MD15G1281700.v1.1

Protein transport protein Sec24-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000554
Physical Location & Seq
Forward (+)
25193639 .. 25207320
13682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1281700.v1.1.491

Sequence Viewer

Length: 3147 bp
ATGGCGGCTCTTGTGCGTCCCCCAGGGGCACCTAGGCCCAATAACAGTAACACTAACACCCCACCAGCCTCTAATTACAATCCTAACAATGCTCAGAGGAACCCTGATTCGTTAGCGGATAATATGCAGAATTTGAATCTTAATAGGCAGCCTTCCATGCCTAATTCTGCCCCTAGACCATCCCCTTTTGGCCAAGCACCAACTTTTCATTCATCAGCCCCTCCTGCTGGGGCTCCTGTTGCACCTCCCCCTTTTTCATTGCCTGGTCGTCCCCCAGCTGCACTTGCAAGACCTACAGCACCCCAATCAGGTCCCCCGCAACCTACATTATTCCCTGTCACCACCCCAGCAAGGCCAACTGGGCCGCCTGTTGGCCAGCCTTCGTCTTTTGTGTCTAGACTGCCTCCGGGATCGTTTCCCCCTTTGGGCGGTGTAGCTCCAGCATCTGGTCCTTTTCAGACGTCAGGTTTACCGAGTGGTCCAGTTTCAGTACCACCACCAGCATCAGATCCTCGCCCAGGTCCTGGTTCTTTTCCTTTGGGTAGCGGTCAGAGCATGCTCCCTACGACTGCACCAGGTAGATTGATGAATAATGGGCCACCGGTGTTTGGTTCTGGAGCTATGCTGAGCGGGCCCTGTTACCCTACCAGTGGAAATGTACCTCAACCACCTTTTGGACATCCACCAGCAGTGGCAACGGCAACAGGTCCTCCTCGAACACCAACCATGCACTCTATGCTGGGCAGTCCAGCAGTTACTGCTCCACCAGGTCCTGTTCAGCAGGCACCACCATTTTCAGCAGCAGCACCACCAATTTCAGTGGCACCACCTTTTTCAGTGGCACCACCATTTTCTGCAGCACCTCCAAGCATGCAGGCACCTCCGGGTGGCTCCACCTTCCCAATTTCCTGGTTATGGTCAATCACCCAGAATGTTTGGAATGCCACCCACACCACTACCAAATCAGTCCATGGCTACTATCTCACCTGCCGTTTGTCAAACTGGATCTCCTTTGACTGGGTCATCGAAGATAGATCCCAATCAAATTCCTCTGCCTGTACCAGGTTCCCTGCTACAAGTGATTACATTGTCAGAGACAATGGGAATTGCAGTCCACGTTACATGAGGTGCACCATCAATCAGATACCATGTACGGCTGATCTTTTGACAACATCAGGGATGCTGTTAGCTTTGTTGGTTGAACCATTTGCTCTTCCTCATCCAGATGAAGAACCAATCCAAGTTGTGGATTTTGGTGAAAGTGGTCCTGTTCGATGCTCTTGTTGCAAAGGCTACATTAATCCTTTCATGAAGTTTGTTGACCAGGGCTGGAAATTCATTTGTAACCTGTGTGGATTTACTGATGAGACTCCCCGTGACTACCACTGCAATCTTGGGCCAGATGGTAGACTTAGAGATGCTGATGAAAGACCTGAGCTATGTAGAGGAACAGTTGAATTTGTTGCTTCAAAAGAATACATGGTGCGTGATCCCATGCCAGCTGTGTACTTCTTTCTCATAGATGTGTCCATGAATGCCATTCAAACTGGTGCAACTGGTGCAGCTTGCAGTGCAATCAGTCAAGTTATTGCTGATCTTCCTCCTTTGATGCTTATTGTTCCTGATGTACAAGATGTTTATACTCCTCTGGAGACTGATGTAATTGTTCAGCTTTCAGAGTGCCATCAAAATTTGGAACAATTGTTGGAAAGTATTCCCACCATGTTTCAAAACAGTAAAACTGCTGAATCAGCCCTTGGTGCGGCAATCAAGGCTGCTTTCTTGGCAATGAAGAGTACTGGAGGGAAACTTTTGGTGTTTCAGTCAGTGCTGGCATCAACTGGAATTGGAGCCCTCTCTGCTAGAGAGGCCGAAGGAAGAGCTAATATATCTTCAGCTGAGAAGGAGGCCCATAAATTACTCCAACCAGCAGACAAAACATTAAAGACAATGGCAATTGAATTAGCTGAATTTCAGGTGTGTGTCAACTTATTTATCACTACCCAGTCATACATAGACATTGCTTCTATCTCCGTCATCCCAAGAACAACTGGAGGGCAGGTTTACTATTATTACCCCTTCTCAGCTGTCTCTGATCCTGCAAAGCTCTACAATGATCTTAGATGGAATGTCACAAGACCTCAAGGTCTTGAGGGGGTGATGCGTGTAAGATGTAGCCAGGGTATTCAAGTTCAAGAGTACCATGGGAGCTTTTGCAAACGTATCCCTACAGATGTTGACGTACTTGGGATTGACTGCGATAAAACTGTTATGGTGACCTTAAAACATGATGATAAATTACAGGATGGTTCAGAATGTGGTTTCCTGTGTGCCCTTTTATACACAACTGTGTATGGTCAACGGAGAATTCAAGTTACAACTTTGTCTCTGCCATGCACGAGTATGCTAAGTAATCTGTTTCATGCTGCTGATTTGGACACTCAGTTTGCGTGTTTCATGAAGCAAGCGGCAAACGAAATTCCTTCAAGTGCTCTCTTGCGTGTACGGGACCAAGTGACAAATCTGTGCATCAGTAGTCTGCTTTCATATTGTAAATTTTGTGCTACTGTATCATCTTCTGGACAGCTTATTCTTCCGGAGGCACTGAAGCTCCTGCCTCTATACACCCTTGCATTAATCAAAAGTATGGGGCTACGAAGTGATGGAAAAATAGATGAGCGGTCCTTCTGGATCAATCATGTATCTTCCCTTTCTGTTCCTTTGGCAGTTCCAATGGAGGGTGATGAATCTCTAATTCCTCCGGTGATTCCACTATCTAGCGAACACGTGAGTGACAAGGGAATTTATCTTCTAGAGAATGGTGAGGACTGTTTAATATATATTGGAAACTTGGTGGATCCTGGAATTTTGCAGCAACTGTTTGGAATCACCTCTGCTGATGGACTTCCTACTCAGTTTGTGCTGCAGCAGTATGATAATCCGTTATTGAAGAAGCTGAATGGTGTGGTAAATGAAATACGGCACCAAAGATGTTCCTACCTTCGCTTAAAGTTGTGCAAGAAAGGAGATCCGTCAGGAGCATTGTTTTTCTCATGCATGGTCGAAGACCAGAGTCCGAATGGCCCCTCCTATGTTGAGTTTCTGGTACATGTCCATCGGCAAATACAGATGAAAATGGCGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

1049

Amino Acids

112.58

Weight (kDa)

6.13

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 423 - 461 9.5e-14 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 498 - 535 4.8e-08 Sec23/Sec24 trunk domain
Sec23_trunk PF04811 535 - 711 9.4e-50 Sec23/Sec24 trunk domain
Sec23_BS PF08033 716 - 800 1.5e-20 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 811 - 909 3.1e-23 Sec23/Sec24 helical domain
Gelsolin PF00626 920 - 980 7.9e-06 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000554)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT4G32640 AT4G32640
fragaria_vesca FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000
malus_domestica MD02G1169200.v1.1 MD13G1230500.v1.1 MD15G1281700.v1.1 MD16G1009900.v1.1 MD16G1235300.v1.1
prunus_persica Prupe.1G056500_v2.0.a1 Prupe.1G056500_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1
pyrus_communis pycom02g13590 pycom13g20290 pycom15g24480 pycom16g00970 pycom16g19630
rosa_chinensis RchiOBHm_Chr4g0396591 RchiOBHm_Chr4g0445631
rosa_laevigata RLG00000005723 RLG00000009518
rosa_multiflora Rmu_co8031254.1_g000001 Rmu_co8351119.1_g000001 Rmu_sc0003637.1_g000024 Rmu_sc0010969.1_g000001 Rmu_sc0019383.1_g000002
rosa_roxburghii Rroxscaffold_5G00341440 Rroxscaffold_5G00386110
rosa_rugosa Rorug03G0348600 Rorug03G0348700 Rorug04G0363200 Rorug04G0363300 Rorug04G0363400 Rorug04G0363500
rosa_samantha Rh4AG070600 Rh4AG424700 Rh4AG425100 Rh4BG068500 Rh4BG436900 Rh4CG076300 Rh4CG451400 Rh4DG065600 Rh4DG433400
rosa_wichuraiana Rw4G005630 Rw4G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 995
AasI GACNNNNNNGTC 1 cut(s) 2145
AatII GACGTC 1 cut(s) 464
Acc36I ACCTGC 2 cut(s) 995, 2050
AccB1I GGYRCC 6 cut(s) 28, 784, 823, 841, 877, 2982
AccB7I CCANNNNNTGG 4 cut(s) 446, 524, 674, 1246
AccBSI CCGCTC 2 cut(s) 630, 2680
AccI GTMKAC 1 cut(s) 1408
AccIII TCCGGA 1 cut(s) 2596
AcoI YGGCCR 2 cut(s) 190, 373
AcuI CTGAAG 2 cut(s) 1878, 2627
AcvI CACGTG 1 cut(s) 2788
AcyI GRCGYC 2 cut(s) 461, 3140
AflIII ACRYGT 2 cut(s) 2785, 3109
AgeI ACCGGT 1 cut(s) 601
AjuI GAANNNNNNNTTGG 4 cut(s) 193, 225, 1740, 1772
AleI CACNNNNGTG 2 cut(s) 2348, 2790
Alw21I GWGCWC 2 cut(s) 1133, 2494
Alw26I GTCTC 5 cut(s) 1089, 1361, 1646, 2095, 2391
Alw44I GTGCAC 1 cut(s) 1129
AlwNI CAGNNNCTG 5 cut(s) 446, 524, 758, 773, 2878
Aor13HI TCCGGA 1 cut(s) 2596
ApaI GGGCCC 1 cut(s) 636
ApaLI GTGCAC 1 cut(s) 1129
ArsI GACNNNNNNTTYG 2 cut(s) 2429, 2461
AseI ATTAAT 2 cut(s) 1299, 2636
AsiGI ACCGGT 1 cut(s) 601
Asp700I GAANNNNTTC 1 cut(s) 1713
AspA2I CCTAGG 1 cut(s) 32
AsuC2I CCSGG 2 cut(s) 408, 885
AvaII GGWCC 9 cut(s) 311, 449, 479, 521, 707, 770, 1265, 2509, 2682
AvrII CCTAGG 1 cut(s) 32
BaeGI GKGCMC 4 cut(s) 31, 636, 1133, 2335
BalI TGGCCA 2 cut(s) 192, 375
BamHI GGATCC 1 cut(s) 2857
BanI GGYRCC 6 cut(s) 28, 784, 823, 841, 877, 2982
BanII GRGCYC 3 cut(s) 235, 636, 1855
BauI CACGAG 1 cut(s) 2398
BbrPI CACGTG 1 cut(s) 2788
BbsI GAAGAC 1 cut(s) 3072
Bbv12I GWGCWC 2 cut(s) 1133, 2494
BccI CCATC 9 cut(s) 187, 1142, 1397, 1692, 2118, 2300, 2657, 2894, 3123
BceAI ACGGC 4 cut(s) 714, 975, 1170, 2996
BciVI GTATCC 1 cut(s) 2234
BcnI CCSGG 2 cut(s) 408, 885
BcoDI GTCTC 5 cut(s) 1089, 1361, 1646, 2095, 2391
BfaI CTAG 6 cut(s) 33, 174, 396, 1863, 2778, 2813
BfmI CTRYAG 4 cut(s) 294, 855, 2229, 2924
BfuAI ACCTGC 2 cut(s) 995, 2050
BfuI GTATCC 1 cut(s) 2234
BglI GCCNNNNNGGC 1 cut(s) 361
BlnI CCTAGG 1 cut(s) 32
BlpI GCTNAGC 1 cut(s) 626
BmcAI AGTACT 1 cut(s) 1798
Bme18I GGWCC 9 cut(s) 311, 449, 479, 521, 707, 770, 1265, 2509, 2682
BmrI ACTGGG 3 cut(s) 369, 1027, 1999
BmsI GCATC 9 cut(s) 452, 512, 1170, 1265, 1408, 1599, 1844, 2151, 2538
BmuI ACTGGG 3 cut(s) 369, 1027, 1999
BoxI GACNNNNGTC 1 cut(s) 3072
BpiI GAAGAC 1 cut(s) 3072
BpmI CTGGAG 5 cut(s) 423, 636, 1670, 1821, 2073
Bpu10I CCTNAGC 1 cut(s) 1434
Bpu1102I GCTNAGC 1 cut(s) 626
BpuEI CTTGAG 2 cut(s) 2127, 2171
BpuMI CCSGG 2 cut(s) 408, 885
BsaAI YACGTR 1 cut(s) 2788
BsaBI GATNNNNATC 1 cut(s) 1039
BsaHI GRCGYC 2 cut(s) 461, 3140
BsaJI CCNNGG 9 cut(s) 22, 23, 32, 517, 970, 1324, 1756, 2179, 2203
BsaWI WCCGGW 3 cut(s) 601, 2596, 2761
BsaXI ACNNNNNCTCC 8 cut(s) 694, 724, 992, 1022, 2046, 2076, 2595, 2625
Bse118I RCCGGY 1 cut(s) 601
Bse3DI GCAATG 3 cut(s) 257, 1794, 2019
Bse8I GATNNNNATC 1 cut(s) 1039
BseAI TCCGGA 1 cut(s) 2596
BseDI CCNNGG 9 cut(s) 22, 23, 32, 517, 970, 1324, 1756, 2179, 2203
BseGI GGATG 6 cut(s) 179, 679, 1185, 1219, 2037, 2311
BseJI GATNNNNATC 1 cut(s) 1039
BseMI GCAATG 3 cut(s) 257, 1794, 2019
BseMII CTCAG 7 cut(s) 107, 617, 1425, 1890, 2097, 2456, 2927
BseRI GAGGAG 2 cut(s) 702, 1635
BseSI GKGCMC 4 cut(s) 31, 636, 1133, 2335
BseYI CCCAGC 4 cut(s) 227, 274, 346, 739
BsgI GTGCAG 3 cut(s) 264, 555, 1581
BshNI GGYRCC 6 cut(s) 28, 784, 823, 841, 877, 2982
BshTI ACCGGT 1 cut(s) 601
BsiHKAI GWGCWC 2 cut(s) 1133, 2494
BsiSI CCGG 5 cut(s) 407, 602, 884, 2597, 2762
BslFI GGGAC 4 cut(s) 3, 255, 297, 2522
BsmAI GTCTC 5 cut(s) 1089, 1361, 1646, 2095, 2391
BsmFI GGGAC 4 cut(s) 3, 255, 297, 2522
BsmI GAATGC 2 cut(s) 946, 1540
Bsp120I GGGCCC 1 cut(s) 632
Bsp1286I GDGCHC 7 cut(s) 31, 235, 636, 1133, 1855, 2335, 2494
Bsp13I TCCGGA 1 cut(s) 2596
Bsp1407I TGTACA 1 cut(s) 1627
Bsp1720I GCTNAGC 1 cut(s) 626
Bsp19I CCATGG 2 cut(s) 970, 2203
BspCNI CTCAG 7 cut(s) 106, 618, 1426, 1891, 2096, 2455, 2926
BspEI TCCGGA 1 cut(s) 2596
BspHI TCATGA 2 cut(s) 1308, 2457
BspMAI CTGCAG 2 cut(s) 859, 2928
BspMI ACCTGC 2 cut(s) 995, 2050
BspQI GCTCTTC 2 cut(s) 1218, 1873
BspT107I GGYRCC 6 cut(s) 28, 784, 823, 841, 877, 2982
BsrBI CCGCTC 2 cut(s) 630, 2680
BsrDI GCAATG 3 cut(s) 257, 1794, 2019
BsrFI RCCGGY 1 cut(s) 601
BsrGI TGTACA 1 cut(s) 1627
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 9 cut(s) 22, 23, 32, 517, 970, 1324, 1756, 2179, 2203
BssNI GRCGYC 2 cut(s) 461, 3140
BssSI CACGAG 1 cut(s) 2398
BssT1I CCWWGG 4 cut(s) 32, 970, 1756, 2203
Bst2BI CACGAG 1 cut(s) 2398
Bst4CI ACNGT 8 cut(s) 47, 1453, 1736, 2269, 2350, 2569, 2831, 2880
Bst6I CTCTTC 3 cut(s) 1218, 1787, 1873
BstACI GRCGYC 2 cut(s) 461, 3140
BstAPI GCANNNNNTGC 3 cut(s) 736, 758, 1559
BstAUI TGTACA 1 cut(s) 1627
BstBAI YACGTR 1 cut(s) 2788
BstDSI CCRYGG 2 cut(s) 970, 2203
BstEII GGTNACC 1 cut(s) 2275
BstENI CCTNNNNNAGG 2 cut(s) 516, 1060
BstF5I GGATG 6 cut(s) 179, 679, 1185, 1219, 2037, 2311
BstMAI GTCTC 5 cut(s) 1089, 1361, 1646, 2095, 2391
BstNSI RCATGY 3 cut(s) 559, 874, 3113
BstPAI GACNNNNGTC 1 cut(s) 3072
BstPI GGTNACC 1 cut(s) 2275
BstSFI CTRYAG 4 cut(s) 294, 855, 2229, 2924
BstSLI GKGCMC 4 cut(s) 31, 636, 1133, 2335
BstV2I GAAGAC 1 cut(s) 3072
BstX2I RGATCY 5 cut(s) 508, 1005, 1034, 2857, 3028
BstXI CCANNNNNNTGG 1 cut(s) 909
BstYI RGATCY 5 cut(s) 508, 1005, 1034, 2857, 3028
BsuI GTATCC 1 cut(s) 2234
BtgI CCRYGG 2 cut(s) 970, 2203
BtsCI GGATG 6 cut(s) 179, 679, 1185, 1219, 2037, 2311
BtsI GCAGTG 3 cut(s) 696, 1384, 1576
BtsIMutI CAGTG 8 cut(s) 655, 696, 825, 843, 1384, 1576, 1833, 2603
BveI ACCTGC 2 cut(s) 995, 2050
CaiI CAGNNNCTG 5 cut(s) 446, 524, 758, 773, 2878
CciI TCATGA 2 cut(s) 1308, 2457
Cfr10I RCCGGY 1 cut(s) 601
CseI GACGC 2 cut(s) 5, 3129
CsiI ACCWGGT 3 cut(s) 574, 766, 1061
CspAI ACCGGT 1 cut(s) 601
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
DrdI GACNNNNNNGTC 1 cut(s) 2145
DseDI GACNNNNNNGTC 1 cut(s) 2145
EaeI YGGCCR 2 cut(s) 190, 373
Eam1104I CTCTTC 3 cut(s) 1218, 1787, 1873
EarI CTCTTC 3 cut(s) 1218, 1787, 1873
Eco130I CCWWGG 4 cut(s) 32, 970, 1756, 2203
Eco24I GRGCYC 3 cut(s) 235, 636, 1855
Eco47I GGWCC 9 cut(s) 311, 449, 479, 521, 707, 770, 1265, 2509, 2682
Eco57I CTGAAG 2 cut(s) 1878, 2627
Eco72I CACGTG 1 cut(s) 2788
Eco91I GGTNACC 1 cut(s) 2275
EcoNI CCTNNNNNAGG 2 cut(s) 516, 1060
EcoO109I RGGNCCY 5 cut(s) 311, 521, 633, 707, 770
EcoO65I GGTNACC 1 cut(s) 2275
EcoRI GAATTC 1 cut(s) 2367
EcoT14I CCWWGG 4 cut(s) 32, 970, 1756, 2203
EcoT22I ATGCAT 1 cut(s) 3059
EcoT38I GRGCYC 3 cut(s) 235, 636, 1855
ErhI CCWWGG 4 cut(s) 32, 970, 1756, 2203
FaqI GGGAC 4 cut(s) 3, 255, 297, 2522
FauI CCCGC 2 cut(s) 324, 623
FblI GTMKAC 1 cut(s) 1408
FokI GGATG 6 cut(s) 166, 666, 1192, 1206, 2024, 2318
FriOI GRGCYC 3 cut(s) 235, 636, 1855
FspBI CTAG 6 cut(s) 33, 174, 396, 1863, 2778, 2813
GsaI CCCAGC 4 cut(s) 231, 278, 350, 743
GsuI CTGGAG 5 cut(s) 423, 636, 1670, 1821, 2073
HapII CCGG 5 cut(s) 407, 602, 884, 2597, 2762
HgaI GACGC 2 cut(s) 5, 3129
Hin1I GRCGYC 2 cut(s) 461, 3140
HincII GTYRAC 4 cut(s) 1321, 1987, 2239, 2360
HindII GTYRAC 4 cut(s) 1321, 1987, 2239, 2360
HinfI GANTC 8 cut(s) 107, 136, 1369, 1748, 2747, 2767, 2886, 3073
HpaII CCGG 5 cut(s) 407, 602, 884, 2597, 2762
HpyAV CCTTC 9 cut(s) 162, 390, 907, 1868, 1897, 2089, 2493, 2695, 3011
HpyCH4III ACNGT 8 cut(s) 47, 1453, 1736, 2269, 2350, 2569, 2831, 2880
HpyCH4IV ACGT 5 cut(s) 461, 1117, 2221, 2241, 2787
HpySE526I ACGT 5 cut(s) 461, 1117, 2221, 2241, 2787
Hsp92I GRCGYC 2 cut(s) 461, 3140
Kpn2I TCCGGA 1 cut(s) 2596
LguI GCTCTTC 2 cut(s) 1218, 1873
LweI GCATC 9 cut(s) 452, 512, 1170, 1265, 1408, 1599, 1844, 2151, 2538
MabI ACCWGGT 3 cut(s) 574, 766, 1061
MaeI CTAG 6 cut(s) 33, 174, 396, 1863, 2778, 2813
MaeII ACGT 5 cut(s) 461, 1117, 2221, 2241, 2787
MbiI CCGCTC 2 cut(s) 630, 2680
MfeI CAATTG 2 cut(s) 1700, 1956
MflI RGATCY 5 cut(s) 508, 1005, 1034, 2857, 3028
MhlI GDGCHC 7 cut(s) 31, 235, 636, 1133, 1855, 2335, 2494
MlsI TGGCCA 2 cut(s) 192, 375
MluNI TGGCCA 2 cut(s) 192, 375
MlyI GAGTC 2 cut(s) 1363, 3082
MmeI TCCRAC 2 cut(s) 1686, 1948
Mox20I TGGCCA 2 cut(s) 192, 375
Mph1103I ATGCAT 1 cut(s) 3059
MroI TCCGGA 1 cut(s) 2596
MroXI GAANNNNTTC 1 cut(s) 1713
MscI TGGCCA 2 cut(s) 192, 375
MseI TTAA 7 cut(s) 141, 1299, 1943, 2282, 2636, 2834, 3008
MslI CAYNNNNRTG 5 cut(s) 1224, 1523, 2348, 2402, 2790
Msp20I TGGCCA 2 cut(s) 192, 375
MspA1I CMGCKG 4 cut(s) 278, 1502, 1898, 2087
MspI CCGG 5 cut(s) 407, 602, 884, 2597, 2762
MunI CAATTG 2 cut(s) 1700, 1956
Mva1269I GAATGC 2 cut(s) 946, 1540
NciI CCSGG 2 cut(s) 408, 885
NcoI CCATGG 2 cut(s) 970, 2203
NmuCI GTSAC 6 cut(s) 337, 1376, 2131, 2275, 2515, 2792
NsiI ATGCAT 1 cut(s) 3059
NspI RCATGY 3 cut(s) 559, 874, 3113
OliI CACNNNNGTG 2 cut(s) 2348, 2790
PaeI GCATGC 2 cut(s) 559, 874
PagI TCATGA 2 cut(s) 1308, 2457
PaqCI CACCTGC 1 cut(s) 995
PasI CCCWGGG 1 cut(s) 23
PciI ACATGT 1 cut(s) 3109
PciSI GCTCTTC 2 cut(s) 1218, 1873
PctI GAATGC 2 cut(s) 946, 1540
PdmI GAANNNNTTC 1 cut(s) 1713
PfeI GAWTC 6 cut(s) 107, 136, 1748, 2747, 2767, 2886
PflFI GACNNNGTC 1 cut(s) 1019
PflMI CCANNNNNTGG 4 cut(s) 446, 524, 674, 1246
PfoI TCCNGGA 2 cut(s) 406, 2860
PinAI ACCGGT 1 cut(s) 601
PleI GAGTC 2 cut(s) 1363, 3081
PmaCI CACGTG 1 cut(s) 2788
PmlI CACGTG 1 cut(s) 2788
PpsI GAGTC 2 cut(s) 1363, 3081
Ppu21I YACGTR 1 cut(s) 2788
PpuMI RGGWCCY 4 cut(s) 311, 521, 707, 770
PscI ACATGT 1 cut(s) 3109
PshAI GACNNNNGTC 1 cut(s) 3072
PshBI ATTAAT 2 cut(s) 1299, 2636
Psp5II RGGWCCY 4 cut(s) 311, 521, 707, 770
PspCI CACGTG 1 cut(s) 2788
PspEI GGTNACC 1 cut(s) 2275
PspFI CCCAGC 4 cut(s) 227, 274, 346, 739
PspOMI GGGCCC 1 cut(s) 632
PspPPI RGGWCCY 4 cut(s) 311, 521, 707, 770
PstI CTGCAG 2 cut(s) 859, 2928
PstNI CAGNNNCTG 5 cut(s) 446, 524, 758, 773, 2878
PsuI RGATCY 5 cut(s) 508, 1005, 1034, 2857, 3028
PsyI GACNNNGTC 1 cut(s) 1019
PvuII CAGCTG 4 cut(s) 278, 1502, 1898, 2087
RseI CAYNNNNRTG 5 cut(s) 1224, 1523, 2348, 2402, 2790
SapI GCTCTTC 2 cut(s) 1218, 1873
SaqAI TTAA 7 cut(s) 141, 1299, 1943, 2282, 2636, 2834, 3008
ScaI AGTACT 1 cut(s) 1798
SchI GAGTC 2 cut(s) 1363, 3082
SduI GDGCHC 7 cut(s) 31, 235, 636, 1133, 1855, 2335, 2494
SexAI ACCWGGT 3 cut(s) 574, 766, 1061
SfaNI GCATC 9 cut(s) 452, 512, 1170, 1265, 1408, 1599, 1844, 2151, 2538
SfcI CTRYAG 4 cut(s) 294, 855, 2229, 2924
SfiI GGCCNNNNNGGCC 1 cut(s) 361
SgrAI CRCCGGYG 1 cut(s) 601
SinI GGWCC 9 cut(s) 311, 449, 479, 521, 707, 770, 1265, 2509, 2682
SmiMI CAYNNNNRTG 5 cut(s) 1224, 1523, 2348, 2402, 2790
SmlI CTYRAG 2 cut(s) 2142, 2150
SmoI CTYRAG 2 cut(s) 2142, 2150
SphI GCATGC 2 cut(s) 559, 874
SspMI CTAG 6 cut(s) 33, 174, 396, 1863, 2778, 2813
StyI CCWWGG 4 cut(s) 32, 970, 1756, 2203
TaaI ACNGT 8 cut(s) 47, 1453, 1736, 2269, 2350, 2569, 2831, 2880
TaiI ACGT 5 cut(s) 464, 1120, 2224, 2244, 2790
TaqI TCGA 4 cut(s) 715, 1026, 1273, 3063
TatI WGTACW 3 cut(s) 1506, 1627, 1796
TauI GCSGC 4 cut(s) 8, 367, 1766, 2471
TfiI GAWTC 6 cut(s) 107, 136, 1748, 2747, 2767, 2886
Tru1I TTAA 7 cut(s) 141, 1299, 1943, 2282, 2636, 2834, 3008
Tru9I TTAA 7 cut(s) 141, 1299, 1943, 2282, 2636, 2834, 3008
TscAI CASTG 8 cut(s) 655, 696, 825, 843, 1391, 1576, 1833, 2610
TseFI GTSAC 6 cut(s) 337, 1376, 2131, 2275, 2515, 2792
Tsp45I GTSAC 6 cut(s) 337, 1376, 2131, 2275, 2515, 2792
TspGWI ACGGA 4 cut(s) 2023, 2377, 2931, 3021
TspRI CASTG 8 cut(s) 655, 696, 825, 843, 1391, 1576, 1833, 2610
Tth111I GACNNNGTC 1 cut(s) 1019
Van91I CCANNNNNTGG 4 cut(s) 446, 524, 674, 1246
VneI GTGCAC 1 cut(s) 1129
VpaK11BI GGWCC 9 cut(s) 311, 449, 479, 521, 707, 770, 1265, 2509, 2682
VspI ATTAAT 2 cut(s) 1299, 2636
XagI CCTNNNNNAGG 2 cut(s) 516, 1060
XbaI TCTAGA 2 cut(s) 395, 2812
XceI RCATGY 3 cut(s) 559, 874, 3113
XcmI CCANNNNNNNNNTGG 2 cut(s) 1391, 3077
XmaJI CCTAGG 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 1408
XmnI GAANNNNTTC 1 cut(s) 1713
XspI CTAG 6 cut(s) 33, 174, 396, 1863, 2778, 2813
ZraI GACGTC 1 cut(s) 462
ZrmI AGTACT 1 cut(s) 1798
Zsp2I ATGCAT 1 cut(s) 3059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.