RchiOBHm_Chr4g0434031

Component of the origin recognition complex (ORC) that binds origins of replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008436
Physical Location & Seq
Forward (+)
57936208 .. 57940749
4542 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40250

Sequence Viewer

Length: 1248 bp
ATGGGGCGAGAAAGTCCGGCGGAAAAAGCCCTAATTCTTCTGAGGACCAGACTCTGTGACCCGAATTTCATTTTCCGGCCGCTCTGTGAATCTTCCGACTCAAATTACAGCAAGCTGAAGTTCTTAGTGTCGAGTTCGATTACCGAGGCGTGCAATAACTCTATCCTGCTTCTGGGTCCTCGTGGTTCTGGAAAAATTGCCGTATTGGATCTTGTGATCAGTGATTTATTACAAGAGTATCCTGATACCATTACAGTGATTAGGTTGAGTGGCCTTTTGCATAGCGATGAGAATTGTGCGTTAAAGGAAATTTCTAGACAGCTATGTACGGAGCATCAGTTGTTGTTTTCAAAAATGGCATCATTTGATGAGAACTCCCAGTTTCTGATAGCCATGTTACGGGAATGCGGGTTGGCACATAAAACAATCATTTTTGTGCTGGATGAGTTTGACTTATTTGCTCTGGGAAAACAGCGATTACTTTATAATTTGCTTGATGCACTGCAGTCAGTGACATCTCAAGCTGTTGTTATTGGTGTCAGATGCGGATCAGCTTTTGGAAAAAGAGTAATATCTCGATTTTCAAATAGAAAGCTTTTGTTTCTTCCTCCCTCTAAGGAAGACATACAGCGATTGTTGGAGCACATTTTATTATTGCCAACAGACTCGGGCCTTCCTACAGACTATGTTGTTGAGTTCAACACGAAACTTCAAAACATATTGGCTGATGAGAGGTTCGAAGAGATTATAAACACATATCTAAATATTGATTCCACCATCAAAGATTTCTTGAGATATCTATTCCGTTCTGTTTCTAATATGGACTTGCAATCTGGAATGCTGTTGCTTGAAAACTTCAAAACTGCACTTTCCAATATCCAAAGGCATCCAAAGATGGAATGCATAAAAGATTGTTCAATATTGGAACTTTACATTTTGGTATGCATGAAGAGGTTGGAAGTTAAAGAGCACAACTCATACAATTTCACAACTGTAATGAAAGAGTACAAAAGCGTACATGATTCGTTCCAGACATCTGACTATTATGCGAAGAATGTGTGCTTACGGGCATTTGAACACCTTCTGCAACGTGGACTGATTGAATTTATAGACAACAGAGGGCAAAACCAGTCTGTTGAATATCGTCCGGTGAAACTTTTAATCTCATTTCATCAACTACATCAGGGCCTGAAATCATATCGTTCGTGTCCTGCCATTCTTCAGAAAATAATGGATCGTGAAAAATAA

Protein Analysis

415

Amino Acids

47.66

Weight (kDa)

7.53

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ORC4_C PF14629 212 - 396 1.6e-40 Origin recognition complex (ORC) subunit 4 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 486, 749
AccBSI CCGCTC 1 cut(s) 82
AciI CCGC 4 cut(s) 20, 80, 408, 546
AclWI GGATC 3 cut(s) 216, 556, 1242
AcoI YGGCCR 1 cut(s) 77
AcsI RAATTY 3 cut(s) 64, 309, 1103
AcuI CTGAAG 2 cut(s) 137, 1205
AfaI GTAC 3 cut(s) 328, 1007, 1017
AfiI CCNNNNNNNGG 2 cut(s) 172, 399
AluBI AGCT 5 cut(s) 115, 322, 524, 554, 595
AluI AGCT 5 cut(s) 115, 322, 524, 554, 595
Alw21I GWGCWC 2 cut(s) 645, 972
AlwI GGATC 3 cut(s) 216, 556, 1242
AlwNI CAGNNNCTG 3 cut(s) 54, 385, 1189
Ama87I CYCGRG 1 cut(s) 667
AoxI GGCC 4 cut(s) 77, 271, 670, 1186
ApoI RAATTY 3 cut(s) 64, 309, 1103
Asp700I GAANNNNTTC 1 cut(s) 1080
AspS9I GGNCC 4 cut(s) 45, 176, 670, 1186
AsuHPI GGTGA 1 cut(s) 1162
AsuII TTCGAA 1 cut(s) 738
AvaI CYCGRG 1 cut(s) 667
AvaII GGWCC 2 cut(s) 45, 176
BaeI ACNNNNGTAYC 2 cut(s) 221, 254
BauI CACGAG 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 2 cut(s) 645, 972
BccI CCATC 2 cut(s) 785, 889
BceAI ACGGC 1 cut(s) 185
BciVI GTATCC 1 cut(s) 249
BclI TGATCA 1 cut(s) 216
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 2 cut(s) 503, 678
BfuI GTATCC 1 cut(s) 249
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
Bme18I GGWCC 2 cut(s) 45, 176
BmeT110I CYCGRG 1 cut(s) 667
BmgT120I GGNCC 4 cut(s) 45, 176, 670, 1186
BmiI GGNNCC 1 cut(s) 177
BmrI ACTGGG 1 cut(s) 373
BmsI GCATC 5 cut(s) 343, 368, 487, 533, 895
BmuI ACTGGG 1 cut(s) 373
BpiI GAAGAC 1 cut(s) 627
BplI GAGNNNNNCTC 2 cut(s) 959, 991
Bpu14I TTCGAA 1 cut(s) 738
BpuEI CTTGAG 2 cut(s) 504, 811
BsaBI GATNNNNATC 1 cut(s) 547
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 1147
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 399
Bse1I ACTGG 2 cut(s) 379, 1129
Bse8I GATNNNNATC 1 cut(s) 547
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 2 cut(s) 448, 886
BseJI GATNNNNATC 1 cut(s) 547
BseLI CCNNNNNNNGG 2 cut(s) 172, 399
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 2 cut(s) 379, 1129
BseX3I CGGCCG 1 cut(s) 77
BsgI GTGCAG 1 cut(s) 849
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 4 cut(s) 79, 273, 672, 1188
BsiEI CGRYCG 1 cut(s) 80
BsiHKAI GWGCWC 2 cut(s) 645, 972
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 3 cut(s) 17, 76, 1148
BslI CCNNNNNNNGG 2 cut(s) 172, 399
BsmI GAATGC 3 cut(s) 410, 843, 905
BsnI GGCC 4 cut(s) 79, 273, 672, 1188
BsoBI CYCGRG 1 cut(s) 667
Bsp119I TTCGAA 1 cut(s) 738
Bsp1286I GDGCHC 2 cut(s) 645, 972
Bsp143I GATC 4 cut(s) 208, 216, 548, 1234
BspACI CCGC 4 cut(s) 20, 80, 408, 546
BspANI GGCC 4 cut(s) 79, 273, 672, 1188
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 177
BspMAI CTGCAG 1 cut(s) 507
BspPI GGATC 3 cut(s) 216, 556, 1242
BspT104I TTCGAA 1 cut(s) 738
BsrBI CCGCTC 1 cut(s) 82
BsrI ACTGG 2 cut(s) 379, 1129
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 4 cut(s) 208, 216, 548, 1234
BssSI CACGAG 1 cut(s) 180
Bst2BI CACGAG 1 cut(s) 180
Bst4CI ACNGT 2 cut(s) 256, 994
Bst6I CTCTTC 2 cut(s) 735, 944
BstBI TTCGAA 1 cut(s) 738
BstC8I GCNNGC 2 cut(s) 113, 151
BstDEI CTNAG 3 cut(s) 41, 124, 615
BstF5I GGATG 2 cut(s) 448, 886
BstKTI GATC 4 cut(s) 211, 219, 551, 1237
BstMBI GATC 4 cut(s) 208, 216, 548, 1234
BstMCI CGRYCG 1 cut(s) 80
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstSFI CTRYAG 2 cut(s) 503, 678
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BstZI CGGCCG 1 cut(s) 77
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 4 cut(s) 79, 273, 672, 1188
BtgZI GCGATG 1 cut(s) 300
BtsCI GGATG 2 cut(s) 448, 886
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 4 cut(s) 226, 261, 500, 516
Cac8I GCNNGC 2 cut(s) 113, 151
CaiI CAGNNNCTG 3 cut(s) 54, 385, 1189
Cfr13I GGNCC 4 cut(s) 45, 176, 670, 1186
Csp6I GTAC 3 cut(s) 327, 1006, 1016
CviAII CATG 3 cut(s) 394, 946, 1019
CviQI GTAC 3 cut(s) 327, 1006, 1016
DdeI CTNAG 3 cut(s) 41, 124, 615
DpnI GATC 4 cut(s) 210, 218, 550, 1236
DpnII GATC 4 cut(s) 208, 216, 548, 1234
EaeI YGGCCR 1 cut(s) 77
EagI CGGCCG 1 cut(s) 77
Eam1104I CTCTTC 2 cut(s) 735, 944
EarI CTCTTC 2 cut(s) 735, 944
EciI GGCGGA 1 cut(s) 35
EclXI CGGCCG 1 cut(s) 77
Eco32I GATATC 1 cut(s) 797
Eco47I GGWCC 2 cut(s) 45, 176
Eco52I CGGCCG 1 cut(s) 77
Eco57I CTGAAG 2 cut(s) 137, 1205
Eco88I CYCGRG 1 cut(s) 667
EcoO109I RGGNCCY 2 cut(s) 176, 1186
EcoRV GATATC 1 cut(s) 797
EcoT22I ATGCAT 2 cut(s) 905, 947
FaeI CATG 3 cut(s) 397, 949, 1022
FatI CATG 3 cut(s) 393, 945, 1018
FauI CCCGC 1 cut(s) 401
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 1 cut(s) 80
FokI GGATG 2 cut(s) 455, 873
Fsp4HI GCNGC 1 cut(s) 80
FspBI CTAG 1 cut(s) 315
GluI GCNGC 1 cut(s) 80
HaeIII GGCC 4 cut(s) 79, 273, 672, 1188
HapII CCGG 3 cut(s) 17, 76, 1148
Hin1II CATG 3 cut(s) 397, 949, 1022
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 6 cut(s) 51, 89, 98, 665, 770, 1022
HpaII CCGG 3 cut(s) 17, 76, 1148
HphI GGTGA 1 cut(s) 1162
Hpy166II GTNNAC 1 cut(s) 1094
Hpy188I TCNGA 6 cut(s) 42, 97, 387, 542, 1039, 1224
Hpy188III TCNNGA 8 cut(s) 189, 242, 315, 576, 790, 834, 1030, 1238
Hpy8I GTNNAC 1 cut(s) 1094
HpyAV CCTTC 2 cut(s) 683, 1091
HpyCH4III ACNGT 2 cut(s) 256, 994
HpyCH4IV ACGT 1 cut(s) 1090
HpyCH4V TGCA 9 cut(s) 153, 280, 500, 505, 829, 866, 903, 945, 1087
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 3 cut(s) 41, 124, 615
HpySE526I ACGT 1 cut(s) 1090
Hsp92II CATG 3 cut(s) 397, 949, 1022
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 4 cut(s) 208, 216, 548, 1234
LmnI GCTCC 2 cut(s) 331, 640
LweI GCATC 5 cut(s) 343, 368, 487, 533, 895
MaeI CTAG 1 cut(s) 315
MaeII ACGT 1 cut(s) 1090
MaeIII GTNAC 3 cut(s) 56, 396, 511
MalI GATC 4 cut(s) 210, 218, 550, 1236
MbiI CCGCTC 1 cut(s) 82
MboI GATC 4 cut(s) 208, 216, 548, 1234
MboII GAAGA 8 cut(s) 29, 84, 596, 632, 752, 961, 1063, 1211
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 2 cut(s) 645, 972
MluCI AATT 9 cut(s) 33, 64, 103, 195, 292, 309, 487, 982, 1103
MlyI GAGTC 3 cut(s) 45, 92, 659
MmeI TCCRAC 3 cut(s) 120, 618, 936
MnlI CCTC 8 cut(s) 36, 139, 189, 618, 622, 726, 945, 1112
Mph1103I ATGCAT 2 cut(s) 905, 947
MroXI GAANNNNTTC 1 cut(s) 1080
MseI TTAA 3 cut(s) 302, 963, 1160
MslI CAYNNNNRTG 3 cut(s) 254, 285, 434
MspI CCGG 3 cut(s) 17, 76, 1148
Mva1269I GAATGC 3 cut(s) 410, 843, 905
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeII GATC 4 cut(s) 208, 216, 548, 1234
NlaIII CATG 3 cut(s) 397, 949, 1022
NlaIV GGNNCC 1 cut(s) 177
NmuCI GTSAC 2 cut(s) 56, 511
NsiI ATGCAT 2 cut(s) 905, 947
NspV TTCGAA 1 cut(s) 738
PctI GAATGC 3 cut(s) 410, 843, 905
PdmI GAANNNNTTC 1 cut(s) 1080
PfeI GAWTC 3 cut(s) 89, 770, 1022
PkrI GCNGC 1 cut(s) 81
PleI GAGTC 3 cut(s) 45, 92, 659
PpsI GAGTC 3 cut(s) 45, 92, 659
PpuMI RGGWCCY 1 cut(s) 176
PsiI TTATAA 2 cut(s) 486, 749
Psp5II RGGWCCY 1 cut(s) 176
PspN4I GGNNCC 1 cut(s) 177
PspPI GGNCC 4 cut(s) 45, 176, 670, 1186
PspPPI RGGWCCY 1 cut(s) 176
PstI CTGCAG 1 cut(s) 507
PstNI CAGNNNCTG 3 cut(s) 54, 385, 1189
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 3 cut(s) 328, 1007, 1017
RsaNI GTAC 3 cut(s) 327, 1006, 1016
RseI CAYNNNNRTG 3 cut(s) 254, 285, 434
SaqAI TTAA 3 cut(s) 302, 963, 1160
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 4 cut(s) 208, 216, 548, 1234
Sau96I GGNCC 4 cut(s) 45, 176, 670, 1186
SchI GAGTC 3 cut(s) 45, 92, 659
SduI GDGCHC 2 cut(s) 645, 972
SfaNI GCATC 5 cut(s) 343, 368, 487, 533, 895
SfcI CTRYAG 2 cut(s) 503, 678
SfuI TTCGAA 1 cut(s) 738
SinI GGWCC 2 cut(s) 45, 176
SmiMI CAYNNNNRTG 3 cut(s) 254, 285, 434
SmlI CTYRAG 2 cut(s) 519, 790
SmoI CTYRAG 2 cut(s) 519, 790
Sse9I AATT 9 cut(s) 33, 64, 103, 195, 292, 309, 487, 982, 1103
SsiI CCGC 4 cut(s) 20, 80, 408, 546
SspI AATATT 2 cut(s) 766, 921
SspMI CTAG 1 cut(s) 315
TaaI ACNGT 2 cut(s) 256, 994
TaiI ACGT 1 cut(s) 1093
TaqI TCGA 4 cut(s) 131, 137, 577, 738
TasI AATT 9 cut(s) 33, 64, 103, 195, 292, 309, 487, 982, 1103
TatI WGTACW 1 cut(s) 1005
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 3 cut(s) 89, 770, 1022
Tru1I TTAA 3 cut(s) 302, 963, 1160
Tru9I TTAA 3 cut(s) 302, 963, 1160
TscAI CASTG 4 cut(s) 226, 261, 507, 516
TseFI GTSAC 2 cut(s) 56, 511
Tsp45I GTSAC 2 cut(s) 56, 511
TspDTI ATGAA 4 cut(s) 58, 962, 1013, 1160
TspGWI ACGGA 2 cut(s) 344, 794
TspRI CASTG 4 cut(s) 226, 261, 507, 516
VpaK11BI GGWCC 2 cut(s) 45, 176
XapI RAATTY 3 cut(s) 64, 309, 1103
XbaI TCTAGA 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 1080
XspI CTAG 1 cut(s) 315
Zsp2I ATGCAT 2 cut(s) 905, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.