Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006693

Physical Location & Seq

Genomic Coordinates Reverse (-)

3865539 .. 3869352

Gene Structure 3814 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1054700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGCTCCATCACATCCACAAATGGAAGCCCTTACATTTTCTACAAAAATTTCAGATTCTCGCTCCCCTCTCCTCCCTCATCTTCACTAAACCTTCGGCGTCCGCTGCAAAGCTCGAAGACGAGCTCGCCGCCGCCGCCGCCATCCCAAATCCTAGAAACACCGTCTCCGAAGTCATTACCGGCTTGGGAATCTTTGGTTTGAGAAAATTCTTGGGTAATTGTTATTTTAGAACCATGGTCTCAAAGTTGAATCAGCCGGAGGTGGATTTGATTATTGAAAGTTTGAGTCTTGAGAGCTCGGATTCAGCCTTTGGGTTCTTCAAGTTTTTGAGGAATGAGTGTGGCTTTCGGCATTCGAGGATTTCGGAGTTTATTGTTGTTCATGTATTGGCGACGAACTGGCAGTTTCAGGAGTTGCGTTCGGTTGTAAAACAAATGGTGGATGAGGAGGGCCCTGGTTCTGCACCTTCACTTTGTGAGCTGCTCTTGTATAGATTCAGGGACTGGGACTCAAGTAGTGTGGTGTGGGATATGTTGGCGTTTGCTTATTCGAGATCTGAAATGGTTCATGATGCCCTGTCTGTTCTTGCTAAGATGAAAGATTTGAATTTGAAGGTTTCGACATCAACCTACAACTGCCTGTTGCATAATTTGAGGCATACAGATATCATGTGGAATGTTTATAATGAAATAAAAGATAGTGGAACTCCAGAGAGTGACTACACTACGTCTATACTTATAGATGGATTATGCCAGCAATCCAGCGTGCAAGATGCAGTTTCCTTCCTCATGGATGCTGAAAGGACGGAGACTGGGCCTTCAGTTGTTTCATTCAATACCATCATGTCTAGGTTCTGTAAATTGGGTTTTGTGGATGTTGCGAAGTCGTTTTTTTGTGTGATGTTCAAGTATGGATTAGTTCCTGATTCGTACAGTTATAATATTCTGATTCATGGGCTATGTGTAGCAGGTTCATTGGAAGAAGCATTGGAGTTCACTAAAGACATGGAGAGGCATGGGGTGCAGCCTGACACGGTAACTTACAACATTCTCTGTAAGGGGTTTCATCTACTTGGTTTGATGAGTGGAGCTCGCAAGGTCATTCAGAAGATGTTGGTTAAAGGGTTGAATCCGGATCATGTTACATATACAATTATGATATGTGGCCACTGCCATGTTGGCAATATTGACGAAGCTCTTAAGTTGCAAAAAGAGATGATTTCAAGAGGTTTTCAGTTGAGTGTCATTGTATACAGTGTACTACTCAGCAGTATGTGTAAAAGTGGACGAGTAGAAGAAGCATTGAGATTGCTGTATGAGATGGAAGCTGTTGGCTTGGAACCAGATCTTATAACATATTCTATCCTCATTCATGGTCTATGCAAGCAAGGAGACGTACAAAGGGCTAGTGAAATATATAGAGAAATGTATATGAAGAGAATCATTCCCAATTACTTTGCACACCGTGCTATTCTGTTAGGTCTGCGTGAGAAAGGGGATATATATGAGGCAAGGAAGTATTTTGATCATCTGACGACAAGGGCTGTGACAGAGGATATTGTGCTGTATAATATTATGATGGATGGGTACGTAAAACTAGGTAATGTTGCAGAAGCGATACAATTATATAAACAAATAATTGAGAAGGGATTAAATCCCAGTACTGTCACTTTCAATACTCTTATCCATGGTTTCTGCAAAAATGGGAAACTAGTGGAGGCTCGAAGGATGTTGGATACCATCGAGCTGCATGGATTGCTACCAAGTCCAGTTACTTACACTACTCTTATGAATGCTAACTGTGAACAGGGAAACATCAATGGCATGCTTGAATTGCTTCGAGAGATGGAAGCGAAAGATGTAGAACCAACTCATGTATCTTACACGGTAGTTATTAAAGGACTTTGCAGACAGGGGAAGCGGTGGGATGCTGTTCACTTAGTTGAGGAAATGTATGCCAAGGGCCTAAGCCCGGATCAAATCACATATAATACAATCATCAAATGTTTCTGCAAAGCTCAAGACTTCGAGAAAGCTTTTCAGTTACACAACGAGATGTTAATGCATAATCTTGCGCCTACTCCTGTTACGTATAATCTCCTCATCAATGGTCTTTGTGTATATGGTGACCTAGAGGATGCTGACAGGCTGCTGGTTTCTCTCAATGACAGCAATATCAATTTGACAAAAGTTGCCTATACCACGTTAATAAAAGCACATTGTGCAAAGGGTGATGTATATAGGGCCGTGGCTCTCTTTCATCAAATGGTGGAGAAAGGATTTGAAATTTCCATCAGAGATTATAGTGCTGTAATTAATAGATTGTGCAAAAGGTGTTGGATAACTGAAGCAAAATACTTTTTCTGTATGATGTTATCTGATGGGATTTCTCCTGATCAAGAACTCTGTGAGGTGATGCTTAATGTTTTTTATCAAGGTGGTGATTTCGATTCAGCAGCGGAACTCCTTGCTGAGATGATCAAATTTGGCTTTCGTCCGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

835

Amino Acids

94.47

Weight (kDa)

6.03

Isoelectric Point (pI)

37.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	273 - 321	3e-14	PPR repeat family
PPR_3	PF13812	304 - 353	1.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	305 - 337	9.3e-10	PPR repeat
PPR	PF01535	311 - 341	4.7e-07	PPR repeat
PPR_2	PF13041	312 - 355	7.2e-14	PPR repeat family
PPR_2	PF13041	359 - 392	9.9e-07	PPR repeat family
PPR_1	PF12854	374 - 406	8.3e-11	PPR repeat
PPR_2	PF13041	378 - 427	6.3e-13	PPR repeat family
PPR	PF01535	381 - 411	4.2e-08	PPR repeat
PPR_1	PF12854	409 - 442	1.3e-07	PPR repeat
PPR_2	PF13041	414 - 462	2.9e-15	PPR repeat family
PPR	PF01535	417 - 445	5.7e-06	PPR repeat
PPR_3	PF13812	436 - 492	2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	445 - 476	4.9e-12	PPR repeat
PPR	PF01535	451 - 478	5.7e-06	PPR repeat
TPR_24	PF23276	452 - 546	1e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	519 - 567	9.3e-16	PPR repeat family
PPR	PF01535	521 - 551	6e-07	PPR repeat
PPR_long	PF17177	535 - 639	4.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	549 - 580	1.7e-12	PPR repeat
PPR_2	PF13041	553 - 601	2.6e-13	PPR repeat family
PPR	PF01535	556 - 585	3.3e-06	PPR repeat
PPR_3	PF13812	577 - 633	3.6e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	588 - 637	2e-13	PPR repeat family
PPR_1	PF12854	619 - 651	1.2e-07	PPR repeat
PPR_2	PF13041	623 - 672	5e-14	PPR repeat family
PPR_long	PF17177	645 - 745	4.6e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	647 - 703	1.3e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	654 - 686	7.4e-12	PPR repeat
TPR_24	PF23276	654 - 755	8e-09	Fungal tetratrico peptide repeats
PPR_2	PF13041	658 - 706	4.8e-17	PPR repeat family
PPR	PF01535	661 - 690	7.5e-07	PPR repeat
PPR_2	PF13041	693 - 741	4.1e-09	PPR repeat family
PPR_1	PF12854	726 - 757	3.7e-06	PPR repeat
PPR	PF01535	731 - 761	1.6e-06	PPR repeat
PPR_2	PF13041	731 - 777	9.2e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006693)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13630 AT1G13630 AT1G13630 AT1G13630 AT1G13630 AT1G13630
fragaria_vesca	FvH4_4g29120
malus_domestica	MD13G1055600.v1.1 MD16G1054700.v1.1
prunus_persica	Prupe.2G062100_v2.0.a1 Prupe.2G062200_v2.0.a1 Prupe.2G062300_v2.0.a1 Prupe.7G020200_v2.0.a1 Prupe.7G020300_v2.0.a1
pyrus_communis	pycom16g04760
rosa_chinensis	RchiOBHm_Chr4g0437181
rosa_laevigata	RLG00000006457
rosa_multiflora	Rmu_co8390257.1_g000001 Rmu_sc0000591.1_g000006
rosa_roxburghii	Rroxscaffold_5G00378320
rosa_rugosa	Rorug04G0298500.1
rosa_samantha	Rh4AG353500 Rh4BG363300 Rh4CG377500 Rh4DG357300
rosa_wichuraiana	Rw4G030970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	684, 939, 1352
Acc36I	ACCTGC	1 cut(s)	959
AccI	GTMKAC	1 cut(s)	1251
AccIII	TCCGGA	2 cut(s)	1132, 2497
AciI	CCGC	7 cut(s)	102, 129, 132, 135, 138, 1921, 2459
AclWI	GGATC	2 cut(s)	1143, 1983
AcoI	YGGCCR	1 cut(s)	1165
AcsI	RAATTY	5 cut(s)	47, 206, 607, 2286, 2483
AcuI	CTGAAG	2 cut(s)	804, 2367
AcyI	GRCGYC	1 cut(s)	98
AdeI	CACNNNGTG	3 cut(s)	476, 1466, 2222
AfaI	GTAC	5 cut(s)	932, 1260, 1398, 1589, 1663
AfiI	CCNNNNNNNGG	1 cut(s)	1972
AflII	CTTAAG	1 cut(s)	1199
AhlI	ACTAGT	1 cut(s)	1711
AjnI	CCWGG	1 cut(s)	454
Alw21I	GWGCWC	3 cut(s)	126, 299, 1093
Alw26I	GTCTC	4 cut(s)	169, 244, 803, 1386
AlwI	GGATC	2 cut(s)	1143, 1983
AlwNI	CAGNNNCTG	1 cut(s)	504
Aor13HI	TCCGGA	2 cut(s)	1132, 2497
AoxI	GGCC	5 cut(s)	451, 815, 1165, 1963, 2244
ApaI	GGGCCC	1 cut(s)	455
ApeKI	GCWGC	6 cut(s)	104, 481, 1024, 1747, 2149, 2456
ApoI	RAATTY	5 cut(s)	47, 206, 607, 2286, 2483
AseI	ATTAAT	1 cut(s)	2316
Asp700I	GAANNNNTTC	2 cut(s)	564, 1836
AspLEI	GCGC	1 cut(s)	2077
AspS9I	GGNCC	5 cut(s)	451, 452, 815, 1963, 2244
AsuC2I	CCSGG	1 cut(s)	1973
AsuHPI	GGTGA	4 cut(s)	2138, 2243, 2425, 2453
BaeGI	GKGCMC	1 cut(s)	455
BaeI	ACNNNNGTAYC	2 cut(s)	1860, 1893
BalI	TGGCCA	1 cut(s)	1167
BanII	GRGCYC	4 cut(s)	126, 299, 455, 1093
BbsI	GAAGAC	1 cut(s)	123
Bbv12I	GWGCWC	3 cut(s)	126, 299, 1093
BbvI	GCAGC	6 cut(s)	91, 468, 1036, 1734, 2136, 2468
BceAI	ACGGC	1 cut(s)	2231
BciT130I	CCWGG	1 cut(s)	456
BciVI	GTATCC	1 cut(s)	1729
BclI	TGATCA	3 cut(s)	1525, 2395, 2478
BcnI	CCSGG	1 cut(s)	1973
BcoDI	GTCTC	4 cut(s)	169, 244, 803, 1386
BcuI	ACTAGT	1 cut(s)	1711
BfaI	CTAG	6 cut(s)	153, 849, 1407, 1598, 1712, 2132
BfrI	CTTAAG	1 cut(s)	1199
BfuAI	ACCTGC	1 cut(s)	959
BfuI	GTATCC	1 cut(s)	1729
BglI	GCCNNNNNGGC	1 cut(s)	1179
BglII	AGATCT	2 cut(s)	554, 1345
BmcAI	AGTACT	1 cut(s)	1663
Bme1390I	CCNGG	2 cut(s)	456, 1973
BmgT120I	GGNCC	5 cut(s)	451, 452, 815, 1963, 2244
BmiI	GGNNCC	2 cut(s)	453, 1341
BmrFI	CCNGG	2 cut(s)	456, 1973
BmrI	ACTGGG	3 cut(s)	514, 822, 1653
BmsI	GCATC	6 cut(s)	562, 763, 784, 1918, 2128, 2406
BmuI	ACTGGG	3 cut(s)	514, 822, 1653
BpiI	GAAGAC	1 cut(s)	123
BplI	GAGNNNNNCTC	2 cut(s)	1075, 1107
BpmI	CTGGAG	1 cut(s)	693
Bpu10I	CCTNAGC	1 cut(s)	1967
BpuEI	CTTGAG	3 cut(s)	311, 496, 2004
BpuMI	CCSGG	1 cut(s)	1973
BsaAI	YACGTR	2 cut(s)	1591, 2091
BsaHI	GRCGYC	1 cut(s)	98
BsaI	GGTCTC	1 cut(s)	244
BsaJI	CCNNGG	5 cut(s)	234, 454, 1687, 1959, 2247
BsaWI	WCCGGW	2 cut(s)	1132, 2497
BsaXI	ACNNNNNCTCC	2 cut(s)	149, 179
Bsc4I	CCNNNNNNNGG	1 cut(s)	1972
Bse118I	RCCGGY	1 cut(s)	179
Bse1I	ACTGG	5 cut(s)	404, 509, 817, 1659, 1769
BseAI	TCCGGA	2 cut(s)	1132, 2497
BseBI	CCWGG	1 cut(s)	456
BseDI	CCNNGG	5 cut(s)	234, 454, 1687, 1959, 2247
BseGI	GGATG	9 cut(s)	12, 141, 448, 799, 880, 1588, 1734, 1933, 2143
BseLI	CCNNNNNNNGG	1 cut(s)	1972
BseMII	CTCAG	2 cut(s)	1279, 2463
BseNI	ACTGG	5 cut(s)	404, 509, 817, 1659, 1769
BseRI	GAGGAG	3 cut(s)	61, 461, 2090
BseSI	GKGCMC	1 cut(s)	455
BseXI	GCAGC	6 cut(s)	91, 468, 1036, 1734, 2136, 2468
BsgI	GTGCAG	2 cut(s)	447, 1043
BshFI	GGCC	5 cut(s)	453, 817, 1167, 1965, 2246
BsiHKAI	GWGCWC	3 cut(s)	126, 299, 1093
BsiSI	CCGG	5 cut(s)	180, 257, 1133, 1973, 2498
BslFI	GGGAC	2 cut(s)	515, 521
BslI	CCNNNNNNNGG	1 cut(s)	1972
BsmAI	GTCTC	4 cut(s)	169, 244, 803, 1386
BsmBI	CGTCTC	2 cut(s)	169, 1386
BsmFI	GGGAC	2 cut(s)	515, 521
BsmI	GAATGC	2 cut(s)	352, 1798
BsnI	GGCC	5 cut(s)	453, 817, 1167, 1965, 2246
Bso31I	GGTCTC	1 cut(s)	244
Bsp120I	GGGCCC	1 cut(s)	451
Bsp1286I	GDGCHC	4 cut(s)	126, 299, 455, 1093
Bsp13I	TCCGGA	2 cut(s)	1132, 2497
Bsp143I	GATC	7 cut(s)	554, 1135, 1345, 1525, 1975, 2395, 2478
Bsp19I	CCATGG	2 cut(s)	234, 1687
BspACI	CCGC	7 cut(s)	102, 129, 132, 135, 138, 1921, 2459
BspANI	GGCC	5 cut(s)	453, 817, 1167, 1965, 2246
BspCNI	CTCAG	2 cut(s)	1278, 2464
BspEI	TCCGGA	2 cut(s)	1132, 2497
BspHI	TCATGA	1 cut(s)	568
BspLI	GGNNCC	2 cut(s)	453, 1341
BspMI	ACCTGC	1 cut(s)	959
BspPI	GGATC	2 cut(s)	1143, 1983
BspTI	CTTAAG	1 cut(s)	1199
BspTNI	GGTCTC	1 cut(s)	244
BsrFI	RCCGGY	1 cut(s)	179
BsrI	ACTGG	5 cut(s)	404, 509, 817, 1659, 1769
BssAI	RCCGGY	1 cut(s)	179
BssECI	CCNNGG	5 cut(s)	234, 454, 1687, 1959, 2247
BssMI	GATC	7 cut(s)	554, 1135, 1345, 1525, 1975, 2395, 2478
BssNAI	GTATAC	1 cut(s)	1252
BssNI	GRCGYC	1 cut(s)	98
BssT1I	CCWWGG	3 cut(s)	234, 1687, 1959
Bst1107I	GTATAC	1 cut(s)	1252
Bst2UI	CCWGG	1 cut(s)	456
Bst4CI	ACNGT	8 cut(s)	163, 935, 1036, 1256, 1466, 1666, 1802, 1888
Bst6I	CTCTTC	1 cut(s)	1430
BstACI	GRCGYC	1 cut(s)	98
BstAFI	CTTAAG	1 cut(s)	1199
BstAPI	GCANNNNNTGC	4 cut(s)	1021, 1466, 1756, 2222
BstBAI	YACGTR	2 cut(s)	1591, 2091
BstC8I	GCNNGC	6 cut(s)	126, 755, 767, 1093, 1385, 1826
BstDEI	CTNAG	5 cut(s)	591, 1265, 1939, 1967, 2472
BstDSI	CCRYGG	3 cut(s)	234, 1687, 2247
BstEII	GGTNACC	1 cut(s)	2126
BstF5I	GGATG	9 cut(s)	12, 141, 448, 799, 880, 1588, 1734, 1933, 2143
BstHHI	GCGC	1 cut(s)	2077
BstKTI	GATC	7 cut(s)	557, 1138, 1348, 1528, 1978, 2398, 2481
BstMAI	GTCTC	4 cut(s)	169, 244, 803, 1386
BstMBI	GATC	7 cut(s)	554, 1135, 1345, 1525, 1975, 2395, 2478
BstMWI	GCNNNNNNNGC	9 cut(s)	104, 134, 137, 1021, 1179, 1466, 1756, 1834, 2222
BstNI	CCWGG	1 cut(s)	456
BstNSI	RCATGY	1 cut(s)	1828
BstPI	GGTNACC	1 cut(s)	2126
BstSCI	CCNGG	2 cut(s)	454, 1971
BstSLI	GKGCMC	1 cut(s)	455
BstSNI	TACGTA	2 cut(s)	1591, 2091
BstV1I	GCAGC	6 cut(s)	91, 468, 1036, 1734, 2136, 2468
BstV2I	GAAGAC	1 cut(s)	123
BstX2I	RGATCY	2 cut(s)	554, 1345
BstYI	RGATCY	2 cut(s)	554, 1345
BstZ17I	GTATAC	1 cut(s)	1252
BsuI	GTATCC	1 cut(s)	1729
BsuRI	GGCC	5 cut(s)	453, 817, 1167, 1965, 2246
BtgI	CCRYGG	3 cut(s)	234, 1687, 2247
BtsCI	GGATG	9 cut(s)	12, 141, 448, 799, 880, 1588, 1734, 1933, 2143
BtsI	GCAGTG	1 cut(s)	1168
BtsIMutI	CAGTG	2 cut(s)	1168, 1261
BveI	ACCTGC	1 cut(s)	959
Cac8I	GCNNGC	6 cut(s)	126, 755, 767, 1093, 1385, 1826
CaiI	CAGNNNCTG	1 cut(s)	504
CciI	TCATGA	1 cut(s)	568
CfoI	GCGC	1 cut(s)	2077
Cfr10I	RCCGGY	1 cut(s)	179
Cfr13I	GGNCC	5 cut(s)	451, 452, 815, 1963, 2244
CseI	GACGC	1 cut(s)	87
Csp6I	GTAC	5 cut(s)	931, 1259, 1397, 1588, 1662
CspCI	CAANNNNNGTGG	2 cut(s)	501, 536
CviQI	GTAC	5 cut(s)	931, 1259, 1397, 1588, 1662
DdeI	CTNAG	5 cut(s)	591, 1265, 1939, 1967, 2472
DpnI	GATC	7 cut(s)	556, 1137, 1347, 1527, 1977, 2397, 2480
DpnII	GATC	7 cut(s)	554, 1135, 1345, 1525, 1975, 2395, 2478
DraIII	CACNNNGTG	3 cut(s)	476, 1466, 2222
EaeI	YGGCCR	1 cut(s)	1165
Eam1104I	CTCTTC	1 cut(s)	1430
EarI	CTCTTC	1 cut(s)	1430
Ecl136II	GAGCTC	3 cut(s)	124, 297, 1091
Eco105I	TACGTA	2 cut(s)	1591, 2091
Eco130I	CCWWGG	3 cut(s)	234, 1687, 1959
Eco24I	GRGCYC	4 cut(s)	126, 299, 455, 1093
Eco31I	GGTCTC	1 cut(s)	244
Eco32I	GATATC	1 cut(s)	667
Eco53kI	GAGCTC	3 cut(s)	124, 297, 1091
Eco57I	CTGAAG	2 cut(s)	804, 2367
Eco91I	GGTNACC	1 cut(s)	2126
EcoICRI	GAGCTC	3 cut(s)	124, 297, 1091
EcoO109I	RGGNCCY	3 cut(s)	451, 452, 1963
EcoO65I	GGTNACC	1 cut(s)	2126
EcoRII	CCWGG	1 cut(s)	454
EcoRV	GATATC	1 cut(s)	667
EcoT14I	CCWWGG	3 cut(s)	234, 1687, 1959
EcoT22I	ATGCAT	1 cut(s)	2067
EcoT38I	GRGCYC	4 cut(s)	126, 299, 455, 1093
ErhI	CCWWGG	3 cut(s)	234, 1687, 1959
Esp3I	CGTCTC	2 cut(s)	169, 1386
FaqI	GGGAC	2 cut(s)	515, 521
FbaI	TGATCA	3 cut(s)	1525, 2395, 2478
FblI	GTMKAC	1 cut(s)	1251
FokI	GGATG	8 cut(s)	128, 455, 806, 887, 1595, 1741, 1940, 2150
FriOI	GRGCYC	4 cut(s)	126, 299, 455, 1093
FspBI	CTAG	6 cut(s)	153, 849, 1407, 1598, 1712, 2132
GlaI	GCGC	1 cut(s)	2076
GsuI	CTGGAG	1 cut(s)	693
HaeIII	GGCC	5 cut(s)	453, 817, 1167, 1965, 2246
HapII	CCGG	5 cut(s)	180, 257, 1133, 1973, 2498
HgaI	GACGC	1 cut(s)	87
HhaI	GCGC	1 cut(s)	2077
Hin1I	GRCGYC	1 cut(s)	98
Hin6I	GCGC	1 cut(s)	2075
HinP1I	GCGC	1 cut(s)	2075
HindIII	AAGCTT	1 cut(s)	2034
HpaII	CCGG	5 cut(s)	180, 257, 1133, 1973, 2498
HphI	GGTGA	4 cut(s)	2138, 2243, 2425, 2453
Hpy166II	GTNNAC	6 cut(s)	996, 1252, 1259, 1286, 1805, 1936
Hpy8I	GTNNAC	6 cut(s)	996, 1252, 1259, 1286, 1805, 1936
Hpy99I	CGWCG	1 cut(s)	397
HpyAV	CCTTC	7 cut(s)	102, 477, 607, 793, 828, 1639, 1719
HpyCH4III	ACNGT	8 cut(s)	163, 935, 1036, 1256, 1466, 1666, 1802, 1888
HpyCH4IV	ACGT	5 cut(s)	728, 1395, 1590, 2090, 2204
HpyF10VI	GCNNNNNNNGC	9 cut(s)	104, 134, 137, 1021, 1179, 1466, 1756, 1834, 2222
HpyF3I	CTNAG	5 cut(s)	591, 1265, 1939, 1967, 2472
HpySE526I	ACGT	5 cut(s)	728, 1395, 1590, 2090, 2204
Hsp92I	GRCGYC	1 cut(s)	98
HspAI	GCGC	1 cut(s)	2075
Kpn2I	TCCGGA	2 cut(s)	1132, 2497
Ksp22I	TGATCA	3 cut(s)	1525, 2395, 2478
Kzo9I	GATC	7 cut(s)	554, 1135, 1345, 1525, 1975, 2395, 2478
LmnI	GCTCC	3 cut(s)	9, 67, 1088
Lsp1109I	GCAGC	6 cut(s)	91, 468, 1036, 1734, 2136, 2468
LweI	GCATC	6 cut(s)	562, 763, 784, 1918, 2128, 2406
MaeI	CTAG	6 cut(s)	153, 849, 1407, 1598, 1712, 2132
MaeII	ACGT	5 cut(s)	728, 1395, 1590, 2090, 2204
MaeIII	GTNAC	9 cut(s)	716, 1036, 1141, 1546, 1666, 1771, 2043, 2086, 2126
MalI	GATC	7 cut(s)	556, 1137, 1347, 1527, 1977, 2397, 2480
MboI	GATC	7 cut(s)	554, 1135, 1345, 1525, 1975, 2395, 2478
MboII	GAAGA	7 cut(s)	73, 128, 310, 992, 1120, 1307, 1447
MflI	RGATCY	2 cut(s)	554, 1345
MhlI	GDGCHC	4 cut(s)	126, 299, 455, 1093
MlsI	TGGCCA	1 cut(s)	1167
MluNI	TGGCCA	1 cut(s)	1167
MlyI	GAGTC	2 cut(s)	295, 503
MmeI	TCCRAC	2 cut(s)	1713, 2318
Mox20I	TGGCCA	1 cut(s)	1167
Mph1103I	ATGCAT	1 cut(s)	2067
MroI	TCCGGA	2 cut(s)	1132, 2497
MroXI	GAANNNNTTC	2 cut(s)	564, 1836
MscI	TGGCCA	1 cut(s)	1167
MseI	TTAA	9 cut(s)	1119, 1200, 1652, 1896, 2060, 2207, 2316, 2421, 2503
MslI	CAYNNNNRTG	1 cut(s)	1173
Msp20I	TGGCCA	1 cut(s)	1167
MspA1I	CMGCKG	2 cut(s)	104, 2459
MspCI	CTTAAG	1 cut(s)	1199
MspI	CCGG	5 cut(s)	180, 257, 1133, 1973, 2498
MspR9I	CCNGG	2 cut(s)	456, 1973
Mva1269I	GAATGC	2 cut(s)	352, 1798
MvaI	CCWGG	1 cut(s)	456
MwoI	GCNNNNNNNGC	9 cut(s)	104, 134, 137, 1021, 1179, 1466, 1756, 1834, 2222
NciI	CCSGG	1 cut(s)	1973
NcoI	CCATGG	2 cut(s)	234, 1687
NdeII	GATC	7 cut(s)	554, 1135, 1345, 1525, 1975, 2395, 2478
NlaIV	GGNNCC	2 cut(s)	453, 1341
NmuCI	GTSAC	4 cut(s)	716, 1546, 1666, 2126
NsiI	ATGCAT	1 cut(s)	2067
NspI	RCATGY	1 cut(s)	1828
PaeI	GCATGC	1 cut(s)	1828
PagI	TCATGA	1 cut(s)	568
PctI	GAATGC	2 cut(s)	352, 1798
PdmI	GAANNNNTTC	2 cut(s)	564, 1836
PleI	GAGTC	2 cut(s)	294, 503
PpsI	GAGTC	2 cut(s)	294, 503
Ppu21I	YACGTR	2 cut(s)	1591, 2091
PshBI	ATTAAT	1 cut(s)	2316
PsiI	TTATAA	3 cut(s)	684, 939, 1352
Psp124BI	GAGCTC	3 cut(s)	126, 299, 1093
Psp6I	CCWGG	1 cut(s)	454
PspEI	GGTNACC	1 cut(s)	2126
PspGI	CCWGG	1 cut(s)	454
PspN4I	GGNNCC	2 cut(s)	453, 1341
PspOMI	GGGCCC	1 cut(s)	451
PspPI	GGNCC	5 cut(s)	451, 452, 815, 1963, 2244
PstNI	CAGNNNCTG	1 cut(s)	504
PsuI	RGATCY	2 cut(s)	554, 1345
RsaI	GTAC	5 cut(s)	932, 1260, 1398, 1589, 1663
RsaNI	GTAC	5 cut(s)	931, 1259, 1397, 1588, 1662
RseI	CAYNNNNRTG	1 cut(s)	1173
SacI	GAGCTC	3 cut(s)	126, 299, 1093
SaqAI	TTAA	9 cut(s)	1119, 1200, 1652, 1896, 2060, 2207, 2316, 2421, 2503
Sau3AI	GATC	7 cut(s)	554, 1135, 1345, 1525, 1975, 2395, 2478
Sau96I	GGNCC	5 cut(s)	451, 452, 815, 1963, 2244
ScaI	AGTACT	1 cut(s)	1663
SchI	GAGTC	2 cut(s)	295, 503
ScrFI	CCNGG	2 cut(s)	456, 1973
SduI	GDGCHC	4 cut(s)	126, 299, 455, 1093
SfaNI	GCATC	6 cut(s)	562, 763, 784, 1918, 2128, 2406
SmiMI	CAYNNNNRTG	1 cut(s)	1173
SmlI	CTYRAG	4 cut(s)	290, 511, 1199, 2019
SmoI	CTYRAG	4 cut(s)	290, 511, 1199, 2019
SnaBI	TACGTA	2 cut(s)	1591, 2091
SpeI	ACTAGT	1 cut(s)	1711
SphI	GCATGC	1 cut(s)	1828
SsiI	CCGC	7 cut(s)	102, 129, 132, 135, 138, 1921, 2459
SspI	AATATT	3 cut(s)	943, 1186, 1573
SspMI	CTAG	6 cut(s)	153, 849, 1407, 1598, 1712, 2132
SstI	GAGCTC	3 cut(s)	126, 299, 1093
StyD4I	CCNGG	2 cut(s)	454, 1971
StyI	CCWWGG	3 cut(s)	234, 1687, 1959
TaaI	ACNGT	8 cut(s)	163, 935, 1036, 1256, 1466, 1666, 1802, 1888
TaiI	ACGT	5 cut(s)	731, 1398, 1593, 2093, 2207
TaqI	TCGA	9 cut(s)	114, 356, 551, 620, 1723, 1743, 1840, 2028, 2448
TatI	WGTACW	2 cut(s)	1258, 1661
TauI	GCSGC	4 cut(s)	131, 134, 137, 140
Tru1I	TTAA	9 cut(s)	1119, 1200, 1652, 1896, 2060, 2207, 2316, 2421, 2503
Tru9I	TTAA	9 cut(s)	1119, 1200, 1652, 1896, 2060, 2207, 2316, 2421, 2503
TscAI	CASTG	2 cut(s)	1175, 1261
TseFI	GTSAC	4 cut(s)	716, 1546, 1666, 2126
TseI	GCWGC	6 cut(s)	104, 481, 1024, 1747, 2149, 2456
Tsp45I	GTSAC	4 cut(s)	716, 1546, 1666, 2126
TspGWI	ACGGA	1 cut(s)	821
TspRI	CASTG	2 cut(s)	1175, 1261
Vha464I	CTTAAG	1 cut(s)	1199
VspI	ATTAAT	1 cut(s)	2316
XapI	RAATTY	5 cut(s)	47, 206, 607, 2286, 2483
XceI	RCATGY	1 cut(s)	1828
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1175
XmiI	GTMKAC	1 cut(s)	1251
XmnI	GAANNNNTTC	2 cut(s)	564, 1836
XspI	CTAG	6 cut(s)	153, 849, 1407, 1598, 1712, 2132
ZrmI	AGTACT	1 cut(s)	1663
Zsp2I	ATGCAT	1 cut(s)	2067

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.