Rh4CG076300

Protein transport protein Sec24-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000554
Physical Location & Seq
Reverse (-)
13874388 .. 13888740
14353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG076300.1

Sequence Viewer

Length: 3408 bp
ATGGCAGCTTTTGTGCCTCCTCCAGGGGCACCTAGGCCCAATAACAGTAACACTCCACCAGGGGCACCGCCCCCAAATTACAACCCTAACAATGCTCAGAGAAACCCCGATTCGTTATCCGGTAACATGCAGAATTTGAATCTTAATCGCCAGCAGTCTCCTCCCATGCCCAATTCTGCTCCTAGACCATCATCATCATCATCATCATCATCATCATCATCCCCTTTTGGCCAACCGCCGCCCTTCCAGTCATCACCGCCTCCCCCGGGGGCACCTGGTGCTTCTGCGCCTTATTCTCGGCCTGGCCCTCCCCCGGCCAGACCTGCAGCACCCCGTCCACAAGCAACATTACCCCCTGGCACCGCCGCAGTAAGACCTACTGGCCCACCTGTTGGCCAGTCTTCACCGTTTGGGTCTAGACCGCCCCCTGGGGCTTTTCCTCCTGGGGTAGCTCCTACTTCTGGCCCAGCTTCCAGTCCTTTCCAGACAACGGGTTTGCCAAGTGGTCCAGGTTCAACGCCACCACCGCCTTCGTCAGGTCCTCGCCCTGGTCCTGGGCCGTTCGCTAGCGGTCAGGCTGTGCCCCCTATGGGCGCACCGGGTAGAATGAGTAATGGGCCACCGGTCTTTGGTTCTGGGGCTGTGCCAGGTGGACCTCGTTTTCCTCCAACTGGAAATTTACCTCAACCACCTGTTGGATCTTCACCAACTTCAGTGGCTCCTCCACGTGCTCCAACCATGCATTCGGTGCTCGGTGGTCCAGCAGTCGGTGCTCCACCAGGATCTACTGCCCAACAAGCACCGCCATTTTCGTCAGGTCCTCAAGGCATGCGGCCTCCTCCTCCTGGTTCTCCTTATAGCCCACAGTCATGGCCAATGCAACAAGGGGTGGCTCAACCTCCACAATTTCCTGGGTCTGCTCCACCTCCACAATTTCCTGGCTCTGCTCCACCCCCACAATTTCCTGGCTCTTCTCCACCCCCACAATTTCCTGGCTCTGCTCAACCACCTAGAATGTATGGAATGCCACCACAACCGCTGCCAAATCAGTCCATGACTACCATCTCATCGGCTGTTGGTCAAACTGGAACTCCTATGGCTGGGTCATCAAAGATTGATCCCAATCAAATTCCTCGGCCTGGACCAAGTTCTTCAGTACTTCTGCATGAAACTCGTCAGGCCAATCAAGCAAATCCACCTCCGCCTGCTACAAGTGATTATATCGTCAGAGACACTGGGAATTGCAGTCCACGTTACATGAGGTGCACCATCAATCAGATACCATGCACTGCTGATCTTTTGACAACATCAGGGATGCCGTTAGCTTTGTTGGTTGAACCTTTTGCCCTTCCTCATCCATCTGAGGAACCAATCCAAATTGTAGATTTTGGTGAAAGTGGCCCTGTTCGATGTTCTCGTTGCAAAGGCTACATAAATCCTTTCATGAGGTTCATAGACCAGGGCAGGCAATTCATCTGCAATTTGTGTGGATTTACTGATGAGACTCCCCGCGACTACCACTGCAATCTAGGTCCAGATGGCAGACGTAGAGATGCTGATGATCGGCCTGAGCTATGTAGGGGAACAGTTGAATTTGTTGCCTCAAAAGAATACATGGTGCGTGATCCCATGCCAGCTGTGTACTTCTTCCTTATCGATGTATCCATGAATGCCATTCAAACTGGTGCAACTGCTGCAGCTTGCAGCGCAATCAATCAAGTTATTGCTGATCTTCCTGAAGGTCCTCGGACAAGGGTGGGGATAGCGACATTTGACTCGACAATCCATTTTTACAATTTGAAACGTGCACTGCAGCAGCCTTTGATGCTTATTGTTCCTGATGTACAAGATGTTTACACTCCTCTAGAGACTGATGTAGTTGTTCAGCTTTCCGAGTGCCGTCAACATTTGGAGCAATTGCTGGAAAGTATTCCAACTATGTTTCAGAATAGTAAAACTGCTGAATCAGCTTTTGGTGCAGCAATCAAGGCTGCTTTCTTGGCAATAAAGAGTACTGGAGGGAAACTTTTGGTGTTTCAGTCAGTGTTGCCATCAACTGGAATTGGAGCGCTTTCAGCTAGAGAGGCTGAAGGAAGAGCTAATATATCTTCAGCTGAGAAGGAGCCCAATAAATTACTCCAGCCCGTGGACAAAACTTTAAAGACATTGGCAATTGAATTTGCTGAATATCAGGTTTGCGTTGATTTATTTATCACTACCCAGTCATACATAGATATTGCTTCTATCTCAGTCATCCCCAGAACAACCGGAGGGCAGGTTTACTATTATTATCCCTTCTCAGCTCTCTCTGATCCTGCAAAGCTATATAATGATCTTAGATGGAATGTCACAAGGCCGCAAGGTTTTGAGGCAGTGATGCGTGTAAGATGCAGCCAGGGTATTCAAGTTCAAGAGTACTTTGGAAACTTTTGCAAACGCATTCCAACAGATGTCGACCTACCTGGGATTGACTGTGACAAAACTATTATGGTAATGTTAAAGCATGATGACAAATTACAGGATGGCTCAGAATGTTCTTTCCAGTGTGCTGTTTTATACACAACTGTGTATGGCCAACGGAGAATTCGGGTTACCACTTTGTCTCTGCCATGCACTAGTATGCTAAGTAATCTGTTTCGCGCTGCTGATTTGGACACTCAGTTTTCATGTTACATGAAGCAAGCGGCAAATGAAATCACGTCAAGTCCCCTCTTGCGTGCACGGGAACAAATGACAAATCTTTGCATCAATAGTCTGCTTTCATACCGCAAATTCTGTGCTACTGTATCATCTTCGGGACAGCTTATTCTTCCAGAGGCACTGAAGCTCCTGCCTCTATACACCCTTGCATTAATCAAAAGTACCGGACTACGAACTGATGGAAAAATTGATGAGCGGTCTTTCTGGATCAACCATGTTTCCTCCCTGTCTGTACCTTTGGCAGTTCCACTGGTGTATCCCAGGATGGTGGCTGTCCATGACCTTGATGCTCAAAAGGAGGGTGATGGATCTCTTATTCCTCCGGTGATTCCACTTTCTAGCGAACACGTTTGTGATGATGGAATATATCTTCTTGAGAATGGTGAGGACTGTTTAATCTACGTTGGGAACTCGGTGGATTCTGGAATTTTACAACAGCTGTTTGGCGTCACCACTGCTGATGAACTTCCTACTCAGTTCGTGCTGCAGCAGTATGATAATTCATTATCAAAGAAGCTGAATGATGTGGTAAATGAAATACGCCGCCAGAGATGTTCCTACCTGCGATTGAAATTGTGCAAGAAAGGAGATCCTTCAGGAGCATTCTTCTTATCACATTTGGTTGAAGACCAGAGTCCCCATGGCCCCTCCTATGTTGAGTTTCTGGTACATGTCCATCGGCAAATACAGATAAAAATGACTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

1135

Amino Acids

121.4

Weight (kDa)

7.9

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 468 - 506 3.4e-16 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 543 - 783 4.1e-80 Sec23/Sec24 trunk domain
Sec23_BS PF08033 788 - 872 1.3e-27 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 883 - 981 6.2e-25 Sec23/Sec24 helical domain
Gelsolin PF00626 1007 - 1073 7.1e-08 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000554)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT3G44340 AT4G32640 AT4G32640
fragaria_vesca FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g05490 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000 FvH4_4g36000
malus_domestica MD02G1169200.v1.1 MD13G1230500.v1.1 MD15G1281700.v1.1 MD16G1009900.v1.1 MD16G1235300.v1.1
prunus_persica Prupe.1G056500_v2.0.a1 Prupe.1G056500_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1 Prupe.1G340700_v2.0.a1
pyrus_communis pycom02g13590 pycom13g20290 pycom15g24480 pycom16g00970 pycom16g19630
rosa_chinensis RchiOBHm_Chr4g0396591 RchiOBHm_Chr4g0445631
rosa_laevigata RLG00000005723 RLG00000009518
rosa_multiflora Rmu_co8031254.1_g000001 Rmu_co8351119.1_g000001 Rmu_sc0003637.1_g000024 Rmu_sc0010969.1_g000001 Rmu_sc0019383.1_g000002
rosa_roxburghii Rroxscaffold_5G00341440 Rroxscaffold_5G00386110
rosa_rugosa Rorug03G0348600 Rorug03G0348700 Rorug04G0363200 Rorug04G0363300 Rorug04G0363400 Rorug04G0363500
rosa_samantha Rh4AG070600 Rh4AG424700 Rh4AG425100 Rh4BG068500 Rh4BG436900 Rh4CG076300 Rh4CG451400 Rh4DG065600 Rh4DG433400
rosa_wichuraiana Rw4G005630 Rw4G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 331, 2266, 3270
AccB1I GGYRCC 4 cut(s) 28, 64, 271, 359
AccB7I CCANNNNNTGG 4 cut(s) 392, 695, 2057, 2146
AccBSI CCGCTC 1 cut(s) 2896
AccI GTMKAC 1 cut(s) 2454
AccII CGCG 2 cut(s) 1512, 2640
AclWI GGATC 8 cut(s) 706, 790, 1112, 1619, 2306, 2915, 3016, 3284
AcoI YGGCCR 5 cut(s) 229, 315, 394, 872, 2572
AcsI RAATTY 8 cut(s) 133, 676, 1128, 1592, 2177, 2583, 2771, 3126
AcuI CTGAAG 7 cut(s) 696, 1137, 1758, 2094, 2109, 2843, 3279
AcvI CACGTG 1 cut(s) 728
AcyI GRCGYC 1 cut(s) 3147
AdeI CACNNNGTG 1 cut(s) 278
AfaI GTAC 8 cut(s) 1158, 1643, 1845, 2014, 2417, 2863, 2934, 3369
AfeI AGCGCT 1 cut(s) 2070
AflIII ACRYGT 2 cut(s) 3046, 3370
AgeI ACCGGT 1 cut(s) 622
AhlI ACTAGT 1 cut(s) 2615
AjiI CACGTC 1 cut(s) 2700
AjuI GAANNNNNNNTTGG 6 cut(s) 727, 759, 1956, 1988, 2568, 2600
AleI CACNNNNGTG 2 cut(s) 2564, 3051
Alw21I GWGCWC 6 cut(s) 733, 753, 775, 1268, 1810, 2722
Alw26I GTCTC 5 cut(s) 162, 1224, 1496, 1862, 2607
Alw44I GTGCAC 3 cut(s) 1264, 1806, 2718
AlwI GGATC 8 cut(s) 706, 790, 1112, 1619, 2306, 2915, 3016, 3284
Ama87I CYCGRG 1 cut(s) 265
Aor51HI AGCGCT 1 cut(s) 2070
ApaLI GTGCAC 3 cut(s) 1264, 1806, 2718
ApoI RAATTY 8 cut(s) 133, 676, 1128, 1592, 2177, 2583, 2771, 3126
ArsI GACNNNNNNTTYG 2 cut(s) 478, 510
AseI ATTAAT 1 cut(s) 2852
AsiGI ACCGGT 1 cut(s) 622
Asp700I GAANNNNTTC 1 cut(s) 1929
AspA2I CCTAGG 1 cut(s) 32
AspLEI GCGC 5 cut(s) 289, 596, 1709, 2071, 2642
AsuC2I CCSGG 4 cut(s) 266, 267, 314, 600
AsuHPI GGTGA 8 cut(s) 246, 396, 696, 1403, 3014, 3037, 3095, 3142
AsuNHI GCTAGC 1 cut(s) 566
AvaI CYCGRG 1 cut(s) 265
AvaII GGWCC 9 cut(s) 506, 539, 551, 653, 758, 818, 1142, 1532, 1742
AvrII CCTAGG 1 cut(s) 32
BaeGI GKGCMC 7 cut(s) 31, 67, 274, 585, 1268, 1810, 2722
BaeI ACNNNNGTAYC 2 cut(s) 2939, 2972
BalI TGGCCA 4 cut(s) 231, 396, 874, 2574
BanI GGYRCC 4 cut(s) 28, 64, 271, 359
BanII GRGCYC 1 cut(s) 2127
BbrPI CACGTG 1 cut(s) 728
BbsI GAAGAC 2 cut(s) 393, 3333
Bbv12I GWGCWC 6 cut(s) 733, 753, 775, 1268, 1810, 2722
BceAI ACGGC 3 cut(s) 544, 1303, 1884
BcgI CGANNNNNNTGC 2 cut(s) 1154, 1188
BciVI GTATCC 2 cut(s) 1672, 2967
BcnI CCSGG 4 cut(s) 266, 267, 314, 600
BcoDI GTCTC 5 cut(s) 162, 1224, 1496, 1862, 2607
BcuI ACTAGT 1 cut(s) 2615
BfmI CTRYAG 4 cut(s) 324, 1695, 1811, 3185
BfoI RGCGCY 1 cut(s) 2072
BfuAI ACCTGC 3 cut(s) 331, 2266, 3270
BfuI GTATCC 2 cut(s) 1672, 2967
BlnI CCTAGG 1 cut(s) 32
BmcAI AGTACT 3 cut(s) 1158, 2014, 2417
Bme18I GGWCC 9 cut(s) 506, 539, 551, 653, 758, 818, 1142, 1532, 1742
BmeT110I CYCGRG 1 cut(s) 265
BmgBI CACGTC 1 cut(s) 2700
BmiI GGNNCC 8 cut(s) 30, 66, 273, 361, 720, 1368, 2124, 3346
BmrI ACTGGG 2 cut(s) 1245, 2215
BmsI GCATC 7 cut(s) 1305, 1543, 1815, 2367, 2378, 2754, 2977
BmtI GCTAGC 1 cut(s) 570
BmuI ACTGGG 2 cut(s) 1245, 2215
BoxI GACNNNNGTC 1 cut(s) 3333
BpiI GAAGAC 2 cut(s) 393, 3333
BpmI CTGGAG 3 cut(s) 6, 2037, 2123
Bpu10I CCTNAGC 1 cut(s) 1569
BpuEI CTTGAG 2 cut(s) 807, 3095
BpuMI CCSGG 4 cut(s) 266, 267, 314, 600
Bsa29I ATCGAT 1 cut(s) 1656
BsaAI YACGTR 1 cut(s) 728
BsaBI GATNNNNATC 1 cut(s) 1122
BsaHI GRCGYC 1 cut(s) 3147
BsaWI WCCGGW 5 cut(s) 119, 622, 2267, 2864, 3022
BsaXI ACNNNNNCTCC 6 cut(s) 1075, 1105, 2262, 2292, 2811, 2841
Bse118I RCCGGY 1 cut(s) 622
Bse8I GATNNNNATC 1 cut(s) 1122
BseCI ATCGAT 1 cut(s) 1656
BseGI GGATG 6 cut(s) 218, 1320, 1354, 2253, 2527, 2970
BseJI GATNNNNATC 1 cut(s) 1122
BseMII CTCAG 9 cut(s) 110, 1353, 1560, 2106, 2262, 2313, 2541, 2672, 3188
BseRI GAGGAG 6 cut(s) 9, 150, 711, 828, 831, 1851
BseSI GKGCMC 7 cut(s) 31, 67, 274, 585, 1268, 1810, 2722
BseYI CCCAGC 2 cut(s) 466, 1100
BsgI GTGCAG 1 cut(s) 1998
Bsh1236I CGCG 2 cut(s) 1512, 2640
BshNI GGYRCC 4 cut(s) 28, 64, 271, 359
BshTI ACCGGT 1 cut(s) 622
BshVI ATCGAT 1 cut(s) 1656
BsiHKAI GWGCWC 6 cut(s) 733, 753, 775, 1268, 1810, 2722
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 8 cut(s) 120, 266, 314, 599, 623, 2268, 2865, 3023
BslFI GGGAC 3 cut(s) 2691, 2811, 3321
BsmAI GTCTC 5 cut(s) 162, 1224, 1496, 1862, 2607
BsmFI GGGAC 3 cut(s) 2691, 2811, 3321
BsmI GAATGC 5 cut(s) 742, 1029, 1675, 2439, 3302
BsoBI CYCGRG 1 cut(s) 265
Bsp1407I TGTACA 1 cut(s) 1843
Bsp19I CCATGG 1 cut(s) 3340
BspCNI CTCAG 9 cut(s) 109, 1354, 1561, 2107, 2261, 2312, 2540, 2671, 3187
BspDI ATCGAT 1 cut(s) 1656
BspFNI CGCG 2 cut(s) 1512, 2640
BspHI TCATGA 1 cut(s) 1443
BspLI GGNNCC 8 cut(s) 30, 66, 273, 361, 720, 1368, 2124, 3346
BspMAI CTGCAG 4 cut(s) 328, 1699, 1815, 3189
BspMI ACCTGC 3 cut(s) 331, 2266, 3270
BspOI GCTAGC 1 cut(s) 570
BspPI GGATC 8 cut(s) 706, 790, 1112, 1619, 2306, 2915, 3016, 3284
BspQI GCTCTTC 2 cut(s) 976, 2089
BspT107I GGYRCC 4 cut(s) 28, 64, 271, 359
BsrBI CCGCTC 1 cut(s) 2896
BsrFI RCCGGY 1 cut(s) 622
BsrGI TGTACA 1 cut(s) 1843
BssAI RCCGGY 1 cut(s) 622
BssNI GRCGYC 1 cut(s) 3147
BssT1I CCWWGG 2 cut(s) 32, 3340
Bst4CI ACNGT 8 cut(s) 47, 408, 867, 1588, 2474, 2566, 2785, 3092
Bst6I CTCTTC 2 cut(s) 976, 2089
BstACI GRCGYC 1 cut(s) 3147
BstAPI GCANNNNNTGC 4 cut(s) 278, 748, 770, 1694
BstAUI TGTACA 1 cut(s) 1843
BstBAI YACGTR 1 cut(s) 728
BstC8I GCNNGC 9 cut(s) 152, 568, 830, 1206, 1466, 1635, 1702, 2682, 2718
BstDSI CCRYGG 2 cut(s) 2145, 3340
BstEII GGTNACC 1 cut(s) 2590
BstENI CCTNNNNNAGG 2 cut(s) 21, 534
BstF5I GGATG 6 cut(s) 218, 1320, 1354, 2253, 2527, 2970
BstFNI CGCG 2 cut(s) 1512, 2640
BstH2I RGCGCY 1 cut(s) 2072
BstHHI GCGC 5 cut(s) 289, 596, 1709, 2071, 2642
BstMAI GTCTC 5 cut(s) 162, 1224, 1496, 1862, 2607
BstNSI RCATGY 3 cut(s) 130, 832, 3374
BstPAI GACNNNNGTC 1 cut(s) 3333
BstPI GGTNACC 1 cut(s) 2590
BstSFI CTRYAG 4 cut(s) 324, 1695, 1811, 3185
BstSLI GKGCMC 7 cut(s) 31, 67, 274, 585, 1268, 1810, 2722
BstUI CGCG 2 cut(s) 1512, 2640
BstV2I GAAGAC 2 cut(s) 393, 3333
BstX2I RGATCY 4 cut(s) 698, 782, 3008, 3289
BstXI CCANNNNNNTGG 2 cut(s) 870, 2968
BstYI RGATCY 4 cut(s) 698, 782, 3008, 3289
Bsu15I ATCGAT 1 cut(s) 1656
BsuI GTATCC 2 cut(s) 1672, 2967
BsuTUI ATCGAT 1 cut(s) 1656
BtgI CCRYGG 2 cut(s) 2145, 3340
BtrI CACGTC 1 cut(s) 2700
BtsCI GGATG 6 cut(s) 218, 1320, 1354, 2253, 2527, 2970
BtsI GCAGTG 5 cut(s) 1287, 1519, 1808, 2379, 3153
BveI ACCTGC 3 cut(s) 331, 2266, 3270
Cac8I GCNNGC 9 cut(s) 152, 568, 830, 1206, 1466, 1635, 1702, 2682, 2718
CciI TCATGA 1 cut(s) 1443
CfoI GCGC 5 cut(s) 289, 596, 1709, 2071, 2642
Cfr10I RCCGGY 1 cut(s) 622
Cfr9I CCCGGG 1 cut(s) 265
ClaI ATCGAT 1 cut(s) 1656
CseI GACGC 1 cut(s) 3136
CsiI ACCWGGT 1 cut(s) 274
Csp6I GTAC 8 cut(s) 1157, 1642, 1844, 2013, 2416, 2862, 2933, 3368
CspAI ACCGGT 1 cut(s) 622
CspCI CAANNNNNGTGG 4 cut(s) 696, 731, 1468, 1503
CviQI GTAC 8 cut(s) 1157, 1642, 1844, 2013, 2416, 2862, 2933, 3368
DraI TTTAAA 1 cut(s) 2160
DraIII CACNNNGTG 1 cut(s) 278
EaeI YGGCCR 5 cut(s) 229, 315, 394, 872, 2572
Eam1104I CTCTTC 2 cut(s) 976, 2089
EarI CTCTTC 2 cut(s) 976, 2089
EciI GGCGGA 1 cut(s) 1191
Eco130I CCWWGG 2 cut(s) 32, 3340
Eco24I GRGCYC 1 cut(s) 2127
Eco47I GGWCC 9 cut(s) 506, 539, 551, 653, 758, 818, 1142, 1532, 1742
Eco47III AGCGCT 1 cut(s) 2070
Eco57I CTGAAG 7 cut(s) 696, 1137, 1758, 2094, 2109, 2843, 3279
Eco72I CACGTG 1 cut(s) 728
Eco88I CYCGRG 1 cut(s) 265
Eco91I GGTNACC 1 cut(s) 2590
EcoNI CCTNNNNNAGG 2 cut(s) 21, 534
EcoO109I RGGNCCY 3 cut(s) 539, 818, 1742
EcoO65I GGTNACC 1 cut(s) 2590
EcoRI GAATTC 1 cut(s) 2583
EcoT14I CCWWGG 2 cut(s) 32, 3340
EcoT22I ATGCAT 1 cut(s) 744
EcoT38I GRGCYC 1 cut(s) 2127
ErhI CCWWGG 2 cut(s) 32, 3340
FaqI GGGAC 3 cut(s) 2691, 2811, 3321
FauI CCCGC 1 cut(s) 1517
FblI GTMKAC 1 cut(s) 2454
FokI GGATG 6 cut(s) 205, 1327, 1341, 2240, 2534, 2977
FriOI GRGCYC 1 cut(s) 2127
GlaI GCGC 5 cut(s) 288, 595, 1708, 2070, 2641
GsaI CCCAGC 2 cut(s) 470, 1104
GsuI CTGGAG 3 cut(s) 6, 2037, 2123
HaeII RGCGCY 1 cut(s) 2072
HapII CCGG 8 cut(s) 120, 266, 314, 599, 623, 2268, 2865, 3023
HgaI GACGC 1 cut(s) 3136
HhaI GCGC 5 cut(s) 289, 596, 1709, 2071, 2642
Hin1I GRCGYC 1 cut(s) 3147
Hin6I GCGC 5 cut(s) 287, 594, 1707, 2069, 2640
HinP1I GCGC 5 cut(s) 287, 594, 1707, 2069, 2640
HincII GTYRAC 2 cut(s) 1904, 2455
HindII GTYRAC 2 cut(s) 1904, 2455
HinfI GANTC 8 cut(s) 110, 139, 1504, 1775, 1964, 3028, 3119, 3334
HpaII CCGG 8 cut(s) 120, 266, 314, 599, 623, 2268, 2865, 3023
HphI GGTGA 8 cut(s) 246, 396, 696, 1403, 3014, 3037, 3095, 3142
Hpy188I TCNGA 9 cut(s) 99, 1229, 1278, 1363, 1749, 1894, 1947, 2311, 2530
HpyAV CCTTC 8 cut(s) 253, 540, 1358, 1733, 2084, 2113, 2305, 3303
HpyCH4III ACNGT 8 cut(s) 47, 408, 867, 1588, 2474, 2566, 2785, 3092
HpyCH4IV ACGT 7 cut(s) 727, 1252, 1546, 1804, 2699, 3048, 3102
HpySE526I ACGT 7 cut(s) 727, 1252, 1546, 1804, 2699, 3048, 3102
Hsp92I GRCGYC 1 cut(s) 3147
HspAI GCGC 5 cut(s) 287, 594, 1707, 2069, 2640
LguI GCTCTTC 2 cut(s) 976, 2089
LweI GCATC 7 cut(s) 1305, 1543, 1815, 2367, 2378, 2754, 2977
MabI ACCWGGT 1 cut(s) 274
MaeII ACGT 7 cut(s) 727, 1252, 1546, 1804, 2699, 3048, 3102
MaeIII GTNAC 8 cut(s) 47, 122, 1253, 2347, 2474, 2590, 2669, 3148
MbiI CCGCTC 1 cut(s) 2896
MfeI CAATTG 2 cut(s) 1916, 2172
MflI RGATCY 4 cut(s) 698, 782, 3008, 3289
MlsI TGGCCA 4 cut(s) 231, 396, 874, 2574
MluNI TGGCCA 4 cut(s) 231, 396, 874, 2574
MlyI GAGTC 3 cut(s) 1498, 1769, 3343
MmeI TCCRAC 5 cut(s) 676, 692, 758, 1958, 2468
Mox20I TGGCCA 4 cut(s) 231, 396, 874, 2574
Mph1103I ATGCAT 1 cut(s) 744
MroXI GAANNNNTTC 1 cut(s) 1929
MscI TGGCCA 4 cut(s) 231, 396, 874, 2574
MseI TTAA 5 cut(s) 144, 2159, 2498, 2852, 3095
MslI CAYNNNNRTG 4 cut(s) 868, 2564, 2618, 3051
Msp20I TGGCCA 4 cut(s) 231, 396, 874, 2574
MspA1I CMGCKG 4 cut(s) 1039, 1637, 2114, 3139
MspI CCGG 8 cut(s) 120, 266, 314, 599, 623, 2268, 2865, 3023
MunI CAATTG 2 cut(s) 1916, 2172
Mva1269I GAATGC 5 cut(s) 742, 1029, 1675, 2439, 3302
MvnI CGCG 2 cut(s) 1512, 2640
NciI CCSGG 4 cut(s) 266, 267, 314, 600
NcoI CCATGG 1 cut(s) 3340
NheI GCTAGC 1 cut(s) 566
NlaIV GGNNCC 8 cut(s) 30, 66, 273, 361, 720, 1368, 2124, 3346
NmeAIII GCCGAG 2 cut(s) 277, 1114
NmuCI GTSAC 3 cut(s) 2347, 2474, 3148
NsiI ATGCAT 1 cut(s) 744
NspI RCATGY 3 cut(s) 130, 832, 3374
OliI CACNNNNGTG 2 cut(s) 2564, 3051
PaeI GCATGC 1 cut(s) 832
PagI TCATGA 1 cut(s) 1443
PasI CCCWGGG 1 cut(s) 428
PciI ACATGT 1 cut(s) 3370
PciSI GCTCTTC 2 cut(s) 976, 2089
PcsI WCGNNNNNNNCGW 1 cut(s) 1414
PctI GAATGC 5 cut(s) 742, 1029, 1675, 2439, 3302
PdmI GAANNNNTTC 1 cut(s) 1929
PfeI GAWTC 5 cut(s) 110, 139, 1964, 3028, 3119
PflMI CCANNNNNTGG 4 cut(s) 392, 695, 2057, 2146
PinAI ACCGGT 1 cut(s) 622
PleI GAGTC 3 cut(s) 1498, 1769, 3342
PmaCI CACGTG 1 cut(s) 728
PmlI CACGTG 1 cut(s) 728
PpsI GAGTC 3 cut(s) 1498, 1769, 3342
Ppu21I YACGTR 1 cut(s) 728
PpuMI RGGWCCY 3 cut(s) 539, 818, 1742
PscI ACATGT 1 cut(s) 3370
PshAI GACNNNNGTC 1 cut(s) 3333
PshBI ATTAAT 1 cut(s) 2852
Psp5II RGGWCCY 3 cut(s) 539, 818, 1742
PspCI CACGTG 1 cut(s) 728
PspEI GGTNACC 1 cut(s) 2590
PspFI CCCAGC 2 cut(s) 466, 1100
PspN4I GGNNCC 8 cut(s) 30, 66, 273, 361, 720, 1368, 2124, 3346
PspPPI RGGWCCY 3 cut(s) 539, 818, 1742
PstI CTGCAG 4 cut(s) 328, 1699, 1815, 3189
PsuI RGATCY 4 cut(s) 698, 782, 3008, 3289
PvuII CAGCTG 3 cut(s) 1637, 2114, 3139
RsaI GTAC 8 cut(s) 1158, 1643, 1845, 2014, 2417, 2863, 2934, 3369
RsaNI GTAC 8 cut(s) 1157, 1642, 1844, 2013, 2416, 2862, 2933, 3368
RseI CAYNNNNRTG 4 cut(s) 868, 2564, 2618, 3051
SalI GTCGAC 1 cut(s) 2453
SapI GCTCTTC 2 cut(s) 976, 2089
SaqAI TTAA 5 cut(s) 144, 2159, 2498, 2852, 3095
ScaI AGTACT 3 cut(s) 1158, 2014, 2417
SchI GAGTC 3 cut(s) 1498, 1769, 3343
SexAI ACCWGGT 1 cut(s) 274
SfaNI GCATC 7 cut(s) 1305, 1543, 1815, 2367, 2378, 2754, 2977
SfcI CTRYAG 4 cut(s) 324, 1695, 1811, 3185
SinI GGWCC 9 cut(s) 506, 539, 551, 653, 758, 818, 1142, 1532, 1742
SmaI CCCGGG 1 cut(s) 267
SmiMI CAYNNNNRTG 4 cut(s) 868, 2564, 2618, 3051
SmlI CTYRAG 2 cut(s) 822, 3074
SmoI CTYRAG 2 cut(s) 822, 3074
SpeI ACTAGT 1 cut(s) 2615
SphI GCATGC 1 cut(s) 832
StyI CCWWGG 2 cut(s) 32, 3340
TaaI ACNGT 8 cut(s) 47, 408, 867, 1588, 2474, 2566, 2785, 3092
TaiI ACGT 7 cut(s) 730, 1255, 1549, 1807, 2702, 3051, 3105
TaqI TCGA 4 cut(s) 1408, 1656, 1778, 2454
TatI WGTACW 5 cut(s) 1156, 1641, 1843, 2012, 2415
TauI GCSGC 6 cut(s) 241, 368, 835, 2359, 2687, 3246
TfiI GAWTC 5 cut(s) 110, 139, 1964, 3028, 3119
Tru1I TTAA 5 cut(s) 144, 2159, 2498, 2852, 3095
Tru9I TTAA 5 cut(s) 144, 2159, 2498, 2852, 3095
TseFI GTSAC 3 cut(s) 2347, 2474, 3148
Tsp45I GTSAC 3 cut(s) 2347, 2474, 3148
TspGWI ACGGA 1 cut(s) 2593
TspMI CCCGGG 1 cut(s) 265
Van91I CCANNNNNTGG 4 cut(s) 392, 695, 2057, 2146
VneI GTGCAC 3 cut(s) 1264, 1806, 2718
VpaK11BI GGWCC 9 cut(s) 506, 539, 551, 653, 758, 818, 1142, 1532, 1742
VspI ATTAAT 1 cut(s) 2852
XagI CCTNNNNNAGG 2 cut(s) 21, 534
XapI RAATTY 8 cut(s) 133, 676, 1128, 1592, 2177, 2583, 2771, 3126
XbaI TCTAGA 2 cut(s) 416, 1864
XceI RCATGY 3 cut(s) 130, 832, 3374
XcmI CCANNNNNNNNNTGG 1 cut(s) 3338
XmaI CCCGGG 1 cut(s) 265
XmaJI CCTAGG 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 2454
XmnI GAANNNNTTC 1 cut(s) 1929
ZrmI AGTACT 3 cut(s) 1158, 2014, 2417
Zsp2I ATGCAT 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.