Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006542

Physical Location & Seq

Genomic Coordinates Reverse (-)

2753305 .. 2757383

Gene Structure 4079 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1039000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGGGTGTTGTGGCCGAACATTCCGATCGCCAAACTATCAGAGATGTCATCTGCCACGCCGGTGCTGGCGCCTCCGCTGGTGCTATTGCGGCTACGTTTGTATGCCCCTTGGATGTCATCAAGACTAGGCTGCAGGTCCATGGCATGCCTTCTGGGCAAAGAGGTAGTATCATTGTCACAAGCCTACAAAAAATATTAAAGACTGAAGGTGTGAAGGGGATGTACCGGGGCCTTTCACCTACAATTCTAGCGCTACTTCCAAACTGGGCAGTGTACTTCACAGTTTATGAGCAACTCAAAGGCCTACTCCACACTCATGTTCATGGCATCGGAGAACTCACAATTGGTGAAAATATGATTGCTGCTGCTGGTGCCGGGGCTGCCACAGCCATTTCAACAAATCCATTGTGGGTTGTTAAGACCAGACTTCAAACACAAGGAATGAGGCCTGGTGTGATTCCTTACAAAAGCATGAGATCTGCTTTCTCAAGGATTGCAACTGAGGAAGGCTTGCGAGGTTTATATAGTGGGATCTTGCCTTCGTTGGCTGGCATAAGTCATGTTGCAATTCAATTTCCTGCTTATGAAAAGATAAAGTCTTTCATGGCAAAAAGGGATAATACAACTGTTGATAAGTTACACCCTAGTAGAGTTGCCTTTGCTTCATCAGTATCAAAAGTAATCGCTTCTGTAATAACATACCCTCACGAGGTTGTGCGCTCGAGGCTGCAAGAGCAAGGGCAGGCGAGACATATTGAACCCCAGTACGCAGGAGTTATTGACTGTATGAAGAAGGTGTTCCAAAAGGAAGGCCTTCGTGGATTCTATAATGGTTGTGCTACTAATCTACTAAGAACCACTCCTTCTGCCGTTATTACATTTACCAGCTACGAGATGATAGATAGGTTCTTGCGGAGGGTTGTACTTACAGAAAACGAGCACTCAGGTGGCCACCCTAAGTCTGATGGCCACGCCGAACCTCAGAAGGGAAATGGAGGCACCGAGAGAGAGGGGAATGACTCTGCATTGCGGCAAGCACAAGCCCAAACAAATCAGAGGACTCCACTTCCTCTTGGAAACAAAGACCCGCTAACGGCAAGACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006839 GO:0006862 GO:0008150 GO:0015215 GO:0015605 GO:0015748 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0019866 GO:0022857 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0035352 GO:0043132 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051181 GO:0051182 GO:0051184 GO:0051185 GO:0051234 GO:0051724 GO:0055085 GO:0071702 GO:0071705 GO:1901679

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mito_carr

Protein Analysis

369

Amino Acids

39.91

Weight (kDa)

9.71

Isoelectric Point (pI)

35.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mito_carr	PF00153	18 - 105	1.8e-26	Mitochondrial carrier protein
Mito_carr	PF00153	113 - 204	6.8e-23	Mitochondrial carrier protein
Mito_carr	PF00153	213 - 306	2e-23	Mitochondrial carrier protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25380 AT1G25380
fragaria_vesca	FvH4_4g26870 FvH4_4g26870 FvH4_4g26870
malus_domestica	MD13G1036400.v1.1 MD16G1039000.v1.1
prunus_persica	Prupe.1G315700_v2.0.a1 Prupe.1G315700_v2.0.a1 Prupe.1G315700_v2.0.a1 Prupe.1G315700_v2.0.a1 Prupe.1G315700_v2.0.a1 Prupe.1G315700_v2.0.a1
pyrus_communis	pycom13g03170 pycom16g03340
rosa_chinensis	RchiOBHm_Chr4g0434631
rosa_laevigata	RLG00000006661
rosa_multiflora	Rmu_co8445801.1_g000001 Rmu_sc0005783.1_g000002 Rmu_sc0005899.1_g000017
rosa_roxburghii	Rroxscaffold_5G00375870
rosa_rugosa	Rorug04G0277300
rosa_samantha	Rh4AG333200 Rh4BG341400 Rh4CG356200
rosa_wichuraiana	Rw4G028910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	124
AccB1I	GGYRCC	3 cut(s)	68, 371, 998
AciI	CCGC	5 cut(s)	75, 89, 913, 1030, 1088
AclWI	GGATC	1 cut(s)	539
AcoI	YGGCCR	3 cut(s)	12, 949, 967
AcuI	CTGAAG	1 cut(s)	225
AcyI	GRCGYC	1 cut(s)	69
AfaI	GTAC	4 cut(s)	224, 275, 767, 924
AfeI	AGCGCT	1 cut(s)	252
AfiI	CCNNNNNNNGG	3 cut(s)	709, 986, 1093
AgsI	TTSAA	4 cut(s)	396, 431, 572, 758
AjnI	CCWGG	1 cut(s)	448
AjuI	GAANNNNNNNTTGG	2 cut(s)	327, 359
AleI	CACNNNNGTG	2 cut(s)	60, 945
AluBI	AGCT	1 cut(s)	888
AluI	AGCT	1 cut(s)	888
Alw21I	GWGCWC	1 cut(s)	942
Alw26I	GTCTC	1 cut(s)	742
AlwI	GGATC	1 cut(s)	539
Ama87I	CYCGRG	1 cut(s)	721
Aor51HI	AGCGCT	1 cut(s)	252
AoxI	GGCC	7 cut(s)	12, 229, 301, 446, 811, 949, 967
ApeKI	GCWGC	5 cut(s)	130, 362, 365, 380, 727
Asp700I	GAANNNNTTC	2 cut(s)	797, 813
AspLEI	GCGC	3 cut(s)	71, 253, 720
AspS9I	GGNCC	2 cut(s)	136, 229
AsuC2I	CCSGG	2 cut(s)	227, 376
AsuHPI	GGTGA	2 cut(s)	228, 359
AvaI	CYCGRG	1 cut(s)	721
AvaII	GGWCC	1 cut(s)	136
BalI	TGGCCA	2 cut(s)	951, 969
BanI	GGYRCC	3 cut(s)	68, 371, 998
BarI	GAAGNNNNNNTAC	2 cut(s)	206, 238
BauI	CACGAG	1 cut(s)	707
Bbv12I	GWGCWC	1 cut(s)	942
BbvI	GCAGC	5 cut(s)	117, 349, 352, 367, 714
BccI	CCATC	1 cut(s)	959
BceAI	ACGGC	1 cut(s)	854
BciT130I	CCWGG	1 cut(s)	450
BcnI	CCSGG	2 cut(s)	227, 376
BcoDI	GTCTC	1 cut(s)	742
BfaI	CTAG	3 cut(s)	126, 248, 645
BfmI	CTRYAG	1 cut(s)	131
BfoI	RGCGCY	2 cut(s)	72, 254
BfuAI	ACCTGC	1 cut(s)	124
BglI	GCCNNNNNGGC	1 cut(s)	154
BglII	AGATCT	1 cut(s)	476
BisI	GCNGC	7 cut(s)	90, 131, 363, 366, 381, 728, 1031
BlsI	GCNGC	7 cut(s)	91, 132, 364, 367, 382, 729, 1032
Bme1390I	CCNGG	3 cut(s)	227, 376, 450
Bme18I	GGWCC	1 cut(s)	136
BmeT110I	CYCGRG	1 cut(s)	721
BmgT120I	GGNCC	2 cut(s)	136, 229
BmiI	GGNNCC	4 cut(s)	70, 230, 373, 1000
BmrFI	CCNGG	3 cut(s)	227, 376, 450
BmrI	ACTGGG	2 cut(s)	274, 757
BmsI	GCATC	1 cut(s)	336
BmuI	ACTGGG	2 cut(s)	274, 757
BpuEI	CTTGAG	1 cut(s)	472
BpuMI	CCSGG	2 cut(s)	227, 376
BsaHI	GRCGYC	1 cut(s)	69
BsaJI	CCNNGG	4 cut(s)	108, 139, 226, 375
Bsc4I	CCNNNNNNNGG	3 cut(s)	709, 986, 1093
Bse118I	RCCGGY	1 cut(s)	59
Bse1I	ACTGG	2 cut(s)	269, 763
Bse3DI	GCAATG	1 cut(s)	1025
BseBI	CCWGG	1 cut(s)	450
BseDI	CCNNGG	4 cut(s)	108, 139, 226, 375
BseGI	GGATG	2 cut(s)	118, 225
BseLI	CCNNNNNNNGG	3 cut(s)	709, 986, 1093
BseMI	GCAATG	1 cut(s)	1025
BseMII	CTCAG	3 cut(s)	492, 957, 995
BseNI	ACTGG	2 cut(s)	269, 763
BseXI	GCAGC	5 cut(s)	117, 349, 352, 367, 714
Bsh1285I	CGRYCG	1 cut(s)	28
BshFI	GGCC	7 cut(s)	14, 231, 303, 448, 813, 951, 969
BshNI	GGYRCC	3 cut(s)	68, 371, 998
BsiEI	CGRYCG	1 cut(s)	28
BsiHKAI	GWGCWC	1 cut(s)	942
BsiHKCI	CYCGRG	1 cut(s)	721
BsiSI	CCGG	3 cut(s)	60, 226, 375
BslI	CCNNNNNNNGG	3 cut(s)	709, 986, 1093
BsmAI	GTCTC	1 cut(s)	742
BsnI	GGCC	7 cut(s)	14, 231, 303, 448, 813, 951, 969
BsoBI	CYCGRG	1 cut(s)	721
Bsp1286I	GDGCHC	1 cut(s)	942
Bsp143I	GATC	3 cut(s)	25, 476, 531
Bsp19I	CCATGG	1 cut(s)	139
BspACI	CCGC	5 cut(s)	75, 89, 913, 1030, 1088
BspANI	GGCC	7 cut(s)	14, 231, 303, 448, 813, 951, 969
BspCNI	CTCAG	3 cut(s)	493, 956, 994
BspLI	GGNNCC	4 cut(s)	70, 230, 373, 1000
BspMAI	CTGCAG	1 cut(s)	135
BspMI	ACCTGC	1 cut(s)	124
BspPI	GGATC	1 cut(s)	539
BspT107I	GGYRCC	3 cut(s)	68, 371, 998
BsrDI	GCAATG	1 cut(s)	1025
BsrFI	RCCGGY	1 cut(s)	59
BsrI	ACTGG	2 cut(s)	269, 763
BssAI	RCCGGY	1 cut(s)	59
BssECI	CCNNGG	4 cut(s)	108, 139, 226, 375
BssMI	GATC	3 cut(s)	25, 476, 531
BssNI	GRCGYC	1 cut(s)	69
BssSI	CACGAG	1 cut(s)	707
BssT1I	CCWWGG	2 cut(s)	108, 139
Bst2BI	CACGAG	1 cut(s)	707
Bst2UI	CCWGG	1 cut(s)	450
Bst4CI	ACNGT	3 cut(s)	283, 628, 785
BstACI	GRCGYC	1 cut(s)	69
BstC8I	GCNNGC	6 cut(s)	67, 146, 512, 550, 744, 1035
BstDEI	CTNAG	5 cut(s)	501, 851, 943, 957, 981
BstDSI	CCRYGG	1 cut(s)	139
BstF5I	GGATG	2 cut(s)	118, 225
BstH2I	RGCGCY	2 cut(s)	72, 254
BstHHI	GCGC	3 cut(s)	71, 253, 720
BstKTI	GATC	3 cut(s)	28, 479, 534
BstMAI	GTCTC	1 cut(s)	742
BstMBI	GATC	3 cut(s)	25, 476, 531
BstMCI	CGRYCG	1 cut(s)	28
BstMWI	GCNNNNNNNGC	7 cut(s)	89, 154, 371, 380, 386, 724, 733
BstNI	CCWGG	1 cut(s)	450
BstNSI	RCATGY	1 cut(s)	148
BstSCI	CCNGG	3 cut(s)	225, 374, 448
BstSFI	CTRYAG	1 cut(s)	131
BstV1I	GCAGC	5 cut(s)	117, 349, 352, 367, 714
BstX2I	RGATCY	2 cut(s)	476, 531
BstYI	RGATCY	2 cut(s)	476, 531
BsuRI	GGCC	7 cut(s)	14, 231, 303, 448, 813, 951, 969
BtgI	CCRYGG	1 cut(s)	139
BtsCI	GGATG	2 cut(s)	118, 225
BtsI	GCAGTG	1 cut(s)	276
BtsIMutI	CAGTG	1 cut(s)	276
BveI	ACCTGC	1 cut(s)	124
Cac8I	GCNNGC	6 cut(s)	67, 146, 512, 550, 744, 1035
CfoI	GCGC	3 cut(s)	71, 253, 720
Cfr10I	RCCGGY	1 cut(s)	59
Cfr13I	GGNCC	2 cut(s)	136, 229
Csp6I	GTAC	4 cut(s)	223, 274, 766, 923
CviAII	CATG	7 cut(s)	140, 145, 317, 323, 472, 560, 604
CviQI	GTAC	4 cut(s)	223, 274, 766, 923
DdeI	CTNAG	5 cut(s)	501, 851, 943, 957, 981
DinI	GGCGCC	1 cut(s)	70
DpnI	GATC	3 cut(s)	27, 478, 533
DpnII	GATC	3 cut(s)	25, 476, 531
EaeI	YGGCCR	3 cut(s)	12, 949, 967
Eco130I	CCWWGG	2 cut(s)	108, 139
Eco147I	AGGCCT	3 cut(s)	303, 448, 813
Eco47I	GGWCC	1 cut(s)	136
Eco47III	AGCGCT	1 cut(s)	252
Eco57I	CTGAAG	1 cut(s)	225
Eco88I	CYCGRG	1 cut(s)	721
EcoO109I	RGGNCCY	1 cut(s)	229
EcoRII	CCWGG	1 cut(s)	448
EcoT14I	CCWWGG	2 cut(s)	108, 139
EgeI	GGCGCC	1 cut(s)	70
EheI	GGCGCC	1 cut(s)	70
ErhI	CCWWGG	2 cut(s)	108, 139
FaeI	CATG	7 cut(s)	143, 148, 320, 326, 475, 563, 607
FatI	CATG	7 cut(s)	139, 144, 316, 322, 471, 559, 603
FauI	CCCGC	1 cut(s)	1095
Fnu4HI	GCNGC	7 cut(s)	90, 131, 363, 366, 381, 728, 1031
FokI	GGATG	2 cut(s)	125, 232
Fsp4HI	GCNGC	7 cut(s)	90, 131, 363, 366, 381, 728, 1031
FspBI	CTAG	3 cut(s)	126, 248, 645
GlaI	GCGC	3 cut(s)	70, 252, 719
GluI	GCNGC	7 cut(s)	90, 131, 363, 366, 381, 728, 1031
HaeII	RGCGCY	2 cut(s)	72, 254
HaeIII	GGCC	7 cut(s)	14, 231, 303, 448, 813, 951, 969
HapII	CCGG	3 cut(s)	60, 226, 375
HhaI	GCGC	3 cut(s)	71, 253, 720
Hin1I	GRCGYC	1 cut(s)	69
Hin1II	CATG	7 cut(s)	143, 148, 320, 326, 475, 563, 607
Hin6I	GCGC	3 cut(s)	69, 251, 718
HinP1I	GCGC	3 cut(s)	69, 251, 718
HinfI	GANTC	4 cut(s)	457, 822, 1019, 1060
HpaII	CCGG	3 cut(s)	60, 226, 375
HphI	GGTGA	2 cut(s)	228, 359
Hpy166II	GTNNAC	1 cut(s)	274
Hpy188I	TCNGA	6 cut(s)	25, 41, 332, 964, 984, 1056
Hpy188III	TCNNGA	2 cut(s)	121, 707
Hpy8I	GTNNAC	1 cut(s)	274
HpyCH4III	ACNGT	3 cut(s)	283, 628, 785
HpyCH4IV	ACGT	1 cut(s)	95
HpyCH4V	TGCA	5 cut(s)	133, 497, 566, 730, 1025
HpyF10VI	GCNNNNNNNGC	7 cut(s)	89, 154, 371, 380, 386, 724, 733
HpyF3I	CTNAG	5 cut(s)	501, 851, 943, 957, 981
HpySE526I	ACGT	1 cut(s)	95
Hsp92I	GRCGYC	1 cut(s)	69
Hsp92II	CATG	7 cut(s)	143, 148, 320, 326, 475, 563, 607
HspAI	GCGC	3 cut(s)	69, 251, 718
KasI	GGCGCC	1 cut(s)	68
Kzo9I	GATC	3 cut(s)	25, 476, 531
Lsp1109I	GCAGC	5 cut(s)	117, 349, 352, 367, 714
LweI	GCATC	1 cut(s)	336
MaeI	CTAG	3 cut(s)	126, 248, 645
MaeII	ACGT	1 cut(s)	95
MaeIII	GTNAC	2 cut(s)	175, 636
MalI	GATC	3 cut(s)	27, 478, 533
MboI	GATC	3 cut(s)	25, 476, 531
MboII	GAAGA	1 cut(s)	802
MfeI	CAATTG	1 cut(s)	342
MflI	RGATCY	2 cut(s)	476, 531
MhlI	GDGCHC	1 cut(s)	942
MlsI	TGGCCA	2 cut(s)	951, 969
MluCI	AATT	4 cut(s)	243, 342, 567, 572
MluNI	TGGCCA	2 cut(s)	951, 969
Mly113I	GGCGCC	1 cut(s)	69
MlyI	GAGTC	2 cut(s)	1013, 1054
Mox20I	TGGCCA	2 cut(s)	951, 969
MroXI	GAANNNNTTC	2 cut(s)	797, 813
MscI	TGGCCA	2 cut(s)	951, 969
MseI	TTAA	2 cut(s)	197, 417
MslI	CAYNNNNRTG	4 cut(s)	60, 315, 321, 945
Msp20I	TGGCCA	2 cut(s)	951, 969
MspA1I	CMGCKG	1 cut(s)	77
MspI	CCGG	3 cut(s)	60, 226, 375
MspR9I	CCNGG	3 cut(s)	227, 376, 450
MunI	CAATTG	1 cut(s)	342
MvaI	CCWGG	1 cut(s)	450
MwoI	GCNNNNNNNGC	7 cut(s)	89, 154, 371, 380, 386, 724, 733
NarI	GGCGCC	1 cut(s)	69
NciI	CCSGG	2 cut(s)	227, 376
NcoI	CCATGG	1 cut(s)	139
NdeII	GATC	3 cut(s)	25, 476, 531
NlaIII	CATG	7 cut(s)	143, 148, 320, 326, 475, 563, 607
NlaIV	GGNNCC	4 cut(s)	70, 230, 373, 1000
NmuCI	GTSAC	1 cut(s)	175
NspI	RCATGY	1 cut(s)	148
OliI	CACNNNNGTG	2 cut(s)	60, 945
PaeI	GCATGC	1 cut(s)	148
PaeR7I	CTCGAG	1 cut(s)	721
PceI	AGGCCT	3 cut(s)	303, 448, 813
PdmI	GAANNNNTTC	2 cut(s)	797, 813
PfeI	GAWTC	2 cut(s)	457, 822
PkrI	GCNGC	7 cut(s)	91, 132, 364, 367, 382, 729, 1032
Ple19I	CGATCG	1 cut(s)	28
PleI	GAGTC	2 cut(s)	1013, 1054
PluTI	GGCGCC	1 cut(s)	72
PpsI	GAGTC	2 cut(s)	1013, 1054
Psp6I	CCWGG	1 cut(s)	448
PspGI	CCWGG	1 cut(s)	448
PspN4I	GGNNCC	4 cut(s)	70, 230, 373, 1000
PspPI	GGNCC	2 cut(s)	136, 229
PspXI	VCTCGAGB	1 cut(s)	721
PstI	CTGCAG	1 cut(s)	135
PsuI	RGATCY	2 cut(s)	476, 531
PvuI	CGATCG	1 cut(s)	28
RsaI	GTAC	4 cut(s)	224, 275, 767, 924
RsaNI	GTAC	4 cut(s)	223, 274, 766, 923
RseI	CAYNNNNRTG	4 cut(s)	60, 315, 321, 945
SaqAI	TTAA	2 cut(s)	197, 417
SatI	GCNGC	7 cut(s)	90, 131, 363, 366, 381, 728, 1031
Sau3AI	GATC	3 cut(s)	25, 476, 531
Sau96I	GGNCC	2 cut(s)	136, 229
SchI	GAGTC	2 cut(s)	1013, 1054
ScrFI	CCNGG	3 cut(s)	227, 376, 450
SduI	GDGCHC	1 cut(s)	942
SfaNI	GCATC	1 cut(s)	336
SfcI	CTRYAG	1 cut(s)	131
SfoI	GGCGCC	1 cut(s)	70
Sfr274I	CTCGAG	1 cut(s)	721
SgrAI	CRCCGGYG	1 cut(s)	59
SinI	GGWCC	1 cut(s)	136
SlaI	CTCGAG	1 cut(s)	721
SmiMI	CAYNNNNRTG	4 cut(s)	60, 315, 321, 945
SmlI	CTYRAG	2 cut(s)	487, 721
SmoI	CTYRAG	2 cut(s)	487, 721
SphI	GCATGC	1 cut(s)	148
Sse9I	AATT	4 cut(s)	243, 342, 567, 572
SseBI	AGGCCT	3 cut(s)	303, 448, 813
SsiI	CCGC	5 cut(s)	75, 89, 913, 1030, 1088
SspDI	GGCGCC	1 cut(s)	68
SspI	AATATT	1 cut(s)	195
SspMI	CTAG	3 cut(s)	126, 248, 645
StuI	AGGCCT	3 cut(s)	303, 448, 813
StyD4I	CCNGG	3 cut(s)	225, 374, 448
StyI	CCWWGG	2 cut(s)	108, 139
TaaI	ACNGT	3 cut(s)	283, 628, 785
TaiI	ACGT	1 cut(s)	98
TaqI	TCGA	1 cut(s)	722
TasI	AATT	4 cut(s)	243, 342, 567, 572
TatI	WGTACW	2 cut(s)	273, 922
TauI	GCSGC	2 cut(s)	92, 1033
TfiI	GAWTC	2 cut(s)	457, 822
Tru1I	TTAA	2 cut(s)	197, 417
Tru9I	TTAA	2 cut(s)	197, 417
TscAI	CASTG	1 cut(s)	276
TseFI	GTSAC	1 cut(s)	175
TseI	GCWGC	5 cut(s)	130, 362, 365, 380, 727
Tsp45I	GTSAC	1 cut(s)	175
TspDTI	ATGAA	5 cut(s)	311, 592, 600, 654, 803
TspRI	CASTG	1 cut(s)	276
VpaK11BI	GGWCC	1 cut(s)	136
XceI	RCATGY	1 cut(s)	148
XcmI	CCANNNNNNNNNTGG	1 cut(s)	62
XhoI	CTCGAG	1 cut(s)	721
XmnI	GAANNNNTTC	2 cut(s)	797, 813
XspI	CTAG	3 cut(s)	126, 248, 645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.