Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011639

Physical Location & Seq

Genomic Coordinates Reverse (-)

3266698 .. 3269842

Gene Structure 3145 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1047100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGGTTACAACTTCTCCTCCTCCAAAGGAAGTTGTTTCAGACGACAAATGGAAGGAGGAGCCGGTGGACAACGCCCGCCGAGCAAAATGGTGGTACTCCACCTTTCACACAGTCACCGCCATGATCGGTGCCGGTGTACTCAGCCTGCCGTATGCCATGGCCTTCTTAGGATGGGGTCCAGGGACAATGGTGCTGGTAATATCATGGTGCATGACCTTGAACACAATGTGGCAGATGATTCAGCTCCATGAATGTGTTCCGGGAACTAGGTTTGACCGATACATCGACCTTGGCCGACACGCCTTCGGACCAAAGTTCGGGCCATGGATTGTGCTTCCGCAACAAATGATTGTGCAGGTTGGTTGTGACATAGTGTACATGGTCACCGGAGGAAAGTGTCTAAAGAAATTTGTGGAGATGGCATGTGCCAATTGCACACCAGTCAAGCAATCGTACTGGATTGTCATCTTTGGTTCCCTTCATTTCTTTCTTTCCCAGCTCCCCAATTTCAACTCTGTTGCTGGTGTTTCTCTAGCAGCAGCTATCATGTCACTAAGTTATTCAACTATAGCGTGGGCAGGTTGCTTAAGCAAAGGTCAGGTTGAAAACGTGAGCTATGCATACAAGAAAACAAGCCCTGCAGATTACATGTTTAGGGTTTTCAATGCATTAGGTCAAATCTCGTTTGCATTTGCTGGGCATGCAGTGGTGCTTGAAATCCAAGCCACAATTCCATCGACGCCTGAGAAGCCGTCGAGAATTCCCATGTGGAAAGGTGCTCTAGGAGCCTACTTTATCAACGCAATTTGCTATTTCCCGGTGGCCCTCATCGGGTACTGGGCTTTTGGTCAAGATGTCGATGATAACGTGCTCATAGATCTTAAAAAACCTACATGGCTCATTGCTGCTGCTAACCTAATGGTTGTCGTCCATGTCATTGGCAGCTACCAGGTTTATGCTATGCCTGTTTTTGACATGCTTGAGAGGATGATGGTGAAAAAGATGAACTTCCCACCTGGAATTGCACTTAGACTAATCACGAGATCAGCTTATGTTGCATTTACATTATTTGTTGGAGTCACCTTCCCTTTCTTTGGAGACCTTCTTGGTTTCTTTGGTGGATTTGGTTTTGCCCCCACTTCATACTTTCTTCCTTGTATAATGTGGCTGAGAATTAAGAAACCAAAGAGATACAGCATTCAATGGCTCGTCAACTGGGTTTGCATATTCATCGGAGTGTTCATCATGTTGGCTTCCACAGTTGGAGGTTTTCGTAATATCATTGCTGATGCATCAACATATCGTTTTTACACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0034220 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

439

Amino Acids

49.1

Weight (kDa)

9.16

Isoelectric Point (pI)

34.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	27 - 422	1.5e-102	Transmembrane amino acid transporter protein
Trp_Tyr_perm	PF03222	29 - 159	2.8e-07	Tryptophan/tyrosine permease family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011639)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25530
fragaria_vesca	FvH4_4g27830 FvH4_4g27960 FvH4_4g27960 FvH4_4g27960 FvH4_4g27960
malus_domestica	MD13G1045300.v1.1 MD16G1046600.v1.1 MD16G1047100.v1.1
prunus_persica	Prupe.1G306700_v2.0.a1 Prupe.1G306700_v2.0.a1
pyrus_communis	pycom13g03990 pycom16g04100
rosa_chinensis	RchiOBHm_Chr4g0435661
rosa_laevigata	RLG00000006569
rosa_multiflora	Rmu_sc0000051.1_g000010
rosa_rugosa	Rorug04G0287800
rosa_samantha	Rh4AG342900
rosa_wichuraiana	Rw4G029930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	346, 569
AccB1I	GGYRCC	1 cut(s)	128
AciI	CCGC	3 cut(s)	76, 117, 338
AcoI	YGGCCR	1 cut(s)	292
AcsI	RAATTY	2 cut(s)	407, 759
AcyI	GRCGYC	1 cut(s)	740
AdeI	CACNNNGTG	1 cut(s)	228
AfaI	GTAC	5 cut(s)	95, 138, 377, 455, 836
AfiI	CCNNNNNNNGG	3 cut(s)	317, 831, 1094
AflII	CTTAAG	1 cut(s)	586
AflIII	ACRYGT	2 cut(s)	648, 1311
AgsI	TTSAA	7 cut(s)	220, 511, 564, 605, 664, 716, 1202
AjnI	CCWGG	3 cut(s)	178, 948, 1015
AjuI	GAANNNNNNNTTGG	2 cut(s)	714, 746
AluBI	AGCT	6 cut(s)	244, 499, 542, 615, 945, 1049
AluI	AGCT	6 cut(s)	244, 499, 542, 615, 945, 1049
Alw21I	GWGCWC	2 cut(s)	781, 873
Alw26I	GTCTC	1 cut(s)	1092
AoxI	GGCC	4 cut(s)	159, 292, 320, 822
ApeKI	GCWGC	5 cut(s)	536, 539, 905, 908, 942
ApoI	RAATTY	2 cut(s)	407, 759
Asp700I	GAANNNNTTC	1 cut(s)	255
AspS9I	GGNCC	4 cut(s)	176, 308, 320, 823
AsuC2I	CCSGG	2 cut(s)	261, 818
AsuHPI	GGTGA	4 cut(s)	106, 376, 1006, 1072
AvaII	GGWCC	2 cut(s)	176, 308
BanI	GGYRCC	1 cut(s)	128
BauI	CACGAG	1 cut(s)	1039
Bbv12I	GWGCWC	2 cut(s)	781, 873
BbvI	GCAGC	5 cut(s)	548, 551, 892, 895, 954
BccI	CCATC	4 cut(s)	165, 412, 742, 985
BceAI	ACGGC	2 cut(s)	133, 736
BcgI	CGANNNNNNTGC	2 cut(s)	1213, 1247
BciT130I	CCWGG	3 cut(s)	180, 950, 1017
BcnI	CCSGG	2 cut(s)	261, 818
BcoDI	GTCTC	1 cut(s)	1092
BfaI	CTAG	3 cut(s)	267, 533, 782
BfmI	CTRYAG	2 cut(s)	567, 639
BfrI	CTTAAG	1 cut(s)	586
BfuAI	ACCTGC	2 cut(s)	346, 569
BglII	AGATCT	1 cut(s)	877
BisI	GCNGC	5 cut(s)	537, 540, 906, 909, 943
BlsI	GCNGC	5 cut(s)	538, 541, 907, 910, 944
Bme1390I	CCNGG	5 cut(s)	180, 261, 818, 950, 1017
Bme18I	GGWCC	2 cut(s)	176, 308
BmgT120I	GGNCC	4 cut(s)	176, 308, 320, 823
BmiI	GGNNCC	5 cut(s)	60, 130, 177, 475, 787
BmrFI	CCNGG	5 cut(s)	180, 261, 818, 950, 1017
BmrI	ACTGGG	2 cut(s)	847, 1225
BmsI	GCATC	2 cut(s)	1279, 1301
BmuI	ACTGGG	2 cut(s)	847, 1225
BpuEI	CTTGAG	1 cut(s)	1001
BpuMI	CCSGG	2 cut(s)	261, 818
BsaAI	YACGTR	1 cut(s)	1314
BsaBI	GATNNNNATC	1 cut(s)	464
BsaHI	GRCGYC	1 cut(s)	740
BsaI	GGTCTC	1 cut(s)	1092
BsaJI	CCNNGG	4 cut(s)	156, 179, 289, 323
BsaWI	WCCGGW	1 cut(s)	386
BsaXI	ACNNNNNCTCC	2 cut(s)	28, 31
Bsc4I	CCNNNNNNNGG	3 cut(s)	317, 831, 1094
Bse118I	RCCGGY	2 cut(s)	61, 131
Bse1I	ACTGG	4 cut(s)	440, 461, 842, 1220
Bse3DI	GCAATG	2 cut(s)	900, 1281
Bse8I	GATNNNNATC	1 cut(s)	464
BseBI	CCWGG	3 cut(s)	180, 950, 1017
BseDI	CCNNGG	4 cut(s)	156, 179, 289, 323
BseGI	GGATG	2 cut(s)	176, 993
BseJI	GATNNNNATC	1 cut(s)	464
BseLI	CCNNNNNNNGG	3 cut(s)	317, 831, 1094
BseMI	GCAATG	2 cut(s)	900, 1281
BseMII	CTCAG	3 cut(s)	154, 735, 1160
BseNI	ACTGG	4 cut(s)	440, 461, 842, 1220
BseRI	GAGGAG	3 cut(s)	6, 9, 71
BseXI	GCAGC	5 cut(s)	548, 551, 892, 895, 954
BseYI	CCCAGC	2 cut(s)	495, 695
BsgI	GTGCAG	1 cut(s)	374
BshFI	GGCC	4 cut(s)	161, 294, 322, 824
BshNI	GGYRCC	1 cut(s)	128
BsiHKAI	GWGCWC	2 cut(s)	781, 873
BsiSI	CCGG	5 cut(s)	62, 132, 260, 387, 818
BslFI	GGGAC	1 cut(s)	196
BslI	CCNNNNNNNGG	3 cut(s)	317, 831, 1094
BsmAI	GTCTC	1 cut(s)	1092
BsmFI	GGGAC	1 cut(s)	196
BsmI	GAATGC	1 cut(s)	1197
BsnI	GGCC	4 cut(s)	161, 294, 322, 824
Bso31I	GGTCTC	1 cut(s)	1092
Bsp1286I	GDGCHC	2 cut(s)	781, 873
Bsp1407I	TGTACA	1 cut(s)	375
Bsp143I	GATC	3 cut(s)	123, 877, 1043
Bsp19I	CCATGG	2 cut(s)	156, 323
BspACI	CCGC	3 cut(s)	76, 117, 338
BspANI	GGCC	4 cut(s)	161, 294, 322, 824
BspCNI	CTCAG	3 cut(s)	153, 736, 1161
BspLI	GGNNCC	5 cut(s)	60, 130, 177, 475, 787
BspMAI	CTGCAG	1 cut(s)	643
BspMI	ACCTGC	2 cut(s)	346, 569
BspT107I	GGYRCC	1 cut(s)	128
BspTI	CTTAAG	1 cut(s)	586
BspTNI	GGTCTC	1 cut(s)	1092
BsrDI	GCAATG	2 cut(s)	900, 1281
BsrFI	RCCGGY	2 cut(s)	61, 131
BsrGI	TGTACA	1 cut(s)	375
BsrI	ACTGG	4 cut(s)	440, 461, 842, 1220
BssAI	RCCGGY	2 cut(s)	61, 131
BssECI	CCNNGG	4 cut(s)	156, 179, 289, 323
BssMI	GATC	3 cut(s)	123, 877, 1043
BssNI	GRCGYC	1 cut(s)	740
BssSI	CACGAG	1 cut(s)	1039
BssT1I	CCWWGG	3 cut(s)	156, 289, 323
Bst2BI	CACGAG	1 cut(s)	1039
Bst2UI	CCWGG	3 cut(s)	180, 950, 1017
Bst4CI	ACNGT	2 cut(s)	112, 1261
BstACI	GRCGYC	1 cut(s)	740
BstAFI	CTTAAG	1 cut(s)	586
BstAUI	TGTACA	1 cut(s)	375
BstBAI	YACGTR	1 cut(s)	1314
BstC8I	GCNNGC	3 cut(s)	76, 146, 702
BstDEI	CTNAG	6 cut(s)	140, 166, 554, 744, 1028, 1169
BstDSI	CCRYGG	2 cut(s)	156, 323
BstEII	GGTNACC	1 cut(s)	382
BstF5I	GGATG	2 cut(s)	176, 993
BstKTI	GATC	3 cut(s)	126, 880, 1046
BstMAI	GTCTC	1 cut(s)	1092
BstMBI	GATC	3 cut(s)	123, 877, 1043
BstMWI	GCNNNNNNNGC	5 cut(s)	80, 701, 748, 785, 1055
BstNI	CCWGG	3 cut(s)	180, 950, 1017
BstNSI	RCATGY	4 cut(s)	426, 652, 704, 979
BstPI	GGTNACC	1 cut(s)	382
BstSCI	CCNGG	5 cut(s)	178, 259, 816, 948, 1015
BstSFI	CTRYAG	2 cut(s)	567, 639
BstV1I	GCAGC	5 cut(s)	548, 551, 892, 895, 954
BstX2I	RGATCY	1 cut(s)	877
BstXI	CCANNNNNNTGG	1 cut(s)	938
BstYI	RGATCY	1 cut(s)	877
BsuRI	GGCC	4 cut(s)	161, 294, 322, 824
BtgI	CCRYGG	2 cut(s)	156, 323
BtsCI	GGATG	2 cut(s)	176, 993
BtsI	GCAGTG	1 cut(s)	711
BtsIMutI	CAGTG	1 cut(s)	711
BveI	ACCTGC	2 cut(s)	346, 569
Cac8I	GCNNGC	3 cut(s)	76, 146, 702
Cfr10I	RCCGGY	2 cut(s)	61, 131
Cfr13I	GGNCC	4 cut(s)	176, 308, 320, 823
CseI	GACGC	1 cut(s)	748
CsiI	ACCWGGT	1 cut(s)	948
Csp6I	GTAC	5 cut(s)	94, 137, 376, 454, 835
CviQI	GTAC	5 cut(s)	94, 137, 376, 454, 835
DdeI	CTNAG	6 cut(s)	140, 166, 554, 744, 1028, 1169
DpnI	GATC	3 cut(s)	125, 879, 1045
DpnII	GATC	3 cut(s)	123, 877, 1043
DraIII	CACNNNGTG	1 cut(s)	228
EaeI	YGGCCR	1 cut(s)	292
Eco130I	CCWWGG	3 cut(s)	156, 289, 323
Eco31I	GGTCTC	1 cut(s)	1092
Eco47I	GGWCC	2 cut(s)	176, 308
Eco91I	GGTNACC	1 cut(s)	382
EcoO65I	GGTNACC	1 cut(s)	382
EcoRI	GAATTC	1 cut(s)	759
EcoRII	CCWGG	3 cut(s)	178, 948, 1015
EcoT14I	CCWWGG	3 cut(s)	156, 289, 323
EcoT22I	ATGCAT	3 cut(s)	622, 670, 1294
ErhI	CCWWGG	3 cut(s)	156, 289, 323
FalI	AAGNNNNNCTT	2 cut(s)	992, 1024
FaqI	GGGAC	1 cut(s)	196
FauI	CCCGC	1 cut(s)	83
Fnu4HI	GCNGC	5 cut(s)	537, 540, 906, 909, 943
FokI	GGATG	2 cut(s)	183, 1000
Fsp4HI	GCNGC	5 cut(s)	537, 540, 906, 909, 943
FspBI	CTAG	3 cut(s)	267, 533, 782
GluI	GCNGC	5 cut(s)	537, 540, 906, 909, 943
GsaI	CCCAGC	2 cut(s)	499, 699
HaeIII	GGCC	4 cut(s)	161, 294, 322, 824
HapII	CCGG	5 cut(s)	62, 132, 260, 387, 818
HgaI	GACGC	1 cut(s)	748
Hin1I	GRCGYC	1 cut(s)	740
HincII	GTYRAC	1 cut(s)	1213
HindII	GTYRAC	1 cut(s)	1213
HinfI	GANTC	2 cut(s)	238, 1077
HpaII	CCGG	5 cut(s)	62, 132, 260, 387, 818
HphI	GGTGA	4 cut(s)	106, 376, 1006, 1072
Hpy166II	GTNNAC	4 cut(s)	67, 137, 376, 1213
Hpy188I	TCNGA	3 cut(s)	40, 308, 1235
Hpy188III	TCNNGA	3 cut(s)	756, 851, 1039
Hpy8I	GTNNAC	4 cut(s)	67, 137, 376, 1213
Hpy99I	CGWCG	2 cut(s)	742, 757
HpyAV	CCTTC	6 cut(s)	46, 172, 313, 488, 1093, 1112
HpyCH4III	ACNGT	2 cut(s)	112, 1261
HpyCH4IV	ACGT	3 cut(s)	609, 867, 1313
HpyF10VI	GCNNNNNNNGC	5 cut(s)	80, 701, 748, 785, 1055
HpyF3I	CTNAG	6 cut(s)	140, 166, 554, 744, 1028, 1169
HpySE526I	ACGT	3 cut(s)	609, 867, 1313
Hsp92I	GRCGYC	1 cut(s)	740
Kzo9I	GATC	3 cut(s)	123, 877, 1043
LmnI	GCTCC	4 cut(s)	58, 249, 504, 785
Lsp1109I	GCAGC	5 cut(s)	548, 551, 892, 895, 954
LweI	GCATC	2 cut(s)	1279, 1301
MabI	ACCWGGT	1 cut(s)	948
MaeI	CTAG	3 cut(s)	267, 533, 782
MaeII	ACGT	3 cut(s)	609, 867, 1313
MaeIII	GTNAC	6 cut(s)	4, 112, 365, 382, 549, 1078
MalI	GATC	3 cut(s)	125, 879, 1045
MboI	GATC	3 cut(s)	123, 877, 1043
MboII	GAAGA	1 cut(s)	1142
MfeI	CAATTG	1 cut(s)	430
MflI	RGATCY	1 cut(s)	877
MhlI	GDGCHC	2 cut(s)	781, 873
MluCI	AATT	8 cut(s)	407, 430, 505, 729, 759, 804, 1020, 1173
MlyI	GAGTC	1 cut(s)	1086
MmeI	TCCRAC	2 cut(s)	1054, 1243
MnlI	CCTC	7 cut(s)	27, 30, 49, 383, 836, 978, 1259
Mph1103I	ATGCAT	3 cut(s)	622, 670, 1294
MroXI	GAANNNNTTC	1 cut(s)	255
MseI	TTAA	3 cut(s)	587, 882, 1176
MslI	CAYNNNNRTG	3 cut(s)	119, 252, 1235
MspCI	CTTAAG	1 cut(s)	586
MspI	CCGG	5 cut(s)	62, 132, 260, 387, 818
MspR9I	CCNGG	5 cut(s)	180, 261, 818, 950, 1017
MunI	CAATTG	1 cut(s)	430
Mva1269I	GAATGC	1 cut(s)	1197
MvaI	CCWGG	3 cut(s)	180, 950, 1017
MwoI	GCNNNNNNNGC	5 cut(s)	80, 701, 748, 785, 1055
NciI	CCSGG	2 cut(s)	261, 818
NcoI	CCATGG	2 cut(s)	156, 323
NdeII	GATC	3 cut(s)	123, 877, 1043
NlaIV	GGNNCC	5 cut(s)	60, 130, 177, 475, 787
NmeAIII	GCCGAG	1 cut(s)	104
NmuCI	GTSAC	5 cut(s)	112, 365, 382, 549, 1078
NsiI	ATGCAT	3 cut(s)	622, 670, 1294
NspI	RCATGY	4 cut(s)	426, 652, 704, 979
PaeI	GCATGC	1 cut(s)	704
PciI	ACATGT	1 cut(s)	648
PcsI	WCGNNNNNNNCGW	1 cut(s)	864
PctI	GAATGC	1 cut(s)	1197
PdmI	GAANNNNTTC	1 cut(s)	255
PfeI	GAWTC	1 cut(s)	238
PfoI	TCCNGGA	1 cut(s)	259
PkrI	GCNGC	5 cut(s)	538, 541, 907, 910, 944
PleI	GAGTC	1 cut(s)	1085
PpsI	GAGTC	1 cut(s)	1085
Ppu21I	YACGTR	1 cut(s)	1314
PscI	ACATGT	1 cut(s)	648
Psp6I	CCWGG	3 cut(s)	178, 948, 1015
PspEI	GGTNACC	1 cut(s)	382
PspFI	CCCAGC	2 cut(s)	495, 695
PspGI	CCWGG	3 cut(s)	178, 948, 1015
PspN4I	GGNNCC	5 cut(s)	60, 130, 177, 475, 787
PspPI	GGNCC	4 cut(s)	176, 308, 320, 823
PstI	CTGCAG	1 cut(s)	643
PsuI	RGATCY	1 cut(s)	877
RsaI	GTAC	5 cut(s)	95, 138, 377, 455, 836
RsaNI	GTAC	5 cut(s)	94, 137, 376, 454, 835
RseI	CAYNNNNRTG	3 cut(s)	119, 252, 1235
SaqAI	TTAA	3 cut(s)	587, 882, 1176
SatI	GCNGC	5 cut(s)	537, 540, 906, 909, 943
Sau3AI	GATC	3 cut(s)	123, 877, 1043
Sau96I	GGNCC	4 cut(s)	176, 308, 320, 823
SchI	GAGTC	1 cut(s)	1086
ScrFI	CCNGG	5 cut(s)	180, 261, 818, 950, 1017
SduI	GDGCHC	2 cut(s)	781, 873
SexAI	ACCWGGT	1 cut(s)	948
SfaNI	GCATC	2 cut(s)	1279, 1301
SfcI	CTRYAG	2 cut(s)	567, 639
SinI	GGWCC	2 cut(s)	176, 308
SmiMI	CAYNNNNRTG	3 cut(s)	119, 252, 1235
SmlI	CTYRAG	2 cut(s)	586, 980
SmoI	CTYRAG	2 cut(s)	586, 980
SphI	GCATGC	1 cut(s)	704
Sse9I	AATT	8 cut(s)	407, 430, 505, 729, 759, 804, 1020, 1173
SsiI	CCGC	3 cut(s)	76, 117, 338
SspMI	CTAG	3 cut(s)	267, 533, 782
StyD4I	CCNGG	5 cut(s)	178, 259, 816, 948, 1015
StyI	CCWWGG	3 cut(s)	156, 289, 323
TaaI	ACNGT	2 cut(s)	112, 1261
TaiI	ACGT	3 cut(s)	612, 870, 1316
TaqI	TCGA	4 cut(s)	285, 737, 755, 858
TaqII	GACCGA	1 cut(s)	291
TasI	AATT	8 cut(s)	407, 430, 505, 729, 759, 804, 1020, 1173
TatI	WGTACW	2 cut(s)	136, 375
TfiI	GAWTC	1 cut(s)	238
Tru1I	TTAA	3 cut(s)	587, 882, 1176
Tru9I	TTAA	3 cut(s)	587, 882, 1176
TscAI	CASTG	1 cut(s)	711
TseFI	GTSAC	5 cut(s)	112, 365, 382, 549, 1078
TseI	GCWGC	5 cut(s)	536, 539, 905, 908, 942
Tsp45I	GTSAC	5 cut(s)	112, 365, 382, 549, 1078
TspDTI	ATGAA	6 cut(s)	264, 470, 1019, 1131, 1219, 1231
TspRI	CASTG	1 cut(s)	711
Vha464I	CTTAAG	1 cut(s)	586
VpaK11BI	GGWCC	2 cut(s)	176, 308
XapI	RAATTY	2 cut(s)	407, 759
XceI	RCATGY	4 cut(s)	426, 652, 704, 979
XmnI	GAANNNNTTC	1 cut(s)	255
XspI	CTAG	3 cut(s)	267, 533, 782
Zsp2I	ATGCAT	3 cut(s)	622, 670, 1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.