Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008436

Physical Location & Seq

Genomic Coordinates Forward (+)

2477946 .. 2482130

Gene Structure 4185 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1034700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCGCGGGAAAATCCGACGGAGAGTGCCCTATGTCTGCTCCGGACCCGGCTCTGCGACCCAAATTTCGCCTTCAAAGCTCTCTCTCACTCCGACGACTCAAACTACAGCAAGTTGAAATTTTTAGTTTCGAGTTCGATCACTGAGGCCTGCAACAACTCCATTTTGCTTCTGGGTCCCCGTGGTTCCGGAAAAATTGCTGTTTGCCCCCCCAAATTTTCCATTGTAGTTCCTCTTATAATTGTATTGGAGCTTGTGCTCAGCGATTTATTACAAGAATTTCCTGATATGATTTCAGTGATTAGGTTGAGTGGCCTTTTACACAGCAGTGACAACTGCGCATTCAAGGAAATCGCTAGGCAGCTATGCGTGGAGCATCAGTTGGTGTTCTCGAGAATGGCATCGTTTGATGATAACTCTCAGTTTATGATAGCCATGTTAAGGGAGTGTGGATTAGCGCATAAAACAATTATTTTTGTGTTGGATGAGTTTGACTTGTTTGCTCTGGGAAAACAGAGATTGCTTTATAGTTTACTTGATGCAATGCAGTCAGTTACATCTCAGGCTGTTGTCATTGGTGTGAGTTCGCGACTGGATGCTGATATGCTTTTGGACAAAAGAGTAAGATCTCGTTTTTCACATAGAAAGCTTTTGTTTCTTCCTCCTGCTTATGAAGACATACAACGATTGTTGGAGCACATTTTACTTTTGCCAACAGATTCAAGCTTTCCACCAGACTATGTTATTGCGTTCAATGCAAAACTTCAAACTATATTGGCCGATGAGAGATTCAAAGAGATTATTAATACATATCTGAATCTTGATTCAACCGTCAAACATTTGTTAAGATATCTATTCCGCGCTGTTTCTGATATGGATTTAGAATCTGGAATGCTGTCGCTAGAAAACTTCAAAACTGCACTTTCAAATATCCAAAGGCATCCAAAGTTGGAATGCATAAAAGATTGCTCTGTATTGGAACTTTACATTCTGGTATGCATGAAGAGGTTGGAAGTTAAGGAGCAGAACTCATACAATTTCAACGCTGTAATGAAAGAGTACAAAAGCATACACGATTCGGTCAAGACAGCTGACTATTTTGCAAGAAATGTTTGCTTACGGGCATTCGAACACCTTCTACAACGTGAACTAATTGGGTTTGCAGACAACAGAGGACAGAATCAATCCATTGAATTCAGTCCTGTAAAACTTTTAATCTCATCCCATGAACTACATCAGGGGATGAAATCATACGGTTCCTGTCGCAATTCTTCAACAATTAATGGATCGTCAAAAGTAGAGTTGCTGAAGGGAGCTGCTTCATGTGTATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000228 GO:0000808 GO:0003674 GO:0003676 GO:0003677 GO:0003688 GO:0003690 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005664 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009743 GO:0009744 GO:0009987 GO:0010033 GO:0031974 GO:0031981 GO:0032991 GO:0034285 GO:0034641 GO:0034645 GO:0036094 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:1901265 GO:1901360 GO:1901363 GO:1901576 GO:1901700 GO:1990837

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 map04110 map04111 map04113

Pfam Domains

Protein Families

AAA AAA_16 ORC4_C

Protein Analysis

445

Amino Acids

50.15

Weight (kDa)

7.15

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ORC4_C	PF14629	230 - 414	1.9e-41	Origin recognition complex (ORC) subunit 4 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008436)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01120 AT2G01120
fragaria_vesca	FvH4_4g26400 FvH4_4g26400 FvH4_4g26400
malus_domestica	MD13G1032700.v1.1 MD16G1034700.v1.1
prunus_persica	Prupe.1G319900_v2.0.a1 Prupe.1G319900_v2.0.a1 Prupe.1G319900_v2.0.a1 Prupe.1G319900_v2.0.a1
pyrus_communis	pycom13g02820 pycom16g02950
rosa_chinensis	RchiOBHm_Chr4g0434031
rosa_laevigata	RLG00000006707
rosa_multiflora	Rmu_sc0000352.1_g000025
rosa_roxburghii	Rroxscaffold_5G00375440
rosa_rugosa	Rorug04G0272800
rosa_samantha	Rh4AG328500 Rh4BG335800 Rh4CG351300 Rh4DG332200
rosa_wichuraiana	Rw4G028480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	239
Acc16I	TGCGCA	1 cut(s)	340
AccII	CGCG	3 cut(s)	7, 589, 861
AccIII	TCCGGA	2 cut(s)	42, 188
AciI	CCGC	2 cut(s)	7, 859
AclWI	GGATC	1 cut(s)	1294
AcoI	YGGCCR	1 cut(s)	777
AcsI	RAATTY	5 cut(s)	64, 119, 215, 278, 1193
AcuI	CTGAAG	1 cut(s)	1328
AfaI	GTAC	1 cut(s)	1061
AfiI	CCNNNNNNNGG	1 cut(s)	441
AleI	CACNNNNGTG	1 cut(s)	327
AluBI	AGCT	7 cut(s)	80, 253, 364, 649, 726, 1091, 1316
AluI	AGCT	7 cut(s)	80, 253, 364, 649, 726, 1091, 1316
Alw21I	GWGCWC	2 cut(s)	261, 699
AlwI	GGATC	1 cut(s)	1294
Ama87I	CYCGRG	1 cut(s)	391
Aor13HI	TCCGGA	2 cut(s)	42, 188
AoxI	GGCC	3 cut(s)	147, 313, 777
ApeKI	GCWGC	2 cut(s)	361, 1316
ApoI	RAATTY	5 cut(s)	64, 119, 215, 278, 1193
ArsI	GACNNNNNNTTYG	2 cut(s)	50, 82
AseI	ATTAAT	2 cut(s)	804, 1281
Asp700I	GAANNNNTTC	1 cut(s)	1134
AspLEI	GCGC	4 cut(s)	7, 341, 460, 863
AspS9I	GGNCC	2 cut(s)	45, 176
AsuC2I	CCSGG	1 cut(s)	49
AsuII	TTCGAA	1 cut(s)	1128
AvaI	CYCGRG	1 cut(s)	391
AvaII	GGWCC	2 cut(s)	45, 176
BaeGI	GKGCMC	1 cut(s)	31
BbsI	GAAGAC	1 cut(s)	681
Bbv12I	GWGCWC	2 cut(s)	261, 699
BbvI	GCAGC	2 cut(s)	373, 1303
BcnI	CCSGG	1 cut(s)	49
BfaI	CTAG	2 cut(s)	357, 902
BfmI	CTRYAG	1 cut(s)	106
BglII	AGATCT	1 cut(s)	626
BisI	GCNGC	2 cut(s)	362, 1317
BlpI	GCTNAGC	1 cut(s)	260
BlsI	GCNGC	2 cut(s)	363, 1318
Bme1390I	CCNGG	1 cut(s)	49
Bme18I	GGWCC	2 cut(s)	45, 176
BmeT110I	CYCGRG	1 cut(s)	391
BmgT120I	GGNCC	2 cut(s)	45, 176
BmiI	GGNNCC	5 cut(s)	47, 177, 178, 187, 1258
BmrFI	CCNGG	1 cut(s)	49
BmsI	GCATC	5 cut(s)	385, 410, 529, 586, 949
BpiI	GAAGAC	1 cut(s)	681
BplI	GAGNNNNNCTC	2 cut(s)	1013, 1045
Bpu1102I	GCTNAGC	1 cut(s)	260
Bpu14I	TTCGAA	1 cut(s)	1128
BpuMI	CCSGG	1 cut(s)	49
BsaJI	CCNNGG	1 cut(s)	181
BsaWI	WCCGGW	2 cut(s)	42, 188
BsaXI	ACNNNNNCTCC	2 cut(s)	365, 395
Bsc4I	CCNNNNNNNGG	1 cut(s)	441
Bse1I	ACTGG	1 cut(s)	597
Bse3DI	GCAATG	1 cut(s)	549
BseAI	TCCGGA	2 cut(s)	42, 188
BseDI	CCNNGG	1 cut(s)	181
BseGI	GGATG	5 cut(s)	490, 601, 940, 1220, 1248
BseLI	CCNNNNNNNGG	1 cut(s)	441
BseMI	GCAATG	1 cut(s)	549
BseMII	CTCAG	4 cut(s)	135, 274, 434, 575
BseNI	ACTGG	1 cut(s)	597
BseSI	GKGCMC	1 cut(s)	31
BseXI	GCAGC	2 cut(s)	373, 1303
BsgI	GTGCAG	1 cut(s)	903
Bsh1236I	CGCG	3 cut(s)	7, 589, 861
BshFI	GGCC	3 cut(s)	149, 315, 779
BsiHKAI	GWGCWC	2 cut(s)	261, 699
BsiHKCI	CYCGRG	1 cut(s)	391
BsiSI	CCGG	3 cut(s)	43, 49, 189
BslFI	GGGAC	1 cut(s)	162
BslI	CCNNNNNNNGG	1 cut(s)	441
BsmFI	GGGAC	1 cut(s)	162
BsmI	GAATGC	4 cut(s)	341, 897, 959, 1124
BsnI	GGCC	3 cut(s)	149, 315, 779
BsoBI	CYCGRG	1 cut(s)	391
Bsp119I	TTCGAA	1 cut(s)	1128
Bsp1286I	GDGCHC	3 cut(s)	31, 261, 699
Bsp13I	TCCGGA	2 cut(s)	42, 188
Bsp143I	GATC	3 cut(s)	138, 626, 1286
Bsp1720I	GCTNAGC	1 cut(s)	260
Bsp68I	TCGCGA	1 cut(s)	589
BspACI	CCGC	2 cut(s)	7, 859
BspANI	GGCC	3 cut(s)	149, 315, 779
BspCNI	CTCAG	4 cut(s)	136, 273, 433, 574
BspEI	TCCGGA	2 cut(s)	42, 188
BspFNI	CGCG	3 cut(s)	7, 589, 861
BspLI	GGNNCC	5 cut(s)	47, 177, 178, 187, 1258
BspPI	GGATC	1 cut(s)	1294
BspT104I	TTCGAA	1 cut(s)	1128
BsrDI	GCAATG	1 cut(s)	549
BsrI	ACTGG	1 cut(s)	597
BssECI	CCNNGG	1 cut(s)	181
BssMI	GATC	3 cut(s)	138, 626, 1286
Bst4CI	ACNGT	2 cut(s)	832, 1256
Bst6I	CTCTTC	1 cut(s)	998
BstBI	TTCGAA	1 cut(s)	1128
BstC8I	GCNNGC	1 cut(s)	151
BstDEI	CTNAG	4 cut(s)	144, 260, 420, 561
BstDSI	CCRYGG	1 cut(s)	181
BstF5I	GGATG	5 cut(s)	490, 601, 940, 1220, 1248
BstFNI	CGCG	3 cut(s)	7, 589, 861
BstHHI	GCGC	4 cut(s)	7, 341, 460, 863
BstKTI	GATC	3 cut(s)	141, 629, 1289
BstMBI	GATC	3 cut(s)	138, 626, 1286
BstMWI	GCNNNNNNNGC	2 cut(s)	77, 755
BstSCI	CCNGG	1 cut(s)	47
BstSFI	CTRYAG	1 cut(s)	106
BstSLI	GKGCMC	1 cut(s)	31
BstUI	CGCG	3 cut(s)	7, 589, 861
BstV1I	GCAGC	2 cut(s)	373, 1303
BstV2I	GAAGAC	1 cut(s)	681
BstX2I	RGATCY	1 cut(s)	626
BstYI	RGATCY	1 cut(s)	626
BsuRI	GGCC	3 cut(s)	149, 315, 779
BtgI	CCRYGG	1 cut(s)	181
BtsCI	GGATG	5 cut(s)	490, 601, 940, 1220, 1248
BtsI	GCAGTG	1 cut(s)	334
BtsIMutI	CAGTG	3 cut(s)	141, 303, 334
BtuMI	TCGCGA	1 cut(s)	589
Cac8I	GCNNGC	1 cut(s)	151
CfoI	GCGC	4 cut(s)	7, 341, 460, 863
Cfr13I	GGNCC	2 cut(s)	45, 176
Csp6I	GTAC	1 cut(s)	1060
CviAII	CATG	4 cut(s)	436, 1000, 1226, 1323
CviQI	GTAC	1 cut(s)	1060
DdeI	CTNAG	4 cut(s)	144, 260, 420, 561
DpnI	GATC	3 cut(s)	140, 628, 1288
DpnII	GATC	3 cut(s)	138, 626, 1286
EaeI	YGGCCR	1 cut(s)	777
Eam1104I	CTCTTC	1 cut(s)	998
EarI	CTCTTC	1 cut(s)	998
Eco147I	AGGCCT	1 cut(s)	149
Eco32I	GATATC	1 cut(s)	851
Eco47I	GGWCC	2 cut(s)	45, 176
Eco57I	CTGAAG	1 cut(s)	1328
Eco88I	CYCGRG	1 cut(s)	391
EcoO109I	RGGNCCY	1 cut(s)	176
EcoRI	GAATTC	1 cut(s)	1193
EcoRV	GATATC	1 cut(s)	851
EcoT22I	ATGCAT	2 cut(s)	959, 1001
FaeI	CATG	4 cut(s)	439, 1003, 1229, 1326
FaqI	GGGAC	1 cut(s)	162
FatI	CATG	4 cut(s)	435, 999, 1225, 1322
Fnu4HI	GCNGC	2 cut(s)	362, 1317
FokI	GGATG	5 cut(s)	497, 608, 927, 1207, 1255
Fsp4HI	GCNGC	2 cut(s)	362, 1317
FspBI	CTAG	2 cut(s)	357, 902
FspI	TGCGCA	1 cut(s)	340
GlaI	GCGC	4 cut(s)	6, 340, 459, 862
GluI	GCNGC	2 cut(s)	362, 1317
HaeIII	GGCC	3 cut(s)	149, 315, 779
HapII	CCGG	3 cut(s)	43, 49, 189
HhaI	GCGC	4 cut(s)	7, 341, 460, 863
Hin1II	CATG	4 cut(s)	439, 1003, 1229, 1326
Hin6I	GCGC	4 cut(s)	5, 339, 458, 861
HinP1I	GCGC	4 cut(s)	5, 339, 458, 861
HindIII	AAGCTT	2 cut(s)	647, 724
HinfI	GANTC	8 cut(s)	98, 719, 789, 817, 824, 884, 1076, 1180
HpaII	CCGG	3 cut(s)	43, 49, 189
Hpy166II	GTNNAC	2 cut(s)	533, 1148
Hpy188I	TCNGA	4 cut(s)	18, 94, 816, 871
Hpy188III	TCNNGA	9 cut(s)	43, 189, 284, 391, 393, 588, 821, 888, 1084
Hpy8I	GTNNAC	2 cut(s)	533, 1148
Hpy99I	CGWCG	2 cut(s)	22, 98
HpyAV	CCTTC	3 cut(s)	82, 1145, 1303
HpyCH4III	ACNGT	2 cut(s)	832, 1256
HpyCH4IV	ACGT	1 cut(s)	1144
HpyCH4V	TGCA	9 cut(s)	153, 542, 547, 758, 920, 957, 999, 1103, 1163
HpyF10VI	GCNNNNNNNGC	2 cut(s)	77, 755
HpyF3I	CTNAG	4 cut(s)	144, 260, 420, 561
HpySE526I	ACGT	1 cut(s)	1144
Hsp92II	CATG	4 cut(s)	439, 1003, 1229, 1326
HspAI	GCGC	4 cut(s)	5, 339, 458, 861
KflI	GGGWCCC	1 cut(s)	176
Kpn2I	TCCGGA	2 cut(s)	42, 188
Kzo9I	GATC	3 cut(s)	138, 626, 1286
LmnI	GCTCC	6 cut(s)	45, 250, 373, 694, 1021, 1313
Lsp1109I	GCAGC	2 cut(s)	373, 1303
LweI	GCATC	5 cut(s)	385, 410, 529, 586, 949
MaeI	CTAG	2 cut(s)	357, 902
MaeII	ACGT	1 cut(s)	1144
MaeIII	GTNAC	2 cut(s)	329, 553
MalI	GATC	3 cut(s)	140, 628, 1288
MboI	GATC	3 cut(s)	138, 626, 1286
MboII	GAAGA	4 cut(s)	650, 686, 1015, 1263
MflI	RGATCY	1 cut(s)	626
MhlI	GDGCHC	3 cut(s)	31, 261, 699
MlyI	GAGTC	1 cut(s)	92
MmeI	TCCRAC	6 cut(s)	41, 117, 462, 672, 930, 990
MnlI	CCTC	5 cut(s)	139, 243, 672, 999, 1166
Mph1103I	ATGCAT	2 cut(s)	959, 1001
MroI	TCCGGA	2 cut(s)	42, 188
MroXI	GAANNNNTTC	1 cut(s)	1134
MseI	TTAA	6 cut(s)	440, 804, 845, 1017, 1214, 1281
MslI	CAYNNNNRTG	2 cut(s)	327, 578
MspA1I	CMGCKG	1 cut(s)	1091
MspI	CCGG	3 cut(s)	43, 49, 189
MspR9I	CCNGG	1 cut(s)	49
Mva1269I	GAATGC	4 cut(s)	341, 897, 959, 1124
MvnI	CGCG	3 cut(s)	7, 589, 861
MwoI	GCNNNNNNNGC	2 cut(s)	77, 755
NciI	CCSGG	1 cut(s)	49
NdeII	GATC	3 cut(s)	138, 626, 1286
NlaIII	CATG	4 cut(s)	439, 1003, 1229, 1326
NlaIV	GGNNCC	5 cut(s)	47, 177, 178, 187, 1258
NmuCI	GTSAC	1 cut(s)	329
NruI	TCGCGA	1 cut(s)	589
NsbI	TGCGCA	1 cut(s)	340
NsiI	ATGCAT	2 cut(s)	959, 1001
NspV	TTCGAA	1 cut(s)	1128
OliI	CACNNNNGTG	1 cut(s)	327
PaeR7I	CTCGAG	1 cut(s)	391
PceI	AGGCCT	1 cut(s)	149
PctI	GAATGC	4 cut(s)	341, 897, 959, 1124
PdmI	GAANNNNTTC	1 cut(s)	1134
PfeI	GAWTC	7 cut(s)	719, 789, 817, 824, 884, 1076, 1180
PkrI	GCNGC	2 cut(s)	363, 1318
PleI	GAGTC	1 cut(s)	92
PpsI	GAGTC	1 cut(s)	92
PpuMI	RGGWCCY	1 cut(s)	176
PshBI	ATTAAT	2 cut(s)	804, 1281
PsiI	TTATAA	1 cut(s)	239
Psp5II	RGGWCCY	1 cut(s)	176
PspN4I	GGNNCC	5 cut(s)	47, 177, 178, 187, 1258
PspPI	GGNCC	2 cut(s)	45, 176
PspPPI	RGGWCCY	1 cut(s)	176
PsrI	GAACNNNNNNTAC	2 cut(s)	1122, 1154
PsuI	RGATCY	1 cut(s)	626
PvuII	CAGCTG	1 cut(s)	1091
RruI	TCGCGA	1 cut(s)	589
RsaI	GTAC	1 cut(s)	1061
RsaNI	GTAC	1 cut(s)	1060
RseI	CAYNNNNRTG	2 cut(s)	327, 578
SaqAI	TTAA	6 cut(s)	440, 804, 845, 1017, 1214, 1281
SatI	GCNGC	2 cut(s)	362, 1317
Sau3AI	GATC	3 cut(s)	138, 626, 1286
Sau96I	GGNCC	2 cut(s)	45, 176
SchI	GAGTC	1 cut(s)	92
ScrFI	CCNGG	1 cut(s)	49
SduI	GDGCHC	3 cut(s)	31, 261, 699
SfaNI	GCATC	5 cut(s)	385, 410, 529, 586, 949
SfcI	CTRYAG	1 cut(s)	106
Sfr274I	CTCGAG	1 cut(s)	391
SfuI	TTCGAA	1 cut(s)	1128
SinI	GGWCC	2 cut(s)	45, 176
SlaI	CTCGAG	1 cut(s)	391
SmiMI	CAYNNNNRTG	2 cut(s)	327, 578
SmlI	CTYRAG	1 cut(s)	391
SmoI	CTYRAG	1 cut(s)	391
SseBI	AGGCCT	1 cut(s)	149
SsiI	CCGC	2 cut(s)	7, 859
SspMI	CTAG	2 cut(s)	357, 902
StuI	AGGCCT	1 cut(s)	149
StyD4I	CCNGG	1 cut(s)	47
TaaI	ACNGT	2 cut(s)	832, 1256
TaiI	ACGT	1 cut(s)	1147
TaqI	TCGA	4 cut(s)	131, 137, 392, 1128
TaqII	GACCGA	1 cut(s)	1069
TatI	WGTACW	1 cut(s)	1059
TfiI	GAWTC	7 cut(s)	719, 789, 817, 824, 884, 1076, 1180
Tru1I	TTAA	6 cut(s)	440, 804, 845, 1017, 1214, 1281
Tru9I	TTAA	6 cut(s)	440, 804, 845, 1017, 1214, 1281
TscAI	CASTG	3 cut(s)	148, 303, 334
TseFI	GTSAC	1 cut(s)	329
TseI	GCWGC	2 cut(s)	361, 1316
Tsp45I	GTSAC	1 cut(s)	329
TspDTI	ATGAA	6 cut(s)	687, 1016, 1067, 1242, 1259, 1311
TspGWI	ACGGA	1 cut(s)	35
TspRI	CASTG	3 cut(s)	148, 303, 334
VpaK11BI	GGWCC	2 cut(s)	45, 176
VspI	ATTAAT	2 cut(s)	804, 1281
XapI	RAATTY	5 cut(s)	64, 119, 215, 278, 1193
XcmI	CCANNNNNNNNNTGG	1 cut(s)	169
XhoI	CTCGAG	1 cut(s)	391
XmnI	GAANNNNTTC	1 cut(s)	1134
XspI	CTAG	2 cut(s)	357, 902
Zsp2I	ATGCAT	2 cut(s)	959, 1001

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.