCAAGGCTGCTTTCTTGGCAATTAAGAGTACTGGAGGGAAACTTTTGGTGTTTCAGTCAGTGTTGGCATCAACTGGAATTGGAGCGCTCTCTGCTAGAGAGGCCGAAGGAAGAGCAAATATATCCTCAGCTGAGAAGGAGGCCCATAAATTACTCCAACCAGCTGACAAAACTTTAAAGACAATGGCAATTGAGTTTGCTGAATATCAAGTTTGTGTTGACTTGTTTATCACTACTCAGTCATACATAGACATTGCTTCTATCTCTGTTATCCCAAGAACAACTGGAGGGCAGCTTTACTATTACTACCCCTTCTCAGCTGTCTCTGATCCTGCAAAGCTCTACAACGATCTTAGATGGAATGTCACAAGGCCTCAAGGTTTTGAGGCGGTGATGCGTGTAAGATGCAGCCAGGGTATTCAAGTTCAAGAGTACCATGGAAGCTTTTGCAAACGTATTCCTACGGATGTTGACCTACCCGGGATTGACTGTGACAAAACTATTATGGTGACCTTAAAACATGATGATAAATTACAGGATGGCTCAGAATGTGGTTTCCAGTGTGCCGTTTTATACACAACTGTGTATGGCCAACGGAGAATTCGAGTTGCAACTTTATCTCTGCCATGCACTAGTATGCTAAGTAATCTGTTCCGCGCTGCTGATTTGGACACTCAATTTGCATGTTTCATGAAGCAAGCGGCAAATGAAATTCCTTCAAGTCACCTCTTGCATGTACGGGAACAAGTGACAAATCTGTGCATTAGTAGTCTGCTTTCATATCGTAAATTTTGTGCTACTGTATCATCTTCTGGACAGCTTATTTTACCGGAGGCACTGAAGCTCCTGCCTCTATACACCCTTGCATTAATCAAAAGTGTGGGGCTACGAACTGATGGAAAAATAGATGAGCGATCCTTCTGGATCAACCATGTTTCTTCCCTTTCGGTTCCTTTGGCTGTACCACTAGTGTACCCGAGAATGATGGCTATTCATGACCTTGAATCCAAAAGGGAGGGAGATGATTCTCTAATACCTCCGGTGATTCCGCTTTCTAGCGAACACGTGCGTAACGAGGGAATTTATCTTCTCGAGAATGGTGACGACTGTTTAATATATATTGGGAATTTGGTGGATTCTGGAATTTTGCAACAACTGTTTGGAATCACCTCTGCTGATGAACTTCCCACACAGTTTGTGCTACAGCAGTATGATAATCCATTATCAAAGAAGCTGAATGATGCGGTAAATGAAATACGGCGCCAGAGATGTTCCTACCTTCGCTTAAAATTATGTAAGAAAGGAGATCCATCAGGAACATTGTTTTTCTCATATATGGTCGAAGACCAGAGTCCGAATGGCCCCTCCTACGTTGAATTTCTGGTGCATGTCCATCGGCAAATACAGATAAAAATGGCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

1077

Amino Acids

115.78

Weight (kDa)

7.45

Isoelectric Point (pI)

56.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 404 - 442 1.5e-16 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 479 - 724 3.7e-77 Sec23/Sec24 trunk domain
Sec23_BS PF08033 729 - 813 7.7e-26 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 824 - 922 2.3e-24 Sec23/Sec24 helical domain
Gelsolin PF00626 948 - 1015 1.1e-07 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000554)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT4G32640 AT4G32640
fragaria_vesca FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000
malus_domestica MD02G1169200.v1.1 MD13G1230500.v1.1 MD15G1281700.v1.1 MD16G1009900.v1.1 MD16G1235300.v1.1
prunus_persica Prupe.1G056500_v2.0.a1 Prupe.1G056500_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1
pyrus_communis pycom02g13590 pycom13g20290 pycom15g24480 pycom16g00970 pycom16g19630
rosa_chinensis RchiOBHm_Chr4g0396591 RchiOBHm_Chr4g0445631
rosa_laevigata RLG00000005723 RLG00000009518
rosa_multiflora Rmu_co8031254.1_g000001 Rmu_co8351119.1_g000001 Rmu_sc0003637.1_g000024 Rmu_sc0010969.1_g000001 Rmu_sc0019383.1_g000002
rosa_roxburghii Rroxscaffold_5G00341440 Rroxscaffold_5G00386110
rosa_rugosa Rorug03G0348600 Rorug03G0348700 Rorug04G0363200 Rorug04G0363300 Rorug04G0363400 Rorug04G0363500
rosa_samantha Rh4AG070600 Rh4AG424700 Rh4AG425100 Rh4BG068500 Rh4BG436900 Rh4CG076300 Rh4CG451400 Rh4DG065600 Rh4DG433400
rosa_wichuraiana Rw4G005630 Rw4G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 769, 889
AatII GACGTC 1 cut(s) 1118
Acc36I ACCTGC 2 cut(s) 769, 889
AccB1I GGYRCC 3 cut(s) 143, 571, 3066
AccB7I CCANNNNNTGG 4 cut(s) 386, 485, 551, 1189
AccI GTMKAC 1 cut(s) 1351
AccII CGCG 4 cut(s) 229, 587, 1318, 2463
AclWI GGATC 7 cut(s) 920, 1427, 1600, 2129, 2715, 2738, 3107
AcoI YGGCCR 6 cut(s) 56, 103, 298, 722, 761, 2395
AcuI CTGAAG 3 cut(s) 410, 717, 2666
AcvI CACGTG 1 cut(s) 2872
AcyI GRCGYC 2 cut(s) 1115, 3067
AdeI CACNNNGTG 1 cut(s) 619
AfaI GTAC 8 cut(s) 898, 1428, 1451, 1837, 2240, 2544, 2769, 2780
AfeI AGCGCT 1 cut(s) 1893
AflIII ACRYGT 1 cut(s) 2869
AhlI ACTAGT 2 cut(s) 2438, 2773
AjnI CCWGG 6 cut(s) 331, 418, 649, 802, 1266, 2217
AjuI GAANNNNNNNTTGG 4 cut(s) 1779, 1811, 2391, 2423
AleI CACNNNNGTG 1 cut(s) 2387
Alw21I GWGCWC 3 cut(s) 1076, 1519, 1633
Alw26I GTCTC 5 cut(s) 324, 1032, 1304, 1685, 2134
Alw44I GTGCAC 3 cut(s) 1072, 1515, 1629
AlwI GGATC 7 cut(s) 920, 1427, 1600, 2129, 2715, 2738, 3107
Ama87I CYCGRG 3 cut(s) 2285, 2782, 2897
Aor51HI AGCGCT 1 cut(s) 1893
ApaLI GTGCAC 3 cut(s) 1072, 1515, 1629
ArsI GACNNNNNNTTYG 2 cut(s) 2468, 2500
AseI ATTAAT 1 cut(s) 2675
Asp700I GAANNNNTTC 1 cut(s) 1752
AspLEI GCGC 3 cut(s) 1894, 2465, 3069
AsuC2I CCSGG 8 cut(s) 148, 183, 194, 286, 453, 504, 2286, 2287
AsuHPI GGTGA 8 cut(s) 870, 1211, 2209, 2326, 2522, 2860, 2918, 2965
AvaI CYCGRG 3 cut(s) 2285, 2782, 2897
BaeGI GKGCMC 3 cut(s) 1076, 1519, 1633
BaeI ACNNNNGTAYC 2 cut(s) 2762, 2795
BalI TGGCCA 3 cut(s) 300, 763, 2397
BanI GGYRCC 3 cut(s) 143, 571, 3066
BanII GRGCYC 1 cut(s) 154
BbrPI CACGTG 1 cut(s) 2872
BbsI GAAGAC 2 cut(s) 416, 3156
Bbv12I GWGCWC 3 cut(s) 1076, 1519, 1633
BbvCI CCTCAGC 1 cut(s) 1933
BceAI ACGGC 2 cut(s) 2357, 3080
BcgI CGANNNNNNTGC 4 cut(s) 213, 247, 3182, 3216
BciT130I CCWGG 6 cut(s) 333, 420, 651, 804, 1268, 2219
BciVI GTATCC 1 cut(s) 1480
BcnI CCSGG 8 cut(s) 148, 183, 194, 286, 453, 504, 2286, 2287
BcoDI GTCTC 5 cut(s) 324, 1032, 1304, 1685, 2134
BcuI ACTAGT 2 cut(s) 2438, 2773
BfaI CTAG 6 cut(s) 87, 822, 1902, 2439, 2774, 2862
BfmI CTRYAG 4 cut(s) 1098, 1503, 1634, 3008
BfoI RGCGCY 2 cut(s) 1895, 3070
BfuAI ACCTGC 2 cut(s) 769, 889
BfuI GTATCC 1 cut(s) 1480
BglII AGATCT 1 cut(s) 1102
BmcAI AGTACT 1 cut(s) 1837
BmeT110I CYCGRG 3 cut(s) 2285, 2782, 2897
BmrI ACTGGG 3 cut(s) 266, 519, 1053
BmsI GCATC 9 cut(s) 413, 954, 1208, 1351, 1638, 1883, 2190, 2201, 3037
BmuI ACTGGG 3 cut(s) 266, 519, 1053
BoxI GACNNNNGTC 1 cut(s) 3156
BpiI GAAGAC 2 cut(s) 416, 3156
BpmI CTGGAG 3 cut(s) 1709, 1860, 2112
Bpu10I CCTNAGC 2 cut(s) 1377, 1933
BpuEI CTTGAG 1 cut(s) 2166
BpuMI CCSGG 8 cut(s) 148, 183, 194, 286, 453, 504, 2286, 2287
Bsa29I ATCGAT 1 cut(s) 962
BsaAI YACGTR 2 cut(s) 1430, 2872
BsaHI GRCGYC 2 cut(s) 1115, 3067
BsaWI WCCGGW 2 cut(s) 2635, 2845
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 883, 913, 2634, 2664
Bse118I RCCGGY 2 cut(s) 140, 292
Bse3DI GCAATG 2 cut(s) 573, 2058
BseBI CCWGG 6 cut(s) 333, 420, 651, 804, 1268, 2219
BseCI ATCGAT 1 cut(s) 962
BseGI GGATG 7 cut(s) 92, 556, 605, 914, 1162, 2278, 2350
BseMI GCAATG 2 cut(s) 573, 2058
BseMII CTCAG 7 cut(s) 1368, 1534, 1929, 1947, 2057, 2136, 2364
BseRI GAGGAG 1 cut(s) 1674
BseSI GKGCMC 3 cut(s) 1076, 1519, 1633
BseX3I CGGCCG 1 cut(s) 56
BseYI CCCAGC 3 cut(s) 259, 622, 908
BsgI GTGCAG 2 cut(s) 144, 432
Bsh1236I CGCG 4 cut(s) 229, 587, 1318, 2463
Bsh1285I CGRYCG 3 cut(s) 59, 233, 326
BshNI GGYRCC 3 cut(s) 143, 571, 3066
BshVI ATCGAT 1 cut(s) 962
BsiEI CGRYCG 3 cut(s) 59, 233, 326
BsiHKAI GWGCWC 3 cut(s) 1076, 1519, 1633
BsiHKCI CYCGRG 3 cut(s) 2285, 2782, 2897
BslFI GGGAC 1 cut(s) 249
BsmAI GTCTC 5 cut(s) 324, 1032, 1304, 1685, 2134
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 249
BsmI GAATGC 3 cut(s) 840, 1128, 1483
BsoBI CYCGRG 3 cut(s) 2285, 2782, 2897
Bsp1286I GDGCHC 4 cut(s) 154, 1076, 1519, 1633
Bsp19I CCATGG 1 cut(s) 2242
BspCNI CTCAG 7 cut(s) 1369, 1533, 1930, 1946, 2056, 2135, 2363
BspDI ATCGAT 1 cut(s) 962
BspFNI CGCG 4 cut(s) 229, 587, 1318, 2463
BspHI TCATGA 4 cut(s) 973, 1251, 2496, 2800
BspMAI CTGCAG 3 cut(s) 1102, 1507, 1638
BspMI ACCTGC 2 cut(s) 769, 889
BspPI GGATC 7 cut(s) 920, 1427, 1600, 2129, 2715, 2738, 3107
BspQI GCTCTTC 1 cut(s) 1912
BspT107I GGYRCC 3 cut(s) 143, 571, 3066
BsrDI GCAATG 2 cut(s) 573, 2058
BsrFI RCCGGY 2 cut(s) 140, 292
BssAI RCCGGY 2 cut(s) 140, 292
BssNI GRCGYC 2 cut(s) 1115, 3067
BssT1I CCWWGG 1 cut(s) 2242
Bst2UI CCWGG 6 cut(s) 333, 420, 651, 804, 1268, 2219
Bst6I CTCTTC 1 cut(s) 1912
BstACI GRCGYC 2 cut(s) 1115, 3067
BstAPI GCANNNNNTGC 2 cut(s) 619, 1502
BstBAI YACGTR 2 cut(s) 1430, 2872
BstC8I GCNNGC 7 cut(s) 302, 434, 719, 1014, 1443, 1510, 2505
BstDEI CTNAG 9 cut(s) 1377, 1520, 1933, 1938, 2043, 2122, 2159, 2350, 2447
BstDSI CCRYGG 2 cut(s) 585, 2242
BstEII GGTNACC 1 cut(s) 2314
BstENI CCTNNNNNAGG 1 cut(s) 417
BstF5I GGATG 7 cut(s) 92, 556, 605, 914, 1162, 2278, 2350
BstFNI CGCG 4 cut(s) 229, 587, 1318, 2463
BstH2I RGCGCY 2 cut(s) 1895, 3070
BstHHI GCGC 3 cut(s) 1894, 2465, 3069
BstMAI GTCTC 5 cut(s) 324, 1032, 1304, 1685, 2134
BstMCI CGRYCG 3 cut(s) 59, 233, 326
BstNI CCWGG 6 cut(s) 333, 420, 651, 804, 1268, 2219
BstNSI RCATGY 5 cut(s) 436, 721, 2493, 2543, 3197
BstPAI GACNNNNGTC 1 cut(s) 3156
BstPI GGTNACC 1 cut(s) 2314
BstSFI CTRYAG 4 cut(s) 1098, 1503, 1634, 3008
BstSLI GKGCMC 3 cut(s) 1076, 1519, 1633
BstUI CGCG 4 cut(s) 229, 587, 1318, 2463
BstV2I GAAGAC 2 cut(s) 416, 3156
BstX2I RGATCY 2 cut(s) 1102, 3112
BstYI RGATCY 2 cut(s) 1102, 3112
BstZI CGGCCG 1 cut(s) 56
Bsu15I ATCGAT 1 cut(s) 962
BsuI GTATCC 1 cut(s) 1480
BsuTUI ATCGAT 1 cut(s) 962
BtgI CCRYGG 2 cut(s) 585, 2242
BtsCI GGATG 7 cut(s) 92, 556, 605, 914, 1162, 2278, 2350
BtsI GCAGTG 4 cut(s) 1095, 1327, 1519, 1631
BveI ACCTGC 2 cut(s) 769, 889
Cac8I GCNNGC 7 cut(s) 302, 434, 719, 1014, 1443, 1510, 2505
CciI TCATGA 4 cut(s) 973, 1251, 2496, 2800
CfoI GCGC 3 cut(s) 1894, 2465, 3069
Cfr10I RCCGGY 2 cut(s) 140, 292
Cfr42I CCGCGG 1 cut(s) 588
Cfr9I CCCGGG 1 cut(s) 2285
ClaI ATCGAT 1 cut(s) 962
CseI GACGC 1 cut(s) 1362
CsiI ACCWGGT 1 cut(s) 649
Csp6I GTAC 8 cut(s) 897, 1427, 1450, 1836, 2239, 2543, 2768, 2779
CspCI CAANNNNNGTGG 4 cut(s) 660, 695, 2983, 3018
CviQI GTAC 8 cut(s) 897, 1427, 1450, 1836, 2239, 2543, 2768, 2779
DdeI CTNAG 9 cut(s) 1377, 1520, 1933, 1938, 2043, 2122, 2159, 2350, 2447
DinI GGCGCC 1 cut(s) 3068
DraI TTTAAA 1 cut(s) 1983
DraIII CACNNNGTG 1 cut(s) 619
EaeI YGGCCR 6 cut(s) 56, 103, 298, 722, 761, 2395
EagI CGGCCG 1 cut(s) 56
Eam1104I CTCTTC 1 cut(s) 1912
EarI CTCTTC 1 cut(s) 1912
EciI GGCGGA 1 cut(s) 999
EclXI CGGCCG 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 2242
Eco147I AGGCCT 2 cut(s) 1375, 2179
Eco24I GRGCYC 1 cut(s) 154
Eco47III AGCGCT 1 cut(s) 1893
Eco52I CGGCCG 1 cut(s) 56
Eco57I CTGAAG 3 cut(s) 410, 717, 2666
Eco72I CACGTG 1 cut(s) 2872
Eco88I CYCGRG 3 cut(s) 2285, 2782, 2897
Eco91I GGTNACC 1 cut(s) 2314
EcoNI CCTNNNNNAGG 1 cut(s) 417
EcoO109I RGGNCCY 4 cut(s) 236, 410, 653, 1565
EcoO65I GGTNACC 1 cut(s) 2314
EcoRI GAATTC 1 cut(s) 2406
EcoRII CCWGG 6 cut(s) 331, 418, 649, 802, 1266, 2217
EcoT14I CCWWGG 1 cut(s) 2242
EcoT38I GRGCYC 1 cut(s) 154
EgeI GGCGCC 1 cut(s) 3068
EheI GGCGCC 1 cut(s) 3068
ErhI CCWWGG 1 cut(s) 2242
Esp3I CGTCTC 1 cut(s) 324
FaqI GGGAC 1 cut(s) 249
FauI CCCGC 7 cut(s) 17, 220, 249, 289, 347, 580, 699
FblI GTMKAC 1 cut(s) 1351
FokI GGATG 7 cut(s) 79, 543, 592, 901, 1149, 2285, 2357
FriOI GRGCYC 1 cut(s) 154
FspBI CTAG 6 cut(s) 87, 822, 1902, 2439, 2774, 2862
GlaI GCGC 3 cut(s) 1893, 2464, 3068
GsaI CCCAGC 3 cut(s) 263, 626, 912
GsuI CTGGAG 3 cut(s) 1709, 1860, 2112
HaeII RGCGCY 2 cut(s) 1895, 3070
HgaI GACGC 1 cut(s) 1362
HhaI GCGC 3 cut(s) 1894, 2465, 3069
Hin1I GRCGYC 2 cut(s) 1115, 3067
Hin6I GCGC 3 cut(s) 1892, 2463, 3067
HinP1I GCGC 3 cut(s) 1892, 2463, 3067
HincII GTYRAC 2 cut(s) 2026, 2278
HindII GTYRAC 2 cut(s) 2026, 2278
HindIII AAGCTT 1 cut(s) 2248
HphI GGTGA 8 cut(s) 870, 1211, 2209, 2326, 2522, 2860, 2918, 2965
Hpy188I TCNGA 8 cut(s) 429, 1037, 1086, 1572, 1717, 2134, 2353, 3162
Hpy99I CGWCG 1 cut(s) 1117
HpyCH4IV ACGT 7 cut(s) 1060, 1115, 1429, 1627, 2260, 2871, 3177
HpyF3I CTNAG 9 cut(s) 1377, 1520, 1933, 1938, 2043, 2122, 2159, 2350, 2447
HpySE526I ACGT 7 cut(s) 1060, 1115, 1429, 1627, 2260, 2871, 3177
Hsp92I GRCGYC 2 cut(s) 1115, 3067
HspAI GCGC 3 cut(s) 1892, 2463, 3067
KasI GGCGCC 1 cut(s) 3066
KflI GGGWCCC 1 cut(s) 236
KspI CCGCGG 1 cut(s) 588
LguI GCTCTTC 1 cut(s) 1912
LmnI GCTCC 6 cut(s) 157, 367, 709, 790, 1889, 2655
LweI GCATC 9 cut(s) 413, 954, 1208, 1351, 1638, 1883, 2190, 2201, 3037
MabI ACCWGGT 1 cut(s) 649
MaeI CTAG 6 cut(s) 87, 822, 1902, 2439, 2774, 2862
MaeII ACGT 7 cut(s) 1060, 1115, 1429, 1627, 2260, 2871, 3177
MboII GAAGA 8 cut(s) 416, 1447, 1532, 1929, 2607, 2736, 2885, 3161
MfeI CAATTG 4 cut(s) 384, 393, 1739, 1995
MflI RGATCY 2 cut(s) 1102, 3112
MhlI GDGCHC 4 cut(s) 154, 1076, 1519, 1633
MlsI TGGCCA 3 cut(s) 300, 763, 2397
MluNI TGGCCA 3 cut(s) 300, 763, 2397
Mly113I GGCGCC 1 cut(s) 3067
MlyI GAGTC 3 cut(s) 1306, 1592, 3166
MmeI TCCRAC 4 cut(s) 532, 1725, 1781, 1987
Mox20I TGGCCA 3 cut(s) 300, 763, 2397
MroXI GAANNNNTTC 1 cut(s) 1752
MscI TGGCCA 3 cut(s) 300, 763, 2397
MseI TTAA 8 cut(s) 51, 1109, 1830, 1982, 2321, 2675, 2918, 3092
MslI CAYNNNNRTG 4 cut(s) 757, 1167, 2387, 2441
Msp20I TGGCCA 3 cut(s) 300, 763, 2397
MspA1I CMGCKG 6 cut(s) 587, 1445, 1502, 1937, 1970, 2126
MunI CAATTG 4 cut(s) 384, 393, 1739, 1995
Mva1269I GAATGC 3 cut(s) 840, 1128, 1483
MvaI CCWGG 6 cut(s) 333, 420, 651, 804, 1268, 2219
MvnI CGCG 4 cut(s) 229, 587, 1318, 2463
NarI GGCGCC 1 cut(s) 3067
NciI CCSGG 8 cut(s) 148, 183, 194, 286, 453, 504, 2286, 2287
NcoI CCATGG 1 cut(s) 2242
NmeAIII GCCGAG 2 cut(s) 157, 922
NmuCI GTSAC 7 cut(s) 1319, 2170, 2297, 2314, 2528, 2554, 2906
NspI RCATGY 5 cut(s) 436, 721, 2493, 2543, 3197
OliI CACNNNNGTG 1 cut(s) 2387
PaeI GCATGC 2 cut(s) 436, 721
PaeR7I CTCGAG 1 cut(s) 2897
PagI TCATGA 4 cut(s) 973, 1251, 2496, 2800
PaqCI CACCTGC 2 cut(s) 769, 889
PceI AGGCCT 2 cut(s) 1375, 2179
PciSI GCTCTTC 1 cut(s) 1912
PcsI WCGNNNNNNNCGW 3 cut(s) 1222, 2407, 2877
PctI GAATGC 3 cut(s) 840, 1128, 1483
PdmI GAANNNNTTC 1 cut(s) 1752
PfeI GAWTC 8 cut(s) 46, 1000, 1787, 2810, 2831, 2851, 2942, 2970
PflMI CCANNNNNTGG 4 cut(s) 386, 485, 551, 1189
Ple19I CGATCG 1 cut(s) 233
PleI GAGTC 3 cut(s) 1306, 1592, 3165
PluTI GGCGCC 1 cut(s) 3070
PmaCI CACGTG 1 cut(s) 2872
PmlI CACGTG 1 cut(s) 2872
PpsI GAGTC 3 cut(s) 1306, 1592, 3165
Ppu21I YACGTR 2 cut(s) 1430, 2872
PpuMI RGGWCCY 4 cut(s) 236, 410, 653, 1565
PshAI GACNNNNGTC 1 cut(s) 3156
PshBI ATTAAT 1 cut(s) 2675
Psp5II RGGWCCY 4 cut(s) 236, 410, 653, 1565
Psp6I CCWGG 6 cut(s) 331, 418, 649, 802, 1266, 2217
PspCI CACGTG 1 cut(s) 2872
PspEI GGTNACC 1 cut(s) 2314
PspFI CCCAGC 3 cut(s) 259, 622, 908
PspGI CCWGG 6 cut(s) 331, 418, 649, 802, 1266, 2217
PspPPI RGGWCCY 4 cut(s) 236, 410, 653, 1565
PstI CTGCAG 3 cut(s) 1102, 1507, 1638
PsuI RGATCY 2 cut(s) 1102, 3112
PvuI CGATCG 1 cut(s) 233
PvuII CAGCTG 5 cut(s) 1445, 1502, 1937, 1970, 2126
RsaI GTAC 8 cut(s) 898, 1428, 1451, 1837, 2240, 2544, 2769, 2780
RsaNI GTAC 8 cut(s) 897, 1427, 1450, 1836, 2239, 2543, 2768, 2779
RseI CAYNNNNRTG 4 cut(s) 757, 1167, 2387, 2441
SacII CCGCGG 1 cut(s) 588
SapI GCTCTTC 1 cut(s) 1912
SaqAI TTAA 8 cut(s) 51, 1109, 1830, 1982, 2321, 2675, 2918, 3092
ScaI AGTACT 1 cut(s) 1837
SchI GAGTC 3 cut(s) 1306, 1592, 3166
SduI GDGCHC 4 cut(s) 154, 1076, 1519, 1633
SexAI ACCWGGT 1 cut(s) 649
SfaNI GCATC 9 cut(s) 413, 954, 1208, 1351, 1638, 1883, 2190, 2201, 3037
SfcI CTRYAG 4 cut(s) 1098, 1503, 1634, 3008
SfoI GGCGCC 1 cut(s) 3068
Sfr274I CTCGAG 1 cut(s) 2897
Sfr303I CCGCGG 1 cut(s) 588
SgrBI CCGCGG 1 cut(s) 588
SlaI CTCGAG 1 cut(s) 2897
SmaI CCCGGG 1 cut(s) 2287
SmiMI CAYNNNNRTG 4 cut(s) 757, 1167, 2387, 2441
SmlI CTYRAG 2 cut(s) 2181, 2897
SmoI CTYRAG 2 cut(s) 2181, 2897
SpeI ACTAGT 2 cut(s) 2438, 2773
SphI GCATGC 2 cut(s) 436, 721
SseBI AGGCCT 2 cut(s) 1375, 2179
SspDI GGCGCC 1 cut(s) 3066
SspMI CTAG 6 cut(s) 87, 822, 1902, 2439, 2774, 2862
StuI AGGCCT 2 cut(s) 1375, 2179
StyI CCWWGG 1 cut(s) 2242
TaiI ACGT 7 cut(s) 1063, 1118, 1432, 1630, 2263, 2874, 3180
TaqI TCGA 6 cut(s) 322, 962, 1216, 2410, 2898, 3147
TatI WGTACW 2 cut(s) 1449, 1835
TauI GCSGC 8 cut(s) 61, 292, 439, 587, 724, 1725, 1805, 2510
TfiI GAWTC 8 cut(s) 46, 1000, 1787, 2810, 2831, 2851, 2942, 2970
Tru1I TTAA 8 cut(s) 51, 1109, 1830, 1982, 2321, 2675, 2918, 3092
Tru9I TTAA 8 cut(s) 51, 1109, 1830, 1982, 2321, 2675, 2918, 3092
TseFI GTSAC 7 cut(s) 1319, 2170, 2297, 2314, 2528, 2554, 2906
Tsp45I GTSAC 7 cut(s) 1319, 2170, 2297, 2314, 2528, 2554, 2906
TspGWI ACGGA 4 cut(s) 607, 1533, 2285, 2416
TspMI CCCGGG 1 cut(s) 2285
Van91I CCANNNNNTGG 4 cut(s) 386, 485, 551, 1189
VneI GTGCAC 3 cut(s) 1072, 1515, 1629
VspI ATTAAT 1 cut(s) 2675
XagI CCTNNNNNAGG 1 cut(s) 417
XceI RCATGY 5 cut(s) 436, 721, 2493, 2543, 3197
XcmI CCANNNNNNNNNTGG 2 cut(s) 1334, 3161
XhoI CTCGAG 1 cut(s) 2897
XmaI CCCGGG 1 cut(s) 2285
XmiI GTMKAC 1 cut(s) 1351
XmnI GAANNNNTTC 1 cut(s) 1752
XspI CTAG 6 cut(s) 87, 822, 1902, 2439, 2774, 2862
ZraI GACGTC 1 cut(s) 1116
ZrmI AGTACT 1 cut(s) 1837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.