MD16G1061000.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002060
Physical Location & Seq
Reverse (-)
4359259 .. 4363428
4170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1061000.v1.1.491

Sequence Viewer

Length: 2010 bp
ATGGCAACAAAAAAATTAAAAGTCAAAAACCCGGCAAAAAAACGACAACTTCAAAATGCACAGCAGCTCTTTACTGAAACGCCCCTTGAAAAATGGGAGCAAAAGTCGAATCTTCCTTTTAAGATTCCCAAAGTTGTTAACGATTTGCAATCATTGAAAACTCAGAAACCCAAAAAAATCTTGTACTTCAGTTCGACTGCATCACTCAAAGTTTGCAGCTTTCTTCCTTTGTTCTCACAAAGTTTCTTCATTTTTCATGCTGCGTGGGAAATTGGGATGAAAGAAATGAAACAAGTGAGGCTGTTGGCAATTCTGTTAGTTGTTGTTGCAACCCTTCAAGTTGAGAAGGCAAGCTCGCATGGAGATCACCCTCTTTCCAGAATTGCTATTCATAGAGCAACCTTTGCACTTGATGAGCCTGCTTACATTCAAGCCTCTCCTTCCGTTCTTGGATTAAGGGGGCAGAACTCAGAGTGGGTGAAGTTGGAATTTGGTACCGAAAACCCGAAAATCGATGATTGGATTGGAGTGTTTTCTCCTGCTAATTTCAGTGCTTCCACCTGCCCTGAGGAGAATCCGAGAGTTTTTCCTCCATTTTTGTGTTCTGCGCCGATTAAGTTTCAGTATGCGAATTACTCCACTCCCGACTACAAAGACACCGGAAAGGGGTTCTTGAAGCTTCAGCTCATCAACCAGAGATCAGAGTTCTCTTTCGCACTATTTTCTGGCGGTTTATTGAATCCAAGGGTGGTGGCGGTGTCAAATCATATAGCTTTCGCCAATCCAGATGCACCAGTTTACCCGCGCATAGCACAAGGAAAAGAATGGAATGAAATGACCGTAACATGGACAAGTGGATATGGGATCAATGACGCAGAACCTTTTGTTGAATGGGGTCCGAGAGGAGAAACTACGCGTTCCCCTGCTGTGACGCTAACTTTTGATCGTCGTAGCTTGTGTGCACCAGCAAGAACAGTGGGATGGCGTGACCCTGGATTCATACATACTAGTTTTCTGAAGGAGTTGTGGCCAAGCAGACAGTACACGTACAAGCTGGGGCATAGATTGTTTAATGGTACATACATTTGGAGTCAAACGTATCAGTTTAGAGCATCCCCTTATCCTGGTCAAAATTCTTTACAGCGTGTAGTCATTTTCGGTGACATGGGAAAGGATGAAGCTGATGGTTCCAATGAATACAACAACTTCCAGCCAGGTTCGCTTAACACTACTAAGCAACTTATTCGTGACTTGAAAAACATCGATATAGTTGAACCAATAGCATCAACTGTTCCTTACATGATTGCAAGTGGAAACCATGAACGAGACTGGCCAGGAACAGGGTTCTTTCTATCGAGAACATGGATTCAGGAGGTGAATGCGGTGTGTTGGCTGAAAATATGTTCTATGTTCCCACAGAGAACAGAGCTAAGTTTTGGTACAAGACTGGACTACGGCATGTTTCGCTTCTGCATAGCAGACACGGAACATGACTGGAGAGAGGGCACGGAGCAGTACAAGTTCATCGAGCACTGCTTAGCATCATTCTGGGATACTCTTCAGGATTTCTATTACGCGAAAGAAGGATCATTTGCAGAACCAATGGGGAGGGAGAGCCTCCAAAAACTCTGGCAAAAGTATAAGGTCGACATTGGCATCTATGGCCATGTCCATAACTACGAAAGAACCTGTCCCATATACCAGAATATTTGCACCGATGAAGAGAAACGCCATTACAAGGGCAGCTTGAATGGAACAATACATGTGGTTGCTGGTGGTGCAGGAGCAAGGCTGTCAACGTTTACCGCCATCCAAACGAAATGGAGTGTTTTCAAAGACTACGACCATGGATTCGTGAAGCTCACAGCATTCGATCACTCGAACCTTTTGTTCGAGTACAAGAAGAGCAGGGACGGGCAGGTTTACGACTCGTTCAGAATATCCAGAGATTACAGGGACATCTTGGCCTGCACTGCTGATAGCTGCCCTAGCACCACACTTGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

670

Amino Acids

76.37

Weight (kDa)

8.75

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 143 - 261 5.7e-41 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 269 - 370 7.5e-11 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 403 - 560 6.3e-06 Calcineurin-like phosphoesterase
Metallophos_C PF14008 585 - 644 1.7e-15 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 569
Acc36I ACCTGC 2 cut(s) 569, 1909
Acc65I GGTACC 1 cut(s) 494
AccB1I GGYRCC 1 cut(s) 494
AccI GTMKAC 1 cut(s) 1647
AccII CGCG 3 cut(s) 805, 916, 1577
AciI CCGC 5 cut(s) 729, 755, 803, 1382, 1806
AclI AACGTT 1 cut(s) 1799
AclWI GGATC 2 cut(s) 872, 1594
AcoI YGGCCR 3 cut(s) 1028, 1331, 1663
AcsI RAATTY 2 cut(s) 488, 1132
AcuI CTGAAG 4 cut(s) 172, 665, 1037, 1544
AfaI GTAC 8 cut(s) 185, 496, 1043, 1049, 1078, 1441, 1517, 1898
AfiI CCNNNNNNNGG 5 cut(s) 666, 846, 1124, 1340, 1738
AflIII ACRYGT 3 cut(s) 914, 1044, 1762
AhlI ACTAGT 1 cut(s) 1007
AjnI CCWGG 4 cut(s) 991, 1123, 1213, 1333
Alw21I GWGCWC 2 cut(s) 964, 1533
Alw26I GTCTC 1 cut(s) 1320
Alw44I GTGCAC 1 cut(s) 960
AlwI GGATC 2 cut(s) 872, 1594
AoxI GGCC 4 cut(s) 1028, 1331, 1663, 1965
ApaLI GTGCAC 1 cut(s) 960
ApeKI GCWGC 5 cut(s) 64, 216, 260, 1743, 1983
ApoI RAATTY 2 cut(s) 488, 1132
ArsI GACNNNNNNTTYG 2 cut(s) 1835, 1867
Asp718I GGTACC 1 cut(s) 494
AspLEI GCGC 2 cut(s) 610, 807
AspS9I GGNCC 1 cut(s) 896
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 4 cut(s) 359, 490, 1172, 1387
AvaII GGWCC 1 cut(s) 896
AxyI CCTNAGG 1 cut(s) 567
BaeGI GKGCMC 2 cut(s) 964, 1508
BalI TGGCCA 3 cut(s) 1030, 1333, 1665
BanI GGYRCC 1 cut(s) 494
Bbv12I GWGCWC 2 cut(s) 964, 1533
BbvI GCAGC 5 cut(s) 76, 228, 247, 1755, 1970
BccI CCATC 3 cut(s) 975, 1178, 1817
BceAI ACGGC 1 cut(s) 1471
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BciT130I CCWGG 4 cut(s) 993, 1125, 1215, 1335
BciVI GTATCC 1 cut(s) 1546
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 1320
BcuI ACTAGT 1 cut(s) 1007
BfaI CTAG 2 cut(s) 1008, 1989
BfuAI ACCTGC 2 cut(s) 569, 1909
BfuI GTATCC 1 cut(s) 1546
BisI GCNGC 5 cut(s) 65, 217, 261, 1744, 1984
BlpI GCTNAGC 1 cut(s) 1537
BlsI GCNGC 5 cut(s) 66, 218, 262, 1745, 1985
Bme1390I CCNGG 5 cut(s) 32, 993, 1125, 1215, 1335
Bme18I GGWCC 1 cut(s) 896
BmgT120I GGNCC 1 cut(s) 896
BmiI GGNNCC 3 cut(s) 496, 897, 1189
BmrFI CCNGG 5 cut(s) 32, 993, 1125, 1215, 1335
BmsI GCATC 6 cut(s) 209, 778, 1121, 1292, 1550, 1665
BpmI CTGGAG 1 cut(s) 1516
Bpu1102I GCTNAGC 1 cut(s) 1537
BpuMI CCSGG 1 cut(s) 32
Bsa29I ATCGAT 2 cut(s) 513, 1263
BsaAI YACGTR 1 cut(s) 1047
BsaJI CCNNGG 3 cut(s) 743, 991, 1846
BsaWI WCCGGW 1 cut(s) 659
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 5 cut(s) 666, 846, 1124, 1340, 1738
Bse1I ACTGG 4 cut(s) 794, 1334, 1452, 1499
Bse21I CCTNAGG 1 cut(s) 567
BseBI CCWGG 4 cut(s) 993, 1125, 1215, 1335
BseCI ATCGAT 2 cut(s) 513, 1263
BseDI CCNNGG 3 cut(s) 743, 991, 1846
BseGI GGATG 5 cut(s) 282, 986, 1112, 1180, 1809
BseLI CCNNNNNNNGG 5 cut(s) 666, 846, 1124, 1340, 1738
BseMII CTCAG 3 cut(s) 176, 483, 558
BseNI ACTGG 4 cut(s) 794, 1334, 1452, 1499
BseRI GAGGAG 2 cut(s) 584, 918
BseSI GKGCMC 2 cut(s) 964, 1508
BseXI GCAGC 5 cut(s) 76, 228, 247, 1755, 1970
BseYI CCCAGC 1 cut(s) 1054
BsgI GTGCAG 2 cut(s) 1800, 1954
Bsh1236I CGCG 3 cut(s) 805, 916, 1577
BshFI GGCC 4 cut(s) 1030, 1333, 1665, 1967
BshNI GGYRCC 1 cut(s) 494
BshVI ATCGAT 2 cut(s) 513, 1263
BsiHKAI GWGCWC 2 cut(s) 964, 1533
BsiSI CCGG 2 cut(s) 32, 660
BslFI GGGAC 3 cut(s) 1677, 1925, 1970
BslI CCNNNNNNNGG 5 cut(s) 666, 846, 1124, 1340, 1738
BsmAI GTCTC 1 cut(s) 1320
BsmFI GGGAC 3 cut(s) 1677, 1925, 1970
BsmI GAATGC 2 cut(s) 1384, 1868
BsnI GGCC 4 cut(s) 1030, 1333, 1665, 1967
Bsp1286I GDGCHC 3 cut(s) 964, 1508, 1533
Bsp143I GATC 6 cut(s) 364, 698, 864, 943, 1586, 1873
Bsp1720I GCTNAGC 1 cut(s) 1537
Bsp19I CCATGG 1 cut(s) 1846
BspACI CCGC 5 cut(s) 729, 755, 803, 1382, 1806
BspANI GGCC 4 cut(s) 1030, 1333, 1665, 1967
BspCNI CTCAG 3 cut(s) 175, 482, 559
BspDI ATCGAT 2 cut(s) 513, 1263
BspFNI CGCG 3 cut(s) 805, 916, 1577
BspHI TCATGA 1 cut(s) 2006
BspLI GGNNCC 3 cut(s) 496, 897, 1189
BspMI ACCTGC 2 cut(s) 569, 1909
BspPI GGATC 2 cut(s) 872, 1594
BspQI GCTCTTC 1 cut(s) 1898
BspT107I GGYRCC 1 cut(s) 494
BsrI ACTGG 4 cut(s) 794, 1334, 1452, 1499
BssECI CCNNGG 3 cut(s) 743, 991, 1846
BssMI GATC 6 cut(s) 364, 698, 864, 943, 1586, 1873
BssT1I CCWWGG 2 cut(s) 743, 1846
Bst2UI CCWGG 4 cut(s) 993, 1125, 1215, 1335
Bst4CI ACNGT 4 cut(s) 841, 976, 1041, 1291
Bst6I CTCTTC 3 cut(s) 1563, 1716, 1898
BstAPI GCANNNNNTGC 1 cut(s) 404
BstBAI YACGTR 1 cut(s) 1047
BstC8I GCNNGC 4 cut(s) 352, 356, 420, 1969
BstDEI CTNAG 6 cut(s) 162, 469, 567, 1233, 1430, 1537
BstDSI CCRYGG 1 cut(s) 1846
BstF5I GGATG 5 cut(s) 282, 986, 1112, 1180, 1809
BstFNI CGCG 3 cut(s) 805, 916, 1577
BstHHI GCGC 2 cut(s) 610, 807
BstKTI GATC 6 cut(s) 367, 701, 867, 946, 1589, 1876
BstMAI GTCTC 1 cut(s) 1320
BstMBI GATC 6 cut(s) 364, 698, 864, 943, 1586, 1873
BstMWI GCNNNNNNNGC 7 cut(s) 404, 1219, 1464, 1662, 1778, 1973, 1989
BstNI CCWGG 4 cut(s) 993, 1125, 1215, 1335
BstNSI RCATGY 2 cut(s) 1462, 1766
BstSCI CCNGG 5 cut(s) 30, 991, 1123, 1213, 1333
BstSLI GKGCMC 2 cut(s) 964, 1508
BstUI CGCG 3 cut(s) 805, 916, 1577
BstV1I GCAGC 5 cut(s) 76, 228, 247, 1755, 1970
Bsu15I ATCGAT 2 cut(s) 513, 1263
Bsu36I CCTNAGG 1 cut(s) 567
BsuI GTATCC 1 cut(s) 1546
BsuRI GGCC 4 cut(s) 1030, 1333, 1665, 1967
BsuTUI ATCGAT 2 cut(s) 513, 1263
BtgI CCRYGG 1 cut(s) 1846
BtsCI GGATG 5 cut(s) 282, 986, 1112, 1180, 1809
BtsI GCAGTG 2 cut(s) 1531, 1971
BtsIMutI CAGTG 4 cut(s) 556, 981, 1531, 1971
BveI ACCTGC 2 cut(s) 569, 1909
Cac8I GCNNGC 4 cut(s) 352, 356, 420, 1969
CciI TCATGA 1 cut(s) 2006
CfoI GCGC 2 cut(s) 610, 807
Cfr13I GGNCC 1 cut(s) 896
ClaI ATCGAT 2 cut(s) 513, 1263
CseI GACGC 2 cut(s) 881, 940
Csp6I GTAC 8 cut(s) 184, 495, 1042, 1048, 1077, 1440, 1516, 1897
CspCI CAANNNNNGTGG 6 cut(s) 732, 767, 957, 992, 1746, 1781
CviQI GTAC 8 cut(s) 184, 495, 1042, 1048, 1077, 1440, 1516, 1897
DdeI CTNAG 6 cut(s) 162, 469, 567, 1233, 1430, 1537
DpnI GATC 6 cut(s) 366, 700, 866, 945, 1588, 1875
DpnII GATC 6 cut(s) 364, 698, 864, 943, 1586, 1873
EaeI YGGCCR 3 cut(s) 1028, 1331, 1663
Eam1104I CTCTTC 3 cut(s) 1563, 1716, 1898
EarI CTCTTC 3 cut(s) 1563, 1716, 1898
Eco130I CCWWGG 2 cut(s) 743, 1846
Eco47I GGWCC 1 cut(s) 896
Eco57I CTGAAG 4 cut(s) 172, 665, 1037, 1544
Eco81I CCTNAGG 1 cut(s) 567
EcoRII CCWGG 4 cut(s) 991, 1123, 1213, 1333
EcoT14I CCWWGG 2 cut(s) 743, 1846
ErhI CCWWGG 2 cut(s) 743, 1846
FalI AAGNNNNNCTT 4 cut(s) 656, 688, 1730, 1762
FaqI GGGAC 3 cut(s) 1677, 1925, 1970
FauI CCCGC 1 cut(s) 810
FblI GTMKAC 1 cut(s) 1647
Fnu4HI GCNGC 5 cut(s) 65, 217, 261, 1744, 1984
FokI GGATG 5 cut(s) 289, 993, 1099, 1187, 1796
Fsp4HI GCNGC 5 cut(s) 65, 217, 261, 1744, 1984
FspBI CTAG 2 cut(s) 1008, 1989
GlaI GCGC 2 cut(s) 609, 806
GluI GCNGC 5 cut(s) 65, 217, 261, 1744, 1984
GsaI CCCAGC 1 cut(s) 1058
GsuI CTGGAG 1 cut(s) 1516
HaeIII GGCC 4 cut(s) 1030, 1333, 1665, 1967
HapII CCGG 2 cut(s) 32, 660
HgaI GACGC 2 cut(s) 881, 940
HhaI GCGC 2 cut(s) 610, 807
Hin6I GCGC 2 cut(s) 608, 805
HinP1I GCGC 2 cut(s) 608, 805
HincII GTYRAC 3 cut(s) 139, 1648, 1797
HindII GTYRAC 3 cut(s) 139, 1648, 1797
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 9 cut(s) 109, 124, 574, 739, 996, 1090, 1366, 1851, 1928
HpaI GTTAAC 1 cut(s) 139
HpaII CCGG 2 cut(s) 32, 660
HphI GGTGA 4 cut(s) 359, 490, 1172, 1387
Hpy166II GTNNAC 8 cut(s) 139, 799, 962, 1044, 1648, 1797, 1803, 1924
Hpy188I TCNGA 7 cut(s) 165, 472, 579, 703, 900, 1017, 1937
Hpy8I GTNNAC 8 cut(s) 139, 799, 962, 1044, 1648, 1797, 1803, 1924
Hpy99I CGWCG 1 cut(s) 951
HpyAV CCTTC 5 cut(s) 340, 344, 450, 1012, 1577
HpyCH4III ACNGT 4 cut(s) 841, 976, 1041, 1291
HpyCH4IV ACGT 3 cut(s) 1046, 1097, 1799
HpyF10VI GCNNNNNNNGC 7 cut(s) 404, 1219, 1464, 1662, 1778, 1973, 1989
HpyF3I CTNAG 6 cut(s) 162, 469, 567, 1233, 1430, 1537
HpySE526I ACGT 3 cut(s) 1046, 1097, 1799
HspAI GCGC 2 cut(s) 608, 805
KpnI GGTACC 1 cut(s) 498
KspAI GTTAAC 1 cut(s) 139
Kzo9I GATC 6 cut(s) 364, 698, 864, 943, 1586, 1873
LguI GCTCTTC 1 cut(s) 1898
LmnI GCTCC 3 cut(s) 97, 1510, 1784
Lsp1109I GCAGC 5 cut(s) 76, 228, 247, 1755, 1970
LweI GCATC 6 cut(s) 209, 778, 1121, 1292, 1550, 1665
MaeI CTAG 2 cut(s) 1008, 1989
MaeII ACGT 3 cut(s) 1046, 1097, 1799
MaeIII GTNAC 5 cut(s) 841, 928, 986, 1160, 1247
MalI GATC 6 cut(s) 366, 700, 866, 945, 1588, 1875
MboI GATC 6 cut(s) 364, 698, 864, 943, 1586, 1873
MboII GAAGA 6 cut(s) 104, 215, 238, 1550, 1733, 1915
MhlI GDGCHC 3 cut(s) 964, 1508, 1533
MlsI TGGCCA 3 cut(s) 1030, 1333, 1665
MluCI AATT 8 cut(s) 14, 270, 309, 381, 488, 544, 631, 1132
MluI ACGCGT 1 cut(s) 914
MluNI TGGCCA 3 cut(s) 1030, 1333, 1665
MlyI GAGTC 2 cut(s) 1099, 1922
MmeI TCCRAC 1 cut(s) 465
Mox20I TGGCCA 3 cut(s) 1030, 1333, 1665
MscI TGGCCA 3 cut(s) 1030, 1333, 1665
MseI TTAA 7 cut(s) 17, 120, 138, 455, 615, 1071, 1224
MslI CAYNNNNRTG 1 cut(s) 598
Msp20I TGGCCA 3 cut(s) 1030, 1333, 1665
MspI CCGG 2 cut(s) 32, 660
MspR9I CCNGG 5 cut(s) 32, 993, 1125, 1215, 1335
Mva1269I GAATGC 2 cut(s) 1384, 1868
MvaI CCWGG 4 cut(s) 993, 1125, 1215, 1335
MvnI CGCG 3 cut(s) 805, 916, 1577
MwoI GCNNNNNNNGC 7 cut(s) 404, 1219, 1464, 1662, 1778, 1973, 1989
NciI CCSGG 1 cut(s) 32
NcoI CCATGG 1 cut(s) 1846
NdeII GATC 6 cut(s) 364, 698, 864, 943, 1586, 1873
NlaIV GGNNCC 3 cut(s) 496, 897, 1189
NmuCI GTSAC 4 cut(s) 928, 986, 1160, 1247
NspI RCATGY 2 cut(s) 1462, 1766
PagI TCATGA 1 cut(s) 2006
PaqCI CACCTGC 1 cut(s) 569
PciI ACATGT 1 cut(s) 1762
PciSI GCTCTTC 1 cut(s) 1898
PctI GAATGC 2 cut(s) 1384, 1868
PfeI GAWTC 7 cut(s) 109, 124, 574, 739, 996, 1366, 1851
PkrI GCNGC 5 cut(s) 66, 218, 262, 1745, 1985
PleI GAGTC 2 cut(s) 1098, 1922
PpsI GAGTC 2 cut(s) 1098, 1922
Ppu21I YACGTR 1 cut(s) 1047
PscI ACATGT 1 cut(s) 1762
Psp1406I AACGTT 1 cut(s) 1799
Psp6I CCWGG 4 cut(s) 991, 1123, 1213, 1333
PspFI CCCAGC 1 cut(s) 1054
PspGI CCWGG 4 cut(s) 991, 1123, 1213, 1333
PspN4I GGNNCC 3 cut(s) 496, 897, 1189
PspPI GGNCC 1 cut(s) 896
RsaI GTAC 8 cut(s) 185, 496, 1043, 1049, 1078, 1441, 1517, 1898
RsaNI GTAC 8 cut(s) 184, 495, 1042, 1048, 1077, 1440, 1516, 1897
RseI CAYNNNNRTG 1 cut(s) 598
SalI GTCGAC 1 cut(s) 1646
SapI GCTCTTC 1 cut(s) 1898
SaqAI TTAA 7 cut(s) 17, 120, 138, 455, 615, 1071, 1224
SatI GCNGC 5 cut(s) 65, 217, 261, 1744, 1984
Sau3AI GATC 6 cut(s) 364, 698, 864, 943, 1586, 1873
Sau96I GGNCC 1 cut(s) 896
SchI GAGTC 2 cut(s) 1099, 1922
ScrFI CCNGG 5 cut(s) 32, 993, 1125, 1215, 1335
SduI GDGCHC 3 cut(s) 964, 1508, 1533
SfaNI GCATC 6 cut(s) 209, 778, 1121, 1292, 1550, 1665
SinI GGWCC 1 cut(s) 896
SmiMI CAYNNNNRTG 1 cut(s) 598
SpeI ACTAGT 1 cut(s) 1007
Sse9I AATT 8 cut(s) 14, 270, 309, 381, 488, 544, 631, 1132
SsiI CCGC 5 cut(s) 729, 755, 803, 1382, 1806
SspI AATATT 1 cut(s) 1708
SspMI CTAG 2 cut(s) 1008, 1989
StyD4I CCNGG 5 cut(s) 30, 991, 1123, 1213, 1333
StyI CCWWGG 2 cut(s) 743, 1846
TaaI ACNGT 4 cut(s) 841, 976, 1041, 1291
TaiI ACGT 3 cut(s) 1049, 1100, 1802
TasI AATT 8 cut(s) 14, 270, 309, 381, 488, 544, 631, 1132
TatI WGTACW 4 cut(s) 183, 1041, 1515, 1896
TfiI GAWTC 7 cut(s) 109, 124, 574, 739, 996, 1366, 1851
Tru1I TTAA 7 cut(s) 17, 120, 138, 455, 615, 1071, 1224
Tru9I TTAA 7 cut(s) 17, 120, 138, 455, 615, 1071, 1224
TscAI CASTG 4 cut(s) 556, 981, 1538, 1978
TseFI GTSAC 4 cut(s) 928, 986, 1160, 1247
TseI GCWGC 5 cut(s) 64, 216, 260, 1743, 1983
Tsp45I GTSAC 4 cut(s) 928, 986, 1160, 1247
TspGWI ACGGA 3 cut(s) 433, 1499, 1523
TspRI CASTG 4 cut(s) 556, 981, 1538, 1978
VneI GTGCAC 1 cut(s) 960
VpaK11BI GGWCC 1 cut(s) 896
XapI RAATTY 2 cut(s) 488, 1132
XceI RCATGY 2 cut(s) 1462, 1766
XmiI GTMKAC 1 cut(s) 1647
XspI CTAG 2 cut(s) 1008, 1989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.