RLG00000006569

Lysine histidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011639
Physical Location & Seq
Forward (+)
8581012 .. 8583356
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006569

Sequence Viewer

Length: 1317 bp
ATGGTTACAACTTCTCCTCCTCCAAAGCAGGAAGCTCTTGCAGACAACAAATGGAAGGAGGAGGCAGACACCACGCGCCGAGCCAAATGGTGGTACTCCACCTTCCACACCGTCACCGCCATGATAGGCGCCGGTGTCCTCAGCCTACCATATGCCATGGCCTACTTAGGATGGGGTCCAGGGACAATGGTAATGGCAGTCTCTTGGTGCATGACCTTGAACACAATGTGGCAGATGATACAACTCCATGAATGTGTTCCAGGGGTTCGCTTCGACCGATACATAGACCTTGGTCGACACGCCTTCGGTCCTAAACTCGGGCCATGGATTGTGCTTCCGCAGCAGTTAATTGTCCAAGTTGGGTGTGACATAGTGTACATGGTCACTGGGGGAAAGTGTCTGAAGCAATTTTTGGAGATGGCATGTTCCAGTTGCACACCAGTCAAGCAATCCTACTGGATTTTGATCTTTGGTTCCATTCATTTCGTCCTCTCCCAGCTTCCCAATTTCAACTCTGTCGCTGGTGTTTCACTAGCAGCAGCTATCATGTCACTAAGTTATTCAACAATAGCTTGGGCTGGTTGCTTAAGTAAAGGCCAGGTCGACAATGTTAGCTATGCATACAAGAAAACTAGCCCAGCTGATTACATGTTCCGGGTGTTCAATTCCCTTGGTCAAATCTCGTTTGCATTTGCTGGACATGCTGTGGCTCTTGAAATTCAGGCCACAATTCCTTCGACTCCAGAGAGGCCTTCAAGAATACCGATGTGGAAAGGTGCCGTCGGAGCCTATTTTATCAATGCAATTTGCTATTTCCCGGTGGCCCTCATCGGTTACTGGGCTTTTGGGCAAGATGTTGATGATAATGTGCTAGTGGATCTCAAGAGACCTGCTTGGCTCATTGCATCTGCAAATTTAATGGTTGTTGTCCATGTTATTGGCAGCTATCAGGTTTATGCTATGCCGGTTTTTGACCTGCTAGAGAGGATGATGATGAACAAACTGAACTTCCCACCTGGAGTGGCACTTAGACTAGTTGCTAGATCAGCTTATGTTGCATTCACATTGTTTGTCGGAGTCACCTTCCCTTTCTTTGGAGACCTTCTTGGTTTCTTCGGCGGATTTGGTTTTGCCCCGACTTCATACTTTCTTCCTAGTATAATGTGGCTGATTATCAAGAAACCAAAGAGATTCAGCACCAACTGGTTCATCAACTGGGTTTCCATTTTCATTGGAGTGTTCATTATGTTGGCCTCCACAGTTGGTGGTTTGAGGAACATCATCACTGATGCATCCACATATCGTTTCTACACTTAA

Protein Analysis

439

Amino Acids

48.83

Weight (kDa)

9.14

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 27 - 422 7.4e-99 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 29 - 160 1.3e-06 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011639)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25530
fragaria_vesca FvH4_4g27830 FvH4_4g27960 FvH4_4g27960 FvH4_4g27960 FvH4_4g27960
malus_domestica MD13G1045300.v1.1 MD16G1046600.v1.1 MD16G1047100.v1.1
prunus_persica Prupe.1G306700_v2.0.a1 Prupe.1G306700_v2.0.a1
pyrus_communis pycom13g03990 pycom16g04100
rosa_chinensis RchiOBHm_Chr4g0435661
rosa_laevigata RLG00000006569
rosa_multiflora Rmu_sc0000051.1_g000010
rosa_rugosa Rorug04G0287800
rosa_samantha Rh4AG342900
rosa_wichuraiana Rw4G029930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 898, 984
AccB1I GGYRCC 2 cut(s) 128, 776
AccB7I CCANNNNNTGG 1 cut(s) 90
AccI GTMKAC 2 cut(s) 295, 603
AccII CGCG 1 cut(s) 76
AciI CCGC 3 cut(s) 117, 338, 1119
AclWI GGATC 1 cut(s) 885
AcsI RAATTY 2 cut(s) 717, 913
AcuI CTGAAG 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 129
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 2 cut(s) 95, 377
AfiI CCNNNNNNNGG 3 cut(s) 90, 317, 1094
AflII CTTAAG 1 cut(s) 586
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 6 cut(s) 220, 511, 564, 664, 716, 756
AhlI ACTAGT 1 cut(s) 1033
AjnI CCWGG 4 cut(s) 178, 259, 597, 1015
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AluBI AGCT 8 cut(s) 35, 499, 542, 572, 615, 641, 945, 1049
AluI AGCT 8 cut(s) 35, 499, 542, 572, 615, 641, 945, 1049
Alw26I GTCTC 3 cut(s) 205, 880, 1092
AlwI GGATC 1 cut(s) 885
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 7 cut(s) 159, 320, 595, 723, 749, 822, 1251
ApeKI GCWGC 4 cut(s) 340, 536, 539, 942
ApoI RAATTY 2 cut(s) 717, 913
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 2 cut(s) 78, 131
AspS9I GGNCC 4 cut(s) 176, 308, 320, 823
AsuC2I CCSGG 2 cut(s) 656, 818
AsuHPI GGTGA 2 cut(s) 106, 1072
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 2 cut(s) 176, 308
BanI GGYRCC 2 cut(s) 128, 776
BarI GAAGNNNNNNTAC 2 cut(s) 86, 118
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 4 cut(s) 352, 548, 551, 954
BccI CCATC 2 cut(s) 165, 412
BceAI ACGGC 1 cut(s) 764
BciT130I CCWGG 4 cut(s) 180, 261, 599, 1017
BcnI CCSGG 2 cut(s) 656, 818
BcoDI GTCTC 3 cut(s) 205, 880, 1092
BcuI ACTAGT 1 cut(s) 1033
BfaI CTAG 7 cut(s) 533, 633, 872, 980, 1034, 1041, 1155
BfoI RGCGCY 1 cut(s) 132
BfrI CTTAAG 1 cut(s) 586
BfuAI ACCTGC 2 cut(s) 898, 984
BisI GCNGC 4 cut(s) 341, 537, 540, 943
BlsI GCNGC 4 cut(s) 342, 538, 541, 944
Bme1390I CCNGG 6 cut(s) 180, 261, 599, 656, 818, 1017
Bme18I GGWCC 2 cut(s) 176, 308
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 4 cut(s) 176, 308, 320, 823
BmiI GGNNCC 5 cut(s) 130, 177, 475, 778, 787
BmrFI CCNGG 6 cut(s) 180, 261, 599, 656, 818, 1017
BmrI ACTGGG 3 cut(s) 396, 847, 1225
BmsI GCATC 3 cut(s) 914, 1279, 1301
BmuI ACTGGG 3 cut(s) 396, 847, 1225
BoxI GACNNNNGTC 1 cut(s) 291
BpmI CTGGAG 2 cut(s) 726, 1038
Bpu10I CCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 866
BpuMI CCSGG 2 cut(s) 656, 818
BsaBI GATNNNNATC 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 2 cut(s) 880, 1092
BsaJI CCNNGG 6 cut(s) 156, 179, 260, 289, 323, 670
BsaXI ACNNNNNCTCC 2 cut(s) 28, 31
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 317, 1094
Bse118I RCCGGY 2 cut(s) 131, 964
Bse1I ACTGG 7 cut(s) 391, 429, 440, 461, 842, 1208, 1220
Bse3DI GCAATG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 464
BseBI CCWGG 4 cut(s) 180, 261, 599, 1017
BseDI CCNNGG 6 cut(s) 156, 179, 260, 289, 323, 670
BseGI GGATG 3 cut(s) 176, 993, 1292
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 3 cut(s) 90, 317, 1094
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 7 cut(s) 391, 429, 440, 461, 842, 1208, 1220
BseRI GAGGAG 3 cut(s) 6, 9, 74
BseXI GCAGC 4 cut(s) 352, 548, 551, 954
BseYI CCCAGC 2 cut(s) 495, 637
Bsh1236I CGCG 1 cut(s) 76
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 7 cut(s) 161, 322, 597, 725, 751, 824, 1253
BshNI GGYRCC 2 cut(s) 128, 776
BsiEI CGRYCG 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 4 cut(s) 132, 655, 818, 965
BslFI GGGAC 1 cut(s) 196
BslI CCNNNNNNNGG 3 cut(s) 90, 317, 1094
BsmAI GTCTC 3 cut(s) 205, 880, 1092
BsmFI GGGAC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 1058
BsnI GGCC 7 cut(s) 161, 322, 597, 725, 751, 824, 1253
Bso31I GGTCTC 2 cut(s) 880, 1092
BsoBI CYCGRG 1 cut(s) 317
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 3 cut(s) 465, 877, 1043
Bsp19I CCATGG 2 cut(s) 156, 323
BspACI CCGC 3 cut(s) 117, 338, 1119
BspANI GGCC 7 cut(s) 161, 322, 597, 725, 751, 824, 1253
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 5 cut(s) 130, 177, 475, 778, 787
BspMI ACCTGC 2 cut(s) 898, 984
BspPI GGATC 1 cut(s) 885
BspT107I GGYRCC 2 cut(s) 128, 776
BspTI CTTAAG 1 cut(s) 586
BspTNI GGTCTC 2 cut(s) 880, 1092
BsrDI GCAATG 1 cut(s) 900
BsrFI RCCGGY 2 cut(s) 131, 964
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 7 cut(s) 391, 429, 440, 461, 842, 1208, 1220
BssAI RCCGGY 2 cut(s) 131, 964
BssECI CCNNGG 6 cut(s) 156, 179, 260, 289, 323, 670
BssMI GATC 3 cut(s) 465, 877, 1043
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 4 cut(s) 156, 289, 323, 670
Bst2UI CCWGG 4 cut(s) 180, 261, 599, 1017
Bst4CI ACNGT 2 cut(s) 112, 1261
BstACI GRCGYC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 586
BstAUI TGTACA 1 cut(s) 375
BstDEI CTNAG 4 cut(s) 140, 166, 554, 1028
BstDSI CCRYGG 2 cut(s) 156, 323
BstF5I GGATG 3 cut(s) 176, 993, 1292
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 2 cut(s) 78, 131
BstKTI GATC 3 cut(s) 468, 880, 1046
BstMAI GTCTC 3 cut(s) 205, 880, 1092
BstMBI GATC 3 cut(s) 465, 877, 1043
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 5 cut(s) 340, 701, 785, 1046, 1055
BstNI CCWGG 4 cut(s) 180, 261, 599, 1017
BstNSI RCATGY 3 cut(s) 426, 652, 704
BstPAI GACNNNNGTC 1 cut(s) 291
BstSCI CCNGG 6 cut(s) 178, 259, 597, 654, 816, 1015
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 4 cut(s) 352, 548, 551, 954
BstX2I RGATCY 1 cut(s) 877
BstXI CCANNNNNNTGG 1 cut(s) 938
BstYI RGATCY 1 cut(s) 877
BsuRI GGCC 7 cut(s) 161, 322, 597, 725, 751, 824, 1253
BtgI CCRYGG 2 cut(s) 156, 323
BtsCI GGATG 3 cut(s) 176, 993, 1292
BtsIMutI CAGTG 2 cut(s) 384, 1284
BveI ACCTGC 2 cut(s) 898, 984
CfoI GCGC 2 cut(s) 78, 131
Cfr10I RCCGGY 2 cut(s) 131, 964
Cfr13I GGNCC 4 cut(s) 176, 308, 320, 823
Csp6I GTAC 2 cut(s) 94, 376
CviQI GTAC 2 cut(s) 94, 376
DdeI CTNAG 4 cut(s) 140, 166, 554, 1028
DinI GGCGCC 1 cut(s) 130
DpnI GATC 3 cut(s) 467, 879, 1045
DpnII GATC 3 cut(s) 465, 877, 1043
DraIII CACNNNGTG 1 cut(s) 228
EciI GGCGGA 1 cut(s) 1134
Eco130I CCWWGG 4 cut(s) 156, 289, 323, 670
Eco147I AGGCCT 1 cut(s) 751
Eco31I GGTCTC 2 cut(s) 880, 1092
Eco47I GGWCC 2 cut(s) 176, 308
Eco57I CTGAAG 1 cut(s) 422
Eco88I CYCGRG 1 cut(s) 317
EcoRII CCWGG 4 cut(s) 178, 259, 597, 1015
EcoT14I CCWWGG 4 cut(s) 156, 289, 323, 670
EcoT22I ATGCAT 2 cut(s) 622, 1294
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 4 cut(s) 156, 289, 323, 670
FaqI GGGAC 1 cut(s) 196
FauNDI CATATG 1 cut(s) 151
FblI GTMKAC 2 cut(s) 295, 603
Fnu4HI GCNGC 4 cut(s) 341, 537, 540, 943
FokI GGATG 3 cut(s) 183, 1000, 1279
Fsp4HI GCNGC 4 cut(s) 341, 537, 540, 943
FspBI CTAG 7 cut(s) 533, 633, 872, 980, 1034, 1041, 1155
GlaI GCGC 2 cut(s) 77, 130
GluI GCNGC 4 cut(s) 341, 537, 540, 943
GsaI CCCAGC 2 cut(s) 499, 641
GsuI CTGGAG 2 cut(s) 726, 1038
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 7 cut(s) 161, 322, 597, 725, 751, 824, 1253
HapII CCGG 4 cut(s) 132, 655, 818, 965
HhaI GCGC 2 cut(s) 78, 131
Hin1I GRCGYC 1 cut(s) 129
Hin6I GCGC 2 cut(s) 76, 129
HinP1I GCGC 2 cut(s) 76, 129
HincII GTYRAC 2 cut(s) 296, 604
HindII GTYRAC 2 cut(s) 296, 604
HinfI GANTC 3 cut(s) 739, 1077, 1191
HpaII CCGG 4 cut(s) 132, 655, 818, 965
HphI GGTGA 2 cut(s) 106, 1072
Hpy166II GTNNAC 3 cut(s) 296, 376, 604
Hpy188I TCNGA 3 cut(s) 402, 785, 1076
Hpy188III TCNNGA 5 cut(s) 713, 743, 756, 883, 1177
Hpy8I GTNNAC 3 cut(s) 296, 376, 604
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 7 cut(s) 49, 112, 313, 744, 762, 1093, 1112
HpyCH4III ACNGT 2 cut(s) 112, 1261
HpyF10VI GCNNNNNNNGC 5 cut(s) 340, 701, 785, 1046, 1055
HpyF3I CTNAG 4 cut(s) 140, 166, 554, 1028
Hsp92I GRCGYC 1 cut(s) 129
HspAI GCGC 2 cut(s) 76, 129
KasI GGCGCC 1 cut(s) 128
Kzo9I GATC 3 cut(s) 465, 877, 1043
LmnI GCTCC 1 cut(s) 785
Lsp1109I GCAGC 4 cut(s) 352, 548, 551, 954
LweI GCATC 3 cut(s) 914, 1279, 1301
MaeI CTAG 7 cut(s) 533, 633, 872, 980, 1034, 1041, 1155
MaeIII GTNAC 7 cut(s) 4, 112, 365, 382, 549, 833, 1078
MalI GATC 3 cut(s) 467, 879, 1045
MboI GATC 3 cut(s) 465, 877, 1043
MboII GAAGA 2 cut(s) 1105, 1142
MflI RGATCY 1 cut(s) 877
MluCI AATT 8 cut(s) 348, 407, 505, 664, 717, 729, 804, 913
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 2 cut(s) 733, 1086
MmeI TCCRAC 2 cut(s) 763, 1054
Mph1103I ATGCAT 2 cut(s) 622, 1294
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 4 cut(s) 347, 587, 917, 1315
MslI CAYNNNNRTG 3 cut(s) 119, 252, 1235
MspA1I CMGCKG 1 cut(s) 641
MspCI CTTAAG 1 cut(s) 586
MspI CCGG 4 cut(s) 132, 655, 818, 965
MspR9I CCNGG 6 cut(s) 180, 261, 599, 656, 818, 1017
Mva1269I GAATGC 1 cut(s) 1058
MvaI CCWGG 4 cut(s) 180, 261, 599, 1017
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 5 cut(s) 340, 701, 785, 1046, 1055
NarI GGCGCC 1 cut(s) 129
NciI CCSGG 2 cut(s) 656, 818
NcoI CCATGG 2 cut(s) 156, 323
NdeI CATATG 1 cut(s) 151
NdeII GATC 3 cut(s) 465, 877, 1043
NlaIV GGNNCC 5 cut(s) 130, 177, 475, 778, 787
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 5 cut(s) 112, 365, 382, 549, 1078
NsiI ATGCAT 2 cut(s) 622, 1294
NspI RCATGY 3 cut(s) 426, 652, 704
PceI AGGCCT 1 cut(s) 751
PciI ACATGT 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 274
PctI GAATGC 1 cut(s) 1058
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 1191
PflMI CCANNNNNTGG 1 cut(s) 90
PkrI GCNGC 4 cut(s) 342, 538, 541, 944
PleI GAGTC 2 cut(s) 733, 1085
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 2 cut(s) 733, 1085
PscI ACATGT 1 cut(s) 648
PshAI GACNNNNGTC 1 cut(s) 291
Psp6I CCWGG 4 cut(s) 178, 259, 597, 1015
PspFI CCCAGC 2 cut(s) 495, 637
PspGI CCWGG 4 cut(s) 178, 259, 597, 1015
PspN4I GGNNCC 5 cut(s) 130, 177, 475, 778, 787
PspPI GGNCC 4 cut(s) 176, 308, 320, 823
PsuI RGATCY 1 cut(s) 877
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 2 cut(s) 95, 377
RsaNI GTAC 2 cut(s) 94, 376
RseI CAYNNNNRTG 3 cut(s) 119, 252, 1235
SalI GTCGAC 2 cut(s) 294, 602
SaqAI TTAA 4 cut(s) 347, 587, 917, 1315
SatI GCNGC 4 cut(s) 341, 537, 540, 943
Sau3AI GATC 3 cut(s) 465, 877, 1043
Sau96I GGNCC 4 cut(s) 176, 308, 320, 823
SchI GAGTC 2 cut(s) 733, 1086
ScrFI CCNGG 6 cut(s) 180, 261, 599, 656, 818, 1017
SfaNI GCATC 3 cut(s) 914, 1279, 1301
SfoI GGCGCC 1 cut(s) 130
SgrAI CRCCGGYG 1 cut(s) 131
SinI GGWCC 2 cut(s) 176, 308
SmiMI CAYNNNNRTG 3 cut(s) 119, 252, 1235
SmlI CTYRAG 2 cut(s) 586, 881
SmoI CTYRAG 2 cut(s) 586, 881
SpeI ACTAGT 1 cut(s) 1033
Sse9I AATT 8 cut(s) 348, 407, 505, 664, 717, 729, 804, 913
SseBI AGGCCT 1 cut(s) 751
SsiI CCGC 3 cut(s) 117, 338, 1119
SspDI GGCGCC 1 cut(s) 128
SspMI CTAG 7 cut(s) 533, 633, 872, 980, 1034, 1041, 1155
StuI AGGCCT 1 cut(s) 751
StyD4I CCNGG 6 cut(s) 178, 259, 597, 654, 816, 1015
StyI CCWWGG 4 cut(s) 156, 289, 323, 670
TaaI ACNGT 2 cut(s) 112, 1261
TaqI TCGA 4 cut(s) 273, 295, 603, 737
TaqII GACCGA 2 cut(s) 291, 296
TasI AATT 8 cut(s) 348, 407, 505, 664, 717, 729, 804, 913
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 1 cut(s) 1191
Tru1I TTAA 4 cut(s) 347, 587, 917, 1315
Tru9I TTAA 4 cut(s) 347, 587, 917, 1315
TscAI CASTG 2 cut(s) 391, 1291
TseFI GTSAC 5 cut(s) 112, 365, 382, 549, 1078
TseI GCWGC 4 cut(s) 340, 536, 539, 942
Tsp45I GTSAC 5 cut(s) 112, 365, 382, 549, 1078
TspDTI ATGAA 7 cut(s) 264, 470, 1010, 1131, 1198, 1219, 1231
TspRI CASTG 2 cut(s) 391, 1291
Van91I CCANNNNNTGG 1 cut(s) 90
Vha464I CTTAAG 1 cut(s) 586
VpaK11BI GGWCC 2 cut(s) 176, 308
XapI RAATTY 2 cut(s) 717, 913
XceI RCATGY 3 cut(s) 426, 652, 704
XmiI GTMKAC 2 cut(s) 295, 603
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 7 cut(s) 533, 633, 872, 980, 1034, 1041, 1155
Zsp2I ATGCAT 2 cut(s) 622, 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.