MD16G1055100.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012004
Physical Location & Seq
Forward (+)
3887677 .. 3890917
3241 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1055100.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGAGAACCGCCGCGAAAGGCAACCAAGAGGAGGACGAGTACGACGACGAGGAGTTTGGCTCCCGGAAAGAGGGCCCTTCTTCCAATTCCAACAGCAAAGATGCCAAGAACAACGATAAGGCTAGTGCTATACGATCGAAACATTCCGTCACGGAGCAGAGAAGGAGGAGCAAGATCAATGAGAGATTCCAGATATTGAGAGATCTAATACCCCATAGTGATCAGAAAAGGGATACGGCATCATTCCTGCTAGAGGTGATCGAGTATGTCCAGTACTTACAGGAAAAAGTACACAAATATGAGGGTTCATACCAGGGTTGGAGTCCTGAGCCGACAAAATTAATGCCATGGAGAAATAGTCACTGGCGTGTTCAGAGTTTTGTTGGAAATCCTCAGGCCATAAAGAGTGACGCTGCACCAATGTTATCGTTTCCTGGAAAATTTGATGAAAGTAACATCTCTATCAATCCGAACATGCTGGCAAACAGCACACCGAATCCAGTAGAATCTGACCCTAGCAGGGATGTTGCCTCCAAAATAGTGGATCGGCAACCTGAGATAGTAAACAAGGGGATTCCGCTGCCTAGGCCTCTACCAAACATGACTCCCCCGGTCAGAAGTGATGGTGTGCTTGCCCATCCTCTCCAGGGACAAATTTCTGATGCTCAATCAACTCAGTGCCCCACCACTAGCGCTAGTGATGTACTGAGCCAACAGGAGGAGCTGACAATTGAAGGTGGAACAATAAGCATCTCAAGCGTTTACTCACAAGGGTTATTAAACTCTCTGAGTCAAGCGCTACAGAGTGCGGGTGTTGATCTGTCGCAGGCTAGTATCTCAGTGCAGATTGATCTTGGGAATCGTGCGAACCGAGGGCTGCCCTCTGTAACACCTGCCTCTAAGGATAATGATAATCCCCACTCAAGCAATCAGACGACGGCGCATTTTAGGGATGCTGGCAGCGGAGAGGACTCCGATCAAGCCCATAAGCGCCTCAAGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

335

Amino Acids

36.74

Weight (kDa)

5.8

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 45 - 94 1.3e-14 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69010
fragaria_vesca FvH4_4g29230 FvH4_4g29230
malus_domestica MD13G1056100.v1.1 MD16G1055100.v1.1
prunus_persica Prupe.1G294200_v2.0.a1 Prupe.1G294200_v2.0.a1
pyrus_communis pycom13g04970 pycom16g04800
rosa_chinensis RchiOBHm_Chr4g0437281
rosa_laevigata RLG00000006450
rosa_multiflora Rmu_sc0004339.1_g000031
rosa_roxburghii Rroxscaffold_5G00378420
rosa_rugosa Rorug04G0299200
rosa_samantha Rh4AG354000 Rh4BG363900 Rh4CG378200 Rh4DG357900
rosa_wichuraiana Rw4G031050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 901
Acc36I ACCTGC 1 cut(s) 901
AccII CGCG 1 cut(s) 14
AciI CCGC 5 cut(s) 9, 12, 578, 809, 963
AclWI GGATC 1 cut(s) 552
AcsI RAATTY 2 cut(s) 440, 654
AfaI GTAC 4 cut(s) 41, 275, 291, 705
AfeI AGCGCT 2 cut(s) 694, 798
AfiI CCNNNNNNNGG 6 cut(s) 31, 70, 253, 520, 647, 718
AgsI TTSAA 1 cut(s) 734
AjnI CCWGG 3 cut(s) 312, 433, 645
AleI CACNNNNGTG 1 cut(s) 366
AluBI AGCT 1 cut(s) 724
AluI AGCT 1 cut(s) 724
AlwI GGATC 1 cut(s) 552
Aor51HI AGCGCT 2 cut(s) 694, 798
AoxI GGCC 3 cut(s) 73, 396, 587
ApaI GGGCCC 1 cut(s) 77
ApeKI GCWGC 4 cut(s) 413, 580, 877, 960
ApoI RAATTY 2 cut(s) 440, 654
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AseI ATTAAT 1 cut(s) 341
AspA2I CCTAGG 1 cut(s) 584
AspLEI GCGC 4 cut(s) 695, 799, 943, 993
AspS9I GGNCC 2 cut(s) 73, 74
AsuC2I CCSGG 2 cut(s) 64, 611
AsuHPI GGTGA 1 cut(s) 268
AvrII CCTAGG 1 cut(s) 584
AxyI CCTNAGG 1 cut(s) 393
BaeGI GKGCMC 2 cut(s) 77, 683
BanII GRGCYC 1 cut(s) 77
BbvI GCAGC 4 cut(s) 400, 567, 864, 972
BccI CCATC 2 cut(s) 617, 645
BceAI ACGGC 2 cut(s) 252, 954
BciT130I CCWGG 3 cut(s) 314, 435, 647
BciVI GTATCC 1 cut(s) 226
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 2 cut(s) 64, 611
BfaI CTAG 7 cut(s) 123, 251, 516, 585, 690, 696, 831
BfmI CTRYAG 1 cut(s) 800
BfoI RGCGCY 3 cut(s) 696, 800, 994
BfuAI ACCTGC 1 cut(s) 901
BfuI GTATCC 1 cut(s) 226
BglII AGATCT 1 cut(s) 202
BisI GCNGC 5 cut(s) 12, 414, 581, 878, 961
BlnI CCTAGG 1 cut(s) 584
BlsI GCNGC 5 cut(s) 13, 415, 582, 879, 962
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 5 cut(s) 64, 314, 435, 611, 647
BmgT120I GGNCC 2 cut(s) 73, 74
BmiI GGNNCC 2 cut(s) 61, 75
BmrFI CCNGG 5 cut(s) 64, 314, 435, 611, 647
BmsI GCATC 5 cut(s) 91, 248, 652, 759, 943
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 3 cut(s) 739, 907, 980
BpuMI CCSGG 2 cut(s) 64, 611
BsaJI CCNNGG 6 cut(s) 313, 347, 584, 609, 646, 871
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 343, 373
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 70, 253, 520, 647, 718
Bse1I ACTGG 3 cut(s) 271, 368, 500
Bse21I CCTNAGG 1 cut(s) 393
BseBI CCWGG 3 cut(s) 314, 435, 647
BseDI CCNNGG 6 cut(s) 313, 347, 584, 609, 646, 871
BseGI GGATG 3 cut(s) 529, 637, 958
BseLI CCNNNNNNNGG 6 cut(s) 31, 70, 253, 520, 647, 718
BseMII CTCAG 7 cut(s) 318, 407, 546, 689, 698, 779, 852
BseNI ACTGG 3 cut(s) 271, 368, 500
BseRI GAGGAG 4 cut(s) 44, 65, 181, 734
BseSI GKGCMC 2 cut(s) 77, 683
BseXI GCAGC 4 cut(s) 400, 567, 864, 972
BsgI GTGCAG 2 cut(s) 399, 863
Bsh1236I CGCG 1 cut(s) 14
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 3 cut(s) 75, 398, 589
BsiEI CGRYCG 1 cut(s) 137
BsiSI CCGG 2 cut(s) 64, 611
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 6 cut(s) 31, 70, 253, 520, 647, 718
BsmFI GGGAC 1 cut(s) 663
BsnI GGCC 3 cut(s) 75, 398, 589
Bsp120I GGGCCC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 77, 683
Bsp143I GATC 9 cut(s) 134, 174, 202, 220, 258, 544, 817, 850, 976
Bsp19I CCATGG 1 cut(s) 347
BspACI CCGC 5 cut(s) 9, 12, 578, 809, 963
BspANI GGCC 3 cut(s) 75, 398, 589
BspCNI CTCAG 7 cut(s) 319, 406, 547, 688, 699, 780, 851
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 2 cut(s) 61, 75
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 1 cut(s) 552
BsrI ACTGG 3 cut(s) 271, 368, 500
BssECI CCNNGG 6 cut(s) 313, 347, 584, 609, 646, 871
BssMI GATC 9 cut(s) 134, 174, 202, 220, 258, 544, 817, 850, 976
BssT1I CCWWGG 2 cut(s) 347, 584
Bst2UI CCWGG 3 cut(s) 314, 435, 647
BstC8I GCNNGC 4 cut(s) 480, 633, 828, 958
BstDEI CTNAG 8 cut(s) 327, 393, 555, 675, 707, 788, 838, 900
BstDSI CCRYGG 1 cut(s) 347
BstENI CCTNNNNNAGG 1 cut(s) 251
BstF5I GGATG 3 cut(s) 529, 637, 958
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 3 cut(s) 696, 800, 994
BstHHI GCGC 4 cut(s) 695, 799, 943, 993
BstKTI GATC 9 cut(s) 137, 177, 205, 223, 261, 547, 820, 853, 979
BstMBI GATC 9 cut(s) 134, 174, 202, 220, 258, 544, 817, 850, 976
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 2 cut(s) 586, 756
BstNI CCWGG 3 cut(s) 314, 435, 647
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 5 cut(s) 62, 312, 433, 609, 645
BstSFI CTRYAG 1 cut(s) 800
BstSLI GKGCMC 2 cut(s) 77, 683
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 4 cut(s) 400, 567, 864, 972
BstX2I RGATCY 1 cut(s) 202
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 1 cut(s) 202
Bsu36I CCTNAGG 1 cut(s) 393
BsuI GTATCC 1 cut(s) 226
BsuRI GGCC 3 cut(s) 75, 398, 589
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 3 cut(s) 529, 637, 958
BtsIMutI CAGTG 3 cut(s) 361, 683, 846
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 4 cut(s) 480, 633, 828, 958
CfoI GCGC 4 cut(s) 695, 799, 943, 993
Cfr13I GGNCC 2 cut(s) 73, 74
CseI GACGC 1 cut(s) 419
Csp6I GTAC 4 cut(s) 40, 274, 290, 704
CviAII CATG 3 cut(s) 348, 475, 601
CviQI GTAC 4 cut(s) 40, 274, 290, 704
DdeI CTNAG 8 cut(s) 327, 393, 555, 675, 707, 788, 838, 900
DpnI GATC 9 cut(s) 136, 176, 204, 222, 260, 546, 819, 852, 978
DpnII GATC 9 cut(s) 134, 174, 202, 220, 258, 544, 817, 850, 976
Eco130I CCWWGG 2 cut(s) 347, 584
Eco147I AGGCCT 1 cut(s) 589
Eco24I GRGCYC 1 cut(s) 77
Eco47III AGCGCT 2 cut(s) 694, 798
Eco81I CCTNAGG 1 cut(s) 393
EcoNI CCTNNNNNAGG 1 cut(s) 251
EcoO109I RGGNCCY 2 cut(s) 73, 74
EcoRII CCWGG 3 cut(s) 312, 433, 645
EcoT14I CCWWGG 2 cut(s) 347, 584
EcoT38I GRGCYC 1 cut(s) 77
ErhI CCWWGG 2 cut(s) 347, 584
FaeI CATG 3 cut(s) 351, 478, 604
FaqI GGGAC 1 cut(s) 663
FatI CATG 3 cut(s) 347, 474, 600
FauI CCCGC 1 cut(s) 802
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 5 cut(s) 12, 414, 581, 878, 961
FokI GGATG 3 cut(s) 536, 624, 965
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 5 cut(s) 12, 414, 581, 878, 961
FspBI CTAG 7 cut(s) 123, 251, 516, 585, 690, 696, 831
GlaI GCGC 4 cut(s) 694, 798, 942, 992
GluI GCNGC 5 cut(s) 12, 414, 581, 878, 961
GsuI CTGGAG 1 cut(s) 629
HaeII RGCGCY 3 cut(s) 696, 800, 994
HaeIII GGCC 3 cut(s) 75, 398, 589
HapII CCGG 2 cut(s) 64, 611
HgaI GACGC 1 cut(s) 419
HhaI GCGC 4 cut(s) 695, 799, 943, 993
Hin1II CATG 3 cut(s) 351, 478, 604
Hin6I GCGC 4 cut(s) 693, 797, 941, 991
HinP1I GCGC 4 cut(s) 693, 797, 941, 991
HinfI GANTC 9 cut(s) 186, 322, 496, 506, 574, 604, 790, 859, 971
HpaII CCGG 2 cut(s) 64, 611
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 3 cut(s) 292, 565, 763
Hpy188I TCNGA 9 cut(s) 225, 375, 471, 511, 617, 661, 789, 933, 976
Hpy188III TCNNGA 3 cut(s) 190, 326, 997
Hpy8I GTNNAC 3 cut(s) 292, 565, 763
Hpy99I CGWCG 3 cut(s) 47, 50, 940
HpyAV CCTTC 3 cut(s) 87, 156, 728
HpyCH4IV ACGT 1 cut(s) 1001
HpyCH4V TGCA 2 cut(s) 416, 844
HpyF10VI GCNNNNNNNGC 2 cut(s) 586, 756
HpyF3I CTNAG 8 cut(s) 327, 393, 555, 675, 707, 788, 838, 900
HpySE526I ACGT 1 cut(s) 1001
Hsp92II CATG 3 cut(s) 351, 478, 604
HspAI GCGC 4 cut(s) 693, 797, 941, 991
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 9 cut(s) 134, 174, 202, 220, 258, 544, 817, 850, 976
LmnI GCTCC 4 cut(s) 65, 154, 168, 721
Lsp1109I GCAGC 4 cut(s) 400, 567, 864, 972
LweI GCATC 5 cut(s) 91, 248, 652, 759, 943
MaeI CTAG 7 cut(s) 123, 251, 516, 585, 690, 696, 831
MaeII ACGT 1 cut(s) 1001
MaeIII GTNAC 5 cut(s) 148, 359, 407, 452, 886
MalI GATC 9 cut(s) 136, 176, 204, 222, 260, 546, 819, 852, 978
MboI GATC 9 cut(s) 134, 174, 202, 220, 258, 544, 817, 850, 976
MboII GAAGA 1 cut(s) 72
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 2 cut(s) 77, 683
MluCI AATT 5 cut(s) 85, 338, 440, 654, 729
MlyI GAGTC 4 cut(s) 331, 598, 799, 965
MmeI TCCRAC 3 cut(s) 114, 299, 364
MseI TTAA 2 cut(s) 341, 779
MslI CAYNNNNRTG 2 cut(s) 297, 366
MspA1I CMGCKG 2 cut(s) 580, 963
MspI CCGG 2 cut(s) 64, 611
MspR9I CCNGG 5 cut(s) 64, 314, 435, 611, 647
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 3 cut(s) 314, 435, 647
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 2 cut(s) 586, 756
NciI CCSGG 2 cut(s) 64, 611
NcoI CCATGG 1 cut(s) 347
NdeII GATC 9 cut(s) 134, 174, 202, 220, 258, 544, 817, 850, 976
NlaIII CATG 3 cut(s) 351, 478, 604
NlaIV GGNNCC 2 cut(s) 61, 75
NmuCI GTSAC 3 cut(s) 148, 359, 407
NspI RCATGY 1 cut(s) 478
OliI CACNNNNGTG 1 cut(s) 366
PaqCI CACCTGC 1 cut(s) 901
PceI AGGCCT 1 cut(s) 589
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 868
PfeI GAWTC 5 cut(s) 186, 496, 506, 574, 859
PfoI TCCNGGA 2 cut(s) 62, 433
PkrI GCNGC 5 cut(s) 13, 415, 582, 879, 962
Ple19I CGATCG 1 cut(s) 137
PleI GAGTC 4 cut(s) 330, 598, 798, 965
PpsI GAGTC 4 cut(s) 330, 598, 798, 965
PshBI ATTAAT 1 cut(s) 341
Psp6I CCWGG 3 cut(s) 312, 433, 645
PspGI CCWGG 3 cut(s) 312, 433, 645
PspN4I GGNNCC 2 cut(s) 61, 75
PspOMI GGGCCC 1 cut(s) 73
PspPI GGNCC 2 cut(s) 73, 74
PsuI RGATCY 1 cut(s) 202
PvuI CGATCG 1 cut(s) 137
RsaI GTAC 4 cut(s) 41, 275, 291, 705
RsaNI GTAC 4 cut(s) 40, 274, 290, 704
RseI CAYNNNNRTG 2 cut(s) 297, 366
SaqAI TTAA 2 cut(s) 341, 779
SatI GCNGC 5 cut(s) 12, 414, 581, 878, 961
Sau3AI GATC 9 cut(s) 134, 174, 202, 220, 258, 544, 817, 850, 976
Sau96I GGNCC 2 cut(s) 73, 74
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 4 cut(s) 331, 598, 799, 965
ScrFI CCNGG 5 cut(s) 64, 314, 435, 611, 647
SduI GDGCHC 2 cut(s) 77, 683
SetI ASST 6 cut(s) 258, 556, 726, 739, 895, 1004
SfaNI GCATC 5 cut(s) 91, 248, 652, 759, 943
SfcI CTRYAG 1 cut(s) 800
SmiMI CAYNNNNRTG 2 cut(s) 297, 366
SmlI CTYRAG 3 cut(s) 754, 922, 995
SmoI CTYRAG 3 cut(s) 754, 922, 995
Sse9I AATT 5 cut(s) 85, 338, 440, 654, 729
SseBI AGGCCT 1 cut(s) 589
SsiI CCGC 5 cut(s) 9, 12, 578, 809, 963
SspMI CTAG 7 cut(s) 123, 251, 516, 585, 690, 696, 831
StuI AGGCCT 1 cut(s) 589
StyD4I CCNGG 5 cut(s) 62, 312, 433, 609, 645
StyI CCWWGG 2 cut(s) 347, 584
TaiI ACGT 1 cut(s) 1004
TaqI TCGA 2 cut(s) 137, 261
TasI AATT 5 cut(s) 85, 338, 440, 654, 729
TatI WGTACW 3 cut(s) 273, 289, 703
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 5 cut(s) 186, 496, 506, 574, 859
Tru1I TTAA 2 cut(s) 341, 779
Tru9I TTAA 2 cut(s) 341, 779
TscAI CASTG 3 cut(s) 368, 683, 846
TseFI GTSAC 3 cut(s) 148, 359, 407
TseI GCWGC 4 cut(s) 413, 580, 877, 960
Tsp45I GTSAC 3 cut(s) 148, 359, 407
TspDTI ATGAA 2 cut(s) 297, 462
TspGWI ACGGA 2 cut(s) 136, 167
TspRI CASTG 3 cut(s) 368, 683, 846
VspI ATTAAT 1 cut(s) 341
XagI CCTNNNNNAGG 1 cut(s) 251
XapI RAATTY 2 cut(s) 440, 654
XceI RCATGY 1 cut(s) 478
XmaJI CCTAGG 1 cut(s) 584
XspI CTAG 7 cut(s) 123, 251, 516, 585, 690, 696, 831
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.